CN111549049B - 一种产核黄素的重组海藻希瓦氏菌及在产电中的应用 - Google Patents

一种产核黄素的重组海藻希瓦氏菌及在产电中的应用 Download PDF

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CN111549049B
CN111549049B CN202010478390.9A CN202010478390A CN111549049B CN 111549049 B CN111549049 B CN 111549049B CN 202010478390 A CN202010478390 A CN 202010478390A CN 111549049 B CN111549049 B CN 111549049B
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宋浩
陈媛媛
李锋
曹英秀
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Abstract

本发明公开了一种产核黄素的重组海藻希瓦氏菌及在产电中的应用，该重组菌，用下述方法构建：(1)将黄素合成途径中的五个目的基因ribA，ribC，ribD，ribE，ribH连接到载体pyydt上，得到重组质粒R5；(2)将重组质粒R5，转入海藻希瓦氏Shewanella algae scs‑1 CGMCCNo.18696菌株，得到一种产核黄素的重组海藻希瓦氏菌scs‑R5。本发明的重组菌，电子传递体核黄素的产量高，从而增加其产电能力。

Description

一种产核黄素的重组海藻希瓦氏菌及在产电中的应用
技术领域
本发明属于生物能源技术领域，具体涉及一株产核黄素的重组海藻希瓦氏菌及在产电中的应用。
背景技术
微生物燃料电池是一种利用微生物作为生物催化剂，将有机物中的化学能转化成电能，是一种清洁可持续的能源形式。产电菌在电池阳极氧化分解有机物产生电子和质子，电子通过阳极经过外电路到达阴极形成电流，质子经过质子交换膜从阳极到达阴极，与阴极的电子受体发生还原反应，从而形成一个完整的闭合回路。阳极的电子传递是限制微生物产电能力的一个主要方面，目前研究证实的电子传递机制有两种：直接传递和间接传递，直接传递是指产电微生物通过导电细胞色素或者导电纳米导线直接与阳极接触将电子传递给阳极；间接传递，指产电微生物自身分泌的电子传递体，通过扩散运动将所携带的电子传递给阳极的过程。海藻希瓦氏菌在培养的过程中很容易附着在电极上形成较厚的生物膜，非常有利于其直接电子传递。海藻希瓦氏菌可以产生少量的黄素类物质作为电子传递体，但其自身产量较低。此外，通过在电池中外源添加核黄素溶液可以大幅度提高其产电能力，但是外源添加核黄素成本较高，不利于其在后续的工业化应用。因此选择以合成生物学的方法来异源表达核黄素作为电子受体，是增强海藻希瓦氏菌产电能力的重要方法。
发明内容
本发明的目的是克服现有技术的不足，提供一种产核黄素的重组海藻希瓦氏菌。
本发明的第二个目的是一种产核黄素的重组海藻希瓦氏菌在产电中的应用。
本发明的技术方案概述如下：
一种产核黄素的重组海藻希瓦氏菌，用下述方法构建：
(1)将黄素合成途径中的五个目的基因ribA，ribC，ribD，ribE，ribH连接到载体pyydt上，得到重组质粒R5，其质粒图谱如图1所示；
所述基因ribA的核苷酸序列用SEQ ID NO.1所示；
所述基因ribC的核苷酸序列用SEQ ID NO.2所示；
所述基因ribD的核苷酸序列用SEQ ID NO.3所示；
所述基因ribE的核苷酸序列用SEQ ID NO.4所示；
所述基因ribH的核苷酸序列用SEQ ID NO.5所示；
所述载体pyydt的核苷酸序列用SEQ ID NO.6所示
所述重组质粒R5的核苷酸序列用SEQ ID NO.7所示；
(2)将重组质粒R5，转入海藻希瓦氏Shewanella algae scs-1CGMCCNo.18696菌株，得到一种产核黄素的重组海藻希瓦氏菌scs-R5。
上述一种产核黄素的重组海藻希瓦氏菌在产电中的应用。
本发明的优点
本发明的一种产核黄素的重组海藻希瓦氏菌scs-R5，其电子传递体核黄素的产量高，从而增加其产电能力。与野生型的海藻希瓦氏菌相比，重组菌株海藻希瓦氏菌scs-R5能过表达核黄素，显著提高产电能力，在20mM的乳酸作为电子供体时，其最大输出电压为590±10mV，其最大功率密度为1200±15mW/m2。结合阳极材料后其产电能力得到了进一步的提升，在20mM的乳酸作为电子供体时，其最大输出电压为640±20mV，其最大功率密度为2200±50mW/m2。
附图说明
图1为重组质粒R5质粒图谱。
图2为重组海藻希瓦氏菌scs-R5在不同浓度诱导剂的条件下的黄素类物质表达量。
图3菌株以碳布为电极的电化学表征；其中
(A)为重组海藻希瓦氏菌scs-R5与野生型海藻希瓦氏菌scs-1的电压-时间图。
(B)为重组海藻希瓦氏菌scs-R5与野生型海藻希瓦氏菌scs-1的伏安循环曲线图。
(C)为重组海藻希瓦氏菌scs-R5与野生型海藻希瓦氏菌scs-1的功率密度曲线图。
(D)为重组海藻希瓦氏菌scs-R5与野生型海藻希瓦氏菌scs-1的极化曲线图。
图4为重组海藻希瓦氏菌scs-R5电极的共聚焦激光扫描显微镜图。
(A)普通碳布(对照)用于重组海藻希瓦氏菌scs-R5的阳极三维图。
(B)碳纳米管导电棉复合电极用于重组海藻希瓦氏菌scs-R5的阳极三维图。
图5为重组海藻希瓦氏菌scs-R5在应用碳纳米管导电棉复合电极时的电化学表征。
(A)为重组海藻希瓦氏菌scs-R5应用碳纳米管导电棉复合电极的电压图。
(B)伏安循环曲线图。
(C)功率密度曲线图。
(D)极化曲线图。
具体实施方氏
下面通过具体实施例对本发明作进一步的说明。
海藻希瓦氏菌(Shewanella algae)scs-1已于2019年10月16日保藏于由中国微生物菌种保藏管理委员会普通微生物中心，地址，北京市朝阳区北辰西路1号院3号中国科学院微生物研究所，保藏编号：CGMCC No.18696。
实施例1
一种产核黄素的重组海藻希瓦氏菌的构建与表达：
将黄素合成途径中的五个目的基因ribA，ribC，ribD，ribE，ribH与连接到载体pyydt上，得到重组质粒R5，其质粒图谱如图1所示。
所述基因ribA的核苷酸序列用SEQ ID NO.1所示；
所述基因ribC的核苷酸序列用SEQ ID NO.2所示；
所述基因ribD的核苷酸序列用SEQ ID NO.3所示；
所述基因ribE的核苷酸序列用SEQ ID NO.4所示；
所述基因ribH的核苷酸序列用SEQ ID NO.5所示；
所述载体pyydt的核苷酸序列用SEQ ID NO.6所示
所述载重组质粒R5的核苷酸序列用SEQ ID NO.7所示；
将得到的重组质粒R5，转入大肠杆菌WM3064感受态细胞(商业菌株)中，得到WM3064-R5，然后将WM3064-R5与保藏编号为CGMCCNo.18696的菌株海藻希瓦氏(Shewanellaalgae)scs-1结合转移，将重组质粒转入海藻希瓦氏(Shewanella algae)scs-1中，通过筛选得到一种产核黄素的重组海藻希瓦氏菌scs-R5(以下简称重组菌scs-R5)，将其保存在-80℃冰箱。
将上述得到的重组菌scs-R5，接种于含有卡那霉素抗性的LB液体培养基(卡那霉素的浓度25μg/mL)中，在转速为200r/min，温度为30℃的摇床中培养过夜，得到一级种子液，将得到的一级种子液转接到含有卡那霉素抗性的LB液体培养基(卡那霉素的浓度25μg/mL)中，加入诱导剂异丙基-β-D-硫代半乳糖苷(IPTG)，其终浓度分别为1mM，0.75mM，0.5mM，0.25mM，0mM，在转速为200r/min，温度为30℃的摇床中过夜培养，得到发酵液。
将上述得到的发酵液利用高效液相色谱测量其黄素产量，得到在不同浓度诱导剂下的黄素产量，结果如图2所示，从图中可以看出重组菌scs-R5最优的诱导剂浓度为0.5mM，黄素浓度达到72±4mg/L，与野生型海藻希瓦氏scs-1(黄素浓度15.5±4mg/L)相比较，重组菌scs-R5引入异源黄素表达途径后，其黄素的表达量是野生型的4.6倍左右。黄素合成量的增加有利于以电子传递体导电的间接电子传递，弥补了野生型海藻希瓦氏菌scs-1电子传递体的不足。
实施例2
重组菌scs-R5在产电中的应用
将构建好的重组菌scs-R5与野生型菌株scs-1划线活化，将重组菌scs-R5接种于含有卡那霉素(浓度25μg/mL)的LB液体培养基中，得到一级种子液。
取一级种子液20mL转接到400mL阳极液中(卡那霉素浓度25μg/mL)，加入诱导剂IPTG终浓度为0.5mM，过夜培养得到发酵液1.
野生型菌株scs-1用同样的方法发酵，培养基和阳极液中不加抗生素和诱导剂，过夜培养后得到发酵液2。
阳极液配置：
1)5XM9溶液：30g/L无水磷酸氢二钠、15g/L无水磷酸二氢钾、5g/L无水氯化铵、2.5g/L氯化钠，准确称量上述物质溶于相应体积的蒸馏水中，定容。高压灭菌锅121℃、灭菌30min，冷却后常温备用。
2)1M硫酸镁溶液：准确称取12.05g无水硫酸镁，溶于蒸馏水中，定容至100mL；或准确称取24.65g七水合硫酸镁，溶于蒸馏水中，定容至100mL。高压灭菌锅121℃、灭菌30min，冷却后常温备用。
3)1M乳酸钠溶液：称量18.68g 60％乳酸钠溶液，溶于蒸馏水中，定容到100mL，灭菌备用。
4)0.1M氯化钙溶液：称量1.1g无水氯化钙，溶于蒸馏水中，定容至100mL。灭菌备用。
配置阳极液(1L)：准确量取200mL的5×M9、50mL的LB液体无抗培养基、20mL的1M乳酸钠溶液、1mL的1M硫酸镁溶液、1mL的0.1M氯化钙溶液，加无菌蒸馏水混匀定容。该过程在超净台中进行，防止染菌。
用阳极液分别对得到的上述两种发酵液调节OD600为1，得到菌悬液1(scs-R5)和菌悬液2(scs-1)，备用。
利用菌悬液1和菌悬液2分别组装微生物燃料电池，并连接上2000欧姆电阻构成闭合回路，将其放入30℃恒温培养箱中静置培养，连接上数据采集器，记录两种菌株的输出电压，得到的电压数据如图3(A)所示。
当上述微生物燃料电池电压达到峰值并保持恒定时，测量电池的电化学性能，用循环伏安法测试电池，扫速为l mV/S，以氯化银电极为参比电极，得到电池的伏安循环曲线，(如图3(B)所示)。用线性扫描伏安法测试电池，以扫速为0.1mV/S，得到功率密度曲线(如图3(C)所示)和极化曲线(如图3(D)所示)。从上述图可以看出，重组菌scs-R5的最大输出电压为590±10mV，其最大功率密度为1200±50mW/m2，与野生型海藻希瓦氏scs-1相比重组菌scs-R5的输出电压更大，功率密度更大，内阻更低，这表明重组菌scs-R5具有更好的电子传递能力。
实施例3
重组菌scs-R5结合阳极材料在产电中的应用
在微生物燃料电池中常见的阳极材料有碳布，导电棉，石磨电极等，本发明中选择用的电极为导电棉，导电棉具有很大的比表面积，非常有利于菌体的附着和接收电子，此外为了增强导电棉的导电性，在本实验中选用碳纳米管导电棉复合电极(CNT-CC电极)，CNT-CC电极制作过程，向碳纳米管水性分散液1(碳纳米管质量百分比为0.2％)中加入表面活性剂十二烷基苯磺酸钠(SDBS)，SDBS的质量百分比为1％，超声处理30min，得到含有表面活性剂的分散液2，将导电棉浸泡在上述分散液2中30min，取出，放入烘箱中烘干，得到CNT-CC电极，即可用于组装电池使用。
将重组菌scs-R5菌悬液1(终OD600＝1)和氧化石墨烯(GO)溶液(终浓度为0.1mg/ml)加入电池中，将电池放在磁力搅拌器上，在30℃培养箱中培养。
当上述电池电压达到峰值时，取出CNT-CC测量生物膜，得到阳极的激光共聚焦三维图，以普通碳布(对照)为电极的生物膜厚度为270μm左右，如图4(A)所示；CNT-CC加氧化石墨烯阳极的生物膜厚度为595μm左右，如图4(B)所示；这表明CNT-CC具有更大的表面积，表面更加粗糙，有利于多层生物膜的附着，同时氧化石墨烯在阳极被还原，包裹菌体形成复合生物膜，此外碳纳米管对导电棉的修饰增加了导电棉的导电能力，使电池的电化学性能得到了进一步的提升，输出电压达到640±20mV，如图5(A)所示，电流密度达到5000mA/m2，如图5(B)所示；功率密度达到了2200±50mW/m2，如图5(C)所示,极化曲线如图5(D)所示。
一方面导电棉使得菌体在阳极上的生物膜厚度增加，菌体与阳极的电子交换更加密切，另一方面，碳纳米管的掺杂在富集的菌群中可以起到支撑与导电的作用，菌体之间，菌体与阳极之间的电子交换速度增加。结果表明，复合电极使得重组菌scs-R5的产电能力得到了增强，同时，也是目前报道的关于海藻希瓦氏菌的最高产电能力，对电极的修饰能够大幅度的提高微生物燃料电池的电流输出，电极修饰也将是未来研究的一个重要方向。
序列表
<110> 天津大学
<120> 一种产核黄素的重组海藻希瓦氏菌及在产电中的应用
<160> 7
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1197
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1
atgttccacc caatcgaaga agctttagat gctttaaaaa aaggtgaagt tatcatcgtt 60
gttgatgatg aagatcgtga aaacgaaggt gatttcgttg ctttagctga acacgctact 120
ccagaagtta tcaacttcat ggctactcac ggtcgtggtt taatctgtac tccattatct 180
gaagaaatcg ctgatcgttt agatttacac ccaatggttg aacacaacac tgattctcac 240
cacactgctt tcactgtttc tatcgatcac cgtgaaacta aaactggtat ctctgctcaa 300
gaacgttctt tcactgttca agctttatta gattctaaat ctgttccatc tgatttccaa 360
cgtccaggtc acatcttccc attaatcgct aaaaaaggtg gtgttttaaa acgtgctggt 420
cacactgaag ctgctgttga tttagctgaa gcttgtggtt ctccaggtgc tggtgttatc 480
tgtgaaatca tgaacgaaga tggtactatg gctcgtgttc cagaattaat cgaaatcgct 540
aaaaaacacc aattaaaaat gatcactatc aaagatttaa tccaataccg ttacaactta 600
actactttag ttgaacgtga agttgatatc actttaccaa ctgatttcgg tactttcaaa 660
gtttacggtt acactaacga agttgatggt aaagaacacg ttgctttcgt tatgggtgat 720
gttccattcg gtgaagaacc agttttagtt cgtgttcact ctgaatgttt aactggtgat 780
gttttcggtt ctcaccgttg tgattgtggt ccacaattac acgctgcttt aaaccaaatc 840
gctgctgaag gtcgtggtgt tttattatac ttacgtcaag aaggtcgtgg tatcggttta 900
atcaacaaat taaaagctta caaattacaa gaacaaggtt acgatactgt tgaagctaac 960
gaagctttag gtttcttacc agatttacgt aactacggta tcggtgctca aatcttacgt 1020
gatttaggtg ttcgtaacat gaaattatta actaacaacc cacgtaaaat cgctggttta 1080
gaaggttacg gtttatctat ctctgaacgt gttccattac aaatggaagc taaagaacac 1140
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gtgaaaacta tccacatcac tcacccacac cacttaatca aagaagaaca agctaaatct 60
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gctaaacaaa tcgctgaaga aaaaggttta actttagctg ttatgacttt ccacccacac 180
ccatctcacg ttttaggtcg tgataaagaa ccaaaagatt taatcactcc attagaagat 240
aaaatcaacc aaatcgaaca attaggtact gaagttttat acgttgttaa attcaacgaa 300
gttttcgctt ctttatctcc aaaacaattc atcgatcaat acatcatcgg tttaaacgtt 360
caacacgctg ttgctggttt cgatttcact tacggtaaat acggtaaagg tactatgaaa 420
actatgccag atgatttaga tggtaaagct ggttgtacta tggttgaaaa attaactgaa 480
caagataaaa aaatctcttc ttcttacatc cgtactgctt tacaaaacgg tgatgttgaa 540
ttagctaacg ttttattagg tcaaccatac ttcatcaaag gtatcgttat ccacggtgat 600
aaacgtggtc gtactatcgg tttcccaact gctaacgttg gtttaaacaa ctcttacatc 660
gttccaccaa ctggtgttta cgctgttaaa gctgaagtta acggtgaagt ttacaacggt 720
gtttgtaaca tcggttacaa accaactttc tacgaaaaac gtccagaaca accatctatc 780
gaagttaact tattcgattt caaccaagaa gtttacggtg ctgctatcaa aatcgaatgg 840
tacaaacgta tccgttctga acgtaaattc aacggtatca aagaattaac tgaacaaatc 900
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atggaagaat actacatgaa attagcttta gatttagcta aacaaggtga aggtcaaact 60
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gctcacttaa aatacggtga agctcacgct gaagttcacg ctatccacat ggctggtgct 180
cacgctgaag gtgctgatat ctacgttact ttagaaccat gttctcacta cggtaaaact 240
ccaccatgtg ctgaattaat catcaactct ggtatcaaac gtgttttcgt tgctatgcgt 300
gatccaaacc cattagttgc tggtcgtggt atctctatga tgaaagaagc tggtatcgaa 360
gttcgtgaag gtatcttagc tgatcaagct gaacgtttaa acgaaaaatt cttacacttc 420
atgcgtactg gtttaccata cgttacttta aaagctgctg cttctttaga tggtaaaatc 480
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caataccgta aaactcacca atctatctta gttggtgttg gtactgttaa agctgataac 600
ccatctttaa cttgtcgttt accaaacgtt actaaacaac cagttcgtgt tatcttagat 660
actgttttat ctatcccaga agatgctaaa gttatctgtg atcaaatcgc tccaacttgg 720
atcttcacta ctgctcgtgc tgatgaagaa aaaaaaaaac gtttatctgc tttcggtgtt 780
aacatcttca ctttagaaac tgaacgtatc caaatcccag atgttttaaa aatcttagct 840
gaagaaggta tcatgtctgt ttacgttgaa ggtggttctg ctgttcacgg ttctttcgtt 900
aaagaaggtt gtttccaaga aatcatcttc tacttcgctc caaaattaat cggtggtact 960
cacgctccat ctttaatctc tggtgaaggt ttccaatcta tgaaagatgt tccattatta 1020
caattcactg atatcactca aatcggtcgt gatatcaaat taactgctaa accaactaaa 1080
gaataa 1086
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atgttcactg gtatcatcga agaaactggt actatcgaat ctatgaaaaa agctggtcac 60
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atcgctgtta acggtatctg tttaactgtt actgatttca ctaaaaacca attcactgtt 180
gatgttatgc cagaaactgt taaagctact tctttaaacg atttaactaa aggttctaaa 240
gttaacttag aacgtgctat ggctgctaac ggtcgtttcg gtggtcactt cgtttctggt 300
cacgttgatg gtactgctga aatcactcgt atcgaagaaa aatctaacgc tgtttactac 360
gatttaaaaa tggatccatc tttaactaaa actttagttt taaaaggttc tatcactgtt 420
gatggtgttt ctttaactat cttcggttta actgaagata ctgttactat ctctttaatc 480
ccacacacta tctctgaaac tatcttctct gaaaaaacta tcggttctaa agttaacatc 540
gaatgtgata tgatcggtaa atacatgtac cgtttcttac acaaagctaa cgaaaacaaa 600
actcaacaaa ctatcactaa agctttctta tctgaaaacg gtttctaac 649
<210> 5
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atgaacatca tccaaggtaa cttagttggt actggtttaa aaatcggtat cgttgttggt 60
cgtttcaacg atttcatcac ttctaaatta ttatctggtg ctgaagatgc tttattacgt 120
cacggtgttg atactaacga tatcgatgtt gcttgggttc caggtgcttt cgaaatccca 180
ttcgctgcta aaaaaatggc tgaaactaaa aaatacgatg ctatcatcac tttaggtact 240
gttatccgtg gtgctactac tcactacgat tacgtttgta acgaagctgc taaaggtatc 300
gctcaagctg ctaacactac tggtgttcca gttatcttcg gtatcgttac tactgaaaac 360
atcgaacaag ctatcgaacg tgctggtact aaagctggta acaaaggtgt tgattgtgct 420
gtttctgcta tcgaaatggc taacttaaac cgttctttcg aataa 465
<210> 6
<211> 5904
<212> DNA
<213> 人工序列(Artificial Sequence)
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cacctcgcta acggattcac cgtttttatc aggctctggg aggcagaata aatgatcata 60
tcgtcaatta ttacctccac ggggagagcc tgagcaaact ggcctcaggc atttgagaag 120
cacacggtca cactgcttcc ggtagtcaat aaaccggtca gaatttcaga taaaaaaaat 180
ccttagcttt cgctaaggat gatttctgtg gtacctcgga tcccggggag ctagcacgaa 240
ttcgcggccg cttctagacc gacaccatcg aatggtgcaa aacctttcgc ggtatggcat 300
gatagcgccc ggaagagagt caattcaggg tggtgaatgt gaaaccagta acgttatacg 360
atgtcgcaga gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca 420
gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag ctgaattaca 480
ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc gttgctgatt ggcgttgcca 540
cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg 600
atcaactggg tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta 660
aagcggcggt gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc 720
tggatgacca ggatgccatt gctgtggaag ctgcctgcac taatgttccg gcgttatttc 780
ttgatgtctc tgaccagaca cccatcaaca gtattatttt ctcccatgaa gacggtacgc 840
gactgggcgt ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc 900
cattaagttc tgtctcggcg cgtctgcgtc tggctggctg gcataaatat ctcactcgca 960
atcaaattca gccgatagcg gaacgggaag gcgactggag tgccatgtcc ggttttcaac 1020
aaaccatgca aatgctgaat gagggcatcg ttcccactgc gatgctggtt gccaacgatc 1080
agatggcgct gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata 1140
tctcggtagt gggatacgac gataccgaag acagctcatg ttatatcccg ccgttaacca 1200
ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct 1260
ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa 1320
ccaccctggc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc 1380
agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgta 1440
agttagctca ctcattaggc acaattctca tgtttgacag cttatcatcg actgcacggt 1500
gcaccaatgc ttctggcgtc aggcagccat cggaagctgt ggtatggctg tgcaggtcgt 1560
aaatcactgc ataattcgtg tcgctcaagg cgcactcccg ttctggataa tgttttttgc 1620
gccgacatca taacggttct ggcaaatatt ctgaaatgag ctgttgacaa ttaatcatcg 1680
gctcgtataa tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagccagtcc 1740
gtttaggtgt tttcacgagc acttcaccaa caaggaccat agcatatgcc actagtagcg 1800
gccgcctgca ggtggtcgac cactcgaggc caggcatcaa ataaaacgaa aggctcagtc 1860
gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 1920
cactggctca ccttcgggtg ggcctttctg cgtttataac cggtaaacca gcaatagaca 1980
taagcggcta tttaacgacc ctgccctgaa ccgacgaccg ggtcgaattt gctttcgaac 2040
cccagagtcc cgctcagaag aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat 2100
cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg ccaagctctt 2160
cagcaatatc acgggtagcc aacgctatgt cctgatagcg gtccgccaca cccagccggc 2220
cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc aagcaggcat 2280
cgccatgggt cacgacgaga tcctcgccgt cgggcatgcg cgccttgagc ctggcgaaca 2340
gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg acaagaccgg 2400
cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg 2460
tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat actttctcgg 2520
caggagcaag gtgagatgac aggagatcct gccccggcac ttcgcccaat agcagccagt 2580
cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaacgccc gtcgtggcca 2640
gccacgatag ccgcgctgcc tcgtcctgca gttcattcag ggcaccggac aggtcggtct 2700
tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa cacggcggca tcagagcagc 2760
cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcg gccggagaac 2820
ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc tcatcctgtc tcttgatcag 2880
atcttgatcc cctgcgccat cagatccttg gcggcaagaa agccatccag tttactttgc 2940
agggcttccc aaccttacca gagggcgccc cagctggcaa ttccggttcg cttgctgtcc 3000
ataaaaccgc ccagtctagc tatcgccatg taagcccact gcaagctacc tgctttctct 3060
ttgcgcttgc gttttccctt gtccagatag cccagtagct gacattcatc ccaggtggca 3120
cttttcgggg aaatgtgcgc gcccgcgttc ctgctggcgc tgggcctgtt tctggcgctg 3180
gacttcccgc tgttccgtca gcagcttttc gcccacggcc ttgatgatcg cggcggcctt 3240
ggcctgcata tcccgattca acggccccag ggcgtccaga acgggcttca ggcgctcccg 3300
aaggtctcgg gccgtctctt gggcttgatc ggccttcttg cgcatctcac gcgctcctgc 3360
ggcggcctgt agggcaggct catacccctg ccgaaccgct tttgtcagcc ggtcggccac 3420
ggcttccggc gtctcaacgc gctttgagat tcccagcttt tcggccaatc cctgcggtgc 3480
ataggcgcgt ggctcgaccg cttgcgggct gatggtgacg tggcccactg gtggccgctc 3540
cagggcctcg tagaacgcct gaatgcgcgt gtgacgtgcc ttgctgccct cgatgccccg 3600
ttgcagccct agatcggcca cagcggccgc aaacgtggtc tggtcgcggg tcatctgcgc 3660
tttgttgccg atgaactcct tggccgacag cctgccgtcc tgcgtcagcg gcaccacgaa 3720
cgcggtcatg tgcgggctgg tttcgtcacg gtggatgctg gccgtcacga tgcgatccgc 3780
cccgtacttg tccgccagcc acttgtgcgc cttctcgaag aacgccgcct gctgttcttg 3840
gctggccgac ttccaccatt ccgggctggc cgtcatgacg tactcgaccg ccaacacagc 3900
gtccttgcgc cgcttctctg gcagcaactc gcgcagtcgg cccatcgctt catcggtgct 3960
gctggccgcc cagtgctcgt tctctggcgt cctgctggcg tcagcgttgg gcgtctcgcg 4020
ctcgcggtag gcgtgcttga gactggccgc cacgttgccc attttcgcca gcttcttgca 4080
tcgcatgatc gcgtatgccg ccatgcctgc ccctcccttt tggtgtccaa ccggctcgac 4140
gggggcagcg caaggcggtg cctccggcgg gccactcaat gcttgagtat actcactaga 4200
ctttgcttcg caaagtcgtg accgcctacg gcggctgcgg cgccctacgg gcttgctctc 4260
cgggcttcgc cctgcgcggt cgctgcgctc ccttgccagc ccgtggatat gtggacgatg 4320
gccgcgagcg gccaccggct ggctcgcttc gctcggcccg tggacaaccc tgctggacaa 4380
gctgatggac aggctgcgcc tgcccacgag cttgaccaca gggattgccc accggctacc 4440
cagccttcga ccacataccc accggctcca actgcgcggc ctgcggcctt gccccatcaa 4500
tttttttaat tttctctggg gaaaagcctc cggcctgcgg cctgcgcgct tcgcttgccg 4560
gttggacacc aagtggaagg cgggtcaagg ctcgcgcagc gaccgcgcag cggcttggcc 4620
ttgacgcgcc tggaacgacc caagcctatg cgagtggggg cagtcgaagg cgaagcccgc 4680
ccgcctgccc cccgagcctc acggcggcga gtgcgggggt tccaaggggg cagcgccacc 4740
ttgggcaagg ccgaaggccg cgcagtcgat caacaagccc cggaggggcc actttttgcc 4800
ggagggggag ccgcgccgaa ggcgtggggg aaccccgcag gggtgccctt ctttgggcac 4860
caaagaacta gatatagggc gaaatgcgaa agacttaaaa atcaacaact taaaaaaggg 4920
gggtacgcaa cagctcattg cggcaccccc cgcaatagct cattgcgtag gttaaagaaa 4980
atctgtaatt gactgccact tttacgcaac gcataattgt tgtcgcgctg ccgaaaagtt 5040
gcagctgatt gcgcatggtg ccgcaaccgt gcggcaccct accgcatgga gataagcatg 5100
gccacgcagt ccagagaaat cggcattcaa gccaagaaca agcccggtca ctgggtgcaa 5160
acggaacgca aagcgcatga ggcgtgggcc gggcttattg cgaggaaacc cacggcggca 5220
atgctgctgc atcacctcgt ggcgcagatg ggccaccaga acgccgtggt ggtcagccag 5280
aagacacttt ccaagctcat cggacgttct ttgcggacgg tccaatacgc agtcaaggac 5340
ttggtggccg agcgctggat ctccgtcgtg aagctcaacg gccccggcac cgtgtcggcc 5400
tacgtggtca atgaccgcgt ggcgtggggc cagccccgcg accagttgcg cctgtcggtg 5460
ttcagtgccg ccgtggtggt tgatcacgac gaccaggacg aatcgctgtt ggggcatggc 5520
gacctgcgcc gcatcccgac cctgtatccg ggcgagcagc aactaccgac cggccccggc 5580
gaggagccgc ccagccagcc cggcattccg ggcatggaac cagacctgcc agccttgacc 5640
gaaacggagg aatgggaacg gcgcgggcag cagcgcctgc cgatgcccga tgagccgtgt 5700
tttctggacg atggcgagcc gttggagccg ccgacacggg tcacgctgcc gcgccggtag 5760
cacttgggtt gcgcagcaac ccgtaagtgc gctgttccag actatcggct gtagccgcct 5820
cgccgcccta taccttgtct gcctccccgc gttgcgtcgc ggtgcatgga gccgggccac 5880
ctcgacctga atggaagccg gcgg 5904
<210> 7
<211> 10450
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 7
acctcgctaa cggattcacc gtttttatca ggctctggga ggcagaataa atgatcatat 60
cgtcaattat tacctccacg gggagagcct gagcaaactg gcctcaggca tttgagaagc 120
acacggtcac actgcttccg gtagtcaata aaccggtcag aatttcagat aaaaaaaatc 180
cttagctttc gctaaggatg atttctgtgg tacctcggat cccggggagc tagcacgaat 240
tcgcggccgc ttctagaccg acaccatcga atggtgcaaa acctttcgcg gtatggcatg 300
atagcgcccg gaagagagtc aattcagggt ggtgaatgtg aaaccagtaa cgttatacga 360
tgtcgcagag tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag 420
ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat 480
tcccaaccgc gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac 540
ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga 600
tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa 660
agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct 720
ggatgaccag gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct 780
tgatgtctct gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg 840
actgggcgtg gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc 900
attaagttct gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa 960
tcaaattcag ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca 1020
aaccatgcaa atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca 1080
gatggcgctg ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggatat 1140
ctcggtagtg ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac 1200
catcaaacag gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc 1260
tcagggccag gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac 1320
caccctggcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca 1380
gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtaa 1440
gttagctcac tcattaggca caattctcat gtttgacagc ttatcatcga ctgcacggtg 1500
caccaatgct tctggcgtca ggcagccatc ggaagctgtg gtatggctgt gcaggtcgta 1560
aatcactgca taattcgtgt cgctcaaggc gcactcccgt tctggataat gttttttgcg 1620
ccgacatcat aacggttctg gcaaatattc tgaaatgagc tgttgacaat taatcatcgg 1680
ctcgtataat gtgtggaatt gtgagcggat aacaatttca cacaggaaac agccagtccg 1740
tttaggtgtt ttcacgagca cttcaccaac aaggaccata gcatatggga ctagagaaag 1800
aggagaaatc tagtatgttc cacccaatcg aagaagcttt agatgcttta aaaaaaggtg 1860
aagttatcat cgttgttgat gatgaagatc gtgaaaacga aggtgatttc gttgctttag 1920
ctgaacacgc tactccagaa gttatcaact tcatggctac tcacggtcgt ggtttaatct 1980
gtactccatt atctgaagaa atcgctgatc gtttagattt acacccaatg gttgaacaca 2040
acactgattc tcaccacact gctttcactg tttctatcga tcaccgtgaa actaaaactg 2100
gtatctctgc tcaagaacgt tctttcactg ttcaagcttt attagattct aaatctgttc 2160
catctgattt ccaacgtcca ggtcacatct tcccattaat cgctaaaaaa ggtggtgttt 2220
taaaacgtgc tggtcacact gaagctgctg ttgatttagc tgaagcttgt ggttctccag 2280
gtgctggtgt tatctgtgaa atcatgaacg aagatggtac tatggctcgt gttccagaat 2340
taatcgaaat cgctaaaaaa caccaattaa aaatgatcac tatcaaagat ttaatccaat 2400
accgttacaa cttaactact ttagttgaac gtgaagttga tatcacttta ccaactgatt 2460
tcggtacttt caaagtttac ggttacacta acgaagttga tggtaaagaa cacgttgctt 2520
tcgttatggg tgatgttcca ttcggtgaag aaccagtttt agttcgtgtt cactctgaat 2580
gtttaactgg tgatgttttc ggttctcacc gttgtgattg tggtccacaa ttacacgctg 2640
ctttaaacca aatcgctgct gaaggtcgtg gtgttttatt atacttacgt caagaaggtc 2700
gtggtatcgg tttaatcaac aaattaaaag cttacaaatt acaagaacaa ggttacgata 2760
ctgttgaagc taacgaagct ttaggtttct taccagattt acgtaactac ggtatcggtg 2820
ctcaaatctt acgtgattta ggtgttcgta acatgaaatt attaactaac aacccacgta 2880
aaatcgctgg tttagaaggt tacggtttat ctatctctga acgtgttcca ttacaaatgg 2940
aagctaaaga acacaacaaa aaatacttac aaactaaaat gaacaaatta ggtcacttat 3000
tacacttcta atactagaga aagaggagaa aactagaatg gaagaatact acatgaaatt 3060
agctttagat ttagctaaac aaggtgaagg tcaaactgaa tctaacccat tagttggtgc 3120
tgttgttgtt aaagatggtc aaatcgttgg tatgggtgct cacttaaaat acggtgaagc 3180
tcacgctgaa gttcacgcta tccacatggc tggtgctcac gctgaaggtg ctgatatcta 3240
cgttacttta gaaccatgtt ctcactacgg taaaactcca ccatgtgctg aattaatcat 3300
caactctggt atcaaacgtg ttttcgttgc tatgcgtgat ccaaacccat tagttgctgg 3360
tcgtggtatc tctatgatga aagaagctgg tatcgaagtt cgtgaaggta tcttagctga 3420
tcaagctgaa cgtttaaacg aaaaattctt acacttcatg cgtactggtt taccatacgt 3480
tactttaaaa gctgctgctt ctttagatgg taaaatcgct acttctactg gtgattctaa 3540
atggatcact tctgaagctg ctcgtcaaga tgctcaacaa taccgtaaaa ctcaccaatc 3600
tatcttagtt ggtgttggta ctgttaaagc tgataaccca tctttaactt gtcgtttacc 3660
aaacgttact aaacaaccag ttcgtgttat cttagatact gttttatcta tcccagaaga 3720
tgctaaagtt atctgtgatc aaatcgctcc aacttggatc ttcactactg ctcgtgctga 3780
tgaagaaaaa aaaaaacgtt tatctgcttt cggtgttaac atcttcactt tagaaactga 3840
acgtatccaa atcccagatg ttttaaaaat cttagctgaa gaaggtatca tgtctgttta 3900
cgttgaaggt ggttctgctg ttcacggttc tttcgttaaa gaaggttgtt tccaagaaat 3960
catcttctac ttcgctccaa aattaatcgg tggtactcac gctccatctt taatctctgg 4020
tgaaggtttc caatctatga aagatgttcc attattacaa ttcactgata tcactcaaat 4080
cggtcgtgat atcaaattaa ctgctaaacc aactaaagaa taatactaga ggagctgttg 4140
acaattaatc atcggctcgt ataatgtgtg gaattgtgag cggataacaa tttactagag 4200
aaagaggaga aactgcatat gttcactggt atcatcgaag aaactggtac tatcgaatct 4260
atgaaaaaag ctggtcacgc tatggcttta actatcaaat gttctaaaat cttagaagat 4320
gttcacttag gtgattctat cgctgttaac ggtatctgtt taactgttac tgatttcact 4380
aaaaaccaat tcactgttga tgttatgcca gaaactgtta aagctacttc tttaaacgat 4440
ttaactaaag gttctaaagt taacttagaa cgtgctatgg ctgctaacgg tcgtttcggt 4500
ggtcacttcg tttctggtca cgttgatggt actgctgaaa tcactcgtat cgaagaaaaa 4560
tctaacgctg tttactacga tttaaaaatg gatccatctt taactaaaac tttagtttta 4620
aaaggttcta tcactgttga tggtgtttct ttaactatct tcggtttaac tgaagatact 4680
gttactatct ctttaatccc acacactatc tctgaaacta tcttctctga aaaaactatc 4740
ggttctaaag ttaacatcga atgtgatatg atcggtaaat acatgtaccg tttcttacac 4800
aaagctaacg aaaacaaaac tcaacaaact atcactaaag ctttcttatc tgaaaacggt 4860
ttctaatact agagaaagag gagaaatcta gtatgaacat catccaaggt aacttagttg 4920
gtactggttt aaaaatcggt atcgttgttg gtcgtttcaa cgatttcatc acttctaaat 4980
tattatctgg tgctgaagat gctttattac gtcacggtgt tgatactaac gatatcgatg 5040
ttgcttgggt tccaggtgct ttcgaaatcc cattcgctgc taaaaaaatg gctgaaacta 5100
aaaaatacga tgctatcatc actttaggta ctgttatccg tggtgctact actcactacg 5160
attacgtttg taacgaagct gctaaaggta tcgctcaagc tgctaacact actggtgttc 5220
cagttatctt cggtatcgtt actactgaaa acatcgaaca agctatcgaa cgtgctggta 5280
ctaaagctgg taacaaaggt gttgattgtg ctgtttctgc tatcgaaatg gctaacttaa 5340
accgttcttt cgaataatac tagagaaaga ggagaaaact agagtgaaaa ctatccacat 5400
cactcaccca caccacttaa tcaaagaaga acaagctaaa tctgttatgg ctttaggtta 5460
cttcgatggt gttcacttag gtcaccaaaa agttatcggt actgctaaac aaatcgctga 5520
agaaaaaggt ttaactttag ctgttatgac tttccaccca cacccatctc acgttttagg 5580
tcgtgataaa gaaccaaaag atttaatcac tccattagaa gataaaatca accaaatcga 5640
acaattaggt actgaagttt tatacgttgt taaattcaac gaagttttcg cttctttatc 5700
tccaaaacaa ttcatcgatc aatacatcat cggtttaaac gttcaacacg ctgttgctgg 5760
tttcgatttc acttacggta aatacggtaa aggtactatg aaaactatgc cagatgattt 5820
agatggtaaa gctggttgta ctatggttga aaaattaact gaacaagata aaaaaatctc 5880
ttcttcttac atccgtactg ctttacaaaa cggtgatgtt gaattagcta acgttttatt 5940
aggtcaacca tacttcatca aaggtatcgt tatccacggt gataaacgtg gtcgtactat 6000
cggtttccca actgctaacg ttggtttaaa caactcttac atcgttccac caactggtgt 6060
ttacgctgtt aaagctgaag ttaacggtga agtttacaac ggtgtttgta acatcggtta 6120
caaaccaact ttctacgaaa aacgtccaga acaaccatct atcgaagtta acttattcga 6180
tttcaaccaa gaagtttacg gtgctgctat caaaatcgaa tggtacaaac gtatccgttc 6240
tgaacgtaaa ttcaacggta tcaaagaatt aactgaacaa atcgaaaaag ataaacaaga 6300
agctatccgt tacttctcta acttacgtaa ataatactag tagcggccgc ctgcaggtgg 6360
tcgaccactc gaggccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt 6420
tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc 6480
gggtgggcct ttctgcgttt ataaccggta aaccagcaat agacataagc ggctatttaa 6540
cgaccctgcc ctgaaccgac gaccgggtcg aatttgcttt cgaaccccag agtcccgctc 6600
agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga gcggcgatac 6660
cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca atatcacggg 6720
tagccaacgc tatgtcctga tagcggtccg ccacacccag ccggccacag tcgatgaatc 6780
cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgcca tgggtcacga 6840
cgagatcctc gccgtcgggc atgcgcgcct tgagcctggc gaacagttcg gctggcgcga 6900
gcccctgatg ctcttcgtcc agatcatcct gatcgacaag accggcttcc atccgagtac 6960
gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg 7020
tatgcagccg ccgcattgca tcagccatga tggatacttt ctcggcagga gcaaggtgag 7080
atgacaggag atcctgcccc ggcacttcgc ccaatagcag ccagtccctt cccgcttcag 7140
tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg 7200
ctgcctcgtc ctgcagttca ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg 7260
ggcgcccctg cgctgacagc cggaacacgg cggcatcaga gcagccgatt gtctgttgtg 7320
cccagtcata gccgaatagc ctctccaccc aagcggccgg agaacctgcg tgcaatccat 7380
cttgttcaat catgcgaaac gatcctcatc ctgtctcttg atcagatctt gatcccctgc 7440
gccatcagat ccttggcggc aagaaagcca tccagtttac tttgcagggc ttcccaacct 7500
taccagaggg cgccccagct ggcaattccg gttcgcttgc tgtccataaa accgcccagt 7560
ctagctatcg ccatgtaagc ccactgcaag ctacctgctt tctctttgcg cttgcgtttt 7620
cccttgtcca gatagcccag tagctgacat tcatcccagg tggcactttt cggggaaatg 7680
tgcgcgcccg cgttcctgct ggcgctgggc ctgtttctgg cgctggactt cccgctgttc 7740
cgtcagcagc ttttcgccca cggccttgat gatcgcggcg gccttggcct gcatatcccg 7800
attcaacggc cccagggcgt ccagaacggg cttcaggcgc tcccgaaggt ctcgggccgt 7860
ctcttgggct tgatcggcct tcttgcgcat ctcacgcgct cctgcggcgg cctgtagggc 7920
aggctcatac ccctgccgaa ccgcttttgt cagccggtcg gccacggctt ccggcgtctc 7980
aacgcgcttt gagattccca gcttttcggc caatccctgc ggtgcatagg cgcgtggctc 8040
gaccgcttgc gggctgatgg tgacgtggcc cactggtggc cgctccaggg cctcgtagaa 8100
cgcctgaatg cgcgtgtgac gtgccttgct gccctcgatg ccccgttgca gccctagatc 8160
ggccacagcg gccgcaaacg tggtctggtc gcgggtcatc tgcgctttgt tgccgatgaa 8220
ctccttggcc gacagcctgc cgtcctgcgt cagcggcacc acgaacgcgg tcatgtgcgg 8280
gctggtttcg tcacggtgga tgctggccgt cacgatgcga tccgccccgt acttgtccgc 8340
cagccacttg tgcgccttct cgaagaacgc cgcctgctgt tcttggctgg ccgacttcca 8400
ccattccggg ctggccgtca tgacgtactc gaccgccaac acagcgtcct tgcgccgctt 8460
ctctggcagc aactcgcgca gtcggcccat cgcttcatcg gtgctgctgg ccgcccagtg 8520
ctcgttctct ggcgtcctgc tggcgtcagc gttgggcgtc tcgcgctcgc ggtaggcgtg 8580
cttgagactg gccgccacgt tgcccatttt cgccagcttc ttgcatcgca tgatcgcgta 8640
tgccgccatg cctgcccctc ccttttggtg tccaaccggc tcgacggggg cagcgcaagg 8700
cggtgcctcc ggcgggccac tcaatgcttg agtatactca ctagactttg cttcgcaaag 8760
tcgtgaccgc ctacggcggc tgcggcgccc tacgggcttg ctctccgggc ttcgccctgc 8820
gcggtcgctg cgctcccttg ccagcccgtg gatatgtgga cgatggccgc gagcggccac 8880
cggctggctc gcttcgctcg gcccgtggac aaccctgctg gacaagctga tggacaggct 8940
gcgcctgccc acgagcttga ccacagggat tgcccaccgg ctacccagcc ttcgaccaca 9000
tacccaccgg ctccaactgc gcggcctgcg gccttgcccc atcaattttt ttaattttct 9060
ctggggaaaa gcctccggcc tgcggcctgc gcgcttcgct tgccggttgg acaccaagtg 9120
gaaggcgggt caaggctcgc gcagcgaccg cgcagcggct tggccttgac gcgcctggaa 9180
cgacccaagc ctatgcgagt gggggcagtc gaaggcgaag cccgcccgcc tgccccccga 9240
gcctcacggc ggcgagtgcg ggggttccaa gggggcagcg ccaccttggg caaggccgaa 9300
ggccgcgcag tcgatcaaca agccccggag gggccacttt ttgccggagg gggagccgcg 9360
ccgaaggcgt gggggaaccc cgcaggggtg cccttctttg ggcaccaaag aactagatat 9420
agggcgaaat gcgaaagact taaaaatcaa caacttaaaa aaggggggta cgcaacagct 9480
cattgcggca ccccccgcaa tagctcattg cgtaggttaa agaaaatctg taattgactg 9540
ccacttttac gcaacgcata attgttgtcg cgctgccgaa aagttgcagc tgattgcgca 9600
tggtgccgca accgtgcggc accctaccgc atggagataa gcatggccac gcagtccaga 9660
gaaatcggca ttcaagccaa gaacaagccc ggtcactggg tgcaaacgga acgcaaagcg 9720
catgaggcgt gggccgggct tattgcgagg aaacccacgg cggcaatgct gctgcatcac 9780
ctcgtggcgc agatgggcca ccagaacgcc gtggtggtca gccagaagac actttccaag 9840
ctcatcggac gttctttgcg gacggtccaa tacgcagtca aggacttggt ggccgagcgc 9900
tggatctccg tcgtgaagct caacggcccc ggcaccgtgt cggcctacgt ggtcaatgac 9960
cgcgtggcgt ggggccagcc ccgcgaccag ttgcgcctgt cggtgttcag tgccgccgtg 10020
gtggttgatc acgacgacca ggacgaatcg ctgttggggc atggcgacct gcgccgcatc 10080
ccgaccctgt atccgggcga gcagcaacta ccgaccggcc ccggcgagga gccgcccagc 10140
cagcccggca ttccgggcat ggaaccagac ctgccagcct tgaccgaaac ggaggaatgg 10200
gaacggcgcg ggcagcagcg cctgccgatg cccgatgagc cgtgttttct ggacgatggc 10260
gagccgttgg agccgccgac acgggtcacg ctgccgcgcc ggtagcactt gggttgcgca 10320
gcaacccgta agtgcgctgt tccagactat cggctgtagc cgcctcgccg ccctatacct 10380
tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga cctgaatgga 10440
agccggcggc 10450

Claims (2)

1.一种产核黄素的重组海藻希瓦氏菌（Shewanella algae），其特征是用下述方法构建：
(1)将黄素合成途径中的五个目的基因ribA，ribC，ribD，ribE，ribH连接到载体pyydt上，得到重组质粒R5；
所述基因ribA的核苷酸序列用SEQ ID NO.1所示；
所述基因ribC的核苷酸序列用SEQ ID NO.2所示；
所述基因ribD的核苷酸序列用SEQ ID NO.3所示；
所述基因ribE的核苷酸序列用SEQ ID NO.4所示；
所述基因ribH的核苷酸序列用SEQ ID NO.5所示；
所述载体pyydt的核苷酸序列用SEQ ID NO.6所示；
所述重组质粒R5的核苷酸序列用SEQ ID NO.7所示；
(2)将重组质粒R5，转入海藻希瓦氏菌（Shewanella algae）scs-1菌株，该菌株保藏编号：CGMCC No. 18696，得到一种产核黄素的重组海藻希瓦氏菌scs-R5。
2.权利要求1所述的一种产核黄素的重组海藻希瓦氏菌在产电中的应用。
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CN114591999B (zh) * 2020-12-03 2023-07-21 上海市农业科学院 RibAS、RibBS、RibGS和RibHS多基因组合在水稻中提高VB2含量应用及方法
CN114540396A (zh) * 2022-02-24 2022-05-27 天津大学 希瓦氏菌株中葡萄糖代谢通路的构建方法

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014197708A1 (en) * 2013-06-06 2014-12-11 River Road Research, Inc. Methods for producing melanin and inorganic fertilizer from fermentation leachates
CN104762230A (zh) * 2015-03-26 2015-07-08 华中农业大学 防治作物储藏期黄曲霉菌及毒素的海藻希瓦氏菌及其应用
JP2015191856A (ja) * 2014-03-28 2015-11-02 イビデン株式会社 微生物燃料電池用アノード電極の製造方法
WO2016125098A1 (en) * 2015-02-05 2016-08-11 Arturo Solis Herrera Process and cell for generating electric current
CN110511882A (zh) * 2019-07-16 2019-11-29 天津科技大学 一种耐盐且具有产电特性的菌株及其在微生物燃料电池中的应用
CN111548969A (zh) * 2020-05-29 2020-08-18 天津大学 一株海藻希瓦氏菌scs-1及其在微生物产电中的应用

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014197708A1 (en) * 2013-06-06 2014-12-11 River Road Research, Inc. Methods for producing melanin and inorganic fertilizer from fermentation leachates
JP2015191856A (ja) * 2014-03-28 2015-11-02 イビデン株式会社 微生物燃料電池用アノード電極の製造方法
WO2016125098A1 (en) * 2015-02-05 2016-08-11 Arturo Solis Herrera Process and cell for generating electric current
CN104762230A (zh) * 2015-03-26 2015-07-08 华中农业大学 防治作物储藏期黄曲霉菌及毒素的海藻希瓦氏菌及其应用
CN110511882A (zh) * 2019-07-16 2019-11-29 天津科技大学 一种耐盐且具有产电特性的菌株及其在微生物燃料电池中的应用
CN111548969A (zh) * 2020-05-29 2020-08-18 天津大学 一株海藻希瓦氏菌scs-1及其在微生物产电中的应用

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
An efficient microbial fuel cell using a CNT–RTIL based nanocomposite;Leila Mahrokh等;《Journal of Materials Chemistry A》;20170531;第5卷;全文 *
Shewanella algae Relatives Capable of Generating Electricity from Acetate Contribute to Coastal-Sediment Microbial Fuel Cells Treating Complex Organic Matter;Inohana Yoshino等;《Microbes and environments》;20200331;第35卷;全文 *
一株单环刺螠肠道电活性希瓦氏菌Shewanella marisflavi的生理学特性;李新等;《微生物学报》;20200121(第07期);全文 *
一株耐盐产电菌Shewanella algae E-1的分离及其产电特性分析;黄亦馨等;《微生物学通报》;20191017(第02期);全文 *
海水养殖环境产电菌的筛选及其产电性能测定;王再明;《中国学位论文全文数据库》;20201109;全文 *
海藻希瓦氏菌对Zn-Al-Cd牺牲阳极的腐蚀性能影响;张杰等;《金属学报》;20121211(第12期);全文 *
铁还原细菌Shewanella algae生物膜对316L不锈钢腐蚀行为的影响;杜向前等;《中国腐蚀与防护学报》;20131015(第05期);全文 *

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