CN111450230A

CN111450230A - Application of corn oligopeptide in intervention of myocardial injury

Info

Publication number: CN111450230A
Application number: CN202010512185.XA
Authority: CN
Inventors: 陈宁; 陶景景; 吴良文; 刘天彧
Original assignee: Wuhan Sports University
Current assignee: Wuhan Sports University
Priority date: 2020-06-08
Filing date: 2020-06-08
Publication date: 2020-07-28

Abstract

The invention discloses application of corn oligopeptide in intervention of myocardial injury, and widens the application field of the corn oligopeptide.

Description

Application of corn oligopeptide in intervention of myocardial injury

Technical Field

The invention relates to the technical field of food and medicine, in particular to application of corn oligopeptide in intervention of myocardial injury.

Background

The corn oligopeptide is a small molecular peptide obtained by performing enzymolysis reaction on corn protein. The existing data report that the corn oligopeptide has the effects of sobering up, resisting apoptosis, resisting fatigue, resisting oxidation, inhibiting tumor growth and the like.

The heart function can be obviously improved and strengthened by moderate exercise, the heart function can be damaged by exhaustive exercise, and the heart bearing capacity is exceeded by the excessive load of the exhaustive exercise, so that a series of pathological changes or organic changes occur to the heart. Research shows that the mitochondrial structure is obviously damaged immediately after heavy-load exercise, and the myofibrillar structure is obviously damaged 24h after exercise. The specific manifestations are abnormal muscle segments, disorderly arrangement of myofilaments, incomplete dispersion of myofibrils, and possibly phenomena of partial myofibril breakage, change of floccule, fuzzy nuclear membrane and chromatin edge collection. Prolonged exhaustive exercise can also lead to ischemic and hypoxic injury or necrosis of myocardial cells, and the manifestation of myocardial injury such as arrhythmia, myocardial hypertrophy or myocardial functional structural abnormality.

Researchers construct a model of disposable large-load exhaustion exercise heart injury through animals, supplement various nutritional supplements or traditional Chinese medicines during or before experiments, observe and analyze the protective effect of the medicines on heart structure and function injury of disposable exhaustion exercise mice. The research personnel find that the nutritional agent or the traditional Chinese medicine can improve the myocardial structure and function damage caused by the exhaustive exercise and simultaneously has the function of protecting the mitochondrial structure and function of myocardial cells by supplementing the nutritional agent or the traditional Chinese medicine before or after the exhaustive exercise. Among the supplements available are: astragalus polysaccharide, vitamin E and total soyasaponin.

Disclosure of Invention

In order to solve the problems, the invention provides the application of the corn oligopeptide in the intervention of myocardial injury.

The invention discloses application of corn oligopeptide in intervention of myocardial injury.

Preferably, the application of the corn oligopeptide comprises one or the combination of the following: reducing the apoptosis rate of the myocardial cells, improving the autophagy level of the myocardial cells and improving the exercise capacity.

Preferably, the application of the corn oligopeptide comprises one or the combination of the following: regulating the expression of anti-apoptosis related protein of the myocardial cells, regulating the level of autophagy related protein of the myocardial cells, reducing the content of creatine kinase and isozyme thereof in serum, improving the level of anti-oxidation related protein of the myocardial cells, improving the activity of superoxide dismutase of the serum and reducing the content of malondialdehyde.

Preferably, the corn oligopeptide is also combined with exercise training.

Preferably, the method for corn oligopeptide combined exercise training comprises the following steps: exercise training was performed during the period of feeding with the corn oligopeptide.

Preferably, the preparation method of the corn oligopeptide comprises the following steps: obtaining corn protein; adding water into corn protein and then carrying out ultrasonic pretreatment; after pretreatment, uniformly stirring the zein suspension; enzymolysis of zein in suspension by alkaline protease; centrifuging the suspension after enzymolysis, and taking the supernatant; filtering the supernatant to obtain a filtrate; sterilizing the filtrate at high temperature for 3 seconds; and drying the sterilized filtrate.

Preferably, the alkaline protease comprises bacillus licheniformis protease, the addition amount of the alkaline protease is 0.5-1.5% of the weight of the zein, the enzymolysis condition is 58 ℃, the pH value is 8.8, and the enzymolysis time is 4 hours.

Preferably, the corn oligopeptide is used for preparing food or medicines.

Preferably, the food comprises a solid or liquid beverage, the content of the corn oligopeptide in the liquid beverage is 1-2g/100ml, and the weight percentage of the corn oligopeptide in the solid beverage is 30-70%.

Preferably, the corn oligopeptide or the exercise training and corn oligopeptide is applied to the intervention of the exercise-induced myocardial injury.

Preferably, the corn oligopeptide or the exercise training and corn oligopeptide is applied to intervene in myocardial damage caused by excessive exercise or excessive fatigue.

Compared with the prior art, the invention has the beneficial effects that:

1. the invention widens the application field of the corn oligopeptide, and the corn oligopeptide is applied to intervention myocardial injury for the first time; 2. the corn oligopeptide or the corn oligopeptide combined with the exercise training is used for reducing the content of creatine kinase and isozyme thereof in serum, reducing the apoptosis rate of myocardial cells, improving the autophagy level of the myocardial cells, regulating the level of autophagy-related protein of the myocardial cells, improving the level of oxidation-related protein of the myocardial cells, improving the activity of superoxide dismutase of the serum or reducing the content of malondialdehyde.

Drawings

FIG. 1 is an experimental flow chart of the present invention;

FIG. 2 is a graph of the trend of blood lactate values over time;

FIG. 3 is a graph comparing the difference between blood lactate after a depletion experiment and blood lactate at rest;

FIG. 4 is a bar graph of creatine kinase activity in serum;

FIG. 5 is a bar graph of creatine kinase isoenzyme content in serum;

FIG. 6 is a bar graph of the rate of TUNE L positive cells;

FIG. 7 is a Western blot comparison of Bcl-2, Bax and GAPDH;

FIG. 8 is a bar graph of the relative expression level of Bcl-2;

FIG. 9 is a bar graph of relative Bax expression;

FIG. 10 is a bar graph of the ratio of the relative expression levels of Bcl-2 and Bax;

FIG. 11 is a Western blot comparison of Atg7, Beclin-1, p62, L C3-I, L C3-II and GAPDH;

FIG. 12 is a bar graph of the relative expression of Atg 7;

FIG. 13 is a bar graph of the relative content of Beclin-1;

FIG. 14 is a histogram of the relative content of p 62;

FIG. 15 is a bar graph of the relative expression ratio of L C3-II to L C3-I;

FIG. 16 is a Western blot comparison of Nrf2, Keap1 and GAPDH;

FIG. 17 is a bar graph of the relative expression of Nrf 2;

FIG. 18 is a histogram of relative expression of Keap 1;

FIG. 19 is a histogram of serum SOD activity;

FIG. 20 is a histogram of serum MDA content;

FIG. 21 is a flow diagram of a method for preparing corn oligopeptides.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.

The invention is described in further detail below with reference to the attached drawing figures:

in order to study the effect of corn oligopeptide on preventing myocardial damage, as shown in fig. 1, the following detection experiment is constructed in the invention.

Step 101: 40 mice were obtained, which were SPF grade ICR mice weighing about 40 g. The experimental mice are purchased from the research center of experimental animals in northern lake province, and the qualification numbers of the mice are as follows: (NO. 42000600023933).

102, carrying out adaptive feeding for 1 week, dividing the adaptive swimming group into 5 groups, namely a quiet control group (NC group), a swimming training control group (SC group), a corn oligopeptide low-dose combined swimming training group (S L group), a corn oligopeptide medium-dose combined swimming training group (SM group) and a corn oligopeptide high-dose combined swimming training group (SH group), wherein 8 animals are fed in each group after 3 days of adaptive swimming.

103, infusing equal volume of physiological saline into the stomach every day by an NC group, infusing equal volume of physiological saline into the stomach every day by an SC group, carrying out swimming training 5 days every week, infusing 0.2g/kg of corn oligopeptide every day by an S L group, carrying out swimming training 5 days every week, infusing 0.4g/kg of corn oligopeptide every day by an SM group, carrying out swimming training 5 days every week, infusing 0.8g/kg of corn oligopeptide every day by an SH group, carrying out swimming training 5 days every week, wherein the volume of the infused stomach of each group every day is the same, the swimming training time is gradually prolonged from 30min to 45min, and the NC group does not carry out swimming training during which each mouse keeps freely eating and drinking water.

Step 104: after eight weeks, each group was individually tested for load exhaustion. The mouse load was 5% of its body weight, exhaustive swimming time was recorded separately, and blood lactate levels were measured before exhaustive exercise, immediately after exhaustive exercise, 5 minutes after exhaustive exercise, 15 minutes after exhaustive exercise, and 30 minutes after exhaustive exercise.

The lead sheath is used as a weight and fixed at the tail part, when the mouse is immersed in water for more than 10s, the mouse still can not return to the water surface independently, and the swimming of the mouse is extremely uncoordinated, the mouse is immediately fished out of the water, the turning over experiment can not be carried out as a judgment standard, and the judgment of exhaustion is finished if the mouse and the turning over experiment meet the judgment standard at the same time.

Step 105: blood and myocardium are obtained. After the exhaustive exercise experiment is finished, the eyeball takes blood within 24 hours, then the neck is removed, the mouse is killed, and the myocardial tissue of the mouse is collected. The myocardial tissue is used to harvest myocardial cells.

And 106, respectively detecting the apoptosis rate of the myocardial cells, detecting related proteins and analyzing biochemical indexes of blood, wherein the apoptosis rate of the myocardial cells is detected by a TUNE L fluorescence staining method, and specifically, a TUNE L apoptosis detection kit is adopted, the related proteins comprise Atg7, Beclin-1, p62, L C3-II, L C3-I, Nrf2, Keap1, Bax and Bcl-2, the related proteins are detected by a Western blot method, the relative gray values of all bands are counted based on Image J, and the relative expression quantity is calculated by taking GAPDH as reference, the biochemical indexes of blood comprise the detection of SOD, MDA, CK and CK-MB, wherein the SOD is detected by a superoxide dismutase kit, the MDA is detected by a malondialdehyde kit, the CK activity is detected by a CK kit, and the CK-MB is detected by a kinase isozyme kit.

The experimental results and analysis are as follows:

the corn oligopeptide can be used for reducing the content of creatine kinase and isozyme in serum, and the experimental results are shown in the following table.

The experimental data of the invention are expressed by mean value plus or minus standard deviation (M plus or minus SD), the significant difference is detected by independent sample T, the difference is obvious when p is less than 0.05, and the difference is obvious when p is less than 0.01. Symbolic illustration in the figures or tables: compared with the NC group, p <0.05, i.e. the difference is significant, p <0.01, i.e. the difference is significant; compared with the SC group, # means p <0.05, i.e. significant difference, # means p <0.01, i.e. significant difference; the two ends of the horizontal line in the figure point to the two comparison groups.

CK is creatine kinase, mainly present in the cytoplasm and mitochondria, and is an important kinase directly involved in intracellular energy transfer, muscle contraction, and ATP regeneration. CK-MB is an isozyme of creatine kinase. When the exercise is done, harmful substances in the cardiac muscle cells are accumulated, and ischemia and hypoxia cause cardiac muscle cell damage, so that enzymes in the cardiac muscle cells enter blood, and therefore, CK and CK-MB are used as markers of cardiac muscle damage.

As shown in fig. 4, serum CK activity was decreased in SC, S L, SM, and SH groups compared to NC group, in which serum CK activity was significantly decreased in S L group, and SM group and SH serum CK activity were significantly decreased in SM group, and SH group, in which serum CK activity was significantly decreased in S L, SM, and SH group compared to SC group, and in S L group.

As shown in FIG. 5, the CK-MB contents of the sera of the SC, S L, SM and SH groups were reduced compared with the NC group, wherein the CK-MB content of the sera of the S L group was significantly reduced, and the CK-MB contents of the sera of the S L, SM and SH groups were reduced compared with the SC group.

Therefore, the intervention of the corn oligopeptide or the exercise training and the corn oligopeptide can reduce the CK and CK-MB level and play a role in myocardial preservation, wherein the effect of the low-dose corn oligopeptide and swimming training is most obvious.

Second, the maize oligopeptide can be used to reduce the rate of cardiomyocyte apoptosis, as shown in the following table.

As shown in fig. 6, the unit cell rate positive to the ordinate TUNE L in the graph represents the cardiomyocyte apoptosis rate, and the cardiomyocyte apoptosis rates of the SC group, S L group, SM group and SH group were decreased compared to the NC group, wherein the cardiomyocyte apoptosis rate of the S L group was significantly decreased and the cardiomyocyte apoptosis rate of the SH group was significantly decreased, and the cardiomyocyte apoptosis rates of the S L group, SM group and SH group were decreased compared to the SC group, wherein the cardiomyocyte apoptosis rate of the SH group was significantly decreased.

Therefore, the intervention mode of the corn oligopeptide or the movement combined corn oligopeptide can effectively reduce the apoptosis rate in the exhausted motor central muscle tissue.

And thirdly, the corn oligopeptide can be used for expressing the anti-apoptosis protein of the upper central myocyte.

Bcl-2 is an anti-apoptotic protein; bax is an apoptosis protein and can form heterodimer with Bcl-2 to generate suppression effect on the Bcl-2.

FIG. 7 is a Western blot comparison of Bcl-2, Bax and GAPDH, and FIGS. 8 to 10 calculate the relative expression level based on the Image J statistics of the relative intensity of each band in FIG. 7 and GAPDH as a reference.

As shown in FIGS. 7-10, the relative expression levels of Bcl-2 of SC, S L, SM and SH groups are increased compared with the NC group, wherein the relative expression level of Bcl-2 of S L group is significantly increased, the relative expression levels of Bax of SC, S L, SM and SH groups are decreased, wherein the relative expression levels of Bax of SM and SH groups are significantly decreased, the relative expression level ratio of Bcl-2 of SC, S L, SM and SH groups to Bax is increased, wherein the relative expression level ratio of SM group is significantly increased, and the relative expression levels of S L and SH groups are significantly increased, and the relative expression level ratio of Bcl-2 of S L group to Bax is significantly increased compared with the SC group, the Bcl-2 of SC, S L, SM and SH groups is decreased, and the relative expression level ratio of Bcl-2 of SC, S L, SM and SH groups to Bax is increased.

Therefore, the corn oligopeptide or the exercise training and the corn oligopeptide can up-regulate the expression of the anti-apoptosis protein of the myocardial cells, reduce the expression of the apoptosis protein and play a role in regulating the expression of the anti-apoptosis related protein, thereby increasing the anti-apoptosis capacity of the myocardial cells.

And fourthly, the corn oligopeptide can be used for improving the autophagy level of the cardiac muscle cells.

The autophagy-related proteins are Beclin-1, Atg7, p62, L C3-II and L C3-I, wherein the Beclin-1 and Atg7 can induce autophagy, the p62 is a marker for autophagy activation, and the ratio of L C3-II to L C3-I is large and small for evaluating the level of autophagy.

As shown in FIGS. 11-15, the relative expression amounts of Atg7 in the cardiomyocytes in the SC, S L and SM groups, Beclin-1 in the cardiomyocytes in the S L, SM and SH groups, and p62 in the cardiomyocytes in the SC, S L, SM and SH groups were significantly increased, wherein the relative expression amounts of S L1 and SH group p62 were significantly decreased, the relative expression amounts of L C3-II and L C3-I in the cardiomyocytes in the SC, S L, SM and SH groups were significantly increased, and the ratio of the relative expression amounts of S L and SH groups was significantly increased, compared with the SC group, the relative expression amounts of Beclin-1 in the S L and SM groups, the relative expression amounts of p62 in the S L, SM and SH groups were decreased, and the relative expression amounts of C3-II and L C3-I in the S L group were significantly increased, and the ratio of C L-C8672 and 36874 in the SH groups was significantly increased.

Thus, the maize oligopeptide can increase the level of myocardial autophagy by modulating the level of the protein associated with myocardial autophagy. The protection of cardiac muscle is achieved by autophagy mechanism.

And fifthly, the corn oligopeptide can be used for improving the level of the antioxidant related protein of the myocardial cells.

The invention detects antioxidant related proteins Nrf2 and Keap 1. Nrf2 is an important transcription factor for regulating and controlling cell oxidative stress response, and can reduce cell damage caused by active oxygen and an electrophile, so that cells are in a stable state, and the redox dynamic balance of an organism is maintained; keap1 is a cytoplasmic binding protein for Nrf 2. Nrf2 normally binds to Keap1 and Nrf2 dissociates from Keap1 under oxidative stress conditions to activate Nrf 2.

As shown in FIGS. 16-18, the relative expression levels of Nrf2 for SC, S L, SM and SH were increased compared to the NC group, where S L and SH were significantly increased, and the relative expression levels of Keap1 for SC, S L, SM and SH, where S L and SH were significantly increased, and the relative expression levels of Nrf2 for S L and SH were increased compared to the SC group, and the relative expression levels of Keap1 for S L, SM and SH were increased, where the relative expression level of Keap1 for S L group was significantly increased.

Therefore, the exercise training or the corn oligopeptide combined exercise training can improve the expression level of the antioxidant protein of the myocardial cells, thereby improving the antioxidant capacity of the myocardial cells.

And sixthly, the corn oligopeptide can be used for improving the serum SOD activity and reducing the MDA content.

Malondialdehyde (MDA) is a myocardial lipid oxidation product, can cause cross-linking polymerization of protein, nucleic acid and other life macromolecules, and has cytotoxicity; superoxide dismutase (SOD) catalyzes superoxide anion free radical disproportionation to generate oxygen and hydrogen peroxide, and plays a vital role in the balance of organism oxidation and antioxidation. The experimental data are shown in the following table:

as shown in fig. 19 and 20, serum SOD activities of SC, S L, SM and SH groups were increased, wherein serum SOD activity of S L group was significantly increased, MDA of SC, S L, SM and SH groups was decreased, wherein MDA of SC and SM group was significantly decreased, and MDA of S L group was significantly decreased, compared to NC group.

Therefore, the corn oligopeptide is combined with exercise training to improve the activity of serum SOD in exhausted exercise and reduce the content of MDA.

Seventhly, the corn oligopeptide prolongs the mouse exhaustion swimming time, and the following table is shown:

as shown in the table, the exhaustion swimming times of the SC, S L, SM and SH groups were improved compared to the NC group, in which the exhaustion swimming time of the S L group was significantly improved, and the exhaustion swimming times of the SM and SH groups were significantly improved, and the exhaustion swimming times of the S L, SM and SH groups were improved compared to the SC group, in which the exhaustion swimming times of the SM and SH groups were significantly improved.

Application of corn oligopeptide in promoting serum lactic acid elimination

As shown in FIG. 2 and FIG. 3, the SC group has a significant increase in lactic acid, the values of lactic acid in the S L, SM and SH groups are lower than those in the NC group, and 30 minutes recover the values from S L, SH, SM, NC and SC. in the order from fast to slow, so that the corn oligopeptide can inhibit the accumulation of lactic acid and promote the clearance of blood lactic acid.

From the above analysis it follows that: the corn oligopeptide or the exercise training and the corn oligopeptide can improve the exercise capacity and intervene in myocardial damage. The corn oligopeptide or the exercise training and the corn oligopeptide can intervene in the exercise myocardial damage. The corn oligopeptide or the exercise training and the corn oligopeptide can intervene in myocardial damage caused by excessive exercise or excessive fatigue.

A method of athletic training in combination with corn oligopeptides may comprise: exercise training was performed during the period of feeding with the corn oligopeptide. The specific exercise training method comprises the following steps: performing adaptive training for 3-7 days under low intensity; the training time is gradually lengthened and/or the training intensity is gradually strengthened. In one embodiment, 10-40g of corn oligopeptide is taken daily while continuing to train daily.

Wherein, the corn oligopeptide can be purchased in the market and also can be prepared in a laboratory or a factory building. As shown in fig. 21, the preparation method of the corn oligopeptide comprises:

step 201: and (5) obtaining the corn protein.

Step 202: the zein is pretreated by ultrasonic wave after being added with water. The ultrasonic wave is used for scattering protein particles and improving the solubility of the zein.

Step 203: after pretreatment, the zein suspension was stirred well.

Step 204: the zein in the suspension is enzymatically hydrolyzed by alkaline protease.

Step 205: and centrifuging the suspension after enzymolysis, and taking the supernatant. After enzymolysis, the corn oligopeptide is dissolved in the supernatant.

Step 206: and filtering the supernatant to obtain a filtrate. Large particles were removed by filtration.

Step 207: the filtrate was sterilized at high temperature for 3 seconds. To improve the stability of the finished product. The sterilization temperature may be 130-135 degrees.

Step 208: and drying the sterilized filtrate. The corn oligopeptide solid can be obtained after drying, and the preservation is facilitated; or the corn oligopeptide solution with high concentration can be obtained.

In step 204, the method for proteolysis of maize comprises: carrying out enzymolysis by using Bacillus licheniformis protease (alcalase), wherein the addition amount of the protease is 0.5-1.5% of the weight of the zein, the enzymolysis condition is 58 ℃, the pH value is 8.8, and the enzymolysis time is 4 hours. The ratio of protease to zein is preferably 1%.

The corn oligopeptide can be used for preparing food or medicines. The food or drug can be absorbed via the stomach by eating. The corn oligopeptide can be used as a main component and can also be used as an auxiliary material. The food product comprises a solid or liquid beverage. The content of the corn oligopeptide in the liquid beverage is 1-2g/100 ml; the weight percentage of the corn oligopeptide in the solid beverage is 30-70%, preferably 33.33-66.67%. The content of the corn oligopeptide in the liquid medicine is 1-2g/100 ml; the weight percentage of the corn oligopeptide in the solid medicine is 30-70%, preferably 33.33-66.67%. The food or medicine can be bagged steeping agent, brewing agent, granules, capsules or oral liquid.

The above is only a preferred embodiment of the present invention, and is not intended to limit the present invention, and various modifications and changes will occur to those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. The application of corn oligopeptide in intervening myocardial injury.

2. The use of a maize oligopeptide according to claim 1, for the intervention of myocardial injury, wherein the use of the maize oligopeptide comprises one or a combination of the following:

reducing the apoptosis rate of the myocardial cells, improving the autophagy level of the myocardial cells and improving the exercise capacity.

3. The use of a maize oligopeptide according to claim 1, for the intervention of myocardial injury, wherein the use of the maize oligopeptide comprises one or a combination of the following: regulating the expression of anti-apoptosis related protein of the myocardial cells, regulating the level of autophagy related protein of the myocardial cells, reducing the content of creatine kinase and isozyme thereof in serum, improving the level of anti-oxidation related protein of the myocardial cells, improving the activity of superoxide dismutase of the serum and reducing the content of malondialdehyde.

4. The use of a maize oligopeptide according to claim 2 or 3, wherein the maize oligopeptide is further combined with exercise training for the intervention of myocardial injury.

5. The use of a corn oligopeptide according to claim 4 for the intervention of myocardial injury, wherein the method of corn oligopeptide in combination with exercise training comprises:

exercise training was performed during the period of feeding with the corn oligopeptide.

6. The use of the corn oligopeptide according to claim 1 for intervening myocardial injury, wherein the preparation method of the corn oligopeptide comprises the following steps:

obtaining corn protein;

adding water into corn protein and then carrying out ultrasonic pretreatment;

after pretreatment, uniformly stirring the zein suspension;

enzymolysis of zein in suspension by alkaline protease;

centrifuging the suspension after enzymolysis, and taking the supernatant;

filtering the supernatant to obtain a filtrate;

sterilizing the filtrate at high temperature for 3 seconds;

and drying the sterilized filtrate.

7. The use of the corn oligopeptide for intervening myocardial injury according to claim 6, wherein the alkaline protease comprises bacillus licheniformis protease, the addition amount of the alkaline protease is 0.5-1.5% of the weight of the corn protein, the enzymolysis condition is 58 ℃, the pH value is 8.8, and the enzymolysis time is 4 hours.

8. Use of a maize oligopeptide according to any one of claims 1 to 3, for the intervention in myocardial injury, wherein the maize oligopeptide is used for the preparation of a food or a medicament.

9. The use of a maize oligopeptide according to claim 8 for the intervention of myocardial injury,

the food comprises a solid or liquid beverage, the content of the corn oligopeptide in the liquid beverage is 1-2g/100ml, and the weight percentage of the corn oligopeptide in the solid beverage is 30-70%.

10. The use of a corn oligopeptide according to claim 1, wherein the corn oligopeptide or the combination of the exercise training and the corn oligopeptide is used for the intervention of exercise-induced myocardial injury.