CN111471587A - Case is preserved to microorganism sample - Google Patents
Case is preserved to microorganism sample Download PDFInfo
- Publication number
- CN111471587A CN111471587A CN202010275141.XA CN202010275141A CN111471587A CN 111471587 A CN111471587 A CN 111471587A CN 202010275141 A CN202010275141 A CN 202010275141A CN 111471587 A CN111471587 A CN 111471587A
- Authority
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- China
- Prior art keywords
- case
- pipe
- keeping
- sterile water
- containing pipe
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 244000005700 microbiome Species 0.000 title claims abstract description 34
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 38
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 25
- 239000008223 sterile water Substances 0.000 claims abstract description 23
- 238000007789 sealing Methods 0.000 claims abstract description 16
- 238000005192 partition Methods 0.000 claims description 16
- 239000011521 glass Substances 0.000 claims description 3
- 230000002906 microbiologic effect Effects 0.000 claims 6
- 239000000463 material Substances 0.000 claims 1
- 238000004321 preservation Methods 0.000 abstract description 13
- 241000894006 Bacteria Species 0.000 abstract description 5
- 241000700605 Viruses Species 0.000 description 3
- 230000000694 effects Effects 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 241000195493 Cryptophyta Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 108091005461 Nucleic proteins Proteins 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 241000902900 cellular organisms Species 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/20—Degassing; Venting; Bubble traps
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/04—Seals
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/22—Means for packing or storing viable microorganisms
Abstract
The invention relates to the technical field of microorganism preservation equipment, in particular to a microorganism sample preservation box which comprises a preservation box and a containing pipe, and through the arrangement of the thermostatic board and the ultraviolet disinfection lamp, when the containing pipe is fixed on the clapboard, the ultraviolet disinfection lamp is firstly used, the inside of the sterile water and the storage box is sterilized, the damage of external bacteria to microorganisms is avoided, the temperature of the sterile water is adjusted by the constant temperature plate which is provided with the sterile water and the constant temperature plate, the containing pipe is inserted in the sterile water, so that the water bath of the containing pipe is arranged inside the sterile water, the temperature inside the containing pipe can be more conveniently adjusted, the existence of microorganisms is facilitated, and through being equipped with the sealing member, avoid outside bacterium to enter into the inside of aseptic water through the screw hole, avoid aseptic water to receive the pollution, be more convenient for preserve the microorganism.
Description
Technical Field
The invention relates to the technical field of microorganism preservation equipment, in particular to a microorganism sample preservation box.
Background
The microorganism refers to a general name of all microorganisms difficult to observe by naked eyes, the microorganisms comprise bacteria, viruses, fungi, a few algae and the like, the viruses are non-cellular organisms consisting of a few components such as nucleic acid, protein and the like, but the viruses are divided into space microorganisms, marine microorganisms and the like according to different existing environments, the space microorganisms, the marine microorganisms and the like are divided into prokaryotic microorganisms and eukaryotic microorganisms according to cell structure classification, when the microorganisms are researched, a research object needs to be sampled, and a part of individuals actually observed or researched in the research is called as samples;
when the sample of microorganism is preserved, because the environment that the existence of microorganism needs is different, need use different case of keeping, and prior art adopts the refrigerator to store mostly, causes easily when storing and destroys the microorganism, influences the research, is unfavorable for the investigation.
Disclosure of Invention
The present invention is directed to a case for storing a microorganism specimen, which solves the problems of the prior art that it is inconvenient to store the specimen and the specimen is easily damaged during storage.
In order to achieve the purpose, the invention provides the following technical scheme: comprises a storage box and a holding pipe, wherein one side of the outer surface of the storage box is fixedly provided with a power plug, one side of the upper surface of the storage box is hinged with a box cover, the lower surface in the storage box is fixedly provided with a thermostatic board, ultraviolet disinfection lamps are fixedly arranged on both sides in the storage box, a temperature control switch and a disinfection switch are respectively and fixedly arranged in the middle of one side of the outer surface of the storage box, the power plug is electrically connected with the temperature control switch and the disinfection switch, the temperature control switch is electrically connected with the thermostatic board, the disinfection switch is electrically connected with the ultraviolet disinfection lamp, a clapboard is fixedly arranged in the storage box, the equal threaded connection of upper surface of baffle has the sealing member, hold pipe threaded connection on the baffle, the inside of keeping the case is located the lower surface injection of baffle has aseptic water.
Preferably, a waterproof cover is fixedly arranged on the outer side of the ultraviolet disinfection lamp.
Preferably, threaded holes are evenly formed in the lower surface of the box cover and the partition plate, the sealing element is in threaded connection with the inner portion of the threaded holes, and a sealing gasket is fixedly mounted at one end of the connecting piece and one end of the containing pipe.
Preferably, the holding pipe is in threaded connection with the threaded hole in the partition plate, a vent hole is formed in the upper end of the holding pipe, and the lower end of the holding pipe extends to the interior of the sterile water.
Preferably, the lower fixed surface of the storage box is provided with a support frame, one side of the storage box is fixedly provided with a water inlet pipe, and the water inlet pipe is communicated with the interior of the storage box.
Preferably, one side of the upper end of the storage box is uniformly provided with air inlet holes.
Preferably, the containing pipe is made of transparent glass.
The invention has the technical effects and advantages that:
1. according to the invention, the constant temperature plate and the ultraviolet disinfection lamp are arranged, when the containing pipe is fixed on the partition plate, the ultraviolet disinfection lamp is firstly used for sterilizing the sterile water and the inside of the storage box, so that the external bacteria are prevented from damaging microorganisms and influencing samples;
2. according to the invention, the sterile water is arranged, the constant temperature plate is arranged, the temperature of the sterile water is regulated through the constant temperature plate, the containing pipe is inserted in the sterile water, so that the containing pipe is bathed in the sterile water, the temperature in the containing pipe is more conveniently regulated, the existence of microorganisms is facilitated, the sealing element is arranged, external bacteria are prevented from entering the sterile water through the threaded hole, the sterile water is prevented from being polluted, and the microorganisms are more conveniently stored.
Drawings
Fig. 1 is a front sectional view of the present invention.
Fig. 2 is a front view of the present invention.
FIG. 3 is a schematic view showing the structure of the holding tube of the present invention.
Fig. 4 is a schematic structural view of the separator of the present invention.
Fig. 5 is a schematic structural view of the sealing member of the present invention.
FIG. 6 is a diagram of electrical connection according to the present invention.
In the figure: 1. a storage box; 2. a holding tube; 3. a power plug; 4. a box cover; 5. a thermostatic plate; 6. an ultraviolet disinfection lamp; 7. a temperature control switch; 8. a sterilizing switch; 9. a partition plate; 10. a seal member; 11. sterile water; 12. a waterproof cover; 13. a threaded hole; 14. a gasket; 15. a vent hole; 16. a support frame; 17. a water inlet pipe; 18. and (4) air inlet holes.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention provides a microorganism sample storage box as shown in figures 1-6, which comprises a storage box 1 and a storage tube 2, wherein the storage tube 2 is made of transparent glass, a support frame 16 is fixedly arranged on the lower surface of the storage box 1, one side of the upper end of the storage box 1 is uniformly provided with an air inlet 18, one side of the storage box 1 is fixedly provided with a water inlet tube 17, the water inlet tube 17 is communicated with the interior of the storage box 1, one side of the outer surface of the storage box 1 is fixedly provided with a power plug 3, one side of the upper surface of the storage box 1 is hinged with a box cover 4, the lower surface of the interior of the storage box 1 is fixedly provided with a constant temperature plate 5, two sides of the interior of the storage box 1 are fixedly provided with ultraviolet disinfection lamps 6, the outer side of the ultraviolet disinfection lamps 6 is fixedly provided with a waterproof cover 12, the middle position of one side of the outer surface of the storage box 1 is, the power plug 3 is electrically connected with a temperature control switch 7 and a disinfection switch 8, the temperature control switch 7 is electrically connected with a thermostatic board 5, the disinfection switch 8 is electrically connected with an ultraviolet disinfection lamp 6, a partition board 9 is fixedly installed inside the preservation box 1, the upper surface of the partition board 9 is in threaded connection with a sealing piece 10, the holding pipe 2 is in threaded connection with the partition board 9, and sterile water 11 is injected into the preservation box 1, is positioned on the lower surface of the partition board 9;
evenly set up threaded hole 13 on the lower surface of case lid 4 and the baffle 9, sealing member 10 threaded connection is in the inside of threaded hole 13, and the equal fixed mounting of connecting member 10 and the one end that holds pipe 2 has sealed the pad 14, holds 2 threaded connection of pipe in the threaded hole 13 on the baffle 9, and the upper end that holds pipe 2 is equipped with air vent 15, holds the inside that the lower extreme of pipe 2 extends to aseptic water 11.
As shown in fig. 3 and 5, sealing gaskets 14 are provided at both the lower end of the containing pipe 2 and the lower end of the sealing member 10, so that when the containing pipe 2 and the sealing member 10 are screwed inside the screw hole 13 formed in the partition plate 9, the sterile water 11 is prevented from flowing into the upper end of the partition plate 9 through the screw hole 13, and the sealing effect is improved.
This practical theory of operation: when the invention is used, the power plug 3 is electrically connected with an external commercial power, the sterile water 11 is injected into the preservation box 1 through the water inlet pipe 17, the thermostatic board 5 and the ultraviolet disinfection lamp 6 are started through the temperature control switch 7 and the disinfection switch 8, the ultraviolet disinfection lamp 6 sterilizes the sterile water 11 and the preservation box 1, the thermostatic board 5 adjusts the temperature of the sterile water 11, so that the temperature of the sterile water 11 is suitable for the growth of microorganisms, a sample of the microorganisms is placed in the preservation pipe 2, the box cover 4 is opened, the sealing element 10 on the partition board 9 is screwed down and screwed into the threaded hole 13 of the box cover 4, the sealing element 10 is convenient to be accommodated, the preservation pipe 2 is inserted into the threaded hole 13 on the partition board 9 and is in threaded connection with the threaded hole 13, so that the lower end of the preservation pipe 2 extends into the sterile water 11, and the preservation pipe 2 is fixed in the threaded hole 13 on the partition board 9, and be equipped with sealed 14 at the lower extreme that holds pipe 2 and seal up, avoid aseptic water 11 to enter into the upper end of baffle 9 through screw hole 13, make the inside at aseptic water 11 of holding pipe 2 water bath that holds the microorganism, be convenient for carry out stable regulation to the temperature that holds 2 insides of pipe more, be convenient for the existence of microorganism more, and be equipped with inlet port 18 in one side of case 1, upper end holding pipe 2 is equipped with air vent 15, be convenient for oxygen enters into the inside of case 1, and then be convenient for the existence of microorganism, be convenient for preserve the microorganism, and convenient to use.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments or portions thereof without departing from the spirit and scope of the invention.
Claims (7)
1. The utility model provides a case is preserved to microorganism sample, includes and preserves case (1) and hold pipe (2), its characterized in that: the utility model discloses a solar energy water heater, including the storage box (1), the surface one side fixed mounting who keeps the case (1) has power plug (3), the upper surface one side of keeping the case (1) articulates there is case lid (4), the inside lower fixed surface of keeping the case (1) installs thermostated plate (5), the equal fixed mounting in inside both sides of keeping the case (1) has ultraviolet ray disinfection lamp (6), the intermediate position of the surface one side of keeping the case (1) fixed mounting respectively has temperature detect switch (7) and disinfection switch (8), power plug (3) electric connection temperature detect switch (7) with disinfection switch (8), temperature detect switch (7) electric connection thermostated plate (5), disinfection switch (8) electric connection ultraviolet ray disinfection lamp (6), the inside fixed mounting of keeping the case (1) has baffle (9), the equal threaded connection in upper surface of baffle (9) has sealing member (10), the containing pipe (2) is in threaded connection with the partition plate (9), and sterile water (11) is injected into the lower surface of the partition plate (9) in the storage box (1).
2. A microbiological specimen storage container according to claim 1, wherein: and a waterproof cover (12) is fixedly arranged on the outer side of the ultraviolet disinfection lamp (6).
3. A microbiological specimen storage container according to claim 1, wherein: the lower surface of case lid (4) with evenly set up threaded hole (13) on baffle (9), sealing member (10) threaded connection be in the inside of threaded hole (13), just connecting piece (10) with the equal fixed mounting in one end of holding pipe (2) has sealed pad (14).
4. A microbiological specimen storage container according to claim 4, wherein: the containing pipe (2) is in threaded connection with the partition plate (9) in the threaded hole (13), a vent hole (15) is formed in the upper end of the containing pipe (2), and the lower end of the containing pipe (2) extends to the inside of the sterile water (11).
5. A microbiological specimen storage container according to claim 1, wherein: the lower fixed surface of keeping case (1) installs support frame (16), one side fixed mounting who keeps case (1) has inlet tube (17), inlet tube (17) with the inside of keeping case (1) link up mutually.
6. A microbiological specimen storage container according to claim 1, wherein: one side of the upper end of the storage box (1) is uniformly provided with air inlet holes (18).
7. A microbiological specimen storage container according to claim 1, wherein: the holding pipe (2) is made of transparent glass materials.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN202010275141.XA CN111471587A (en) | 2020-04-09 | 2020-04-09 | Case is preserved to microorganism sample |
Applications Claiming Priority (1)
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CN202010275141.XA CN111471587A (en) | 2020-04-09 | 2020-04-09 | Case is preserved to microorganism sample |
Publications (1)
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CN111471587A true CN111471587A (en) | 2020-07-31 |
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CN202010275141.XA Pending CN111471587A (en) | 2020-04-09 | 2020-04-09 | Case is preserved to microorganism sample |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN115318807A (en) * | 2022-08-11 | 2022-11-11 | 中建安装集团有限公司 | Kitchen waste treatment device adopting technical microbial decomposition |
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US20040096961A1 (en) * | 2002-11-15 | 2004-05-20 | Yunping Huang | High temperature incubation system and method for small volumes |
JP6228282B1 (en) * | 2016-09-27 | 2017-11-08 | 株式会社協和医療器 | Bacteria culture inspection device and bacteria culture inspection method |
CN207791529U (en) * | 2017-12-04 | 2018-08-31 | 江苏安舜技术服务有限公司 | A kind of portable food detection Sample storage case |
CN208121083U (en) * | 2018-03-27 | 2018-11-20 | 天津康洋基因科技有限公司 | A kind of oral cavity flora cultivate reagent box |
CN109187965A (en) * | 2018-09-14 | 2019-01-11 | 重庆金域医学检验所有限公司 | HPV detection kit |
CN208532750U (en) * | 2018-03-26 | 2019-02-22 | 天津康洋基因科技有限公司 | A kind of intestinal flora cultivate reagent box |
CN208542233U (en) * | 2018-02-27 | 2019-02-26 | 大连京诚盛宏源检测技术有限公司 | A kind of oxygen barrier sterilizing water-bath |
CN110605151A (en) * | 2018-06-15 | 2019-12-24 | 四川聚泉生物科技有限公司 | Test tube heating heat preservation device for biotechnology |
-
2020
- 2020-04-09 CN CN202010275141.XA patent/CN111471587A/en active Pending
Patent Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20040096961A1 (en) * | 2002-11-15 | 2004-05-20 | Yunping Huang | High temperature incubation system and method for small volumes |
JP6228282B1 (en) * | 2016-09-27 | 2017-11-08 | 株式会社協和医療器 | Bacteria culture inspection device and bacteria culture inspection method |
CN207791529U (en) * | 2017-12-04 | 2018-08-31 | 江苏安舜技术服务有限公司 | A kind of portable food detection Sample storage case |
CN208542233U (en) * | 2018-02-27 | 2019-02-26 | 大连京诚盛宏源检测技术有限公司 | A kind of oxygen barrier sterilizing water-bath |
CN208532750U (en) * | 2018-03-26 | 2019-02-22 | 天津康洋基因科技有限公司 | A kind of intestinal flora cultivate reagent box |
CN208121083U (en) * | 2018-03-27 | 2018-11-20 | 天津康洋基因科技有限公司 | A kind of oral cavity flora cultivate reagent box |
CN110605151A (en) * | 2018-06-15 | 2019-12-24 | 四川聚泉生物科技有限公司 | Test tube heating heat preservation device for biotechnology |
CN109187965A (en) * | 2018-09-14 | 2019-01-11 | 重庆金域医学检验所有限公司 | HPV detection kit |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN115318807A (en) * | 2022-08-11 | 2022-11-11 | 中建安装集团有限公司 | Kitchen waste treatment device adopting technical microbial decomposition |
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Application publication date: 20200731 |