CN111500501B

CN111500501B - Streptomyces misonii strain and application thereof in preventing and treating wheat root rot and stem basal rot

Info

Publication number: CN111500501B
Application number: CN202010376134.9A
Authority: CN
Inventors: 魏士平; 边佳琪
Original assignee: China University of Geosciences Beijing
Current assignee: China University of Geosciences Beijing
Priority date: 2020-05-07
Filing date: 2020-05-07
Publication date: 2022-02-01
Anticipated expiration: 2040-05-07
Also published as: CN111500501A

Abstract

The invention discloses an actinomycete with antagonistic action on wheat root rot pathogenic bacteria and stem rot pathogenic bacteria and application thereof. The actinomycete is Streptomyces missense Q1C-5CGMCC NO. 18008. The compound preparation has antagonistic action on rhizoctonia solani (Bipolaris sorokiniana), Fusarium graminearum (Fusarium graminearum) and Fusarium pseudograminearum, can effectively inhibit hypha elongation and spore germination of pathogenic fungi, and can be used for preventing and treating wheat root rot caused by the rhizoctonia solani and wheat stalk rot caused by the Fusarium pseudograminearum.

Description

Streptomyces misonii strain and application thereof in preventing and treating wheat root rot and stem basal rot

Technical Field

The invention relates to streptomyces mairei with antagonistic effect on wheat root rot cells, fusarium graminearum and fusarium pseudograminearum and application thereof in preventing and treating wheat root rot and wheat stem rot.

Background

Along with the improvement of living standard of people, people pay more and more attention to a green agriculture development mode, namely, the agriculture development mode which can protect the environment and ensure green and pollution-free agricultural products while promoting the agriculture development. However, in the actual production process of China, due to continuous cropping of crops, certain pathogenic bacteria attack in successive years to form diseased soil; in addition, due to the reduction of organic matters in soil caused by long-term application of fertilizers and pesticides, the microbial ecology of the soil is destroyed, so that soil-borne diseases are aggravated in successive years. Although people obtain certain effect through chemical prevention and control technology, long-term use of chemical preparations also destroys the soil aggregate structure and physicochemical properties, further aggravates the soil ecological environment, and simultaneously causes a series of problems of crop drug resistance accumulation, agricultural product drug residue and the like. The beneficial microorganisms are utilized to improve the biological community in the soil, eliminate or inhibit pathogenic bacteria in the soil, reduce the use of pesticides and restore the soil ecosystem, which is a necessary way for the development of green agriculture in the future.

Biological control measures for controlling plant diseases by using beneficial microorganisms have become mainstream of research and application due to the characteristics of safety, low toxicity, environmental friendliness and the like. Actinomycetes are an important microbial resource, can produce various active compounds, and are used as biocontrol bacteria for preventing and treating agricultural diseases and insect pests. In recent years, some scholars have been searching for new actinomycete resources due to the difficulty in finding more novel drugs and bioactive substances from terrestrial actinomycetes. The particularity of marine environment determines that the structure and bioactive substances of secondary metabolites generated by marine actinomycetes are different from those of terrestrial actinomycetes, so that the marine actinomycetes has more diversity. The marine actinomycete as one new field can produce various compounds with novel structure, has obvious research and development prospects on bacteriostatic activity and disease resistance mechanism, and can produce various secondary metabolites as bioactive compounds, especially streptomyces. At present, various bioactive natural products such as alkaloids, polyketones, peptides, macrolides and the like are separated from marine actinomycetes, and can resist bacteria, fungi and tumors or have cytotoxic activity and the like. The invention takes the actinomycetes in the sea as a research object, and prepares the actinomycetes into a microbial preparation for green agricultural production through the experiments of separation and screening of the actinomycetes, prevention and treatment of diseases and the like, thereby having wide development prospect.

Wheat is a gramineous crop widely planted around the world and is also one of the main food crops in China. The method is mainly centralized in winter wheat areas at the middle and lower reaches of Yangtze river, northeast spring wheat area, northwest spring wheat area, southwest wheat area and Qinghai-Tibet plateau winter spring wheat area, wherein the wheat yield of the three provinces of Henan, Shandong and Hebei accounts for more than 50% of the total wheat yield of the whole country every year. Plant diseases are the most important reason for limiting the yield increase of wheat, and the diseases harmful to wheat in China mainly comprise: stripe rust, leaf rust, stem rust, stinking smut, loose smut, yellow dwarf, red dwarf, take-all, leaf spot and the like of wheat. The wheat root rot and stem base rot occur in the northeast, northwest and inner Mongolia wheat areas of China, which can cause large area yield reduction of wheat. But because the disease belongs to soil-borne diseases, the chemical prevention and control method is difficult to work; moreover, the use of chemical agents also leads to increased resistance of pathogenic bacteria and thus to environmental pollution. Therefore, the application of green pollution-free biological control to control wheat soil-borne diseases is the direction of future development.

Disclosure of Invention

The invention aims to provide an actinomycete with antagonistic action on wheat root rot pathogenic bacteria and wheat stem base rot pathogenic bacteria and application thereof in preventing and treating wheat root rot and wheat stem base rot.

The actinomycete with antagonism to the root rot pathogenic bacteria and the basal rot pathogenic bacteria of wheat provided by the invention is Streptomyces miesense Q1C-5CGMCC NO. 18008.

The Streptomyces misheii Streptomyces missense Q1C-5 has been preserved in China general microbiological culture Collection center (CGMCC for short, and the address is No. 3 of Xilu No.1 of Chaoyang district, Beijing, China) in 6.21.2019, and the preservation number is NO. 18008.

The Streptomyces miyai miesense Q1C-5 is separated from sediments in the Qili sea of the North Dahe and is gram-positive bacteria. The actinomycete has gray aerial hypha on Gao's No.1 culture medium, light yellow brown hypha in the culture medium, no soluble pigment, compact spiral spore hypha, chain spore arrangement and elliptic shape. Can produce stronger cellulase, protease activity and weaker amylase activity.

The biological agent or the microbial fertilizer taking Streptomyces micionensis Q1C-5CGMCC NO.18008 as an active ingredient also belongs to the protection scope of the invention.

The actinomycetes having antagonism to the wheat root rot pathogenic bacteria and the wheat stem rot pathogenic bacteria is Streptomyces miesense Q1C-5 which is separated from sediments in the Yanglan lagoons of the North Daihe. The plate confronting experiment shows that the actinomycete has better antagonistic action on the Helminthosporium tritici causing wheat root rot and the fusarium graminearum and pseudofusarium graminearum causing wheat stem basal rot. In the confronting culture, the growth inhibition rates of the actinomycete Q1C-5 on Helminthosporium maydis, Fusarium graminearum and Fusarium pseudograminearum are 33.5%, 26.3% and 16.4%, respectively. The inhibition rate of the fermentation filtrate of Q1C-5 on spore germination of the helminthosporium park, i.e. the helminthosporium umbilicifolius is 69.0%; and can obviously cause the growth of hypha of the Helminthosporium tritici, fusarium graminearum and fusarium pseudograminearum to be abnormal and easy to break. The pot experiment shows that the actinomycete has excellent preventing and treating effect on wheat root rot caused by Helminthosporium tritici and wheat stem base rot caused by fusarium graminearum or pseudofusarium graminearum. The Streptomyces micionensis Q1C-5 is a strain with good biocontrol application prospect, and the strain is expected to provide an environment-friendly, simple and effective way for preventing and treating wheat root rot and wheat stem base rot.

Drawings

FIG. 1 shows the results of culturing Streptomyces micidaginis Q1C-5 in opposition to Helminthosporium graminearum, Fusarium graminearum and Fusarium pseudograminearum. In the figure, A is the antagonism result of Q1C-5 to Helminthosporium putrescens; b is the antagonism result of Q1C-5 to fusarium graminearum; c is the antagonistic result of Q1C-5 on Fusarium pseudograminearum.

FIG. 2 shows the results of the inhibition of the hypha and spores of pathogenic fungi of wheat by the fermentation filtrate of Streptomyces miesense Q1C-5. In the figure, A-B are hyphae of creeping cord spore of wheat root rotten Flat cord; C-D is fusarium graminearum hyphae; E-F is fusarium graminearum hyphae; G-H is creeping liriope spores of wheat root rot. A, C, E, G is control group; B. d, F, H is an experimental group.

FIG. 3 shows the control results of Streptomyces missense Q1C-5 on wheat root rot and wheat stem rot. A is wheat root rot, B is wheat stem base rot.

Detailed Description

The methods in the following examples are conventional methods unless otherwise specified.

The percentages in the following examples are by mass unless otherwise specified.

Example 1 screening for antagonism of Helminthosporium tritici, Fusarium graminearum and Fusarium pseudograminearum

Isolation and preservation of Actinomycetes

Collecting 5-10cm sample of sediment surface layer of BeiDahe Qilihai lagoons, weighing 10g sediment, placing into a triangular flask containing 90ml sterile water, shaking at 30 deg.C and 150rpm for 30min to obtain dilution of 10^-1The bacterial suspension of (4). Adding 1ml of the sediment bacterial suspension into a test tube filled with 9ml of sterile water, and uniformly mixing by vortex to obtain the dilution of 10^-2The bacterial suspension of (4); the above-mentioned method is used to make 10 times gradient dilution to 10^-4. Respectively take 10^-1To 10^-420 μ L of the bacterial suspension was uniformly spread on a Gao's No.1 medium plate (soluble starch 20g/L, KNO)₃ 1g/L，NaCl 0.5g/L，K₂HPO₄·3H₂O 0.5g/L，MgSO₄·7H₂O 0.5g/L，FeSO₄·7H₂0.5g/L of O, 15g/L of agar and 7.2-7.4 of pH; the culture medium is sterilized and K with a final concentration of 50mg/L is added₂Cr₂O₇And then inverted plate). And (3) uniformly coating a flat plate, sealing the periphery of the flat plate by using a sealing film after the surface bacterial suspension is dried, and then inversely culturing in a constant-temperature incubator at 30 ℃ for 7 d. After the bacterial colony grows out, selecting a proper dilution for counting the bacterial colony and selecting a single bacterial colony; to prevent contamination of the picked single colonies, the single colonies were streaked on PSA plates (potato 200g/L, sucrose 20g/L, agar 20g/L) and cultured at 30 ℃ for 7 days to obtain a pure culture of the bacteria. Scraping off thallus Porphyrae of actinomycetes on potato culture medium plate, placing into sterile 2ml centrifuge tube, adding CM liquid culture medium (casein amino acid 7.5g/L, yeast powder 10g/L, sodium citrate 3g/L, MgSO 2) containing sterile glycerol with final concentration of 20%₄·7H₂O 20g/L，KCl 2g/L，FeSO₄·7H₂O0.05 g/L, NaCl 50g/L), vortexThe mixture is evenly stirred by rotation and then is stored in a refrigerator at the temperature of 70 ℃ below zero. Finally, the pure cultured actinomycete 191 strain is obtained.

Secondly, screening actinomycetes for antagonizing wheat pathogenic fungi

Screening actinomycetes for antagonizing pathogenic fungi and adopting a confronting culture method. Respectively picking about 4mm of activated rhizoctonia solani, fusarium graminearum and fusarium pseudograminearum²The fungal masses of (1) were inoculated in the center of a PSA medium plate. Then preparing the actinomycetes to be detected into about 10⁶One spore/ml of the bacterial suspension was aspirated by 1. mu.l, and spotted 3cm from the center of the plate, with the end not inoculated with actinomycetes as a control. Sealing the edge of the inoculated flat plate by using a sealing film, then culturing at 30 ℃, measuring the colony radius of the pathogenic fungi at the side with the actinomycetes when the front edge hyphae of the pathogenic fungi grow to 3cm on the side without the actinomycetes, and according to the formula: the inhibition rate is [ (3 cm-pathogenic fungus colony growth radius)/3 cm]X 100%, calculating and comparing the inhibition rate of each actinomycete on pathogenic fungi. Through screening, 15 strains, 21 strains and 19 strains of actinomycetes with strong antagonism on the rhizoctonia solani, fusarium graminearum and fusarium pseudograminearum are respectively obtained. Comparison shows that an actinomycete named as Q1C-5 has strong antagonism to the three pathogenic fungi. The antagonistic action of the actinomycetes against Helminthosporium graminearum, Fusarium graminearum and Fusarium pseudograminearum is shown in FIG. 1.

Example 2 physiological and biochemical characteristics and species identification of the Q1C-5 Strain

Physiological and biochemical characteristics of strain

Observing colony color and morphology. Taking the preserved Q1C-5 strain, continuously streaking on a Gao's No.1 plate and a PSA plate respectively by using a plate streaking method, and culturing the streaked plates in an incubator at 30 ℃ in an inverted manner until a single colony appears.

A method for detecting the enzyme production of a strain. Lipase detection medium: 3g/L of beef extract, 10g/L of peptone, 6ml/L of tributyrin, 10g/L of NaCl and 20g/L of agar. A cellulose detection medium; carboxymethyl cellulose 5g/L, NaNO₃ 1g/L，K₂HPO₄2g/L，KCl 1g/L，MgSO₄.7H₂0.5g/L of O, 2g/L of yeast powder, 1g/L of glucose, 10g/L of NaCl and 20g/L of agar. Protease detection medium: 15g/L of skimmed milk powder, 2g/L of yeast powder, 10g/L of NaCl and 20g/L of agar. Amylase detection medium: 5g/L of starch, 2g/L of yeast powder, (NH)₄)₂SO₄ 1.4g/L，K₂KPO₄ 2g/L，MgSO₄·7H₂0.2g/L of O, 10g/L of NaCl and 20g/L of agar. The activated Q1C-5 strain was inoculated into the center of a lipase detection plate, a cellulase detection plate, a protease detection plate and an amylase detection plate, respectively, and was cultured in an inverted state at 30 ℃ for 5 days in an incubator. If the strain forms a transparent circle on the lipase detection medium and the protease detection medium, it indicates the production of the lipase and the protease; the cellulase detection plate is washed by 0.1 percent congo red, and if a transparent ring appears around a bacterial colony, the bacteria are shown to produce cellulase; the amylase detection plate is washed by 0.3% iodine solution, and if a transparent ring appears around a colony, the bacterium is indicated to produce amylase.

The physiological and biochemical detection results are as follows:

the strain is arranged on a Gao's No.1 culture medium, the aerial hyphae are gray, the hyphae in the medium are light yellow brown, no soluble pigment is contained, the spore hyphae are compact spiral, and the spore chain is arranged in an oval shape.

The strain can produce cellulase with higher activity, protease and amylase with lower activity; the results are shown in Table 1.

TABLE 1 enzyme production assay of Q1C-5

Class of enzyme	Enzyme production	Transparent ring diameter (cm)
			Lipase enzyme	-	0
Cellulase enzymes	+	1.97±0.03
			Protease enzyme	+	2.89±0.06
Amylase	+	0.87±0.01

Note: "+" indicates generation; "-" means not produced; the experiment was set up in triplicate.

II, 16S rRNA sequence of Q1C-5 strain and identification

Genomic DNA of actinomycetes Q1C-5 was extracted, and PCR amplification was carried out using this DNA as a template and a universal 16S rRNA primer for bacteria (upstream primer 27f: 5'-GTTTGATCCTGGCTCAG-3', downstream primer 1492r: 5'-CTACGGCTACCTTGTT-3'). And after agarose gel electrophoresis and gel cutting recovery and purification of the PCR amplification product, performing double sequencing by using 27f and 1492r primers respectively, correcting the sequence by using Chromas, and splicing by using DANMAN software to obtain a 1343bp sequence, wherein the sequence is shown as a sequence 1 in a sequence table. The sequence was aligned and analyzed in NCBI database (https:// www.ncbi.nlm.nih.gov /), and the results showed that the 16S rRNA sequence of the strain had the highest similarity to the 16S rRNA sequence of Streptomyces missense 12-4(KJ571074) in the database, reaching 99.85%.

According to the 16SrRNA sequence characteristics of the actinomycetes, the actinomycetes is named as Streptomyces missense Q1C-5, and the Streptomyces missense Q1C-5 is preserved in the general microorganism center of China Committee for culture Collection of microorganisms (CGMCC for short, with the address of No. 3 Siro 1 of the sunward area of Beijing, China) in 6.21.2019, and the preservation number is NO. 18008.

Example 3 Streptomyces mimosensis Streptomyces missense IsQ1C-5CGMCC NO.18008 application to wheat root rot Inhibiting physiological characteristics of Fusarium planopilaris, Fusarium graminearum and Fusarium pseudograminearum

Physiological characteristics of Q1C-5 in confronting culture on inhibition of wheat pathogenic fungi

According to the method described in the second step of example 1, Streptomyces missense Q1C-5 was cultured in opposition to Helminthosporium putrescentii, Fusarium graminearum and Fusarium pseudograminearum, respectively, and then cultured at 30 ℃ until the radius of colony of pathogenic fungus on the side where actinomycetes was inoculated was measured when the hyphae of the front edge of the pathogenic fungus grew to 3cm on the side where actinomycetes was not inoculated, according to the formula: the inhibition rate is [ (3 cm-pathogenic fungus colony growth radius)/3 cm ] multiplied by 100%, and the inhibition rate of the actinomycete Q1C-5 to the three pathogenic fungi is calculated; and hyphae of each pathogenic fungus in the inhibition area and the non-inhibition area are respectively picked, prepared into a piece and then are placed under a microscope for observation, and the result is shown in table 1. In order to more obviously observe the inhibition effect of Q1C-5 on pathogenic fungi, a streak-confronted culture method is adopted, and the result is shown in figure 1, so that Q1C-5 has obvious inhibition effects on the bipolaris cornucopiae, fusarium graminearum and fusarium pseudograminearum.

TABLE 1 physiological characteristics and inhibition rate of Q1C-5 bacterial colony on wheat pathogenic fungi

From the results, in the confronting culture process, the actinomycete Q1C-5 has obvious physiological inhibition characteristics on the bipolaris cornucopiae, fusarium graminearum and fusarium pseudograminearum, and the inhibition rates are 33.5%, 26.3% and 16.4% respectively.

Secondly, the fermentation liquor of Q1C-5 inhibits the hypha and the spore of wheat pathogenic fungi

And preparing Q1C-5 fermentation liquor. 10ml of PSA liquid medium was filled into a test tube, sterilized, and inoculated with a loop of Q1C-5 actinomycetes spores, which were cultured at 150rpm for 5 days on a constant temperature shaker at 30 ℃. Then sucking the liquid fermentation product, and filtering with a sterile filter membrane to obtain a fermentation filtrate of Q1C-5.

And (3) preparing a pathogenic fungus hypha suspension. Cut about 1cm with a sterile scalpel²The pathogenic fungi growing on the PSA plate are then placed into a 2ml centrifuge tube filled with 1ml of sterile water, hyphae on the fungus blocks are scraped by an inoculating loop, the agar blocks are taken out, and then vortex and shake uniformly to respectively obtain hypha suspensions of the bipolaris cornuta, fusarium graminearum and fusarium pseudograminearum.

Preparation of a pathogenic fungal spore suspension. Since fusarium graminearum and fusarium pseudograminearum do not produce spores on PSA plates, this experiment was only directed to bipolaris meretrix which produces spores on PSA plates. Adding 5ml of sterile water on a flat plate with the creeping liriope muscari with the wheat root, gently scraping hypha and spores, then sucking the bacterial suspension, and filtering by sterile lens wiping paper to obtain the spore suspension of the creeping liriope muscari with the wheat root.

Respectively sucking 250 mu l of fermentation filtrate of Q1C-5 and 50 mu l of hypha suspension or spore suspension of pathogenic fungi, adding into a 2ml centrifuge tube, and mixing to obtain an experimental group; meanwhile, a control group is set, and 250 mul of the filtered PSA liquid culture medium and 50 mul of hypha suspension or spore suspension of the pathogenic fungi are sucked and mixed. The mixed solution of the experimental combination control group is cultured in an incubator at 30 ℃ for 6h, 10 mu l of culture solution is respectively sucked from a centrifuge tube to be made into slices, and the influence of the fermentation filtrate of Q1C-5 on the germination of pathogenic fungi hyphae and spores is observed under an optical microscope, and the result is shown in a figure 2 and a table 2.

TABLE 2 inhibition of wheat pathogenic fungi hyphae and spores by fermentation filtrates of Q1C-5

Note: "-" was not detected because the pathogen did not sporulate on the PSA plates.

From the above results, it can be seen that the fermentation filtrate of Q1C-5 can obviously inhibit the extension of pathogenic fungi hypha, so that the hypha of pathogenic fungi is deformed and easily broken; the inhibition rate of spore germination of the bipolaris tritici-merrill reaches 69.0%, and spore germ tubes are thick, short and slow in extension.

Example 4 Streptomyces mimosensis Streptomyces missense Q1C-5CGMCC NO.18008 application to wheat root Control effect of rot and wheat stem base rot

Preparation of soil containing pathogenic fungi

Propagation of pathogenic fungi. Pathogenic fungi 4cm²Washing thallus on the bacterium block, inoculating the thallus on a millet culture medium (200 g of millet is put into boiling water to be boiled for 2min, then the millet is poured out, is quickly cooled and washed for 3-4 times by cold water, after being washed, the millet is put on newspaper and a ventilation port to be dried until the surface is anhydrous, then is transferred into a 500ml triangular flask, is sterilized for 30min at 121 ℃, then is taken out and cooled for standby), putting the millet culture medium into an incubator at 30 ℃ for culture, and shaking for 2-3 times every day to ensure that pathogenic fungi grow uniformly; and culturing for 7 days for later use.

And (4) preparing garden soil. Taking a plurality of soils in garden of China geological university (Beijing), grinding, crushing, sieving to make the soils uniform, then filling into a triangular flask, performing high-pressure steam sterilization for 1h, and cooling for later use.

And (5) preparing soil. The preparation of the soil is provided with three treatments of a blank group, a control group and an experimental group. 100g of sterilized soil was filled into a plastic cup as a blank (the plastic cup was previously drilled with 5 small holes of equivalent diameter uniformly in the bottom thereof); the pathogenic fungi propagated by the millet culture medium and the sterilized soil are mixed in a ratio of 0.5: uniformly mixing at a ratio of 100, and transferring the uniformly mixed bacteria and soil into a plastic cup to stand up against the group; the pathogenic fungi propagated by actinomycetes Q1C-5 and a millet culture medium and sterilized soil are mixed in a ratio of 0.1: 0.5: 100, and transferring the mixed bacteria and soil into a plastic cup to serve as an experimental group. Three replicates of each treatment were performed.

Second, experiment of potting

Preparing wheat seeds. Soaking the wheat seeds overnight, then accelerating germination, and after the seeds are exposed to white, selecting the wheat seeds with full grains, similar germination degrees and similar sizes for later use.

And (5) sowing and managing. The seeds after pregermination were sown in plastic cups of blank group, control group and experimental group, 20 seeds/cup, respectively. Each treatment was incubated at room temperature and watered by pouring water from the bottom, once every 5 days, with 40ml each time.

And (5) result and statistics. After the wheat had grown to 20 days, the soil was removed from the plastic cups and the soil on the wheat roots was washed away (fig. 3), and the root length and plant height of each wheat corresponding to each treatment were evaluated comprehensively, and the results are shown in table 3.

TABLE 3 prevention and treatment effects of Q1C-5 on wheat root rot and wheat stem base rot

From the above results, it can be seen that the wheat in the blank group had good growth state and flourished growth due to its growth in the sterilized soil; the wheat in the control group is grown in the diseased soil containing Helminthosporium tritici or Fusarium graminearum, so that the wheat is obviously diseased, and the stem length and the root length of the wheat are obviously shortened compared with those of the blank group. The growth state of wheat in the experimental group is between the blank group and the control group due to the growth of wheat in the diseased soil added with actinomycetes Q1C-5 spores, and the stem length and the root length of the wheat are obviously increased and extended compared with the control group. Experimental results show that the actinomycete Q1C-5 is applied in the soil in advance, and the wheat root rot caused by the Helminthosporium tritici can be effectively prevented and controlled to a certain extent. In contrast, the wheat stalk base rot caused by fusarium graminearum is relatively severe in onset, and in the diseased soil containing fusarium graminearum, both the stalk length and the root length are significantly affected, particularly the root elongation is significantly reduced, but when actinomycetes Q1C-5 spores are added into the diseased soil, both the stalk length and the root length of wheat are slightly increased compared with those of a control group, but the effect is weaker than that of the control group. The experimental results show that the actinomycete Q1C-5 as a biological agent has obvious effect on preventing and treating the wheat root rot and also has a certain degree of preventing and treating effect on the wheat stem basal rot to a certain degree.

The foregoing description is intended to be illustrative rather than limiting, and it will be appreciated by those skilled in the art that many modifications, variations or equivalents may be made without departing from the spirit and scope of the invention as defined in the appended claims.

Sequence listing

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<120> streptomyces micusii and application thereof in prevention and treatment of wheat root rot and stem basal rot

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tctgcagtcg atacgggcag gctagagttc ggtaggggag atcggaattc ctggtgtagc 600

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ccgtaaacgg tgggcactag gtgtgggcaa cattccacgt tgtccgtgcc gcagctaacg 780

cattaagtgc cccgcctggg gagtacggcc gcaaggctaa aactcaaagg aattgacggg 840

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Claims

1. Streptomyces misoniensisStreptomyces misionensisCharacterized in that the name is Streptomyces maireiStreptomyces misionensis Q1C-5, wherein the preservation number of the general microbiological center of China Committee for culture Collection of microorganisms is CGMCC number 18008.

2. Rice crackerStreptomyceteStreptomyces misionensis The application of Q1C-5CGMCC number 18008 in preventing and treating plant diseases; the plant diseases are wheat root rot caused by wheat root rot and fusarium graminearum or pseudofusarium graminearum.

3. Streptomyces misoensisStreptomyces misionensis Application of Q1C-5CGMCC number 18008 in preparing biocontrol microbial inoculum or microbial fertilizer for plant pathogenic fungi.

4. The biocontrol microbial inoculum for preventing and treating plant diseases is characterized in that the active ingredient of the biocontrol microbial inoculum is streptomyces maireiStreptomyces misionensis Q1C-5 CGMCC No. 18008。

5. The microbial fertilizer for preventing and treating plant pathogenic fungi is characterized in that the active ingredient of the microbial fertilizer is streptomyces micelliiStreptomyces misionensis Q1C-5 CGMCC No. 18008。