CN111500501A

CN111500501A - Streptomyces misonii strain and application thereof in preventing and treating wheat root rot and stem basal rot

Info

Publication number: CN111500501A
Application number: CN202010376134.9A
Authority: CN
Inventors: 魏士平; 卞佳琪
Original assignee: China University of Geosciences Beijing
Current assignee: China University of Geosciences Beijing
Priority date: 2020-05-07
Filing date: 2020-05-07
Publication date: 2020-08-07
Anticipated expiration: 2040-05-07
Also published as: CN111500501B

Abstract

The invention discloses an actinomycete with antagonistic action on wheat root rot pathogenic bacteria and stem rot pathogenic bacteria and application thereof. The actinomycete is Streptomyces missense Q1C-5CGMCC NO. 18008. The compound preparation has antagonistic action on rhizoctonia solani (Bipolaris sorokiniana), Fusarium graminearum (Fusarium graminearum) and Fusarium pseudograminearum, can effectively inhibit hypha elongation and spore germination of pathogenic fungi, and can be used for preventing and treating wheat root rot caused by the rhizoctonia solani and wheat stalk rot caused by the Fusarium pseudograminearum.

Description

Streptomyces misonii strain and application thereof in preventing and treating wheat root rot and stem basal rot

Technical Field

The invention relates to streptomyces mairei with antagonistic effect on wheat root rot cells, fusarium graminearum and fusarium pseudograminearum and application thereof in preventing and treating wheat root rot and wheat stem rot.

Background

Along with the improvement of living standard of people, people pay more and more attention to a green agriculture development mode, namely, the agriculture development mode which can protect the environment and ensure green and pollution-free agricultural products while promoting the agriculture development. However, in the actual production process of China, due to continuous cropping of crops, certain pathogenic bacteria attack in successive years to form diseased soil; in addition, due to the reduction of organic matters in soil caused by long-term application of fertilizers and pesticides, the microbial ecology of the soil is destroyed, so that soil-borne diseases are aggravated in successive years. Although people obtain certain effect through chemical prevention and control technology, long-term use of chemical preparations also destroys the soil aggregate structure and physicochemical properties, further aggravates the soil ecological environment, and simultaneously causes a series of problems of crop drug resistance accumulation, agricultural product drug residue and the like. The beneficial microorganisms are utilized to improve the biological community in the soil, eliminate or inhibit pathogenic bacteria in the soil, reduce the use of pesticides and restore the soil ecosystem, which is a necessary way for the development of green agriculture in the future.

Biological control measures for controlling plant diseases by using beneficial microorganisms have become mainstream of research and application due to the characteristics of safety, low toxicity, environmental friendliness and the like. Actinomycetes are an important microbial resource, can produce various active compounds, and are used as biocontrol bacteria for preventing and treating agricultural diseases and insect pests. In recent years, some scholars have been searching for new actinomycete resources due to the difficulty in finding more novel drugs and bioactive substances from terrestrial actinomycetes. The particularity of marine environment determines that the structure and bioactive substances of secondary metabolites generated by marine actinomycetes are different from those of terrestrial actinomycetes, so that the marine actinomycetes has more diversity. The marine actinomycete as one new field can produce various compounds with novel structure, has obvious research and development prospects on bacteriostatic activity and disease resistance mechanism, and can produce various secondary metabolites as bioactive compounds, especially streptomyces. At present, various bioactive natural products such as alkaloids, polyketones, peptides, macrolides and the like are separated from marine actinomycetes, and can resist bacteria, fungi and tumors or have cytotoxic activity and the like. The invention takes the actinomycetes in the sea as a research object, and prepares the actinomycetes into a microbial preparation for green agricultural production through the experiments of separation and screening of the actinomycetes, prevention and treatment of diseases and the like, thereby having wide development prospect.

Wheat is a gramineous crop widely planted around the world and is also one of the main food crops in China. The method is mainly centralized in winter wheat areas at the middle and lower reaches of Yangtze river, northeast spring wheat area, northwest spring wheat area, southwest wheat area and Qinghai-Tibet plateau winter spring wheat area, wherein the wheat yield of the three provinces of Henan, Shandong and Hebei accounts for more than 50% of the total wheat yield of the whole country every year. Plant diseases are the most important reason for limiting the yield increase of wheat, and the diseases harmful to wheat in China mainly comprise: stripe rust, leaf rust, stem rust, stinking smut, loose smut, yellow dwarf, red dwarf, take-all, leaf spot and the like of wheat. The wheat root rot and stem base rot occur in the northeast, northwest and inner Mongolia wheat areas of China, which can cause large area yield reduction of wheat. But because the disease belongs to soil-borne diseases, the chemical prevention and control method is difficult to work; moreover, the use of chemical agents also leads to increased resistance of pathogenic bacteria and thus to environmental pollution. Therefore, the application of green pollution-free biological control to control wheat soil-borne diseases is the direction of future development.

Disclosure of Invention

The invention aims to provide an actinomycete with antagonistic action on wheat root rot pathogenic bacteria and wheat stem base rot pathogenic bacteria and application thereof in preventing and treating wheat root rot and wheat stem base rot.

The actinomycete with antagonism to the root rot pathogenic bacteria and the basal rot pathogenic bacteria of wheat provided by the invention is Streptomyces miesense Q1C-5CGMCC NO. 18008.

The Streptomyces misheii Streptomyces missense Q1C-5 has been preserved in China general microbiological culture Collection center (CGMCC for short, and the address is No. 3 of Xilu No.1 of Chaoyang district, Beijing, China) in 6.21.2019, and the preservation number is NO. 18008.

The Streptomyces miyai miesense Q1C-5 is separated from sediments in the Qili sea of the North Dahe and is gram-positive bacteria. The actinomycete has gray aerial hypha on Gao's No.1 culture medium, light yellow brown hypha in the culture medium, no soluble pigment, compact spiral spore hypha, chain spore arrangement and elliptic shape. Can produce stronger cellulase, protease activity and weaker amylase activity.

The biological agent or the microbial fertilizer taking Streptomyces micionensis Q1C-5CGMCC NO.18008 as an active ingredient also belongs to the protection scope of the invention.

The actinomycetes having antagonism to the wheat root rot pathogenic bacteria and the wheat stem rot pathogenic bacteria is Streptomyces miesense Q1C-5 which is separated from sediments in the Yanglan lagoons of the North Daihe. The plate confronting experiment shows that the actinomycete has better antagonistic action on the Helminthosporium tritici causing wheat root rot and the fusarium graminearum and pseudofusarium graminearum causing wheat stem basal rot. In the confronting culture, the growth inhibition rates of the actinomycete Q1C-5 on Helminthosporium maydis, Fusarium graminearum and Fusarium pseudograminearum are 33.5%, 26.3% and 16.4%, respectively. The inhibition rate of the fermentation filtrate of Q1C-5 on spore germination of the helminthosporium park, i.e. the helminthosporium umbilicifolius is 69.0%; and can obviously cause the growth of hypha of the Helminthosporium tritici, fusarium graminearum and fusarium pseudograminearum to be abnormal and easy to break. The pot experiment shows that the actinomycete has excellent preventing and treating effect on wheat root rot caused by Helminthosporium tritici and wheat stem base rot caused by fusarium graminearum or pseudofusarium graminearum. The Streptomyces micionensis Q1C-5 is a strain with good biocontrol application prospect, and the strain is expected to provide an environment-friendly, simple and effective way for preventing and treating wheat root rot and wheat stem base rot.

Drawings

FIG. 1 shows the results of culturing Streptomyces micidaginis Q1C-5 in opposition to Helminthosporium graminearum, Fusarium graminearum and Fusarium pseudograminearum. In the figure, A is the antagonism result of Q1C-5 to Helminthosporium putrescens; b is the antagonism result of Q1C-5 to fusarium graminearum; c is the antagonistic result of Q1C-5 on Fusarium pseudograminearum.

FIG. 2 shows the results of the inhibition of the hypha and spores of pathogenic fungi of wheat by the fermentation filtrate of Streptomyces miesense Q1C-5. In the figure, A-B are hyphae of creeping cord spore of wheat root rotten Flat cord; C-D is fusarium graminearum hyphae; E-F is fusarium graminearum hyphae; G-H is creeping liriope spores of wheat root rot. A, C, E, G is control group; B. d, F, H is an experimental group.

FIG. 3 shows the control results of Streptomyces missense Q1C-5 on wheat root rot and wheat stem rot. A is wheat root rot, B is wheat stem base rot.

Detailed Description

The methods in the following examples are conventional methods unless otherwise specified.

The percentages in the following examples are by mass unless otherwise specified.

Example 1 screening for antagonism of Helminthosporium tritici, Fusarium graminearum and Fusarium pseudograminearum

Isolation and preservation of Actinomycetes

Collecting 5-10cm sample of sediment surface layer of BeiDahe Qilihai lagoons, weighing 10g sediment, placing into a triangular flask containing 90ml sterile water, shaking at 30 deg.C and 150rpm for 30min to obtain dilution of 10^-1The bacterial suspension of (4). Adding 1ml of the sediment bacterial suspension into a test tube filled with 9ml of sterile water, and uniformly mixing by vortex to obtain the dilution of 10^-2The bacterial suspension of (4); the above-mentioned method is used to make 10 times gradient dilution to 10^-4. Respectively take 10^-1To 10^-420. mu.l of the bacterial suspension was uniformly spread on a Gao's No.1 medium plate (soluble starch 20 g/L)₃1g/L，NaCl 0.5g/L，K₂HPO₄·3H₂O 0.5g/L，MgSO₄·7H₂O0.5g/L，FeSO₄·7H₂0.5 g/L O, 15 g/L agar, pH 7.2-7.4, and K with the final concentration of 50 mg/L added after the culture medium is sterilized₂Cr₂O₇Then pouring the plate), coating the uniform plate, sealing the periphery of the plate by using a sealing film after the surface bacterial suspension is dried, then carrying out inverted culture in a constant temperature incubator at 30 ℃ for 7d, selecting proper dilution for counting bacterial colonies and picking single bacterial colonies after the bacterial colonies grow out, carrying out streak purification on the picked single bacterial colonies on a PSA plate (potato 200 g/L, cane sugar 20 g/L, agar 20 g/L) to prevent the picked single bacterial colonies from mixing, and carrying out culture at 30 DEGCulturing for 7 days to obtain pure culture of bacteria, scraping off thallus Porphyrae of actinomycetes on potato culture medium plate, placing into sterile 2ml centrifuge tube, and adding CM liquid culture medium (casein amino acid 7.5 g/L, yeast powder 10 g/L, sodium citrate 3 g/L) containing sterile glycerol with final concentration of 20%₄·7H₂O 20g/L，KCl 2g/L，FeSO₄·7H₂O0.05 g/L50 g/L), vortexing and shaking uniformly, and then placing in a refrigerator at-70 ℃ for storage to obtain the pure cultured actinomycete 191 strains.

Secondly, screening actinomycetes for antagonizing wheat pathogenic fungi

Screening actinomycetes for antagonizing pathogenic fungi and adopting a confronting culture method. Respectively picking about 4mm of activated rhizoctonia solani, fusarium graminearum and fusarium pseudograminearum²The fungal masses of (1) were inoculated in the center of a PSA medium plate. Then preparing the actinomycetes to be detected into about 10⁶One spore/ml of the bacterial suspension was aspirated by 1. mu.l, and spotted 3cm from the center of the plate, with the end not inoculated with actinomycetes as a control. Sealing the edge of the inoculated flat plate by using a sealing film, then culturing at 30 ℃, measuring the colony radius of the pathogenic fungi at the side with the actinomycetes when the front edge hyphae of the pathogenic fungi grow to 3cm on the side without the actinomycetes, and according to the formula: the inhibition rate is [ (3 cm-pathogenic fungus colony growth radius)/3 cm]× 100%, calculating and comparing the inhibition rate of each actinomycete to pathogenic fungi, through screening, there are 15, 21 and 19 actinomycetes with strong antagonism to Helminthosporium graminearum, Fusarium graminearum and Fusarium pseudograminearum respectively, through comparison, it is found that one actinomycete named Q1C-5 shows strong antagonism to the above three pathogenic fungi, the antagonism of the actinomycete to Helminthosporium graminearum, Fusarium graminearum and Fusarium pseudograminearum is shown in figure 1.

Example 2 physiological and biochemical characteristics and species identification of the Q1C-5 Strain

Physiological and biochemical characteristics of strain

Observing colony color and morphology. Taking the preserved Q1C-5 strain, continuously streaking on a Gao's No.1 plate and a PSA plate respectively by using a plate streaking method, and culturing the streaked plates in an incubator at 30 ℃ in an inverted manner until a single colony appears.

Lipase detection culture medium including beef extract 3 g/L, peptone 10 g/L, tributyrin 6 ml/L10 g/L, agar 20 g/L, cellulose detection culture medium, and carboxymethyl cellulose 5 g/L₃1g/L，K₂HPO₄2g/L，KCl 1g/L，MgSO₄.7H₂O0.5 g/L, yeast powder 2 g/L, glucose 1 g/L0, NaCl10 g/L, agar 20 g/L, protease detection culture medium including skimmed milk powder 15 g/L, yeast powder 2 g/L10 g/L, agar 20 g/L, amylase detection culture medium including starch 5 g/L, yeast powder 2 g/L, NH₄)₂SO₄1.4g/L，K₂KPO₄2g/L，MgSO₄·7H₂O0.2 g/L10 g/L and agar 20 g/L, respectively inoculating the activated strain Q1C-5 to the centers of a lipase detection plate, a cellulase detection plate, a protease detection plate and an amylase detection plate, and carrying out inverted culture in an incubator at 30 ℃ for 5d, wherein if a macroscopic transparent ring is formed on a lipase detection culture medium and a protease detection culture medium by the strain, the lipase and the protease are produced, the cellulase detection plate is washed by 0.1% congo red, if a transparent ring is formed around a colony, the cellulase is produced by the strain, and the amylase detection plate is washed by 0.3% iodine solution, if a transparent ring is formed around the colony, the amylase is produced by the strain.

The physiological and biochemical detection results are as follows:

the strain is arranged on a Gao's No.1 culture medium, the aerial hyphae are gray, the hyphae in the medium are light yellow brown, no soluble pigment is contained, the spore hyphae are compact spiral, and the spore chain is arranged in an oval shape.

The strain can produce cellulase with higher activity, protease and amylase with lower activity; the results are shown in Table 1.

TABLE 1 enzyme production assay of Q1C-5

Class of enzyme	Enzyme production	Transparent ring diameter (cm)
			Lipase enzyme	-	0
Cellulase enzymes	+	1.97±0.03
			Protease enzyme	+	2.89±0.06
Amylase	+	0.87±0.01

Note: "+" indicates generation; "-" means not produced; the experiment was set up in triplicate.

II, 16S rRNA sequence of Q1C-5 strain and identification

Genomic DNA of actinomycetes Q1C-5 was extracted, and PCR amplification was carried out using this DNA as a template and a universal 16S rRNA primer for bacteria (upstream primer 27f: 5'-GTTTGATCCTGGCTCAG-3', downstream primer 1492r: 5'-CTACGGCTACCTTGTT-3'). And after agarose gel electrophoresis and gel cutting recovery and purification of the PCR amplification product, performing double sequencing by using 27f and 1492r primers respectively, correcting the sequence by using Chromas, and splicing by using DANMAN software to obtain a 1343bp sequence, wherein the sequence is shown as a sequence 1 in a sequence table. The sequence was aligned and analyzed in NCBI database (https:// www.ncbi.nlm.nih.gov /), and the results showed that the 16S rRNA sequence of the strain had the highest similarity to the 16S rRNA sequence of Streptomyces missense 12-4(KJ571074) in the database, reaching 99.85%.

According to the 16SrRNA sequence characteristics of the actinomycetes, the actinomycetes is named as Streptomyces missense Q1C-5, and the Streptomyces missense Q1C-5 is preserved in the general microorganism center of China Committee for culture Collection of microorganisms (CGMCC for short, with the address of No. 3 Siro 1 of the sunward area of Beijing, China) in 6.21.2019, and the preservation number is NO. 18008.

Example 3 Streptomyces mimosensis Streptomyces missense IsQ1C-5CGMCC NO.18008 application to wheat root rot Inhibiting physiological characteristics of Fusarium planopilaris, Fusarium graminearum and Fusarium pseudograminearum

Physiological characteristics of Q1C-5 in confronting culture on inhibition of wheat pathogenic fungi

According to the method described in the second step of example 1, Streptomyces misensisis Q1C-5 is respectively cultured in opposition to september gracilis, fusarium graminearum and fusarium pseudograminearum, then cultured at 30 ℃, when the front edge hyphae of the pathogenic fungi grows to 3cm on the side not connected with the actinomycetes, the colony radius of the pathogenic fungi on the side connected with the actinomycetes at the moment is measured, the inhibition ratio of the pathogenic fungi on the side connected with the actinomycetes is calculated according to the formula of (3 cm-pathogenic fungi colony growth radius)/3 cm) × 100% and the inhibition ratios of the actinomycetes Q1C-5 to the three pathogenic fungi are calculated, and the hyphae of each pathogenic fungus in the inhibition area and the non-inhibition area are respectively picked and made into a piece, and then placed under a microscope for observation, the result is shown in table 1, in order to more obviously observe the inhibition effect of the Q1C-5 on the pathogenic fungi, the streak opposition culture method is adopted, and the result is shown in fig. 1, and Q1-1C-5 has the effect on the fusarium pseudogramineae and the fusarium pseudogramineae.

TABLE 1 physiological characteristics and inhibition rate of Q1C-5 bacterial colony on wheat pathogenic fungi

From the results, in the confronting culture process, the actinomycete Q1C-5 has obvious physiological inhibition characteristics on the bipolaris cornucopiae, fusarium graminearum and fusarium pseudograminearum, and the inhibition rates are 33.5%, 26.3% and 16.4% respectively.

Secondly, the fermentation liquor of Q1C-5 inhibits the hypha and the spore of wheat pathogenic fungi

And preparing Q1C-5 fermentation liquor. 10ml of PSA liquid medium was filled into a test tube, sterilized, and inoculated with a loop of Q1C-5 actinomycetes spores, which were cultured at 150rpm for 5 days on a constant temperature shaker at 30 ℃. Then sucking the liquid fermentation product, and filtering with a sterile filter membrane to obtain a fermentation filtrate of Q1C-5.

And (3) preparing a pathogenic fungus hypha suspension. Cut about 1cm with a sterile scalpel²The pathogenic fungi growing on the PSA plate are then placed into a 2ml centrifuge tube filled with 1ml of sterile water, hyphae on the fungus blocks are scraped by an inoculating loop, the agar blocks are taken out, and then vortex and shake uniformly to respectively obtain hypha suspensions of the bipolaris cornuta, fusarium graminearum and fusarium pseudograminearum.

Preparation of a pathogenic fungal spore suspension. Since fusarium graminearum and fusarium pseudograminearum do not produce spores on PSA plates, this experiment was only directed to bipolaris meretrix which produces spores on PSA plates. Adding 5ml of sterile water on a flat plate with the creeping liriope muscari with the wheat root, gently scraping hypha and spores, then sucking the bacterial suspension, and filtering by sterile lens wiping paper to obtain the spore suspension of the creeping liriope muscari with the wheat root.

Respectively sucking 250 mu l of fermentation filtrate of Q1C-5 and 50 mu l of hypha suspension or spore suspension of pathogenic fungi, adding into a 2ml centrifuge tube, and mixing to obtain an experimental group; meanwhile, a control group is set, and 250 mul of the filtered PSA liquid culture medium and 50 mul of hypha suspension or spore suspension of the pathogenic fungi are sucked and mixed. The mixed solution of the experimental combination control group is cultured in an incubator at 30 ℃ for 6h, 10 mu l of culture solution is respectively sucked from a centrifuge tube to be made into slices, and the influence of the fermentation filtrate of Q1C-5 on the germination of pathogenic fungi hyphae and spores is observed under an optical microscope, and the result is shown in a figure 2 and a table 2.

TABLE 2 inhibition of wheat pathogenic fungi hyphae and spores by fermentation filtrates of Q1C-5

Note: "-" was not detected because the pathogen did not sporulate on the PSA plates.

From the above results, it can be seen that the fermentation filtrate of Q1C-5 can obviously inhibit the extension of pathogenic fungi hypha, so that the hypha of pathogenic fungi is deformed and easily broken; the inhibition rate of spore germination of the bipolaris tritici-merrill reaches 69.0%, and spore germ tubes are thick, short and slow in extension.

Example 4 Streptomyces mimosensis Streptomyces missense Q1C-5CGMCC NO.18008 application to wheat root Control effect of rot and wheat stem base rot

Preparation of soil containing pathogenic fungi

Propagation of pathogenic fungi. Pathogenic fungi 4cm²Washing thallus on the bacterium block, inoculating the thallus on a millet culture medium (200 g of millet is put into boiling water to be boiled for 2min, then the millet is poured out, is quickly cooled and washed for 3-4 times by cold water, after being washed, the millet is put on newspaper and a ventilation port to be dried until the surface is anhydrous, then is transferred into a 500ml triangular flask, is sterilized for 30min at 121 ℃, then is taken out and cooled for standby), putting the millet culture medium into an incubator at 30 ℃ for culture, and shaking for 2-3 times every day to ensure that pathogenic fungi grow uniformly; and culturing for 7 days for later use.

And (4) preparing garden soil. Taking a plurality of soils in garden of China geological university (Beijing), grinding, crushing, sieving to make the soils uniform, then filling into a triangular flask, performing high-pressure steam sterilization for 1h, and cooling for later use.

And (5) preparing soil. The preparation of the soil is provided with three treatments of a blank group, a control group and an experimental group. 100g of sterilized soil was filled into a plastic cup as a blank (the plastic cup was previously drilled with 5 small holes of equivalent diameter uniformly in the bottom thereof); the pathogenic fungi propagated by the millet culture medium and the sterilized soil are mixed in a ratio of 0.5: uniformly mixing at a ratio of 100, and transferring the uniformly mixed bacteria and soil into a plastic cup to stand up against the group; the pathogenic fungi propagated by actinomycetes Q1C-5 and a millet culture medium and sterilized soil are mixed in a ratio of 0.1: 0.5: 100, and transferring the mixed bacteria and soil into a plastic cup to serve as an experimental group. Three replicates of each treatment were performed.

Second, experiment of potting

Preparing wheat seeds. Soaking the wheat seeds overnight, then accelerating germination, and after the seeds are exposed to white, selecting the wheat seeds with full grains, similar germination degrees and similar sizes for later use.

And (5) sowing and managing. The seeds after pregermination were sown in plastic cups of blank group, control group and experimental group, 20 seeds/cup, respectively. Each treatment was incubated at room temperature and watered by pouring water from the bottom, once every 5 days, with 40ml each time.

And (5) result and statistics. After the wheat had grown to 20 days, the soil was removed from the plastic cups and the soil on the wheat roots was washed away (fig. 3), and the root length and plant height of each wheat corresponding to each treatment were evaluated comprehensively, and the results are shown in table 3.

TABLE 3 prevention and treatment effects of Q1C-5 on wheat root rot and wheat stem base rot

From the above results, it can be seen that the wheat in the blank group had good growth state and flourished growth due to its growth in the sterilized soil; the wheat in the control group is grown in the diseased soil containing Helminthosporium tritici or Fusarium graminearum, so that the wheat is obviously diseased, and the stem length and the root length of the wheat are obviously shortened compared with those of the blank group. The growth state of wheat in the experimental group is between the blank group and the control group due to the growth of wheat in the diseased soil added with actinomycetes Q1C-5 spores, and the stem length and the root length of the wheat are obviously increased and extended compared with the control group. Experimental results show that the actinomycete Q1C-5 is applied in the soil in advance, and the wheat root rot caused by the Helminthosporium tritici can be effectively prevented and controlled to a certain extent. In contrast, the wheat stalk base rot caused by fusarium graminearum is relatively severe in onset, and in the diseased soil containing fusarium graminearum, both the stalk length and the root length are significantly affected, particularly the root elongation is significantly reduced, but when actinomycetes Q1C-5 spores are added into the diseased soil, both the stalk length and the root length of wheat are slightly increased compared with those of a control group, but the effect is weaker than that of the control group. The experimental results show that the actinomycete Q1C-5 as a biological agent has obvious effect on preventing and treating the wheat root rot and also has a certain degree of preventing and treating effect on the wheat stem basal rot to a certain degree.

The foregoing description is intended to be illustrative rather than limiting, and it will be appreciated by those skilled in the art that many modifications, variations or equivalents may be made without departing from the spirit and scope of the invention as defined in the appended claims.

Sequence listing

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<120> streptomyces micusii and application thereof in prevention and treatment of wheat root rot and stem basal rot

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<170>PatentIn version 3.5

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Claims

1. The Streptomyces miesense is characterized by being named as Streptomyces miesense Q1C-5, and the preservation number of the Streptomyces miesense in China general microbiological culture Collection center is CGMCC No. 18008.

2. The application of Streptomyces missense Q1C-5CGMCC No.18008 in preventing and treating plant diseases; the plant diseases are wheat root rot caused by wheat root rot and fusarium graminearum or pseudofusarium graminearum.

3. Use according to claim 2, characterized in that the plant diseases are diseases occurring on wheat.

4. Application of Streptomyces miesense Q1C-5CGMCC No.18008 in preparation of biocontrol microbial inoculum or microbial fertilizer for plant pathogenic fungi.

5. The biocontrol microbial inoculum for preventing and treating plant diseases is characterized in that the active ingredient of the biocontrol microbial inoculum is Streptomyces miesense Q1C-5CGMCC No. 18008.

6. The biocontrol microbial inoculum of claim 5 wherein the plant diseases are wheat root rot caused by wheat root rot cells and wheat stem rot caused by fusarium graminearum or pseudofusarium graminearum.

7. The microbial fertilizer for preventing and treating plant pathogenic fungi is characterized in that the active ingredient of the microbial fertilizer is Streptomyces micusiensis Q1C-5CGMCC No. 18008.

8. The microbial fertilizer according to claim 7, wherein the plant diseases are wheat root rot caused by wheat root rot cells and wheat stalk rot caused by fusarium graminearum or fusarium pseudograminearum.