CN111533266A - Preparation method of biological flocculant and raw material treatment device - Google Patents

Preparation method of biological flocculant and raw material treatment device Download PDF

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CN111533266A
CN111533266A CN202010269382.3A CN202010269382A CN111533266A CN 111533266 A CN111533266 A CN 111533266A CN 202010269382 A CN202010269382 A CN 202010269382A CN 111533266 A CN111533266 A CN 111533266A
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biological
solution
domestication
raw material
biological flocculant
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李锋
张饮江
杜佳沐
姬芬
张宁
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Shanghai Zhonghui Water Ecological Technology Co ltd
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Shanghai Zhonghui Water Ecological Technology Co ltd
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    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/32Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B02CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
    • B02CCRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
    • B02C23/00Auxiliary methods or auxiliary devices or accessories specially adapted for crushing or disintegrating not provided for in preceding groups or not specially adapted to apparatus covered by a single preceding group
    • B02C23/08Separating or sorting of material, associated with crushing or disintegrating
    • B02C23/16Separating or sorting of material, associated with crushing or disintegrating with separator defining termination of crushing or disintegrating zone, e.g. screen denying egress of oversize material
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B02CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
    • B02CCRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
    • B02C4/00Crushing or disintegrating by roller mills
    • B02C4/02Crushing or disintegrating by roller mills with two or more rollers
    • B02C4/08Crushing or disintegrating by roller mills with two or more rollers with co-operating corrugated or toothed crushing-rollers
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B02CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
    • B02CCRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
    • B02C4/00Crushing or disintegrating by roller mills
    • B02C4/28Details
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00Treatment of water, waste water, or sewage
    • C02F1/52Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/36Adaptation or attenuation of cells
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B02CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
    • B02CCRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
    • B02C23/00Auxiliary methods or auxiliary devices or accessories specially adapted for crushing or disintegrating not provided for in preceding groups or not specially adapted to apparatus covered by a single preceding group
    • B02C23/08Separating or sorting of material, associated with crushing or disintegrating
    • B02C23/16Separating or sorting of material, associated with crushing or disintegrating with separator defining termination of crushing or disintegrating zone, e.g. screen denying egress of oversize material
    • B02C2023/165Screen denying egress of oversize material
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B02CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
    • B02CCRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
    • B02C2201/00Codes relating to disintegrating devices adapted for specific materials
    • B02C2201/06Codes relating to disintegrating devices adapted for specific materials for garbage, waste or sewage

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  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Water Supply & Treatment (AREA)
  • Environmental & Geological Engineering (AREA)
  • Hydrology & Water Resources (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Botany (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Cell Biology (AREA)
  • Separation Of Suspended Particles By Flocculating Agents (AREA)
  • Treatment Of Sludge (AREA)
  • Processing Of Solid Wastes (AREA)

Abstract

The invention discloses a preparation method of a biological flocculant and a raw material treatment device, belonging to the technical field of biological flocculants; the preparation method comprises the following steps: treating raw materials; preparing kitchen waste microbial liquid; preparing fermentation liquor; extracting the biological flocculant; the raw material processing apparatus is used for principle processing. The preparation method of the biological flocculant is scientific and reasonable in design, the raw material treatment device is used for treating the domestic kitchen garbage, inorganic matters and grease in the domestic kitchen garbage are removed, the domestic kitchen garbage is mixed with domestic sewage for precipitation, suspension liquid is removed for domestication and culture, bacterial liquid can be obtained, the raw materials are cheap and easy to obtain, the source is wide, the preparation cost of the bacterial liquid of the microbial flocculant is greatly reduced, the resource utilization of the domestic kitchen garbage is realized, and the preparation method is simple and feasible.

Description

Preparation method of biological flocculant and raw material treatment device
Technical Field
The invention belongs to the technical field of biological flocculants, and particularly relates to a preparation method of a biological flocculant and a raw material processing device.
Background
With the increasing severity of the problem of water environment pollution and the increasing awareness of environmental protection, the development of a novel green flocculant which is efficient, safe, free of secondary pollution, available in raw materials and low in price has become the demand of the times due to certain defects of the traditional biological flocculant. The microbial flocculant is a secondary metabolite which is generated by microorganisms and has flocculation activity, and the main components of the microbial flocculant comprise high molecular substances such as glycoprotein, mucopolysaccharide, cellulose, DNA and the like. The bioflocculant is a typical environment-friendly functional material, adapts to the concept of the current sustainable development, and has important significance on human health and environmental protection.
The kitchen waste refers to food waste and food residues in families, schools, institutions, public dining halls and catering industries and is a main component of municipal domestic waste. At present, kitchen wastes become a public nuisance, and if the kitchen wastes are not properly treated, the kitchen wastes not only harm the health of people, but also seriously pollute the environment. The kitchen wastes can be simply divided into diluted domestic kitchen wastes, hard domestic kitchen wastes and mixed domestic kitchen wastes between the diluted domestic kitchen wastes and the hard domestic kitchen wastes according to the solid-liquid ratio. Most kitchen wastes generated in different occasions are one of three types, and most of the types of the kitchen wastes generated in fixed occasions are consistent due to the environment.
In the prior art, the preparation method of the biological flocculant has the problems of high preparation cost of bacterial liquid, poor flocculation effect, poor decoloration and the like.
Disclosure of Invention
The invention aims to overcome at least one problem existing in the prior art and provide a preparation method of a biological flocculant and a raw material treatment device.
In order to achieve the technical purpose and achieve the technical effect, the invention is realized by the following technical scheme:
a preparation method of a biological flocculant comprises the following steps:
step one, raw material treatment: putting the domestic kitchen garbage into a raw material treatment device, primarily crushing the domestic kitchen garbage by the raw material treatment device, removing inorganic matters and grease, mixing the domestic kitchen garbage with domestic sewage for precipitation to obtain a suspension, adjusting the pH value to 7-7.5 by acid liquor or alkali liquor, and placing the suspension on a shaking table for domestication culture;
step two, preparing kitchen waste microorganism bacterium liquid: taking starch wastewater subjected to high-temperature sterilization as a culture medium, adding water for the first time to dilute the starch wastewater to 20% of the original concentration as the culture medium, inoculating 10% of suspension, and culturing for 48-72h to obtain a first domestication solution; adding water for the second time to dilute to 40% of the original concentration to be used as a culture medium, inoculating 10% of the first domestication solution for domestication to obtain a second domestication solution; diluting with water for the third time to 60% of the original concentration as culture medium, inoculating 10% of the second acclimation solution, and acclimating to obtain a third acclimation solution; adding water for the fourth time to dilute to 80% of the original concentration to be used as a culture medium, inoculating 10% of the third acclimation solution for acclimation, and obtaining kitchen waste microorganism solution;
step three, preparation of fermentation liquor: cooling the starch wastewater to room temperature after high-temperature sterilization, inoculating a proper amount of the kitchen waste microorganism liquid, and fermenting for 40-50h at the temperature of 28-35 ℃ at the temperature of 100-200r/min to obtain fermentation liquid;
step four, extracting the biological flocculant: centrifuging the fermentation liquor to obtain supernatant and precipitate, detecting the pH value of the precipitate, adjusting the pH value to 6-10 with a proper amount of sodium hydroxide solution, and hydrolyzing to obtain hydrolysate; performing ultrasonic crushing and centrifugation on the hydrolysate to obtain secondary supernatant and precipitate, combining the primary supernatant and the secondary supernatant, adding a carbon source, auxiliary materials and a biological activating enzyme, then concentrating to 10-15% of the original volume by adopting an evaporator at the temperature of 30-45 ℃, and performing vacuum drying to obtain biological flocculant powder; and dissolving the biological flocculant powder in water to prepare a solution, thereby obtaining the biological flocculant.
Further, in the first step, the rotation speed of the shaking table for the acclimatization culture is 100-.
Further, in the third step, the volume of the inoculated kitchen waste microorganism liquid is 7-10% of the volume of the starch wastewater.
Further, in the fourth step, the fermentation liquor is centrifuged at 3000r/min for 10 min.
Further, in the fourth step, the mass concentration of the sodium hydroxide solution is 0.3 mol/L; the hydrolysis time is 40-60 minutes; the drying temperature of the vacuum drying is 100-.
Further, in the fourth step, the mass ratio of the supernatant to the carbon source to the bioactivator is 6:2: 2.
Further, in the fourth step, the carbon source is glucose or sodium acetate; the biological activating enzyme is catalase or amylase; the adjuvants are one or more of Gypsum Fibrosum, diatomaceous earth, and Kaolin.
A raw material processing device of a bioflocculant comprises a movable base, wherein a processing box is fixed at the upper end of the movable base, an input pipe for inputting household kitchen garbage is arranged at the top end of the processing box, a conical hopper-shaped partition plate is arranged in an inner cavity of the processing box, a crushing chamber is arranged above the conical hopper-shaped partition plate, double crushing rollers are arranged in the crushing chamber, the center of the conical hopper-shaped partition plate is communicated with the upper side of a screening barrel through a guide pipe, the screening barrel is obliquely designed, the high-level end of the screening barrel is positioned outside the processing box, the low-level end of the screening barrel is positioned inside the processing box, a spiral conveying shaft for screening is arranged in the screening barrel, the top end of the spiral conveying shaft is fixedly connected with an output shaft of a screening motor through a coupler, the screening motor is fixed on the outer wall of the processing box through a bracket, and the lower side of the screening barrel, which is positioned at the high-level, the screening barrel is located at the lower side of the low-order end and is communicated with the input end of the three-phase centrifuge through a liquid discharge pipe, an oil discharge pipe of the three-phase centrifuge is communicated with the oil storage barrel, and the liquid discharge pipe of the three-phase centrifuge is communicated with the collection barrel.
Furthermore, the spiral conveying shaft is composed of a hollow shaft and a spiral blade fixed on the outer side of the hollow shaft, and at least one of the hollow shaft and the spiral blade is uniformly provided with a filtering hole.
The invention has the beneficial effects that:
1. the preparation method of the biological flocculant is scientific and reasonable in design, the raw material treatment device is used for treating the domestic kitchen garbage, inorganic matters and grease in the domestic kitchen garbage are removed, the domestic kitchen garbage is mixed with domestic sewage for precipitation, suspension liquid is removed for domestication and culture, bacterial liquid can be obtained, the raw materials are cheap and easy to obtain, the source is wide, the preparation cost of the bacterial liquid of the microbial flocculant is greatly reduced, the resource utilization of the domestic kitchen garbage is realized, and the preparation method is simple and feasible.
2. The biological flocculant raw material treatment device is reasonable in structural design, three conveying modes with different rotating speeds are designed for conveying inorganic matters after different household kitchen waste is crushed, dilute type household kitchen waste is conveyed in a low-speed rotating mode, hard type household kitchen waste is conveyed in a high-speed rotating mode, and mixed type household kitchen waste between the dilute type and the hard type is conveyed in a medium-speed rotating mode.
Of course, it is not necessary for any one product that embodies the invention to achieve all of the above advantages simultaneously.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a block flow diagram of a method of preparing a bioflocculant according to the present invention;
FIG. 2 is a schematic diagram of the structure of a biological flocculant raw material treatment apparatus according to the present invention;
FIG. 3 is a schematic structural view of a fifth embodiment of the present invention;
FIG. 4 is a schematic structural view of a conveying screw according to a sixth embodiment of the present invention;
fig. 5 is a schematic structural view of a conveying screw shaft in the seventh embodiment of the present invention.
In the drawings, the components represented by the respective reference numerals are listed below:
1-a movable base, 2-a treatment box, 3-an input pipe, 4-a cone-shaped partition plate, 5-a crushing chamber, 6-double crushing rollers, 7-a guide pipe, 8-a screening cylinder, 9-a spiral conveying shaft, 901-a hollow shaft, 902-a spiral blade, 10-a screening motor, 11-a bracket, 12-a solid discharge pipe, 13-an inorganic matter storage cylinder, 14-a liquid discharge pipe, 15-a three-phase centrifuge, 16-an oil storage cylinder and 17-a collection cylinder.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example one
This embodiment is a method for preparing a bioflocculant, as shown in fig. 1, including the following steps:
step one, raw material treatment: putting the domestic kitchen garbage into a raw material treatment device, primarily crushing the domestic kitchen garbage by the raw material treatment device, removing inorganic matters and grease, mixing the domestic kitchen garbage with domestic sewage for precipitation to obtain a suspension, adjusting the pH value to 7-7.5 by acid liquor or alkali liquor, and placing the suspension on a shaking table for domestication culture; the rotating speed of a shaking table for domestication culture is 100-.
Step two, preparing kitchen waste microorganism bacterium liquid: taking starch wastewater subjected to high-temperature sterilization as a culture medium, adding water for the first time to dilute the starch wastewater to 20% of the original concentration as the culture medium, inoculating 10% of suspension, and culturing for 48-72h to obtain a first domestication solution; adding water for the second time to dilute to 40% of the original concentration to be used as a culture medium, inoculating 10% of the first domestication solution for domestication to obtain a second domestication solution; diluting with water for the third time to 60% of the original concentration as culture medium, inoculating 10% of the second acclimation solution, and acclimating to obtain a third acclimation solution; adding water for the fourth time to dilute to 80% of the original concentration to be used as a culture medium, inoculating 10% of the third acclimation solution for acclimation, and obtaining kitchen waste microorganism solution;
step three, preparation of fermentation liquor: cooling the starch wastewater to room temperature after high-temperature sterilization, and inoculating kitchen waste microorganism liquid, wherein the volume of the inoculated kitchen waste microorganism liquid is 7-10% of the volume of the starch wastewater; fermenting at 28-35 deg.C for 40-50h at 200r/min for 100-;
step four, extracting the biological flocculant: centrifuging the fermentation liquor for 10min at 3000r/min to obtain supernatant and precipitate, detecting the pH value of the precipitate, adjusting the pH value to 6-10 with a proper amount of sodium hydroxide solution with the mass concentration of 0.3mol/L, hydrolyzing, and hydrolyzing for 40-60 min to obtain hydrolysate;
and (3) carrying out ultrasonic crushing and centrifugation on the hydrolysate to obtain secondary supernatant and precipitate, combining the primary supernatant and the secondary supernatant, and adding a carbon source, auxiliary materials and a biological activating enzyme, wherein the mass ratio of the supernatant to the carbon source to the biological activating enzyme is 6:2: 2. The carbon source is glucose; the biological activating enzyme is catalase; the auxiliary material is gypsum.
Then concentrating to 10% of the original volume by adopting an evaporator at the temperature of 30 ℃, and then drying in vacuum at the drying temperature of 100 ℃ for 0.5h to obtain a biological flocculant powder; the biological flocculant powder is dissolved in water to prepare a solution, so that the biological flocculant is obtained, and has the advantages of good flocculation effect and high decoloration.
Example two
The present example is substantially the same as the first example, except that in the fourth step, the carbon source is sodium acetate; the biological activating enzyme is catalase; the auxiliary material is a mixture of diatomite and kaolin.
Then concentrating to 15% of the original volume by adopting an evaporator at the temperature of 45 ℃, and then drying in vacuum at the drying temperature of 110 ℃ for 1.5h to obtain a biological flocculant powder; the biological flocculant powder is dissolved in water to prepare a solution, so that the biological flocculant is obtained, and has the advantages of good flocculation effect and high decoloration.
EXAMPLE III
This example is substantially the same as the first example, except that in the fourth step, the carbon source is glucose; the biological activating enzyme is amylase; the auxiliary material is kaolin.
Then concentrating to 12% of the original volume by adopting an evaporator at 33 ℃, and then drying in vacuum at 105 ℃ for 0.8h to obtain a biological flocculant powder; the biological flocculant powder is dissolved in water to prepare a solution, so that the biological flocculant is obtained, and has the advantages of good flocculation effect and high decoloration.
Example four
The present example is substantially the same as the first example, except that in the fourth step, the carbon source is sodium acetate; the biological activating enzyme is catalase; the auxiliary material is gypsum.
Then concentrating to 13% of the original volume by adopting an evaporator at 40 ℃, and then drying in vacuum at the drying temperature of 108 ℃ for 1.2h to obtain a biological flocculant powder; the biological flocculant powder is dissolved in water to prepare a solution, so that the biological flocculant is obtained, and has the advantages of good flocculation effect and high decoloration.
EXAMPLE five
The embodiment is a raw material processing device of a biological flocculant, as shown in fig. 2, and comprises a movable base 1, a processing box 2 is fixed at the upper end of the movable base 1, an input pipe 3 for inputting household kitchen garbage is arranged at the top end of the processing box 2, a conical hopper-shaped partition plate 4 is arranged in the inner cavity of the processing box 2, a crushing chamber 5 is arranged above the conical hopper-shaped partition plate 4, and double crushing rollers 6 are arranged in the crushing chamber 5. The center department of awl hopper-shaped baffle 4 is through honeycomb duct 7 and the upside intercommunication of a screening section of thick bamboo 8, and 8 slope designs of a screening section of thick bamboo, and its high-order end is located the outside of handling case 2, and the low level end is located the inside of handling case 2. The screening cylinder 8 is internally provided with a spiral conveying shaft 9 for screening, the top end of the spiral conveying shaft 9 is fixedly connected with an output shaft of a screening motor 10 through a coupler, and the screening motor 10 is fixed on the outer wall of the treatment box 2 through a support 11. The screening section of thick bamboo 8 is located the downside of high-order end and has inorganic matter storage tube 13 through row solid pipe 12 intercommunication, and screening section of thick bamboo 8 is located the downside of low-order end and passes through fluid-discharge tube 14 and three-phase centrifuge 15's input intercommunication, and three-phase centrifuge 15's oil extraction pipe and oil storage tube 16 intercommunication, three-phase centrifuge 15's fluid-discharge tube (containing clear liquid pipe and heavy liquid pipe) and collection section of thick bamboo 17 intercommunication. The oil storage cylinder 16 and the collecting cylinder 17 are respectively provided with a material pumping pipe.
In this embodiment, as shown in fig. 3, the spiral conveying shaft 9 is composed of a hollow shaft 901 and spiral blades 902 fixed on the outer side of the hollow shaft 901, the bottom end of the hollow shaft 901 is rotationally limited in the positioning block, the spiral blades 902 are uniformly distributed with filter holes, and the spiral blades 902 are equidistant spiral blades and suitable for medium-speed rotary conveying.
EXAMPLE six
The structure and application of the embodiment are substantially the same as those of the fifth embodiment, and the difference is that, as shown in fig. 4, the spiral conveying shaft 9 is composed of a hollow shaft 901 and spiral blades 902 fixed on the outer side of the hollow shaft 901, filtering holes are uniformly distributed on the hollow shaft 901, the bottom end of the hollow shaft 901 is an opening, and the diameter of the spiral blades 902 is equal to the diameter of the inner cavity of the sieving cylinder 8; the spiral leaves 902 are equidistant solid spiral leaves and are suitable for low-speed rotary conveying.
EXAMPLE seven
The structure and application of the embodiment are substantially the same as those of the fifth embodiment, and the difference is that, as shown in fig. 5, the spiral conveying shaft 9 is composed of a hollow shaft 901 and spiral blades 902 fixed on the outer side of the hollow shaft 901, filtering holes are uniformly distributed on the hollow shaft 901 and the spiral blades 902, the bottom end of the hollow shaft 901 is an opening, and the diameter of the spiral blades 902 is equal to the diameter of the inner cavity of the sieving cylinder 8; the screw blade 902 is a torque-variable screw blade, the screw pitch of which is gradually reduced from bottom to top, and is suitable for high-speed rotation conveying.
The preferred embodiments of the invention disclosed above are intended to be illustrative only. The preferred embodiments are not intended to be exhaustive or to limit the invention to the precise embodiments disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, to thereby enable others skilled in the art to best utilize the invention. The invention is limited only by the claims and their full scope and equivalents.

Claims (9)

1. The preparation method of the biological flocculant is characterized by comprising the following steps of:
step one, raw material treatment: putting the domestic kitchen garbage into a raw material treatment device, primarily crushing the domestic kitchen garbage by the raw material treatment device, removing inorganic matters and grease, mixing the domestic kitchen garbage with domestic sewage for precipitation to obtain a suspension, adjusting the pH value to 7-7.5 by acid liquor or alkali liquor, and placing the suspension on a shaking table for domestication culture;
step two, preparing kitchen waste microorganism bacterium liquid: taking starch wastewater subjected to high-temperature sterilization as a culture medium, adding water for the first time to dilute the starch wastewater to 20% of the original concentration as the culture medium, inoculating 10% of suspension, and culturing for 48-72h to obtain a first domestication solution; adding water for the second time to dilute to 40% of the original concentration to be used as a culture medium, inoculating 10% of the first domestication solution for domestication to obtain a second domestication solution; diluting with water for the third time to 60% of the original concentration as culture medium, inoculating 10% of the second acclimation solution, and acclimating to obtain a third acclimation solution; adding water for the fourth time to dilute to 80% of the original concentration to be used as a culture medium, inoculating 10% of the third acclimation solution for acclimation, and obtaining kitchen waste microorganism solution;
step three, preparation of fermentation liquor: cooling the starch wastewater to room temperature after high-temperature sterilization, inoculating a proper amount of the kitchen waste microorganism liquid, and fermenting for 40-50h at the temperature of 28-35 ℃ at the temperature of 100-200r/min to obtain fermentation liquid;
step four, extracting the biological flocculant: centrifuging the fermentation liquor to obtain supernatant and precipitate, detecting the pH value of the precipitate, adjusting the pH value to 6-10 with a proper amount of sodium hydroxide solution, and hydrolyzing to obtain hydrolysate; performing ultrasonic crushing and centrifugation on the hydrolysate to obtain secondary supernatant and precipitate, combining the primary supernatant and the secondary supernatant, adding a carbon source, auxiliary materials and a biological activating enzyme, then concentrating to 10-15% of the original volume by adopting an evaporator at the temperature of 30-45 ℃, and performing vacuum drying to obtain biological flocculant powder; and dissolving the biological flocculant powder in water to prepare a solution, thereby obtaining the biological flocculant.
2. The method for preparing a bioflocculant according to claim 1, wherein: in the first step, the rotating speed of a shaking table for domestication culture is 100-200r/min, the temperature for domestication culture is 28-35 ℃, and the domestication time is 48-72 h.
3. The method for preparing a bioflocculant according to claim 1 or 2, wherein: in the third step, the volume of the inoculated kitchen garbage microorganism liquid is 7-10% of the volume of the starch wastewater.
4. The method for preparing a bioflocculant according to claim 1, wherein: in the fourth step, the fermentation liquor is centrifuged at 3000r/min for 10 min.
5. The method for preparing a bioflocculant according to claim 1 or 4, wherein: in the fourth step, the mass concentration of the sodium hydroxide solution is 0.3 mol/L; the hydrolysis time is 40-60 minutes; the drying temperature of the vacuum drying is 100-.
6. The method for preparing a bioflocculant according to claim 1 or 4, wherein: in the fourth step, the mass ratio of the supernatant to the carbon source to the biological activating enzyme is 6:2: 2.
7. The method for preparing a bioflocculant according to claim 1 or 4, wherein: in the fourth step, the carbon source is glucose or sodium acetate; the biological activating enzyme is catalase or amylase; the adjuvants are one or more of Gypsum Fibrosum, diatomaceous earth, and Kaolin.
8. The apparatus for processing a biological flocculant feed material according to any one of claims 1 to 7, comprising a mobile base, characterized in that: the upper end of the movable base is fixed with a processing box, the top end of the processing box is provided with an input pipe for inputting domestic kitchen garbage, an inner cavity of the processing box is provided with a cone hopper-shaped partition plate, a crushing chamber is arranged above the cone hopper-shaped partition plate, double crushing rollers are arranged in the crushing chamber, the center of the cone hopper-shaped partition plate is communicated with the upper side of a sieving barrel through a guide pipe, the sieving barrel is designed in an inclined manner, the high end of the sieving barrel is positioned outside the processing box, the low end of the sieving barrel is positioned inside the processing box, a spiral conveying shaft for sieving is arranged in the sieving barrel, the top end of the spiral conveying shaft is fixedly connected with an output shaft of a sieving motor through a coupling, the sieving motor is fixed on the outer wall of the processing box through a bracket, the lower side of the sieving barrel, which is positioned at the high end, is communicated with an inorganic matter storage barrel through a discharge pipe, and the lower side of the sieving barrel, which is positioned at, the oil discharge pipe of the three-phase centrifuge is communicated with the oil storage cylinder, and the liquid discharge pipe of the three-phase centrifuge is communicated with the collection cylinder.
9. The apparatus for processing a raw material of a bioflocculant according to claim 8, wherein: the spiral conveying shaft is composed of a hollow shaft and a spiral blade fixed on the outer side of the hollow shaft, and at least one of the hollow shaft and the spiral blade is uniformly provided with a filtering hole.
CN202010269382.3A 2020-04-08 2020-04-08 Preparation method of biological flocculant and raw material treatment device Pending CN111533266A (en)

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CN112171943A (en) * 2020-09-30 2021-01-05 安徽中鑫宏伟科技有限公司 Granulator for processing nylon heat insulation strip master batches and using method thereof

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Application publication date: 20200814