CN111534431A

CN111534431A - Cell culture recovery equipment

Info

Publication number: CN111534431A
Application number: CN202010407471.XA
Authority: CN
Inventors: 陈龙刚
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-05-14
Filing date: 2020-05-14
Publication date: 2020-08-14

Abstract

The invention relates to the technical field of cell recovery, in particular to cell culture recovery equipment, which solves the problems that in the prior art, time is wasted due to single operation, manual operation is performed, and if liquid nitrogen is filled into a test tube, the test tube is easy to break, and further, human injury accidents are possibly caused. The utility model provides a cell culture recovery equipment, includes the casing, and the top of casing is equipped with the upper cover, and the inside of casing is equipped with places the dish, places to be equipped with elevating system between dish and the casing, and the surface of placing the dish is equipped with a plurality of and is centrosymmetric distribution's test-tube clamp, and the bottom of casing is equipped with the landing leg, is equipped with actuating mechanism between landing leg and the casing. The test tube clamp clamps a plurality of test tubes simultaneously, does not need to be manually placed in a solution, can be placed for a plurality of times at one time, saves time and ensures the safety of personnel.

Description

Cell culture recovery equipment

Technical Field

The invention relates to the technical field of cell recovery, in particular to cell culture recovery equipment.

Background

Cell recovery, a term of biology, refers to a process of recovering growth of cells by re-culturing after thawing the cells frozen in liquid nitrogen or in a refrigerator at-70 ℃.

In repeating the in-process to the cell test tube, mostly put into single test tube in the solution and unfreeze, put into solution back with the test tube, it is direct to its unfreeze through stable solution, the test tube directly takes out from the solution after unfreezing finishing, however, single operation, waste time, and manual operation, if irritate the liquid nitrogen in the test tube, lead to the test tube to break easily, and then probably take place to hinder the people accident, when the test tube unfreezes, test tube self absorbs the heat, the temperature of the peripheral solution of test tube is lower, the temperature that the cell unfreezes is comparatively accurate, fixed solution can lead to the cell when unfreezing not to absorb sufficient heat, and then the effect of unfreezing is poor.

Disclosure of Invention

The invention aims to provide cell culture resuscitation equipment, which solves the problems that in the prior art, time is wasted due to single operation, and in addition, due to manual operation, if liquid nitrogen is filled into a test tube, the test tube is easy to break, so that accidents of injury can occur.

In order to achieve the purpose, the invention adopts the following technical scheme:

the utility model provides a cell culture recovery equipment, includes the casing, and the top of casing is equipped with the upper cover, and the inside of casing is equipped with places the dish, places to be equipped with elevating system between dish and the casing, and the surface of placing the dish is equipped with a plurality of and is centrosymmetric distribution's test-tube clamp, and the bottom of casing is equipped with the landing leg, is equipped with actuating mechanism between landing leg and the casing.

Preferably, actuating mechanism includes the motor with landing leg fixed connection, and the bottom of casing is equipped with the ring gear, and the free end fixedly connected with of motor output shaft has the connecting rod of being connected with the ring gear, and the bottom of casing is equipped with the gear that is central symmetric distribution of a plurality of and ring gear engaged with, and the inside of casing is equipped with the stirring head that one end extends to the casing outside, stirring head and gear fixed connection.

Preferably, elevating system includes the slide rail that is the symmetric distribution with shells inner wall fixed connection, and the inside sliding connection of every slide rail has the slider, slider and place a set fixed connection, and the top of slider is equipped with the pull rod that one end runs through the casing.

Preferably, the bottom of slide rail is equipped with the limiting plate, limiting plate and slide rail fixed connection.

Preferably, the test tube clamp includes and places a set fixed connection's anchor clamps shell, the bottom fixedly connected with cross baffle of anchor clamps shell, and the inner wall of anchor clamps shell is equipped with a plurality of rows and is centrosymmetric distribution's block rubber, block rubber and anchor clamps shell fixed connection.

Preferably, the bottom of casing is equipped with collects the mechanism, collect the mechanism including with casing fixed connection's collection shell, collect the inside sliding connection of shell and have the closing plate, the below of closing plate be equipped with collect shell fixed connection's bottom.

Preferably, the inner wall of the bottom of the shell is provided with a horn-shaped groove.

Preferably, the top of casing is equipped with complementary unit, complementary unit include with casing fixed connection's sleeve, telescopic inside sliding connection has the slide bar, be equipped with the locking bolt between slide bar and the sleeve, the one end and the sleeve threaded connection of locking bolt, the locking bolt supports tight slide bar, the top of slide bar is equipped with the take-up (stock) pan, take-up (stock) pan and slide bar fixed connection, the top of take-up (stock) pan is equipped with a plurality of and is the material receiving hole of central symmetric distribution.

Preferably, the slide bar is square.

The invention has at least the following beneficial effects:

the test tube is inserted into the test tube clamp, the rubber block in the clamp shell clamps the outer side of the test tube, the cross baffle at the bottom of the clamp shell prevents the test tube from falling off when unfreezing, a small amount of liquid nitrogen is filled into part of the test tube, the test tube is broken in the heating process, the broken test tube is buffered by the clamp shell and the rubber block, the influence of the broken test tube on other test tubes is reduced, compared with the prior art, in the process of repeating the cell test tubes, the single test tube is mostly put into the solution to be thawed, however, the single operation wastes time, and the manual operation causes the test tube to be easily broken if liquid nitrogen is filled into the test tube, according to the scheme, a plurality of test tubes are clamped simultaneously by using the test tube clamp, the test tubes are not required to be placed into the solution manually, a plurality of test tubes can be placed at one time, the time is saved, and the safety of personnel is guaranteed;

starting equipment, the equipment heats the solution in the shell, after the temperature is enough, the upper cover is covered, a pull rod is pushed, the pull rod drives a sliding block to slide in a sliding groove, the sliding block drives a placing disc to enter the solution, the placing disc further drives the whole test tube clamp to be immersed in the solution, meanwhile, a motor drives a connecting rod to rotate, the connecting rod drives a gear ring to rotate, the gear ring further drives a gear to rotate, a gear drives a stirring head to rotate in the solution, the whole solution is in a flowing state, the uniformity of the temperature of each position of the solution is ensured, a test tube begins to be heated in the test tube clamp, compared with the prior art, after the test tube is placed in the solution, the test tube is directly unfrozen through stable solution, the test tube is directly taken out of the solution after unfreezing is finished, however, when the test tube is unfrozen, the self absorbs heat, fixed solution can lead to the cell not to absorb sufficient heat when unfreezing, and then the effect of unfreezing is poor, and the solution that this scheme use can move, when the cell unfreezes, moves the low temperature solution around the test tube to all the other positions, guarantees that the test tube periphery is in the temperature of unfreezing always, guarantees the stability of the temperature of unfreezing of test tube, and then the effect of unfreezing is better.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the description of the embodiments are briefly introduced below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without creative efforts.

FIG. 1 is a schematic view of an embodiment of the present invention;

FIG. 2 is a bottom view of FIG. 1;

FIG. 3 is a partial cross-sectional view of FIG. 1;

FIG. 4 is a cross-sectional view of a test tube fixture according to the present invention;

FIG. 5 is a partial cross-sectional view of FIG. 1;

FIG. 6 is a schematic diagram of a second embodiment of the present invention.

In the figure: 1. a housing; 2. an upper cover; 3. a support leg; 4. a drive mechanism; 40. a motor; 41. a connecting rod; 42. a toothed ring; 43. a gear; 44. a stirring head; 5. a lifting mechanism; 50. a slide rail; 51. a slider; 52. a limiting plate; 53. a pull rod; 6. placing a tray; 7. a test tube clamp; 70. a clamp housing; 71. a cross baffle plate; 72. a rubber block; 8. a collection mechanism; 80. a collection housing; 81. a sealing plate; 82. a bottom cover; 9. an auxiliary mechanism; 90. a sleeve; 91. a slide bar; 92. locking the bolt; 93. a take-up pan; 94. and a material receiving hole.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

Referring to fig. 1-5, in the first embodiment, a cell culture resuscitation device includes a housing 1, an upper cover 2 is disposed at the top of the housing 1, a placing tray 6 is disposed inside the housing 1, a lifting mechanism 5 is disposed between the placing tray 6 and the housing 1, a plurality of test tube clamps 7 are disposed on the surface of the placing tray 6, the test tube clamps are distributed in a central symmetry manner, support legs 3 are disposed at the bottom of the housing 1, and a driving mechanism 4 is disposed between the support legs 3 and the housing 1.

The driving mechanism 4 comprises a motor 40 fixedly connected with the supporting legs 3, a toothed ring 42 is arranged at the bottom of the shell 1, a connecting rod 41 connected with the toothed ring 42 is fixedly connected with the free end of an output shaft of the motor 40, the toothed ring 42 and the connecting rod 41 play a role in transmission, a plurality of gears 43 which are meshed with the toothed ring 42 and are distributed in a central symmetry manner are arranged at the bottom of the shell 1, a stirring head 44 with one end extending to the outer side of the shell 1 is arranged inside the shell 1, and the stirring head 44 is fixedly connected with the gears 43.

The lifting mechanism 5 comprises slide rails 50 which are fixedly connected with the inner wall of the shell 1 and are symmetrically distributed, a slide block 51 is connected to the inner part of each slide rail 50 in a sliding manner, the slide block 51 is fixedly connected with the placing disc 6, and a pull rod 53 with one end penetrating through the shell 1 is arranged at the top of the slide block 51.

The test tube clamp 7 comprises a clamp shell 70 fixedly connected with the placing plate 6, a cross baffle 71 is fixedly connected with the bottom of the clamp shell 70, a plurality of rows of rubber blocks 72 which are distributed in a central symmetry manner are arranged on the inner wall of the clamp shell 70, and the rubber blocks 72 are fixedly connected with the clamp shell 70

The scheme has the following working processes:

when the cell test tube is taken out of the liquid nitrogen, the test tube is inserted into the test tube clamp 7, the rubber block 72 in the clamp shell 70 clamps the outer side of the test tube, the cross baffle 71 at the bottom of the clamp shell 70 prevents the test tube from falling off during thawing, the equipment is started, the equipment heats the solution in the shell 1, after the temperature is enough, the upper cover 2 is covered, the pull rod 53 is pushed, the pull rod 53 drives the slide block 51 to slide in the chute, the slide block 51 drives the placing disc 6 to enter the solution, the placing disc 6 further drives the whole test tube clamp 7 to be immersed in the solution, meanwhile, the motor 40 is started, the motor 40 drives the connecting rod 41 to rotate, the connecting rod 41 drives the toothed ring 42 to rotate, the toothed ring 42 further drives the gear 43 to rotate, the gear 43 drives the stirring head 44 to rotate in the solution, so that the whole solution is in a flowing state, the uniformity of the temperature of each, pour into a small amount of liquid nitrogen in the partial test tube, take place to break at the heating process test tube, the test tube that breaks this moment receives anchor clamps shell 70 and the buffering of rubber block 72, reduces the influence of the test tube that breaks to other test tubes, reaches the back at heating time, opens upper cover 2, takes pull rod 53 out, pull rod 53 drives slider 51 and goes up slide, and then drives whole dish 6 that places and break away from solution, and the experimenter takes out intact test tube, and unfreezing of cell is accomplished.

According to the working process, the following steps are known:

the test tube is inserted into the test tube clamp 7, the rubber block 72 in the clamp shell 70 clamps the outer side of the test tube tightly, the cross baffle 71 at the bottom of the clamp shell 70 prevents the test tube from falling off when unfreezing, a small amount of liquid nitrogen is filled into a part of the test tube, the test tube is broken in the heating process, the broken test tube is buffered by the clamp shell 70 and the rubber block 72, the influence of the broken test tube on other test tubes is reduced, compared with the prior art, in the process of repeating cell test tubes, single test tube is mostly put into the solution to be unfrozen singly, however, single operation wastes time, manual operation is performed, if liquid nitrogen is filled into the test tube, the test tube is easy to break, and further, human injury accidents can occur, the scheme uses the test tube clamp 7 to clamp a plurality of test tubes simultaneously, manual placement into the solution is not needed, a plurality of test tubes can be placed at one, and the personnel safety is ensured;

starting equipment, the equipment heats the solution in the shell 1, after the temperature is enough, the upper cover 2 is covered, the pull rod 53 is pushed, the pull rod 53 drives the slide block 51 to slide in the chute, the slide block 51 drives the placing disc 6 to enter the solution, the placing disc 6 further drives the whole test tube clamp 7 to be immersed in the solution, meanwhile, the motor 40 is started, the motor 40 drives the connecting rod 41 to rotate, the connecting rod 41 drives the toothed ring 42 to rotate, the toothed ring 42 further drives the gear 43 to rotate, the gear 43 drives the stirring head 44 to rotate in the solution, so that the whole solution is in a flowing state, the uniformity of the temperature of each position of the solution is ensured, the test tube starts to be heated in the test tube clamp 7, compared with the prior art, after the test tube is placed in the solution, the test tube is directly unfrozen through the stable solution, the test tube is directly taken out of the solution after the unfree, the temperature of the peripheral solution of test tube is lower, and the temperature that the cell unfreezes is comparatively accurate, and fixed solution probably leads to the cell not to absorb sufficient heat when unfreezing, and then the effect of unfreezing is poor, and solution that this scheme used can move, during the cell unfreezes, moves the low temperature solution around the test tube to all the other positions, guarantees that the test tube periphery is in the temperature of unfreezing always, guarantees the stability of the temperature of unfreezing of test tube, and then the effect of unfreezing is better.

Further, the bottom of slide rail 50 is equipped with limiting plate 52, limiting plate 52 and slide rail 50 fixed connection, and when slider 51 slided on the surface of slide rail 50, limiting plate 52 blockked slider 51's downstream, prevented that test tube anchor clamps 7 and stirring head 44 from touching, protection test tube anchor clamps 7.

Further, the bottom of the shell 1 is provided with a collecting mechanism 8, the collecting mechanism 8 comprises a collecting shell 80 fixedly connected with the shell 1, a sealing plate 81 is slidably connected inside the collecting shell 80, a bottom cover 82 fixedly connected with the collecting shell 80 is arranged below the sealing plate 81, if the test tube in the test tube clamp 7 is broken due to heating nitrogen, the broken slag of the test tube rotates along with the stirring head 44, the broken slag is directly collected on the inner wall of the bottom of the shell 1 along with the flow of the solution, the broken slag falls along the collecting shell 80, then the sealing plate 81 is pulled, the sealing plate 81 is opened, the broken slag continues to fall, then the sealing plate 81 is closed, the bottom cover 82 is opened, the broken slag is poured out, the impurities in the solution are reduced by the collecting mechanism 8 at the bottom, the heating effect of the solution is better, and prevents the slag from accumulating to damage the inner wall of the shell 1, thereby being more convenient for unfreezing the cells.

Furthermore, the bottom inner wall of casing 1 is equipped with the recess that is loudspeaker form, when the inside glass disintegrating slag that has the test tube that bursts of heating solution, directly collects the disintegrating slag through the recess of loudspeaker form, collects and discharges through collecting mechanism 8.

According to the above embodiment, please refer to fig. 6, embodiment two, the top of the housing 1 is provided with an auxiliary mechanism 9, the auxiliary mechanism 9 includes a sleeve 90 fixedly connected with the housing 1, a slide rod 92 is slidably connected inside the sleeve 90, a locking bolt 92 is arranged between the slide rod 92 and the sleeve 90, one end of the locking bolt 92 is in threaded connection with the sleeve 90, the locking bolt 92 abuts against the slide rod 92, a receiving tray 93 is arranged on the top of the slide rod 92, the receiving tray 93 is fixedly connected with the slide rod 91, the top of the receiving tray 93 is provided with a plurality of receiving holes 94 which are distributed in central symmetry, the slide rod 91 is square, when cells in a batch need to be thawed in the test tubes, the cell test tubes can be placed in the receiving holes 94 of the receiving tray 93 first, after other cells are thawed, the cell test tubes on the top of the receiving tray 94 can be placed in the test tube clamp 7 quickly, more conveniently heat the test tube, further the person of facilitating the use unfreezes it.

To sum up, the scheme uses the solution which can move, during the cell thawing period, the low-temperature solution around the test tube is moved to other positions, the circumference of the test tube is ensured to be always in the thawing temperature, the stability of the thawing temperature of the test tube is ensured, the thawing effect is better, the inner wall of the bottom of the shell 1 is provided with a trumpet-shaped groove, when the cracked broken glass slag of the test tube exists in the heating solution, the broken glass slag is directly collected through the horn-shaped groove, when the cells in the test tubes need to be thawed and the batch of cells need to be thawed, the cell test tubes can be placed in the receiving holes 94 of the receiving tray 93 first, after other cells finish unfreezing, can be rapid place the cell test tube at take-up (94) top in test tube holder 7, more conveniently heat the test tube, further the person of facilitating the use unfreezes it.

The foregoing shows and describes the general principles, essential features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are merely illustrative of the principles of the invention, but that various changes and modifications may be made without departing from the spirit and scope of the invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims

1. The utility model provides a cell culture recovery equipment, includes casing (1), its characterized in that, the top of casing (1) is equipped with upper cover (2), the inside of casing (1) is equipped with places dish (6), it is equipped with elevating system (5) to place between dish (6) and casing (1), the surface of placing dish (6) is equipped with test tube anchor clamps (7) that a plurality of is central symmetric distribution, the bottom of casing (1) is equipped with landing leg (3), be equipped with actuating mechanism (4) between landing leg (3) and casing (1).

2. The cell culture resuscitation device according to claim 1, wherein the driving mechanism (4) comprises a motor (40) fixedly connected with the support leg (3), a toothed ring (42) is arranged at the bottom of the housing (1), a connecting rod (41) connected with the toothed ring (42) is fixedly connected with the free end of an output shaft of the motor (40), a plurality of gears (43) which are meshed with the toothed ring (42) and are distributed in a central symmetry manner are arranged at the bottom of the housing (1), a stirring head (44) with one end extending to the outer side of the housing (1) is arranged in the housing (1), and the stirring head (44) is fixedly connected with the gears (43).

3. The cell culture resuscitation device according to claim 1, wherein the lifting mechanism (5) comprises symmetrically distributed slide rails (50) fixedly connected with the inner wall of the housing (1), a slide block (51) is slidably connected inside each slide rail (50), the slide block (51) is fixedly connected with the placing disc (6), and a pull rod (53) with one end penetrating through the housing (1) is arranged at the top of the slide block (51).

4. The cell culture resuscitation device according to claim 3, wherein a limiting plate (52) is disposed at the bottom of the sliding rail (50), and the limiting plate (52) is fixedly connected to the sliding rail (50).

5. The cell culture resuscitation device according to claim 1, wherein the test tube holder (7) comprises a holder housing (70) fixedly connected to the placing tray (6), a cross-shaped baffle (71) is fixedly connected to the bottom of the holder housing (70), a plurality of rows of rubber blocks (72) are arranged on the inner wall of the holder housing (70) and are distributed in a central symmetry manner, and the rubber blocks (72) are fixedly connected to the holder housing (70).

6. The cell culture resuscitation device according to claim 1, wherein the bottom of the housing (1) is provided with a collection mechanism (8), the collection mechanism (8) comprises a collection housing (80) fixedly connected with the housing (1), a sealing plate (81) is slidably connected to the interior of the collection housing (80), and a bottom cover (82) fixedly connected with the collection housing (80) is arranged below the sealing plate (81).

7. Cell culture resuscitation device according to claim 1, wherein the bottom inner wall of the housing (1) is provided with a trumpet-shaped groove.

8. The cell culture resuscitation device according to claim 1, wherein an auxiliary mechanism (9) is arranged at the top of the housing (1), the auxiliary mechanism (9) comprises a sleeve (90) fixedly connected with the housing (1), a slide rod (92) is slidably connected inside the sleeve (90), a locking bolt (92) is arranged between the slide rod (92) and the sleeve (90), one end of the locking bolt (92) is in threaded connection with the sleeve (90), the locking bolt (92) abuts against the slide rod (92), a material receiving disc (93) is arranged at the top of the slide rod (92), the material receiving disc (93) is fixedly connected with the slide rod (91), and a plurality of material receiving holes (94) are symmetrically distributed in the center are arranged at the top of the material receiving disc (93).

9. The cell culture resuscitation device according to claim 8, wherein the slide bar (91) is square.