CN111500327A - Biological detergent for gasoline - Google Patents

Abstract

The invention relates to a gasoline biological detergent, which is characterized by containing Rhodococcus erythropolis T7-3 and fermentation supernatant of Haichilus halophila L HOD-1. the application method of the gasoline biological detergent comprises the steps of mixing the gasoline biological detergent with gasoline and 1% sodium chloride solution according to the mass ratio of 1: 100: 100, carrying out vortex stirring reaction at 50rpm for 36 hours, standing for more than 2 hours after the reaction is finished, waiting for oil-water layering, and collecting an oil layer by using a separating funnel, wherein the oil layer is modified clean gasoline.

Description

Biological detergent for gasoline

Technical Field

The invention relates to application of microorganisms in the field of clean energy, in particular to a gasoline biological detergent and application thereof.

Background

Increasingly stringent energy saving and environmental requirements are always the goal and motivation for the continued development of the automotive industry. Since the 80 s in the 20 th century, automobile engines in developed countries abroad widely use electrically controlled hole type fuel injectors to replace original carburetors, so that the emission of harmful substances is reduced by about Mao percent, and the oil consumption is reduced by about 5 percent. However, the working conditions of the electronic fuel injection engine are more severe, and deposits such as paint films, carbon deposits and the like are easily formed on the gasoline at the positions of an engine nozzle, an air inlet valve, a combustion chamber and the like, so that the combustion process is deteriorated, and the emission and the oil consumption are increased. Gasoline detergents are very effective in controlling engine deposits and are therefore widely used abroad.

Along with the development of the automobile industry in China, the possession quantity of an electronic fuel injection vehicle increases year by year, the quality of finished oil in China at present has a larger difference compared with that of the gasoline in China, the proportion of catalytic cracking components in the gasoline is larger, the content of unsaturated hydrocarbon is high, the tendency of forming sediments in an engine is more serious, and the addition of an effective gasoline detergent is particularly important, which is also a necessary trend of the clean development of the gasoline for the China.

China gasoline detergent has the second generation and the third generation products at present, the fourth generation detergent belongs to the exploration stage, and no microbial preparation aiming at the gasoline cleaning and dispersing effect exists at present.

Disclosure of Invention

Aiming at the defects of the gasoline detergent in the market at present, the invention aims to research a gasoline biological detergent and application thereof, the gasoline biological detergent has good cleaning effect, is environment-friendly and does not harm the environment, can effectively reduce the quality of deposits in a combustion chamber, has lower oil consumption rate, and can also increase the lubricity.

The invention is realized by the following technical scheme:

rhodococcus erythropolis T7-3 strain used in the invention is deposited in China general microbiological culture Collection center (CGMCC) on 5, 14 days 2012, and the preservation number is CGMCC No.6104, which is disclosed by Chinese patent.

The halophyte L HOD-1 used in the invention has a preservation unit of common microorganism center of China Committee for culture Collection of microorganisms, the address of the preservation date of the culture preservation center of Beijing institute of microorganisms, Beijing Zhongzhong academy of sciences, Beijing, is 2012-10-29 days, and the preservation number is CGMCC No.6715, which is disclosed by Chinese patent.

The Rhodococcus erythropolis is known to degrade crude oil, and the halophyte L HOD-1 is known to produce a surfactant to facilitate dispersion, solubilization, and emulsification of petroleum contaminants.

The invention aims to biologically modify gasoline so as to effectively reduce the quality of deposits in a combustion chamber and increase the lubricity of the gasoline. We do not need Rhodococcus erythropolis T7-3 to degrade crude oil, but need it to modify crude oil.

The group of the invention finds that the Rhodococcus erythropolis T7-3 is subjected to induction culture, and the fermentation supernatant of the halophyte L HOD-1 is added into the final microbial inoculum, so that the Rhodococcus erythropolis T7-3 can be effectively induced to modify rather than degrade crude oil, the quality of deposits in the modified gasoline combustion chamber is greatly reduced, the oil consumption rate is lower, and a certain lubricating property is also improved.

The gasoline biological cleaning agent is characterized in that:

contains Rhodococcus erythropolis T7-3 and Haematococcus halophycus L HOD-1 fermentation supernatant.

The preparation method of the gasoline biological detergent comprises the following steps:

(1) activated Rhodococcus erythropolis T7-3:

inoculating Rhodococcus erythropolis T7-3 strain in glycerol freezing tube, selecting 3 rings, inoculating into 80m L L B shake flask, shake culturing at 65 deg.C and 120rpm for 24h, and preparing into 10⁸CFU/ml bacterial liquid;

the L B culture medium formula is as follows:

according to the weight percentage, the peptone is 1 percent, the yeast powder is 0.5 percent, the NaCl is 1 percent, and the balance is purified water, and the pH value is 7.0;

(2) preparing a fermentation supernatant of marine salina bacteria L HOD-1:

culturing frozen halophyte L HOD-1 in L B culture medium additionally containing 5 g/L of sucrose for 2 days, supplementing sucrose every day during the culture, keeping the sucrose concentration of the culture medium at 4-5 g/L, centrifuging the culture at 5000r/min for 10min after the culture is finished, and collecting supernatant;

(3) and (3) induction culture:

transferring the strain into an induction culture medium, wherein the inoculation amount is 10%, and performing shaking culture for 24-36 h;

the induction culture medium:

1% of liquid wax, 8% of fermentation supernatant of Haichilus halophila L HOD-1, 1.5% of yeast powder, 1% of sucrose, 0.5% of trehalose, 0.1% of sodium dihydrogen phosphate and 0.1% of potassium dihydrogen phosphate, wherein the pH value is 7.2;

(4) after induction culture, carrying out centrifugal treatment, and uniformly stirring to obtain thalli;

(5) preparing a gasoline biological detergent:

and (3) adding 1 time of the thalli obtained in the step (4) by weight into 1 time of the tween 80 by weight, 0.1 time of the fermentation supernatant of the marine salina L HOD-1 by weight, 5 times of oleic acid by weight, 1 time of trehalose by weight and 0.5 time of magnesium chloride by weight, and uniformly mixing to obtain thalli mixed oily liquid, namely the gasoline biological detergent.

The product can be used by shaking before use, and is optimally stored by refrigeration.

The use method of the gasoline biological detergent comprises the following steps:

mixing the gasoline biological detergent with gasoline and a 1% sodium chloride solution according to the mass ratio of 1: 100: mixing at the ratio of 100, and stirring in a vortex at 50rpm for 36 hours; after the reaction is finished, standing for more than 2 hours for oil-water stratification, and then collecting an oil layer by using a separating funnel, wherein the oil product is the modified clean gasoline.

Oleic acid is mainly from nature and exists in animal and vegetable oil in the form of glyceride. And (3) saponifying and acidifying the grease with high oleic acid content to obtain oleic acid. The invention is beneficial to protecting the microbial inoculum, has small addition amount and hardly has influence on the quality of crude oil.

The invention has the advantages that:

the gasoline detergent disclosed by the invention is very environment-friendly and safe as a microbial preparation, does not harm the environment, is convenient to use, greatly reduces the quality of deposits in a modified gasoline combustor, consumes less oil, increases certain lubricating performance and has wide market potential.

Detailed Description

The following examples of the present invention are described in detail, and are only for the purpose of illustrating the present invention and are not to be construed as limiting the present invention.

Specific examples of the present invention are described below.

Example 1:

the preparation method of the gasoline biological detergent comprises the following steps:

(1) activated Rhodococcus erythropolis T7-3:

inoculating Rhodococcus erythropolis T7-3 strain in glycerol freezing tube, selecting 3 rings, inoculating into 80m L L B shake flask, shake culturing at 65 deg.C and 120rpm for 24h, and preparing into 10⁸CFU/ml bacterial liquid;

the L B culture medium formula is as follows:

according to the weight percentage, the peptone is 1 percent, the yeast powder is 0.5 percent, the NaCl is 1 percent, and the balance is purified water, and the pH value is 7.0;

(2) preparing a fermentation supernatant of marine salina bacteria L HOD-1:

culturing the frozen halophyte L HOD-1 in L B culture medium additionally containing 5 g/L of sucrose for 2 days, supplementing the sucrose every day during the culture, keeping the sucrose concentration of the culture medium at 4 g/L, centrifuging the culture at 5000r/min for 10min after the culture is finished, and collecting the supernatant;

(3) and (3) induction culture:

transferring the strain into an induction culture medium, inoculating 10 percent of the strain, and performing shaking culture for 24 hours;

the induction culture medium:

1% of liquid wax, 8% of fermentation supernatant of Haichilus halophila L HOD-1, 1.5% of yeast powder, 1% of sucrose, 0.5% of trehalose, 0.1% of sodium dihydrogen phosphate and 0.1% of potassium dihydrogen phosphate, wherein the pH value is 7.2;

(4) after induction culture, carrying out centrifugal treatment, and uniformly stirring to obtain thalli;

(5) preparing a gasoline biological detergent:

and (3) adding 1 time of the thalli obtained in the step (4) by weight into 1 time of the tween 80 by weight, 0.1 time of the fermentation supernatant of the marine salina L HOD-1 by weight, 5 times of oleic acid by weight, 1 time of trehalose by weight and 0.5 time of magnesium chloride by weight, and uniformly mixing to obtain thalli mixed oily liquid, namely the gasoline biological detergent.

The product can be used by shaking before use, and is optimally stored by refrigeration.

Example 2:

the preparation method of the gasoline biological detergent comprises the following steps:

(1) activated Rhodococcus erythropolis T7-3:

inoculating Rhodococcus erythropolis T7-3 strain in glycerol freezing tube, selecting 3 rings, inoculating into 80m L L B shake flask, shake culturing at 65 deg.C and 120rpm for 24 hr, and preparing into 10⁸CFU/ml bacterial liquid;

the L B culture medium formula is as follows:

according to the weight percentage, the peptone is 1 percent, the yeast powder is 0.5 percent, the NaCl is 1 percent, and the balance is purified water, and the pH value is 7.0;

(2) preparing a fermentation supernatant of marine salina bacteria L HOD-1:

culturing the frozen halophyte L HOD-1 in L B culture medium additionally containing 5 g/L of sucrose for 2 days, supplementing the sucrose every day during the culture, keeping the sucrose concentration of the culture medium at 5 g/L, centrifuging the culture at 5000r/min for 10min after the culture is finished, and collecting supernatant;

(3) and (3) induction culture:

transferring the strain into an induction culture medium, inoculating 10 percent of the strain, and performing shake culture for 36 hours;

the induction culture medium:

1% of liquid wax, 8% of fermentation supernatant of Haichilus halophila L HOD-1, 1.5% of yeast powder, 1% of sucrose, 0.5% of trehalose, 0.1% of sodium dihydrogen phosphate and 0.1% of potassium dihydrogen phosphate, wherein the pH value is 7.2;

(4) after induction culture, carrying out centrifugal treatment, and uniformly stirring to obtain thalli;

(5) preparing a gasoline biological detergent:

and (3) adding 1 time of the thalli obtained in the step (4) by weight into 1 time of the tween 80 by weight, 0.1 time of the fermentation supernatant of the marine salina L HOD-1 by weight, 5 times of oleic acid by weight, 1 time of trehalose by weight and 0.5 time of magnesium chloride by weight, and uniformly mixing to obtain thalli mixed oily liquid, namely the gasoline biological detergent.

The product can be used by shaking before use, and is optimally stored by refrigeration.

Example 3

Control 1:

(1) preparing a fermentation supernatant of marine salina bacteria L HOD-1:

culturing the frozen halophyte L HOD-1 in L B culture medium additionally containing 5 g/L of sucrose for 2 days, supplementing the sucrose every day during the culture, keeping the sucrose concentration of the culture medium at 5 g/L, centrifuging the culture at 5000r/min for 10min after the culture is finished, and collecting supernatant;

(2) preparing a gasoline biological detergent:

1 time of Tween 80, 0.1 time of fermentation supernatant of Haichilus halophila L HOD-1, 5 times of oleic acid, 1 time of trehalose and 0.5 time of magnesium chloride, and the components are uniformly mixed to obtain thallus mixed oily liquid, namely the gasoline biological detergent.

Control 2:

the method for preparing the gasoline biological detergent is the same as the method in example 1, only Rhodococcus erythropolis T7-3 strain is used as the only fermentation strain, the fermentation supernatant of the marine halophyte L HOD-1 is not prepared for induction culture, and the fermentation supernatant of the marine halophyte L HOD-1 is not added when the gasoline biological detergent is prepared.

Example 4

Mixing the gasoline biological detergent obtained in the examples 1-3 with 93# gasoline and a 1% sodium chloride solution according to the mass ratio of 1: 100: mixing at the ratio of 100, and stirring in a vortex at 50rpm for 36 hours; after the reaction is finished, standing for more than 2 hours for oil-water stratification, and then collecting an oil layer by using a separating funnel, wherein the oil product is the modified clean gasoline.

BYD 476ZQA gasoline engines were used to compare the amount of deposits and the fuel consumption rate in each group of combustion chambers.

The experimental conditions are as follows: each experimental group is 50 liters of prepared 93# gasoline, an engine is started under the conditions of room temperature of 20 ℃, air pressure of 101.7kPa and humidity of 60RH percent, a real-time measurement and control system is entered, the rotating speed of the engine is adjusted to the working condition of 2000r/min and load of 75 percent, the quality and the oil consumption rate of deposits in each group of combustion chambers are compared after the engine operates for 100 hours, each experiment is repeated for 3 times, and the specific test results are shown in table 1.

TABLE 1 Combustion Chamber deposits and specific Fuel consumption results for each group

As can be seen from Table 1, compared with the control group, the amount of the combustion chamber deposits added with the gasoline biological detergent of the example 1-2 is obviously less, the oil consumption rate is lower, the comparative example 2 group is slightly lower compared with the control group, and the comparative example 1 group is almost the same as the control group, which shows that only the Rhodococcus erythropolis T7-3 strain is used as the only fermentation strain, but the effect is not obvious, but the Rhodococcus erythropolis T7-3 is induced and cultured by adding the fermentation supernatant of the Halinosus L HOD-1, the crude oil can be modified, the amount of the deposits in the gasoline combustion chamber after modification is greatly reduced, and the oil consumption rate is lower.

Example 5

The gasoline bio-detergents obtained in examples 1 to 3 were used as an experimental group, and a four-ball test of a gasoline engine oil was directly conducted to compare the lubricating effect of the bio-detergents by using a Shell brand S L5W/30 as a comparative example.

The test data are shown in table 2:

TABLE 2 four-ball test scrub spot test

	Abrasion Spot diameter (mm)
		EXAMPLE 1 group	0.624
EXAMPLE 2 group	0.798
		Comparative example 1 group	1.837
Comparative example 2 group	1.715
		93# gasoline group	0.394

Although the effects of the example group and the comparative group are different from each other compared with the S L5W/30 lubricating oil, the example group is obviously better than the comparative group, and probably the Rhodococcus erythropolis T7-3 generates some anti-wear substances after the Rhodococcus erythropolis T7-3 is induced and cultured by adding the fermentation supernatant of the Halobacterium L HOD-1, so that the diameter of the abrasion is reduced.

In conclusion, the gasoline detergent obtained by adding the fermentation supernatant of the halophyte L HOD-1 to perform induction culture on the Rhodococcus erythropolis T7-3 can reduce the quality of deposits in a gasoline combustion chamber, reduce the oil consumption and increase certain lubricating performance.

It should be understood that the foregoing is only a preferred embodiment of the present invention.

Variations that do not depart from the gist of the invention are intended to be within the scope of the invention.

In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

While embodiments of the invention have been shown and described, it will be understood by those of ordinary skill in the art that: various changes, modifications, substitutions and alterations can be made to the embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the claims and their equivalents.

Claims (6)

1. A gasoline biological cleaning agent is characterized in that:

contains Rhodococcus erythropolis T7-3 and Haematococcus halophycus L HOD-1 fermentation supernatant.

2. The method for preparing the gasoline biological detergent as claimed in claim 1, which comprises the following steps:

(1) activated Rhodococcus erythropolis T7-3:

inoculating Rhodococcus erythropolis T7-3 strain in glycerol freezing tube, selecting 3 rings, inoculating into 80m L L B shake flask, shake culturing at 65 deg.C and 120rpm for 24h, and preparing into 10⁸CFU/ml bacterial liquid;

the L B culture medium formula is as follows:

according to the weight percentage, the peptone is 1 percent, the yeast powder is 0.5 percent, the NaCl is 1 percent, and the balance is purified water, and the pH value is 7.0;

(2) preparing a fermentation supernatant of marine salina bacteria L HOD-1:

culturing frozen halophyte L HOD-1 in L B culture medium additionally containing 5 g/L of sucrose for 2 days, supplementing sucrose every day during the culture, keeping the sucrose concentration of the culture medium at 4-5 g/L, centrifuging the culture at 5000r/min for 10min after the culture is finished, and collecting supernatant;

(3) and (3) induction culture:

transferring the strain into an induction culture medium, wherein the inoculation amount is 10%, and performing shaking culture for 24-36 h;

(4) after induction culture, carrying out centrifugal treatment, and uniformly stirring to obtain thalli;

(5) preparing the gasoline biological detergent.

3. The method of claim 2, wherein:

the induction medium of the step (3):

1% of liquid wax, 8% of fermentation supernatant of Haichi halophila L HOD-1, 1.5% of yeast powder, 1% of sucrose, 0.5% of trehalose, 0.1% of sodium dihydrogen phosphate, 0.1% of potassium dihydrogen phosphate, and the pH value of 7.2.

4. The method of claim 3, wherein the step (5) is:

and (3) adding 1 time of the thalli obtained in the step (4) by weight into 1 time of the tween 80 by weight, 0.1 time of the fermentation supernatant of the marine salina L HOD-1 by weight, 5 times of oleic acid by weight, 1 time of trehalose by weight and 0.5 time of magnesium chloride by weight, and uniformly mixing to obtain thalli mixed oily liquid, namely the gasoline biological detergent.

5. The gasoline biological detergent prepared by the preparation method of claims 2-4.

6. The use of the gasoline bio-detergent as set forth in claim 1 or 5, characterized in that the steps are as follows:

mixing the gasoline biological detergent with gasoline and a 1% sodium chloride solution according to the mass ratio of 1: 100: mixing at the ratio of 100, and stirring in a vortex at 50rpm for 36 hours; after the reaction is finished, standing for more than 2 hours for oil-water stratification, and then collecting an oil layer by using a separating funnel, wherein the oil product is the modified clean gasoline.