CN111495946B

CN111495946B - Restoration method for degrading resistance gene in polluted soil

Info

Publication number: CN111495946B
Application number: CN202010276925.4A
Authority: CN
Inventors: 吴丛杨慧; 孙加山; 朱开贞; 高连东; 张翔宇; 顾维; 李燕明
Original assignee: Suzhou Hongyu Environment Technology Co ltd
Current assignee: Suzhou Hongyu Environment Technology Co ltd
Priority date: 2020-04-10
Filing date: 2020-04-10
Publication date: 2021-02-19
Anticipated expiration: 2040-04-10
Also published as: CN111495946A

Abstract

The invention discloses a restoration method for degrading an resistance gene in polluted soil, which comprises the following steps: carrying out multistage crushing and screening on the contaminated soil to obtain soil particles with the particle size of less than 2 mm, and leaching the contaminated soil by using leacheate; separating mud and water from the slurry formed by mixing the soil particles smaller than 2 mm and the leacheate to obtain a mud cake and wastewater; loosening the mud cakes uniformly and removing water in the mud cakes to obtain uniform and dispersed fine soil particles; irradiating the fine soil particles by ultraviolet rays to degrade the resistance genes on the fine soil particles. The invention at least comprises the following advantages: and crushing, screening and leaching the contaminated soil to obtain fine-particle contaminated soil with the particle size of less than 2 mm and the rest soil repaired by the leacheate, adjusting the humidity of the fine-particle contaminated soil to a preset humidity range by using an electric heating mechanism, and adopting ultraviolet radiation to effectively degrade resistance genes on the fine-particle contaminated soil.

Description

Restoration method for degrading resistance gene in polluted soil

Technical Field

The invention relates to the technical field of polluted soil remediation, in particular to a remediation method for degrading an antibiotic gene in polluted soil.

Background

The statements in this section merely provide background information related to the present disclosure and may not constitute prior art.

Currently, antibiotics are abused seriously, which results in the increase of drug-resistant bacteria and the acceleration of the propagation speed, thereby causing the increase of the morbidity and the mortality of human infectious diseases. Therefore, Antibiotic Resistance Genes (ARGs) are considered as novel environmental pollutants. It has been shown that resistance genes are usually located on mobile genetic elements (mobilegenergicelemente) such as plasmids and transposons, and pathogenic bacteria can obtain ARGs from non-pathogenic environmental microorganisms and spread to indigenous environmental microorganisms by the action of gene level transfer (HGT). The environmental indigenous microorganisms present in the soil, surface water and groundwater thereby acquire the resistance genes and can multiply into a repository of resistance genes. Therefore, in recent years, attention is increasingly paid to pollution of ARGs in soil and antibiotic resistance brought by ARGs, and effective degradation of resistance genes in soil is one of the problems to be solved urgently in the environment.

The original means for research and control of the antagonistic genes mainly focuses on water bodies such as livestock and poultry manure, rivers, lakes, oceans and the like, and relatively few means for research and control of soil antibiotics are available. Research has shown that soil minerals have cross or combined action on the adsorption behavior of antibiotics, and organic matters in soil have uncertain relation of combination or rejection on antibiotics in different states. Research has been conducted on the effect of phytoremediation on the remediation of soil antibiotics, but phytoremediation or rhizosphere remediation has the problem of slow effectiveness, and the growth futures of plants, the concentration and the depth of polluted soil and the like all affect the phytoremediation effect.

Ultraviolet (UV) has wide application in the disinfection technology of water treatment, not only can inactivate the inactivation effect of antibiotic resistant bacteria, but also can effectively remove ARGs. But at present, the application of ultraviolet rays to the antibiotics in the polluted soil also begins to be developed. However, the ultraviolet wavelength effective for the degradation of the soil resistance gene belongs to short-wave sterilization ultraviolet and has weak penetrating power. And the problem of high humidity of the polluted soil after washing is solved, so that the adhesion phenomenon among particles is caused, and the subsequent ultraviolet degradation effect is reduced.

It should be noted that the above background description is only for the sake of clarity and complete description of the technical solutions of the present invention and for the understanding of those skilled in the art. Such solutions are not considered to be known to the person skilled in the art merely because they have been set forth in the background section of the invention.

Disclosure of Invention

In order to overcome the defects in the prior art, the embodiment of the invention provides a repair method for degrading resistance genes in contaminated soil, which comprises the steps of crushing, screening and leaching the contaminated soil to obtain fine-particle contaminated soil with the particle size of less than 2 mm and other soil repaired by tap water, adjusting the humidity of the fine-particle contaminated soil to be within a preset humidity range through an electric heating mechanism, and effectively degrading the resistance genes in the fine-particle contaminated soil by adopting the action of ultraviolet radiation.

The embodiment of the application discloses: a restoration method for degrading an resistance gene in polluted soil comprises the following steps:

carrying out multistage crushing and screening on the contaminated soil to obtain soil granules with the grain size of less than 2 mm, and simultaneously leaching the contaminated soil subjected to crushing and screening at each stage by using tap water, wherein the tap water is used for eluting resistance genes on the soil granules in the multistage crushing and screening process, so that the resistance genes on the soil granules with the grain size of more than or equal to 2 mm are dissolved in the tap water;

wherein, in the step of carrying out multi-stage crushing and screening on the polluted soil, the method comprises a first-stage crushing and screening process and a second-stage crushing and screening process;

the first stage crushing and screening process comprises the following steps: respectively conveying the 18 mm soil granules and tap water into a soil scrubbing machine, fully and uniformly mixing the 18 mm soil granules and the tap water through the soil scrubbing machine, and eluting the clay granules and the resistance genes adhered to the 18 mm soil granules;

the second stage crushing and screening process comprises the following steps: conveying the contaminated soil treated in the first-stage crushing and screening process to a soil hydraulic vibrating screen, and screening the contaminated soil into three grades with the grain diameters of more than 10 mm, between 2 and 10 mm and less than 2 mm through the soil hydraulic vibrating screen, wherein the contaminated soil with the grain diameter of more than 10 mm is conveyed to a gravel pile, the contaminated soil with the grain diameter of between 2 and 10 mm is conveyed to a spiral sand washer, and the contaminated soil with the grain diameter of less than 2 mm and the washed aqueous solution are conveyed to a filter press;

the mud and water separation is realized by the mud prepared by mixing the soil particles smaller than 2 mm and tap water, and a mud cake and wastewater are obtained;

loosening the mud cakes uniformly and removing water in the mud cakes to obtain uniform and dispersed fine soil particles, wherein the method comprises the following steps: the humidity of the fine particle polluted soil can be adjusted to be within a preset humidity range through the electric heating mechanism;

the electric heating mechanism comprises a cylindrical shell, a plurality of rotary scrapers arranged in the shell and a heating coil arranged in the shell, and particularly, the shell is provided with a sealed cavity, the top of the shell is provided with a feed inlet communicated with the sealed cavity, and the bottom of the shell is provided with a discharge outlet communicated with the sealed cavity;

irradiating the fine soil particles by ultraviolet rays to degrade the resistance genes on the fine soil particles.

Further, in the step of "irradiating the fine soil particles with the ultraviolet lamp", there is further included applying the fine soil particles with a high frequency vibrator so that the fine soil particles can roll with respect to the ultraviolet lamp.

Further, before the step of performing multistage crushing and screening on the contaminated soil, the method comprises the steps of primarily screening the contaminated soil to remove impurities with large particle size, and crushing the primarily screened contaminated soil to soil particles of 18 mm through an ALLU hopper.

Further, in the step "the second crushing and screening step", tap water is added to the screw sand washer, the resistance genes on the contaminated soil having a particle size of 2 to 10 mm are eluted by the screw sand washer, and the aqueous solution after the washing is transferred to a filter press.

Further, in the step of obtaining the mud cake and the waste water, the following procedures are included:

introducing the wastewater into a coagulating sedimentation tank, and adding a coagulant into the wastewater according to the mass ratio of 1:1000, so that the turbidity of the wastewater after coagulating sedimentation is less than 20 NTU;

removing suspended solids in the wastewater after coagulating sedimentation through a grid, introducing the suspended solids into an ultraviolet water treatment device, and radiating the wastewater through ultraviolet rays with the wavelength of 250-260nm generated by the ultraviolet lamp water treatment device, wherein the radiation time is between 50 and 70S;

and (3) carrying out plate-and-frame filter pressing on the coagulating sedimentation in the coagulating sedimentation tank to realize mud-water separation to obtain mud cakes and wastewater, and collecting and treating the wastewater.

By means of the technical scheme, the invention has the following beneficial effects: firstly, crushing, screening and leaching the contaminated soil to obtain fine-particle contaminated soil with the particle size of less than 2 mm and the rest soil repaired by tap water, wherein the humidity of the fine-particle contaminated soil can be adjusted to be within a preset humidity range through an electric heating mechanism, and resistance genes on the fine-particle contaminated soil can be effectively degraded by adopting the action of ultraviolet radiation.

In order to make the aforementioned and other objects, features and advantages of the invention comprehensible, preferred embodiments accompanied with figures are described in detail below.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.

FIG. 1 is a flow chart of a method in an embodiment of the invention;

FIG. 2 is an abundance table of contaminated soil in example 1 of the present invention;

FIG. 3 is an abundance table of contaminated soil in example 2 of the present invention;

fig. 4 is a schematic view of the overall structure of an electric heating mechanism in the embodiment of the invention;

FIG. 5 is a partial schematic view of an electrical heating mechanism in an embodiment of the present invention;

FIG. 6 is a partial schematic view of an electric heating mechanism in an embodiment of the present invention;

FIG. 7 is a schematic view of the overall structure of a UV lamp degradation mechanism in an embodiment of the invention;

FIG. 8 is a schematic view of a partial structure of a UV lamp degradation mechanism in an embodiment of the invention.

Reference numerals of the above figures: 1. a housing; 2. rotating the scraper plate; 3. a heating coil; 4. a feed inlet; 5. a discharge port; 6. a stirring area; 7. a conveying member; 8. a vibrating member; 9. a seal member; 10. an ultraviolet lamp; 11. an operation panel. .

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

It should be noted that, in the description of the present invention, the terms "first", "second", and the like are used for descriptive purposes only and for distinguishing similar objects, and no precedence between the two is considered as indicating or implying relative importance. In addition, in the description of the present invention, "a plurality" means two or more unless otherwise specified.

Referring to fig. 1 to 3, this embodiment discloses a remediation method for degrading an antibiotic in contaminated soil, which includes a multi-stage crushing and screening mechanism, a mud-water separating mechanism, an electric heating mechanism, and an ultraviolet lamp degradation mechanism. The above mechanism can degrade the resistance gene in the contaminated soil, wherein the degradation step comprises:

carrying out multistage crushing and screening on the contaminated soil through a multistage crushing and screening mechanism to obtain soil granules with the grain diameter of less than 2 mm, and simultaneously leaching the contaminated soil subjected to each stage of crushing and screening through tap water, wherein the tap water is used for eluting resistance genes on the soil granules in the multistage crushing and screening process, so that the resistance genes on the soil granules with the grain diameter of more than or equal to 2 mm are dissolved in the tap water

Mud-water separation is realized through the mud-water separation mechanism for the mud formed by mixing the soil particles smaller than 2 mm and tap water, so that mud cakes and waste water are obtained;

loosening the mud cakes uniformly through an electric heating mechanism and removing water in the mud cakes to obtain uniform and dispersed fine soil particles;

and irradiating the fine soil particles by ultraviolet rays generated by the ultraviolet lamp mechanism through the ultraviolet lamp degradation mechanism so as to degrade the resistance genes on the fine soil particles.

From the physical and chemical properties of soil, the soil is composed of particles with different particle sizes, the smaller the particle size, the larger the specific surface area of the particles, most pollutants in general soil are gathered and enriched in fine particles, and the more resistance genes are gathered and enriched in the soil along with the reduction of the particle size. In order to solve the above problem, in the method, the multi-level crushing and screening mechanism can divide the non-yielding soil into three levels of grain sizes of more than 10 mm, 2-10 mm and less than 2 mm.

In the method, tap water in the multi-stage screening and crushing mechanism adopts a physical leaching mode, so that components such as gravels, sandy soil, clay and the like in pollution can be separated, and resistance genes loosely attached to the surfaces of large-particle and medium-particle soil can be eluted to be dissolved in the tap water. The used tap water can be recycled or discharged after reaching the standard after being treated by the water tight treatment mechanism.

Example 1:

the contaminated soil to be tested was from a site where certain medical waste was stockpiled in Suzhou.

The resistance genes contained in the polluted soil mainly comprise aminoglycosides, chloramphenicol, sulfonamides and tetracyclines. Wherein the abundance of aminoglycosides in the contaminated soil before degradation is 0.0266, the abundance of chloramphenicols is 0.0153, the abundance of sulfonamides is 0.0397, and the abundance of tetracyclines is 0.00508.

Firstly, preprocessing the polluted soil, including primarily screening to remove impurities with large grain size, and crushing the primarily screened polluted soil to soil grains with grain size of about 18 mm through an ALLU hopper.

And conveying the pretreated soil granules with the grain size of about 18 mm into a soil scrubbing machine, and performing a first-stage crushing and screening process, wherein in the working process of the soil scrubbing machine, tap water (tap water) is applied to the soil granules with the grain size of about 18 mm, so that the soil granules can be fully and uniformly mixed with the tap water, and then, the clay granules and the resistance genes attached to the surfaces of the soil granules are preliminarily eluted, and the eluted resistance genes are dissolved in the tap water.

And conveying the soil grains subjected to the first-stage crushing and screening process to a soil hydraulic vibrating screen, and further performing the second-stage crushing and screening process, wherein the soil hydraulic vibrating screen is used for crushing the soil grains in the working process, screening the soil grains with different grain diameters to obtain three grades with the grain diameters of more than 10 mm, 2-10 mm and less than 2 mm, conveying the contaminated soil with the grain diameter of more than 10 mm to a gravel pile, conveying the contaminated soil with the grain diameter of 2-10 mm to a spiral sand washer, and conveying the contaminated soil with the grain diameter of less than 2 mm and the washed aqueous solution to a filter press.

In the above process, preferably, tap water is added to the screw sand washer, the resistance genes on the contaminated soil having a particle size of 2 to 10 mm are eluted by the screw sand washer, and the washed aqueous solution is transferred to the filter press. By adopting the mode, the resistance genes attached to the surfaces of the soil grains with different grain diameters can be well eluted.

Through the treatment steps, the values of aminoglycosides, chloramphenicol, sulfonamides and tetracyclines attached to the soil particles with the particle size of more than 10 mm are all 0; the number of aminoglycosides attached to the granules of clay having a particle size of 2-10 mm was 0.00166, chloramphenicol was 0, sulfonamides was 0.002106, and tetracyclines was 0.001555.

And conveying the slurry formed by the processed soil particles with the particle size of less than 2 mm and tap water to a sludge-water separation mechanism, wherein the sludge-water separation mechanism is used for performing sludge-water separation on the slurry in a plate type pressure filtration manner to obtain a sludge cake and wastewater, and collecting and processing the wastewater.

The mud cake has the defect of high humidity, and if an ultraviolet lamp degradation mechanism is directly adopted for treatment, the defect of low efficiency of removing the resistance genes attached to the soil particles is caused due to the defect of weak penetrating capability of the ultraviolet lamp. And because of the problem of high humidity, the action of flattening the soil particles is difficult to be finished.

Aiming at the technical problems, the mud cakes are conveyed to the electric heating mechanism, and the electric heating mechanism can loosen the mud cakes uniformly and remove water in the mud cakes to obtain uniform and dispersed fine soil particles.

Conveying the fine soil particles to an ultraviolet lamp degradation mechanism, wherein the fine soil particles are spread on a conveyor, the thickness of the fine soil particles is controlled to be 5 mm, the fine soil particles are conveyed at a conveying speed of 20m/s, and the fine soil particles are irradiated by ultraviolet rays to degrade resistance genes on the fine soil particles; the wavelength of the selected ultraviolet is 254nm, and the radiation intensity of the ultraviolet is controlled to be more than 1000uw/cm²

Through the degradation process, the aminoglycoside value, the chloramphenicol value, the sulfonamide value and the tetracycline value attached to the soil particles with the particle size of less than 2 mm were 0.004592, 0.0008, 0.000981 and 0.001529, respectively.

Example 2:

the contaminated soil to be tested was from a medical waste disposal site in Kunshan.

The resistance genes contained in the polluted soil mainly comprise aminoglycosides, chloramphenicol, sulfonamides and tetracyclines. Wherein the abundance of aminoglycosides in the contaminated soil before degradation is 0.0276, the abundance of chloramphenicols is 0.0183, the abundance of sulfonamides is 0.0367, and the abundance of tetracyclines is 0.00538.

Through the treatment steps, the values of aminoglycosides, chloramphenicol, sulfonamides and tetracyclines attached to the soil particles with the particle size of more than 10 mm are all 0; the aminoglycoside value adhered to the granules having a particle size of 2-10 mm was 0.00176, the chloramphenicol value was 0, the sulfonamide value was 0.002156, and the tetracycline value was 0.001573.

Conveying the fine soil particles to an ultraviolet lamp degradation mechanism, wherein the fine soil particles are spread on a conveyor, the thickness of the fine soil particles is controlled to be 2 mm, the fine soil particles are conveyed at a conveying speed of 6m/s, and the fine soil particles are irradiated by ultraviolet rays to degrade resistance genes on the fine soil particles; the wavelength of the selected ultraviolet is 254nm, and the radiation intensity of the ultraviolet is controlled to be more than 1000uw/cm²

Through the degradation process, the aminoglycoside value, the chloramphenicol value, the sulfonamide value and the tetracycline value attached to the soil particles with the particle size of less than 2 mm are 0.004873, 0.0000, 0.000783 and 0.001029, respectively.

In conclusion, different conveying speeds and spreading thicknesses of soil particles can be selected according to different resistance genes so as to achieve the optimal degradation effect.

In the two embodiments, the treatment of the obtained wastewater specifically includes:

removing suspended solids in the wastewater after coagulating sedimentation through a grid, introducing the suspended solids into an ultraviolet water treatment device, and radiating the wastewater through ultraviolet rays with the wavelength of 250-260nm generated by the ultraviolet lamp water treatment device, wherein the radiation time is between 50 and 70S.

After the treatment, the values of aminoglycosides, chloramphenicol, sulfonamides and tetracyclines in the wastewater are all 0.

As shown in fig. 4 to 6, the electric heating mechanism includes a cylindrical housing 1, a plurality of rotary blades 2 provided in the housing 1, and a heating coil 3 provided in the housing 1. Specifically, the casing 1 has a sealed cavity, a feed inlet 4 communicating with the sealed cavity is arranged on the top of the casing 1, and a discharge outlet 5 communicating with the sealed cavity is arranged on the bottom of the casing 1. The mud cake can be conveyed into the sealed cavity of the shell 1 through the feed inlet 4.

The casing 1 is internally provided with a rotating shaft extending along the axis of the rotating shaft, the fixed ends of the rotating scrapers 2 are respectively and fixedly arranged on the rotating shaft, and the rotating scrapers 2 are uniformly distributed around the rotating shaft in the circumferential direction. The free end of each rotary scraper 2 can be abutted against the inner side wall of the shell 1, the lower end face of each rotary scraper 2 is abutted against the bottom of the shell 1, and through the structural arrangement, an independent stirring area 6 for containing mud cakes can be formed between every two adjacent rotary scrapers 2. Preferably, each rotating scraper 2 is driven by the rotating shaft to rotate continuously, so that the stirring area 6 can be communicated with the feeding hole 4 and the discharging hole 5 in sequence during rotation.

Foretell heating coil 3 sets up in the top position department of casing 1, in order to be in mud cake in the sealed cavity lasts the heating, the cooperation the rotation that rotatory scraper blade 2 lasts realizes the stirring, and then can effectively get rid of unnecessary moisture in the mud cake for pollute soil has the mud cake state to convert the loose form, thereby can realize the tiling action to polluting soil conveniently.

Referring to fig. 7 and 8, in this embodiment, the ultraviolet lamp degradation mechanism is used for abutting against the discharge port 5, and includes a conveying member 7 for bearing contaminated soil formed by loosening mud cakes, a vibrating member 8 connected to the conveying member 7, a sealing member 9 disposed on the conveying member 7 along the conveying direction of the contaminated soil, and a plurality of ultraviolet lamps 10 disposed in the sealing member 9.

Specifically, the conveying member 7 includes a conveyor belt made of a PVC material, and since the conveyor belt is used for conveying contaminated soil containing resistance genes, the PVC material has a characteristic of being not decomposed by bacteria and fungi, so that the contaminated soil can be effectively conveyed for a long time without causing a reverse pollution to the contaminated soil.

Preferably, the conveying speed of the conveying belt is set to be 6-20m/s, the width of the conveying belt is set to be about 800mm, and the structure is set to ensure that the whole structure of the conveying piece 7 is small and small, and meanwhile, the contaminated soil on the conveying piece 7 can be efficiently repaired by controlling the conveying speed and matching with a repairing mechanism.

The vibrating member 8 is preferably a high-frequency vibrator, which can act on the entire conveyor belt or only on the position of the conveyor belt corresponding to the radiation mechanism. The high-frequency vibrator has the characteristics of high frequency and small amplitude, so that the polluted soil at the action position of the high-frequency vibrator has the motion states of vibration and rolling, all surfaces of the polluted soil can be effectively radiated by the repairing mechanism, and resistance genes on the polluted soil are effectively removed.

In another possible embodiment, in order to avoid the above-mentioned phenomenon that the contaminated soil falls off from the conveyor belt during vibration, baffle members are provided at both sides of the conveyor belt in the soil conveying direction to block the vibrating soil, so that the contaminated soil is always on the conveyor belt during conveyance.

The sealing element 9 includes a housing 1 having a sealed cavity, and the housing 1 has an input port and an output port through which the conveyor 7 passes, so that the conveyor 7 can convey the contaminated soil into the sealed cavity to cooperate with the ultraviolet lamp 10 to radiate the contaminated soil.

The ultraviolet lamps 10 are all arranged at the top of the shell 1, preferably, the ultraviolet lamps 10 are 25 in number and distributed in a rectangular shape, so that the polluted soil can be uniformly radiated. Wherein, the radiation intensity of the ultraviolet lamp 10 is more than 1000uw/cm2, and the wavelength is 254nm preferably. Here, since the ultraviolet lamp 10 of the above specification is weak in the ability to penetrate the soil, the thickness of the soil laid on the conveyor belt is controlled to be 2 to 6 mm in order to more efficiently remove the resistance genes in the contaminated soil.

In the above arrangement, each of the ultraviolet lamps 10 has a lighting power supply, wherein the lighting power supply includes a ballast of 30W. Preferably, each of the ultraviolet lamps 10 is independently operable, thereby enabling the ultraviolet lamps 10 to generate different radiation intensities to accommodate different radiation requirements of the contaminated soil.

In the present embodiment, the sealing member 9 is provided with a cooling fan that can communicate with the outside to cool the inside of the sealing member 9. Specifically, an exhaust port matched with the cooling fan is arranged in the middle of the upper portion of the housing 1, and the cooling fan can convey hot air flow in the sealed cavity of the housing 1 to the outside from the exhaust port, so that the temperature in the sealed cavity of the housing 1 is ensured to be within a temperature range required by normal irradiation.

In the present embodiment, the sealing member 9 is further provided with a dust removing member capable of communicating with the outside to bring the dust inside the sealing member 9 to the outside. Specifically, this dust removal spare is including setting up the cover that induced drafts of feed inlet 4 department of casing 1 discharges impurity such as the dust that produces in polluting soil and the external world in the vibration extremely sealed cavity's outside, and then guarantee the inside cleanliness factor of sealed cavity.

In this embodiment the device comprises a controller and an operating panel 11 for access control of the device. The controller is respectively in signal connection with the parts such as the conveying mechanism, the repairing mechanism, the cooling fan and the dust removing part which realize the functions, so that an operator can respectively control the actions of the parts through the operation panel 11, and the intelligence of the whole equipment is improved.

The whole integration degree of the equipment is high due to the structure arrangement, and the ordered completion of actions can be guaranteed only by supplying power to the equipment in the whole structure operation. In conclusion, the equipment formed by the mechanism can be conveniently moved to a required position as mobile equipment.

The principle and the implementation mode of the invention are explained by applying specific embodiments in the invention, and the description of the embodiments is only used for helping to understand the method and the core idea of the invention; meanwhile, for a person skilled in the art, according to the idea of the present invention, there may be variations in the specific embodiments and the application scope, and in summary, the content of the present specification should not be construed as a limitation to the present invention.

Claims

1. A restoration method for degrading an resistance gene in polluted soil is characterized by comprising the following steps:

the electric heating mechanism comprises a cylindrical shell, a plurality of rotary scrapers arranged in the shell and a heating coil arranged in the shell, wherein the shell is provided with a sealed cavity, the top of the shell is provided with a feed inlet communicated with the sealed cavity, and the bottom of the shell is provided with a discharge outlet communicated with the sealed cavity;

2. The method for restoring a resistance gene in a degraded and contaminated soil according to claim 1, wherein in the step of irradiating said fine soil particles with an ultraviolet lamp, further comprising applying said fine soil particles with a high-frequency vibrator so that said fine soil particles can roll against the ultraviolet lamp.

3. The method for restoring resistance genes in degraded contaminated soil according to claim 1, wherein before the step of "subjecting the contaminated soil to multistage crushing and screening", the method comprises preliminarily screening the contaminated soil to remove impurities with large particle size, and crushing the preliminarily screened contaminated soil into soil particles of 18 mm by using an ALLU bucket.

4. The method for repairing a resistance gene in degraded and contaminated soil according to claim 1, wherein in the "second-stage crushing and screening step", tap water is added to the screw sand washer, the resistance gene on the contaminated soil having a particle size of 2 to 10 mm is eluted by the screw sand washer, and the aqueous solution after the elution is sent to a filter press.

5. The method for restoring resistance genes in degraded and polluted soil according to claim 1, wherein the step of obtaining mud cake and wastewater comprises the following steps: