CN111440715B - Injection device for hair follicle stem cell culture and use method - Google Patents
Injection device for hair follicle stem cell culture and use method Download PDFInfo
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- CN111440715B CN111440715B CN202010313397.5A CN202010313397A CN111440715B CN 111440715 B CN111440715 B CN 111440715B CN 202010313397 A CN202010313397 A CN 202010313397A CN 111440715 B CN111440715 B CN 111440715B
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
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Abstract
The invention discloses an injection device for hair follicle stem cell culture and a use method thereof, wherein the injection device for hair follicle stem cell culture used in the method comprises a storage structure, a first driving structure, a shell, a fixing structure, a storage structure, a second driving structure, a falling structure and a pressurizing structure; the first driving structure is convenient to drive by abutting the elbow, so that the first driving structure drives the two storage structures to be automatically opened and closed; the storage structure is provided with a second driving structure for sealing the storage structure, the inside of the storage structure is provided with a pressurizing structure for pressurizing the digestive agent, the second driving structure is connected with the pressurizing structure, the storage structure is further sealed through the second driving structure when the storage structure is not used, when the liquid medicine is needed to be used, the pressurizing structure is driven to pressurize the liquid medicine in the storage structure to be discharged through the second driving structure, the liquid medicine is more convenient and flexible to use, and meanwhile, the pollution of the liquid medicine is avoided.
Description
Technical Field
The invention relates to a culture device, in particular to an injection device for culturing hair follicle stem cells and a use method thereof, and belongs to the technical field of hair follicle stem cell induction differentiation.
Background
Hair follicle stem cells are one of the hair follicle outer root sheath protrusions of human, are adult stem cells, are in a static state in vivo, show a remarkable proliferation capacity under the in vitro culture effect, and are researched to have multi-directional differentiation potential, can differentiate into epidermis, hair follicle and sebaceous glands and participate in the skin wound healing process, the isolated hair follicle is required to be digested by utilizing separating enzyme in the culture process, the obtained hair follicle Long Tubu is required to be digested by utilizing pancreatin, the conversion direction of the cells required to be cultured is different, the cells are required to be catalyzed and cultured by using different substances, and the digestive medicament in the storage bottle is required to be sucked by using an injection device in the digestion process and then evenly sprayed in a culture dish.
However, when the digestive medicine in the storage bottle is sucked by using the injection device, the storage bottle needs to be frequently opened and closed, the operation is complicated, and the medicine in the storage bottle is easy to pollute, when the medicine amount needed to be used is large, the sucking is needed for a plurality of times, when an operator holds the culture dish with one hand, and when the injection device is held with the other hand, the storage box of the suction pipe of the storage bottle and the injection device is inconvenient to open.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides an injection device for hair follicle stem cell culture and a use method thereof.
In order to solve the technical problems, the injection device for hair follicle stem cell culture provided by the invention comprises a storage structure, a first driving structure, a shell, a fixing structure, a storage structure, a second driving structure, a falling structure and a pressurizing structure; the shell is provided with two containing structures for containing straws and medical wastes, the shell is provided with a fixing structure for fixing the storage structure, the storage structure is clamped with the fixing structure, and the fixing structure and the containing structure are both connected with the first driving structure on the shell; the side wall of the storage structure for storing digestive agent is provided with the falling structure, the storage structure is provided with a second driving structure for sealing the storage structure, the inside of the storage structure is provided with a pressurizing structure for pressurizing the digestive agent, and the second driving structure is connected with the pressurizing structure.
Specifically, the storage structure comprises a cover plate, a storage box, a guide shaft and a torsion spring, wherein the top end of the shell is provided with two storage boxes which are symmetrically arranged, the guide shaft is connected with the storage boxes in a penetrating mode and the cover plate in a rotating mode, the guide shaft is connected with the storage boxes in a rotating mode, the guide shaft is fixed to the cover plate, one end of the torsion spring is fixed to the storage boxes, and the other end of the torsion spring is fixed to the guide shaft.
Specifically, first drive structure includes drive block, latex pad, spout, first reset spring, guide block, stay cord and leading wheel, be equipped with on the shell and fall T shape structure the spout, the drive block with sliding connection between the shell, the bottom of drive block with slide between the spout, the cross-section of the bottom of drive block is regular hexagon structure, be equipped with on the drive block and have the arc and hold the cavity the latex pad, all be equipped with sliding connection on the three angles of spout the guide block, the guide block with sliding connection between the shell, the guide block with be equipped with between the shell first reset spring, first reset spring locates the guide block deviates from the one end of drive block, the one end of stay cord run through in the shell and extend to the guide block, be equipped with on the shell and be used for right the stay cord carries out the guide wheel of direction, wherein two symmetrical the tip of stay cord run through in the containing box and twine in the guiding axle.
Specifically, fixed knot constructs including mount, spacing spring and accomodates the hole, the mount with sliding connection between the shell, another one the tip of stay cord is fixed in the mount, the mount deviates from the one end of stay cord with be equipped with between the shell spacing spring, the equidistance is equipped with a plurality of being used for fixing on the mount accomodate the hole.
Specifically, storage structure includes end cover, one-way admission valve, storage section of thick bamboo, sliding sleeve, slide bar, second reset spring, piston and rubber tube for the cylinder structure of storage digestion liquid medicine storage section of thick bamboo with the mount block, the bottom block of storage section of thick bamboo has the rubber tube, the end cover with threaded connection between the storage section of thick bamboo, be equipped with one-way admission valve on the end cover, the bottom of end cover is equipped with and extends to the inside of storage section of thick bamboo the sliding sleeve, the slide bar with sliding connection between the sliding sleeve, the slide bar with be equipped with between the sliding sleeve second reset spring, the slide bar deviates from the one end of sliding sleeve is equipped with sliding connection the piston, the piston with sliding connection between the storage section of thick bamboo.
Specifically, the structure that drops includes dog, fifth reset spring, first pressure boost room and guiding gutter, the dog with sliding connection between the storage section of thick bamboo, the dog with be equipped with between the storage section of thick bamboo fifth reset spring, the dog with be equipped with between the storage section of thick bamboo first pressure boost room, storage section of thick bamboo with be equipped with annular between the rubber tube switch on in the guiding gutter of first pressure boost room.
Specifically, the second driving structure comprises a pressing block, an air bag, a transmission plug, a storage chamber, a third reset spring, a fourth reset spring and a sealing plug, wherein the pressing block is rotationally connected with the storage cylinder, the pressing block is arranged between the pressing block and the storage cylinder, the transmission plug is in sliding connection with the bottom end of the storage cylinder, the transmission plug and the storage cylinder enclose two storage chambers, the air bag is communicated with one storage chamber at the bottom end, the third reset spring is arranged between the transmission plug and the storage cylinder, the sealing plug perpendicular to the sliding direction of the transmission plug is arranged on the storage cylinder, a through hole communicated with the rubber tube is formed in the sealing plug, and the fourth reset spring is arranged between the sealing plug and the storage cylinder.
Specifically, the boost structure includes connecting rod, pivot, guide bar, connecting block, pressure boost stopper and second pressure boost room, the connecting rod is fixed in the transmission stopper, the connecting rod with sliding connection between the storage section of thick bamboo, the guide bar passes through the pivot with rotate between the storage section of thick bamboo and be connected, the one end of guide bar with roll connection between the connecting rod, the other end of guide bar with roll connection between the connecting block, the tip of connecting block is equipped with the pressure boost stopper that has the through-hole, the pressure boost stopper with enclose into between the sealing plug the second pressure boost room.
Specifically, the application method of the injection device for hair follicle stem cell culture comprises the following steps:
s1: firstly, classifying and storing digestive agents required by different types of cell culture in a plurality of storage structures, and then clamping the storage structures with the fixed structures according to specifications and habits of the storage structures;
s2: when the straws on the storage structure need to be replaced or discarded, driving the two storage structures to be opened through the first driving structure, driving the falling-off structure to enable the straws to fall into one storage structure, and then taking out a new disposable straw from the other storage structure;
s3: when different storage structures are required to be used, the first driving structure drives the fixed structure and the shell to slide, so that the required storage structures slide to a designated position;
s4: when the storage structure is used, the second driving structure is used for controlling the opening and closing of the storage structure, and meanwhile, the pressurizing structure is driven by the second driving structure to pressurize and discharge the liquid medicine into the culture dish.
The beneficial effects of the invention are as follows:
(1) According to the injection device for hair follicle stem cell culture and the use method thereof, the shell is provided with the two containing structures for containing the suction pipe and the medical waste, the containing structures are connected with the first driving structure on the shell, so that the first driving structure is conveniently and contradicted by the elbow to drive the first driving structure, the first driving structure drives the two containing structures to be automatically opened and closed, other operations can be conveniently carried out on the hands, the containing structures are convenient for containing the rubber pipe and the medical waste, and the hair follicle stem cell culture efficiency and the hair follicle stem cell culture quality are greatly improved, namely: the utility model provides a medical waste storage device, including the latex pad, the rubber tube is fixed on the latex pad, make elbow and latex pad conflict, the top of latex pad is equipped with curved accommodation cavity, accord with human structure, the use comfort has been improved, fatigue has been alleviated, locate the inside of two containing boxes respectively with a plurality of rubber tubes and medical waste case according to operating personnel's habit, when needs change the rubber tube and handle medical waste, slide the drive block along the connecting wire direction that is on a parallel with the central line between two containing boxes through elbow, the bottom of drive block is regular hexagon structure, and then make the drive block slide more smoothly in the inside of spout, the drive block is contradicted in the guide block that the containing box corresponds, the first reset spring compression of guide block drive, the distance between guide block and the containing box reduces, and then make torsion spring reset drive guide shaft drive apron automatic opening, the stay cord twines in the guide shaft simultaneously, when loosening the drive block, first reset spring extension drive guide block slides with the spout, make the guide block drive stay cord slide, the stay cord drive apron is airtight with the containing box, torsion spring deformation, and then be convenient for open and close between elbow control apron and the containing box, further be convenient for also take the inside of two and handle the medical waste storage device is convenient for take the high-up the quality of other cells of the medical waste, and handle the quality of the large-size of the rubber tube is greatly improved.
(2) According to the injection device for hair follicle stem cell culture and the use method thereof, the shell is provided with the fixing structure for fixing the storage structure, the storage structure is clamped with the fixing structure, and the fixing structure is connected with the first driving structure on the shell, so that the fixing structure is conveniently driven to slide on the shell through the first driving structure, the distance between the storage structure on the fixing structure and an operator is conveniently adjusted, and the storage structure is more convenient and quicker to take, namely: when the storage structure needs to be accessed, the driving block is pushed and abutted against the guide block corresponding to the fixing frame, so that the guide block drives the first reset spring to compress, and then the limiting spring contracts to drive the fixing frame to slide on the shell, the storage structure which is convenient to need slides to the convenient taking position of the hand of an operator under the driving of the fixing frame, and further the storage of the storage structure is more convenient and quick.
(3) According to the injection device for hair follicle stem cell culture and the use method thereof, the storage structures are clamped with the fixed structure, the consumed liquid medicine is conveniently and directly stored in the storage structures, the phenomenon that the storage bottles are opened reciprocally to enable the digested liquid medicine to be in contact with air and even cause pollution is effectively avoided, namely: the end cover is rotated at first, dismantle the end cover, store the digestion liquid medicine that needs towards the inside of a storage section of thick bamboo one by one, then make and slide between piston and the storage section of thick bamboo, gas between piston and the storage section of thick bamboo is discharged through the one-way exhaust valve on the piston, then twist the end cover, threaded connection between end cover and the storage section of thick bamboo, piston and liquid conflict, the slide bar is accomodate in the inside of sliding sleeve, the second reset spring compression, after the liquid medicine of the inside of a storage section of thick bamboo reduces, gas gets into the inside of end cover through one-way admission valve, the atmospheric pressure at the both ends of messenger's piston is invariable, the second reset spring extends and slides between drive slide bar and the sliding sleeve, the piston of messenger's tip of slide bar is contradicted with the liquid medicine all the time, and then effectually prevented that liquid medicine and air contact from causing the pollution, the injection that takes of messenger's liquid medicine is more convenient and fast.
(4) According to the injection device for hair follicle stem cell culture and the use method, when a new rubber tube is needed to be replaced, the check block is pressed, the check block slides between the check block and the storage cylinder, the check block drives the fifth return spring to compress, the space of the first pressurizing chamber is reduced, gas enters the diversion trench, the pressure of the diversion trench is increased, the gas pressure is higher than the atmospheric pressure, the rubber tube is further conveniently driven to be directly separated from the end part of the storage cylinder, then the new rubber tube is clamped with the end part of the storage cylinder, the check block is released, the fifth return spring drives one end of the check block, which is far away from the fifth return spring, to slide, the inner space of the first pressurizing chamber is enlarged, and air enters the inner part of the first pressurizing chamber through the diversion trench, so that the atmospheric pressure fixes the rubber tube, and the replacement of the new rubber tube is more convenient and quicker.
(5) According to the injection device for culturing hair follicle stem cells and the use method thereof, the storage structure is provided with the second driving structure for sealing the storage structure, the inside of the storage structure is provided with the pressurizing structure for pressurizing the digestive agent, and the second driving structure is connected with the pressurizing structure, so that the storage structure is conveniently sealed through the second driving structure when not in use, and when the medical liquid is needed to be used, the pressurizing structure is driven by the second driving structure to pressurize and drive the medical liquid in the storage structure to be discharged, so that the medical liquid is more convenient and flexible to use, and meanwhile, the pollution of the medical liquid is avoided, namely: when the liquid medicine needs to be used, press the briquetting, rotate between briquetting and the storage section of thick bamboo, the briquetting is contradicted the gasbag, the gasbag deformation, the inside gas of gasbag gets into the inside of the storage chamber that the third reset spring is located, the pneumatic increase drives and slide between transmission stopper and the storage section of thick bamboo, and then make the inside space of another storage chamber reduce, the pressure increase drives sealing plug and storage section of thick bamboo and slide, make fourth reset spring extension, the through-hole on the sealing plug switches on with the second pumping chamber, the transmission stopper drives the connecting rod and slides, the connecting rod drives the guiding rod and revolutes the pivot and rotate, the other end of guiding rod is contradicted the connecting block, the connecting block drives the pressure boost stopper and carries out the pressure boost to the second pumping chamber, the liquid medicine of the inside of messenger's second pumping chamber is discharged from the rubber tube, when not using the liquid medicine, and then make the volume of the storage chamber that the third reset spring is located reduce, the third reset spring drives the transmission stopper and resets, and then make the volume of another storage chamber increase, under the effect of the reset force of atmospheric pressure and its fourth reset spring, make the sealing plug slide to seal the second pumping chamber, the connecting rod drive the connecting rod, the guiding rod drives the guiding rod and resets, the guiding rod passes through the guiding rod and drives the inside of the stopper to rotate around the guiding rod, the inside liquid medicine is more flexible to be discharged to the inside the liquid medicine through the through-hole, and more convenient to avoid the liquid medicine to be polluted.
Drawings
The invention will be further described with reference to the drawings and examples.
FIG. 1 is a schematic diagram of an injection device for hair follicle stem cell culture and a method of use according to a preferred embodiment of the present invention;
FIG. 2 is a schematic diagram illustrating a connection structure between the first driving structure and the housing shown in FIG. 1;
FIG. 3 is an enlarged schematic view of portion A shown in FIG. 1;
FIG. 4 is a schematic structural view of the accommodating structure shown in FIG. 3;
FIG. 5 is a schematic diagram illustrating a connection structure between the storage structure and the second driving structure shown in FIG. 1;
FIG. 6 is an enlarged schematic view of portion B shown in FIG. 5;
FIG. 7 is an enlarged schematic view of portion C shown in FIG. 5;
fig. 8 is a flowchart of a method for using the injection device for hair follicle stem cell culture provided by the invention.
In the figure: 1. the storage structure, 11, the cover plate, 12, the storage box, 13, the guide shaft, 14, the torsion spring, 2, the first driving structure, 21, the driving block, 22, the latex pad, 23, the chute, 24, the first return spring, 25, the guide block, 26, the pull rope, 27, the guide wheel, 3, the housing, 4, the structure, 41, the fixing frame, 42, the limit spring, 43, the storage hole, 5, the storage structure, 51, the end cover, 52, the unidirectional air inlet valve, 53, the storage cylinder, 54, the sliding sleeve, 55, the sliding rod, 56, the second return spring, 57, the piston, 58, the rubber tube, 6, the second driving structure, 61, the pressing block, 62, the air bag, 63, the driving plug, 64, the storage chamber, 65, the third return spring, 66, the fourth return spring, 67, the sealing plug, 7, the falling structure, 71, the stopper, 72, the fifth return spring, 73, the first pressurizing structure, 74, the pressurizing structure, 81, the connecting rod, 82, the rotating shaft, 83, the guide rod, 84, 85, the plug, the pressurizing structure, the 86, and the second pressurizing chamber.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
As shown in fig. 1 to 7, the injection device for hair follicle stem cell culture provided by the invention comprises a storage structure 1, a first driving structure 2, a shell 3, a fixing structure 4, a storage structure 5, a second driving structure 6, a shedding structure 7 and a pressurizing structure 8; the shell 3 is provided with two containing structures 1 for containing straws and medical wastes, the shell 3 is provided with a fixing structure 4 for fixing the storage structure 5, the storage structure 5 is clamped with the fixing structure 4, and the fixing structure 4 and the containing structures 1 are connected with the first driving structure 2 on the shell 3; the side wall of the storage structure 5 for storing digestive agent is provided with the falling structure 7, the storage structure 5 is provided with the second driving structure 6 for sealing the storage structure 5, the inside of the storage structure 5 is provided with the pressurizing structure 8 for pressurizing digestive agent, and the second driving structure 6 is connected with the pressurizing structure 8.
Specifically, the storage structure 1 includes a cover plate 11, a storage box 12, a guide shaft 13 and a torsion spring 14, the top end of the housing 3 is provided with two storage boxes 12 symmetrically arranged, the guide shaft 13 is connected with the storage boxes 12 and the cover plate 11 in a penetrating manner, the guide shaft 13 is connected with the storage boxes 12 in a rotating manner, the guide shaft 13 is fixed on the cover plate 11, one end of the torsion spring 14 is fixed on the storage boxes 12, and the other end of the torsion spring 14 is fixed on the guide shaft 13.
Specifically, the first driving structure 2 includes a driving block 21, a latex pad 22, a chute 23, a first return spring 24, a guide block 25, a pull rope 26 and a guide wheel 27, the chute 23 with an inverted T-shaped structure is provided on the housing 3, the driving block 21 is slidably connected with the housing 3, the bottom end of the driving block 21 slides between the chute 23, the cross section of the bottom end of the driving block 21 is in a regular hexagonal structure, the driving block 21 is provided with the latex pad 22 with an arc-shaped accommodating cavity, three corners of the chute 23 are respectively provided with the guide block 25 in sliding connection, the guide block 25 is slidably connected with the housing 3, the first return spring 24 is provided between the guide block 25 and the housing 3, the first return spring 24 is provided at one end of the guide block 25 facing away from the driving block 21, one end of the pull rope 26 extends through the housing 3 to the guide block 25, the housing 3 is provided with the guide block 22 for guiding the pull rope 26, and the two symmetrical end portions 27 of the pull rope 26 are wound around the housing 12; be equipped with two on the shell 3 be used for accomodating straw and medical waste accomodate structure 1, accomodate structure 1 connect in on the shell 3 first drive structure 2, and then be convenient for through the elbow conflict drive first drive structure 2 makes the drive of first drive structure 2 two accomodate structure 1 is automatic to be opened and close, makes the hand also can be more convenient for carry out other operations, accomodate structure 1's setting is convenient for accomodate rubber tube 58 and medical waste, and then improved hair follicle stem cell culture's efficiency and quality greatly, namely: firstly, the elbow is abutted against the latex pad 22, the top end of the latex pad 22 is provided with an arc-shaped containing cavity, which accords with the human body structure, improves the use comfort, relieves fatigue, a plurality of rubber tubes 58 and medical waste bins are respectively arranged in the two containing boxes 12 according to the habit of operators, when the rubber tubes 58 need to be replaced and medical waste is treated, the driving block 21 slides along the direction of a connecting line parallel to the central line between the two containing boxes 12 through the elbow, the bottom end of the driving block 21 is of a regular hexagon structure, so that the driving block 21 slides in the sliding groove 23 more smoothly, and when the driving block 21 abuts against the guiding block 25 corresponding to the containing box 12, the guiding block 25 drives the first reset spring 24 to compress, the distance between the guide block 25 and the storage box 12 is reduced, the torsion spring 14 is further reduced to drive the guide shaft 13 to drive the cover plate 11 to automatically open, meanwhile, the pull rope 26 is wound on the guide shaft 13, when the driving block 21 is loosened, the first return spring 24 stretches to drive the guide block 25 and the sliding chute 23 to slide, the guide block 25 drives the pull rope 26 to slide, the pull rope 26 drives the guide shaft 13 to rotate, the guide shaft 13 drives the cover plate 11 to abut against the storage box 12 to be airtight, the torsion spring 14 deforms, so that the opening and closing between the cover plate 11 and the storage box 12 are controlled through an elbow, other operations can be more conveniently performed by hands, the rubber tube 58 and medical waste are conveniently taken and treated respectively in the two storage boxes 12, thereby greatly improving the efficiency and quality of hair follicle stem cell culture.
Specifically, the fixing structure 4 includes a fixing frame 41, a limiting spring 42, and a receiving hole 43, where the fixing frame 41 is slidably connected with the housing 3, an end of the other pull cord 26 is fixed to the fixing frame 41, the limiting spring 42 is disposed between one end of the fixing frame 41 facing away from the pull cord 26 and the housing 3, and a plurality of receiving holes 43 for fixing the storage structure 5 are equidistantly disposed on the fixing frame 41; be equipped with on the shell 3 and be used for fixing the fixed knot of storage structure 5 constructs 4, storage structure 5 with the block between the fixed knot constructs 4, just fixed knot constructs 4 connect in on the shell 3 first drive structure 2, and then be convenient for pass through first drive structure 2 drive fixed knot constructs 4 is in slide on the shell 3, be convenient for adjust the distance of storage structure 5 on the fixed knot constructs 4 apart from operating personnel, and then makes the taking storage structure 5 convenient and fast more, namely: when the storage structure 5 needs to be accessed, the driving block 21 is pushed, the driving block 21 is abutted against the guide block 25 corresponding to the fixing frame 41, so that the guide block 25 drives the first reset spring 24 to compress, and then the limit spring 42 contracts and drives the fixing frame 41 to slide on the shell 2, the storage structure 5 which is convenient to need slides to the position where an operator is convenient to take under the driving of the fixing frame 41, and further the storage of the storage structure 5 is more convenient and quicker.
Specifically, the storage structure 5 includes an end cover 51, a unidirectional air inlet valve 52, a storage cylinder 53, a sliding sleeve 54, a sliding rod 55, a second return spring 56, a piston 57 and a rubber tube 58, the storage cylinder 53 of a cylindrical structure for storing digestive liquid medicine is clamped with the fixing frame 41, the bottom end of the storage cylinder 53 is clamped with the rubber tube 58, the end cover 51 is in threaded connection with the storage cylinder 53, the end cover 51 is provided with the unidirectional air inlet valve 52, the bottom end of the end cover 51 is provided with the sliding sleeve 54 extending into the storage cylinder 53, the sliding rod 55 is in sliding connection with the sliding sleeve 54, the second return spring 56 is arranged between the sliding rod 55 and the sliding sleeve 54, one end of the sliding rod 55, which is far away from the sliding sleeve 54, is provided with the piston 57 in sliding connection, and the piston 57 is in sliding connection with the storage cylinder 53; a plurality of storage structure 5 with fixed knot constructs 4 block, storage structure 5's setting is convenient for will consume the direct storage of liquid medicine in storage structure 5's inside, effectually avoided reciprocal opening storage bottle, make digestion liquid medicine and air contact cause the pollution even, namely: the end cover 51 is rotated at first, the end cover 51 is disassembled, needed digestion liquid medicine is stored in the storage cylinder 53 one by one, then the piston 57 and the storage cylinder 53 slide, air between the piston 57 and the storage cylinder 53 is discharged through a one-way exhaust valve on the piston 57, then the end cover 51 is screwed, the end cover 51 is in threaded connection with the storage cylinder 53, the piston 57 is in interference with liquid, the sliding rod 55 is accommodated in the sliding sleeve 54, the second return spring 56 is compressed, after the liquid medicine in the storage cylinder 53 is reduced, the air enters the end cover 51 through the one-way air inlet valve 52, so that the air pressure at two ends of the piston 57 is constant, the second return spring 56 stretches and drives the sliding rod 55 to slide between the sliding sleeve 54, the piston 57 at the end part of the sliding rod 55 is always in interference with the liquid medicine, pollution caused by contact with the air is effectively prevented, and the liquid medicine is taken out and injected more conveniently and rapidly.
Specifically, the falling structure 7 includes a stop block 71, a fifth return spring 72, a first plenum 73 and a flow guiding groove 74, the stop block 71 is slidably connected with the storage barrel 53, the fifth return spring 72 is disposed between the stop block 71 and the storage barrel 53, the first plenum 73 is disposed between the stop block 71 and the storage barrel 53, and the flow guiding groove 74, which is annularly communicated with the first plenum 73, is disposed between the storage barrel 53 and the rubber tube 58; the side wall of the storage structure 5 for storing digestive reagents is provided with the falling structure 7, so that the replacement of the new rubber tube 58 is more convenient and quick, namely: when a new rubber tube 58 needs to be replaced, the stop block 71 is pressed, the stop block 71 slides with the storage tube 53, the stop block 71 drives the fifth return spring 72 to compress, the space of the first pressurizing chamber 73 is reduced, gas enters the diversion trench 74, the pressure of the diversion trench 74 is increased, the gas pressure is enabled to be higher than the atmospheric pressure, the rubber tube 58 is further conveniently driven to be directly separated from the end of the storage tube 53, then the new rubber tube 58 is clamped with the end of the storage tube 53, the stop block 71 is released, the fifth return spring 72 drives one end of the stop block 71, which is far away from the fifth return spring 72, to slide, the inner space of the first pressurizing chamber 73 is enlarged, the air enters the inner space of the first pressurizing chamber 73 through the diversion trench 74, the rubber tube 58 is further fixed by the atmospheric pressure, and the new rubber tube 58 is further replaced more conveniently and rapidly.
Specifically, the second driving structure 6 includes a pressing block 61, an air bag 62, a transmission plug 63, a storage chamber 64, a third return spring 65, a fourth return spring 66 and a sealing plug 67, the pressing block 61 is rotationally connected with the storage barrel 53, the air bag 62 is disposed between the pressing block 61 and the storage barrel 53, the transmission plug 63 in sliding connection is disposed at the bottom end of the storage barrel 53, the transmission plug 63 and the storage barrel 53 enclose two storage chambers 64, the air bag 62 is communicated with one storage chamber 64 at the bottom end, the third return spring 65 is disposed between the transmission plug 63 and the storage barrel 53, the sealing plug 67 perpendicular to the sliding direction of the transmission plug 63 is disposed on the storage barrel 53, a through hole communicated with the rubber tube 58 is disposed on the sealing plug 67, and the fourth return spring 66 is disposed between the sealing plug 67 and the storage barrel 53.
Specifically, the pressurizing structure 8 includes a connecting rod 81, a rotating shaft 82, a guide rod 83, a connecting block 84, a pressurizing plug 85 and a second pressurizing chamber 86, the connecting rod 81 is fixed on the transmission plug 63, the connecting rod 81 is slidingly connected with the storage barrel 53, the guide rod 83 is rotationally connected with the storage barrel 53 through the rotating shaft 82, one end of the guide rod 83 is in rolling connection with the connecting rod 81, the other end of the guide rod 83 is in rolling connection with the connecting block 84, the end part of the connecting block 84 is provided with the pressurizing plug 85 with a through hole, and the second pressurizing chamber 86 is enclosed between the pressurizing plug 85 and the sealing plug 67; be equipped with on the storage structure 5 be used for right the storage structure 5 carry out sealed second drive structure 6, the inside of storage structure 5 is equipped with and is used for carrying out the pressure boost structure 8 of supercharged to the digestion medicament, just second drive structure 6 connect in pressure boost structure 8, and then be convenient for when not using pass through second drive structure 6 seal the storage structure 5, when needs use the liquid medicine, through second drive structure 6 drive pressure boost structure 8 supercharged drive the inside liquid medicine discharge of storage structure 5, and then make the use of liquid medicine more convenient nimble, avoided the pollution of liquid medicine simultaneously, namely: when the medical solution is needed to be used, the pressing block 61 is pressed, the pressing block 61 rotates with the storage cylinder 53, the pressing block 61 props against the air bag 62, the air bag 62 deforms, air in the air bag 62 enters the storage chamber 64 where the third return spring 65 is located, the air pressure is increased to drive the transmission plug 63 to slide with the storage cylinder 53, the space in the other storage chamber 64 is further reduced, the pressure is increased to drive the sealing plug 67 to slide with the storage cylinder 53, the fourth return spring 66 is extended, the through hole on the sealing plug 67 is communicated with the second pressurizing chamber 86, the transmission plug 63 drives the connecting rod 81 to slide, the connecting rod 81 drives the guide rod 83 to rotate around the rotating shaft 82, the other end of the guide rod 83 props against the connecting block 84, the connecting block 84 drives the pressurizing plug 85 to pressurize the second pressurizing chamber 86, so that the liquid medicine in the second pressurizing chamber 86 is discharged from the rubber tube 58, when the liquid medicine is not used, the air bag 62 is reset, the volume of the storage chamber 64 where the third restoring spring 65 is positioned is further reduced, the third restoring spring 65 drives the transmission plug 63 to reset, the volume of the other storage chamber 64 is further increased, under the action of the atmospheric pressure and the restoring force of the fourth restoring spring 66, the sealing plug 67 slides to seal the second pressurizing chamber 86, the transmission plug 63 drives the connecting rod 81 to reset, the connecting rod 81 drives the guide rod 83 to reset, the guide rod 83 drives the pressurizing plug 85 to reset through the connecting block 84, the liquid medicine in the storage barrel 53 is discharged into the second pressurizing chamber 86 through the through hole in the pressurizing plug 85, so that the use of the liquid medicine is more convenient and flexible, and the pollution of the liquid medicine is avoided.
Specifically, referring to fig. 8 in combination, fig. 8 is a flowchart of a method for using the injection device for hair follicle stem cell culture according to the present invention, and the method for using the injection device for hair follicle stem cell culture comprises the following steps:
s1: firstly, classifying and storing digestive agents required by different types of cell culture in a plurality of storage structures 5, and then clamping the storage structures 5 with the fixed structures 4 according to specifications and habits thereof; the end cover 51 is rotated, the end cover 51 is disassembled, needed digestion liquid medicine is stored in the storage cylinder 53 one by one, then the piston 57 and the storage cylinder 53 slide, gas between the piston 57 and the storage cylinder 53 is discharged through a one-way exhaust valve on the piston 57, then the end cover 51 is screwed, the end cover 51 and the storage cylinder 53 are in threaded connection, the piston 57 is in interference with liquid, the sliding rod 55 is accommodated in the sliding sleeve 54, the second return spring 56 is compressed, after the liquid medicine in the storage cylinder 53 is reduced, the gas enters the end cover 51 through the one-way air inlet valve 52, so that the air pressure at two ends of the piston 57 is constant, the second return spring 56 stretches and drives the sliding rod 55 and the sliding sleeve 54 to slide, the piston 57 at the end part of the sliding rod 55 is always in interference with the liquid medicine, pollution caused by the contact of the liquid medicine and the air is effectively prevented, and the liquid medicine is taken out and injected more conveniently and rapidly;
S2: when the straws on the storage structure 5 need to be replaced or discarded, the first driving structure 2 drives the two storage structures 1 to be opened, then drives the falling-off structure 7 to enable the straws to fall off to the inside of one storage structure 1, and then takes out a new disposable straw from the other storage structure 1; firstly, the elbow is abutted against the latex pad 22, the top end of the latex pad 22 is provided with an arc-shaped containing cavity, which accords with the human body structure, thereby improving the use comfort, relieving fatigue, a plurality of rubber tubes 58 and medical waste bins are respectively arranged in the two containing boxes 12 according to the habit of operators, when the rubber tubes 58 need to be replaced and medical waste is treated, the driving block 21 slides along the direction of a connecting line parallel to the central line between the two containing boxes 12 through the elbow, the bottom end of the driving block 21 is of a regular hexagon structure, so that the driving block 21 slides in the inside of the chute 23 more smoothly, when the driving block 21 abuts against the guiding block 25 corresponding to the containing box 12, the guiding block 25 drives the first reset spring 24 to compress, the distance between the guiding block 25 and the containing box 12 is reduced, and then the torsion spring 14 is reset to drive the guide shaft 13 to drive the cover plate 11 to automatically open, when a new rubber tube 58 needs to be replaced, the stop block 71 is pressed, the stop block 71 slides between the stop block 71 and the storage cylinder 53, the stop block 71 drives the fifth return spring 72 to compress, the space of the first plenum 73 is reduced, the air enters the guide groove 74, the pressure of the guide groove 74 is increased, the air pressure is higher than the atmospheric pressure, so that the end of the rubber tube 58 and the end of the storage cylinder 53 are driven to be directly separated, then the new rubber tube 58 is clamped with the end of the storage cylinder 53 to release the stop block 71, the fifth return spring 72 drives one end of the stop block 71 deviating from the fifth return spring 72 to slide, the inner space of the first plenum 73 is enlarged, air enters the first pressurizing chamber 73 through the diversion trench 74, so that the rubber tube 58 is fixed by the atmospheric pressure, and the rubber tube 58 is more convenient and quick to replace; meanwhile, when the driving block 21 is loosened, the first return spring 24 stretches to drive the guiding block 25 and the sliding chute 23 to slide, so that the guiding block 25 drives the pulling rope 26 to slide, the pulling rope 26 drives the guiding shaft 13 to rotate, the guiding shaft 13 drives the cover plate 11 to abut against the containing box 12 to be airtight, the torsion spring 14 deforms, and further, the opening and closing between the cover plate 11 and the containing box 12 are controlled by the elbow of an arm, so that other operations can be more conveniently carried out on the hands, the rubber tube 58 and medical waste treatment can be conveniently taken in the two containing boxes 12 respectively, and further, the efficiency and quality of hair follicle stem cell culture are greatly improved;
S3: when the different storage structures 5 are required to be used, the first driving structure 2 drives the fixed structure 4 and the shell 3 to slide, so that the required storage structures 5 slide to a designated position; when the storage structure 5 needs to be accessed, the driving block 21 is pushed, the driving block 21 is abutted against the guide block 25 corresponding to the fixing frame 41, so that the guide block 25 drives the first reset spring 24 to compress, and further the limit spring 42 contracts to drive the fixing frame 41 to slide on the shell 2, the storage structure 5 which is convenient to need slides to a position where an operator is convenient to take under the driving of the fixing frame 41, and further the storage of the storage structure 5 is more convenient and faster;
s4: when the storage structure 5 is used, the second driving structure 6 is used for controlling the opening and closing of the storage structure 5, and meanwhile, the pressurizing structure 8 is driven by the second driving structure 6 to pressurize and discharge the liquid medicine into the culture dish; when the medical solution is needed to be used, the pressing block 61 is pressed, the pressing block 61 rotates with the storage cylinder 53, the pressing block 61 props against the air bag 62, the air bag 62 deforms, air in the air bag 62 enters the storage chamber 64 where the third return spring 65 is located, the air pressure is increased to drive the transmission plug 63 to slide with the storage cylinder 53, the space in the other storage chamber 64 is further reduced, the pressure is increased to drive the sealing plug 67 to slide with the storage cylinder 53, the fourth return spring 66 is extended, the through hole on the sealing plug 67 is communicated with the second pressurizing chamber 86, the transmission plug 63 drives the connecting rod 81 to slide, the connecting rod 81 drives the guide rod 83 to rotate around the rotating shaft 82, the other end of the guide rod 83 props against the connecting block 84, the connecting block 84 drives the pressurizing plug 85 to pressurize the second pressurizing chamber 86, so that the liquid medicine in the second pressurizing chamber 86 is discharged from the rubber tube 58, when the liquid medicine is not used, the air bag 62 is reset, the volume of the storage chamber 64 where the third restoring spring 65 is positioned is further reduced, the third restoring spring 65 drives the transmission plug 63 to reset, the volume of the other storage chamber 64 is further increased, under the action of the atmospheric pressure and the restoring force of the fourth restoring spring 66, the sealing plug 67 slides to seal the second pressurizing chamber 86, the transmission plug 63 drives the connecting rod 81 to reset, the connecting rod 81 drives the guide rod 83 to reset, the guide rod 83 drives the pressurizing plug 85 to reset through the connecting block 84, the liquid medicine in the storage barrel 53 is discharged into the second pressurizing chamber 86 through the through hole in the pressurizing plug 85, so that the use of the liquid medicine is more convenient and flexible, and the pollution of the liquid medicine is avoided.
When the invention is used, firstly, the end cover 51 is rotated, the end cover 51 is disassembled, needed digestion liquid medicine is stored in the storage cylinder 53 one by one, then the piston 57 and the storage cylinder 53 slide, gas between the piston 57 and the storage cylinder 53 is discharged through the one-way exhaust valve on the piston 57, then the end cover 51 is screwed, the end cover 51 is connected with the storage cylinder 53 in a threaded manner, the piston 57 is in interference with liquid, the slide bar 55 is accommodated in the sliding sleeve 54, the second return spring 56 compresses, after the liquid medicine in the storage cylinder 53 is reduced, the gas enters the interior of the end cover 51 through the one-way air inlet valve 52, so that the air pressure at two ends of the piston 57 is constant, the second return spring 56 stretches and drives the slide bar 55 to slide with the sliding sleeve 54, the piston 57 at the end part of the slide bar 55 is always in interference with the liquid medicine, pollution caused by the contact of the liquid medicine with air is effectively prevented, the taking and injecting of the liquid medicine is more convenient, the elbow is made to collide with the latex pad 22, the top end of the latex pad 22 is provided with an arc-shaped containing cavity, which accords with the human body structure, the use comfort is improved, fatigue is relieved, a plurality of rubber tubes 58 and medical waste bins are respectively arranged in the two containing bins 12 according to the habit of operators, when the rubber tubes 58 are required to be replaced and medical waste is treated, the driving block 21 is slid along the direction of a connecting line parallel to the central line between the two containing bins 12 by the elbow, the bottom end of the driving block 21 is of a regular hexagon structure, the driving block 21 slides in the sliding groove 23 more smoothly, when the driving block 21 collides with the corresponding guide block 25 of the containing bins 12, the guide block 25 drives the first reset spring 24 to compress, the distance between the guide block 25 and the containing bins 12 is reduced, and the torsion spring 14 is reset to drive the guide shaft 13 to drive the cover plate 11 to be automatically opened, when a new rubber tube 58 needs to be replaced, the stop block 71 is pressed, the stop block 71 slides with the storage barrel 53, the stop block 71 drives the fifth return spring 72 to compress, the space of the first pressurizing chamber 73 is reduced, gas enters the guide groove 74, the pressure of the guide groove 74 is increased, the gas pressure is higher than the atmospheric pressure, the rubber tube 58 is further conveniently driven to be directly separated from the end part of the storage barrel 53, then the new rubber tube 58 is clamped with the end part of the storage barrel 53, the stop block 71 is released, the fifth return spring 72 drives one end of the stop block 71, which is far away from the fifth return spring 72, to slide, the inner space of the first pressurizing chamber 73 is enlarged, the air enters the inner part of the first pressurizing chamber 73 through the guide groove 74, and the rubber tube 58 is further fixed by the atmospheric pressure, so that the new rubber tube 58 is more convenient and rapid to replace; meanwhile, the pull rope 26 is wound on the guide shaft 13, when the driving block 21 is loosened, the first reset spring 24 stretches to drive the guide block 25 and the sliding groove 23 to slide, so that the guide block 25 drives the pull rope 26 to slide, the pull rope 26 drives the guide shaft 13 to rotate, the guide shaft 13 drives the cover plate 11 to abut against the containing boxes 12 to be airtight, the torsion spring 14 deforms, further, the opening and closing between the cover plate 11 and the containing boxes 12 are controlled through the elbow, the hands can be more convenient for other operations, the rubber tube 58 and the medical waste are conveniently taken out of the two containing boxes 12 respectively, the efficiency and the quality of hair follicle stem cell culture are greatly improved, when the storage structure 5 is required to be accessed, the driving block 21 is pushed, the driving block 21 abuts against the guide block 25 corresponding to the fixing frame 41, the guide block 25 drives the first reset spring 24 to compress, and the limit spring 42 contracts to drive the fixing frame 41 to slide on the shell 2, the storage structure 5 which is convenient to be needed slides to the hand of the operator to be convenient to take the position under the drive of the fixing frame 41, so that the storage of the storage structure 5 is more convenient and quicker, when the liquid medicine is needed to be used, the pressing block 61 is pressed, the pressing block 61 rotates with the storage cylinder 53, the pressing block 61 props against the air bag 62, the air bag 62 deforms, the air in the air bag 62 enters the storage chamber 64 where the third return spring 65 is positioned, the air pressure is increased to drive the transmission plug 63 to slide with the storage cylinder 53, the space in the other storage chamber 64 is further reduced, the pressure is increased to drive the sealing plug 67 to slide with the storage cylinder 53, the fourth return spring 66 is extended, the through hole on the sealing plug 67 is communicated with the second pressurizing chamber 86, the transmission plug 63 drives the connecting rod 81 to slide, the connecting rod 81 drives the guide rod 83 to rotate around the rotating shaft 82, the other end of the guide rod 83 props against the connecting block 84, the connecting block 84 drives the pressurizing plug 85 to pressurize the second pressurizing chamber 86, so that the liquid medicine in the second pressurizing chamber 86 is discharged from the rubber tube 58, when the liquid medicine is not used, the air bag 62 is reset, the volume of the storage chamber 64 where the third restoring spring 65 is located is further reduced, the third restoring spring 65 drives the transmission plug 63 to be reset, the volume of the other storage chamber 64 is further increased, the sealing plug 67 slides to seal the second pressurizing chamber 86 under the action of the atmospheric pressure and the restoring force of the fourth restoring spring 66, the transmission plug 63 drives the connecting rod 81 to be reset, the connecting rod 81 drives the guide rod 83 to be reset, the guide rod 83 drives the pressurizing plug 85 to be reset through the connecting block 84, the liquid medicine in the storage barrel 53 is discharged into the second pressurizing chamber 86 through the through hole in the pressurizing plug 85, the use of the liquid medicine is more convenient and flexible, and meanwhile, the pollution of the liquid medicine is avoided.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are, therefore, to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Furthermore, it should be understood that although the present disclosure describes embodiments, not every embodiment is provided with a separate embodiment, and that this description is provided for clarity only, and that the disclosure is not limited to the embodiments described in detail below, and that the embodiments described in the examples may be combined as appropriate to form other embodiments that will be apparent to those skilled in the art.
Claims (2)
1. An injection device for hair follicle stem cell culture is characterized by comprising a storage structure (1), a first driving structure (2), a shell (3), a fixing structure (4), a storage structure (5), a second driving structure (6), a falling-off structure (7) and a pressurizing structure (8); the medical waste storage device comprises a shell (3), wherein two storage structures (1) for storing a straw and medical waste are arranged on the shell (3), a fixing structure (4) for fixing a storage structure (5) is arranged on the shell (3), the storage structure (5) is clamped with the fixing structure (4), and the fixing structure (4) and the storage structure (1) are connected to a first driving structure (2) on the shell (3); the side wall of the storage structure (5) for storing digestive agents is provided with the falling-off structure (7), the storage structure (5) is provided with the second driving structure (6) for sealing the storage structure (5), the inside of the storage structure (5) is provided with the pressurizing structure (8) for pressurizing digestive agents, and the second driving structure (6) is connected with the pressurizing structure (8);
The accommodating structure (1) comprises a cover plate (11), an accommodating box (12), a guide shaft (13) and a torsion spring (14), wherein the two accommodating boxes (12) which are symmetrically arranged are arranged at the top end of the shell (3), the guide shaft (13) is connected with the accommodating box (12) and the cover plate (11) in a penetrating manner, the guide shaft (13) is rotationally connected with the accommodating boxes (12), the guide shaft (13) is fixed on the cover plate (11), one end of the torsion spring (14) is fixed on the accommodating box (12), and the other end of the torsion spring (14) is fixed on the guide shaft (13);
the first driving structure (2) comprises a driving block (21), a latex pad (22), a sliding groove (23), a first reset spring (24), a guide block (25), a pull rope (26) and a guide wheel (27), wherein the sliding groove (23) with an inverted T-shaped structure is arranged on the shell (3), the driving block (21) is in sliding connection with the shell (3), the bottom end of the driving block (21) and the sliding groove (23) slide, the cross section of the bottom end of the driving block (21) is of a regular hexagon structure, the driving block (21) is provided with the latex pad (22) with an arc-shaped containing cavity, the guide block (25) with the guide wheel (27) is arranged on three corners of the sliding groove (23), the guide block (25) is in sliding connection with the shell (3), the first reset spring (24) is arranged between the guide block (25) and the shell (3), the first reset spring (24) is arranged on the bottom end of the guide block (25) deviating from the driving block (21), the latex pad (22) is arranged on one end of the guide block (21) and penetrates through the shell (26) to extend to the guide wheel (3), the end parts of the two symmetrical pull ropes (26) penetrate through the storage box (12) and are wound on the guide shaft (13);
The fixing structure (4) comprises a fixing frame (41), a limiting spring (42) and a containing hole (43), wherein the fixing frame (41) is connected with the shell (3) in a sliding mode, the other end portion of the pull rope (26) is fixed to the fixing frame (41), the limiting spring (42) is arranged between one end, deviating from the pull rope (26), of the fixing frame (41) and the shell (3), and a plurality of containing holes (43) used for fixing the storage structure (5) are formed in the fixing frame (41) at equal intervals;
the storage structure (5) comprises an end cover (51), a one-way air inlet valve (52), a storage cylinder (53), a sliding sleeve (54), a sliding rod (55), a second reset spring (56), a piston (57) and a rubber tube (58), wherein the storage cylinder (53) is used for storing digestion liquid medicine, the storage cylinder (53) is clamped with the fixing frame (41), the bottom end of the storage cylinder (53) is clamped with the rubber tube (58), the end cover (51) is in threaded connection with the storage cylinder (53), the end cover (51) is provided with the one-way air inlet valve (52), the bottom end of the end cover (51) is provided with the sliding sleeve (54) extending into the storage cylinder (53), the sliding rod (55) is in sliding connection with the sliding sleeve (54), the second reset spring (56) is arranged between the sliding rod (55) and one end of the sliding rod (55) deviating from the sliding sleeve (54) is provided with the piston (57) in sliding connection, and the piston (57) is in sliding connection with the storage cylinder (53);
The falling structure (7) comprises a stop block (71), a fifth return spring (72), a first pressurizing chamber (73) and a diversion trench (74), wherein the stop block (71) is in sliding connection with the storage barrel (53), the fifth return spring (72) is arranged between the stop block (71) and the storage barrel (53), the first pressurizing chamber (73) is arranged between the stop block (71) and the storage barrel (53), and the diversion trench (74) which is annularly communicated with the first pressurizing chamber (73) is arranged between the storage barrel (53) and the rubber tube (58);
the second driving structure (6) comprises a pressing block (61), an air bag (62), a transmission plug (63), a storage chamber (64), a third reset spring (65), a fourth reset spring (66) and a sealing plug (67), wherein the pressing block (61) is rotationally connected with the storage barrel (53), the air bag (62) is arranged between the pressing block (61) and the storage barrel (53), the transmission plug (63) which is in sliding connection is arranged at the bottom end of the storage barrel (53), the transmission plug (63) and the storage barrel (53) enclose into two storage chambers (64), one storage chamber (64) at the bottom end of the air bag (62) is communicated with the air bag, the third reset spring (65) is arranged between the transmission plug (63) and the storage barrel (53), the sealing plug (67) which is perpendicular to the sliding direction of the transmission plug (63) is arranged on the storage barrel (53), a through hole which is communicated with the rubber pipe (58) is arranged on the sealing plug (67), and the fourth reset spring (66) is arranged between the air bag (62) and the storage barrel (53).
The supercharging structure (8) comprises a connecting rod (81), a rotating shaft (82), a guide rod (83), a connecting block (84), a supercharging plug (85) and a second supercharging chamber (86), wherein the connecting rod (81) is fixed on the transmission plug (63), the connecting rod (81) is connected with the storage cylinder (53) in a sliding mode, the guide rod (83) is connected with the storage cylinder (53) in a rotating mode through the rotating shaft (82), one end of the guide rod (83) is connected with the connecting rod (81) in a rolling mode, the other end of the guide rod (83) is connected with the connecting block (84) in a rolling mode, the end portion of the connecting block (84) is provided with the supercharging plug (85) with a through hole, and the second supercharging chamber (86) is formed by enclosing between the supercharging plug (85) and the sealing plug (67).
2. The method of using an injection device for hair follicle stem cell culture according to claim 1, wherein: the method comprises the following steps:
s1: firstly, classifying and storing digestive agents required by different types of cell culture in a plurality of storage structures (5), and then clamping the storage structures (5) with the fixed structures (4) according to specifications and habits thereof;
s2: when the straws on the storage structure (5) need to be replaced or discarded, driving the two containing structures (1) to be opened through the first driving structure (2), then driving the falling-off structure (7) to enable the straws to fall off to the inside of one containing structure (1), and then taking out a new disposable straw from the other containing structure (1);
S3: when different storage structures (5) are required to be used, the first driving structure (2) drives the fixed structure (4) to slide with the shell (3), so that the required storage structures (5) slide to a designated position;
s4: when the storage structure (5) is used, the second driving structure (6) is used for controlling the opening and closing of the storage structure (5), and meanwhile, the pressurizing structure (8) is driven by the second driving structure (6) to pressurize and discharge the liquid medicine into the culture dish.
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| CN202010313397.5A CN111440715B (en) | 2020-04-20 | 2020-04-20 | Injection device for hair follicle stem cell culture and use method |
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| CN202010313397.5A CN111440715B (en) | 2020-04-20 | 2020-04-20 | Injection device for hair follicle stem cell culture and use method |
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| CN111440715B true CN111440715B (en) | 2023-09-29 |
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| US4447225A (en) * | 1982-03-22 | 1984-05-08 | Taff Barry E | Multidose jet injector |
| CN102586177A (en) * | 2012-02-15 | 2012-07-18 | 北京雍禾植发技术研究院 | Culture method of hair follicle stem cell for treating baldness, as well as syringe |
| CN110305944A (en) * | 2019-06-26 | 2019-10-08 | 王中刚 | A kind of cool-down method for hereditary tumor genetic test |
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| CN106390237B (en) * | 2015-08-03 | 2020-03-17 | 重庆倍加医疗器械有限公司 | Syringe with a needle |
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