CN108552205A - Purposes of the farnesol in preventing smut - Google Patents
Purposes of the farnesol in preventing smut Download PDFInfo
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- CN108552205A CN108552205A CN201810279382.4A CN201810279382A CN108552205A CN 108552205 A CN108552205 A CN 108552205A CN 201810279382 A CN201810279382 A CN 201810279382A CN 108552205 A CN108552205 A CN 108552205A
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- farnesol
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N49/00—Biocides, pest repellants or attractants, or plant growth regulators, containing compounds containing the group, wherein m+n>=1, both X together may also mean —Y— or a direct carbon-to-carbon bond, and the carbon atoms marked with an asterisk are not part of any ring system other than that which may be formed by the atoms X, the carbon atoms in square brackets being part of any acyclic or cyclic structure, or the group, wherein A means a carbon atom or Y, n>=0, and not more than one of these carbon atoms being a member of the same ring system, e.g. juvenile insect hormones or mimics thereof
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Abstract
The present invention discloses purposes of the farnesol in prevention sugarcane smut and corn smut, it can influence the history of life of sugarcane smut, it is proliferated in growth and sexual cooperation all with preferable inhibition in the sprouting of teleutospore, basidiospore, the formation of dicaryon mycelium can effectively be blocked, cause sugarcane smut that cannot normally infect sugarcane, to effectively inhibit the generation of sugarcane smut；The same proliferation growth for influencing ustilago zeae, inhibits the generation of corn smut.
Description
Technical field
The present invention relates to technical field of biological control, and in particular to application of the farnesol in preventing smut.
Background technology
Smut is mainly by the plant disease caused by smut, according to incompletely statistics, about 1200 kinds of smut,
4000 various plants can be infected, their host includes careless section plant and important crops are such as in the world：It is corn, sugarcane, barley, small
Wheat, oat, sorghum etc..Smut is characterized in forming the teleutospore of black powdery in heaps in the tissue for infecting host.
Corn and sugarcane are main cereal crops in the world, are also important Bio-Energy Material and forage crop.
Corn smut and sugarcane smut (Sporisorium caused by Ustilago maydis (Ustilago maydis)
Scitamineum sugarcane smut caused by) is the Major Diseases for influencing two kinds of crop yields and quality respectively.Currently, prevention
The measure that sugarcane smut and corn smut use includes selection and breeding and promotes disease-resistant variety and chemical prevention, but current prevention
Measure can not effectively preventing disease.
Farnesol (Farnesol) molecular formula is C15H26O is a kind of sesquiterpenoid, 3,7,11- trimethyls -2,6,
10- dodecane triolefin -1- alcohol, isomer are divided into trans-, trans- cis-, cis- trans- or cis-, and structure is as follows：
It is naturally occurring in joyous alloy, Brazil's sandalwood, Semen seu folium abelmoschi moschati, rose fragrant, jasmine and the essence of flores aurantii, is mainly used for
Fragrance or the food flavor for allocating fruit.Also there is juvenile hormone activity, can inhibit metamorphosis and the maturation of insect, make larva not
Energy pupa, pupa cannot become adult, and adult does not lay eggs, so it is also a kind of insecticide.Its low toxicity, safety are naturally occurring in
Can also very big application prospect be had by soil microbial degradation in environment.
Farnesol (Farnesol) is also a kind of mycetogenetic small-molecule substance (QSM), can adjust cellular morphology, life
Other processes of length, biofilm formation, anti-oxidation stress and thalline life.Correlative study finds that farnesol (Farnesol) can be with
The mycelia of Candida albicans is formed, cell wall maintains, iron transfer and the related genes such as heat shock protein and drug resistance have
It is apparent to influence.According to Machida, the farnesol of 25 μm of ol/L can make saccharomyces cerevisiae G1 cell cycle arrests and intracellular
Diacylglycerol (DAG) is horizontal to be significantly reduced, to inhibit growth without damaging cell viability；Lorek etc. has found farnesol
Have an impact to the form of aspergillus niger, can completely inhibit conidium higher than 10mmol/L in concentration grows and lead to cyclic adenosine monophosphate
(cAMP) decline of 10 times of level；Dichtl etc. is research shows that farnesol inhibits human pathogen aspergillus fumigatus (Aspergillus
Fumigatus) wild-type cell is grown, and as cell wall stress agent, is inhibited cell wall integrity signal path, is caused
Tiplocalized Rho albumen locations of mistake cause hypha form problem；Farnesol is to Fusarium graminearum (Candida
Dubliniensis) have the function of inducing cell apoptosis and influence macroconidium generation, development, sprout, survival, in addition method
Buddhist nun's alcohol can inhibit mycobacterium smegmatis (Mycobacterium smegmatis) drug efflux pump, reduce ethidium bromide and profit
The flat minimum inhibitory concentration to mycobacterium smegmatis of good fortune.Therefore farnesol can be used as potential new type bactericide.
Invention content
The new application that it is an object of the invention to invention farnesols in preventing smut.
Technical solution provided by the invention is：Farnesol to the sprouting of sugarcane smut and ustilago zeae, growth and
Application in the inhibition of sexual cooperation.
The farnesol has extensive production source, can be from being extracted in extraction, microorganism in the plants such as Siraitia grosvenorii
Or artificial chemistry synthesis.The farnesol is dissolved in after ethyl alcohol, hydrone dispersant is made, then handled respectively in the black powder of sugarcane
On germ and ustilago zeae, its fungistatic effect is observed.
In the present invention, the minimum effective concentration that farnesol inhibits sugarcane brand spores to sprout is 1.0 μm；Farnesol presses down
The minimum effective concentration of sugarcane smut growth processed is 0.325 μm；Farnesol effectively inhibits the sexual cooperation mycelia of sugarcane smut
The minimum concentration of growth is 0.04 μm.
Based on later production application cost control, the present invention provides the farnesol using a concentration of 0.01~5 μm application
In prevention sugarcane smut and corn smut.
Compared with prior art, the present invention has the advantages that：
1. the best of itself for the first time using mycetogenetic signal small molecule farnesol as fungicide, is explored in experiment to be made
And, theoretical foundation is provided for exploitation prevention sugarcane smut medicament, the green to realize Sugarcane Industry is sustainable with concentration
Development is contributed share for the booming of national economy.Simultaneously also responsive to the Government idea for subtracting pesticide grown, reach pesticide zero growth rate
Target, walk environmentally friendly conservation culture road.
2. farnesol (Farnesol) is used as a kind of mycetogenetic signal small molecule, it can influence mycelia and be formed, is raw
Object film is formed and some response to oxidative stress, regulating cell form, in fungal attack important role.This experiment for the first time will
Farnesol is studied as a kind of fungicide to prevent sugarcane smut, and compared with traditional fungicide, farnesol is used as and kills
There is microbial inoculum green, safety to be naturally occurring in environment can also be by soil microbial degradation the advantages that.Its application study is also located
In the starting stage, research has novelty.
On the whole, the present invention develops farnesol at a kind of new and effective sterilizing synergistic agent in controlling plant diseases
With the bright outlook.
Description of the drawings
Fig. 1 is inhibition figure of the farnesol to sugarcane brand spores.Wherein：Arrow meaning is not sprouted in figure
Spore.
Fig. 2 is the virulence design sketch that farnesol is proliferated two kinds of basidiospore of sugarcane smut.Wherein：Fig. 2A is farnesol pair
WT17 is proliferated toxicity action, and Fig. 2 B farnesols are to being that WT18 is proliferated toxicity action.
Fig. 3 is toxicity action bacterium colony figure of the farnesol to sugarcane smut (WT17), wherein：3A:CK；3B:0.4μM
Farnesol；3C:0.6μM Farnesol；3D:0.8μM Farnesol；3E:1.0μM Farnesol.
Fig. 4 is toxicity action bacterium colony figure of the farnesol to sugarcane smut (WT18), wherein：4A:CK；4B:0.4μM
Farnesol；4C:0.6μM Farnesol；4D:0.8μM Farnesol；4E:1.0μM Farnesol.
Fig. 5 is toxicity action bacterium colony figure of the farnesol to ustilago zeae (U9), wherein：5A:CK；5B:Ethyl alcohol；5C:0.4
μM Farnesol；5D:1μM Farnesol.
Fig. 6 is the inhibiting effect figure that farnesol grows sugarcane smut mycelia, wherein：6A:MM；6B:MM+1%
Ethanol；6C:MM+0.1μM Farnesol；6D:MM+0.5μM Farnesol.
Fig. 7 is function and effect figure of the various concentration farnesol to the sexual cooperation of sugarcane smut, wherein：Eth is that ethyl alcohol is molten
Agent compares.
Fig. 8 is farnesol to sugarcane smut (WT17) single bacterium colony growth inhibition figure, wherein：8A:CK；7B:1%
Ethanol；8C:0.1μM Farnesol；8D:0.5μM Farnesol.
Fig. 9 is farnesol to sugarcane smut (WT18) single bacterium colony growth inhibition figure, wherein：9A:CK；9B:1%
Ethanol；9C:0.1μM Farnesol；9D:0.5μM Farnesol.
Specific implementation mode
Below by specific implementation mode detailed description come the present invention is furture elucidated, but be not to the present invention limit
System, only illustrates.
1.1 material to be tested
Farnesol buys gained, CAS Number from sigma companies:4602-84-0.And by the anhydrous second of the farnesol
It is spare that alcohol is diluted to a concentration of 3.98mm mother liquors.The sugarcane of Guangxi field natural occurrence is picked up from for examination sugarcane smut bacterium teleutospore
Smut plant, a pair of of sugarcane smut bacterium basidiospore WT17, WT18 for examination are teleutospore point on the sugarcane smut diseased plant
From gained, and it is both identified be respectively "+" and-" monoploid, can be mutually distinguishable, carry out sexual cooperation generation amphiploid bacterium
Silk, and U9, U10 be ustilago zeae be laboratory preservation.Culture solution used in this laboratory is YEPSA+0.05% glycerine.
1.2 for trying culture medium
YEPSA culture mediums：Yeast extract 10g, peptone 20g, sucrose 20g, agar 18g, add deionized water to be settled to
Conventional sterilant after 1000m L.YEPS fluid nutrient mediums：It is not added with the YEPSA culture mediums of agar.Culture medium adds when in use
0.5% glycerine hydrotropy.
Embodiment 1
On the basis of pilot study, first by farnesol with YEPS fluid nutrient mediums be configured to a certain concentration gradient (0 μM,
2.5 μM, 5 μM, 10 μM) mother liquor it is spare.Teleutospore is scraped from acquisition fresh cane smut standard specimen again, is trained with YEPS liquid
Teleutospore suspension (5 × 10 is made in foster base4A/ml).200 μ l to the 2ml sterile centrifugation tubes of mother liquor of various concentration are drawn respectively
In, the teleutospore suspension mixing of equivalent volumes is added, as a contrast with the YEPS of not adding medicine.Each drug concentration is arranged three
It repeats, each processing is put in 28 DEG C, after cultivating 6h in 200rpm/min shaking tables, each teliospore germination situation of microscopy records 100
The quantity sprouted in teleutospore calculates inhibiting rate.
Experimental result (referring to Fig. 1), after cultivating 6h in shaking table, the germination rate of control group reaches 85% or more, additive process
Germination rate is declined in the processing of Buddhist nun's alcohol, and germination rate is when as its a concentration of 0.5um, germination rate is 51%, 1.0um
7.9%.
Embodiment 2
On the basis of pilot study, first by farnesol with YEPS fluid nutrient mediums be configured to a certain concentration gradient (0 μM,
0.5 μM, 0.8 μM, 1 μM, 2 μM, 4 μM, 6 μM, 8 μM, 9 μM, 10 μM, 20 μM) mother liquor it is spare.The a little sugarcane of picking from tablet
Smut basidiospore is incubated overnight in 6mlYEPS culture solutions as seed liquor, and next day, thalline were collected by centrifugation, is trained with YEPS liquid
It supports base weight and hangs thalline (OD600It is 0.2) for use.It draws respectively in 200 μ l various concentrations mother liquors to 2ml sterile centrifugation tubes, be added etc.
The basidiospore suspension mixing of amount is control with not adding medicine YEPS fluid nutrient mediums, each concentration processing three repetitions of setting.So
Starting OD is respectively handled with spectrophotometer measurement afterwards600Afterwards, 28 DEG C are placed in, 20h is cultivated in 200rpm/min shaking tables, it is each measuring
The final OD of processing600, by calculating OD600Net increment finds out inhibiting rate, virulence regression equation, related coefficient and EC50。
Experimental result is referring to Fig. 2A, 2B, table 1.Wherein, table 1 is the virulence level statistics to two kinds of basidiospore.Experimental result
Display farnesol has toxicity action to two kinds of basidiospore of sugarcane smut, can inhibit its proliferation growth.And inhibiting effect
It can weaken with the reduction of concentration, WT18 type basidiospore is more sensitive when in face of high concentration farnesol, hardly grows.Method
Buddhist nun's alcohol has obvious inhibiting effect, virulence equation to be shown in Table 1 two kinds of basidiospore of sugarcane smut.
Table 1：The virulence level that farnesol is proliferated sugarcane smut bacterium basidiospore
Embodiment 3
On the basis of pilot study, first by farnesol with YEPS fluid nutrient mediums be configured to a certain concentration gradient (0 μM, 8
μM, 12 μM, 16 μM, 20 μM) mother liquor it is spare.From a little sugarcane smut of picking and ustilago zeae carry on a shoulder pole spore from tablet respectively
Son is incubated overnight in 6mlYEPS culture solutions as seed liquor, and next day, which transfers, expands culture in 500 μ l to 50mlYEPS culture solutions
(to OD6000.2), in 500 μ l to the 5ml sterile centrifugation tubes of mother liquor for drawing various concentration respectively, it is mixed that isometric bacterium solution to be added
It is even, 28 DEG C are placed in, 12h is cultivated in 200rpm/min shaking tables.The bacterium for then being handled each concentration with fresh YEPS fluid nutrient mediums
Liquid dilution 104Times, it draws 200 μ l and is coated on YEPSA solid mediums, be placed in stationary culture 48h in 28 DEG C of incubators.Finally
Fungistatic effect is observed, colony counts are counted.
Experimental result (referring to Fig. 3, Fig. 4, Fig. 5), by count colony counts obtain, sugarcane smut bacterium basidiospore containing
It is grown on the tablet of Farnesol, when concentration reaches 0.4 μM, colony counts reduce 50%, and 98% or more is reduced at 1 μM；Corn
Smut basidiospore reduces 90% or more in 0.4 μM of colony counts, and bactericidal effect is more notable.
Embodiment 4
Farnesol is dissolved in by various concentration in MMA solid mediums, pours into a diameter of 9cm culture dishes that drug containing is made is flat
Plate.By WT17 and WT18 from scraped on tablet a little in 6mlYEPS fluid nutrient mediums 28 DEG C, 200rpm/min culture make for 24 hours
For seed liquor, thalline is then collected.It is OD with the resuspension of MM fluid nutrient mediums600After 1, two kinds of isometric mixings of basidiospore, respectively
It takes 1.5 μ l bacterium suspensions points on the tablet containing farnesol of various concentration (0 μM, 0.1 μM, 0.5 μM, 1 μM), waits for that surface moisture is inhaled
After dry, it is placed in stationary culture in 28 DEG C of incubators, each concentration processing three repetitions of setting.It is placed in 28 DEG C of incubators and stands training
It supports for 24 hours, with stereoscopic sem observation mycelia growing state, inhibition is referring to Fig. 6.The experimental results showed that smut mycelia grows
It is very sensitive to farnesol, the just completely not long mycelia under 0.1 μM of drug containing tablet, and under the influence of 0.5 μM of farnesol, life
Length is all heavily suppressed, and bacterium colony growth becomes sparse, slowly.
Embodiment 5
Farnesol is dissolved in absolute ethyl alcohol, configuration various concentration gradient (0 μM, 1 μM, 2 μM, 4 μM, 6 μM, 8 μM, 10 μM) is molten
Liquid is as test fluid.Farnesol fungistatic effect is measured using 24 hole polystyrene plates, 1mlYEPEA and 10 μ is added in each hole
3 repetitions are arranged in l test fluids, each concentration, and the culture medium of not dosing makees blank control.WT17 and WT18 are scraped from tablet few
Perhaps it is used as seed liquor for 24 hours in 28 DEG C, 200rpm/min cultures in 6mlYEPS fluid nutrient mediums, then collects thalline.With MM liquid
It is OD that body culture medium, which is resuspended,600After 1, two kinds of isometric mixings of basidiospore take 1.5 μ l bacterium suspension points in various concentration respectively
Tablet containing farnesol on, after surface moisture blots, be placed in stationary culture in 28 DEG C of incubators, each concentration processing setting three
A repetition.It is as follows to be placed in the stereoscopic sem observation mycelia growing states of stationary culture 48h, inhibition figure in 28 DEG C of incubators.
Experimental result (referring to Fig. 7), the results show that being reduced with farnesol concentration, sugarcane smut both sexes ligand in figure
It can gradually form white hypha, and mycelia will be inhibited to be formed when 0.01 μM of farnesol, and 0.04 μM of when, inhibits mycelia to be formed
Effect become apparent from.
Embodiment 6
Farnesol is dissolved in by various concentration (0 μM, 0.1 μM, 0.5 μM) in YEPSA solid mediums, is poured into a diameter of
Drug containing tablet is made in 9cm culture dishes.By WT17 and WT18 from being scraped on tablet a little in 6mlYEPS fluid nutrient mediums 28
DEG C, 200rpm/min culture for 24 hours be used as seed liquor, then collect thalline.It is OD with the resuspension of MM fluid nutrient mediums600After 2, point
It does not take 1.5 μ l bacterium suspension points on the tablet containing farnesol of various concentration, after surface moisture blots, is placed in 28 DEG C of incubators
Middle stationary culture, each concentration processing three repetitions of setting.Stationary culture is placed in 28 DEG C of incubators for 24 hours, with stereoscopic sem observation
Thalli growth situation.Its inhibition is referring to Fig. 8, Fig. 9.By finding, compared to blank control, to add the culture of 1% ethyl alcohol in figure
Two kinds of basidiospore of base pair have minimal effect, and its effect is notable after adding farnesol, causes most of spore death that can not grow,
And two kinds of basidiospore growths of farnesol pair all have toxicity action.
Claims (8)
1. application of the farnesol in the inhibition to the sprouting of sugarcane smut or ustilago zeae, growth and sexual cooperation.
2. application according to claim 1, it is characterised in that：The farnesol molecular formula is C15H26O, 3,7,11- front threes
Base -2,6,10- dodecane triolefin -1- alcohol.
3. application according to claim 1, it is characterised in that：The isomer of the farnesol is trans-, trans-
It is cis-, cis- trans- or cis-.
4. application according to claim 2, which is characterized in that farnesol is to be extracted from plant.
5. application according to claim 1, which is characterized in that it is minimum that farnesol inhibits sugarcane brand spores to sprout
Effective concentration is 1.0 μm.
6. application according to claim 1, which is characterized in that farnesol inhibits the minimum effectively dense of sugarcane smut growth
Degree is 0.325 μm.
7. application according to claim 1, which is characterized in that farnesol effectively inhibits the sexual cooperation mycelia of sugarcane smut
The minimum concentration of growth is 0.04 μm.
8. application of the farnesol in prevention sugarcane smut or corn smut, which is characterized in that the farnesol is using dense
Ranging from 0.01 μm ~ 5 μm of degree.
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| CN201810279382.4A CN108552205B (en) | 2018-04-01 | 2018-04-01 | Application of farnesol in preventing and treating powdery mildew |
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| CN201810279382.4A CN108552205B (en) | 2018-04-01 | 2018-04-01 | Application of farnesol in preventing and treating powdery mildew |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113841707A (en) * | 2021-10-26 | 2021-12-28 | 中国热带农业科学院环境与植物保护研究所 | Application of trans-farnesol as synergist in preventing and treating litchi downy blight |
| CN114391551A (en) * | 2022-02-10 | 2022-04-26 | 黄业群 | Novel special fertilizer for strawberries |
| CN116267925A (en) * | 2023-03-03 | 2023-06-23 | 贵州大学 | Application of farnesol as agricultural bactericide in plant bacterial diseases |
| CN114946873B (en) * | 2020-11-04 | 2023-11-21 | 江苏省农业科学院 | Application of farnesol in preventing and treating false smut |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114946873B (en) * | 2020-11-04 | 2023-11-21 | 江苏省农业科学院 | Application of farnesol in preventing and treating false smut |
| CN113841707A (en) * | 2021-10-26 | 2021-12-28 | 中国热带农业科学院环境与植物保护研究所 | Application of trans-farnesol as synergist in preventing and treating litchi downy blight |
| CN113841707B (en) * | 2021-10-26 | 2022-07-05 | 中国热带农业科学院环境与植物保护研究所 | Application of trans-farnesol as synergist in preventing and treating litchi downy blight |
| CN114391551A (en) * | 2022-02-10 | 2022-04-26 | 黄业群 | Novel special fertilizer for strawberries |
| CN114391551B (en) * | 2022-02-10 | 2023-09-19 | 艳阳生物科技(山东)有限公司 | Novel fertilizer special for strawberries |
| CN116267925A (en) * | 2023-03-03 | 2023-06-23 | 贵州大学 | Application of farnesol as agricultural bactericide in plant bacterial diseases |
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| CN108552205B (en) | 2020-12-11 |
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