CN112694973A - Freezing mixing sieving mechanism of nucleic acid testing sample - Google Patents
Freezing mixing sieving mechanism of nucleic acid testing sample Download PDFInfo
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- CN112694973A CN112694973A CN202011453221.6A CN202011453221A CN112694973A CN 112694973 A CN112694973 A CN 112694973A CN 202011453221 A CN202011453221 A CN 202011453221A CN 112694973 A CN112694973 A CN 112694973A
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- 238000002156 mixing Methods 0.000 title claims abstract description 84
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 54
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 54
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 54
- 230000007246 mechanism Effects 0.000 title claims abstract description 47
- 238000007710 freezing Methods 0.000 title claims abstract description 23
- 230000008014 freezing Effects 0.000 title claims abstract description 23
- 238000012360 testing method Methods 0.000 title claims abstract description 11
- 238000007873 sieving Methods 0.000 title claims abstract description 5
- 238000001514 detection method Methods 0.000 claims abstract description 53
- 238000010438 heat treatment Methods 0.000 claims abstract description 15
- 238000012216 screening Methods 0.000 claims abstract description 14
- 238000012806 monitoring device Methods 0.000 claims abstract description 9
- 230000001678 irradiating effect Effects 0.000 claims abstract description 6
- 230000033001 locomotion Effects 0.000 claims description 14
- 238000012546 transfer Methods 0.000 claims description 8
- 238000005485 electric heating Methods 0.000 claims description 5
- 230000003287 optical effect Effects 0.000 claims description 4
- 230000005540 biological transmission Effects 0.000 claims description 3
- 239000000523 sample Substances 0.000 abstract description 55
- 238000010257 thawing Methods 0.000 abstract description 9
- 238000002834 transmittance Methods 0.000 abstract description 9
- 230000000694 effects Effects 0.000 abstract description 8
- 239000012520 frozen sample Substances 0.000 abstract description 7
- 238000003780 insertion Methods 0.000 description 12
- 230000037431 insertion Effects 0.000 description 12
- 238000000034 method Methods 0.000 description 6
- 239000008280 blood Substances 0.000 description 4
- 210000004369 blood Anatomy 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000002844 melting Methods 0.000 description 2
- 239000013062 quality control Sample Substances 0.000 description 2
- 239000002253 acid Substances 0.000 description 1
- 239000000956 alloy Substances 0.000 description 1
- 229910045601 alloy Inorganic materials 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000005674 electromagnetic induction Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000011330 nucleic acid test Methods 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F2101/00—Mixing characterised by the nature of the mixed materials or by the application field
- B01F2101/23—Mixing of laboratory samples e.g. in preparation of analysing or testing properties of materials
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Abstract
The invention discloses a freezing, mixing and screening device for a nucleic acid detection sample, and relates to the technical field of experimental instruments. Freezing mixing sieving mechanism of nucleic acid testing sample, including the box and with box articulated lid, include in the box: a vibrate mixing device for freezing sample mixing for drive vibrates mixing device pivoted motor I, locate heating device and temperature monitoring device in the box. According to the invention, the oscillating mixing device is arranged and matched with the horizontal rotation of the motor I, so that the frozen sample is fully mixed in 2 mixing directions, and the mixing effect is good, simple and efficient; by arranging the light direct irradiating device and the light receiving device, the thawing and mixing conditions of the sample can be reflected in real time through light transmittance detection; meanwhile, the invention can be automatically fed back and controlled according to the sample mixing condition, and the degree of automation is high.
Description
Technical Field
The invention relates to the technical field of experimental instruments, in particular to a freezing, uniformly mixing and screening device for a nucleic acid detection sample.
Background
Blood donation is a daunting behavior without private contribution, and rescuing and strengthening injuries, and is also an embodiment of love contribution. According to the national regulations, nucleic acid detection is required for blood collected from blood donors to ensure the safety of blood quality.
For the sample for nucleic acid detection, the detection should be completed within 72 hours after sampling, the sample which can not be detected within the above time limit due to special conditions should be frozen below-18 ℃, and when the frozen sample is re-detected, the re-melting should be performed at 18-25 ℃.
At present, the method for detecting the nucleic acid sample after freezing is as follows: unfreezing a nucleic acid detection sample at 18-25 ℃, generally completing unfreezing within 20min, and after unfreezing is completed, shaking and shaking the nucleic acid detection sample by a worker until the sample is uniformly mixed; the operation is complex, time and labor are wasted, and the re-melting efficiency of the sample is influenced.
How to solve the technical problems is a technical problem to be solved in the technical field of the existing experimental instruments.
Disclosure of Invention
In view of the above technical problems, the embodiments of the present invention provide a device for freezing, mixing, and screening a nucleic acid detection sample, so as to solve the problems in the background art.
The invention provides the following technical scheme: the utility model provides a freezing mixing sieving mechanism of nucleic acid testing sample, includes the box and with box articulated lid, include in the box: a vibrate mixing device for freezing sample mixing for drive vibrates mixing device pivoted motor I, locate heating device and temperature monitoring device in the box.
Preferably, the oscillating and mixing device comprises a mixing box, wherein a sliding chute I and a sliding chute II which are arranged in parallel are respectively arranged on two side walls of the mixing box, a support I and a support II are respectively connected to the sliding chute I and the sliding chute II in a sliding manner, a plurality of rows of jacks which are arranged at intervals are arranged on the support I, and slots corresponding to the number and arrangement mode of the jacks are arranged on the support II;
support I and II one sides of support are connected reciprocal connecting rod actuating mechanism I and reciprocal connecting rod actuating mechanism II respectively, reciprocal connecting rod actuating mechanism I is the same with reciprocal connecting rod actuating mechanism II structure, reciprocal connecting rod actuating mechanism I is used for driving support I and is reciprocal linear motion on spout I, reciprocal connecting rod actuating mechanism II is used for driving support II and is reciprocal linear motion on spout II, and support I and II opposite directions of motion of support.
Preferably, the reciprocating link driving mechanism i includes: the device comprises a driving device, a rotating shaft I connected with the output end of the driving device, a connecting rod I fixedly connected with the rotating shaft I, a rotating shaft II in transfer connection with the connecting rod I, a connecting rod II in transfer connection with the rotating shaft II and a connecting plate fixedly connected with the connecting rod II; and the reciprocating connecting rod driving mechanism I is fixedly connected with the bracket I through a connecting plate.
Preferably, the driving device comprises a motor II, and the motor II is in transmission connection with the rotating shaft I through a gear.
Preferably, the method further comprises the following steps: the light receiving device is arranged on one side wall of the blending box, and the light direct irradiating device is arranged on the other side wall of the blending box and is opposite to the light receiving device.
Preferably, the light receiving device comprises a flat plate fixedly connected to the side wall of the blending box and light sensors arranged on the flat plate at intervals; the number and the positions of the optical sensors correspond to the number and the positions of the jack rows.
Preferably, the light receiving device is positioned on the side wall of the blending box between the sliding groove I and the sliding groove II.
Preferably, the side wall of the box body is provided with a cavity, a heating device is arranged in the cavity, and the heating device is an electric heating element.
Preferably, the inner side wall of the box body is provided with a plurality of heat transfer holes.
Preferably, the temperature monitoring device is a temperature sensor.
The freezing, mixing and screening device for the nucleic acid detection sample provided by the embodiment of the invention has the following beneficial effects:
1. according to the invention, the oscillating mixing device is arranged and matched with the horizontal rotation of the motor I, so that the frozen sample is fully mixed in 2 mixing directions, and the mixing effect is good, simple and efficient;
2. through the arrangement of the reciprocating link mechanism I and the reciprocating link driving mechanism II, the reciprocating link mechanism I and the reciprocating link driving mechanism II respectively drive the support I and the support II to move in opposite directions, so that the nucleic acid detection sample is vibrated and mixed uniformly, the mixing effect is better, the movement mode of the sample also accords with the experimental habit of shaking the sample by vibration of workers, and the practical popularization and application are facilitated;
3. by arranging the light direct irradiating device and the light receiving device, the thawing and mixing conditions of the sample can be reflected in real time through light transmittance detection; meanwhile, the invention can be automatically fed back and controlled according to the sample mixing condition, and the degree of automation is high.
Drawings
FIG. 1 is a perspective view of a freezing, mixing and screening apparatus for nucleic acid detection samples according to the present invention;
FIG. 2 is a schematic structural diagram of a freezing, mixing and screening apparatus for nucleic acid detection samples according to the present invention;
FIG. 3 is a schematic structural view of a vibrating and mixing device according to the present invention;
FIG. 4 is a schematic view of a part of the oscillating mixer of the present invention;
FIG. 5 is a schematic cross-sectional view of a freezing, mixing and screening apparatus for nucleic acid detection samples according to the present invention;
FIG. 6 is a schematic view of a driving device according to the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments obtained by a person skilled in the art based on the embodiments of the present invention without any creative work belong to the protection scope of the present invention.
Example 1, see fig. 1-5.
A freezing, mixing and screening device for nucleic acid detection samples comprises a box body 110 and a cover body 120 hinged with the box body 110, wherein the cover body 120 can be opened and closed relative to the box body 110;
the device comprises a box body 110, a vibration blending device 200 for blending frozen samples, a rotating disc 140, a motor I130, a shell of the motor I130, a heating device and a temperature monitoring device, wherein the vibration blending device 200 for blending frozen samples is arranged in the box body 110, the bottom of the vibration blending device 200 is fixedly arranged on the rotating disc 140, the rotating disc 140 is fixedly connected with an output shaft of the motor I130, the motor I130 is used for driving the vibration blending device 200 to rotate, the shell of the motor I130 is fixedly arranged on; preferably, the temperature monitoring device is a temperature sensor 250.
In the present embodiment, the frozen sample refers to a nucleic acid test sample that is frozen and needs to be thawed.
The invention heats the nucleic acid detection sample stored in the box body 110 by the heating device, and performs feedback adjustment on the heating temperature by the temperature monitoring device, so that the temperature in the box body 110 is controlled at 18-25 ℃ to meet the temperature requirement for unfreezing the nucleic acid detection sample.
According to the invention, the nucleic acid detection sample is uniformly mixed in a vibrating manner through the vibrating and uniformly mixing device 200, meanwhile, the motor I130 horizontally rotates at a constant speed, so that the nucleic acid detection sample is uniformly mixed in the horizontal direction, and the vibrating and uniformly mixing device 200 is matched with the motor I130 to horizontally rotate at a constant speed, so that the nucleic acid detection sample is fully and uniformly mixed, and the sample thawing and thawing efficiency is improved.
Example 2, see fig. 2, 3, 4 and 5.
The invention also discloses the following technical scheme: the oscillating and mixing device 200 comprises a mixing box 290, wherein two side walls of the mixing box 290 are respectively provided with a sliding groove I221 and a sliding groove II 222 which are arranged in parallel, the sliding groove I221 and the sliding groove II 222 are respectively connected with a bracket I230 and a bracket II 240 in a sliding manner, the bracket I230 is provided with a plurality of rows of insertion holes 231 which are arranged at intervals, and the bracket II 240 is provided with insertion grooves 241 corresponding to the number and arrangement mode of the insertion holes 231;
it should be noted that, in the embodiment of the present invention, 5 rows of the insertion holes 231 and the insertion slots 241 are provided, and each row is provided with 4 insertion holes; in use, the nucleic acid detecting sample is sequentially inserted into the insertion hole 231 and the insertion groove 241, so that the nucleic acid detecting sample can be conveniently positioned.
Preferably, the insertion hole 231 and the insertion groove 241 are coaxially arranged, and an annular buffer rubber ring is arranged in the insertion hole 231, and the annular buffer rubber ring is used for fastening the nucleic acid detection sample and preventing the nucleic acid detection sample from shaking left and right or front and back too much in the horizontal direction, so that a buffer protection effect is achieved; the slot 241 is semicircular and has a hole diameter slightly larger than the bottom of the nucleic acid detection sample, so that the bottom of the nucleic acid detection sample has a free moving space in the slot 241.
One sides of the bracket I230 and the bracket II 240 are respectively connected with a reciprocating connecting rod driving mechanism I210 and a reciprocating connecting rod driving mechanism II 280, and the reciprocating connecting rod driving mechanism I210 and the reciprocating connecting rod driving mechanism II 280 have the same structure; however, the specific size requirements of the reciprocating link driving mechanism I210 and the reciprocating link driving mechanism II 280 are set according to the specific size of the bracket I230 and the bracket II 240.
The reciprocating link mechanism I210 is used for driving the bracket I230 to do reciprocating linear motion on the sliding chute I221, the reciprocating link driving mechanism II 280 is used for driving the bracket II 240 to do reciprocating linear motion on the sliding chute II 222, and the moving directions of the bracket I230 and the bracket II 240 are opposite.
In use, the reciprocating link mechanism I210 and the reciprocating link driving mechanism II 280 respectively drive the bracket I230 and the bracket II 240 to move on the sliding chute in opposite directions, so that the samples are uniformly mixed; and the motion mode of the sample also accords with the experiment habit that the worker shakes and shakes the sample, and the maximum accords with the conventional operation mode of the worker, relatively speaking, under the prerequisite that does not change the experiment operation mode, makes the experimental flow standardized more easily, also can reduce experimental error, improves the experiment precision.
Example 3, see fig. 5 and 6.
The invention also discloses the following technical scheme: the reciprocating link driving mechanism i 210 includes: the device comprises a driving device 211, a rotating shaft I212 connected with the output end of the driving device 211, a connecting rod I213 fixedly connected with the end part of the rotating shaft I212, a rotating shaft II 214 rotatably connected with one end of the connecting rod I213, a connecting rod II 215 rotatably connected with the other end of the rotating shaft II 214, and a connecting plate 216 fixedly connected with the other end of the connecting rod II 215; the reciprocating link driving mechanism I210 is fixedly connected with the bracket I230 through a connecting plate 216.
When the device is used, the driving device 211 drives the rotating shaft I212 to rotate, the rotating shaft I212 performs horizontal uniform-speed circular motion to drive the rotating shaft II 214 and the connecting rod II 215 to rotate, and the support I230 achieves reciprocating linear motion under the left and right limiting positions of the sliding groove I221.
The reciprocating link driving mechanism II 280 is identical to the reciprocating link driving mechanism I210 in structure, and the reciprocating link driving mechanism II 280 can also drive the bracket II 240 to do reciprocating linear motion on the sliding chute II 222.
The structure of the compound connecting rod driving mechanism II 280 is as follows: it includes: the device comprises a driving device, a rotating shaft I connected with the output end of the driving device, a connecting rod I fixedly connected with the end part of the rotating shaft I, a rotating shaft II rotatably connected with one end of the connecting rod I, a connecting rod II rotatably connected with the other end of the rotating shaft II, and a connecting plate fixedly connected with the other end of the connecting rod II; reciprocating connecting rod actuating mechanism I passes through connecting plate and I fixed connection of support.
Preferably, the driving device 211 comprises a motor ii 211a, and the motor ii 211a is in gear transmission connection with the rotating shaft i 212.
Preferably, the output end of the motor ii 211a is provided with a bevel gear i 211b, the bevel gear i 211b is engaged with a bevel gear ii 211c, the bevel gear ii 211c is fixedly connected to a rotating shaft iii 211d, the rotating shaft iii 211d is rotatably arranged on a box body of the driving device 211, the other side of the bevel gear ii 211c is engaged with a bevel gear iii 211f, the bevel gear iii 211f is fixedly connected to a rotating shaft iv 211e, the rotating shaft iv 211e is rotatably arranged on the box body of the driving device 211, the rotating shaft iv 211e is further provided with a gear i 211g, the gear i 211g is engaged with a gear ii 211h, and the gear ii 211h is fixedly connected to the rotating shaft i 212.
Preferably, a spring 260 arranged on one side opposite to the reciprocating link driving mechanism I210 is arranged on the bracket I230, and the other end of the spring 260 is fixedly connected with the side wall of the box body 110; a spring 260 arranged on one side opposite to the reciprocating connecting rod driving mechanism II 280 is arranged on the bracket II 240, and the other end of the spring 260 is fixedly connected with the side wall of the box body 110; through setting up spring 260, can play the cushioning effect, prevent that I230 of support or II 240 of support from taking place to exert oneself uneven phenomenon under the effect of reciprocal connecting rod actuating mechanism drive, avoid arousing support vibrations.
Example 4, see fig. 4.
In order to ensure the accuracy and precision of the detection result of the nucleic acid sample, for the nucleic acid detection samples of the same batch, the reagents, equipment and operation steps in the experimental detection process are required to be consistent in experimental conditions, so that the effectiveness of the experimental result is prevented from being influenced; meanwhile, an external quality control sample is required to be arranged, and all the nucleic acid detection samples and the experimental operation of the external quality control sample are required to be consistent.
In the same batch of nucleic acid detection samples, individual differences always occur, and the thawing and mixing process takes longer time; however, in order to ensure the consistency of the experimental conditions, the thawing and mixing processes are required to be performed for the same time for the same batch of samples.
It should be noted that, in the thawing and blending process, in order to maintain the constant temperature condition of 18-25 ℃ inside the box body 110, the invention needs to be performed in a sealed state, that is: the cover 120 is kept closed with the case 110.
However, lid 120 and box 110 are in the closed condition, and operating personnel can't accurately know whether the sample unfreezes completely and the mixing, if open lid 120 repeatedly and observe, can influence the inside constant temperature condition of box 110, simultaneously, also influences work efficiency, needs row's special person on duty, influences work efficiency.
In order to solve the technical problem, the inventor makes the following improvements:
the invention also comprises a light receiving device arranged on one side wall of the blending box 290 and a light direct-irradiating device arranged on the other side wall of the blending box 290 and arranged opposite to the light receiving device.
Preferably, the light receiving device comprises a flat plate 300 fixedly connected to the side wall of the blending box 290, and light sensors 310 arranged on the flat plate 300 at intervals; the number and the positions of the light sensors 310 correspond to the number and the positions of the rows of the jacks 231;
the number of the optical sensors 310 is the same as the number of the arrangement of the jacks 231, in the embodiment of the present invention, 5 rows of the jacks 231 are provided; therefore, 5 photosensors 310 are also provided, and the photosensors 310 are located on the same horizontal line as each row of the insertion holes 231.
Preferably, the light directing device is a point light source, preferably an LED lamp, and the light emitted from the LED lamp can direct the nucleic acid detection samples fixed on the support, then the light passes through the nucleic acid detection samples in each row, and finally reaches the light sensor 310, and the light sensor 310 receives the light from the direct light passing through the nucleic acid detection samples in a single row.
The invention further comprises a controller, the controller is a single chip microcomputer or other microprocessors, the motor I130, the motor II 211a, the heating device, the temperature monitoring device, the light receiving device and the light direct-emitting device are all electrically connected with the controller, and the automatic control of the invention is realized by arranging the controller.
Preferably, the present invention further comprises an AD conversion module, which converts the analog voltage signal generated by the light sensor 310 into a digital signal and transmits the digital signal to the microprocessor, and the microprocessor calculates the light intensity of the direct light according to the digital signal, and further calculates the light transmittance of each row of nucleic acid detection samples by combining the light intensity of the light source.
The detection principle of the invention is as follows: in the embodiment of the invention, 5 rows of nucleic acid detection samples are arranged, a point light source LED lamp is arranged on the front side of each row of nucleic acid detection samples, the point light source LED lamp is used for directly irradiating the nucleic acid detection samples in each row, then the optical sensor 310 on the rear side of each row of nucleic acid detection samples detects light rays penetrating through 5 nucleic acid detection samples in each row, and whether the samples are uniformly mixed or not (the light transmittance is different before and after the samples are uniformly mixed) is judged by calculating the light transmittance; before test detection, a threshold value is required to be set, the threshold value is higher than or lower than the threshold value, a certain sample in each row can definitely have the condition of not being mixed uniformly, the mixing is required to be continued, and for the setting of the threshold value, the average value is optimally set by collecting all light transmittance results of the samples which are mixed uniformly for many times.
It should be noted that, in order to determine the accuracy of the sample mixing result, the detection of the direct light is generally performed when the nucleic acid detection sample is in a vertical state (i.e., the acid detection sample is perpendicular to the horizontal plane, and at this time, the holder i 230 and the holder ii 240 both move to the horizontal position), and the light beam emission direction of the LED is perpendicular to the central axis of the sample.
For the thawing and mixing operation time in the invention, the light transmittance calculated by the light intensity of the direct light received by the light sensor 310 can be fed back, and if the light transmittance is all in the threshold range, the controller gives instructions to stop working to each functional template, namely the working is finished; meanwhile, the voice reminding module can be arranged, so that the voice reminding module can remind a worker after detecting that the samples are completely mixed, and the worker can prepare in time to carry out the next step of work.
Preferably, the light receiving device is positioned on the side wall of the blending box 290 between the chute I221 and the chute II 222, and the position of the light receiving device can facilitate the detection of a nucleic acid detection sample;
preferably, light receiver is located the mixing box 290 lateral wall that is close to I221 one side of spout, because the nucleic acid testing sample is generally placed in transparent test tube, and the sample that awaits measuring gets the middle and upper segment part of nucleic acid testing sample more, so need focus on the middle and upper segment mixing condition of nucleic acid testing sample, the setting of this scheme position can the near light intensity of direct light of upper portion in the test tube of optimal collection.
Preferably, a cavity 151 is formed in the side wall of the box body 110, a heating device is arranged in the cavity 151, and the heating device is an electric heating element; the electric heating element may be: electrothermal alloy, electrothermal material, microwave heating device, electromagnetic induction heating device, electrothermal wire, electrothermal plate, electrothermal belt, electrothermal cable, electrothermal disk, electrothermal couple, electric heating ring, electrothermal rod and electric tracing belt.
Preferably, the inner sidewall of the box 110 is provided with a plurality of heat transfer holes 152, and the heat generated in the cavity 151 of the sidewall of the box 110 can be transferred into the box 110 by the heat transfer holes 152.
The freezing, mixing and screening device for the nucleic acid detection sample provided by the embodiment of the invention has the following beneficial effects:
according to the invention, the oscillating mixing device is arranged and matched with the horizontal rotation of the motor I, so that the frozen sample is fully mixed in 2 mixing directions, and the mixing effect is good, simple and efficient; the reciprocating link mechanism I and the reciprocating link driving mechanism II are arranged to respectively drive the bracket I and the bracket II to move in opposite directions, so that the uniform mixing effect is better, the movement mode of the sample also accords with the experimental habit of shaking and shaking the sample by workers, and the practical popularization and application are facilitated; by arranging the light direct irradiating device and the light receiving device, the thawing and mixing conditions of the sample can be reflected in real time through light transmittance detection; meanwhile, the invention can be automatically fed back and controlled according to the sample mixing condition, and the degree of automation is high.
The above-described embodiments of the apparatus are merely illustrative, and the units described as separate parts may or may not be physically separate, and parts displayed as units may or may not be physical units, may be located in one place, or may be distributed on a plurality of network units. Some or all of the modules may be selected according to actual needs to achieve the purpose of the solution of the present embodiment.
In the present invention, unless otherwise expressly specified or limited, the terms "mounted," "disposed," "connected," "secured," "screwed" and the like are to be construed broadly, e.g., as meaning fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; the terms may be directly connected or indirectly connected through an intermediate, and may be communication between two elements or interaction relationship between two elements, unless otherwise specifically limited, and the specific meaning of the terms in the present invention will be understood by those skilled in the art according to specific situations.
It should be understood that the technical solutions and concepts of the present invention may be equally replaced or changed by those skilled in the art, and all such changes or substitutions should fall within the protection scope of the appended claims.
Claims (10)
1. The utility model provides a freezing mixing sieving mechanism of nucleic acid testing sample, includes the box and with box articulated lid, its characterized in that, include in the box: a vibrate mixing device for freezing sample mixing for drive vibrates mixing device pivoted motor I, locate heating device and temperature monitoring device in the box.
2. The device for freezing, mixing and screening the nucleic acid detection sample according to claim 1, wherein the shaking and mixing device comprises a mixing box, two side walls of the mixing box are respectively provided with a sliding chute I and a sliding chute II which are arranged in parallel, the sliding chute I and the sliding chute II are respectively connected with a bracket I and a bracket II in a sliding manner, the bracket I is provided with a plurality of rows of jacks which are arranged at intervals, and the bracket II is provided with slots corresponding to the number and arrangement mode of the jacks;
support I and II one sides of support are connected reciprocal connecting rod actuating mechanism I and reciprocal connecting rod actuating mechanism II respectively, reciprocal connecting rod actuating mechanism I is the same with reciprocal connecting rod actuating mechanism II structure, reciprocal connecting rod actuating mechanism I is used for driving support I and is reciprocal linear motion on spout I, reciprocal connecting rod actuating mechanism II is used for driving support II and is reciprocal linear motion on spout II, and support I and II opposite directions of motion of support.
3. The apparatus for freezing, mixing and screening nucleic acid detection samples according to claim 2, wherein the reciprocating link driving mechanism I comprises: the device comprises a driving device, a rotating shaft I connected with the output end of the driving device, a connecting rod I fixedly connected with the rotating shaft I, a rotating shaft II in transfer connection with the connecting rod I, a connecting rod II in transfer connection with the rotating shaft II and a connecting plate fixedly connected with the connecting rod II; and the reciprocating connecting rod driving mechanism I is fixedly connected with the bracket I through a connecting plate.
4. The device for freezing, uniformly mixing and screening the nucleic acid detection sample according to claim 3, wherein the driving device comprises a motor II, and the motor II is in transmission connection with the rotating shaft I through a gear.
5. The apparatus of claim 2, further comprising: the light receiving device is arranged on one side wall of the blending box, and the light direct irradiating device is arranged on the other side wall of the blending box and is opposite to the light receiving device.
6. The nucleic acid detection sample freezing and mixing screening device of claim 5, wherein the light receiving device comprises a flat plate fixedly connected to the side wall of the mixing box, and light sensors arranged on the flat plate at intervals; the number and the positions of the optical sensors correspond to the number and the positions of the jack rows.
7. The apparatus according to claim 5 or 6, wherein the light receiving device is located on the side wall of the mixing box between the chute I and the chute II.
8. The device for freezing, uniformly mixing and screening the nucleic acid detection sample according to claim 1, wherein a cavity is formed in the side wall of the box body, a heating device is arranged in the cavity, and the heating device is an electric heating element.
9. The apparatus of claim 8, wherein the inner sidewall of the housing has a plurality of heat transfer holes.
10. The apparatus according to claim 1, wherein the temperature monitoring device is a temperature sensor.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011453221.6A CN112694973A (en) | 2020-12-11 | 2020-12-11 | Freezing mixing sieving mechanism of nucleic acid testing sample |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011453221.6A CN112694973A (en) | 2020-12-11 | 2020-12-11 | Freezing mixing sieving mechanism of nucleic acid testing sample |
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Citations (4)
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|---|---|---|---|---|
| US20120329058A1 (en) * | 2009-12-30 | 2012-12-27 | Huadong Researc Institute For Medicine And Biotechnics | Method and device for fast detecting nucleic acid |
| CN203044299U (en) * | 2013-01-28 | 2013-07-10 | 苏州培英实验设备有限公司 | High-capacity double-layer reciprocating oscillator |
| CN209985335U (en) * | 2019-11-13 | 2020-01-24 | 北京中医药大学 | Mechanical oscillation mixing device |
| CN111500450A (en) * | 2020-04-29 | 2020-08-07 | 杨慧慧 | Cell culture is with shaking mixing arrangement |
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2020
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120329058A1 (en) * | 2009-12-30 | 2012-12-27 | Huadong Researc Institute For Medicine And Biotechnics | Method and device for fast detecting nucleic acid |
| CN203044299U (en) * | 2013-01-28 | 2013-07-10 | 苏州培英实验设备有限公司 | High-capacity double-layer reciprocating oscillator |
| CN209985335U (en) * | 2019-11-13 | 2020-01-24 | 北京中医药大学 | Mechanical oscillation mixing device |
| CN111500450A (en) * | 2020-04-29 | 2020-08-07 | 杨慧慧 | Cell culture is with shaking mixing arrangement |
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| 玻璃钢编辑部: "《化工仪器仪表手册》", 上海科学技术出版社, pages: 186 - 187 * |
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Application publication date: 20210423 |