CN111418648B - Essential oil antiseptic insect repellent for waxberries - Google Patents
Essential oil antiseptic insect repellent for waxberries Download PDFInfo
- Publication number
- CN111418648B CN111418648B CN202010115219.1A CN202010115219A CN111418648B CN 111418648 B CN111418648 B CN 111418648B CN 202010115219 A CN202010115219 A CN 202010115219A CN 111418648 B CN111418648 B CN 111418648B
- Authority
- CN
- China
- Prior art keywords
- essential oil
- waxberries
- insect repellent
- antiseptic
- plant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B7/00—Preservation or chemical ripening of fruit or vegetables
- A23B7/14—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
- A23B7/153—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of liquids or solids
- A23B7/154—Organic compounds; Microorganisms; Enzymes
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/24—Lauraceae [Laurel family], e.g. laurel, avocado, sassafras, cinnamon or camphor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/36—Rutaceae [Rue family], e.g. lime, orange, lemon, corktree or pricklyash
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B7/00—Preservation or chemical ripening of fruit or vegetables
- A23B7/14—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
- A23B7/144—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of gases, e.g. fumigation; Compositions or apparatus therefor
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Mycology (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Environmental Sciences (AREA)
- Biotechnology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Botany (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses an essential oil antiseptic insect repellent for waxberries, which is characterized by comprising the following components in percentage by weight: 1 to 30 percent of plant essential oil, 0.1 to 10 percent of Tween 80 and the balance of deionized water. The plant essential oil is preferably litsea cubeba essential oil, orange essential oil and borneol camphor essential oil, and the mass ratio of the plant essential oil to the borneol essential oil is 3:2:1. Experiments prove that the three plant essential oils are mixed according to a proper proportion to generate a synergistic effect, so that the functions are better realized. The essential oil in the essential oil anti-corrosion insect repellent belongs to food additives, is safe to waxberries and human bodies, and has small environmental pollution.
Description
Technical Field
The invention relates to the technical field of preservation, and particularly relates to an essential oil antiseptic insect repellent for waxberries.
Background
The waxberry is one of the special fruits in China, and has the reputation of being questioned for one thousand of gold. The waxberry fruits are bright in color, rich in juice, palatable in sweetness and sourness and high in nutritive value, and the waxberry fruits are just in a slack season when being mature, so that edible fresh fruits are provided for the market and are deeply popular with consumers. However, the red bayberries are easy to rot and not resistant to storage, and are very easy to attract insects (fruit flies), so that the corrosion prevention and insect expelling of the red bayberries are very important links in the production, processing and sale processes of the red bayberries. The existing anticorrosion and insect-repellent technology mainly comprises a physical method and a chemical method, the chemical method comprises medicament spraying, soaking treatment and the like, the physical preservation cost is high, and the taste of the waxberries is adversely affected, so that chemical reagents are frequently used in the existing anticorrosion and insect-repellent technology to carry out early-stage treatment on the waxberries, but the components of certain chemical reagents are harmful to the bodies, the drug resistance is easy to generate, and certain pollution is caused to the environment.
The compound essential oil preservative and insect repellent is prepared by mixing several essential oils according to different components, and the various raw materials have strong antibacterial activity and antioxidant activity under the combined action, so that the spoilage of the waxberries by microorganisms is inhibited, and the preservative and fresh-keeping effects of the fruits are achieved. Meanwhile, the essential oil has the functions of killing and expelling insects, and can better reduce the quantity of fruit flies and other insects in the waxberry. At present, no compound essential oil is used as a fresh-keeping insect repellent for the waxberries in the existing research.
Therefore, in order to solve the defects of the prior art, the invention provides the preservative and insect repellent which has good preservative and fresh-keeping effects and strong insect repelling effect, and is safe and environment-friendly for waxberries and human bodies.
Disclosure of Invention
The invention aims to provide an essential oil antiseptic insect repellent for waxberries, which can effectively reduce the spoilage and insect rate of the waxberries and improve the quality of the waxberries.
In order to achieve the purpose, the invention provides the following technical scheme:
an essential oil antiseptic insect repellent for waxberries, which is characterized by comprising the following components in percentage by weight: 1 to 30 percent of vegetable essential oil, 0.1 to 10 percent of Tween 80 and the balance of deionized water.
Further, the plant essential oil is one or a combination of a plurality of types of litsea cubeba essential oil, orange essential oil and borneol camphor essential oil.
Further, the plant essential oil is litsea cubeba essential oil, orange essential oil and borneol camphor essential oil, and the mass ratio of the plant essential oil to the borneol essential oil is 3:2:1. Experiments prove that the three plant essential oils are mixed according to a proper proportion to generate a synergistic effect (the synergistic effect refers to the effect of various common efforts which can be found in the decision of resource allocation, namely the effect of' 1+1> < 2 >), so that the functions are better realized. The litsea cubeba essential oil and the orange essential oil are combined to improve the antibacterial and insect-resistant performance, and when the litsea cubeba essential oil is the orange essential oil: when the borneol camphor essential oil = 3.
The invention also provides a pest expelling, corrosion preventing and fresh keeping method for the waxberries, which is characterized in that the essential oil corrosion preventing and pest expelling agent is adopted to fumigate the waxberries, and the fumigating time is 2-6 hours.
Another method for expelling parasites, preserving and preserving waxberries comprises uniformly spraying the essential oil-containing antiseptic and insect-repellent agent on waxberries 15 days, 10 days and 5 days before the waxberries are picked.
The plant essential oil is extracted by an extraction method or a distillation method commonly used in the field.
The invention has the following advantages and beneficial effects:
1. the essential oil in the essential oil anti-corrosive insect repellent belongs to food additives, is safe to waxberries and human bodies, and has little pollution to the environment.
2. The essential oil anti-corrosion insect repellent can quickly sterilize the surfaces of the waxberries, reduce the residue of active microorganisms and is not easy to rot.
3. The essential oil anti-corrosion insect repellent can greatly reduce the quantity of fruit fly larvae in the waxberries and improve the quality of the waxberries.
4. The compound essential oil has good spreading force, and can make the liquid medicine uniformly adhere to the surface of the waxberry, so that the preservation effect is uniform.
5. The plant essential oil selected by the invention has low cost, large production capacity in China and easy acquisition.
Drawings
Fig. 1 is a structural schematic diagram of a Y-shaped olfactometer.
Fig. 2 is a schematic representation of the effect of # 3 essential oil on survival (%) e.coli (a) and Salmonella (B).
Fig. 3 is a graph showing the extracellular relative conductivities (%) of e.coli (a) and Salmonella (B) after treatment with # 3 essential oil.
Fig. 4 is a schematic diagram of the nucleic acid leakage (OD 260) of e.coli (a) and Salmonella (B) after treatment with # 3 essential oil.
Coli and Salmonella variations of 3# essential oil treatment.
Fig. 6 shows the decrease in cell wall damage (AKP activity) after survival after treatment of e.coli with different concentrations of essential oil # 3: a, a control group under a 400-time optical microscope; b, C, D are e.coli after treatment with different concentrations of essential oil under a 400-fold fluorescence microscope (control, MIC, MBC), respectively.
Detailed Description
The invention discloses an essential oil antiseptic insect repellent for waxberries, which can be realized by properly improving parameters by referring to the content in the text. It is specifically noted that all such substitutions and modifications will be apparent to those skilled in the art and are intended to be included herein. While the invention has been described in terms of preferred embodiments, it will be apparent to those skilled in the art that the invention can be practiced and applied by modifying or appropriately combining the methods and applications described herein without departing from the spirit and scope of the invention.
In order to better illustrate the invention, reference will now be made to specific examples.
1. Zone of inhibition test
And (3) measuring the bacteriostatic activity of the essential oil by adopting an Oxford cup diffusion method. And (3) pouring the solid culture medium and the semi-solid bacterial liquid into the culture dish in sequence (after the solidification of the solid culture medium and the semi-solid bacterial liquid, adding 50 mu L of pure essential oil into the Oxford cup, and simultaneously taking sterile water as a blank control. The size of the zone of inhibition (minus the diameter of the Oxford cup) was determined after incubation at 37 ℃ for 24 h.
2. Determination of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC)
MIC and MBC of different combinations of essential oils were determined by 96-well plate method. Diluting with 10 times of ethanol to obtain 0.01% -1% essential oil, culturing control group with anhydrous ethanol at 37 deg.C overnight, and measuring Optical Density (OD) at 600nm with enzyme labeling instrument. Compared with the control group, the MIC is determined by the lowest concentration of the OD value which is smaller than that of the control hole. Dipping the essential oil and bacterial liquid mixture with multiple MIC concentration by using an inoculating loop, inoculating the mixture on an LB solid culture medium, culturing overnight at 37 ℃, and observing to obtain MBC with the lowest concentration without colony.
3. Drawing growth curve
Adding MIC, 3 × MIC essential oil and overnight culture solution into 96-well plate, culturing overnight at 37 deg.C, and recording OD at 0h,4h,8h,12h, 169h, 20h, and 24h 600 On the abscissa of time, OD 600 Growth curves were plotted for the ordinate.
4. Determination of the relative conductivity (REC)
The overnight cultured bacterial liquid was centrifuged at 4 ℃ and 6000G for 8min, washed with 5% glucose solution to obtain isotonic bacteria, treated with essential oil with MIC and MBC concentrations, and measured for conductivity at 37 ℃ every 2h, which was designated as L2. The conductivity was taken to be L1 for 0 h. L0 is the conductivity measured after boiling the bacterial solution in the glucose solution for 5 min. REC is calculated by the following formula: REC (%) = (L2-L1)/L0 × 100.
5. Determination of nucleic acid leakage
Centrifuging overnight culture liquid at 4 deg.C and 6000G for 8min, washing with phosphate buffer solution for 3 times, resuspending, adding composite essential oil with MIC and MBC concentrations, respectively, culturing at 37 deg.C for 24 hr, collecting supernatant every 4 hr, and measuring OD with enzyme labeling instrument 260 。
6. Determination of alkaline phosphatase (AKP) leakage
Adding 120 μ L of R1 solution, 30 μ L of R2 solution (R1, R2 solution is kit: nanjing institute of bioengineering), 3 μ L of washed bacterial suspension (method is the same as 1.6), adding MIC and MBC concentration compound essential oil, and immediately measuring OD with microplate reader 405 And the absorbance was measured again after 2 min.
7. Propidium Iodide (PI) analysis
The assay was performed using the PI staining kit method (BBI Life Sciences, consisting of fractions A and B). The component B buffer was diluted 10-fold with sterile water. Staining the treated bacterial liquid (the method is the same as 1.6), and standing and incubating for 5-30min. The stained bacterial suspension was prepared into a slide glass and the staining was observed with a fluorescence microscope.
8. Experiment for oviposition of fruit fly imago
The 9 essential oil combinations were diluted to 50-fold each with 1% tween 80 (20 mL/L concentration). 200 female fruit flies of 10-day-old age were collected, 100 flies of the control group and the experimental group, 5 flies per concentration, and 20 female fruit flies per tube. The drosophila corn culture medium used in the experiment is prepared by adding 200 μ L of 10% brilliant blue solution and 100 μ L of water on the surface of food as control group, and 100 μ L of essential oil diluent as experimental group. The fruit flies were placed on the treated food and placed in the medium, after 22h, the female flies were cleared and the number of fly eggs was counted under a microscope. Each essential oil combination was one experiment.
9. Selection and determination of fruit fly 'Y' -shaped tube behavior
A Y-shaped olfactometer is adopted to test the behavioral response of the fruit flies to an essential oil combination (litsea cubeba essential oil: orange essential oil: borneol camphor essential oil =3: 2). All the parts are connected by a silicone tube. The length of 2 side arms of the olfactometer is 15cm. The straight tube is 10cm long and the angle between the two arms is 70 (as shown in figure 1). During testing, one arm of the olfactometer is used as a treatment, and a test reagent is 3# essential oil combination. Respectively diluting 5, 10 and 15 μ L of 3# essential oil composition with 1mL of acetone to different concentrations, sucking the same amount of essential oil with a microsyringe, and uniformly dripping into 2cm container 2 An equal amount of acetone was dropped onto the filter paper as a control. And (3) placing the filter paper in the air, and respectively placing the treated filter paper and the control filter paper into corresponding olfactory testing arms after acetone is volatilized. The air flow in the test arm was 200mL/min. 20 female adults were introduced from the end of a straight tube of a "Y" type olfactometer. Behavioral responses of adults were observed and recorded for 10min each time. During the test, adults resting on the basal arm were considered unreacted. Using the above method, the response of females to different concentrations of the combination of 3# essential oils was determined separately. Treatment was performed 10 replicates per concentration.
And the directions of the two arms of the Y-shaped pipe are changed after each test. To eliminate the effect of geometric position on the behavior of the fruit fly. After each treatment, the entire olfactometer was immediately washed with 95% ethanol and dried in an oven at 100 ℃ for further use. All tests were conducted in a dedicated olfactory test chamber that was clean in air and relatively closed.
10. Preservation experiment for waxberry
(1) Selecting a plurality of waxberry parts with uniform size and no mechanical injury or pest damage, wherein each part has the mass of 1000g, and carrying out the following treatment. Filter paper of 5cm square was immersed with 10% (volume fraction) of each of 9 kinds of essential oil composition solutions, and water was used as a control. 4 pieces of filter paper are stuck to the bottom and the peripheral box walls of the fumigation box at certain intervals, and the waxberries are put into the porous placing box and then put into the corresponding fumigation box with a bracket, so that the direct contact between the surfaces of the waxberries and the liquid bacteriostatic agent is avoided. After sealing treatment, marking, fumigating in a 4 ℃ constant temperature climate box for 4h, subpackaging and marking the preservation boxes for each group of waxberries, and then placing in the 4 ℃ constant temperature climate box. And respectively measuring the hardness, the fruit yield and the maggot number of the fruit in the waxberry fruits of each treatment group at 5d and 10 d.
(2) Selecting a plurality of waxberry trees which have basically the same growth conditions and are in proper and similar growth environments. The control group was not treated at all and was allowed to grow normally; the experimental group utilized 1% 3# essential oil solution diluted to be uniformly sprayed on the waxberry trees for 3 times respectively 15 days, 10 days and 5 days before picking. After picking, the fruit is placed in a thermostatic climate box at 4 ℃, and the rot level and the number of the fruit fly maggots are measured every 5 days.
Example 1:
1# litsea cubeba essential oil
Example 2
2# orange essential oil
Example 3
3# litsea cubeba essential oil, orange essential oil, borneol camphor essential oil =3:2:1
Example 4
4# litsea cubeba essential oil and orange essential oil = 1: 1
Example 5
5# litsea cubeba essential oil and orange essential oil = 1: 2
Example 6
No. 6 borneol camphor essential oil
Example 7
7# orange essential oil to borneol camphor essential oil = 1: 2
Example 8
8# litsea cubeba essential oil and borneol camphor essential oil = 1: 1
Example 9
9# litsea cubeba essential oil and borneol camphor essential oil = 2:1
Analysis of results
1. Evaluation of antibacterial Activity
The antibacterial activity of the different combinations of essential oils is reflected by the zone diameters of inhibition of the essential oils against the test bacteria, fungi (table 1), MIC and MBC (table 2). As shown in Table 1, the compound essential oil has a good bacteriostatic effect on bacteria. Among different essential oil combinations, the 3# combination has better antibacterial effect on escherichia coli, salmonella, aspergillus and penicillium. The MIC and MBC results (Table 2) show that the 9 essential oil combinations have certain antibacterial activity on the strains, and the difference of the antibacterial effect between different essential oils and different test bacteria is possibly related to factors such as the water solubility of the essential oil, the cell envelope structure of bacteria and the like.
TABLE 1 inhibition zone diameter (mm) of different essential oil combinations for bacteria and fungi
TABLE 2 MIC (% v/v) and MBC (% v/v) for different combinations of essential oils
2. Effect of essential oils on bacterial and fungal Activity
On the basis of the experimental results of MIC and MBC measurements, the viable count changes of escherichia coli (a in fig. 2) and salmonella (B in fig. 2) within 24h under the action of 3# essential oil at different concentrations (control, MIC, MBC) were determined and growth curves were plotted. Under the action of the compound essential oil with different concentrations, the change trends of the bacterial activity of the two bacteria are basically consistent, namely the number of the viable bacteria of the bacteria liquid without essential oil treatment (Control) is gradually increased along with the time; the number of viable bacteria of the bacterial liquid treated by the essential oil (MIC) is almost kept unchanged; the number of viable bacteria in the essential oil treated (MBC) bacteria solution gradually decreases with time. The essential oil has obvious influence on the number of viable bacteria 16 hours before the bacterial liquid is cultured, and the number of viable bacteria of the bacterial liquid is basically stable after the bacterial liquid is cultured for 16 hours. It can be seen that essential oils have an effect on bacterial activity, and that a certain concentration of essential oil can reduce bacterial activity, while a high concentration of essential oil can inactivate bacteria.
3. Essential oil for destroying cell membrane permeability
The influence of the essential oil on the permeability of cell membranes is evaluated by measuring the change condition of relative conductivity of bacterial liquid before and after the composite essential oil with different concentrations (Control, MIC and MBC) is used for treating escherichia coli and salmonella. As shown in fig. 3, the conductivity of the control group hardly changed, and the conductivity of the bacteria solution exposed to the essential oil increased with time and increased with the increase of the concentration of the essential oil. The conductivity of the bacterial liquid is obviously increased within 0-2h, and after 2h, the conductivity is continuously increased but the increasing rate is reduced. The relative conductivity change trends of the two bacteria are approximately the same, and the conductivity at the concentration of MBC is greater than the conductivity at the concentration of MIC. Therefore, the essential oil has destructive effect on bacterial cell membranes, so that substances in cells are leaked in a large amount, and the conductivity is obviously improved.
4. Essential oil # 3 disrupts cell membrane integrity
Determination of OD in 24h of E.coli and Salmonella exposed to different concentrations (control, MIC, MBC) of essential oil 3# 260 FIG. 4 shows the OD of the cell suspension after 24h incubation when it was not exposed to essential oil (control) 260 The values did not change significantly; OD of bacteria liquid in 0-4h when exposed to essential oil (MIC, MBC) 260 The value rises rapidly, OD within 4h-24h 260 Increase continuously, but at a slower rate and under the action of MBC, OD 260 The variation amplitude of (2) is far larger than OD under the action of MIC 260 The amplitude of the change of (c). Therefore, the essential oil has a great damage effect on bacterial cell membranes, so that macromolecules such as nucleic acid in cells are released out of the cells, the use amount of the essential oil is increased, and the treatment duration is prolonged.
5. 3# essential oil cell wall disruption
Alkaline phosphatase (AKP) is present between the cell membrane and the cell wall, and when the bacterial cell wall is damaged, AKP will leak from the cell. Extracellular AKP activity can therefore characterize the integrity and permeability of the bacterial cell wall. Experimental determination of the amount of AKP change in 24h of e.coli and salmonella exposed to different concentrations (control, MIC, MBC) of essential oil 3#, from fig. 5, the phosphatase activity in the control group was very low, which was probably due to normal, low cell death of cells in PBS buffered environment; and a large amount of AKP appears outside escherichia coli and salmonella treated by the 3# essential oil, and by comparing the difference after MIC treatment and MBC treatment, the 3# essential oil can rapidly cause (2 min) cell wall damage, and the effect of the essential oil with high concentration in a certain range is better. The cell wall has the functions of maintaining the normal morphology of cells and controlling the entry and exit of substances, and the damage of the cell wall can ultimately cause cell death.
6. Bacterial death following treatment with # 3 essential oil under fluorescence microscopy
PI is commonly used to confirm the survival status of cells. FIG. 6 is a microscope image (FIG. 6, panel A) and a fluorescence microscope (FIG. 6, panel C-D) of Escherichia coli treated with different concentrations (Control, MIC, MBC) of 3# essential oil, wherein under the fluorescence microscope, it can be seen from FIG. 6 that the cells of the bacteria liquid without essential oil treatment (FIG. 6, panel B) are not stained red, and after the bacteria liquid exposed to the essential oil (FIG. 6, panel C-D) is cultured, the cells are microscopically red, and the area of red fluorescence of the bacteria liquid exposed to MBC under the microscope is obviously larger than that of the bacteria liquid treated with MIC. The results show that complex essential oils can cause bacterial death, and that the higher the concentration of essential oil, the higher the bacterial death rate.
7. The influence of spawning on the female life exists, and whether the high-concentration compound essential oil combination can influence the spawning of the fruit flies is researched on the premise of prolonging the life. The results show that the combination of essential oils has an effect on drosophila oviposition (table 3). There was a significant difference in the amount of eggs laid by female fruit flies in the environment treated with the combination of essential oils compared to the control group. The egg laying amount of the experimental group is obviously reduced.
Table 3: effect of essential oil combination on Drosophila oviposition
8. The 'Y' -shaped tube olfaction measurement result shows that the olfaction reaction of the 3# essential oil combination with different concentrations on female fruit flies is remarkably different from that of a control, which shows that the 3# essential oil combination with different concentrations has remarkable repellent activity on the female fruit flies. And the avoidance effect on the female insects is improved along with the increase of the combined concentration of the 3# essential oil.
9. (1) The waxberries in the control group start to rot, have dark colors, are partially softened, run out of water, are partially cracked, slightly mildew and the like at 5 days, the good fruit rate is 75 percent, and the fruit fly maggots are about 90/kg waxberries; the compound essential oil treatment group is full in color and free of the phenomenon, the fruit yield of the 3# compound essential oil treatment group is up to 98%, and the fruit fly maggots are about 15/kg waxberries. After 10 days, the control group has severe softening, cracking and rotting phenomena, the fruit yield is only 20%, and the fruit flies, maggots and red bayberries are about 520 per kg; in the essential oil treatment group, a small amount of slight mildew appears in the 1% (volume fraction) 3# compound essential oil solution treatment group, the fruit yield is as high as 90%, and the fruit fly maggots are about 75/kg waxberries. The result shows that the 3# compound essential oil can effectively maintain the fruit quality in the preservation of the waxberries.
(2) During picking, the waxberries of the control group and the experimental group are basically in grade 0, and a small amount of waxberries in the control group are in grade 1 and grade 2; after 5 days of picking, the rotted area of the red bayberries of the control group is more than 50% (grade 3) and reaches 34%, and the fruit fly maggots are about 210/kg red bayberries; the treatment group of the 3# compound essential oil solution is only 3 percent, and the number of fruit fly maggots is about 30/kg waxberries. The 3# compound essential oil is sprayed in the growth process of the waxberries, so that the spoilage rate and the insect yield of the waxberries can be effectively reduced, and the quality of the waxberries is improved.
To summarize: now, the trend is more and more towards using compound essential oil, the compound essential oil refers to an essential oil combination mixed according to a certain proportion, and if the essential oil is selected to be proper and the proportion is correct, a synergistic effect can be generated (the synergistic effect refers to the effect of various common efforts which can be found in the decision of resource allocation, namely the effect of ' 1+1> ' 2 '), so that the functions are better realized. The combination of the litsea cubeba essential oil and the orange essential oil improves the antibacterial and insect-resistant performance, and when the litsea cubeba essential oil comprises the following components: when the borneol camphor essential oil = 3.
The above examples are only intended to illustrate the technical solution of the present invention, and not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments described in the foregoing embodiments, or equivalents may be substituted for some of the features thereof; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions.
Claims (4)
1. An essential oil antiseptic insect repellent for waxberries, which is characterized by comprising the following components in percentage by weight: 1% -30% of plant essential oil, 0.1% -10% of tween 80 and the balance of deionized water; the plant essential oil is litsea cubeba essential oil, orange essential oil and borneol camphor essential oil, and the mass ratio is 3:2:1.
2. the essential oil antiseptic insect repellent for red bayberries of claim 1, wherein the plant essential oil content is 10%.
3. An insect-repelling, antiseptic and fresh-keeping method for waxberries, which is characterized in that the fresh waxberries are fumigated by the essential oil antiseptic insect repellent according to any one of claims 1 to 2, wherein the fumigation treatment time is 2 to 6 hours.
4. An insect-repelling, antiseptic and fresh-keeping method for waxberries is characterized in that 15 days, 10 days and 5 days before the waxberries are picked, and the essential oil antiseptic insect-repelling agent according to any one of claims 1 to 2 is uniformly sprayed on waxberry trees.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010115219.1A CN111418648B (en) | 2020-02-25 | 2020-02-25 | Essential oil antiseptic insect repellent for waxberries |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010115219.1A CN111418648B (en) | 2020-02-25 | 2020-02-25 | Essential oil antiseptic insect repellent for waxberries |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN111418648A CN111418648A (en) | 2020-07-17 |
| CN111418648B true CN111418648B (en) | 2023-03-31 |
Family
ID=71547159
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010115219.1A Active CN111418648B (en) | 2020-02-25 | 2020-02-25 | Essential oil antiseptic insect repellent for waxberries |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111418648B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113367118A (en) * | 2021-05-14 | 2021-09-10 | 中国农业科学院上海兽医研究所(中国动物卫生与流行病学中心上海分中心) | Application of Chinese cinnamon essential oil |
| CN114698690A (en) * | 2022-03-30 | 2022-07-05 | 浙江工商大学 | Compound essential oil bacteriostatic preservative for strawberries and process thereof |
| CN115386423A (en) * | 2022-08-29 | 2022-11-25 | 五邑大学 | Dried orange peel essential oil, preparation method and application thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101647474A (en) * | 2009-09-17 | 2010-02-17 | 中国计量学院 | Plant-derived bacteriostatic extractive and application thereof |
| US20150359230A1 (en) * | 2011-11-10 | 2015-12-17 | James J. Messina | Combination animal repellents |
| CN107006601A (en) * | 2017-04-28 | 2017-08-04 | 王耿文 | A kind of preservation method of red bayberry |
| CN107258895A (en) * | 2017-06-23 | 2017-10-20 | 中南林业科技大学 | A kind of anti-corrosive fresh-keeping composition based on litsea citrate oil |
| CN107307262A (en) * | 2017-08-14 | 2017-11-03 | 遵义医学院 | A kind of calamondin essential oil with bacteriostatic activity and preparation method and application |
| CN110178893A (en) * | 2019-07-08 | 2019-08-30 | 四川绿品源贸易有限公司 | A kind of stifling particle of natural fruit and vegetables fungus-resistant combination |
-
2020
- 2020-02-25 CN CN202010115219.1A patent/CN111418648B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101647474A (en) * | 2009-09-17 | 2010-02-17 | 中国计量学院 | Plant-derived bacteriostatic extractive and application thereof |
| US20150359230A1 (en) * | 2011-11-10 | 2015-12-17 | James J. Messina | Combination animal repellents |
| CN107006601A (en) * | 2017-04-28 | 2017-08-04 | 王耿文 | A kind of preservation method of red bayberry |
| CN107258895A (en) * | 2017-06-23 | 2017-10-20 | 中南林业科技大学 | A kind of anti-corrosive fresh-keeping composition based on litsea citrate oil |
| CN107307262A (en) * | 2017-08-14 | 2017-11-03 | 遵义医学院 | A kind of calamondin essential oil with bacteriostatic activity and preparation method and application |
| CN110178893A (en) * | 2019-07-08 | 2019-08-30 | 四川绿品源贸易有限公司 | A kind of stifling particle of natural fruit and vegetables fungus-resistant combination |
Non-Patent Citations (1)
| Title |
|---|
| 龙脑樟鲜叶挥发油成分及其抗菌活性的研究;丁雄等;《食品工业科技》;20121231;第33卷(第18期);第167-171页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN111418648A (en) | 2020-07-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111418648B (en) | Essential oil antiseptic insect repellent for waxberries | |
| Anthony et al. | Fungal pathogens associated with banana fruit in Sri Lanka, and their treatment with essential oils | |
| Landolt et al. | Discovery and development of chemical attractants used to trap pestiferous social wasps (Hymenoptera: Vespidae) | |
| Matsuura et al. | Antifungal activity of a termite queen pheromone against egg-mimicking termite ball fungi | |
| FR2622086A1 (en) | ||
| Zhao et al. | Effect of calcium chloride in combination with salicylic acid on post-harvest freshness of apples | |
| Bahmani et al. | Environment-friendly short-term protection of palm wood against mould and rot fungi | |
| CN103392793B (en) | Preservation technology for bamboos eaten by pandas | |
| Leaver et al. | Antibiotics as a means of control of bacterial contamination of storage tissue disks | |
| KR101820485B1 (en) | Flying insect attracting composition and manufacturing method of plane trap comprising thereof | |
| Raya-González et al. | Dissuasive effect of an aqueous extract from Enterolobium cyclocarpum (Jacq) Griseb on the drywood termite Incisitermes marginipennis (Isoptera: Kalotermitidae)(Latreille). | |
| CN108552205A (en) | Purposes of the farnesol in preventing smut | |
| Roberts et al. | Factors affecting settlement of abalone (Haliotis iris) larvae on benthic diatom films | |
| Soto-Arias et al. | Interaction of phytophagous insects with Salmonella enterica on plants and enhanced persistence of the pathogen with Macrosteles quadrilineatus infestation or Frankliniella occidentalis feeding | |
| JP4581410B2 (en) | Insecticide oil | |
| Idowu et al. | Extracellular enzyme production by microflora from the gut region of the variegated grasshopper Zonocerus variegatus (Orthoptera: Pyrgomorphidae) | |
| Choi et al. | Biocontrol of moth pests in apple orchards: preliminary field study of application potential for mass trapping | |
| Salosso | The potential of forest honey (Apis spp.) from timor island as antibacterial against pathogenic bacteria in fish culture | |
| CN110583767B (en) | Cryptococcus albidus preservative | |
| CN108887553B (en) | Application of bacillus subtilis as mildew preventive in feed | |
| WO2020180490A1 (en) | Bee gut microbial formulation for use as a probiotic for improved bee health and pathogen resistance | |
| KR102019803B1 (en) | A Method for Cultivating a Vegetable worms Having a Function of a Natural Preserved Agent and a Freshness Keeping Agent and an Extract of a Vegetable Worms Cordycepin from the Same and a Natural Complex Composition | |
| US3052598A (en) | Soil fumigant | |
| Tarr | Antioxidants and prevention of rancidity in certain Pacific coast fish | |
| Bisset | False truffle disease of the edible mushroom |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |