CN111494442A - Method for improving extraction yield of total saponins in akebia trifoliata peels - Google Patents
Method for improving extraction yield of total saponins in akebia trifoliata peels Download PDFInfo
- Publication number
- CN111494442A CN111494442A CN202010487718.3A CN202010487718A CN111494442A CN 111494442 A CN111494442 A CN 111494442A CN 202010487718 A CN202010487718 A CN 202010487718A CN 111494442 A CN111494442 A CN 111494442A
- Authority
- CN
- China
- Prior art keywords
- akebia trifoliata
- peels
- akebia
- enzymolysis
- total saponins
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Public Health (AREA)
- Pharmacology & Pharmacy (AREA)
- Veterinary Medicine (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Animal Behavior & Ethology (AREA)
- Organic Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Alternative & Traditional Medicine (AREA)
- Gastroenterology & Hepatology (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Epidemiology (AREA)
- Communicable Diseases (AREA)
- Oncology (AREA)
- Steroid Compounds (AREA)
Abstract
A method for improving the extraction of total saponins in akebia trifoliata peels comprises the following steps: mixing the akebia trifoliata peel powder with pectinase, cellulase, amylase and polyether serving as an enzyme activator, and placing the mixture into a reaction tank for enzymolysis to obtain akebia trifoliata peel powder enzymatic hydrolysate; adding 70% methanol into the akebia trifoliata peel powder enzymolysis liquid, and performing ultrasonic extraction to obtain an extracting solution and filter residues; adsorbing the extract by macroporous resin, washing, eluting, and collecting Akebia trifoliata peel powder eluate; adjusting the pH value of the eluent, and concentrating and recycling the eluent; freeze drying to obtain the total saponin extract in the pericarp of akebia trifoliata. Compared with the traditional method for extracting the akebia trifoliata peels by alcohol, the akebia trifoliata peels are prepared into akebia trifoliata peel powder, and are subjected to enzymolysis by pectinase, cellulase, amylase and polyether serving as an enzyme activator to obtain akebia trifoliata enzymatic hydrolysate for extraction, so that the extraction yield of the total saponins as the active ingredients in the akebia trifoliata peels is effectively improved.
Description
Technical Field
The invention belongs to the technical field of traditional Chinese medicine extraction, and particularly relates to a method for improving the extraction yield of total saponins in akebia trifoliata peels.
Background
In recent years, the production and extraction methods of Chinese herbal medicines are developed and mechanized to a certain extent, and research papers on the extraction methods of Chinese herbal medicines are also provided, however, the traditional Chinese herbal medicines are considered to have more impurities and have unstable and lower active ingredient content, and can not be in contact with modern medicine, thereby restricting the development of modern Chinese medicines.
The akebia trifoliata peels have complex chemical components and various structures, mainly comprise triterpenes and glycosides thereof, and triterpenoid saponin compounds are separated from akebia trifoliata peels at present, wherein the aglycone types comprise hederagenin, norhederagenin, oleanane sapogenin, noroleanane sapogenin, argatropurpurin, ursolic sapogenin and the like. Has antibacterial, antiinflammatory, tumor growth inhibiting, hepatoprotective, enzyme reducing, and immunity improving effects. The traditional method for extracting the effective components of the akebia trifoliata peels is an alcohol extraction method, and the extraction rate of the total saponins in the akebia trifoliata peels is low. The extraction of effective substances from traditional plants is an important unit operation and is the starting point in the research of most traditional Chinese herbal medicines. The quality of the extraction method and the achievement is directly related to the difficulty of the subsequent experiment progress. The extraction method can be regarded as an important link of modern Chinese herbal medicine experiments. Therefore, it is necessary to research and optimize the extraction method of the effective substances of the traditional Chinese herbal medicines.
Disclosure of Invention
The invention aims to provide a method capable of effectively extracting the total saponin extraction yield from akebia trifoliata peels.
The invention is realized by the following technical scheme:
q1: cleaning caulis Akebiae pericarp, sun drying, slicing, pulverizing, and sieving with 40 mesh sieve to obtain refined caulis Akebiae pericarp powder;
q2: adding 0.02-0.05 g of pectinase, 0.02-0.05 g of cellulase and 0.02-0.05 g of amylase into each kilogram of akebia trifoliata peel powder respectively, adding an enzyme activator polyether into the mixture according to the mass ratio of 0.3-0.5% of pectinase, mixed enzyme of cellulase and amylase and the enzyme activator polyether, uniformly mixing the materials, and carrying out enzymolysis in a reaction tank to obtain akebia trifoliata peel enzymatic hydrolysate;
q3: adding 70% methanol into the akebia trifoliata peel enzymatic hydrolysate according to the volume ratio of 1: 6-10, fully stirring and extracting, separating an extracting solution and filter residues, and repeating for 2-3 times;
q4: sequentially adsorbing, washing and eluting the extracting solution, and collecting akebia trifoliata peel powder eluent; concentrating the eluate, and freeze drying to obtain total saponin extract.
And the slicing and crushing in the step Q1 is to cut the akebia trifoliata peels into 3-5 mm slices and then put the akebia trifoliata peels into a traditional Chinese medicine crusher for crushing.
And the enzymolysis temperature of the step Q2 is 35-55 ℃, enzymolysis is carried out in the reaction tank for 2-3 h, then the pressure in the reaction tank is restored to be in a normal pressure state, and the akebia trifoliata peel enzymolysis liquid is obtained.
The enzymolysis in the steps is carried out for 0.5h at 35 ℃, 1h at 45 ℃ and 1h at 55 ℃ in sequence.
The pressure in the reaction tank in the enzymolysis process is 0.4-0.6 Mpa, and the pH value is 4-5.
The temperature of the methanol added in the step Q3 is 70-90 ℃.
Adsorbing and enriching by using a macroporous resin adsorption method in the step Q4, washing by using 40% ethanol and purified water, and eluting by using 95% ethanol solution to realize concentration to obtain an eluent; adjusting the pH value of the eluent to 2-4, and concentrating and recovering the eluent at 40-60 ℃ for 60-90 min to obtain a concentrated solution.
Compared with the prior art, the invention has the following beneficial technical effects:
compared with the traditional method for extracting the total saponins of the active ingredients in the akebia trifoliata peel powder, the method provided by the invention has the advantages that the akebia trifoliata peel is prepared into the akebia trifoliata peel powder, and the akebia trifoliata peel enzymatic hydrolysate is obtained through the enzymolysis of pectinase, cellulase, amylase and an enzyme activator polyether and then extracted, so that the extraction yield of the total saponins of the active ingredients in the akebia trifoliata peel of the traditional Chinese medicine is effectively improved.
Detailed Description
Specific examples are given below.
Example 1:
q1: cleaning caulis Akebiae pericarp, sun drying, cutting into 3mm pieces, pulverizing in a traditional Chinese medicine pulverizer, sieving with 40 mesh micro powder vibration sieve to obtain refined caulis Akebiae pericarp powder;
q2: carrying out enzymolysis reaction on akebia trifoliata peel powder; the method specifically comprises the following steps:
q21: adding 0.05g of pectinase, 0.05g of cellulase and 0.05g of amylase into each kilogram of akebia trifoliata peel powder respectively, and then adding an enzyme activator polyether into the mixture according to the mass ratio of 0.5 percent of pectinase, mixed enzyme of cellulase and amylase and the enzyme activator polyether to uniformly mix to obtain an enzyme mixture;
q22: uniformly paving the enzyme mixture in a reaction tank, and closing the reaction tank;
q23: adjusting the temperature in the reaction tank to 35 ℃, the pressure to 0.5MPa, the pH value to 4.5, and carrying out enzymolysis for 0.5 h; adjusting the temperature in the reaction tank to 45 ℃, the pressure to 0.5MPa, the pH value to 4.5, and carrying out enzymolysis for 1 h; adjusting the temperature in the reaction tank to 55 ℃, the pressure to 0.5MPa, the pH value to 4.5, and carrying out enzymolysis for 1 h; then recovering the pressure in the reaction tank to normal pressure to obtain akebia trifoliata peel enzymatic hydrolysate;
q3: adding 70% methanol at 70 deg.C into caulis Akebiae hydrolysate at a volume ratio of 1:8, stirring and extracting, separating extractive solution and residue, and repeating for 3 times;
q4: adsorbing and enriching the eluent by using a macroporous resin adsorption method, washing the eluent by using ethanol with the volume concentration of 40% and purified water, and eluting the eluent by using ethanol solution with the volume concentration of 95% to realize concentration to obtain eluent; adjusting pH of the eluate to 3, concentrating at 50 deg.C for 75min, recovering, and freeze drying to obtain total saponin extract.
The extraction rate of the total saponins in the pericarp of akebia trifoliata is calculated by the following formula:
extraction yield/% -% m1/m0×100
In the formula: m is1The mass/g of the akebia trifoliata peel total saponins is as follows: m is0Is the mass/g of the raw material.
The total saponin content was measured with HP L C, and the results showed that the total saponin extraction rate was 0.64%.
Example 2:
q1: cleaning caulis Akebiae pericarp, sun drying, cutting into 4mm pieces, pulverizing in a traditional Chinese medicine pulverizer, sieving with 40 mesh micro powder vibration sieve to obtain refined caulis Akebiae pericarp powder;
q2: carrying out enzymolysis reaction on akebia trifoliata peel powder; the method specifically comprises the following steps:
q21: adding 0.02g of pectinase, 0.02g of cellulase and 0.02g of amylase into each kilogram of akebia trifoliata peel powder respectively, and then adding an enzyme activator polyether into the mixture according to the mass ratio of 0.4 percent of pectinase, mixed enzyme of cellulase and amylase and the enzyme activator polyether to uniformly mix to obtain an enzyme mixture;
q22: uniformly paving the enzyme mixture in a reaction tank, and closing the reaction tank;
q23: adjusting the temperature in the reaction tank to 35 ℃, the pressure to 0.4MPa, the pH value to 4, and carrying out enzymolysis for 0.5 h; adjusting the temperature in the reaction tank to 45 ℃, the pressure to 0.4MPa, the pH value to 4, and carrying out enzymolysis for 1 h; adjusting the temperature in the reaction tank to 55 ℃, the pressure to 0.4MPa, the pH value to 4, and carrying out enzymolysis for 1 h; then recovering the pressure in the reaction tank to normal pressure to obtain akebia trifoliata peel enzymatic hydrolysate;
q3: adding 70% methanol with the concentration of 80 ℃ into the akebia trifoliata peel enzymolysis liquid according to the volume ratio of 1:6, fully stirring and extracting, separating the extracting solution and filter residue, and repeating for 2 times;
q4: adsorbing and enriching the eluent by using a macroporous resin adsorption method, washing the eluent by using ethanol with the volume concentration of 40% and purified water, and eluting the eluent by using ethanol solution with the volume concentration of 95% to realize concentration to obtain eluent; adjusting pH of the eluate to 2, concentrating at 60 deg.C, recovering for 60min to obtain concentrated solution, and freeze drying to obtain total saponin extract.
The extraction rate of the total saponins in the pericarp of akebia trifoliata is calculated by the following formula:
extraction yield/% -% m1/m0×100
In the formula: m is1The mass/g of the akebia trifoliata peel total saponins is as follows: m is0Is the mass/g of the raw material.
The total saponin content was analyzed by HP L C, and the results showed that the total saponin extraction rate was 0.57%.
Example 3:
q1: cleaning caulis Akebiae pericarp, sun drying, cutting into 5mm pieces, pulverizing in a traditional Chinese medicine pulverizer, sieving with 40 mesh micro powder vibration sieve to obtain refined caulis Akebiae pericarp powder;
q2: carrying out enzymolysis reaction on akebia trifoliata peel powder; the method specifically comprises the following steps:
q21: adding 0.03g of pectinase, 0.03g of cellulase and 0.03g of amylase into each kilogram of akebia trifoliata peel powder respectively, and then adding an enzyme activator polyether into the mixture according to the mass ratio of 0.3 percent of pectinase, mixed enzyme of cellulase and amylase and the enzyme activator polyether to uniformly mix to obtain an enzyme mixture;
q22: uniformly paving the enzyme mixture in a reaction tank, and closing the reaction tank;
q23: adjusting the temperature in the reaction tank to 35 ℃, the pressure to 0.6MPa, the pH value to 5, and carrying out enzymolysis for 0.5 h; adjusting the temperature in the reaction tank to 45 ℃, the pressure to 0.6MPa, the pH value to 5, and carrying out enzymolysis for 1 h; adjusting the temperature in the reaction tank to 55 ℃, the pressure to 0.6MPa, the pH value to 5, and carrying out enzymolysis for 1 h; then recovering the pressure in the reaction tank to normal pressure to obtain akebia trifoliata peel enzymatic hydrolysate;
q3: adding 70% methanol at 90 deg.C into caulis Akebiae peel enzymolysis solution at volume ratio of 1:10, stirring and extracting, separating extractive solution and residue, and repeating for 3 times;
q4: adsorbing and enriching the eluent by using a macroporous resin adsorption method, washing the eluent by using ethanol with the volume concentration of 40% and purified water, and eluting the eluent by using ethanol solution with the volume concentration of 95% to realize concentration to obtain eluent; adjusting pH of the eluate to 4, concentrating at 40 deg.C, recovering for 90min to obtain concentrated solution, and freeze drying to obtain total saponin extract.
The extraction rate of the total saponins in the pericarp of akebia trifoliata is calculated by the following formula:
extraction yield/% -% m1/m0×100
In the formula: m is1The mass/g of the akebia trifoliata peel total saponins is as follows: m is0Is the mass/g of the raw material.
The total saponin content was measured with HP L C, and the results showed that the total saponin extraction yield was 0.53%.
Claims (7)
1. A method for improving the extraction yield of total saponins in akebia trifoliata peels is characterized by comprising the following steps:
q1: cleaning caulis Akebiae pericarp, sun drying, slicing, pulverizing, and sieving with 40 mesh sieve to obtain refined caulis Akebiae pericarp powder;
q2: adding 0.02-0.05 g of pectinase, 0.02-0.05 g of cellulase and 0.02-0.05 g of amylase into each kilogram of akebia trifoliata peel powder respectively, adding an enzyme activator polyether into the mixture according to the mass ratio of 0.3-0.5% of pectinase, mixed enzyme of cellulase and amylase and the enzyme activator polyether, uniformly mixing the materials, and carrying out enzymolysis in a reaction tank to obtain akebia trifoliata peel enzymatic hydrolysate;
q3: adding 70% methanol into the akebia trifoliata peel enzymatic hydrolysate according to the volume ratio of 1: 6-10, fully stirring and extracting, separating an extracting solution and filter residues, and repeating for 2-3 times;
q4: sequentially adsorbing, washing and eluting the extracting solution, and collecting akebia trifoliata peel powder eluent; concentrating the eluate, and freeze drying to obtain total saponin extract.
2. The method for improving the extraction yield of the total saponins in the akebia trifoliata peels as claimed in claim 1, wherein the slicing and crushing in the step Q1 are carried out by cutting the akebia trifoliata peels into 3-5 mm slices and then crushing the akebia trifoliata peels in a traditional Chinese medicine crusher.
3. The method for improving the extraction yield of the total saponins in the akebia trifoliata peels as claimed in claim 1, wherein the enzymolysis temperature in the step Q2 is 35-55 ℃, the enzymolysis is carried out in a reaction tank for 2-3 h, and then the pressure in the reaction tank is restored to be in a normal pressure state, so that akebia trifoliata peel enzymolysis liquid is obtained.
4. The method for improving the extraction yield of the total saponins in the akebia trifoliata peels as claimed in claim 3, wherein the enzymolysis in the step is sequentially carried out at 35 ℃ for 0.5h, 45 ℃ for 1h and 55 ℃ for 1 h.
5. The method for improving the extraction yield of the total saponins in the akebia trifoliata peels as claimed in claim 3, wherein the pressure in the reaction tank in the enzymolysis process is 0.4-0.6 Mpa, and the pH value is 4-5.
6. The method for improving the extraction rate of the total saponins in the pericarp of akebia trifoliata as claimed in claim 1, wherein the temperature of the methanol added in the step Q3 is 70-90 ℃.
7. The method for improving the extraction yield of the total saponins in the pericarp of akebia trifoliata as claimed in claim 1, wherein the step Q4 is implemented by using a macroporous resin adsorption method for adsorption and enrichment, washing with 40% ethanol and purified water, and then eluting with 95% ethanol solution for concentration to obtain an eluent; adjusting the pH value of the eluent to 2-4, and concentrating and recovering the eluent at 40-60 ℃ for 60-90 min to obtain a concentrated solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010487718.3A CN111494442A (en) | 2020-06-02 | 2020-06-02 | Method for improving extraction yield of total saponins in akebia trifoliata peels |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010487718.3A CN111494442A (en) | 2020-06-02 | 2020-06-02 | Method for improving extraction yield of total saponins in akebia trifoliata peels |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111494442A true CN111494442A (en) | 2020-08-07 |
Family
ID=71849122
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010487718.3A Pending CN111494442A (en) | 2020-06-02 | 2020-06-02 | Method for improving extraction yield of total saponins in akebia trifoliata peels |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111494442A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107260777A (en) * | 2017-06-28 | 2017-10-20 | 南京中医药大学 | A kind of fiveleaf akebia fruit with antitumor activity refines total saposins and its application |
| CN108658919A (en) * | 2018-05-04 | 2018-10-16 | 铜仁学院 | The method for purifying and separating of Akebia trifoliate koiaz Peels flavones |
| CN109966330A (en) * | 2019-05-27 | 2019-07-05 | 江西省药品检验检测研究院 | Active component, the preparation method and application for having diuresis are extracted from threeleaf akebia |
| CN110066841A (en) * | 2019-05-05 | 2019-07-30 | 陕西科技大学 | A method of it improving radix paeoniae rubra effective component albiflorin and extracts yield |
| CN110693930A (en) * | 2019-10-31 | 2020-01-17 | 江苏红豆杉药业有限公司 | Method for extracting high-purity panax notoginseng saponins |
-
2020
- 2020-06-02 CN CN202010487718.3A patent/CN111494442A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107260777A (en) * | 2017-06-28 | 2017-10-20 | 南京中医药大学 | A kind of fiveleaf akebia fruit with antitumor activity refines total saposins and its application |
| CN108658919A (en) * | 2018-05-04 | 2018-10-16 | 铜仁学院 | The method for purifying and separating of Akebia trifoliate koiaz Peels flavones |
| CN110066841A (en) * | 2019-05-05 | 2019-07-30 | 陕西科技大学 | A method of it improving radix paeoniae rubra effective component albiflorin and extracts yield |
| CN109966330A (en) * | 2019-05-27 | 2019-07-05 | 江西省药品检验检测研究院 | Active component, the preparation method and application for having diuresis are extracted from threeleaf akebia |
| CN110693930A (en) * | 2019-10-31 | 2020-01-17 | 江苏红豆杉药业有限公司 | Method for extracting high-purity panax notoginseng saponins |
Non-Patent Citations (6)
| Title |
|---|
| 《中医药发展与现代科学技术》编委会: "《中医药发展与现代科学技术》", 30 September 2005, 四川科学技术出版社 * |
| 张铮等: "三叶木通不同部位有效成分含量比较研究", 《中药材》 * |
| 成晓霞等: "三叶木通中常春藤皂苷的提取工艺", 《安徽农业科学》 * |
| 李医明: "《中药化学》", 31 August 2018, 上海科学技术出版社 * |
| 石兵艳等: "八月瓜果皮中常春藤皂苷元水解工艺优化及图谱分析", 《食品与发酵工业》 * |
| 罗辉等: "贵州三叶木通果皮HPLC指纹图谱初步研究", 《贵州林业科技》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106380501B (en) | A kind of method that stigmasterol is extracted in the stem from Job's tears | |
| CN102106928B (en) | Method for preparing high-purity oil tea saponins | |
| CN112321450B (en) | Preparation method of hydroxyl-alpha-sanshool monomer | |
| CN107721966A (en) | A kind of Suaeda salsa anthocyanidin and biogenic salt combined extraction method | |
| CN111588043B (en) | Preparation method of dietary fiber based on Siraitia grosvenorii waste | |
| CN106349405A (en) | Method for extracting pectin from shaddock peel through enzymolysis and ultrasonic waves | |
| CN110041439B (en) | Method for extracting starch from momordica grosvenori root tuber | |
| CN110194807B (en) | Method for extracting water-soluble active substance of coix seeds | |
| CN113797233A (en) | Novel traditional Chinese medicine decoction piece processing technology based on biological enzymolysis technology | |
| CN114949915A (en) | Hericium erinaceus compound extract and preparation method thereof | |
| CN101935717A (en) | Method for efficiently separating xylose from beer lees | |
| EP1747000B1 (en) | Ethanol extraction of phytosterols from corn fiber | |
| CN103087144A (en) | Diosgenin production method | |
| CN111494442A (en) | Method for improving extraction yield of total saponins in akebia trifoliata peels | |
| CN102002072A (en) | Process for extracting flavone from date pit | |
| CN102127131A (en) | Catalpol extracting method | |
| CN110066841B (en) | Method for improving extraction yield of active ingredient albiflorin of red peony root | |
| CN110903168B (en) | Method for subcritical extraction of solanesol in waste tobacco leaves | |
| CN108606983A (en) | A kind of preparation method of taxus active extract | |
| CN109467580B (en) | Extraction process of naringin | |
| CN110256189B (en) | Process for extracting lycopene from tomato peel residue | |
| CN107412277A (en) | A kind of brown algae polyphenols extracted from sargassum fusifome | |
| CN1508139A (en) | Process for extracting rubusoside of fruit of Gorsvenor Momordica | |
| CN108558645B (en) | Method for extracting crocin from gardenia | |
| CN112812144A (en) | Method for extracting gastrodin extract |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20200807 |
|
| WD01 | Invention patent application deemed withdrawn after publication |