CN111500450A - Cell culture is with shaking mixing arrangement - Google Patents
Cell culture is with shaking mixing arrangement Download PDFInfo
- Publication number
- CN111500450A CN111500450A CN202010353804.5A CN202010353804A CN111500450A CN 111500450 A CN111500450 A CN 111500450A CN 202010353804 A CN202010353804 A CN 202010353804A CN 111500450 A CN111500450 A CN 111500450A
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- Prior art keywords
- cylinder
- rack
- connecting rod
- shaking
- cell culture
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/16—Vibrating; Shaking; Tilting
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
- B01F31/20—Mixing the contents of independent containers, e.g. test tubes
- B01F31/201—Holders therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
- B01F35/30—Driving arrangements; Transmissions; Couplings; Brakes
- B01F35/31—Couplings
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F2101/00—Mixing characterised by the nature of the mixed materials or by the application field
- B01F2101/23—Mixing of laboratory samples e.g. in preparation of analysing or testing properties of materials
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- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Sustainable Development (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Mixers With Rotating Receptacles And Mixers With Vibration Mechanisms (AREA)
Abstract
The invention provides a cell culture oscillation mixing device, which comprises a fixed table, an oscillation cylinder, a sample cylinder, a first oscillation mechanism and a second oscillation mechanism, wherein the fixed table is fixedly connected with the oscillation cylinder; a rotary table is rotatably arranged on the fixed table through a rotating shaft; the vibration cylinder is fixedly arranged on the rotary table, the sample cylinder is arranged in the vibration cylinder, and two opposite sides of the sample cylinder are provided with support shafts which are rotationally connected with the vibration cylinder; the first oscillating mechanism is arranged on the fixed table and used for driving the rotary table to rotate forward and backward alternately; the second vibrates the mechanism and installs in vibrating a section of thick bamboo for drive a sample section of thick bamboo in vibrating a section of thick bamboo swing back and forth. According to the invention, the first oscillating mechanism and the second oscillating mechanism are arranged, so that the sample cylinder rotates back and forth around the central axis of the oscillating cylinder, and swings back and forth around the central axis of the supporting shaft, thereby improving the oscillating and mixing effect; the invention can adjust the amplitude of the sample cylinder for shaking uniformly by controlling the first shaking mechanism and the second shaking mechanism, and is beneficial to improving the cell culture effect.
Description
Technical Field
The invention relates to the technical field of cell culture, in particular to a shaking and mixing device for cell culture.
Background
In the experiment of carrying out the medicine to cell growth influence, need carry out the mixing to the culture solution of cell growth environment the inside at interval, and make medicine and cell and culture solution and cell fully mix to prevent because of the time is longer, and make the material in medicine or the culture solution can not realize carrying out abundant contact to the cell, will lead to experimental data deviation. However, in the actual operation process, if manual blending is not performed, only natural diffusion blending is relied on, so that sufficient blending is not easy, and the accuracy of data is influenced due to long time.
At present, in the cell culture process, the aim of uniformly mixing is difficult to achieve by manually shaking the culture bottle or the culture plate, the experimental workload is increased, and the experimental time is prolonged.
Disclosure of Invention
Based on the technical problems in the background art, the invention provides a shaking and mixing device for cell culture.
The invention provides a cell culture oscillation mixing device, which comprises a fixed table, an oscillation cylinder, a sample cylinder, a first oscillation mechanism and a second oscillation mechanism, wherein the fixed table is fixedly connected with the oscillation cylinder; the fixed table is horizontally arranged, and a turntable is rotatably arranged on the fixed table through a rotating shaft; the vibration cylinder is fixedly arranged on the rotary table, the sample cylinder is arranged in the vibration cylinder, and two opposite sides of the sample cylinder are provided with support shafts which are rotationally connected with the vibration cylinder; the first oscillating mechanism is arranged on the fixed table and used for driving the rotary table to rotate forward and backward alternately; the second vibrates the mechanism and installs in vibrating a section of thick bamboo for drive a sample section of thick bamboo in vibrating a section of thick bamboo swing back and forth.
Preferably, the first oscillating mechanism comprises a toothed ring, a first rack, a second rack, a first driving piece and a second driving piece, the toothed ring is fixedly sleeved outside the turntable, the first rack and the second rack are arranged in parallel, the first rack and the second rack are located on two sides of the toothed ring, the first rack and the second rack are slidably mounted on the fixed table, the first rack and the second rack are meshed with the toothed ring, the first driving piece and the second driving piece are respectively used for driving the first rack and the second rack to move, and the moving directions of the first rack and the second rack are opposite.
Preferably, the first driving member includes a first motor, a first rotating wheel and a first connecting rod, one end of the first connecting rod is hinged to the first rack, the other end of the first connecting rod is eccentrically hinged to the first rotating wheel, and the first rotating wheel is driven by the first motor to rotate.
Preferably, the second driving member includes a second motor, a second rotating wheel and a second connecting rod, one end of the second connecting rod is hinged to the second rack, the other end of the second connecting rod is eccentrically hinged to the second rotating wheel, and the second rotating wheel is driven by the second motor to rotate.
Preferably, the second oscillation mechanism comprises a moving block, a third connecting rod, a fourth connecting rod and an air cylinder, the moving block is slidably mounted on the inner wall of the oscillation cylinder and driven by the air cylinder to move, one end of the third connecting rod and one end of the fourth connecting rod are hinged to the moving block, and the other end of the third connecting rod and the other end of the fourth connecting rod are hinged to the outer wall of the sample cylinder.
Preferably, the outer wall of the sample cylinder is far away from one side of the second oscillating mechanism and is connected with the inner wall of the oscillating cylinder through a plurality of buffering elastic pieces.
Preferably, a sample section of thick bamboo includes the barrel and is connected and carry out the lid sealed to the barrel with the barrel cooperation, and the barrel is inside to be equipped with the test-tube rack, is equipped with the test tube slot that runs through on the test-tube rack.
According to the oscillating and mixing device for cell culture, the first oscillating mechanism drives the rotary table to rotate forward and reversely alternately, the rotary table drives the oscillating cylinder and the sample cylinder to rotate forward and reversely alternately, and meanwhile, the second oscillating mechanism drives the sample cylinder to swing back and forth left and right in the oscillating cylinder, so that culture solution in a culture test tube placed in the sample cylinder is oscillated and mixed uniformly; according to the invention, the first oscillating mechanism and the second oscillating mechanism are arranged, so that the sample cylinder rotates back and forth around the central axis of the oscillating cylinder, and swings back and forth around the central axis of the supporting shaft, thereby improving the oscillating and mixing effect; the invention can adjust the amplitude of the sample cylinder for shaking uniformly by controlling the first shaking mechanism and the second shaking mechanism, and is beneficial to improving the cell culture effect.
Drawings
FIG. 1 is a schematic structural diagram of a shaking and mixing device for cell culture according to the present invention;
fig. 2 is a schematic view of an assembly structure of a sample cylinder and a shaking cylinder in the shaking mixing device for cell culture according to the present invention.
Detailed Description
The invention is further described with reference to the following figures and detailed description.
Referring to fig. 1 and 2, the present invention provides a shaking mixing device for cell culture, which includes a fixed platform 1, a shaking cylinder 2, a sample cylinder, a first shaking mechanism and a second shaking mechanism; wherein:
the fixed station 1 is horizontally arranged, and the turntable 3 is rotatably arranged on the fixed station 1 through a rotating shaft 4.
Vibrate a 2 fixed mounting on carousel 3, a sample section of thick bamboo sets up in vibrating a 2, and the relative both sides of a sample section of thick bamboo all are equipped with and vibrate a back shaft 5 that 2 internal rotations of section of thick bamboo are connected.
The first oscillating mechanism is arranged on the fixed table 1 and used for driving the rotary table 3 to rotate forwards and reversely in an alternating manner.
The second vibrates the mechanism and installs in vibrating a section of thick bamboo 2 for drive sample section of thick bamboo is in vibrating a section of thick bamboo 2 swing back and forth.
In the invention, a first oscillating mechanism drives a rotary table 3 to rotate forward and backward alternately, the rotary table 3 drives an oscillating cylinder 2 and a sample cylinder to rotate forward and backward alternately, and meanwhile, a second oscillating mechanism drives the sample cylinder to swing back and forth left and right in the oscillating cylinder 2, so that a culture solution in a culture test tube placed in the sample cylinder is oscillated and mixed uniformly; according to the invention, the first oscillating mechanism and the second oscillating mechanism are arranged, so that the sample cylinder rotates back and forth around the central axis of the oscillating cylinder 2, and swings back and forth around the central axis of the supporting shaft 5, thereby improving the oscillating and mixing effects; the invention can adjust the amplitude of the sample cylinder for shaking uniformly by controlling the first shaking mechanism and the second shaking mechanism, and is beneficial to improving the cell culture effect.
In this embodiment, the first oscillating mechanism includes a toothed ring 6, a first rack 7, a second rack 8, a first driving member and a second driving member, the toothed ring 6 is fixedly sleeved outside the turntable 3, the first rack 7 and the second rack 8 are arranged in parallel and the first rack 7 and the second rack 8 are located on two sides of the toothed ring 6, the first rack 7 and the second rack 8 are all slidably mounted on the fixed platform 1 and the first rack 7 and the second rack 8 are all engaged with the toothed ring 6, the first driving member and the second driving member are respectively used for driving the first rack 7 and the second rack 8 to move, and the moving directions of the first rack 7 and the second rack 8 are opposite.
Wherein: the first driving part comprises a first motor, a first rotating wheel 9 and a first connecting rod 10, one end of the first connecting rod 10 is hinged with the first rack 7, the other end of the first connecting rod is eccentrically hinged with the first rotating wheel 9, and the first rotating wheel 9 is driven by the first motor to rotate. The second driving part comprises a second motor, a second rotating wheel 11 and a second connecting rod 12, one end of the second connecting rod 12 is hinged with the second rack 8, the other end of the second connecting rod is eccentrically hinged with the second rotating wheel 11, and the second rotating wheel 11 is driven by the second motor to rotate.
In the invention, a first motor and a second motor respectively drive a first rotating wheel 9 and a second rotating wheel 11 at the same speed, the first rotating wheel 9 drives a first rack 7 to move back and forth through a first connecting rod 10, the second rotating wheel 11 drives a second rack 8 to move back and forth through a second connecting rod 12, and the first rack 7 and the second rack 8 drive a toothed ring 6 to rotate forward and backward alternately.
In this embodiment, the second oscillation mechanism includes moving block 13, third connecting rod 14, fourth connecting rod 15 and cylinder 16, and moving block 13 slidable mounting is in oscillating 2 inner walls of section of thick bamboo and is moved by the drive of cylinder 16, and third connecting rod 14, 15 one end of fourth connecting rod all articulate with moving block 13 and third connecting rod 14, the 15 other end of fourth connecting rod all articulate with sample section of thick bamboo outer wall. The cylinder 16 drives the moving block 13 to move up and down and back and forth, and the moving block 13 drives the sample barrel to swing back and forth through the third connecting rod 14 and the fourth connecting rod 15.
In this embodiment, in order to improve a wobbling stability of the sample cylinder, the outer wall of the sample cylinder is kept away from one side of the second oscillating mechanism and is connected with the inner wall of the oscillating cylinder 2 through a plurality of buffering elastic pieces 17, so that the sample cylinder is prevented from being too large in amplitude.
In this embodiment, the sample tube includes barrel 18 and the lid 19 that is connected and seal up barrel 18 with barrel 18 cooperation, and barrel 18 is inside to be equipped with test-tube rack 20, is equipped with the test tube slot 21 that runs through on the test-tube rack 20.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (7)
1. A vibration mixing device for cell culture is characterized by comprising a fixed table, a vibration cylinder, a sample cylinder, a first vibration mechanism and a second vibration mechanism; the fixed table is horizontally arranged, and a turntable is rotatably arranged on the fixed table through a rotating shaft; the vibration cylinder is fixedly arranged on the rotary table, the sample cylinder is arranged in the vibration cylinder, and two opposite sides of the sample cylinder are provided with support shafts which are rotationally connected with the vibration cylinder; the first oscillating mechanism is arranged on the fixed table and used for driving the rotary table to rotate forward and backward alternately; the second vibrates the mechanism and installs in vibrating a section of thick bamboo for drive a sample section of thick bamboo in vibrating a section of thick bamboo swing back and forth.
2. The cell culture shaking mixing device of claim 1, wherein the first shaking mechanism comprises a toothed ring, a first rack, a second rack, a first driving member and a second driving member, the toothed ring is fixedly sleeved outside the turntable, the first rack and the second rack are arranged in parallel and are located on two sides of the toothed ring, the first rack and the second rack are slidably mounted on the fixed platform and are engaged with the toothed ring, the first driving member and the second driving member are respectively used for driving the first rack and the second rack to move, and the moving directions of the first rack and the second rack are opposite.
3. The shaking mixing device for cell culture according to claim 2, wherein the first driving member comprises a first motor, a first rotating wheel and a first connecting rod, one end of the first connecting rod is hinged to the first rack, the other end of the first connecting rod is eccentrically hinged to the first rotating wheel, and the first rotating wheel is driven by the first motor to rotate.
4. The shaking mixing device for cell culture according to claim 2, wherein the second driving member comprises a second motor, a second rotating wheel and a second connecting rod, one end of the second connecting rod is hinged to the second rack and the other end of the second connecting rod is eccentrically hinged to the second rotating wheel, and the second rotating wheel is driven by the second motor to rotate.
5. The shaking mixing device for cell culture according to any one of claims 1 to 4, wherein the second shaking mechanism comprises a moving block, a third connecting rod, a fourth connecting rod and a cylinder, the moving block is slidably mounted on the inner wall of the shaking cylinder and driven by the cylinder to move, one end of each of the third connecting rod and the fourth connecting rod is hinged with the moving block, and the other end of each of the third connecting rod and the fourth connecting rod is hinged with the outer wall of the sample cylinder.
6. The shaking mixing device for cell culture according to claim 5, wherein the outer wall of the sample cylinder is further connected to the inner wall of the shaking cylinder through a plurality of buffering elastic members at a side thereof away from the second shaking mechanism.
7. The shaking mixing device for cell culture according to any one of claims 1 to 4, wherein the sample cartridge comprises a cartridge body and a cover body which is connected with the cartridge body in a matching way and seals the cartridge body, a test tube rack is arranged inside the cartridge body, and a test tube slot is arranged on the test tube rack.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010353804.5A CN111500450A (en) | 2020-04-29 | 2020-04-29 | Cell culture is with shaking mixing arrangement |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010353804.5A CN111500450A (en) | 2020-04-29 | 2020-04-29 | Cell culture is with shaking mixing arrangement |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111500450A true CN111500450A (en) | 2020-08-07 |
Family
ID=71866605
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010353804.5A Withdrawn CN111500450A (en) | 2020-04-29 | 2020-04-29 | Cell culture is with shaking mixing arrangement |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111500450A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112694973A (en) * | 2020-12-11 | 2021-04-23 | 青岛市中心血站 | Freezing mixing sieving mechanism of nucleic acid testing sample |
-
2020
- 2020-04-29 CN CN202010353804.5A patent/CN111500450A/en not_active Withdrawn
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112694973A (en) * | 2020-12-11 | 2021-04-23 | 青岛市中心血站 | Freezing mixing sieving mechanism of nucleic acid testing sample |
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| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20200807 |
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| WW01 | Invention patent application withdrawn after publication |