CN111417715B - 细胞培养方法 - Google Patents

细胞培养方法 Download PDF

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Publication number
CN111417715B
CN111417715B CN201880076960.2A CN201880076960A CN111417715B CN 111417715 B CN111417715 B CN 111417715B CN 201880076960 A CN201880076960 A CN 201880076960A CN 111417715 B CN111417715 B CN 111417715B
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cysteine
cystine
cell culture
seq
group
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Chinese (zh)
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CN111417715A (zh
Inventor
V·车瓦里尔
N·寇查诺夫斯基
L·马菲特斯
V·A·C·库尔
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UCB Biopharma SRL
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UCB Biopharma SRL
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/46—Hybrid immunoglobulins
    • C07K16/461—Igs containing Ig-regions, -domains or -residues form different species
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/515—Complete light chain, i.e. VL + CL
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/565—Complementarity determining region [CDR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
CN201880076960.2A 2017-12-01 2018-11-29 细胞培养方法 Active CN111417715B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP17204978.5A EP3492582A1 (en) 2017-12-01 2017-12-01 Cell culture methods
EP17204978.5 2017-12-01
PCT/EP2018/083010 WO2019106091A1 (en) 2017-12-01 2018-11-29 Cell culture methods

Publications (2)

Publication Number Publication Date
CN111417715A CN111417715A (zh) 2020-07-14
CN111417715B true CN111417715B (zh) 2024-08-27

Family

ID=60629439

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201880076960.2A Active CN111417715B (zh) 2017-12-01 2018-11-29 细胞培养方法

Country Status (14)

Country Link
US (1) US11859203B2 (https=)
EP (2) EP3492582A1 (https=)
JP (1) JP7422072B2 (https=)
KR (1) KR20200092348A (https=)
CN (1) CN111417715B (https=)
BR (1) BR112020010473A2 (https=)
CA (1) CA3083680A1 (https=)
EA (1) EA202091349A1 (https=)
ES (1) ES2963653T3 (https=)
HR (1) HRP20231175T1 (https=)
HU (1) HUE063812T2 (https=)
PL (1) PL3717631T3 (https=)
SG (1) SG11202004935UA (https=)
WO (1) WO2019106091A1 (https=)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3492582A1 (en) 2017-12-01 2019-06-05 UCB Biopharma SPRL Cell culture methods
CN112391350A (zh) * 2020-10-30 2021-02-23 北京双鹭药业股份有限公司 高效表达抗egfr单克隆抗体细胞的筛选培养方法
GB202105424D0 (en) 2021-04-16 2021-06-02 UCB Biopharma SRL Cell culture processes
EP4166651A1 (en) 2021-10-18 2023-04-19 Evonik Operations GmbH Methods and composition to increase cellular glutathione level
WO2023067626A1 (en) * 2021-10-20 2023-04-27 Dr. Reddy's Laboratories Limited A cell culture process to produce an antibody composition
EP4484567A4 (en) * 2022-02-21 2026-04-01 Chugai Pharmaceutical Co Ltd PERFUSION CULTURE METHOD
WO2024024720A1 (ja) * 2022-07-25 2024-02-01 味の素株式会社 細胞培養用の培地

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017186654A1 (en) * 2016-04-28 2017-11-02 Merck Patent Gmbh Method for reducing the trisulfide level in proteins

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Publication number Priority date Publication date Assignee Title
US4927762A (en) 1986-04-01 1990-05-22 Cell Enterprises, Inc. Cell culture medium with antioxidant
EP1482031B1 (en) 1996-08-30 2015-10-28 Life Technologies Corporation Serum-free mammalian cell culture medium, and uses thereof
CN101332157B (zh) * 1998-10-09 2012-01-11 味之素株式会社 半胱氨酸衍生物
KR20050070512A (ko) 2003-12-30 2005-07-07 삼성정밀화학 주식회사 재조합 단백질의 생산성을 증가시키는 무혈청 배지 및동물세포 배양방법
TWI456062B (zh) * 2006-09-13 2014-10-11 Abbvie Inc 細胞培養改良
EP2500415A1 (en) 2006-09-13 2012-09-19 Abbott Laboratories Cell culture improvements
AU2011246502B2 (en) 2010-04-26 2014-08-28 Novartis Ag Improved cell cultivation process
WO2013158275A1 (en) 2012-04-20 2013-10-24 Abbvie Inc. Cell culture methods to reduce acidic species
US20130281355A1 (en) 2012-04-24 2013-10-24 Genentech, Inc. Cell culture compositions and methods for polypeptide production
GB201208367D0 (en) 2012-05-14 2012-06-27 Ucb Pharma Sa Biological product
BR112016027797A2 (pt) 2014-06-18 2017-10-31 Medimmune Llc métodos e meios de cultura de células compreendendo n-acetilcisteína
EP3492582A1 (en) 2017-12-01 2019-06-05 UCB Biopharma SPRL Cell culture methods

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017186654A1 (en) * 2016-04-28 2017-11-02 Merck Patent Gmbh Method for reducing the trisulfide level in proteins

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Intracellular redox regulation by a cystine derivative suppresses UV-induced NF-UB activation;Manabu Kitazawa等;FEBS Letters;第526卷;第106页右栏第3段 *

Also Published As

Publication number Publication date
PL3717631T3 (pl) 2024-03-04
JP7422072B2 (ja) 2024-01-25
EA202091349A1 (ru) 2020-08-20
US20200308536A1 (en) 2020-10-01
SG11202004935UA (en) 2020-06-29
EP3717631A1 (en) 2020-10-07
US11859203B2 (en) 2024-01-02
CA3083680A1 (en) 2019-06-06
JP2021503938A (ja) 2021-02-15
HRP20231175T1 (hr) 2024-01-19
BR112020010473A2 (pt) 2020-11-24
KR20200092348A (ko) 2020-08-03
WO2019106091A1 (en) 2019-06-06
EP3717631B1 (en) 2023-08-30
CN111417715A (zh) 2020-07-14
HUE063812T2 (hu) 2024-02-28
EP3717631C0 (en) 2023-08-30
ES2963653T3 (es) 2024-04-01
EP3492582A1 (en) 2019-06-05

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