CN111513194A - High-temperature-resistant cyclodextrin inclusion enzyme preparation and preparation method thereof - Google Patents
High-temperature-resistant cyclodextrin inclusion enzyme preparation and preparation method thereof Download PDFInfo
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- CN111513194A CN111513194A CN202010293341.8A CN202010293341A CN111513194A CN 111513194 A CN111513194 A CN 111513194A CN 202010293341 A CN202010293341 A CN 202010293341A CN 111513194 A CN111513194 A CN 111513194A
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- 102000004190 Enzymes Human genes 0.000 title claims abstract description 47
- 108090000790 Enzymes Proteins 0.000 title claims abstract description 47
- 238000002360 preparation method Methods 0.000 title claims abstract description 46
- 229920000858 Cyclodextrin Polymers 0.000 title claims abstract description 23
- HFHDHCJBZVLPGP-UHFFFAOYSA-N schardinger α-dextrin Chemical compound O1C(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(O)C2O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC2C(O)C(O)C1OC2CO HFHDHCJBZVLPGP-UHFFFAOYSA-N 0.000 title claims abstract description 14
- 229940088598 enzyme Drugs 0.000 claims abstract description 46
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 31
- 244000309464 bull Species 0.000 claims description 18
- 238000007789 sealing Methods 0.000 claims description 16
- 238000003756 stirring Methods 0.000 claims description 15
- 239000000463 material Substances 0.000 claims description 14
- 101710130006 Beta-glucanase Proteins 0.000 claims description 13
- 238000002156 mixing Methods 0.000 claims description 12
- 239000001116 FEMA 4028 Substances 0.000 claims description 9
- WHGYBXFWUBPSRW-FOUAGVGXSA-N beta-cyclodextrin Chemical compound OC[C@H]([C@H]([C@@H]([C@H]1O)O)O[C@H]2O[C@@H]([C@@H](O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O3)[C@H](O)[C@H]2O)CO)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H]3O[C@@H]1CO WHGYBXFWUBPSRW-FOUAGVGXSA-N 0.000 claims description 9
- 235000011175 beta-cyclodextrine Nutrition 0.000 claims description 9
- 229960004853 betadex Drugs 0.000 claims description 9
- 239000001267 polyvinylpyrrolidone Substances 0.000 claims description 9
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 claims description 9
- 229920000036 polyvinylpyrrolidone Polymers 0.000 claims description 9
- 239000008367 deionised water Substances 0.000 claims description 8
- 229910021641 deionized water Inorganic materials 0.000 claims description 8
- 238000009736 wetting Methods 0.000 claims description 8
- 108010011619 6-Phytase Proteins 0.000 claims description 6
- 239000004382 Amylase Substances 0.000 claims description 6
- 102000013142 Amylases Human genes 0.000 claims description 6
- 108010065511 Amylases Proteins 0.000 claims description 6
- 108010059892 Cellulase Proteins 0.000 claims description 6
- 101710121765 Endo-1,4-beta-xylanase Proteins 0.000 claims description 6
- 239000004366 Glucose oxidase Substances 0.000 claims description 6
- 108010015776 Glucose oxidase Proteins 0.000 claims description 6
- 108091005804 Peptidases Proteins 0.000 claims description 6
- 108010059820 Polygalacturonase Proteins 0.000 claims description 6
- 239000004365 Protease Substances 0.000 claims description 6
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 6
- 235000019418 amylase Nutrition 0.000 claims description 6
- 229940106157 cellulase Drugs 0.000 claims description 6
- 108010093305 exopolygalacturonase Proteins 0.000 claims description 6
- 229940116332 glucose oxidase Drugs 0.000 claims description 6
- 235000019420 glucose oxidase Nutrition 0.000 claims description 6
- 229940085127 phytase Drugs 0.000 claims description 6
- 235000019419 proteases Nutrition 0.000 claims description 6
- 238000007599 discharging Methods 0.000 claims description 5
- 238000010298 pulverizing process Methods 0.000 claims description 5
- 239000000443 aerosol Substances 0.000 claims description 4
- 238000001816 cooling Methods 0.000 claims description 4
- 238000005469 granulation Methods 0.000 claims description 4
- 230000003179 granulation Effects 0.000 claims description 4
- 239000002994 raw material Substances 0.000 claims description 4
- 239000007921 spray Substances 0.000 claims description 2
- 230000007704 transition Effects 0.000 claims description 2
- 108010055059 beta-Mannosidase Proteins 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 18
- 238000000034 method Methods 0.000 abstract description 8
- 229940079919 digestives enzyme preparation Drugs 0.000 abstract description 7
- 238000012545 processing Methods 0.000 abstract description 5
- 244000144972 livestock Species 0.000 abstract description 2
- 244000144977 poultry Species 0.000 abstract description 2
- 239000003674 animal food additive Substances 0.000 abstract 1
- 238000003860 storage Methods 0.000 description 13
- 238000004378 air conditioning Methods 0.000 description 10
- 239000000203 mixture Substances 0.000 description 9
- -1 beta-mannase Proteins 0.000 description 5
- 230000000052 comparative effect Effects 0.000 description 5
- 238000005303 weighing Methods 0.000 description 3
- 230000004308 accommodation Effects 0.000 description 2
- 238000010030 laminating Methods 0.000 description 2
- 238000005507 spraying Methods 0.000 description 2
- 238000003466 welding Methods 0.000 description 2
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000000498 cooling water Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 235000019621 digestibility Nutrition 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/116—Heterocyclic compounds
- A23K20/132—Heterocyclic compounds containing only one nitrogen as hetero atom
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/10—Shaping or working-up of animal feeding-stuffs by agglomeration; by granulation, e.g. making powders
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/30—Shaping or working-up of animal feeding-stuffs by encapsulating; by coating
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23N—MACHINES OR APPARATUS FOR TREATING HARVESTED FRUIT, VEGETABLES OR FLOWER BULBS IN BULK, NOT OTHERWISE PROVIDED FOR; PEELING VEGETABLES OR FRUIT IN BULK; APPARATUS FOR PREPARING ANIMAL FEEDING- STUFFS
- A23N17/00—Apparatus specially adapted for preparing animal feeding-stuffs
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
- B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
- B02C13/00—Disintegrating by mills having rotary beater elements ; Hammer mills
- B02C13/14—Disintegrating by mills having rotary beater elements ; Hammer mills with vertical rotor shaft, e.g. combined with sifting devices
- B02C13/18—Disintegrating by mills having rotary beater elements ; Hammer mills with vertical rotor shaft, e.g. combined with sifting devices with beaters rigidly connected to the rotor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
- B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
- B02C23/00—Auxiliary methods or auxiliary devices or accessories specially adapted for crushing or disintegrating not provided for in preceding groups or not specially adapted to apparatus covered by a single preceding group
- B02C23/18—Adding fluid, other than for crushing or disintegrating by fluid energy
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
The invention relates to the technical field of livestock and poultry feed additive preparations, in particular to a high-temperature-resistant cyclodextrin inclusion enzyme preparation and a preparation method thereof. The invention solves the problems that most enzyme preparations are not high-temperature resistant and can not keep activity in the feed processing process, and is worthy of popularization.
Description
Technical Field
The invention relates to the technical field of enzyme preparations, in particular to a high-temperature-resistant cyclodextrin inclusion enzyme preparation and a preparation method thereof.
Background
The development concept of green and environment-friendly in the 21 st century is deep, and the enzyme preparation which is taken as an antibiotic substitute and applied to the feed at present is mainly characterized by comprising the following components: the enzyme preparations such as amylase, protease, glucose oxidase, pectinase, xylanase, beta-mannase, cellulase, beta-glucanase, phytase and related complex enzyme and the like have important effects on improving the digestibility of livestock and poultry nutrient substances, but the granular feed needs to be subjected to high temperature of 70-85 ℃ in the processing process, most enzyme preparations are not high-temperature resistant and cannot keep activity in the processing process of the feed, and therefore the utilization rate of the enzyme preparations needs to be improved through the treatment of a preparation process.
Disclosure of Invention
The invention aims to solve the defects that most enzyme preparations in the prior art cannot resist high temperature and cannot keep activity in the feed processing process, and provides a high-temperature-resistant cyclodextrin inclusion enzyme preparation and a preparation method thereof.
In order to achieve the purpose, the invention adopts the following technical scheme:
designing a high temperature resistant cyclodextrin inclusion enzyme preparation and a preparation method thereof, wherein the preparation steps are as follows;
(1) wetting enzyme preparation raw materials by using deionized water, wherein the addition amount of the deionized water is 10-15% of the mass of the enzyme, and wetting by using aerosol spray;
(2) and (3) putting the moistened enzyme preparation and the auxiliary materials into a mixing device for mixing, wherein the mixing ratio is that the moistened enzyme preparation is beta-cyclodextrin: uniformly stirring and mixing polyvinylpyrrolidone in a ratio of 2:5:3, discharging, and collecting the discharged material;
(3) adding the collected discharged materials into crushing equipment, wherein the feeding amount is 2/3 which is the maximum amount of the crushing equipment, crushing is carried out for 30-40min, and the crushing equipment is additionally provided with a water cooling system;
(4) and (4) performing swing granulation on the crushed materials, wherein the granularity is 20-40 meshes.
Preferably, the enzyme preparation is one or more of amylase, protease, glucose oxidase, pectinase, xylanase, beta-mannase, cellulase, beta-glucanase and phytase.
Preferably, the crushing equipment comprises a containing shell, a rotating rod is inserted into the top end of the containing shell, one end of the rotating rod is inserted into the inner side of the containing shell, a connecting rod is fixedly connected to the side surface of the rotating rod and is positioned on the inner side of the containing shell, a stirring rod is welded on the side surface of the connecting rod, one end of the connecting rod, far away from the rotating rod, is fixedly connected with a crushing block, a plurality of through holes are formed in the bottom of the side surface of the containing shell, opposite to the crushing block, of the containing shell, a driven gear is fixedly connected to one end of the containing shell, a motor is fixedly connected to an output shaft of the motor, a driving gear is meshed with the driven gear, air ducts are formed in the rotating rod and the connecting rod, the air ducts in the rotating rod are communicated with the air ducts in the connecting rod, an air inlet, one end of the stirring rod is provided with an air outlet, the air outlet is communicated with the air channel in the connecting rod, and the top end of the accommodating shell is provided with a feeding hole.
Preferably, hold casing side bottom fixedly connected with storage housing, storage housing side bottom is provided with the discharge opening, storage housing top threaded connection has a plurality of control bolts, placed sealing ring, spring in the storage housing, the relative through-hole setting of sealing ring, the threaded one end of control bolt stretches into the storage housing inboard, the spring housing is in the control bolt outside, the both ends of spring respectively with sealing ring, storage housing fixed connection, be transition fit between sealing ring and the storage housing.
Preferably, the top end of the accommodating shell is communicated with an air outlet pipe, and the side surface of the stirring rod is provided with a plurality of through holes.
The high-temperature-resistant cyclodextrin inclusion enzyme preparation and the preparation method thereof have the beneficial effects that:
the enzyme preparation, the beta-cyclodextrin and the polyvinylpyrrolidone are mixed according to a certain proportion, and then the mixed mixture is crushed and granulated, so that the beta-cyclodextrin and the polyvinylpyrrolidone are used as carriers of the enzyme preparation, and the high temperature resistance and the bioavailability of the enzyme preparation are improved.
Drawings
FIG. 1 is a schematic diagram of the structure of a high temperature resistant cyclodextrin inclusion enzyme preparation and a crushing device in the preparation method thereof according to the present invention;
FIG. 2 is a cross-sectional view at A-A of FIG. 1;
fig. 3 is a partial enlarged view of fig. 1 at B.
In the figure: the air conditioner comprises an accommodating shell 1, an air outlet pipe 2, a motor 3, a driving gear 4, a driven gear 5, a rotating rod 6, a control bolt 7, a seal 8, a spring 9, an accommodating shell 10, an air duct 11, a stirring rod 12, a through hole 13, a connecting rod 14, a crushing block 15, a through hole 16, an air inlet pipe 17, an water inlet pipe 18, a water outlet pipe 19 and a spiral water channel 20.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
Example one
Referring to fig. 1-3, a high temperature resistant cyclodextrin inclusion enzyme preparation and a method for preparing the same, the preparation steps are as follows;
(1) weighing 100 kg of beta-mannase and 15 kg of deionized water, wetting the beta-mannase by using wetting deionized water by adopting an aerosol spraying method,
(2) mixing the wetted beta-mannase, beta-cyclodextrin and polyvinylpyrrolidone according to the mass ratio of 2:5:3, uniformly stirring and discharging, collecting discharged materials,
(3) the collected output was fed to the comminution apparatus at 2/3, the maximum input for the comminution apparatus. Pulverizing for 30-40min, adding water cooling system to the pulverizing equipment to avoid unnecessary loss of enzyme activity due to high temperature,
(4) and (4) performing swing granulation on the crushed materials, wherein the granularity is 20-40 meshes.
The enzyme preparation is one or more of amylase, protease, glucose oxidase, pectinase, xylanase, beta-mannase, cellulase, beta-glucanase and phytase.
Crushing apparatus is including holding casing 1, and it is hollow structure to hold casing 1, holds 1 top of casing and has inserted bull stick 6, and 6 one end of bull stick is inserted and is held 1 inboardly of casing, 6 side fixedly connected with connecting rods 14 of bull stick, and connecting rod 14 are located and hold 1 inboards of casing, and the welding of connecting rod 14 side has puddler 12, stirs putting into the mixture that holds in the casing 1 through connecting rod 14 and puddler 12.
One end fixedly connected with that bull stick 6 was kept away from to connecting rod 14 smashes piece 15, smashes piece 15 and keeps away from the part in connecting rod 14 one side and hold 1 inboard laminating of casing, holds that 15 departments of smashing the piece relatively in 1 side bottom of casing open and have a plurality of through-holes 16, beta-cyclodextrin: the part after polyvinylpyrrolidone is smashed passes through 16 plays accommodation casing 1 of through-hole, 6 one end fixedly connected with driven gear 5 of bull stick, it is fixed with motor 3 to hold 1 top of casing, motor 3 external power supply, motor 3 output shaft fixedly connected with driving gear 4, driving gear 4 and the meshing of driven gear 5 rotate through motor 3 drive driven gear 5 and rotate, thereby make bull stick 6 hold casing 1 relatively and rotate, bull stick 6 and hold and be clearance fit between the casing 1.
The utility model provides a rotor 6, be provided with wind channel 11 in the connecting rod 14, and wind channel intercommunication in wind channel and the connecting rod 14 in the rotor 6, 6 one end fixedly connected with air-supply line 17 of rotor are kept away from to driven gear 5, air-supply line 17 is located driven gear 5 axis department, the external air conditioning feeding device of air-supply line 17, air conditioning feeding device supplies air conditioning to wind channel 11 when rotor 6 rotates, air- supply line 17 and 6 interior wind channel 11 intercommunication of rotor, 12 one end openings of puddler have the exhaust vent, the air conditioning that air conditioning feeding device produced gets into wind channel 11 through air-supply line 17, then get into through the exhaust vent and hold casing 1 in, exhaust vent and 14 interior wind channel intercommunication of connecting rod, it has the feed port to hold casing 1 top to open, put in the mixture to holding casing 1 through the feed.
The bottom of the side surface of the containing shell 1 is fixedly connected with a containing shell 10, the containing shell 10 is used for storing the crushed mixture, the bottom of the side surface of the containing shell 10 is provided with a discharge hole, the top end of the containing shell 10 is in threaded connection with a plurality of control bolts 7, the control bolts 7 are in threaded connection with the containing shell 10, a sealing ring 8 and a spring 9 are placed in the containing shell 10, the sealing ring 8 is arranged relative to a through hole 16, one threaded end of each control bolt 7 extends into the inner side of the containing shell 10, the spring 9 is sleeved on the outer side of the control bolt 7, two ends of the spring 9 are respectively and fixedly connected with the sealing ring 8 and the containing shell 10, the sealing, the control bolt 7 can control the positions of the seal ring 8 and the storage case 10 by rotating the control bolt 7 relative to the storage case 10 so that the position of the seal ring 8 relative to the storage case 10 is changed.
The top end of the accommodating shell body 1 is communicated with an air outlet pipe 2, after the feeding hole is sealed through the air outlet pipe 2, cold air passes through the air outlet pipe 2 to be accommodated in the accommodating shell body 1, the side face of the stirring rod 12 is provided with a plurality of through holes 13, the air channel blockage is avoided through the plurality of through holes 13, a plurality of air outlet holes can be further formed in the side face of the connecting rod 14, and after the through holes 13 are prevented from being blocked, the cold air in the air channel 11 cannot enter the mixture in the accommodating shell body 1.
The water cooling system comprises a water inlet pipe 18, a water outlet pipe 19 and a spiral water channel 20, wherein the spiral water channel 20 is arranged in the accommodating shell 1, the water inlet pipe 18 is fixedly connected to the side face of the accommodating shell 1, the water inlet pipe 18 is communicated with the spiral water channel 20, the water outlet pipe 19 is communicated with the spiral water channel 20, and cooling water enters the spiral water channel 20 through the water inlet pipe 18 and then flows out of the water outlet pipe 19.
Example two
Referring to fig. 1-3, a high temperature resistant cyclodextrin inclusion enzyme preparation and a method for preparing the same, the preparation steps are as follows;
(1) weighing amylase, protease, glucose oxidase, pectinase, xylanase, beta-mannase, cellulase, beta-glucanase, phytase and 10 kg, and mixing uniformly to obtain a compound enzyme; weighing 10 kg of deionized water, wetting the complex enzyme by using wetting deionized water by adopting an aerosol spraying method,
(2) mixing the wetted beta-mannase, beta-cyclodextrin and polyvinylpyrrolidone according to the mass ratio of 2:5:3, uniformly stirring and discharging, collecting discharged materials,
(3) mixing the wetted complex enzyme with beta-cyclodextrin and polyvinylpyrrolidone according to the mass ratio of 2:5:3, uniformly stirring and discharging, collecting discharged materials,
(4) and (4) performing swing granulation on the crushed materials, wherein the granularity is 20-40 meshes.
The enzyme preparation is one or more of amylase, protease, glucose oxidase, pectinase, xylanase, beta-mannase, cellulase, beta-glucanase and phytase.
Crushing apparatus is including holding casing 1, and it is hollow structure to hold casing 1, holds 1 top of casing and has inserted bull stick 6, and 6 one end of bull stick is inserted and is held 1 inboardly of casing, 6 side fixedly connected with connecting rods 14 of bull stick, and connecting rod 14 are located and hold 1 inboards of casing, and the welding of connecting rod 14 side has puddler 12, stirs putting into the mixture that holds in the casing 1 through connecting rod 14 and puddler 12.
One end fixedly connected with that bull stick 6 was kept away from to connecting rod 14 smashes piece 15, smashes piece 15 and keeps away from the part in connecting rod 14 one side and hold 1 inboard laminating of casing, holds that 15 departments of smashing the piece relatively in 1 side bottom of casing open and have a plurality of through-holes 16, beta-cyclodextrin: the part after polyvinylpyrrolidone is smashed passes through 16 plays accommodation casing 1 of through-hole, 6 one end fixedly connected with driven gear 5 of bull stick, it is fixed with motor 3 to hold 1 top of casing, motor 3 external power supply, motor 3 output shaft fixedly connected with driving gear 4, driving gear 4 and the meshing of driven gear 5 rotate through motor 3 drive driven gear 5 and rotate, thereby make bull stick 6 hold casing 1 relatively and rotate, bull stick 6 and hold and be clearance fit between the casing 1.
The utility model provides a rotor 6, be provided with wind channel 11 in the connecting rod 14, and wind channel intercommunication in wind channel and the connecting rod 14 in the rotor 6, 6 one end fixedly connected with air-supply line 17 of rotor are kept away from to driven gear 5, air-supply line 17 is located driven gear 5 axis department, the external air conditioning feeding device of air-supply line 17, air conditioning feeding device supplies air conditioning to wind channel 11 when rotor 6 rotates, air- supply line 17 and 6 interior wind channel 11 intercommunication of rotor, 12 one end openings of puddler have the exhaust vent, the air conditioning that air conditioning feeding device produced gets into wind channel 11 through air-supply line 17, then get into through the exhaust vent and hold casing 1 in, exhaust vent and 14 interior wind channel intercommunication of connecting rod, it has the feed port to hold casing 1 top to open, put in the mixture to holding casing 1 through the feed.
The bottom of the side surface of the containing shell 1 is fixedly connected with a containing shell 10, the containing shell 10 is used for storing the crushed mixture, the bottom of the side surface of the containing shell 10 is provided with a discharge hole, the top end of the containing shell 10 is in threaded connection with a plurality of control bolts 7, the control bolts 7 are in threaded connection with the containing shell 10, a sealing ring 8 and a spring 9 are placed in the containing shell 10, the sealing ring 8 is arranged relative to a through hole 16, one threaded end of each control bolt 7 extends into the inner side of the containing shell 10, the spring 9 is sleeved on the outer side of the control bolt 7, two ends of the spring 9 are respectively and fixedly connected with the sealing ring 8 and the containing shell 10, the sealing, the control bolt 7 can control the positions of the seal ring 8 and the storage case 10 by rotating the control bolt 7 relative to the storage case 10 so that the position of the seal ring 8 relative to the storage case 10 is changed.
The top end of the accommodating shell body 1 is communicated with an air outlet pipe 2, after the feeding hole is sealed through the air outlet pipe 2, cold air passes through the air outlet pipe 2 to be accommodated in the accommodating shell body 1, the side face of the stirring rod 12 is provided with a plurality of through holes 13, the air channel blockage is avoided through the plurality of through holes 13, a plurality of air outlet holes can be further formed in the side face of the connecting rod 14, and after the through holes 13 are prevented from being blocked, the cold air in the air channel 11 cannot enter the mixture in the accommodating shell body 1.
Comparative experiment:
untreated normal beta-glucanase feedstock
The enzyme preparations of example 1 and the conventional beta-glucanase raw material (comparative example) were sufficiently dissolved in water at a constant temperature of 39 ℃ in an amount of 1000mg/kg, and the beta-glucanase activity was measured in water baths at 75 ℃ for 3 minutes and 5 minutes and at 85 ℃ for 3 minutes and 5 minutes, respectively, and the enzyme activity retention rate was calculated (based on 100% of the initial enzyme activity)
TABLE 1 enzyme Activity at different temperatures
| Group of | Examples1 | Comparative example |
| Initial enzyme activity | 100％ | 100％ |
| Enzyme activity in 75 deg.C water bath for 3 min | 97％ | 12％ |
| Enzyme activity in 75 deg.C water bath for 5 min | 95％ | 2％ |
| Enzyme activity in water bath at 85 deg.C for 3 min | 95％ | 5％ |
| Enzyme activity in water bath at 85 deg.C for 5 min | 90％ | 0％ |
The test result shows that: the enzyme activity of the beta-glucanase preparation in the example 1 is still kept 95 percent after being soaked in water at 75 ℃ for 5 minutes, while the enzyme activity of the common beta-glucanase raw material in the comparative example is only 2 percent after being soaked in water at 75 ℃ for 5 minutes; the beta-glucanase preparation of example 1 still had 90% enzyme activity after 5 minutes in a water bath at 85 ℃, whereas the normal beta-glucanase material of the comparative example had no enzyme activity after 5 minutes in a water bath at 75 ℃. The technical method of the invention can obviously improve the heat resistance of enzyme products, so that the enzyme can resist the high temperature in the feed processing process, and is more suitable for being applied in the feed.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (5)
1. A preparation method of high temperature resistant cyclodextrin inclusion enzyme preparation comprises the following steps;
(1) wetting enzyme preparation raw materials by using deionized water, wherein the addition amount of the deionized water is 10-15% of the mass of the enzyme, and wetting by using aerosol spray;
(2) and (3) putting the moistened enzyme preparation and the auxiliary materials into a mixing device for mixing, wherein the mixing ratio is that the moistened enzyme preparation is beta-cyclodextrin: uniformly stirring and mixing polyvinylpyrrolidone in a ratio of 2:5:3, discharging, and collecting the discharged material;
(3) adding the collected discharged materials into crushing equipment, wherein the feeding amount is 2/3 which is the maximum amount of the crushing equipment, crushing is carried out for 30-40min, and the crushing equipment is additionally provided with a water cooling system;
(4) and (4) performing swing granulation on the crushed materials, wherein the granularity is 20-40 meshes.
2. The preparation of claim 1, wherein the enzyme preparation is one or more of amylase, protease, glucose oxidase, pectinase, xylanase, beta-mannanase, cellulase, beta-glucanase, and phytase.
3. The high temperature resistant cyclodextrin inclusion enzyme preparation and the preparation method thereof according to claim 1, wherein the pulverizing apparatus comprises a housing (1), a rotating rod (6) is inserted into the top end of the housing (1), one end of the rotating rod (6) is inserted into the inside of the housing (1), a connecting rod (14) is fixedly connected to the side of the rotating rod (6), the connecting rod (14) is located inside the housing (1), a stirring rod (12) is welded to the side of the connecting rod (14), one end of the connecting rod (14) far away from the rotating rod (6) is fixedly connected with a pulverizing block (15), a plurality of through holes (16) are formed at the bottom of the side of the housing (1) corresponding to the pulverizing block (15), one end of the rotating rod (6) is fixedly connected with a driven gear (5), and a motor (3) is fixed to the top end of the housing (1), motor (3) output shaft fixedly connected with driving gear (4), driving gear (4) and driven gear (5) meshing, be provided with wind channel (11) in bull stick (6), connecting rod (14), just wind channel in bull stick (6) with wind channel intercommunication in connecting rod (14), bull stick (6) one end fixedly connected with air-supply line (17) are kept away from in driven gear (5), air-supply line (17) with wind channel (11) intercommunication in bull stick (6), puddler (12) one end is opened there is the exhaust vent, the exhaust vent with wind channel intercommunication in connecting rod (14), it has the feed port to open on holding casing (1) top.
4. The preparation of high temperature cyclodextrin inclusion synthase according to claim 3, it is characterized in that the bottom of the side surface of the accommodating shell (1) is fixedly connected with an accommodating shell (10), a discharge hole is arranged at the bottom of the side surface of the containing shell (10), a plurality of control bolts (7) are connected with the top end of the containing shell (10) in a threaded manner, a sealing ring (8) and a spring (9) are arranged in the containing shell (10), the sealing ring (8) is arranged opposite to the through hole (16), one end with threads of the control bolt (7) extends into the inner side of the containing shell (10), the spring (9) is sleeved outside the control bolt (7), two ends of the spring (9) are respectively fixedly connected with the sealing ring (8) and the containing shell (10), the sealing ring (8) is in transition fit with the accommodating shell (1).
5. The preparation method of the high temperature resistant cyclodextrin inclusion synthase according to claim 3, wherein the top end of the containing shell (1) is communicated with an air outlet pipe (2), and the side surface of the stirring rod (12) is provided with a plurality of through holes (13).
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