CN111549386A

CN111549386A - Vegetable and berry fruit fermentation product rayon fiber mask for moisturizing and resisting aging

Info

Publication number: CN111549386A
Application number: CN202010091719.6A
Authority: CN
Inventors: 林詠翔; 庄伟秀
Original assignee: TCI Co Ltd
Current assignee: TCI Co Ltd
Priority date: 2019-02-12
Filing date: 2020-02-12
Publication date: 2020-08-18
Also published as: TW202042777A

Abstract

The invention provides a vegetable berry fruit fermentation rayon fiber facial mask for moisturizing and aging resistance, which comprises a piece of rayon fiber cloth for increasing liquid carrying capacity and improving the stability of a cloth structure, and a vegetable berry fruit fermentation substance, and has the effects of increasing the water content and elasticity of skin, improving skin texture and smoothing wrinkles.

Description

Vegetable and berry fruit fermentation product rayon fiber mask for moisturizing and resisting aging

Technical Field

The invention relates to a facial mask, in particular to a moisture-preserving and anti-aging vegetable and fruit (or called vegetable and fruit) fermented rayon fiber facial mask.

Background

The maximum amount of raw materials used in the production of nonwoven fabrics made of artificial synthetic fibers at present is polypropylene (PP), Polyester (PET), Polyethylene (PE) and Nylon (Nylon) in sequence, which account for 96% of the total usage amount, and when these large amounts of nonwoven fabrics made of artificial synthetic fibers are used as waste materials, they cannot be decomposed by natural environment and cause great harm to the environment, so that the production of natural cellulose nonwoven fabrics made of wood pulp (pulp) as raw material by using solvent method is becoming mainstream, and the waste products are called green environmental protection fibers because they can be naturally degraded.

Rayon (Rayon), also known as Rayon, is a regenerated fiber produced from natural plant cellulose. Rayon has properties of easy dyeing, washing and acid and alkali resistance, and is often used for clothing, interior decoration (such as bedspreads and blankets), and also is frequently used in industry for manufacturing surgical instruments, nonwoven products, feminine hygiene products, tire cords and the like.

However, rayon cloth is generally loosened by swelling, and water absorption, tensile strength and mechanical strength are yet to be enhanced. In order to solve the above problems, those skilled in the art need to develop a novel method for preparing rayon cloth, and the tensile strength, mechanical strength, density and water absorption of the cloth, cloth film or facial mask prepared by the method need to be improved, so as to benefit the vast population who has the need.

Disclosure of Invention

In view of the problems faced by the prior art, an object of the present invention is to provide a rayon fiber comprising a plurality of supporting fibers obtained by alkalizing a fiber raw material, the fiber raw material being regenerated fibers made of plant cellulose; and nano-filler fibers embedded among the plurality of support fibers.

In an embodiment of the present invention, the plant cellulose is regenerated fiber made from cellulose raw materials such as wood pulp, wood chips or cotton chips, which is also called rayon.

In one embodiment of the present invention, the alkalization treatment is performed with sodium hydroxide.

In one embodiment of the present invention, the nano-filler fibers are elongated fiber structures made of bacterial cellulose by grinding, and the average diameter of the elongated fiber structures is less than 50 nanometers. Preferably, the bacterial cellulose is milled to produce nanofiller fibers having a diameter of 1-30 nm and a length of about 1-20 microns (preferably less than 2 microns).

In one embodiment of the present invention, the diameter of the nano-filler fiber is 1-4 nm.

In one embodiment of the present invention, the nano-filler fiber is an emerging industrial material, and is mainly applied to mask materials, high-strength materials (automobile parts, housing of household electrical appliances), high-functional materials (housing building materials, interior materials), tackifiers (foods, medical supplies), and special materials (special paper, filter materials).

Another object of the present invention is to provide a nano rayon fiber cloth comprising: a plurality of the foregoing rayon filaments are interlaced with each other to form a web.

Still another object of the present invention is to provide a facial mask for moisturizing and anti-aging, comprising: the foregoing nano rayon fiber cloth; and a fermented substance adsorbed in the nano rayon fiber cloth.

In an embodiment of the present invention, the fermented product is obtained by performing a fermentation reaction on a berry juice, wherein the berry juice is at least one selected from the group consisting of a blueberry juice, a cranberry juice, a white radish juice, a red bayberry juice, a mulberry juice, a grape juice, an apple juice, a sugarcane juice, a passion fruit juice, a pineapple juice, a lemon juice, a green cauliflower juice, a celery juice and a asparagus juice.

In one embodiment of the present invention, the fermentation reaction comprises: mixing vegetable juice and water at a weight ratio of 1:1-5, sterilizing at 50-100 deg.C for 0.5-1.5 hr; cooling to room temperature; sequentially implanting yeast (Saccharomyces cerevisiae BCRC20271) in the cooled vegetable and fruit juice, wherein the yeast accounts for 0.01-0.5 wt% of the whole vegetable and fruit juice, and fermenting for 1-3 days; inoculating lactobacillus (Streptococcus thermophilus TCI633) in the fruit and vegetable juice obtained in the previous step, wherein the lactobacillus accounts for 0.01-0.25 wt% of the fruit and vegetable juice, and fermenting for 1-5 days; concentrating under reduced pressure at 45-70 deg.C; finally, filtering with a 200-400 mesh (mesh) screen to obtain the fermented product.

It is still another object of the present invention to provide a method for preparing a nano rayon fiber cloth, comprising the steps of: (a) alkalizing the fiber raw material to obtain a plurality of supporting fibers; (b) fully mixing the nano filling fibers with a plurality of supporting fibers to obtain a fiber slurry mixture, wherein the nano filling fibers comprise bacterial cellulose, and the diameter of the nano filling fibers is 1-30 nanometers; (c) forming a plurality of rayon fibers from the fiber slurry mixture by a molding step, wherein the nano-filler fibers are embedded between the supporting fibers; and (d) through the weaving step, make a plurality of rayon fiber silk form the nanometer rayon fiber cloth, in order to increase the liquid and carry the sex and promote the stability of the cloth body structure.

In one embodiment of the present invention, in step (b), the ratio of the weight of the nano-filler fibers to the weight of the support fibers is 1:10 to 1: 20.

In one embodiment of the present invention, in step (a), the fiber raw material comprises one or a combination of wood pulp, wood chips, or cotton chips.

In one embodiment of the present invention, in step (a), the alkalization treatment is performed with sodium hydroxide.

Another object of the present invention is to provide a nano rayon fiber cloth, which is produced by the method as described above.

In one embodiment of the present invention, the stability of the body structure of the nano rayon fiber cloth is that the tensile strength (tensile strength) is greater than 20N.

In an embodiment of the present invention, the stability of the body structure of the nano rayon fiber cloth is that the stretch ratio is more than 120%.

Another object of the present invention is to provide a facial mask made using the nano rayon fiber cloth as described above, which has a liquid carrying capacity of more than 10 g of water content.

In summary, the nano rayon fiber cloth of the present invention has the effects of: skin feel is better than artificial fiber very much, is fit for developing the facial mask material of improvement, also can let nanometer filler fiber evenly distributed more easily when liquid, and can promote tensile strength, extensibility, mechanical strength, density and the water absorbing capacity of original rayon cloth, is an improvement cloth membrane material. Therefore, the facial mask made of the nano rayon fiber cloth can absorb more essence containing the vegetable and berry fermentation product, so that the facial mask can be more attached to skin when being applied to the skin and has better importing performance, and further the vegetable and berry fermentation product has the functions of oxidation resistance and skin beautifying, is more effectively imported to the skin and has the synergistic effect.

The following examples are presented to illustrate the present invention and are not intended to limit the scope of the present invention, which is not to be construed as limited thereby, and it is intended that all such modifications and variations that fall within the true spirit and scope of the present invention are deemed to be within the scope of the invention as defined by the appended claims.

Drawings

Fig. 1 is a schematic view of the structure of a nano rayon fiber cloth in one embodiment of the present invention;

fig. 2 is a graph comparing the density of a nano rayon fiber cloth according to an embodiment of the present invention;

fig. 3 is an electron microscope structural observation view of a nano rayon fiber cloth in an embodiment of the present invention;

fig. 4 is a data diagram of efficacy of the nano rayon fiber cloth in enhancing mechanical strength of the rayon cloth in an embodiment of the present invention;

fig. 5 is a graph of efficacy data of a nano rayon fiber cloth in increasing the stretching ratio of a rayon cloth in an embodiment of the present invention;

fig. 6 is a data diagram of efficacy of the nano rayon fiber cloth in promoting water absorption of the rayon cloth in an embodiment of the present invention;

FIG. 7 is a graph comparing Oxidative Radical Absorption Capacity (ORAC) of a fermented vegetable-berry product with a vegetable-berry juice product in accordance with an embodiment of the present invention;

FIG. 8 is a graph comparing the activity of a raspberry fruit ferment to a raspberry juice superoxide dismutase (SOD) in accordance with one embodiment of the present invention;

FIG. 9 is a graph comparing the collagen synthesis genes of skin fibroblasts with those of a fermented vegetable-berry fruit according to an embodiment of the present invention;

FIG. 10 is a graph comparing the synthesis of elastin in skin fibroblasts with a strawberry fermentation product in accordance with an embodiment of the present invention;

FIG. 11 is a graph of skin moisture content after 15 minutes of using a raspberry fruit ferment in accordance with one embodiment of the present invention;

FIG. 12 is a graph showing the results of a skin elasticity test conducted after 15 minutes using a strawberry and vegetable ferment in accordance with an embodiment of the present invention;

FIG. 13 is a graph showing the results of a skin texture test conducted after 15 minutes using a raspberry fruit ferment in accordance with one embodiment of the present invention.

FIG. 14 is a graph of skin wrinkle test results after 15 minutes using a raspberry fruit ferment in accordance with one embodiment of the present invention.

Wherein, the reference numbers:

supporting fiber 11

Nano-filled fibers 12

Rayon staple 13

Nano rayon fiber cloth 14

Detailed Description

In the following description, for purposes of explanation, numerous implementation details are set forth in order to provide a thorough understanding of the various embodiments of the invention. However, these implementation details should not be used to limit the invention. That is, in some embodiments of the invention, such implementation details are not necessary. As used herein, the numerical values are approximations and all numerical data are reported to be within the 20 percent range, preferably within the 10 percent range, and most preferably within the 5 percent range.

In this document, unless the context requires otherwise, the word "a" and "an" may mean "one" or "more". It will be further understood that the terms "comprises," "comprising," "includes" and/or "including," when used herein, specify the presence of stated features, regions, integers, steps, operations, elements, and/or components, but do not preclude the presence or addition of one or more other features, regions, integers, steps, operations, elements, components, and/or groups thereof.

From the foregoing, it is known that general rayon cloth is loosened by swelling, and water absorption, tensile strength and mechanical strength are yet to be enhanced. In view of this, the present invention provides a nano rayon fiber cloth having good tensile strength, mechanical strength, density and water absorption. The essence containing the raspberry fruit ferment (hereinafter referred to as "the raspberry fruit ferment essence") provided by the invention has good moisturizing and anti-aging functions, and further becomes the nano rayon fiber mask containing the raspberry fruit ferment essence. The mask can adsorb more essence of the raspberry and fruit fermentation product with the moisturizing and anti-aging functions, and can also introduce the essence of the raspberry and fruit fermentation product into skin better by utilizing the good tensile strength, mechanical strength and density of the mask, so that the skin has better moisturizing and anti-aging capabilities.

The preparation method and the efficacy test of the nano rayon fiber cloth and the vegetable berry fruit fermentation essence nano rayon fiber mask provided by the invention are explained in detail below, so as to confirm that the mask has good absorption force, tensile strength, mechanical strength and density, and cells can absorb essence more easily; further proves that the raspberry and fruit fermented essence provided by the invention has the functions of moisturizing and resisting aging.

Example 1 preparation of supporting fiber, preparation of Nanofilled fiber and structural stability test of cloth body

Please refer to fig. 1, which is a schematic structural diagram of a nano rayon fiber cloth 14. First, a supporting fiber 11 is prepared, wherein the fiber material of the supporting fiber 11 is regenerated fiber made of cellulose material such as wood pulp, wood chips, or cotton chips, which is also called rayon. The fiber material is alkalized with sodium hydroxide to obtain an alkalized and homogeneous support fiber 11, which may also be referred to as a support rayon fiber in the present invention. Next, the nano-filler fibers 12 are prepared, wherein the nano-filler fibers 12 are selected from bacterial cellulose, and are milled to form nano-filler fibers 12 having a diameter of about 1-30 nm and a length of about 1-20 microns, preferably less than 2 microns.

The nano-filler fibers 12 are added to the alkalized support fibers 11 and mixed thoroughly to obtain a fiber slurry mixture in which the weight ratio of the nano-filler fibers 12 to the support fibers 11 is 1:10 to 1: 20. Then, the step of forming the liquid fiber slurry mixture is carried out: the mixture of the fiber pulp is ejected through a spinneret and coagulated into filaments in an aqueous solution of sulfuric acid to produce the rayon filaments 13.

Referring to fig. 1, the supporting fibers 11 are not ground and are longer fibers, so that after being ejected from the spinneret, the plurality of long supporting fibers 11 form a single rayon filament, wherein gaps are formed among the long supporting fibers 11, and the nano-filler fibers 12 are embedded in the gaps among the plurality of long supporting fibers 11, i.e., at least a portion of the nano-filler fibers 12 are located in the single rayon filament, so that a denser rayon filament is formed. Subsequently, the rayon filaments 13 can be further loosened into smaller bundles and blocks by, for example, a cotton opener, and then uniformly put into, for example, a carding machine to be made into a web, through a spinning step. Thereafter, the web is stacked to a desired thickness of about 0.01 to 0.5 cm and then placed into a needle punch, for example, to be needle-punch formed into a sheet to form the nano rayon fiber sheet 14.

The process for producing the above nano rayon fiber cloth 14 can be entrusted to the chemical fiber CORPORATION of Taiwan (formosaccheials & fabric CORPORATION).

In another embodiment of the present invention, the nano-filler fibers have a diameter in the range of 1-30 nanometers and a length of about 1-20 microns, preferably less than 2 microns.

In another embodiment of the present invention, the nano-filler fibers have a diameter falling within the range of 1-4 nanometers.

In the embodiment of the present invention, the filling fiber is preferably made of bacterial cellulose (also called biological fiber).

Next, the nano rayon fiber cloth 14 was subjected to a cloth structural stability (density) test and compared with a general rayon cloth film (no filled fiber). The "general rayon film" (or "general rayon cloth") according to the present invention is manufactured in substantially the same manner as the nano rayon fiber cloth 14, except that the nano filler fiber 12 is not added. The comparison results are shown in FIG. 2. Fig. 2 is a graph comparing the density of the nano rayon fiber cloth. As can be seen from fig. 2, compared with a general rayon cloth film, a nano rayon fiber cloth can maintain an original structure in water, and the general rayon cloth film is loosened by swelling. The result of this embodiment shows that nano rayon fiber cloth is more dense than a general rayon fiber film in appearance, and can maintain a stable and compact structure of rayon fiber when placed in liquid, so that the density of rayon fiber cloth can be effectively improved by the nano rayon fiber cloth of the present invention.

In one embodiment of the present invention, when the ratio of the nano-filler fibers to the supporting fibers is increased, the overall smooth touch of the cloth body is also increased. Such as nano-filled fibers in the cloth or in the slurry prior to forming the cloth: when the weight ratio of the supporting fibers was adjusted from 1:20 to 1:10, it was found that when the nano-filler fibers: when the weight ratio of the supporting fiber is 1:10, the touch feeling is better than that of 1: 20.

Example 2 electron microscope structural observation of nano rayon fiber cloth

One embodiment of the present invention cuts a general rayon film and the nano rayon fiber cloth of embodiment 1 into test sheets having a length of 1 cm and a width of 1 cm, respectively, and uses carbon glue (available from TED PELLA,

conductive silver paint,30g #16062) is adhered to the metalAnd (7) carrying a platform. Next, the stage was placed in a Hitachi tablet Microscope (TM-1000) desktop electron Microscope, and the appearance of the fibers was observed under vacuum at different magnifications. Fig. 3 is an electron microscope structural observation view of the nano rayon fiber cloth. As can be seen from fig. 3, observing the structural configuration of the nano rayon fiber film with a 6000-fold electron microscope, it can be found that nano-filled fibers are embedded in the fiber bundle of rayon (as indicated by arrows), and the rayon fiber film is generally absent.

Example 3 mechanical strength and Material ductility test of Nano rayon fiber cloth

In this embodiment, the mechanical properties of the nano rayon fiber cloth and the general rayon cloth in example 1 are tested for the structural stability of the cloth body, including the mechanical strength (i.e., tensile strength) and elongation (elongation). First, a film-formed nano rayon fiber cloth and a general rayon cloth are stacked in three layers, and are cut into tensile test pieces each having a length of 5 cm and a width of 1 cm, and the thickness of each tensile test piece is measured. And then, a Shimadzu EZ Tester 500N strength Tester is used for testing the tensile strength of the film, the distance between an upper clamp and a lower clamp is 1.5 cm, the test is carried out at the tensile rate of 10mm/min, and two sides of the test are clamped on a clamp. After that, the stress-strain curve and the maximum load value of the stretching process are recorded, and the maximum stress of the nano rayon fiber cloth and the general rayon cloth under the same basis weight condition is compared. When the fiber is stretched to complete fracture, the maximum extending length of the fiber is recorded, and the tensile strength and the elongation can be obtained through a stress-strain diagram so as to compare the physical strength of each material. The results of this example are shown in FIGS. 4 and 5.

Fig. 4 is a data diagram of the efficacy of the nano rayon fiber cloth in promoting the mechanical strength of the rayon cloth. As can be seen from fig. 4, the maximum stress of the nano rayon fiber cloth is increased by 90% compared to that of general rayon cloth, confirming that the nano rayon fiber cloth has higher mechanical strength. Fig. 5 is a data diagram of the efficacy of nano rayon fiber cloth in increasing the stretch ratio of rayon cloth. As can be seen from fig. 5, the stretching ratio of the nano rayon fiber cloth is improved by 20% compared to that of the general rayon cloth, confirming that the nano rayon fiber cloth has excellent extensibility.

Example 4 material liquid loading test of nano rayon fiber cloth

First, a general rayon film and the nano rayon fiber cloth in example 1 were cut into test sheets 10 cm in length and 10 cm in width, respectively, and the weight was recorded, and then both the test sheets were immersed in 50 ml of pure water for 30 minutes. The results of this example are shown in FIG. 6. Fig. 6 is a data diagram of efficacy of the nano rayon fiber cloth in promoting water absorption of the rayon cloth. As can be seen from fig. 6, the gap of a rayon fiber filament embedded with nano filler fiber can be filled with nano filler fiber, so that the water absorption of a nano rayon fiber cloth formed by a rayon fiber filament embedded with nano filler fiber is 1.9 times that of a general rayon cloth. The result of this embodiment shows that the nano rayon fiber cloth can effectively promote the water absorption of the rayon cloth.

In one embodiment of the present invention, when the ratio of the nano-filler fibers to the support fibers is increased, the moisture absorption force is also increased. Such as nano-filled fibers in the cloth or in the slurry prior to forming the cloth: when the weight ratio of the supporting fiber is adjusted from 1:20 to 1:10, the moisture absorption capacity is increased. This is because the nano rayon fiber cloth has embedded nano filler fibers, and thus the entire surface area is increased, so that the rayon cloth has better moisture absorption force than the general rayon cloth.

In conclusion, the skin feeling of the nano rayon fiber cloth of the invention is much better than that of common rayon cloth and other artificial fibers, is suitable for developing improved facial mask materials, can more easily and uniformly distribute nano filler fibers in a liquid state, can effectively improve the tensile strength, the extensibility, the mechanical strength, the density and the water absorption of original rayon cloth, and is an improved cloth film material.

Example 5 preparation of a vegetable berry fruit ferment rayon fiber mask

According to an embodiment of the invention, the preparation process of the raspberry and fruit fermented product comprises the following steps:

1. the method comprises mixing raspberry juice (MX-AC130-FC, available from Yangya marketing Co., Ltd.) and raspberry juice (MX-AC134-FL, available from Yangya marketing Co., Ltd.) at a weight ratio of 1:1 to obtain a raspberry juice (or called vegetable juice), mixing the raspberry juice with water at a ratio of 1:1-5, sterilizing at 50-100 deg.C for 0.5-1.5 hr;

2. cooling the vegetable berry juice obtained in the step (1) to room temperature (25 +/-5 ℃) for subsequent fermentation;

3. implanting yeast (Saccharomyces cerevisiae BCRC20271) into the fruit juice obtained in step 2 (after implantation, the yeast accounts for 0.01-0.5 wt% of the whole fruit juice), and fermenting for 1-3 days;

4. inoculating lactobacillus (Streptococcus thermophilus TCI633) into the fruit juice obtained in step 3 (after the inoculation, the lactobacillus accounts for 0.01-0.25 wt% of the whole fruit juice), and fermenting for 1-5 days;

5. concentrating under reduced pressure at 45-70 deg.C;

6. filtering with 200-mesh 400 mesh sieve to obtain fermented product, wherein the total polyphenol content of the fermented product is above 40 microgram/mL (microgram/mL), and the SOD content is above 700U/mL.

In some embodiments, the berry juice (or called vegetable juice) used in the aforementioned step 1 may be at least one selected from the group consisting of blueberry juice, cranberry juice, white radish juice, red radish juice, waxberry juice, mulberry juice, grape juice, apple juice, sugarcane juice, passion fruit juice, pineapple juice, lemon juice, green cauliflower juice, celery juice, and asparagus juice.

Example 6 analysis of the oxidative free radical absorption Capacity (ORAC) of fermented and fruit juice of Rubus serrulata

In one embodiment of the present invention, two test samples with equal weight are prepared, wherein one is the "vegetable berry juice" obtained in step 1 of example 5, and the other is the "vegetable berry fermented product" obtained by fermentation in steps 1-6 of example 5; the "vegetable berry juice" and "vegetable berry fruit fermented product" were sent to Tendem technical consultant GmbH and analyzed by ORAC, and the results are shown in FIG. 7. As can be seen from fig. 7, the oxidation radical absorption capacity of the fermented product of rubus suavissimus provided by the present invention is 1.4 times of that of the rubus suavissimus fruit juice, and it can be seen that the original oxidation radical absorption capacity can be improved after the fermented product of rubus suavissimus fruit juice is fermented into the fermented product of rubus suavissimus fruit juice.

Example 7 analysis of the SOD Activity of fermented product and fruit juice of Rubus suavissimus L

One embodiment of the present invention is a test experiment of the activity efficacy of superoxide dismutase (SOD) in the fermented product of rubus suavissimus, wherein the test is performed with reference to the standard of determination of the activity of superoxide dismutase in GB/T5009.171-2003 health food, and the rubus suavissimus juice described in example 6 is compared with the fermented product of rubus suavissimus, wherein the SOD activity is quantified based on the efficiency of the analyte for inhibiting the self-oxidation reaction of Pyrogallol. SOD, an enzyme that catalyzes superoxide through disproportionation and converts superoxide into oxygen and hydrogen peroxide, is widely found in various animals, plants, and microorganisms, and is an important antioxidant in the body to protect cells exposed to oxygen.

First, a solution a and a solution B were prepared for measurement, wherein the solution a was prepared by dissolving 1.2114 g of Tris (hydroxymethyl) aminomethane (Tris) and 37.2 mg (mg) of ethylenediaminetetraacetic acid (EDTA-2Na) in 62.4 mL (mL) of a 0.1mole/L hydrochloric acid (HCl) solution and quantifying to 100 mL with pure water after dissolution, and the solution B was prepared by dissolving 56.7 mg of pyrogallol (pyrogallol) in a small amount of hydrochloric acid having a concentration of 10mmole/L and quantifying to 100 mL with pure water after dissolution.

Treating a sample to be tested, wherein the solid sample is prepared by the following method: weighing 1 g of solid sample, placing the solid sample in a mortar, adding 9 ml of pure water, grinding and dissolving, transferring all liquid into a centrifugal tube, washing the mortar with a small amount of pure water, then adding the residual liquid into the centrifugal tube, quantifying the residual liquid with the pure water until the total volume is 10 ml, centrifuging the liquid at 4000rpm for 15 minutes, and taking the supernatant for determination. The liquid samples were prepared as follows: the clarified liquid sample can be directly measured as a stock solution; the turbid liquid sample was centrifuged at 4000rpm for 5 minutes and the supernatant was taken for assay. The liquid sample can be diluted properly with pure water and finally multiplied by the dilution factor to calculate the original concentration.

Next, referring to the following table one, a blank measurement of the control group was performed: 2.35 ml of the solution A and 2 ml of the solution A are added into a centrifuge tube in sequenceAdding 0.15 ml of solution B after shaking and mixing the pure water uniformly, quickly and lightly shaking and mixing the solution B after adding the solution B, immediately pouring the mixture into a colorimetric tube to measure the light absorption value of the mixture at the wavelength of 325 nanometers, detecting the light absorption value at the wavelength of 325 nanometers again after reacting for 1 minute, wherein the difference value of the two values is delta A₃₂₅(ii) a And the sample to be tested is respectively added with 2.35 ml of solution A, 1.8 ml of pure water, 0.2 ml of sample to be tested (vegetable berry juice and vegetable berry fermented product treated in the manner described in the previous paragraph) and 0.15 ml of solution B in sequence in a centrifuge tube, after the solution B is added and mixed, the mixture is quickly and lightly shaken and uniformly mixed, and immediately poured into a colorimetric tube to test the light absorption value at the wavelength of 325 nanometers, after the reaction is carried out for 1 minute, the light absorption value at the wavelength of 325 nanometers is tested again, and the difference value of the two values is delta A'₃₂₅。

Table I, SOD activity measuring table

Reagent	Blank space	Sample (I)
			Solution A	2.35 ml	2.35 ml
Pure water	2 ml of	1.8 ml
			Sample to be tested	－	0.2 ml
Solution B	0.15 ml	0.15 ml

Finally, the SOD activity in the vegetable-berry fruit juice and the vegetable-berry fruit fermentation product is calculated according to the following formula, wherein V is 4.5, and D is 0.2.

ΔA₃₂₅: auto-oxidation speed of pyrogallol

ΔA′₃₂₅: sample inhibition of pyrogallol autoxidation speed

V: volume of sample

D: dilution factor of sample

The test result of the activity of the superoxide dismutase in the blackberry fruit fermented product is shown in figure 8, and the superoxide dismutase activity in the blackberry fruit fermented product is 1.7 times that of the blackberry fruit juice according to figure 8. The results show that the vegetable, berry and fruit fermented product can improve the activity of superoxide dismutase so as to achieve the purpose of enhancing the health-care antioxidant effect.

Example 8 analysis of genes for the production of collagen and elastin by skin fibroblasts from the fermentation of berries of vegetable

The invention uses human skin fibroblast (CCD-966sk) to carry out cell experiments on the vegetable and berry fruit fermentation product. The human dermal fibroblasts are purchased from the American type culture Collection under the number CRL-1881. The cells were cultured in MEM (Minimum essential medium, available from Gibco, USA 12100-.

In this example, the human dermal fibroblasts were cultured in 24-well plates containing the MEM medium, and 2X10 cells were implanted in each well⁴The cells were cultured at 37 ℃ for 24 hours. Then will beCells were divided into two groups: the first group is a Control group (Control)); the second group was the experimental group to which the fermented product of rubus suavissimus of example 5 was added to give a final concentration of 1 mg/mL. The following procedure was followed after incubation at 37 ℃ for 24 hours.

The expression level of genes (target genes) involved in the synthesis of collagen and elastin in cells is measured based on quantitative polymerase chain reaction (qPCR), and the procedure is briefly described below. RNA was isolated from the cells using an RNA Extraction Kit (RNA Extraction Kit; Geneaid) according to the manufacturer's instructions and reverse transcriptase was used at 37 deg.C

III Reverse Transcriptase 2000ng of RNA was Reverse transcribed to cDNA (Invitrogen). Subsequently, the cDNA was subjected to qPCR using a qPCR Kit (KAPA CYBR FAST qPCR Kit (2X); KAPA Biosystems) in a PCR reactor (Step One plus real-Time PCR system; Applied Biosystems) using a primer pair of the target gene and a Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) gene as an internal control (Table II) to obtain a melting curve (melting curve).

List two, primer sequence list

Finally, use 2^-ΔΔCTThe method determines the relative expression of the target gene. The method uses the cycle threshold (C) of GAPDH gene_T) The cycle threshold of the reference gene as an internal control was calculated as the relative fold change according to the following formula:

ΔC_Tc of target gene in experimental group or control group_TC of internal control_T

Δ Δ CT ═ Δ C of experimental groups_TΔ C of control groups_T

Multiple change 2^{- Δ Δ CT mean value}

Statistical analysis was performed by calculating the standard deviation of relative expression of each gene using the STDEV function in Excel software and calculating the statistical difference using the single-tailed student t test (TTEST).

In this example, expression amounts of COL1A1 gene of alpha 1-type I collagen (collagen, type I, alpha 1), COL1A2 gene of alpha 2-type I collagen (collagen, type I, alpha 2) and ELN gene of Elastin (Elastin) in control group and experimental group (fermented product of berry fruit) were measured, to prove whether the fermented vegetable berry fruit provided by the present invention has the function of promoting the generation and synthesis of collagen and elastin, please refer to fig. 9 and 10, as a result, the expression levels of COL1a1 and COL1a2 genes can be found to be 1.22 and 1.26 times of those of the control group respectively (p is less than 0.05) in fig. 9, and the expression level of ELN can be also seen to be 1.29 times of that of the control group (p is less than 0.001) in fig. 10, therefore, the vegetable-berry fruit fermented product provided by the invention can improve the expression quantity of collagen and elastin genes, and further improve the biosynthesis of collagen and elastin.

Example 9 vegetable and berry fruit ferment mask human efficacy test

The experimental samples of this example are a raspberry fruit ferment mask and a placebo mask, wherein the fabrics used by the raspberry fruit ferment mask and the placebo mask are the same, and the liquid substances contained in the raspberry fruit ferment mask and the placebo mask are respectively shown in table three in the component ratio:

ingredient ratio of liquid substance contained in surface pack

	Fruit and vegetable fermented mask (%)	Placebo mask (%)
			Example 5 Raspberry fruit fermentate	80	－
Antioxidant agent	0.6	0.6
			Moisture-retaining agent	5	5
Thickening agent	0.3	0.3
			Solubilizer	0.6	0.6
pH balancing agent	0.1	0.1
			Water (W)	13.4	93.4

Wherein, for example, the antioxidant can be menthone (Hydroxyacetophenone); the humectant can be 1, 3-butanediol; the thickener can be Xanthan gum (Xanthan gum) or/and Acrylate/C10-30 alkanol Acrylate Crosspolymer (Acrylates/C10-30 Alkyl Acrylate crosslinker), and the solubilizer can be 1,2-Hexanediol (1, 2-Hexanediol); the pH balancing agent may be Triethanolamine (Triethanolamine).

In the experiment of this example, 8 subjects each applied the above-mentioned fermented vegetable and fruit product mask and placebo mask on the left and right half faces, respectively, and after 15 minutes, the skin was removed to promote absorption by applying a slight massage to the abdomen, and before and after use, the skin texture Analysis System (VISIA complex Analysis System) (available from Canfield scientific, USA) was used for the test, and the test items were: the skin moisture content, elasticity, texture state and wrinkle state, where "texture state detection" is a value obtained by calculating the smoothness of the skin with respect to the difference in height of the cheek region using an instrument, and "wrinkle state detection" is a value obtained by evaluating the depth of wrinkles around the eyes together with the number thereof using an instrument, and the results are shown in fig. 11 to 14.

From fig. 11, it can be seen that the skin moisture content of the test subjects was significantly increased by 13.7% (p is 0.01) compared to the pre-use mask, whereas the placebo mask increased only by 3.2% without significance; from fig. 12, it can be seen that the skin elasticity of the subjects after using the raspberry fruit ferment mask of the present invention was increased by 4.4% compared to before use, while the placebo mask was only 0.9% changed; from fig. 13, it can be seen that the skin texture state of the subjects was improved by 2.9% compared to that before the use after the administration of the raspberry fruit ferment mask of the present invention, while there was no significant difference between the subjects after the administration of the placebo mask and before the use; finally, it can be seen from fig. 14 that the skin wrinkle status of the subjects was greatly improved by 50% (p <0.05) compared to the subjects before use, while the subjects were improved by only 29.4% with the placebo mask, after use of the raspberry fruit ferment mask of the present invention.

According to the invention, the raspberry and fruit ferment mask can effectively increase the moisture content and elasticity of skin, and has the effects of improving texture and smoothing wrinkles and beautifying skin.

It can be known from the foregoing embodiments or examples of the present invention that the nano rayon fiber cloth provided by the present invention is an improved mask material, has a skin feel much better than that of artificial fibers, and can improve the tensile strength, extensibility, mechanical strength, density and water absorption of rayon cloth; in addition, the raspberry and fruit ferment provided by the invention can improve the absorption capacity of cells for oxidizing free radicals and the antioxidation effect, and can also improve the biosynthesis of collagen and elastin, and human body efficacy tests further prove that the raspberry and fruit ferment mask can effectively increase the water content and elasticity of skin, and has the effects of improving textures and smoothing wrinkles and beautifying muscles.

The foregoing is by way of example only, and not limiting. Any equivalent modifications or variations without departing from the spirit and scope of the present invention should be included in the scope defined in the appended claims.

Sequence listing

<110> Dajiang biomedical corporation Ltd

<120> a vegetable berry fruit ferment rayon fiber facial mask for moisturizing and aging resistance

<150>US62/804,228、US 62/871,216

<151>2019-02-12、2019-07-08

<160>6

<210>1

<211>21

<212>DNA

<213> Artificial sequence

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Claims

1. A rayon filament characterized by comprising:

a plurality of supporting fibers obtained by alkalizing a fiber raw material, which is regenerated fibers made of plant cellulose; and

and the nano filling fibers are embedded among the plurality of supporting fibers.

2. The rayon fiber filament according to claim 1, characterized in that said plant cellulose is derived from wood pulp, wood chips or cotton chips.

3. The rayon fiber filament according to claim 1, characterized in that said alkalizing treatment is carried out with sodium hydroxide.

4. The rayon filament according to claim 1, characterized in that said nano-filler fiber is made of bacterial cellulose by grinding.

5. The rayon fiber filament according to claim 1 or 4, characterized in that said nano-filler fiber has a diameter of 1 to 30 nanometers.

6. The rayon fiber filament according to claim 1 or 4, characterized in that said nano-filler fiber has a diameter of 1 to 4 nanometers.

7. A nano rayon fiber cloth characterized by comprising:

a plurality of rayon fiber filaments according to any one of claims 1 to 6, interlaced with one another into a web.

8. A facial mask for moisturizing and anti-aging, comprising:

the nano rayon fiber cloth according to claim 7; and

and the ferment adsorbed in the nano rayon fiber cloth.

9. The mask of claim 8, wherein the fermented product is obtained by fermentation of a vegetable juice, and the vegetable juice is at least one selected from the group consisting of blueberry juice, cranberry juice, white radish juice, red bayberry juice, mulberry juice, grape juice, apple juice, sugarcane juice, passion fruit juice, pineapple juice, lemon juice, broccoli juice, celery juice, and asparagus juice.

10. The mask of claim 9, wherein the fermentation reaction comprises:

mixing the vegetable juice with water at a weight ratio of 1:1-5 to obtain a mixed solution of the vegetable juice and the water, and sterilizing and extracting at 50-100 ℃ for 0.5-1.5 hours;

cooling to room temperature;

implanting yeast (Saccharomyces cerevisiae BCRC20271) in the cooled mixed solution of the vegetable and fruit juice and water, wherein the yeast accounts for 0.01-0.5 wt% of the whole mixed solution of the vegetable and fruit juice and then fermenting for 1-3 days;

implanting lactobacillus (Streptococcus thermophilus TCI633) in the mixed solution of the vegetable and fruit juice and water obtained in the previous step, wherein the lactobacillus accounts for 0.01-0.25 wt% of the whole mixed solution of the vegetable and fruit juice and water, and fermenting for 1-5 days;

concentrating under reduced pressure at 45-70 deg.C; and

filtering with 200-400 mesh sieve to obtain the fermented product.

11. A method for preparing a nano rayon fiber cloth, characterized by comprising the steps of:

(a) performing alkalization treatment on a fiber raw material to obtain a plurality of supporting fibers, wherein the fiber raw material is regenerated fibers made of plant cellulose;

(b) fully mixing nano filling fibers with the plurality of supporting fibers to obtain a fiber slurry mixture, wherein the nano filling fibers comprise bacterial cellulose, and the diameter of the nano filling fibers is 1-30 nanometers;

(c) forming the fiber slurry mixture into a plurality of rayon fibers by a forming step, wherein the nano-filler fibers are embedded between the plurality of supporting fibers; and

(d) the plurality of rayon filaments are formed into the nano rayon fiber cloth through the weaving step.

12. The method of claim 11, wherein in step (b), the ratio of the weight of the nano-filler fibers to the weight of the plurality of support fibers is from 1:10 to 1: 20.

13. The method of claim 11, wherein in step (a), the fibrous feedstock comprises one or a combination of wood pulp, wood chips, or cotton chips.

14. The method according to claim 11, wherein in step (a), the alkalization treatment is performed with sodium hydroxide.

15. The method of claim 11, wherein the diameter of the nano-filler fiber is 1-4 nanometers.