CN111413307A - Up-conversion fluorescence detection device and detection method - Google Patents
Up-conversion fluorescence detection device and detection method Download PDFInfo
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- CN111413307A CN111413307A CN202010276226.XA CN202010276226A CN111413307A CN 111413307 A CN111413307 A CN 111413307A CN 202010276226 A CN202010276226 A CN 202010276226A CN 111413307 A CN111413307 A CN 111413307A
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- 238000006243 chemical reaction Methods 0.000 title claims abstract description 43
- 238000001917 fluorescence detection Methods 0.000 title claims abstract description 25
- 238000001514 detection method Methods 0.000 title abstract description 29
- 230000005284 excitation Effects 0.000 claims abstract description 31
- 238000012937 correction Methods 0.000 claims abstract description 9
- 230000005540 biological transmission Effects 0.000 claims description 19
- 238000000034 method Methods 0.000 claims description 8
- 239000011324 bead Substances 0.000 claims description 6
- 239000003068 molecular probe Substances 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 5
- 238000003825 pressing Methods 0.000 claims description 4
- 230000000149 penetrating effect Effects 0.000 claims description 3
- 239000003381 stabilizer Substances 0.000 claims description 3
- 238000004458 analytical method Methods 0.000 description 4
- 238000009434 installation Methods 0.000 description 4
- 239000000969 carrier Substances 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 238000009792 diffusion process Methods 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 238000009736 wetting Methods 0.000 description 2
- 238000007792 addition Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010835 comparative analysis Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000004020 luminiscence type Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N2021/0106—General arrangement of respective parts
- G01N2021/0112—Apparatus in one mechanical, optical or electronic block
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention discloses an up-conversion fluorescence detection device and a detection method, wherein the detection device comprises a conveying device, a power device for driving the conveying device, an excitation device and a camera device which are arranged on the side edge of the conveying device, and a correction device and a leveling device which are arranged at one end of the conveying device, wherein the correction device is used for assisting a worker to correct the position of a carrier on the conveying device and ensuring the accuracy of subsequent detection.
Description
Technical Field
The invention belongs to the technical field of fluorescence detection, and particularly relates to an up-conversion fluorescence detection device and a detection method.
Background
The upper conversion luminescence is derived from Stokes' law, which considers that the material is intelligently excited by high-energy light and emits low-energy light, and the application of the upper conversion technology to the actual detection is to use the converted fluorescent material as a raw material to manufacture corresponding detection test paper and detect according to the concentration of a reactant generated by a test target and the test paper;
the up-conversion fluorescence detection device in the prior art mainly adopts a rotating disc type detection device, particularly, carriers are fixed on a rotating disc, and then the carriers on the rotating disc are subjected to optical excitation one by one.
Disclosure of Invention
The invention aims to provide an up-conversion fluorescence detection device and a detection method.
The technical problems to be solved by the invention are as follows:
the up-conversion fluorescence detection device in the prior art mainly adopts a rotating disc type detection device, particularly fixes a carrier on a rotating disc, and then carries out optical excitation on the carrier on the rotating disc one by one, the detection of a plurality of carriers can be completed by the method at one time, but because the detection time is not uniform, the consistency influence of the detection result is large, and the carrier positions distributed on the rotating disc in an annular array need to be kept consistent, so that the requirement on the setting position of the carrier is high, and the convenience and the accuracy of analysis and detection are ensured.
The purpose of the invention can be realized by the following technical scheme:
an up-conversion fluorescence detection device comprises a conveying device, wherein the conveying device is driven by a power device, an excitation device and a camera device are arranged on the side edge of the conveying device, and a correction device and a leveling device are arranged at one end of the conveying device;
the conveying device comprises a base station, a light transmission groove is formed in the middle of the base station, a camera light transmission groove is formed in the middle of the base station, roll shafts are rotatably arranged at two ends of the base station, two ends of each roll shaft are rotatably connected with the base station through bearings, and the two roll shafts are connected through a conveying belt;
the excitation device comprises a sliding main rod, a convex sliding rail is arranged on one side of the sliding main rod, the sliding main rod is connected with two sliding blocks in a sliding mode through the sliding rail, one sliding block is fixedly connected with a light source fixing plate, the other sliding block is fixedly connected with a light transmitting ring fixing plate, an excitation light source is fixedly arranged on the light source fixing plate, and a circular light transmitting ring penetrating through the light transmitting ring fixing plate is arranged on the light transmitting ring fixing plate; the light-transmitting ring is arranged right below the light-emitting point of the excitation light source.
As a further scheme of the invention, the power device comprises a driving motor, a shaft extension end of the driving motor is connected with a transmission shaft, a motor shell of the driving motor is fixedly connected with an installation frame through a plurality of stabilizer bars, the installation frame is fixedly installed on a rack, a gear is fixedly sleeved on the transmission shaft, gears are respectively sleeved at two ends of a roller shaft, and the gear on the roller shaft is connected with the gear sleeved on the transmission shaft through a belt or a chain.
As a further scheme of the invention, the correction device comprises a main rod, wherein two ends of the main rod are rotatably connected with threaded rods, threaded sleeves are sleeved on the threaded rods and are in threaded fit with the threaded rods, the threaded sleeves are further sleeved on the main rod and are in sliding fit with the main rod, mounting rods are further fixedly mounted on the threaded sleeves, mounting plates are fixedly mounted on the mounting rods, laser lamp beads are fixedly mounted on the mounting plates, and laser rays of the laser lamp beads are projected to one point of the surface of the conveyor belt.
As a further scheme of the invention, the leveling device comprises a leveling main rod, an adjusting mounting plate is fixedly mounted at the end part of the leveling main rod through a bolt, a strip-shaped hole is formed in the adjusting mounting plate, a mounting block is fixedly mounted on the adjusting mounting plate through a bolt, and the mounting block is rotatably connected with one end of the pressing shaft.
As a further aspect of the present invention, the excitation light source is a multi-spectral L ED light source.
As a further aspect of the present invention, the image pickup device is an industrial camera.
The method for detecting the up-conversion fluorescence by the up-conversion fluorescence detection device comprises the following steps:
s1, reacting the up-conversion fluorescent molecular probe with an object to be detected on a carrier, wherein the up-conversion fluorescent molecular probe is a material which is excited by high-energy light and emits low-energy light;
and S2, fixing the reacted carrier on a conveyor belt, driving the conveyor belt to rotate by a power device, transmitting the carrier to the lower part of an excitation light source, starting the excitation light source to irradiate the carrier for a preset time T, transmitting the carrier to the vicinity of the camera device, and shooting by the camera device to obtain a strip band generated by the fluorescence reaction on the corresponding carrier.
The invention has the beneficial effects that:
the invention relates to an up-conversion fluorescence detection device, which comprises a conveying device, a power device for driving the conveying device, an excitation device and a camera device which are arranged on the side edge of the conveying device, and a correction device and a leveling device which are arranged at one end of the conveying device, wherein the conveying device and the power device are used for transferring a carrier to be detected so that the carrier can be continuously and sequentially detected, the correction device is used for assisting a worker to correct the position of the carrier on the conveying device, and a strip generated by fluorescence reaction on the carrier is positioned at a preset position in a picture in a subsequent detection process, so that the accuracy of a detection result is ensured, the carrier passing through the leveling device can be flattened by the aid of the fact that the paper carrier is easy to deform in the conveying and wetting and drying processes, so that the strip generated by the fluorescence reaction on the carrier collected by the camera device is deformed in the subsequent detection process, so that the contrast analysis is difficult, the accuracy of the detection result is influenced, the excitation device comprises a light transmission ring fixing plate and an excitation light source L serving as a lamp bead, the light transmission ring is arranged right below a light spot, the light transmission ring is arranged, the diffusion reaction of the excitation light source is limited, the fluorescent light source detection device is capable of reducing the accuracy, and the fluorescent light transmission detection accuracy of the fluorescent light source detection device, and the fluorescent light detection is reduced.
Drawings
The invention is described in further detail below with reference to the figures and specific embodiments.
FIG. 1 is a schematic structural diagram of an up-conversion fluorescence detection device according to the present invention;
FIG. 2 is a schematic diagram of the excitation device;
FIG. 3 is a schematic view of the orthotic device;
FIG. 4 is a schematic structural view of a transfer device;
FIG. 5 is a schematic structural diagram of a power plant;
figure 6 is a schematic view of the structure of the flattening apparatus.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
An up-conversion fluorescence detection device, as shown in fig. 1 to 6, comprises a conveying device 1, wherein the conveying device 1 is driven by a power device 2, an excitation device 3 and an image pickup device 4 are arranged at the side edge of the conveying device 1, and a correction device 5 and a leveling device 6 are arranged at one end of the conveying device 1;
the conveying device 1 comprises a base platform 11, a light transmission groove 12 is arranged in the middle of the base platform 11, a camera light transmission groove 13 is arranged in the middle of the base platform 11, roller shafts 14 are rotatably arranged at two ends of the base platform 11, two ends of each roller shaft 14 are rotatably connected with the base platform 11 through bearings, and the two roller shafts 14 are connected through a conveying belt;
the power device 2 comprises a driving motor 21, a shaft extension end of the driving motor 21 is connected with a transmission shaft, a motor shell of the driving motor 21 is fixedly connected with an installation frame 23 through a plurality of stabilizer bars 22, the installation frame 23 is fixedly installed on a rack, gears are fixedly sleeved on the transmission shaft, gears are respectively sleeved at two ends of the roll shaft 14, and the gears on the roll shaft 14 are connected with the gears sleeved on the transmission shaft through belts or chains;
the correcting device 5 comprises a main rod 51, wherein two ends of the main rod 51 are rotatably connected with a threaded rod 52, a threaded sleeve 53 is sleeved on the threaded rod 52, the threaded sleeve 53 is in threaded fit with the threaded rod 52, the threaded sleeve 53 is further sleeved on the main rod 51, the threaded sleeve 53 is in sliding fit with the main rod 51, a mounting rod 54 is further fixedly mounted on the threaded sleeve 53, a mounting plate 55 is fixedly mounted on the mounting rod 54, a laser lamp bead 56 is fixedly mounted on the mounting plate 55, a laser ray is emitted through the correcting device 5, the laser ray is projected to a certain point of a conveyor belt, the position of the carrier is adjusted through the position of the laser spot, the correcting device is used for assisting a worker to correct the position of the carrier on the conveying device, and the strip generated by fluorescence reaction on the carrier is in the preset position in a photo in the subsequent detection process, so that the accuracy of a detection result is,
the leveling device 6 comprises a leveling main rod 61, an adjusting mounting plate 62 is fixedly mounted at the end part of the leveling main rod 61 through a bolt, a strip-shaped hole is formed in the adjusting mounting plate 62, a mounting block 63 is fixedly mounted on the adjusting mounting plate 62 through a bolt, the mounting block 63 is rotatably connected with one end of a pressing shaft 64, the pressing shaft 64 is used for extruding and leveling the carrier on the conveying belt, and the paper carrier is easy to deform in the conveying and wetting and drying processes, so that strips generated by fluorescence reaction on the carrier collected by a camera device are deformed in the subsequent detection process, the comparative analysis is difficult, the accuracy of the detection result is influenced, and the carrier passing through the lower part of the leveling device can be flattened through the leveling device;
the excitation device 3 comprises a sliding main rod 31, a convex sliding rail is arranged on one side of the sliding main rod 31, the sliding main rod 31 is connected with two sliding blocks in a sliding manner through the sliding rail, one sliding block is fixedly connected with a light source fixing plate 33, the other sliding block is fixedly connected with a light-transmitting ring fixing plate 34, an excitation light source 35 is fixedly arranged on the light source fixing plate 33, and a circular light-transmitting ring 36 penetrating through the light-transmitting ring fixing plate 34 is arranged on the light-transmitting ring fixing plate 34; the light-transmitting ring 36 is arranged right below the light-emitting point of the excitation light source 35, and the irradiation range of the excitation light source 35 is limited by the arrangement of the light-transmitting ring fixing plate 34, so that the influence of reflection, diffusion and other factors on the excitation of the fluorescence reaction is reduced;
the excitation light source 35 is a multi-spectral L ED light source;
the camera device 4 is an industrial camera and is used for shooting the carrier passing through the camera device 4 to obtain a strip generated by a fluorescence reaction on the corresponding carrier and transmitting the shot picture to a corresponding analysis module for analysis and detection;
the method for detecting the up-conversion fluorescence by the up-conversion fluorescence detection device comprises the following steps:
s1, reacting the up-conversion fluorescent molecular probe with an object to be detected on a carrier, wherein the up-conversion fluorescent molecular probe is a material which is excited by high-energy light and emits low-energy light;
and S2, fixing the reacted carrier on a conveyor belt, driving the conveyor belt to rotate by the power device 2, transmitting the carrier to the position below the excitation light source 35, starting the excitation light source to irradiate the carrier for a preset time T, transmitting the carrier to the position near the camera device 4, and shooting by the camera device 4 to obtain a strip generated by the fluorescence reaction on the corresponding carrier.
The up-conversion fluorescence detection device can continuously perform fluorescence detection and has higher detection consistency and accuracy.
The foregoing is merely exemplary and illustrative of the present invention and various modifications, additions and substitutions may be made by those skilled in the art to the specific embodiments described without departing from the scope of the invention as defined in the following claims.
Claims (7)
1. The up-conversion fluorescence detection device is characterized by comprising a conveying device (1), wherein the conveying device (1) is driven by a power device (2), an excitation device (3) and a camera device (4) are arranged on the side edge of the conveying device (1), and a correction device (5) and a leveling device (6) are arranged at one end of the conveying device (1);
the conveying device (1) comprises a base platform (11), a light transmission groove (12) is formed in the middle of the base platform (11), a camera light transmission groove (13) is formed in the middle of the base platform (11), roller shafts (14) are rotatably arranged at two ends of the base platform (11), two ends of each roller shaft (14) are rotatably connected with the base platform (11) through bearings, and the two roller shafts (14) are connected through a conveying belt;
the excitation device (3) comprises a sliding main rod (31), a convex sliding rail is arranged on one side of the sliding main rod (31), the sliding main rod (31) is connected with two sliding blocks in a sliding mode through the sliding rail, one sliding block is fixedly connected with a light source fixing plate (33), the other sliding block is fixedly connected with a light-transmitting ring fixing plate (34), an excitation light source (35) is fixedly installed on the light source fixing plate (33), and a circular light-transmitting ring (36) penetrating through the light-transmitting ring fixing plate (34) is arranged on the light-transmitting ring fixing plate (34); the light-transmitting diaphragm (36) is arranged right below the light-emitting point of the excitation light source (35).
2. The up-conversion fluorescence detection device according to claim 1, wherein the power device (2) comprises a driving motor (21), a shaft extension end of the driving motor (21) is connected with a transmission shaft, a motor housing of the driving motor (21) is fixedly connected with a mounting frame (23) through a plurality of stabilizer bars (22), the mounting frame (23) is fixedly mounted on the frame, a gear is fixedly sleeved on the transmission shaft, gears are respectively sleeved at two ends of the roller shaft (14), and the gear on the roller shaft (14) is connected with the gear sleeved on the transmission shaft through a belt or a chain.
3. The up-conversion fluorescence detection device according to claim 1, wherein the correction device (5) comprises a main rod (51), two ends of the main rod (51) are rotatably connected with a threaded rod (52), a threaded sleeve (53) is sleeved on the threaded rod (52), the threaded sleeve (53) is in threaded fit with the threaded rod (52), the threaded sleeve (53) is further sleeved on the main rod (51), the threaded sleeve (53) is in sliding fit with the main rod (51), a mounting rod (54) is further fixedly mounted on the threaded sleeve (53), a mounting plate (55) is fixedly mounted on the mounting rod (54), a laser lamp bead (56) is fixedly mounted on the mounting plate (55), and a laser ray of the laser lamp bead (56) is projected to one point on the surface of the conveyor belt.
4. The up-conversion fluorescence detection device according to claim 1, wherein the leveling device (6) comprises a leveling main rod (61), an adjusting mounting plate (62) is fixedly mounted at the end of the leveling main rod (61) through a bolt, a strip-shaped hole is formed in the adjusting mounting plate (62), a mounting block (63) is fixedly mounted on the adjusting mounting plate (62) through a bolt, and the mounting block (63) is rotatably connected with one end of a pressing shaft (64).
5. The up-conversion fluorescence detection device according to claim 1, wherein the excitation light source (35) is a multi-spectral L ED light source.
6. The up-conversion fluorescence detection device according to claim 1, wherein the camera device (4) is an industrial camera.
7. The up-conversion fluorescence detection device according to claim 1, wherein the up-conversion fluorescence detection method by the up-conversion fluorescence detection device is:
s1, reacting the up-conversion fluorescent molecular probe with an object to be detected on a carrier, wherein the up-conversion fluorescent molecular probe is a material which is excited by high-energy light and emits low-energy light;
s2, fixing the reacted carrier on a conveyor belt, driving the conveyor belt to rotate by a power device (2), transmitting the carrier to the lower part of an excitation light source (35), starting the excitation light source to irradiate the carrier for a preset time T, then transmitting the carrier to the vicinity of an image pickup device (4), and shooting by the image pickup device (4) to obtain a strip generated by the fluorescence reaction on the corresponding carrier.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010276226.XA CN111413307A (en) | 2020-04-09 | 2020-04-09 | Up-conversion fluorescence detection device and detection method |
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| CN202010276226.XA CN111413307A (en) | 2020-04-09 | 2020-04-09 | Up-conversion fluorescence detection device and detection method |
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