CN1115335A - Method for prodn. of gallic acid by fermentation process - Google Patents
Method for prodn. of gallic acid by fermentation process Download PDFInfo
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- CN1115335A CN1115335A CN 94107730 CN94107730A CN1115335A CN 1115335 A CN1115335 A CN 1115335A CN 94107730 CN94107730 CN 94107730 CN 94107730 A CN94107730 A CN 94107730A CN 1115335 A CN1115335 A CN 1115335A
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- gallic acid
- raw material
- hours
- tannin
- fermentative production
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The fermentation process for production of gallic acid includes immersing microbes after slant culture in solution of tannic acid for inducing for a while, immersing and washing spores with sterile soft water, breaking raw material and controlling its granularity, immersing the raw material in water and fermentation, and features, no environmental pollution and high recovery rate.
Description
The present invention relates to a kind of method of fermentative Production gallic acid.
At present, containing hydrolysis tannin plant material with Turkey-galls, Ta La or other produces the method for gallic acid and mainly contains hydrolysis method and microbe fermentation method, the shortcoming of hydrolysis method is that the transformation efficiency and the rate of recovery are all low, and the waste water that its production is produced also can produce certain environmental pollution; Microbe fermentation method of the prior art, as number of patent application is 93107994.2, name is called the application for a patent for invention of the technology of preparation of gallic acid by using enzyme, its disclosed processing method, though its transformation efficiency and the rate of recovery are all than hydrolysis method height, but owing to require to add alkali control pH value in its technology, so just increased the complicacy of production cost and technology, in addition in the prior art for enlarging when producing tannase inducing with seed with the microorganism behind the slant culture, owing to do not note adopting at a certain temperature pure tannase solution soaking microorganism in its technology, therefore generate other enzyme easily, do not note controlling raw material granularity in addition in the prior art, if raw material granularity too slightly is unfavorable for the leaching of tannin molecule, if raw material granularity too detailed rules and regulations can make tannin molecular diffusion condition destroyed and produce the powder conglomeration again, cause fluid flow resistance to strengthen, insolubles is increased, and these shortcomings of the prior art have all reduced the output of tannase and have reduced the rate of recovery of gallic acid.
The purpose of this invention is to provide and a kind ofly need not add alkali, rationally soak microorganism and control raw material granularity method with the fermentative Production gallic acid that improves gallic acid output.
The object of the present invention is achieved like this: it comprises that the microorganism behind the employing slant culture makes tannase, make Turkey-galls, Ta La or other contain tannin hydrolysis in the hydrolysis tannin plant material with tannase, with the tannin that is hydrolyzed after filtration, concentrate, crystallization, decolouring, drying and make gallic acid, microorganism behind the slant culture is soaked through 2%～8% pure tannic acid solution, under 28 ℃～34 ℃ temperature, induced the spore after soaking with aseptic soft water washing again 2～5 times 12 hours～24 hours; With raw material crushing, its granularity is controlled at 2～8mm, sterilizes then, also soaks raw material 8 hours～12 hours with the water yield that accounts for 7～10 times of raw material weights under 60 ℃～85 ℃ temperature; With 80 ℃～95 ℃ hot water washery slags and wash-out look gac, with the frozen water wash crystallization product below 4 ℃, to reduce the loss in the aftertreatment.
Compared with prior art, production cost of the present invention is low, and is free from environmental pollution, the rate of recovery height of raw material.
Accompanying drawing 1 is a process flow sheet of the present invention.
Go on foot the explanation embodiments of the invention below in conjunction with accompanying drawing nearly:
The proportioning of slant medium is as follows:
Distilled water 1000ml
NaNO
3 3g
K
2HPO
4 1g
MgSO
47H
2O 0.5g
Kd 0.5g
TeSO
4?7H
2O 0.01g
Sucrose 30g
Agar 15g
Pressurize 15 pounds sterilization in 30 minutes.
On the inclined-plane after the sterilization, carry out microbial inoculant.
Microorganism behind the slant culture is soaked through 2%～8% pure tannic acid solution, under 28 ℃～34 ℃ temperature, induced 12 hours～24 hours, again with the spore after the aseptic soft water washing immersion 2～5 times; Put it into afterwards and carry out the seed enlarged culturing in the following prescription:
NaNO
3 2.5g
K
2HPO
4 0.18g
μgSO
4?7H
2O 0.25g
Sucrose 15g
Tannin 50g
Yeast 0.5g
Water 1000ml
With raw material crushing, its granularity is controlled at about 5mm, uses the water yield of 7～10 times of raw material weights then, under 60 ℃～85 ℃ temperature, soak raw material 8 little right～12 hours; With 80 ℃～95 ℃ hot water washery slags and wash-out look gac, with the frozen water wash crystallization product below 4 ℃, to reduce the gallic acid loss in the aftertreatment.
Claims (3)
1, a kind of method of fermentative Production gallic acid, it comprises that the microorganism behind the employing slant culture makes tannase, make Turkey-galls, Ta La or other contain tannin hydrolysis in the hydrolysis tannin plant material with tannase, with the tannin that is hydrolyzed after filtration, concentrate, crystallization, decolouring, drying and make gallic acid, it is characterized in that: the microorganism behind the slant culture is soaked through 2%～8% pure tannic acid solution, under 28 ℃～34 ℃ temperature, induced the spore after soaking with aseptic soft water washing again 2～5 times 12 hours～24 hours.
2, the method for fermentative Production gallic acid according to claim 1, it is characterized in that: with raw material crushing, its granularity is controlled at 2～8mm, sterilizes then, also soaks raw material 8 hours～12 hours with the water yield that accounts for 7～10 times of raw material weights under 60 ℃～85 ℃ temperature.
3, the method for fermentative Production gallic acid according to claim 1 is characterized in that: with 80 ℃～95 ℃ hot water washery slags and wash-out look gac; With 4 ° of frozen water wash crystallization products below the C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 94107730 CN1115335A (en) | 1994-07-16 | 1994-07-16 | Method for prodn. of gallic acid by fermentation process |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 94107730 CN1115335A (en) | 1994-07-16 | 1994-07-16 | Method for prodn. of gallic acid by fermentation process |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1115335A true CN1115335A (en) | 1996-01-24 |
Family
ID=5033191
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 94107730 Pending CN1115335A (en) | 1994-07-16 | 1994-07-16 | Method for prodn. of gallic acid by fermentation process |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1115335A (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100436589C (en) * | 2001-06-22 | 2008-11-26 | 印度技术研究院 | Process for preparation of gallic acid by co-culture |
| CN101280279B (en) * | 2008-04-09 | 2010-06-02 | 东北林业大学 | Phomopsis capable of producing gallic acid |
| CN101864459A (en) * | 2010-05-07 | 2010-10-20 | 遵义市倍缘化工有限责任公司 | Method for preparing gallic acid |
| CN102443614A (en) * | 2011-11-10 | 2012-05-09 | 湖南农业大学 | Method for separating and purifying epigallocatechin (EGC) monomer |
| CN102643871A (en) * | 2012-04-17 | 2012-08-22 | 昆明怡普康植物化学有限公司 | Preparation method of high-purity gallic acid |
| CN103589758A (en) * | 2013-11-12 | 2014-02-19 | 遵义市倍缘化工有限责任公司 | Method for preparing gallic acid by using gallnut tannin as raw material and utilizing fermentation separation coupling technology |
| CN103893392A (en) * | 2012-12-25 | 2014-07-02 | 杭州桐君堂生物科技有限公司 | Method for improving gallic acid content of Chinese gall leaven |
| WO2022268194A1 (en) * | 2021-06-24 | 2022-12-29 | 南京百斯杰生物工程有限公司 | Application of tannase in preparation of gallic acid |
-
1994
- 1994-07-16 CN CN 94107730 patent/CN1115335A/en active Pending
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100436589C (en) * | 2001-06-22 | 2008-11-26 | 印度技术研究院 | Process for preparation of gallic acid by co-culture |
| CN101280279B (en) * | 2008-04-09 | 2010-06-02 | 东北林业大学 | Phomopsis capable of producing gallic acid |
| CN101864459A (en) * | 2010-05-07 | 2010-10-20 | 遵义市倍缘化工有限责任公司 | Method for preparing gallic acid |
| CN102443614A (en) * | 2011-11-10 | 2012-05-09 | 湖南农业大学 | Method for separating and purifying epigallocatechin (EGC) monomer |
| CN102643871A (en) * | 2012-04-17 | 2012-08-22 | 昆明怡普康植物化学有限公司 | Preparation method of high-purity gallic acid |
| CN102643871B (en) * | 2012-04-17 | 2014-02-26 | 昆明怡普康植物化学有限公司 | Preparation method of high-purity gallic acid |
| CN103893392A (en) * | 2012-12-25 | 2014-07-02 | 杭州桐君堂生物科技有限公司 | Method for improving gallic acid content of Chinese gall leaven |
| CN103589758A (en) * | 2013-11-12 | 2014-02-19 | 遵义市倍缘化工有限责任公司 | Method for preparing gallic acid by using gallnut tannin as raw material and utilizing fermentation separation coupling technology |
| CN103589758B (en) * | 2013-11-12 | 2015-07-22 | 遵义市倍缘化工有限责任公司 | Method for preparing gallic acid by using gallnut tannin as raw material and utilizing fermentation separation coupling technology |
| WO2022268194A1 (en) * | 2021-06-24 | 2022-12-29 | 南京百斯杰生物工程有限公司 | Application of tannase in preparation of gallic acid |
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| C01 | Deemed withdrawal of patent application (patent law 1993) | ||
| WD01 | Invention patent application deemed withdrawn after publication |