CN111518856A - Production process of high-concentration low-molecular-weight liquid chitosan oligosaccharide - Google Patents
Production process of high-concentration low-molecular-weight liquid chitosan oligosaccharide Download PDFInfo
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- CN111518856A CN111518856A CN202010378192.5A CN202010378192A CN111518856A CN 111518856 A CN111518856 A CN 111518856A CN 202010378192 A CN202010378192 A CN 202010378192A CN 111518856 A CN111518856 A CN 111518856A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
Abstract
The invention discloses a production process of high-concentration low-molecular-weight liquid chitosan oligosaccharide, belongs to the technical field of bioengineering, and solves the problems of difficult production of high-concentration liquid chitosan oligosaccharide, difficult absorption of high-molecular products and poor mixing property. The technical key points are as follows: adding acid to swell the high-concentration chitosan, adding ventilation in a reaction kettle, assisting in stirring uniformly, stopping ventilation, stopping stirring, standing for 2-5h, then adding excessive specific high-enzyme activity chitosanase to perform enzymolysis reaction, simultaneously performing ventilation, starting stirring when the viscosity is reduced to 80-100mpa.s, stopping ventilation, and stirring until the reaction is finished. The content of the liquid chitosan oligosaccharide produced by the process can reach 20-25%, which is far higher than the normal production concentration of chitosan oligosaccharide products in the existing market, the molecular weight can be controlled between 600-1500Da, and the induced resistance effect can be better played after the chitosan oligosaccharide is absorbed and utilized by plants. The invention can greatly reduce the production energy consumption, save the production cost, simultaneously reduce the transportation cost and the like, and better improve the production efficiency.
Description
Technical Field
The invention relates to the technical field of bioengineering, in particular to a production process of high-concentration low-molecular-weight liquid chitosan oligosaccharide.
Background
At present, the products of the chitosan oligosaccharide are widely applied to various industries such as agriculture, medicine, health care and the like. At present, the production of chitosan oligosaccharide is mainly prepared by a biological enzyme method, but is limited by the viscosity of raw material chitosan, so that the content of a chitosan oligosaccharide liquid product is always kept at a relatively low level. It is reported that the highest content of liquid chitosan oligosaccharide production is about 15%, which increases the cost in transportation and subsequent spray concentration.
As for the molecular weight of chitosan oligosaccharide, the number average molecular weight of most products is controlled between 1500-4000Da at present, and the high molecular weight (the molecular weight is more than or equal to 5000Da) is relatively high. There are two major problems with relatively high molecular weight chitosan oligosaccharide products: firstly, the plant is not easy to absorb and utilize; secondly, the mixing property with other preparations is poor. It is necessary to control the low molecular weight products.
The production of the high-concentration low-molecular-weight liquid chitosan oligosaccharide can reduce the production and transportation cost, save the energy consumption, labor and the like of later spray drying, save the product cost to a greater extent, and simultaneously, the low-molecular-weight product not only is more beneficial to the absorption and utilization of plants, but also can be better compounded with other preparation products for use, thereby enhancing the application effect. Therefore, it is desirable to provide a process for producing high-content, low-molecular-weight chitosan oligosaccharides.
Disclosure of Invention
Aiming at the problems, the invention provides a production process of high-concentration low-molecular-weight liquid chitosan oligosaccharide, which is mainly characterized in that the process is improved on the basis of the original production of chitosan oligosaccharide by biological enzymolysis, the content of the finally obtained liquid chitosan oligosaccharide product is 20-25%, and the molecular weight of the product is 600-1500 Da.
The specific process technology comprises the following steps: adding acid to swell the high-concentration chitosan, adding ventilation in a reaction kettle, assisting in stirring uniformly, stopping ventilation, stopping stirring, standing for 2-5h, then adding excessive specific high-enzyme activity chitosanase to perform enzymolysis reaction, simultaneously performing ventilation, starting stirring when the viscosity is reduced to 80-100mpa.s, stopping ventilation, and stirring until the reaction is finished.
Wherein the chitosan is flake or powdery solid, and is added into water and uniformly stirred, and the temperature is controlled at 35-45 ℃; the acid used in the swelling process is one or the mixture of any more of formic acid, acetic acid, lactic acid, hydrochloric acid, nitric acid and citric acid; the air quantity for increasing ventilation in the reaction kettle is controlled to be more than or equal to 0.5 vvm; the enzyme activity of the chitosanase used in the process is more than or equal to 2000U, the addition amount is 2-5 times of the normal dosage, the reaction temperature is 35-45 ℃, the reaction time is 2-6h, and the ventilation volume is more than or equal to 1 vvm; the end of the reaction was judged as a decrease in the viscosity of the reaction solution to 3.8-4.0 mpa.s.
The content of the liquid chitosan oligosaccharide produced by the process can reach 20-25%, which is far higher than the normal production concentration of chitosan oligosaccharide products in the existing market, the molecular weight can be controlled between 600-1500Da, and the induced resistance effect can be better played after the chitosan oligosaccharide is absorbed and utilized by plants. The invention can greatly reduce the production energy consumption, save the production cost, simultaneously reduce the transportation cost and the like, and better improve the production efficiency.
Detailed Description
The present invention will be described in further detail with reference to the following examples, but it should not be construed that the scope of the above subject matter is limited to the following examples. All the technologies realized based on the above contents of the present invention belong to the scope of the present invention.
Example 1
A process for preparing the liquid chitooligose with high concentration and low molecular weight features that its content is 20.4% and its molecular weight is 1485 Da.
The preparation method comprises the following specific steps:
adding the flaky chitosan into water, wherein the adding amount is 21%, uniformly stirring, heating to 35 ℃, adding 4% acetic acid for swelling, starting ventilation, the air volume is 0.5vvm, stopping air and stirring after fully and uniformly mixing, standing for 2 hours for swelling, adding 2 times of normal amount of specific chitosanase for enzymolysis reaction after swelling is finished, simultaneously starting ventilation, the air volume is 1vvm, starting stirring when the viscosity is reduced to 80mpa.s, stopping ventilation, reacting for 2 hours, and determining the viscosity of the reaction solution to be 3.98 mpa.s. The chitosan oligosaccharide has a concentration of 20.4% and a molecular weight of 1485 Da.
Example 2
A process for preparing the liquid chitooligose with high concentration and low molecular weight features that its content is 25.1% and its molecular weight is 638 Da.
The preparation method comprises the following specific steps:
adding powdery chitosan into water, adding 26% of chitosan, stirring uniformly, heating to 45 ℃, adding 6.5% of lactic acid for swelling, starting ventilation, enabling the air volume to be 3vvm, stopping air and stirring after fully mixing uniformly, standing for 5 hours for swelling, adding 5 times of normal amount of specific chitosanase for enzymolysis reaction after swelling is finished, simultaneously starting ventilation, enabling the air volume to be 3vvm, starting stirring when the viscosity is reduced to 100mpa.s, stopping ventilation, enabling the reaction time to be 6 hours, and determining the viscosity of the reaction solution to be 3.82 mpa.s. The chitosan oligosaccharide obtained was determined to have a concentration of 25.1% and a molecular weight of 638 Da.
Example 3
A process for preparing high-concentration low-molecular liquid chitooligose features that its content is 23.4% and its molecular weight is 984 Da.
The preparation method comprises the following specific steps:
adding powdery chitosan into water, adding 24% of chitosan, stirring uniformly, heating to 40 ℃, adding 4% of hydrochloric acid for swelling, starting ventilation, enabling the air volume to be 2vvm, fully mixing uniformly, stopping air and stirring, standing for 4 hours for swelling, adding 3 times of the normal amount of specific chitosanase after swelling is completed for enzymolysis reaction, simultaneously starting ventilation, enabling the air volume to be 2vvm, starting stirring when the viscosity is reduced to 90mpa.s, stopping ventilation, enabling the reaction time to be 5 hours, and determining the viscosity of the reaction solution to be 3.88 mpa.s. The chitosan oligosaccharide obtained by determination has a concentration of 23.4% and a molecular weight of 984 Da.
Example 4
A process for preparing the liquid chitooligose with high concentration and low molecular weight features that its content is 21.3% and its molecular weight is 1247 Da.
The preparation method comprises the following specific steps:
adding flake chitosan into water, adding 22% of chitosan, stirring uniformly, heating to 42 ℃, adding a mixed solution of 4.5% acetic acid and nitric acid for swelling, starting ventilation, wherein the air volume is 1.5vvm, stopping air and stirring after fully and uniformly mixing, standing for 3 hours for swelling, adding 4 times of normal dosage of specific chitosanase after swelling for enzymolysis, starting ventilation, wherein the air volume is 2.5vvm, starting stirring when the viscosity is reduced to 85mpa.s, stopping ventilation, reacting for 4 hours, and determining the viscosity of the reaction solution to be 3.92 mpa.s. The chitosan oligosaccharide obtained was determined to have a concentration of 21.3% and a molecular weight of 1247 Da.
While the preferred embodiments of the present invention have been described in detail, the present invention is not limited to the above embodiments, and various changes can be made without departing from the spirit of the present invention within the knowledge of those skilled in the art.
Claims (6)
1. A production process of high-concentration low-molecular-weight liquid chitosan oligosaccharide is characterized by comprising the following steps: adding acid to swell the high-concentration chitosan, adding ventilation in a reaction kettle, uniformly stirring in an auxiliary way, stopping wind, stopping stirring, standing for 2-5h, adding excessive specific high-enzyme activity chitosanase after swelling is finished, carrying out enzymolysis reaction, simultaneously ventilating, starting stirring when the viscosity is reduced to 80-100mpa.s, stopping ventilation, and stirring until the reaction is finished.
2. The process for producing high-concentration, low-molecular-weight liquid chitosan oligosaccharide according to claim 1, wherein: the highest adding concentration of the raw material chitosan can reach 25 percent, the chitosan is flaky or powdery solid, the chitosan is added into water and then stirred uniformly, and the temperature is controlled at 35-45 ℃.
3. The process for producing high-concentration, low-molecular-weight liquid chitosan oligosaccharide according to claim 1, wherein: the acid used in the swelling process is one or the mixture of any of formic acid, acetic acid, lactic acid, hydrochloric acid, nitric acid and citric acid.
4. The process for producing high-concentration, low-molecular-weight liquid chitosan oligosaccharide according to claim 1, wherein: the reaction kettle is ventilated, and the ventilation quantity is controlled to be more than or equal to 0.5 vvm.
5. The process for producing high-concentration, low-molecular-weight liquid chitosan oligosaccharide according to claim 1, wherein: the enzyme activity of the chitosanase is more than or equal to 2000U, the addition amount of the chitosanase is 2-5 times of the normal dosage, the reaction temperature is 35-45 ℃, the reaction time is 2-6h, and the ventilation volume is more than or equal to 1 vvm.
6. The process for producing high-concentration, low-molecular-weight liquid chitosan oligosaccharide according to claim 1, wherein: the end of the reaction is marked by the viscosity of the reaction solution decreasing to 3.8-4.0mpa.s, and the molecular weight can be controlled between 600 and 1500 Da.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112608958A (en) * | 2020-12-17 | 2021-04-06 | 黄河三角洲京博化工研究院有限公司 | Chitosan oligosaccharide preparation method and weight-losing tablets |
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| CN101240305A (en) * | 2007-09-06 | 2008-08-13 | 武汉东方天琪生物工程有限公司 | Method for producing Chitosan oligosaccharide by using enzyme method to degrade chitosan |
| CN101381752A (en) * | 2008-10-17 | 2009-03-11 | 扬州日兴生物科技股份有限公司 | Technique for preparing low chitose and chitosan oligosaccharide |
| CN104846037A (en) * | 2015-05-29 | 2015-08-19 | 领先生物农业股份有限公司 | High-concentration chitosan oligosaccharide and production method thereof |
| CN105154496A (en) * | 2015-08-21 | 2015-12-16 | 黄河三角洲京博化工研究院有限公司 | Enzymatic preparation method of water soluble chitosan with specific molecular weight |
| CN110066838A (en) * | 2019-06-12 | 2019-07-30 | 山东昌瑞生物科技有限公司 | A kind of production method of high amount of liquid chitosan oligosaccharide |
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2020
- 2020-05-07 CN CN202010378192.5A patent/CN111518856A/en active Pending
Patent Citations (5)
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| CN101240305A (en) * | 2007-09-06 | 2008-08-13 | 武汉东方天琪生物工程有限公司 | Method for producing Chitosan oligosaccharide by using enzyme method to degrade chitosan |
| CN101381752A (en) * | 2008-10-17 | 2009-03-11 | 扬州日兴生物科技股份有限公司 | Technique for preparing low chitose and chitosan oligosaccharide |
| CN104846037A (en) * | 2015-05-29 | 2015-08-19 | 领先生物农业股份有限公司 | High-concentration chitosan oligosaccharide and production method thereof |
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| CN110066838A (en) * | 2019-06-12 | 2019-07-30 | 山东昌瑞生物科技有限公司 | A kind of production method of high amount of liquid chitosan oligosaccharide |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112608958A (en) * | 2020-12-17 | 2021-04-06 | 黄河三角洲京博化工研究院有限公司 | Chitosan oligosaccharide preparation method and weight-losing tablets |
| CN112608958B (en) * | 2020-12-17 | 2022-12-02 | 黄河三角洲京博化工研究院有限公司 | Chitosan oligosaccharide preparation method and weight-losing tablets |
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