CN111440715A - Injection device for hair follicle stem cell culture and use method - Google Patents

Injection device for hair follicle stem cell culture and use method Download PDF

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CN111440715A
CN111440715A CN202010313397.5A CN202010313397A CN111440715A CN 111440715 A CN111440715 A CN 111440715A CN 202010313397 A CN202010313397 A CN 202010313397A CN 111440715 A CN111440715 A CN 111440715A
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storage
block
driving
storage cylinder
pressurizing
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CN111440715B (en
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赵焰
孙吉灵
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Guizhou Saier Biotechnology Co ltd
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Jinan Shengfatang Biotechnology Co ltd
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    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/18Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0625Epidermal cells, skin cells; Cells of the oral mucosa
    • C12N5/0627Hair cells
    • C12N5/0628Hair stem cells; Hair progenitors
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00Methods for the dissociation of cells, e.g. specific use of enzymes

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Abstract

The invention discloses an injection device for culturing hair follicle stem cells and a using method thereof, wherein the injection device for culturing the hair follicle stem cells used in the method comprises a receiving structure, a first driving structure, a shell, a fixing structure, a storage structure, a second driving structure, an off-body structure and a pressurizing structure; the first driving structure is driven by the elbow to abut against the first driving structure, so that the first driving structure drives the two containing structures to be automatically opened and closed; the storage structure is provided with a second driving structure used for sealing the storage structure, the inside of the storage structure is provided with a pressurizing structure used for pressurizing the digestion medicine, the second driving structure is connected to the pressurizing structure, and further the storage structure is convenient to seal through the second driving structure when not in use, when the medicine liquid is needed to be used, the medicine liquid in the inside of the storage structure is driven to be pressurized through the second driving structure to be discharged, and further the use of the medicine liquid is more convenient and flexible, and meanwhile, the pollution of the medicine liquid is avoided.

Description

Injection device for hair follicle stem cell culture and use method
Technical Field
The invention relates to a culture device, in particular to an injection device for hair follicle stem cell culture and a use method thereof, belonging to the technical field of hair follicle stem cell induced differentiation.
Background
The hair follicle stem cells are one of the hair follicle outer root sheath bulge parts of a human, belong to adult stem cells, are in a static state in vivo, show remarkable proliferation capacity under the action of in vitro culture, and are researched and found to have multidirectional differentiation potential, can be differentiated into epidermis, hair follicles and sebaceous glands, participate in the process of healing skin wounds, in the culture process, the isolated hair follicles need to be digested by separating enzyme, the obtained hair follicle bulge parts are digested by pancreatin, different substances need to be used for catalyzing and culturing the cells when the direction of the conversion of the cells needing to be cultured is different, in the digestion treatment process, an injection device needs to be used for sucking a digestive medicament in the storage bottle, and then the digestive medicament is uniformly sprayed in a culture dish.
However, when the injection device is used to suck the digestive medicament in the storage bottle, the storage bottle needs to be opened and closed frequently, the operation is complicated, the medicament in the storage bottle is easily polluted, when the required medicament amount is large, the suction needs to be carried out for many times, when an operator holds the culture dish with one hand and holds the injection device with the other hand, the storage box of the storage bottle and the suction pipe of the injection device is not convenient to open.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides an injection device for hair follicle stem cell culture and a using method thereof.
In order to solve the technical problem, the injection device for culturing the hair follicle stem cells provided by the invention comprises a containing structure, a first driving structure, a shell, a fixing structure, a storage structure, a second driving structure, a shedding structure and a pressurizing structure; the shell is provided with two containing structures for containing a suction pipe and medical waste, the shell is provided with a fixing structure for fixing the storage structure, the storage structure and the fixing structure are clamped, and the fixing structure and the containing structure are both connected to the first driving structure on the shell; the side wall of the storage structure is provided with the falling structure, the storage structure is provided with a second driving structure, the second driving structure is used for sealing the storage structure, the inside of the storage structure is provided with a pressurizing structure used for pressurizing the digestion medicine, and the second driving structure is connected with the pressurizing structure.
Specifically, the storage structure comprises a cover plate, a storage box, a guide shaft and a torsion spring, the two storage boxes are symmetrically arranged at the top end of the shell, the guide shaft is connected with the storage box and the cover plate in a penetrating manner, the guide shaft is rotatably connected with the storage box, the guide shaft is fixed on the cover plate, one end of the torsion spring is fixed on the storage box, and the other end of the torsion spring is fixed on the guide shaft.
Specifically, the first driving structure comprises a driving block, a latex pad, a sliding groove, a first reset spring, a guide block, a pull rope and a guide wheel, the sliding groove of an inverted-T-shaped structure is arranged on the shell, the driving block is in sliding connection with the shell, the bottom end of the driving block slides with the sliding groove, the cross section of the bottom end of the driving block is of a regular hexagon structure, the latex pad with an arc accommodating cavity is arranged on the driving block, the guide block in sliding connection is arranged on three corners of the sliding groove, the guide block is in sliding connection with the shell, the first reset spring is arranged between the guide block and the shell, the first reset spring is arranged at one end of the guide block, which is deviated from the driving block, one end of the pull rope penetrates through the shell and extends to the guide block, the guide wheel for guiding the pull rope is arranged on the shell, wherein the ends of the two symmetrical pull ropes penetrate through the containing box and are wound on the guide shaft.
Specifically, fixed knot constructs including mount, spacing spring and accomodates the hole, the mount with sliding connection between the shell, another one the end fixing of stay cord is in the mount, the mount deviates from the one end of stay cord with be equipped with between the shell spacing spring, the equidistance is equipped with a plurality ofly and is used for fixing on the mount storage structure accomodate the hole.
Specifically, the storage structure includes end cover, one-way admission valve, a storage cylinder, sliding sleeve, slide bar, second reset spring, piston and rubber tube for the cylinder structure of storage digestion liquid medicine the storage cylinder with the mount block, the bottom block of storage cylinder has the rubber tube, the end cover with threaded connection between the storage cylinder, be equipped with one-way admission valve on the end cover, the bottom of end cover is equipped with and extends to the inside of storage cylinder the sliding sleeve, the slide bar with sliding connection between the sliding sleeve, the slide bar with be equipped with between the sliding sleeve second reset spring, the slide bar deviates from the one end of sliding sleeve is equipped with sliding connection the piston, the piston with sliding connection between the storage cylinder.
Specifically, the dropping structure comprises a stop block, a fifth reset spring, a first pressurizing chamber and a diversion trench, the stop block is in sliding connection with the storage cylinder, the fifth reset spring is arranged between the stop block and the storage cylinder, the first pressurizing chamber is arranged between the stop block and the storage cylinder, and an annular diversion trench which is communicated with the first pressurizing chamber is arranged between the storage cylinder and the rubber pipe.
Specifically, the second drive structure includes briquetting, gasbag, transmission stopper, locker room, third reset spring, fourth reset spring and sealing plug, the briquetting with rotate between the storage cylinder and be connected, the briquetting with be equipped with between the storage cylinder the gasbag, the bottom of storage cylinder is equipped with sliding connection the transmission stopper, the transmission stopper with the storage cylinder encloses into two the locker room, one of gasbag and bottom the locker room switches on, the transmission stopper with be equipped with between the storage cylinder third reset spring, be equipped with on the storage cylinder with the slip direction vertically of transmission stopper the sealing plug, be equipped with on the sealing plug with the through-hole that the rubber tube switched on, the sealing plug with be equipped with between the storage cylinder fourth reset spring.
Specifically, the pressure boost structure includes connecting rod, pivot, guide bar, connecting block, supercharging stopper and second supercharging chamber, the connecting rod is fixed in the transmission stopper, the connecting rod with sliding connection between the storage cylinder, the guide bar passes through the pivot with rotate between the storage cylinder and be connected, the one end of guide bar with roll connection between the connecting rod, the other end of guide bar with roll connection between the connecting block, the tip of connecting block is equipped with and has the through-hole supercharging stopper, supercharging stopper with enclose between the sealing plug into the second supercharging chamber.
Specifically, the use method of the injection device for culturing the hair follicle stem cells comprises the following steps:
s1: firstly, storing different types of digestive medicaments required by cell culture in a plurality of storage structures in a classified manner, and then clamping the storage structures with the fixed structures according to specifications and habits thereof;
s2: when the straws on the storage structure need to be replaced or discarded, the first driving structure drives the two storage structures to be opened, then the falling structure is driven to make the straws fall into one of the storage structures, and then a new disposable straw is taken out from the other storage structure;
s3: when different storage structures need to be used, the first driving structure drives the fixed structure and the shell to slide, so that the needed storage structures slide to a specified position;
s4: when the storage structure is used, the opening and closing of the storage structure are controlled through the second driving structure, and meanwhile, the pressurizing structure is driven through the second driving structure to pressurize and discharge liquid medicine to a culture dish.
The invention has the beneficial effects that:
(1) the invention relates to an injection device for culturing hair follicle stem cells and a using method thereof, wherein two containing structures for containing a suction pipe and medical waste are arranged on a shell, the containing structures are connected with a first driving structure on the shell, so that the first driving structure is conveniently driven by the elbow to conflict with the first driving structure, the first driving structure drives the two containing structures to automatically open and close, the hands can be more convenient to perform other operations, the containing structures are convenient to contain a rubber tube and the medical waste, and the efficiency and the quality of the culture of the hair follicle stem cells are greatly improved, namely: the elbow and the latex pad are firstly abutted, the top end of the latex pad is provided with an arc-shaped accommodating cavity which accords with the structure of a human body, the use comfort is improved, the fatigue is relieved, a plurality of rubber tubes and medical garbage boxes are respectively arranged inside two containing boxes according to the habit of an operator, when the rubber tubes are required to be replaced and the medical garbage is treated, the driving block slides along the direction of a connecting line parallel to the central line between the two containing boxes through the elbow, the bottom end of the driving block is of a regular hexagon structure, the driving block slides more smoothly inside the sliding groove, when the driving block is abutted against the corresponding guide block of the containing box, the guide block drives the first reset spring to compress, the distance between the guide block and the containing boxes is reduced, the torsion spring resets and drives the guide shaft to drive the cover plate to be automatically opened, meanwhile, the pull rope is wound on the guide shaft, when the driving block is loosened, first reset spring extension drive guide block slides with the spout, makes the guide block drive the stay cord and slides, and stay cord drive guiding axle rotates, and it is airtight that the guiding axle drives apron and containing box conflict, and torsion spring deformation, and then be convenient for control through the elbow and open and close between apron and the containing box, make the hand also more be convenient for carry out other operations, be convenient for take the rubber tube respectively and handle medical waste in the inside of two containing boxes, and then improved hair follicle stem cell culture's efficiency and quality greatly.
(2) The invention relates to an injection device for hair follicle stem cell culture and a use method thereof.A fixing structure for fixing a storage structure is arranged on a shell, the storage structure and the fixing structure are clamped, the fixing structure is connected with a first driving structure on the shell, so that the fixing structure is driven by the first driving structure to slide on the shell, the distance from the storage structure on the fixing structure to an operator is convenient to adjust, and the storage structure can be taken more conveniently and quickly, namely: when needs access storage structure, promote the drive block, the drive block is contradicted in the guide block that the mount corresponds, makes the compression of the first reset spring of guide block drive, and then makes the shrink of spacing spring drive the mount and slide on the shell, and the storage structure of the needs of being convenient for slides to the position of being convenient for to take of operating personnel hand under the drive of mount, and then makes the storage of storage structure convenient and fast more.
(3) According to the injection device for hair follicle stem cell culture and the use method thereof, the plurality of storage structures are clamped with the fixed structure, the arrangement of the storage structures is convenient for directly storing the consumed liquid medicine in the storage structures, the phenomenon that the storage bottle is opened repeatedly to make the consumed liquid medicine contact with air or even cause pollution is effectively avoided, namely: at first rotate the end cover, dismantle the end cover, the digestion liquid that the inside storage of storage cylinder needs one by one, then make and slide between piston and the storage cylinder, gaseous exhaust valve on the piston of passing through between piston and the storage cylinder discharges, then twist and move the end cover, threaded connection between end cover and the storage cylinder, the piston is contradicted with liquid, the inside at the sliding sleeve is accomodate to the slide bar, the compression of second reset spring, after the liquid medicine of the inside of storage cylinder reduces, gaseous inside through one-way admission valve entering end cover, make the atmospheric pressure at the both ends of piston invariable, slide between second reset spring extension drive slide bar and the sliding sleeve, the piston that makes the tip of slide bar is contradicted with the liquid medicine all the time, and then effectually prevented that liquid medicine and air contact from causing the pollution, make the injection of taking of liquid medicine convenient.
(4) When a new rubber tube needs to be replaced, the stopper is pressed, the stopper slides with the storage barrel, the stopper drives the fifth return spring to compress, the space of the first pressurizing chamber is reduced, air enters the diversion groove, the pressure of the diversion groove is increased, the air pressure is higher than the atmospheric pressure, the rubber tube is driven to be directly separated from the end of the storage barrel, then the new rubber tube is clamped with the end of the storage barrel, the stopper is released, the fifth return spring drives one end of the stopper, which deviates from the fifth return spring, to slide, the inner space of the first pressurizing chamber is increased, air enters the first pressurizing chamber through the diversion groove, the atmospheric pressure fixes the rubber tube, and the new rubber tube is replaced more conveniently and quickly.
(5) The invention relates to an injection device for hair follicle stem cell culture and a use method thereof, wherein a storage structure is provided with a second driving structure for sealing the storage structure, a pressurizing structure for pressurizing a digestion medicine is arranged inside the storage structure, and the second driving structure is connected with the pressurizing structure, so that the storage structure is conveniently sealed by the second driving structure when not used, and when the medicine liquid is needed to be used, the pressurizing structure is driven by the second driving structure to pressurize and drive the medicine liquid in the storage structure to be discharged, so that the use of the medicine liquid is more convenient and flexible, and the pollution of the medicine liquid is avoided, namely: when liquid medicine is needed to be used, the pressing block is pressed, the pressing block rotates with the storage cylinder, the pressing block abuts against the air bag, the air bag deforms, air in the air bag enters the inside of a storage chamber where a third reset spring is located, air pressure is increased to drive the transmission plug to slide with the storage cylinder, so that the space in the other storage chamber is reduced, pressure is increased to drive the sealing plug to slide with the storage cylinder, so that a fourth reset spring extends, a through hole in the sealing plug is communicated with a second pressurizing chamber, the transmission plug drives the connecting rod to slide, the connecting rod drives the guide rod to rotate around the rotating shaft, the other end of the guide rod abuts against the connecting block, the connecting block drives the pressurizing plug to pressurize the second pressurizing chamber, so that the liquid medicine in the second pressurizing chamber is discharged from the rubber pipe, when the liquid medicine is not used, the air bag resets, so that the volume of the storage chamber where the third reset spring is located, and then make the volume increase of another locker room, under the effect of atmospheric pressure and fourth reset spring's reset force, make the sealing plug slide and seal up the second pumping chamber, the transmission stopper drives the connecting rod and resets, the connecting rod drives the guide bar and resets, the guide bar drives the boosting stopper through the connecting block and resets, the inside through-hole that makes the inside liquid medicine of storage cylinder on the boosting stopper discharges to the inside of second pumping chamber, and then make the use of liquid medicine convenient more nimble, the pollution of liquid medicine has been avoided simultaneously.
Drawings
The invention is further illustrated with reference to the following figures and examples.
FIG. 1 is a schematic structural diagram of a preferred embodiment of the injection device and the method for culturing hair follicle stem cells provided by the invention;
FIG. 2 is a schematic view of a connection structure of the first driving structure and the housing shown in FIG. 1;
FIG. 3 is an enlarged view of portion A of FIG. 1;
FIG. 4 is a schematic structural view of the receiving structure shown in FIG. 3;
FIG. 5 is a schematic diagram of a connection structure between the memory structure shown in FIG. 1 and a second driving structure;
FIG. 6 is an enlarged view of the portion B shown in FIG. 5;
FIG. 7 is an enlarged view of section C of FIG. 5;
fig. 8 is a flow chart of the method for using the injection device for culturing the hair follicle stem cells.
In the figure: 1. a receiving structure, 11, a cover plate, 12, a receiving box, 13, a guide shaft, 14, a torsion spring, 2, a first driving structure, 21, a driving block, 22, an emulsion pad, 23, a chute, 24, a first return spring, 25, a guide block, 26, a pull rope, 27, a guide wheel, 3, a housing, 4, a fixing structure, 41, a fixing frame, 42, a limiting spring, 43, a receiving hole, 5, a storage structure, 51, an end cover, 52, a one-way air inlet valve, 53, a storage cylinder, 54, a sliding sleeve, 55, a sliding rod, 56, a second return spring, 57, a piston, 58, a rubber tube, 6, a second driving structure, 61, a pressing block, 62, an air bag, 63, a transmission plug, 64, a storage chamber, 65, a third return spring, 66, a fourth return spring, 67, a sealing plug, 7, a dropping structure, 71, a stop, 72, a fifth return spring, 73, a first pressurizing chamber, 74, a diversion trench, 8. pressurization structure, 81, connecting rod, 82, pivot, 83, guide bar, 84, connecting block, 85, pressure boost stopper, 86, second plenum chamber.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
As shown in fig. 1 to 7, an injection device for culturing hair follicle stem cells provided by the invention comprises a receiving structure 1, a first driving structure 2, a housing 3, a fixing structure 4, a storage structure 5, a second driving structure 6, an exfoliation structure 7 and a pressurizing structure 8; the housing 3 is provided with two receiving structures 1 for receiving a straw and medical waste, the housing 3 is provided with a fixing structure 4 for fixing the storage structure 5, the storage structure 5 is clamped with the fixing structure 4, and the fixing structure 4 and the receiving structures 1 are both connected to the first driving structure 2 on the housing 3; the side wall of the storage structure 5 for storing the digestive medicament is provided with the falling structure 7, the storage structure 5 is provided with a second driving structure 6 for sealing the storage structure 5, the inside of the storage structure 5 is provided with a pressurizing structure 8 for pressurizing the digestive medicament, and the second driving structure 6 is connected with the pressurizing structure 8.
Specifically, the containing structure 1 includes a cover plate 11, a containing box 12, a guide shaft 13 and a torsion spring 14, two symmetrically arranged containing boxes 12 are arranged at the top end of the housing 3, the guide shaft 13 is connected to the containing box 12 and the cover plate 11 in a penetrating manner, the guide shaft 13 is rotatably connected with the containing boxes 12, the guide shaft 13 is fixed to the cover plate 11, one end of the torsion spring 14 is fixed to the containing box 12, and the other end of the torsion spring 14 is fixed to the guide shaft 13.
Specifically, the first driving structure 2 includes a driving block 21, a latex pad 22, a sliding groove 23, a first return spring 24, a guide block 25, a pull rope 26 and a guide wheel 27, the sliding groove 23 of the inverted T-shaped structure is arranged on the housing 3, the driving block 21 is slidably connected with the housing 3, the bottom end of the driving block 21 slides between the sliding groove 23, the cross section of the bottom end of the driving block 21 is of a regular hexagon structure, the latex pad 22 with an arc-shaped accommodating cavity is arranged on the driving block 21, the guide blocks 25 slidably connected with three corners of the sliding groove 23 are arranged on the three corners of the sliding groove 23, the guide blocks 25 are slidably connected with the housing 3, the first return spring 24 is arranged between the guide blocks 25 and the housing 3, the first return spring 24 is arranged at one end of the guide block 25 deviating from the driving block 21, one end of the pull rope 26 runs through the housing 3 and extends to the guide blocks 25, the guide wheel 27 for guiding the pull rope 26 is arranged on the outer shell 3, wherein the ends of two symmetrical pull ropes 26 penetrate through the containing box 12 and are wound on the guide shaft 13; be equipped with two on the shell 3 and be used for accomodating straw and medical waste accomodate structure 1, accomodate structure 1 connect in on the shell 3 first drive structure 2, and then be convenient for contradict the drive through the elbow first drive structure 2 makes first drive structure 2 drives two accomodate structure 1 automatic opening and closing makes the hand also can be more convenient for carry out other operations, the setting of accomodating structure 1 is convenient for accomodate rubber tube 58 and medical waste, and then has improved the efficiency and the quality of hair follicle stem cell culture greatly, promptly: firstly, an elbow is abutted against the latex pad 22, an arc-shaped accommodating cavity is arranged at the top end of the latex pad 22, the structure of the latex pad conforms to the structure of a human body, the use comfort is improved, the fatigue is relieved, the rubber tubes 58 and the medical garbage can are respectively arranged inside the two containing boxes 12 according to the habit of an operator, when the rubber tubes 58 need to be replaced and the medical garbage is treated, the driving block 21 slides along the direction of a connecting line parallel to the central line between the two containing boxes 12 through the elbow, the bottom end of the driving block 21 is of a regular hexagon structure, the driving block 21 further slides more smoothly inside the sliding groove 23, when the driving block 21 is abutted against the guide block 25 corresponding to the containing box 12, the guide block 25 drives the first return spring 24 to be compressed, and the distance between the guide block 25 and the containing box 12 is reduced, and then the torsion spring 14 is reset to drive the guide shaft 13 to drive the cover plate 11 to be automatically opened, meanwhile, the pull rope 26 is wound on the guide shaft 13, when the driving block 21 is loosened, the first reset spring 24 extends to drive the guide block 25 and the chute 23 to slide, so that the guide block 25 drives the pull rope 26 to slide, the pull rope 26 drives the guide shaft 13 to rotate, the guide shaft 13 drives the cover plate 11 to be abutted and sealed with the containing box 12, the torsion spring 14 deforms, and further, the opening and closing between the cover plate 11 and the containing box 12 are controlled through the elbow, so that the hands can be more convenient to carry out other operations, the rubber tube 58 and medical waste treatment can be respectively taken from the inside of the two containing boxes 12, and the efficiency and the quality of hair follicle stem cell culture are greatly improved.
Specifically, the fixing structure 4 includes a fixing frame 41, a limiting spring 42 and a receiving hole 43, the fixing frame 41 is connected with the outer shell 3 in a sliding manner, an end portion of the other pulling rope 26 is fixed to the fixing frame 41, the limiting spring 42 is arranged between one end of the fixing frame 41, which is away from the pulling rope 26, and the outer shell 3, and the fixing frame 41 is equidistantly provided with a plurality of receiving holes 43 for fixing the storage structure 5; be equipped with on shell 3 and be used for fixing storage structure 5 fixed knot constructs 4, storage structure 5 with block between the fixed knot constructs 4, just fixed knot constructs 4 connect in on the shell 3 first drive structure 2, and then be convenient for pass through first drive structure 2 drive fixed knot constructs 4 is in slide on the shell 3, be convenient for adjust on the fixed knot constructs 4 storage structure 5 is apart from operating personnel's distance, and then makes and take storage structure 5 convenient and fast more, promptly: when needs access during storage structure 5, promote drive block 21, drive block 21 contradict in mount 41 corresponds guide block 25 makes guide block 25 drive first reset spring 24 compresses, and then makes spacing spring 42 contracts and drives mount 41 is in slide on the shell 2, be convenient for need storage structure 5 is in slide to the operating personnel hand position of being convenient for to take under mount 41's the drive, and then make storage structure 5's storage is convenient and fast more.
Specifically, the storage structure 5 comprises an end cover 51, a one-way air inlet valve 52, a storage cylinder 53, a sliding sleeve 54, a sliding rod 55, a second return spring 56, a piston 57 and a rubber tube 58, the storage cylinder 53 with a cylindrical structure for storing the digestion liquid medicine is clamped with the fixing frame 41, the rubber tube 58 is clamped at the bottom end of the storage cylinder 53, the end cover 51 is in threaded connection with the storage cylinder 53, the end cover 51 is provided with a one-way air inlet valve 52, the bottom end of the end cover 51 is provided with the sliding sleeve 54 extending to the interior of the storage cylinder 53, the sliding rod 55 is connected with the sliding sleeve 54 in a sliding manner, the second return spring 56 is arranged between the sliding rod 55 and the sliding sleeve 54, one end of the sliding rod 55, which is far away from the sliding sleeve 54, is provided with the piston 57 in sliding connection, and the piston 57 is in sliding connection with the storage cylinder 53; a plurality of storage structure 5 with 4 block of fixed knot constructs, storage structure 5 set up be convenient for consume the direct storage of liquid medicine in storage structure 5's inside, the effectual reciprocal storage bottle of opening of having avoided makes the liquid medicine of disappearing and air contact cause the pollution even, promptly: firstly, the end cap 51 is rotated, the end cap 51 is removed, the required digestive juice is stored in the storage cylinder 53 one by one, then the piston 57 and the storage cylinder 53 are slid, the gas between the piston 57 and the storage cylinder 53 is discharged through the one-way exhaust valve on the piston 57, then the end cap 51 is screwed, the end cap 51 is screwed with the storage cylinder 53, the piston 57 is abutted with the liquid, the sliding rod 55 is accommodated in the sliding sleeve 54, the second return spring 56 is compressed, after the juice in the storage cylinder 53 is reduced, the gas enters the end cap 51 through the one-way air inlet valve 52, the gas pressure at both ends of the piston 57 is constant, the second return spring 56 is extended to drive the sliding between the sliding rod 55 and the sliding sleeve 54, and the piston 57 at the end of the sliding rod 55 is always abutted with the juice, thereby effectively preventing the liquid medicine from contacting with the air to cause pollution and enabling the taking and injection of the liquid medicine to be more convenient and faster.
Specifically, the dropping structure 7 includes a stopper 71, a fifth return spring 72, a first pressurizing chamber 73 and a diversion trench 74, the stopper 71 is slidably connected with the storage cylinder 53, the fifth return spring 72 is disposed between the stopper 71 and the storage cylinder 53, the first pressurizing chamber 73 is disposed between the stopper 71 and the storage cylinder 53, and the diversion trench 74 which leads to the first pressurizing chamber 73 is disposed between the storage cylinder 53 and the rubber tube 58 in an annular shape; the side wall of the storage structure 5 for storing the digestive medicament is provided with the falling structure 7, so that the rubber tube 58 is replaced more conveniently and quickly, namely: when the rubber tube 58 needs to be replaced with a new one, the stopper 71 is pressed, the stopper 71 slides with the storage cylinder 53, the stopper 71 drives the fifth return spring 72 to compress, the space of the first pressurizing chamber 73 is reduced, the gas enters the guide groove 74, the pressure of the guiding groove 74 is increased to make the air pressure be greater than the atmospheric pressure, thereby facilitating to drive the rubber tube 58 to be directly separated from the end of the storage cylinder 53, then, a new rubber tube 58 is engaged with the end of the storage cylinder 53, the stopper 71 is released, the fifth return spring 72 drives one end of the stopper 71 away from the fifth return spring 72 to slide, so that the inner space of the first pressurizing chamber 73 becomes larger, air enters the inside of the first pressurizing chamber 73 through the diversion groove 74, thereby fixing the rubber tube 58 by atmospheric pressure and further making the replacement of the rubber tube 58 more convenient and faster.
Specifically, the second driving structure 6 comprises a pressure block 61, an air bag 62, a transmission plug 63, a storage chamber 64, a third return spring 65, a fourth return spring 66 and a sealing plug 67, the pressing block 61 is rotatably connected with the storage cylinder 53, the air bag 62 is arranged between the pressing block 61 and the storage cylinder 53, the bottom end of the storage cylinder 53 is provided with the transmission plug 63 which is connected in a sliding way, the transmission plug 63 and the storage cylinder 53 enclose two storage chambers 64, the air bag 62 is communicated with one of the storage chambers 64 at the bottom end, the third return spring 65 is arranged between the transmission plug 63 and the storage cylinder 53, the storage cylinder 53 is provided with the sealing plug 67 perpendicular to the sliding direction of the transmission plug 63, a through hole communicated with the rubber tube 58 is formed in the sealing plug 67, and the fourth return spring 66 is arranged between the sealing plug 67 and the storage cylinder 53.
Specifically, the pressurizing structure 8 includes a connecting rod 81, a rotating shaft 82, a guide rod 83, a connecting block 84, a pressurizing plug 85 and a second pressurizing chamber 86, the connecting rod 81 is fixed to the transmission plug 63, the connecting rod 81 is slidably connected with the storage cylinder 53, the guide rod 83 is rotatably connected with the storage cylinder 53 through the rotating shaft 82, one end of the guide rod 83 is connected with the connecting rod 81 in a rolling manner, the other end of the guide rod 83 is connected with the connecting block 84 in a rolling manner, the end of the connecting block 84 is provided with the pressurizing plug 85 with a through hole, and the second pressurizing chamber 86 is enclosed between the pressurizing plug 85 and the sealing plug 67; be equipped with on storage structure 5 and be used for right storage structure 5 seals second drive structure 6, storage structure 5's inside is equipped with and is used for carrying out the pressure boost to the digestion medicament pressure boost structure 8, just second drive structure 6 connect in pressure boost structure 8, and then be convenient for pass through when not using second drive structure 6 is right storage structure 5 seals, when needs use the liquid medicine, through second drive structure 6 drives pressure boost structure 8 pressure boost drive the liquid medicine of storage structure 5's inside is discharged, and then makes the use of liquid medicine convenient more nimble, has avoided the pollution of liquid medicine simultaneously, promptly: when the liquid medicine needs to be used, the pressing block 61 is pressed, the pressing block 61 and the storage cylinder 53 rotate, the pressing block 61 abuts against the air bag 62, the air bag 62 deforms, air in the air bag 62 enters the storage chamber 64 where the third return spring 65 is located, air pressure is increased to drive the transmission plug 63 and the storage cylinder 53 to slide, so that the space in the other storage chamber 64 is reduced, pressure is increased to drive the sealing plug 67 and the storage cylinder 53 to slide, the fourth return spring 66 extends, the through hole in the sealing plug 67 is communicated with the second pressurizing chamber 86, the transmission plug 63 drives the connecting rod 81 to slide, the connecting rod 81 drives the guide rod 83 to rotate around the rotating shaft 82, the other end of the guide rod 83 abuts against the connecting block 84, and the connecting block 84 drives the pressurizing plug 85 to pressurize the second pressurizing chamber 86, when the liquid medicine is not used, the airbag 62 is reset, so that the volume of the storage chamber 64 in which the third return spring 65 is located is reduced, the third return spring 65 drives the transmission plug 63 to reset, so that the volume of the other storage chamber 64 is increased, the sealing plug 67 slides to seal the second pressurizing chamber 86 under the action of atmospheric pressure and the reset force of the fourth return spring 66, the transmission plug 63 drives the connecting rod 81 to reset, the connecting rod 81 drives the guide rod 83 to reset, the guide rod 83 drives the pressurizing plug 85 to reset through the connecting block 84, so that the liquid medicine in the storage cylinder 53 is discharged to the inside of the second pressurizing chamber 86 through the through hole on the pressurizing plug 85, and the use of the liquid medicine is more convenient and flexible, meanwhile, the pollution of the liquid medicine is avoided.
Specifically, referring to fig. 8, fig. 8 is a flowchart of a method for using the injection device for hair follicle stem cell culture according to the present invention, wherein the method for using the injection device for hair follicle stem cell culture comprises the following steps:
s1: firstly, storing different types of digestive medicaments required by cell culture in a plurality of storage structures 5 in a classified manner, and then clamping the storage structures 5 with the fixed structure 4 according to specifications and habits thereof; rotating the end cap 51, detaching the end cap 51, storing the required digestive juice one by one in the storage cylinder 53, then making the piston 57 slide between the storage cylinder 53, the gas between the piston 57 and the storage cylinder 53 is discharged through the one-way exhaust valve on the piston 57, then screwing the end cap 51, the end cap 51 is in threaded connection with the storage cylinder 53, the piston 57 is in contact with the liquid, the sliding rod 55 is stored in the sliding sleeve 54, the second return spring 56 is compressed, after the juice in the storage cylinder 53 is reduced, the gas enters the end cap 51 through the one-way intake valve 52, so that the gas pressure at the two ends of the piston 57 is constant, the second return spring 56 extends to drive the sliding rod 55 to slide between the sliding sleeve 54, so that the piston 57 at the end of the sliding rod 55 is in contact with the juice all the time, thereby effectively preventing the pollution caused by the contact of the liquid medicine and the air and enabling the taking and the injection of the liquid medicine to be more convenient and faster;
s2: when the straws on the storage structure 5 need to be replaced or discarded, the first driving structure 2 drives the two storage structures 1 to be opened, then the falling structure 7 is driven to make the straws fall into one of the storage structures 1, and then a new disposable straw is taken out from the other storage structure 1; firstly, an elbow is abutted against the latex pad 22, an arc-shaped accommodating cavity is arranged at the top end of the latex pad 22, the structure of the latex pad conforms to the structure of a human body, the use comfort is improved, the fatigue is relieved, the rubber tubes 58 and the medical garbage can are respectively arranged inside the two containing boxes 12 according to the habit of an operator, when the rubber tubes 58 need to be replaced and the medical garbage is treated, the driving block 21 slides along the direction of a connecting line parallel to the central line between the two containing boxes 12 through the elbow, the bottom end of the driving block 21 is of a regular hexagon structure, the driving block 21 further slides more smoothly inside the sliding groove 23, when the driving block 21 is abutted against the guide block 25 corresponding to the containing box 12, the guide block 25 drives the first return spring 24 to be compressed, and the distance between the guide block 25 and the containing box 12 is reduced, the torsion spring 14 is reset to drive the guide shaft 13 to drive the cover plate 11 to open automatically, when a new rubber tube 58 needs to be replaced, the stopper 71 is pressed, the stopper 71 slides with the storage cylinder 53, the stopper 71 drives the fifth reset spring 72 to compress, the space of the first pressurizing chamber 73 is reduced, air enters the diversion groove 74, the pressure of the diversion groove 74 is increased, the air pressure is greater than the atmospheric pressure, the rubber tube 58 is driven to be directly separated from the end of the storage cylinder 53, then the new rubber tube 58 is clamped with the end of the storage cylinder 53, the stopper 71 is released, the fifth reset spring 72 drives one end of the stopper 71 departing from the fifth reset spring 72 to slide, the internal space of the first pressurizing chamber 73 is enlarged, and air enters the interior of the first pressurizing chamber 73 through the diversion groove 74, the rubber tube 58 is fixed by atmospheric pressure, and the replacement of the rubber tube 58 is more convenient and faster; meanwhile, the pulling rope 26 is wound around the guide shaft 13, when the driving block 21 is loosened, the first return spring 24 extends to drive the guide block 25 and the chute 23 to slide, so that the guide block 25 drives the pulling rope 26 to slide, the pulling rope 26 drives the guide shaft 13 to rotate, the guide shaft 13 drives the cover plate 11 and the containing box 12 to be abutted and sealed, the torsion spring 14 deforms, opening and closing between the cover plate 11 and the containing box 12 are controlled through the elbow, other operations can be performed on the hand, the rubber tube 58 and medical waste treatment can be respectively taken from the two containing boxes 12, and the efficiency and quality of hair follicle stem cell culture are greatly improved;
s3: when different storage structures 5 need to be used, the first driving structure 2 drives the fixed structure 4 and the shell 3 to slide, so that the needed storage structures 5 slide to a specified position; when the storage structure 5 needs to be accessed, the driving block 21 is pushed, the driving block 21 abuts against the guide block 25 corresponding to the fixing frame 41, the guide block 25 drives the first return spring 24 to compress, and then the limiting spring 42 contracts to drive the fixing frame 41 to slide on the shell 2, so that the storage structure 5 needing to be accessed can slide to a position where an operator can conveniently take the storage structure by the hand of the operator under the driving of the fixing frame 41, and the storage of the storage structure 5 is more convenient and faster;
s4: when the storage structure 5 is used, the second driving structure 6 is used for controlling the opening and closing of the storage structure 5, and the second driving structure 6 is used for driving the pressurizing structure 8 to pressurize and discharge liquid medicine to a culture dish; when the liquid medicine needs to be used, the pressing block 61 is pressed, the pressing block 61 and the storage cylinder 53 rotate, the pressing block 61 abuts against the air bag 62, the air bag 62 deforms, air in the air bag 62 enters the storage chamber 64 where the third return spring 65 is located, air pressure is increased to drive the transmission plug 63 and the storage cylinder 53 to slide, so that the space in the other storage chamber 64 is reduced, pressure is increased to drive the sealing plug 67 and the storage cylinder 53 to slide, the fourth return spring 66 extends, the through hole in the sealing plug 67 is communicated with the second pressurizing chamber 86, the transmission plug 63 drives the connecting rod 81 to slide, the connecting rod 81 drives the guide rod 83 to rotate around the rotating shaft 82, the other end of the guide rod 83 abuts against the connecting block 84, and the connecting block 84 drives the pressurizing plug 85 to pressurize the second pressurizing chamber 86, when the liquid medicine is not used, the airbag 62 is reset, so that the volume of the storage chamber 64 in which the third return spring 65 is located is reduced, the third return spring 65 drives the transmission plug 63 to reset, so that the volume of the other storage chamber 64 is increased, the sealing plug 67 slides to seal the second pressurizing chamber 86 under the action of atmospheric pressure and the reset force of the fourth return spring 66, the transmission plug 63 drives the connecting rod 81 to reset, the connecting rod 81 drives the guide rod 83 to reset, the guide rod 83 drives the pressurizing plug 85 to reset through the connecting block 84, so that the liquid medicine in the storage cylinder 53 is discharged to the inside of the second pressurizing chamber 86 through the through hole on the pressurizing plug 85, and the use of the liquid medicine is more convenient and flexible, meanwhile, the pollution of the liquid medicine is avoided.
When the invention is used, firstly, the end cover 51 is rotated, the end cover 51 is detached, the required digestive liquid medicine is stored into the storage cylinder 53 one by one, then the piston 57 and the storage cylinder 53 are made to slide, the gas between the piston 57 and the storage cylinder 53 is exhausted through the one-way exhaust valve on the piston 57, then the end cover 51 is screwed, the end cover 51 is in threaded connection with the storage cylinder 53, the piston 57 is abutted against the liquid, the sliding rod 55 is accommodated in the sliding sleeve 54, the second return spring 56 is compressed, after the liquid medicine in the storage cylinder 53 is reduced, the gas enters the end cover 51 through the one-way air inlet valve 52, the gas pressure at the two ends of the piston 57 is constant, the second return spring 56 is extended to drive the sliding rod 55 and the sliding sleeve 54 to slide, the piston 57 at the end of the sliding rod 55 is always abutted against the liquid medicine, further, the pollution caused by the contact between the liquid medicine and the air is, the elbow is abutted against the latex pad 22, the top end of the latex pad 22 is provided with an arc-shaped accommodating cavity which accords with the structure of a human body, the use comfort is improved, the fatigue is relieved, the rubber tubes 58 and the medical garbage can are respectively arranged in the two containing boxes 12 according to the habit of an operator, when the rubber tubes 58 are required to be replaced and the medical garbage is treated, the driving block 21 slides along the direction of a connecting line parallel to the central line between the two containing boxes 12 through the elbow, the bottom end of the driving block 21 is of a regular hexagon structure, the driving block 21 slides more smoothly in the chute 23, when the driving block 21 is abutted against the guide block 25 corresponding to the containing box 12, the guide block 25 drives the first reset spring 24 to be compressed, the distance between the guide block 25 and the containing boxes 12 is reduced, the torsion spring 14 is reset to drive the guide shaft 13 to drive the cover plate 11 to be automatically opened, when a new rubber tube 58 is required to be replaced, the stopper 71 is pressed, the stopper 71 slides with the storage barrel 53, the stopper 71 drives the fifth return spring 72 to compress, the space of the first pressurizing chamber 73 is reduced, air enters the diversion groove 74, the pressure of the diversion groove 74 is increased, the air pressure is greater than the atmospheric pressure, the rubber tube 58 is conveniently driven to be directly separated from the end part of the storage barrel 53, then the new rubber tube 58 is clamped with the end part of the storage barrel 53, the stopper 71 is released, the fifth return spring 72 drives one end of the stopper 71, which is far away from the fifth return spring 72, to slide, the inner space of the first pressurizing chamber 73 is enlarged, air enters the inner part of the first pressurizing chamber 73 through the diversion groove 74, the atmospheric pressure fixes the rubber tube 58, and replacement of the new rubber tube 58 is more convenient and rapid; meanwhile, the pull rope 26 is wound on the guide shaft 13, when the driving block 21 is loosened, the first return spring 24 extends to drive the guide block 25 and the chute 23 to slide, so that the guide block 25 drives the pull rope 26 to slide, the pull rope 26 drives the guide shaft 13 to rotate, the guide shaft 13 drives the cover plate 11 to abut against and seal with the containing boxes 12, the torsion spring 14 deforms, opening and closing between the cover plate 11 and the containing boxes 12 are controlled through the elbows, hands can be operated more conveniently, the rubber tube 58 and medical waste can be taken from the two containing boxes 12 respectively, the efficiency and quality of hair follicle stem cell culture are greatly improved, when the storage structure 5 needs to be accessed, the driving block 21 is pushed, the driving block 21 abuts against the guide block 25 corresponding to the fixing frame 41, the guide block 25 drives the first return spring 24 to compress, and the limiting spring 42 contracts to drive the fixing frame 41 to slide on the housing 2, when liquid medicine needs to be used, the pressing block 61 is pressed, the pressing block 61 and the storage cylinder 53 rotate, the pressing block 61 abuts against the air bag 62, the air bag 62 deforms, air in the air bag 62 enters the inside of the storage chamber 64 where the third return spring 65 is located, air pressure is increased to drive the transmission plug 63 and the storage cylinder 53 to slide, the space in the other storage chamber 64 is reduced, the pressure is increased to drive the sealing plug 67 and the storage cylinder 53 to slide, the fourth return spring 66 extends, the through hole in the sealing plug 67 is communicated with the second pressurizing chamber 86, the transmission plug 63 drives the connecting rod 81 to slide, the connecting rod 81 drives the guide rod 83 to rotate around the rotating shaft 82, and the other end of the guide rod 83 abuts against the connecting block 84, the connecting block 84 drives the pressurizing plug 85 to pressurize the second pressurizing chamber 86, so that the liquid medicine in the second pressurizing chamber 86 is discharged from the rubber tube 58, when the liquid medicine is not used, the air bag 62 is reset, thereby reducing the volume of the storage chamber 64 in which the third return spring 65 is positioned, the third return spring 65 drives the transmission plug 63 to return, thereby increasing the volume of the other storage chamber 64, under the effect of atmospheric pressure and fourth reset spring 66's restoring force, make sealing plug 67 slide and seal up second pumping chamber 86, transmission stopper 63 drives connecting rod 81 and resets, connecting rod 81 drives guide bar 83 and resets, guide bar 83 drives boosting stopper 85 through connecting block 84 and resets, the inside liquid medicine that makes storage cylinder 53 discharges to second pumping chamber 86 through the through-hole on boosting stopper 85, and then make the use of liquid medicine convenient more nimble, the pollution of liquid medicine has been avoided simultaneously.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.

Claims (9)

1. An injection device for culturing hair follicle stem cells is characterized by comprising a containing structure (1), a first driving structure (2), a shell (3), a fixing structure (4), a storage structure (5), a second driving structure (6), an off-body structure (7) and a pressurizing structure (8); the storage structure (1) for storing the suction tube and the medical waste is arranged on the shell (3), the fixing structure (4) for fixing the storage structure (5) is arranged on the shell (3), the storage structure (5) is clamped with the fixing structure (4), and the fixing structure (4) and the storage structure (1) are both connected with the first driving structure (2) on the shell (3); the side wall of the storage structure (5) for storing the digestive medicament is provided with the falling structure (7), the storage structure (5) is provided with a second driving structure (6), the storage structure (5) is sealed, the inside of the storage structure (5) is provided with a pressurizing structure (8) for pressurizing the digestive medicament, and the second driving structure (6) is connected with the pressurizing structure (8).
2. The injection device for hair follicle stem cell culture according to claim 1, characterized in that: the storage structure (1) comprises a cover plate (11), a storage box (12), a guide shaft (13) and a torsion spring (14), wherein the two storage boxes (12) are symmetrically arranged at the top end of the shell (3), the guide shaft (13) is connected to the storage box (12) and the cover plate (11) in a penetrating mode, the guide shaft (13) is connected with the storage box (12) in a rotating mode, the guide shaft (13) is fixed to the cover plate (11), one end of the torsion spring (14) is fixed to the storage box (12), and the other end of the torsion spring (14) is fixed to the guide shaft (13).
3. The injection device for hair follicle stem cell culture according to claim 2, characterized in that: the first driving structure (2) comprises a driving block (21), a latex pad (22), a sliding groove (23), a first reset spring (24), a guide block (25), a pull rope (26) and a guide wheel (27), the sliding groove (23) of an inverted T-shaped structure is arranged on the shell (3), the driving block (21) is connected with the shell (3) in a sliding manner, the bottom end of the driving block (21) slides with the sliding groove (23), the cross section of the bottom end of the driving block (21) is of a regular hexagon structure, the latex pad (22) with an arc containing cavity is arranged on the driving block (21), the guide block (25) is arranged on three corners of the sliding groove (23) in a sliding manner, the guide block (25) is connected with the shell (3) in a sliding manner, the first reset spring (24) is arranged between the guide block (25) and the shell (3), the first return spring (24) is arranged at one end, deviating from the driving block (21), of the guide block (25), one end of the pull rope (26) penetrates through the shell (3) and extends to the guide block (25), the guide wheel (27) used for guiding the pull rope (26) is arranged on the shell (3), and the end portions of the two symmetrical pull ropes (26) penetrate through the storage box (12) and are wound on the guide shaft (13).
4. An injection device for hair follicle stem cell culture according to claim 3, characterized in that: fixed knot constructs (4) including mount (41), spacing spring (42) and accomodate hole (43), mount (41) with sliding connection between shell (3), another one the tip of stay cord (26) is fixed in mount (41), mount (41) deviate from the one end of stay cord (26) with be equipped with between shell (3) spacing spring (42), mount (41) equidistance is equipped with a plurality ofly and is used for fixing storage structure (5) accomodate hole (43).
5. The injection device for hair follicle stem cell culture according to claim 4, characterized in that: storage structure (5) are including end cover (51), one-way admission valve (52), a storage cylinder (53), sliding sleeve (54), slide bar (55), second reset spring (56), piston (57) and rubber tube (58) for the cylinder structure of storage digestion liquid medicine storage cylinder (53) with mount (41) block, the bottom block of storage cylinder (53) has rubber tube (58), end cover (51) with threaded connection between storage cylinder (53), be equipped with one-way admission valve (52) on end cover (51), the bottom of end cover (51) is equipped with and extends to the inside of storage cylinder (53) sliding sleeve (54), slide bar (55) with sliding connection between sliding sleeve (54), slide bar (55) with be equipped with between sliding sleeve (54) second reset spring (56), slide bar (55) deviate from the one end of sliding sleeve (54) is equipped with sliding connection piston (57) The piston (57) is connected with the storage cylinder (53) in a sliding mode.
6. The injection device for hair follicle stem cell culture according to claim 5, characterized in that: the dropping structure (7) comprises a stop block (71), a fifth reset spring (72), a first pressurizing chamber (73) and a diversion trench (74), the stop block (71) is in sliding connection with the storage cylinder (53), the fifth reset spring (72) is arranged between the stop block (71) and the storage cylinder (53), the first pressurizing chamber (73) is arranged between the stop block (71) and the storage cylinder (53), and an annular diversion trench (74) communicated with the first pressurizing chamber (73) is arranged between the storage cylinder (53) and the rubber tube (58).
7. The injection device for hair follicle stem cell culture according to claim 5, characterized in that: the second driving structure (6) comprises a pressing block (61), an air bag (62), a transmission plug (63), a storage chamber (64), a third return spring (65), a fourth return spring (66) and a sealing plug (67), the pressing block (61) is rotatably connected with the storage cylinder (53), the air bag (62) is arranged between the pressing block (61) and the storage cylinder (53), the transmission plug (63) is arranged at the bottom end of the storage cylinder (53) in sliding connection, the transmission plug (63) and the storage cylinder (53) are enclosed into two storage chambers (64), the air bag (62) is communicated with one storage chamber (64) at the bottom end, the third return spring (65) is arranged between the transmission plug (63) and the storage cylinder (53), the sealing plug (67) perpendicular to the sliding direction of the transmission plug (63) is arranged on the storage cylinder (53), the sealing plug (67) is provided with a through hole communicated with the rubber tube (58), and the fourth return spring (66) is arranged between the sealing plug (67) and the storage cylinder (53).
8. The injection device for hair follicle stem cell culture according to claim 7, characterized in that: the pressurizing structure (8) comprises a connecting rod (81), a rotating shaft (82), a guide rod (83), a connecting block (84), a pressurizing plug (85) and a second pressurizing chamber (86), the connecting rod (81) is fixed on the transmission plug (63), the connecting rod (81) is in sliding connection with the storage cylinder (53), the guide rod (83) is in rotating connection with the storage cylinder (53) through the rotating shaft (82), one end of the guide rod (83) is in rolling connection with the connecting rod (81), the other end of the guide rod (83) is in rolling connection with the connecting block (84), the pressurizing plug (85) with a through hole is arranged at the end of the connecting block (84), and the second pressurizing chamber (86) is formed between the pressurizing plug (85) and the sealing plug (67).
9. Use of an injection device for hair follicle stem cell culture according to any one of claims 1-8, characterized in that: the method comprises the following steps:
s1: firstly, storing different types of digestive medicaments required by cell culture in a plurality of storage structures (5) in a classified manner, and then clamping the storage structures (5) with the fixed structures (4) according to specifications and habits thereof;
s2: when the straws on the storage structure (5) need to be replaced or discarded, the first driving structure (2) drives the two storage structures (1) to be opened, then the falling structure (7) is driven to make the straws fall into one of the storage structures (1), and then a new disposable straw is taken out from the other storage structure (1);
s3: when different storage structures (5) need to be used, the first driving structure (2) drives the fixed structure (4) and the shell (3) to slide, so that the needed storage structures (5) slide to a specified position;
s4: when the storage structure (5) is used, the second driving structure (6) is used for controlling the storage structure (5) to be opened and closed, and meanwhile, the second driving structure (6) is used for driving the pressurizing structure (8) to pressurize and discharge liquid medicine to a culture dish.
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CN113611200A (en) * 2021-08-11 2021-11-05 中国人民解放军空军军医大学 Injection teaching instrument for medical nursing major

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CN102586177A (en) * 2012-02-15 2012-07-18 北京雍禾植发技术研究院 Culture method of hair follicle stem cell for treating baldness, as well as syringe
US20180221568A1 (en) * 2015-08-03 2018-08-09 Chongqing Beka Med, Inc. Injection device
CN110305944A (en) * 2019-06-26 2019-10-08 王中刚 A kind of cool-down method for hereditary tumor genetic test

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CN102586177A (en) * 2012-02-15 2012-07-18 北京雍禾植发技术研究院 Culture method of hair follicle stem cell for treating baldness, as well as syringe
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