CN111558305A - Adjustable nano molecular diameter selective filter and screening process - Google Patents
Adjustable nano molecular diameter selective filter and screening process Download PDFInfo
- Publication number
- CN111558305A CN111558305A CN201910387925.9A CN201910387925A CN111558305A CN 111558305 A CN111558305 A CN 111558305A CN 201910387925 A CN201910387925 A CN 201910387925A CN 111558305 A CN111558305 A CN 111558305A
- Authority
- CN
- China
- Prior art keywords
- pool
- filter
- screening
- connecting port
- tank
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000012216 screening Methods 0.000 title claims abstract description 86
- 238000000034 method Methods 0.000 title claims abstract description 17
- 238000002156 mixing Methods 0.000 claims abstract description 66
- 230000001954 sterilising effect Effects 0.000 claims abstract description 7
- 238000004140 cleaning Methods 0.000 claims abstract description 5
- 239000011259 mixed solution Substances 0.000 claims abstract description 4
- 238000003825 pressing Methods 0.000 claims abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 22
- 239000000463 material Substances 0.000 claims description 19
- 239000000243 solution Substances 0.000 claims description 16
- 239000003960 organic solvent Substances 0.000 claims description 14
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 13
- 239000003085 diluting agent Substances 0.000 claims description 10
- 239000007788 liquid Substances 0.000 claims description 10
- 239000000126 substance Substances 0.000 claims description 9
- 238000003780 insertion Methods 0.000 claims description 7
- 230000037431 insertion Effects 0.000 claims description 7
- 239000003480 eluent Substances 0.000 claims description 6
- 238000004659 sterilization and disinfection Methods 0.000 claims description 6
- 239000002504 physiological saline solution Substances 0.000 claims description 4
- 238000007789 sealing Methods 0.000 claims description 4
- 239000002775 capsule Substances 0.000 claims description 3
- 239000000203 mixture Substances 0.000 abstract description 3
- 230000000149 penetrating effect Effects 0.000 abstract 1
- 150000003384 small molecules Chemical class 0.000 description 15
- 238000002474 experimental method Methods 0.000 description 13
- 230000000694 effects Effects 0.000 description 7
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 5
- 239000003814 drug Substances 0.000 description 5
- 229910021389 graphene Inorganic materials 0.000 description 5
- 238000013461 design Methods 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 238000011068 loading method Methods 0.000 description 3
- 230000000259 anti-tumor effect Effects 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 239000011557 critical solution Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 238000011089 mechanical engineering Methods 0.000 description 1
- 239000002086 nanomaterial Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 239000002861 polymer material Substances 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 238000002626 targeted therapy Methods 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
- 229910021642 ultra pure water Inorganic materials 0.000 description 1
- 239000012498 ultrapure water Substances 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 238000003466 welding Methods 0.000 description 1
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F23/00—Mixing according to the phases to be mixed, e.g. dispersing or emulsifying
- B01F23/80—After-treatment of the mixture
- B01F23/808—Filtering the mixture
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D29/00—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor
- B01D29/01—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor with flat filtering elements
- B01D29/03—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor with flat filtering elements self-supporting
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D29/00—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor
- B01D29/50—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor with multiple filtering elements, characterised by their mutual disposition
- B01D29/56—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor with multiple filtering elements, characterised by their mutual disposition in series connection
- B01D29/58—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor with multiple filtering elements, characterised by their mutual disposition in series connection arranged concentrically or coaxially
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D29/00—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor
- B01D29/96—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor in which the filtering elements are moved between filtering operations; Particular measures for removing or replacing the filtering elements; Transport systems for filters
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82B—NANOSTRUCTURES FORMED BY MANIPULATION OF INDIVIDUAL ATOMS, MOLECULES, OR LIMITED COLLECTIONS OF ATOMS OR MOLECULES AS DISCRETE UNITS; MANUFACTURE OR TREATMENT THEREOF
- B82B3/00—Manufacture or treatment of nanostructures by manipulation of individual atoms or molecules, or limited collections of atoms or molecules as discrete units
- B82B3/0004—Apparatus specially adapted for the manufacture or treatment of nanostructural devices or systems or methods for manufacturing the same
Landscapes
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Engineering & Computer Science (AREA)
- Crystallography & Structural Chemistry (AREA)
- Nanotechnology (AREA)
- Manufacturing & Machinery (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The utility model provides a nanometer molecular diameter selectivity filter and screening process with adjustable, the filter includes the container, the container is including mixing the pond, screening pond and little molecular pool triplex, mix pond and screening pond, set up the filter intercommunication respectively between screening pond and the little molecular pool, the filter lies in the middle part on length direction and sets up the penetrating cylindrical connecting hole in two, the outer wall in mixing pond and screening pond is cup jointed respectively at the inherent two of connecting hole, or cup joint the outer wall in screening pond and little molecular pool, be on a parallel with the two on the filter and set up the jack in the middle part, the filter plate is embedded in the jack. The screening process comprises the following steps: 1) premixing; 2) mixing uniformly; 3) installing a filter plate; 4) pushing and pressing the mixed solution; 5) collecting a sample; 6) and (5) cleaning and sterilizing the container. The invention can solve the technical problems of inaccurate nanometer molecular diameter judgment result of an electron microscope, lack of electron microscope instruments and various using steps.
Description
Technical Field
The invention relates to the field of nano-molecular diameter selection, in particular to an adjustable nano-molecular diameter selective filter and a screening process.
Background
Nano-molecules have a wide range of applications in many areas, such as chemical industry, materials science, and medicine. Due to the special physical and chemical properties of the nano molecules, the functions which can not be completed by a plurality of non-nano molecules can be realized, such as high-efficiency catalytic capability, good photothermal effect, good photodynamic effect, good drug loading capability and the like. The special functions of some of the nano-molecules, such as photothermal effect, photodynamic effect and drug loading capacity, are currently applied to medicine, and the most common application aspect is the targeted therapy of tumors. However, one of the more critical solutions to achieve these biomedical functions is to determine their physicochemical properties, such as size, thickness, surface potential, surface modifying molecules, etc. One of the important physical properties is the size of the nano-molecule, i.e., the diameter of the nano-molecule.
Taking graphene oxide nano-molecules as an example, the diameter of the graphene oxide nano-molecules is directly related to the role thereof in targeting anti-tumor. The bigger the diameter of the nano molecule is, the more difficult it is to enter tumor cells, and the photothermal effect is worse and worse, however, if the diameter of the nano molecule is too small, the drug loading capacity of the nano molecule is affected to a certain extent, the detection is also more difficult, and the uncertainty factor of the experiment is increased. Therefore, the diameter of the ideal graphene oxide nano molecule should be controlled within a certain value range, and in many current antitumor experiments using graphene oxide nano molecules, the diameter of the adopted nano graphene oxide is mostly between 50nm and 100 nm. In most of the experiments at present, the diameter of the molecule can only be used as a measurement result, and the most common measurement method is scanning electron microscope, and the diameter of the manufactured molecule is determined by taking a picture through the electron microscope. The working principle of the scanning electron microscope is disclosed in the related data and patents, and the detailed description is omitted in the present invention.
In the existing experimental process, after the preparation of the nano-molecules is completed, the nano-molecules are often directly placed under an electron microscope to determine the size, which may be completely undesirable (such as molecules with a larger proportion of excessively large or excessively small diameter), and more cases are non-uniform molecular diameters, wherein a certain proportion of the molecular diameters are desirable and undesirable, and the cases are mainly due to the fact that the subjective factors are large because the molecular diameters are not screened in the experimental process. This will certainly have a great influence on the experimental results, and since the nano-molecules with undesirable molecular diameters cannot achieve the expected experimental functions, the experiment may generate false positive or false negative results, which may affect the determination of the experimental results. In addition, in practical experiments, an electron microscope belongs to a relatively scarce resource, is very complex to use, and needs to go through complicated material taking and sheet making links. The invention can be used for screening the diameter of the molecule, the probability that the screened molecule meets the requirement is higher, the use frequency of an electron microscope can be reduced, and the experimental process is simpler and more economical.
Disclosure of Invention
In order to overcome the defects and shortcomings of the technology, the invention provides an adjustable nanometer molecular diameter selective filter and a screening process, and aims to solve the technical problems that an electron microscope is inaccurate in nanometer molecular diameter judgment result, an electron microscope instrument is deficient, and the use steps are multiple.
The invention adopts the following technical scheme:
an adjustable nanometer molecular diameter selective filter comprises a container, wherein the container comprises a mixing pool, a screening pool and a small molecular pool, the mixing pool and the screening pool, the screening pool and the small molecular pool are respectively communicated with each other through filters,
the container is in a capsule shape, the screening tank is a cylindrical shell with two through ends, the mixing tank and the small molecule tank are both shells with one through end and one spherical surface closed end, the filter is a cuboid,
the filter is characterized in that a cylindrical connecting hole with two through ends is arranged in the middle of the filter in the length direction, the two ends of the connecting hole are respectively sleeved with the outer walls of the mixing tank and the screening tank or sleeved with the outer walls of the screening tank and the small molecule tank, jacks are arranged in the middle of the filter in parallel to the two ends of the filter, one side of each jack is opened, the other side of each jack is closed, a filter plate is embedded into each jack, the filter plate comprises an edge part and a central piece fixed in the middle of the edge part, the central piece is overlapped with the outline of the hole wall of the connecting hole, and the edge part and the hole wall of each jack of the filter are;
the wall of the mixing pool is provided with an air vent and a connector, the wall of the screening pool is provided with a connector, the wall of the micromolecule pool is provided with an air vent and a connector,
each connecting port and each air vent are provided with a valve;
the central piece is a perforated filter screen or a non-perforated plate, and when the central piece is the perforated filter screen, the aperture of the filter screen between the mixing tank and the screening tank is larger than the aperture between the screening tank and the small molecule tank.
And sealing gaskets are fixed on two sides of the edge part.
Mix pond front end and be the pool wall middle part and the edge of sphere and set up connector one and connector two, set up blow vent one on the lateral wall respectively, the screening pond lateral wall sets up connector three, the pool wall middle part that the micromolecule pond end is the sphere sets up connector four, sets up blow vent two on the lateral wall.
The filter is characterized in that concave parts are arranged on two sides of an opening of the jack along the insertion direction of the filter plate, and the tail part of the filter plate is located in the concave parts.
A screening process of an adjustable nanometer molecular diameter selective filter comprises the following steps:
1) premixing: valves of a first connecting port, a second connecting port and a first air vent of the mixing tank are opened, other valves of the container are closed, and a filter plate with a non-porous plate as a central plate is inserted into a jack of the filter; injecting a to-be-processed nano molecular solution into the mixing pool from the first connecting port by using an injector, injecting a diluent into the mixing pool from the second connecting port by using another injector, and closing valves of the first connecting port and the second connecting port;
2) uniformly mixing: placing the container into an ultrasonic pool or a vibration pool for vibration and uniform mixing, and closing a valve of a vent when the vent is not deflated after the nano molecular solution and the diluent in the mixing pool are fully and uniformly mixed;
3) the installation center piece is the filter plate of foraminiferous filter screen: vertically erecting the container to enable the solution in the mixing tank to be concentrated at the front end, taking out the two filter plates with the central sheets being non-porous plates in the step 1), and respectively replacing the filter plates with the central sheets being filter screens with holes, wherein the aperture of the filter screen between the mixing tank and the screening tank is larger than that between the screening tank and the small molecule tank;
4) pushing and pressing the mixed solution: pushing air into the mixing pool from a second connecting port of the mixing pool by using an injector, and repeating the steps for a plurality of times until all the liquid in the mixing pool and the liquid in the screening pool in the step 2) are extruded into the small molecule pool;
5) collecting a sample: a certain amount of pure water, normal saline or organic solvent is sucked by an injector and injected from a third connecting port, and after the substances in the screening pool are dissolved in the pure water, the normal saline or the organic solvent, a new injector is taken to suck the solution in the screening pool out of the third connecting port; the liquid in the small molecule pool is discarded;
or: taking a syringe to absorb a certain amount of pure water or normal saline or organic solvent, injecting the pure water or normal saline or organic solvent from the third connecting port and the fourth connecting port in parts, and after substances in the screening pool and the small molecular pool are dissolved in the pure water or normal saline or organic solvent, taking a new syringe to suck out the solution in the screening pool and the small molecular pool from the third connecting port and the fourth connecting port respectively;
6) cleaning and sterilizing the container: and valves of the first connecting port and the fourth connecting port of the container are opened, other valves are closed, the material eluent and the pure water are injected from the fourth connecting port by the injector in sequence, the material eluent or the pure water flows out from the first connecting port after sequentially passing through the small molecular pool, the screening pool and the mixing pool, and the disinfection and the sterilization are carried out after repeating for a plurality of times.
The diluent in the step 1) is pure water or normal saline.
The invention has the following positive and beneficial effects:
1) molecules that can obtain the planned diameter;
2) the ability to screen molecules in a gradient;
3) the probability that the molecules after the molecular diameter is screened by using the invention meet the requirements is higher, the use frequency of an electron microscope can be reduced, the experiment is more economic and rapid, the reliability of the experiment can be enhanced, and the control group of the nano molecules with different diameters can be set according to the experiment requirements, so that the experiment process is simpler and more economical.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic view of the filter of the present invention;
FIG. 3 is a schematic structural view of a filter plate according to the present invention;
FIG. 4 is a cross-sectional view taken along line A-A of FIG. 3;
FIG. 5 is a schematic view of the filter plate of the present invention inserted into the receptacle;
FIG. 6 is a schematic view of the connection structure of two filters according to the present invention;
FIG. 7 is a schematic representation of a mixing operation using the filter of the present invention.
The reference numbers: 1-mixing pool, 11-connecting port one, 12-connecting port two, 13-ventilating port one, 2-screening pool, 21-connecting port three, 3-small molecule pool, 31-connecting port four, 32-ventilating port two, 4-filter, 41-connecting hole, 42-jack, 43-concave part, 5-filter plate, 51-edge part, 52-central piece, 53-sealing pad and 6-injector.
Detailed Description
The technical solution of the present invention will be systematically described in conjunction with the drawings in the text, and the described embodiments are some, but not all embodiments of the present invention, and can be flexibly applied in practical research works as needed. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations and positional relationships based on the orientations and positional relationships shown in the drawings, and are only described in terms of simple orientations according to the drawings, and are not mandatory in terms of mechanical engineering, and should not be limited in actual construction, operation, and application, and therefore the description of the orientations herein should not be construed as limiting the application of the present invention. Furthermore, the terms "first," "second," and "third" are used herein for descriptive purposes only and not for purposes of limitation.
In the description of the present invention, it should be further noted that, since some structures of the present invention are completely new in design, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" should be interpreted broadly, as well as the same mechanical structures in the drawings. For example, the connection may be a fixed connection, a detachable connection, or an integral connection; the connection can be mechanical connection or welding; the connection can be direct through various means, indirect through screws, or through various engineering tools. The screw as drawn in the drawing can be any other connecting tool such as a rivet and the like which can realize the practical clinical application of the invention in the real manufacturing. Therefore, specific meanings of the above terms in the present invention can be understood in specific cases by those of ordinary skill in the art, and should not be limited by the drawings herein as actually made.
The invention aims to design a molecular filter for controlling the diameter of a nanometer molecule, after the design shown by the invention is finished, the nanometer molecule meeting the diameter requirement is remained in a screening pool, and the situation that the nanometer molecule in the screening pool is mixed and polluted again by the nanometer molecule not meeting the diameter requirement in the correct operation process can not occur, so that the experimental efficiency is effectively improved, and the experimental reliability is increased.
Referring to fig. 1-6, an adjustable selective filter with nanometer molecular diameter comprises a container, wherein the container comprises a mixing pool 1, a screening pool 2 and a small molecular pool 3, the mixing pool 1 and the screening pool 2, and the screening pool 2 and the small molecular pool 3 are respectively communicated with each other through a filter 4,
referring to fig. 1 and 2, the container is in the shape of a capsule, the screening tank 2 is a cylindrical shell with two through ends, the mixing tank 1 and the small molecule tank 3 are both shells with one through end and one closed spherical end, the filter 4 is a cuboid,
referring to fig. 2, 3, 4 and 5, a cylindrical connecting hole 41 with two through ends is arranged in the middle of the filter 4 in the length direction, referring to fig. 6, two ends of the connecting hole 41 are respectively sleeved on the outer walls of the mixing tank 1 and the screening tank 2, or sleeved on the outer walls of the screening tank 2 and the small molecule tank 3, jacks 42 are arranged in the middle of the filter 4 parallel to the two ends, the jacks 42 are opened on one side of the filter 4, the other sides of the filter 4 are closed, a filter plate 5 is embedded in the jacks 42, the filter plate 5 comprises an edge part 51 and a central piece 52 fixed in the middle of the edge part 51, the hole wall 52 is overlapped with the hole wall profile of the connecting hole 41, and the edge part 51 is arranged in a sealing manner with the jacks 42 of the filter 4;
referring to fig. 1, the wall of the mixing pool 1 is provided with an air vent and a connector, the wall of the screening pool 2 is provided with a connector, the wall of the micromolecule pool 3 is provided with an air vent and a connector,
each connecting port and each air vent are provided with a valve;
the central sheet 52 is a perforated filter screen or a non-perforated plate, and when the central sheet 52 is a perforated filter screen, the aperture of the filter screen between the mixing tank 1 and the screening tank 2 is larger than the aperture between the screening tank 2 and the small molecule tank 3.
The edge portion 51 is fixed with gaskets 53 on both sides.
The filter 4 is provided with concave parts 43 at two sides of the opening of the jack 42 along the inserting direction of the filter plate 5, and the tail part of the filter plate 5 is positioned in the concave parts 43.
As shown in fig. 1, which is an overall schematic diagram of the molecular filter for controlling the diameter of the nano-molecules, the filter container 1 is mainly composed of three parts, namely a mixing pool 1, a screening pool 2 and a small-diameter molecular pool 3.
It is emphasized that the present invention can be used for screening molecules of a certain molecular diameter, and can also be used for gradient screening of molecular diameters, and the present invention is also applicable if the molecules with diameters in a plurality of intervals need to be separated conveniently and rapidly.
The following description will be given by taking the screening of molecules with a certain molecular diameter as an example, and the diameter of the nano-molecules screened in this example is 50-100nm, but it should be emphasized that this is only an example, and the screening in other intervals can be accomplished by applying the present invention.
Taking the diameter of the screened nano molecule as 50-100nm as an example, in the using process, the prepared pre-screened material generally needs to be diluted and uniformly mixed firstly, and needs to be uniformly mixed by ultrasound or vibration when necessary, so as to increase the filtering efficiency, extract the material molecule meeting the diameter requirement to the maximum extent and save the material. The molecules of the pre-screening material are generally placed in an injector, because the storage concentration is generally larger after the material is prepared, the injector is connected with a first connecting port 11, and ultrapure water or normal saline (or other organic solvents and the like according to the experimental requirements) prepared for dilution is placed in another injector.
This paragraph describes the specific configuration of the mixing tank. The syringe and corresponding material were connected as described above. The mixing pool mainly comprises a first connecting port 11, a second connecting port 12, a first air vent 13 and valves on all the ports, wherein the first connecting port 11 and the second connecting port 12 are standard needle tube interfaces of medical injectors and can be connected with the medical injectors which are commonly used in the market at present. The valve is used for controlling the opening/closing of the first connecting port 11 and the second connecting port 12, and has the same structure and principle as the existing medical valve. The inner wall surface of the mixing tank 1 should be a smooth plane to avoid unnecessary waste caused by excessive residual nano-material molecules. The first vent 13 of the mixing tank 11 is used for adjusting the air pressure in the mixing tank 1. An air vent net is arranged at the outlet of the first air vent 13, the air vent net is made of a commercially available waterproof air-permeable film (breathing paper) and is a novel high polymer material, and the net structure only allows gas to pass through but does not allow liquid, namely material molecules to pass through.
The screening tank 2 comprises a tank body, filters 4 at two ends, a connecting port III 21 and a valve. The filter 4 includes a connection hole 41 and an insertion hole 42. The wall end of the mixing tank 1 is nested in the connecting hole 41 (correspondingly, the wall end of the small molecule tank 7 is also nested in the connecting hole 41). Both ends of the wall of the screening pool 2 are nested in the connecting holes 41 at both ends. Connection port three 21 is also a medical syringe universal connection port for use in final collection of material. The valve in this section is used for controlling the opening/closing of the connecting port three 21, and has the same structure and principle as the existing medical valve.
It is emphasized that the present invention utilizes alternative filter plates 5 to achieve the screening of molecules of a certain interval of diameter. The present invention prepares a set of filter screens with different pore sizes as the central sheet 52, such as 10nm, 20nm, 50nm, 100nm, 150nm, 200nm, etc.
Use embodiment 1
In this example, molecules with a diameter in the interval of 50-100nm were selected. For the purposes of the present description, one filter plate 5 with a 50nm filter and one filter plate 5 with a 100nm filter are used, while two filter plates 5 with non-perforated plates are required. The number in front of the filter screen represents the maximum molecular diameter that the filter screen can pass through, for example, a filter screen with 50nm pore size, and the rest is analogized in the same way. The thickness of the screen should be designed to be less than or equal to the thickness of the receptacle 42, and the edges of the receptacle 42 should be chamfered to facilitate removal of the filter plate 5. The filter plates 5 with different aperture sizes of filter screens are inserted into the insertion holes 42 according to different purposes. Specific implementations will be described in the following paragraphs.
The small molecule pool 3 comprises a pool body, a second air vent 32, a valve, a fourth connecting port 31 and a valve 7.5.
An adjustable nanomolecular diameter selective filter of the present invention can be used for:
1. molecules of the planned diameter are obtained. As described in the first working example, the diameter of the nanomolecule entering the screening pool 2 is no more than 100nm, the material molecules with the molecular diameter less than 50nm enter the small-diameter molecular pool 3, and finally the molecular diameter in the screening pool 2 is 50-100nm, i.e. the molecules with the required diameter are left in the screening pool 2;
2. the molecules were screened in a gradient. As described in the first embodiment, the diameters of the nano molecules in the mixing tank 1, the screening tank 2 and the small molecule tank 3 are distributed in a gradient manner, the gradient setting is the diameter of the filter screen, and the extraction processes of the nano molecules in different gradient levels are all performed by injecting and dissolving a certain amount of proper solvent and then sucking out the solvent;
3. it is easier to adjust the size for different control experiments. And part of experiments need to compare the effects of different diameters of nano molecules applied respectively, so that the experiments can be simplified by applying the equipment, and the reliability is improved.
The use method of the adjustable nanometer molecular diameter selective filter comprises the following steps:
taking the screening of particles with 50-100nm diameter as an example: the function of the invention is mainly divided into three stages of premixing, sample collection and cleaning. In the premixing stage, as shown in fig. 7, the material to be screened is in the injector 6 of the first connection port 11 and the diluent is in the injector 6 of the second connection port 12.
A screening process of an adjustable nanometer molecular diameter selective filter comprises the following steps:
1) premixing: the valves of the first connecting port 11, the second connecting port 12 and the first air vent 12 of the mixing tank 1 are opened, the other valves of the container are closed, and a filter plate 5 with a central sheet 52 of a non-porous plate material is inserted into the insertion hole 42 of the filter 4; injecting a to-be-treated nano molecular solution into the mixing pool 1 from the first connecting port 11 by using an injector 6, injecting a diluent into the mixing pool 1 from the second connecting port 12 by using another injector 6, and closing valves of the first connecting port 11 and the second connecting port 12;
2) uniformly mixing: placing the container into an ultrasonic pool or a vibration pool for vibration and uniform mixing, and closing a valve of a first air vent 13 when the first air vent 13 does not deflate after the nano molecular solution and the diluent in the mixing pool 1 are fully and uniformly mixed;
3) the mounting center piece 52 is a filter plate 5 with a filter screen with holes: vertically erecting the container to enable the solution in the mixing tank 1 to be concentrated at the front end, taking out the filter plates 5 of which the two central sheets 52 are non-porous plates in the step 1), and respectively replacing the filter plates 5 of which the central sheets 52 are porous filter screens, wherein the aperture of the filter screen between the mixing tank 1 and the screening tank 2 is larger than that between the screening tank 2 and the small molecule tank 3;
4) pushing and pressing the mixed solution: pushing air from a second connecting port 12 of the mixing pool 1 by using an injector 6, and repeating for many times until all the liquid in the mixing pool 1 and the liquid in the screening pool 2 in the step 2) are extruded into the small molecule pool 3;
5) collecting a sample: a certain amount of pure water or normal saline or organic solvent is sucked by the syringe 6 and injected from the third connecting port 21, and after the substances in the screening pool 2 are dissolved in the pure water or normal saline or organic solvent, a new syringe 6 is taken to suck the solution in the screening pool 2 out from the third connecting port 21; the liquid in the small molecule pool 3 is discarded;
or: a certain amount of pure water or physiological saline or organic solvent is sucked by the syringe 6 and injected from a third connecting port 21 and a fourth connecting port 31 in a subsection mode, and after the substances in the screening pool 2 and the small molecular pool 3 are dissolved in the pure water or the physiological saline or the organic solvent, a new syringe 6 is taken to suck out the solutions in the screening pool 2 and the small molecular pool 3 from the third connecting port 21 and the fourth connecting port 31 respectively;
6) cleaning and sterilizing the container: and valves of the first connecting port 11 and the fourth connecting port 31 of the container are opened, other valves are closed, the material eluent and the pure water are injected from the fourth connecting port 31 by the injector 6 in sequence, the material eluent or the pure water flows out from the first connecting port 11 after sequentially passing through the small molecular pool 3, the screening pool 2 and the mixing pool 1, and the disinfection and the sterilization are carried out after repeated for multiple times.
The diluent in the step 1) is pure water or normal saline.
The present invention uses a plurality of syringes 6, which are not mixed unless the syringes have no effect on the experimental results in two operations. Based on the strict principle of experiments, the plurality of syringes 6 of the present invention are all disposable syringes containing only one substance.
Claims (6)
1. An adjustable nanometer molecular diameter selective filter is characterized by comprising a container, wherein the container comprises a mixing pool (1), a screening pool (2) and a small molecular pool (3), the mixing pool (1), the screening pool (2) and the small molecular pool (3) are respectively communicated with one another through filters (4),
the container is in a capsule shape, the screening tank (2) is a cylindrical shell with two through ends, the mixing tank (1) and the small molecular tank (3) are both shells with one through end and one closed spherical end, the filter (4) is a cuboid,
the filter (4) is positioned in the middle in the length direction and is provided with two through cylindrical connecting holes (41), two ends of the connecting holes (41) are respectively sleeved with the outer walls of the mixing tank (1) and the screening tank (2) or the outer walls of the screening tank (2) and the small molecular tank (3), the filter (4) is provided with inserting holes (42) in the middle in parallel with the two ends, the inserting holes (42) are opened at one side and closed at the other side of the filter (4), a filter plate (5) is embedded into the inserting holes (42), the filter plate (5) comprises an edge part (51) and a central piece (52) fixed in the middle of the edge part (51), the central piece (52) is overlapped with the profile of the connecting holes (41), and the edge part (51) and the hole wall of the inserting holes (42) of the filter (4) are hermetically arranged;
the wall of the mixing tank (1) is provided with an air vent and a connector, the wall of the screening tank (2) is provided with a connector, the wall of the small molecular tank (3) is provided with an air vent and a connector,
each connecting port and each air vent are provided with a valve;
the central piece (52) is a perforated filter screen or a non-perforated plate, and when the central piece (52) is the perforated filter screen, the aperture of the filter screen between the mixing tank (1) and the screening tank (2) is larger than the aperture between the screening tank (2) and the small molecular tank (3).
2. The tunable nanomolecular diameter selective filter according to claim 1, wherein the edge portion (51) is fixed with a sealing gasket (53) at both sides.
3. The adjustable selective filter with nanometer molecular diameter according to claim 1, wherein the mixing tank (1) is provided with a first connecting port (11) and a second connecting port (12) at the center and the edge of the spherical tank wall at the front end, respectively, and a first vent port (13) on the side wall, the screening tank (2) is provided with a third connecting port (21) on the side wall, the small molecular tank (3) is provided with a fourth connecting port (31) at the center and a second vent port (32) on the side wall, respectively, of the spherical tank wall at the tail end.
4. The adjustable nanometer molecular diameter selective filter according to claim 1, wherein the filter (4) is provided with an inner concave portion (43) along the insertion direction of the filter plate (5) at both sides of the opening of the insertion hole (42), and the tail portion of the filter plate (5) is positioned in the inner concave portion (43).
5. A screening process of an adjustable nanometer molecular diameter selective filter is characterized by comprising the following steps:
1) premixing: valves of a first connecting port (11), a second connecting port (12) and a first air vent (12) of the mixing tank (1) are opened, other valves of the container are closed, and a filter plate (5) with a central sheet (52) being a non-porous plate is inserted into an insertion hole (42) of the filter (4); injecting a to-be-treated nano molecular solution into the mixing pool (1) from the first connecting port (11) by using an injector (6), injecting a diluent into the mixing pool (1) from the second connecting port (12) by using another injector (6), and closing valves of the first connecting port (11) and the second connecting port (12);
2) uniformly mixing: placing the container into an ultrasonic pool or a vibration pool for vibration and uniform mixing, and closing a valve of a first air vent (13) when the first air vent (13) does not deflate after the nano molecular solution and the diluent in the mixing pool (1) are fully and uniformly mixed;
3) the mounting center piece (52) is a filter plate (5) with a hole filter screen: vertically erecting the container to enable the solution in the mixing tank (1) to be concentrated at the front end, taking out the filter plates (5) of which the two central pieces (52) are non-porous plates in the step 1), respectively replacing the filter plates (5) of which the central pieces (52) are porous filter screens, wherein the aperture of the filter screen between the mixing tank (1) and the screening tank (2) is larger than that between the screening tank (2) and the small molecular tank (3);
4) pushing and pressing the mixed solution: pushing air from a second connecting port (12) of the mixing pool (1) by using an injector (6), and repeating for many times until all the liquid in the mixing pool (1) and the liquid in the screening pool (2) in the step 2) are extruded into the small molecular pool (3);
5) collecting a sample: a certain amount of pure water or normal saline or organic solvent is sucked by the injector (6) and injected from a third connecting port (21), and after the substances in the screening pool (2) are dissolved in the pure water or normal saline or organic solvent, a new injector (6) is taken to suck the solution in the screening pool (2) from the third connecting port (21); the liquid in the small molecular pool (3) is discarded;
or: a certain amount of pure water or physiological saline or organic solvent is sucked by an injector (6) and injected from a third connecting port (21) and a fourth connecting port (31) in a dividing mode, and after substances in the screening pool (2) and the small molecular pool (3) are dissolved in the pure water or the physiological saline or the organic solvent, a new injector (6) is taken to suck out the solution in the screening pool (2) and the small molecular pool (3) from the third connecting port (21) and the fourth connecting port (31) respectively;
6) cleaning and sterilizing the container: valves of a first connecting port (11) and a fourth connecting port (31) of the container are opened, other valves are closed, a material eluent and pure water are injected from the fourth connecting port (31) in sequence by using an injector (6), the material eluent or the pure water flows out from the first connecting port (11) after passing through a small molecular pool (3), a screening pool (2) and a mixing pool (1) in sequence, and the disinfection and the sterilization are carried out after the steps are repeated for a plurality of times.
6. The screening process of an adjustable nanometer molecular diameter selective filter according to claim 5, wherein the diluent in the step 1) is pure water or normal saline.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910387925.9A CN111558305A (en) | 2019-05-10 | 2019-05-10 | Adjustable nano molecular diameter selective filter and screening process |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910387925.9A CN111558305A (en) | 2019-05-10 | 2019-05-10 | Adjustable nano molecular diameter selective filter and screening process |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111558305A true CN111558305A (en) | 2020-08-21 |
Family
ID=72069847
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910387925.9A Pending CN111558305A (en) | 2019-05-10 | 2019-05-10 | Adjustable nano molecular diameter selective filter and screening process |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111558305A (en) |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040069714A1 (en) * | 2002-10-11 | 2004-04-15 | Ferguson Gary William | Filter apparatus and methods to capture a desired amount of material from a sample suspension for monolayer deposition, analysis or other uses |
| DE102009001756A1 (en) * | 2009-03-23 | 2009-07-30 | Agilent Technologies Inc., Santa Clara | Filter screen e.g. dirt filter, for sample separation device e.g. high-performance liquid chromatography system, has filter arranged at inlet and/or outlet of separating column and impermeable for particles with size above threshold value |
| US20100108580A1 (en) * | 2008-11-04 | 2010-05-06 | Lukasik Stephen J | Molecular Separators, Concentrators, and Detectors Preparatory to Sensor Operation, and Methods of Minimizing False Positives in Sensor Operations |
| CN203155104U (en) * | 2013-04-16 | 2013-08-28 | 青岛中人智业生物科技有限公司 | Molecular film filtering tank capable of favorably improving natural pigment quality |
| CN103706556A (en) * | 2013-12-02 | 2014-04-09 | 无锡合众信息科技有限公司 | Material filter |
| CN203790621U (en) * | 2014-03-21 | 2014-08-27 | 四川什邡市三高生化实业有限公司 | Active carbon de-coloring device |
| CN106334364A (en) * | 2016-08-23 | 2017-01-18 | 中国医学科学院整形外科医院 | Multi-aperture fat filtrator |
| WO2018066979A1 (en) * | 2016-10-07 | 2018-04-12 | 울산과학기술원 | Apparatus for nanoparticle detection and method for nanoparticle detection using same |
| CN210544451U (en) * | 2019-05-10 | 2020-05-19 | 华中科技大学同济医学院附属协和医院 | Adjustable nano molecular diameter selective filter |
-
2019
- 2019-05-10 CN CN201910387925.9A patent/CN111558305A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040069714A1 (en) * | 2002-10-11 | 2004-04-15 | Ferguson Gary William | Filter apparatus and methods to capture a desired amount of material from a sample suspension for monolayer deposition, analysis or other uses |
| US20100108580A1 (en) * | 2008-11-04 | 2010-05-06 | Lukasik Stephen J | Molecular Separators, Concentrators, and Detectors Preparatory to Sensor Operation, and Methods of Minimizing False Positives in Sensor Operations |
| DE102009001756A1 (en) * | 2009-03-23 | 2009-07-30 | Agilent Technologies Inc., Santa Clara | Filter screen e.g. dirt filter, for sample separation device e.g. high-performance liquid chromatography system, has filter arranged at inlet and/or outlet of separating column and impermeable for particles with size above threshold value |
| CN203155104U (en) * | 2013-04-16 | 2013-08-28 | 青岛中人智业生物科技有限公司 | Molecular film filtering tank capable of favorably improving natural pigment quality |
| CN103706556A (en) * | 2013-12-02 | 2014-04-09 | 无锡合众信息科技有限公司 | Material filter |
| CN203790621U (en) * | 2014-03-21 | 2014-08-27 | 四川什邡市三高生化实业有限公司 | Active carbon de-coloring device |
| CN106334364A (en) * | 2016-08-23 | 2017-01-18 | 中国医学科学院整形外科医院 | Multi-aperture fat filtrator |
| WO2018066979A1 (en) * | 2016-10-07 | 2018-04-12 | 울산과학기술원 | Apparatus for nanoparticle detection and method for nanoparticle detection using same |
| CN210544451U (en) * | 2019-05-10 | 2020-05-19 | 华中科技大学同济医学院附属协和医院 | Adjustable nano molecular diameter selective filter |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DE60022025T2 (en) | APPENDIX FOR BREAKING CELLS | |
| EP1480749B1 (en) | Microfluid system | |
| EP3294244B1 (en) | System for treatment of wounds using serum | |
| DE2101423A1 (en) | ||
| CN210544451U (en) | Adjustable nano molecular diameter selective filter | |
| EP3090768A1 (en) | Device with inlet section for treatment of a biological liquid | |
| US5861094A (en) | Filter for biological particle separation | |
| DE3818776A1 (en) | Process for the cultivation of cells in a fermenter, and fermenter intended for carrying out the process | |
| DE202020105018U1 (en) | Integrated intracavity gas circulation processing device for laparoscopic surgery | |
| CN111558305A (en) | Adjustable nano molecular diameter selective filter and screening process | |
| EP2823879B1 (en) | Emulsion preparation device and emulsion preparation method | |
| DE102018103634B3 (en) | Media filtration device and method for media filtration | |
| CN207306686U (en) | A kind of tumor sample suction tube | |
| CN210170091U (en) | Liposuction needle | |
| CN210442125U (en) | Collection member and collection device | |
| JP2007191512A (en) | Manufacturing process of porous bead | |
| WO2004027015A1 (en) | Device and method for processing biological material | |
| CN201108672Y (en) | Suction filtering equipment | |
| DE102016123658B4 (en) | Filtration device and method for the enrichment of targets and the subsequent release of biogenic agents | |
| CN207478131U (en) | A kind of stoste filters core pipe | |
| CN210170524U (en) | Detachable liposuction bottle for negative pressure liposuction | |
| CN109797093A (en) | A kind of consumptive material, device and method for extracting nucleic acid for nucleic acid extraction | |
| CN212236267U (en) | High concentration is filter equipment for thick liquids | |
| CN215194448U (en) | Infusion apparatus capable of filtering and exhausting air | |
| CN221205598U (en) | Auxiliary extension device for ultrasonic endoscope puncture |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |