CN111412860B - Observation objective table for laser confocal microscope biofilm - Google Patents
Observation objective table for laser confocal microscope biofilm Download PDFInfo
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- CN111412860B CN111412860B CN202010217455.4A CN202010217455A CN111412860B CN 111412860 B CN111412860 B CN 111412860B CN 202010217455 A CN202010217455 A CN 202010217455A CN 111412860 B CN111412860 B CN 111412860B
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- biofilm
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01B—MEASURING LENGTH, THICKNESS OR SIMILAR LINEAR DIMENSIONS; MEASURING ANGLES; MEASURING AREAS; MEASURING IRREGULARITIES OF SURFACES OR CONTOURS
- G01B11/00—Measuring arrangements characterised by the use of optical techniques
- G01B11/24—Measuring arrangements characterised by the use of optical techniques for measuring contours or curvatures
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01B—MEASURING LENGTH, THICKNESS OR SIMILAR LINEAR DIMENSIONS; MEASURING ANGLES; MEASURING AREAS; MEASURING IRREGULARITIES OF SURFACES OR CONTOURS
- G01B9/00—Measuring instruments characterised by the use of optical techniques
- G01B9/04—Measuring microscopes
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
- G01N21/8422—Investigating thin films, e.g. matrix isolation method
Abstract
The invention discloses a laser confocal microscope biofilm observation objective table, which comprises an objective table component and a biofilm attachment piece bearing component; the objective table subassembly includes the slide bar that two parallel intervals set up, and biofilm attachment piece bearing assembly includes the mobile station that two parallel intervals set up, the both ends of mobile station respectively with two slide bar sliding connection, the below of every mobile station all is provided with an adjusting block that can reciprocate, the outside one section distance that extends in side bottom that two adjusting blocks are relative forms the brace table that is used for placing biofilm attachment piece. The invention has simple structure, easy assembly and convenient operation, can solve the problems of fixation and support of the biofilm attachment piece during the observation of the laser confocal microscope in the experimental process, and can also be applied to other electron microscope platforms, such as an inverted microscope, a common optical microscope, a fluorescence microscope and the like.
Description
Technical Field
The invention belongs to the field of laser confocal electron microscope accessories, and particularly relates to a laser confocal electron microscope biofilm observation objective table.
Background
Biofilm (Biofilm) refers to a group of microorganisms which are attached to the surface of biological or non-biological materials, are wrapped by extracellular macromolecules secreted by the microorganisms and have certain three-dimensional structural characteristics, and researches show that bacteria and fungi can form the Biofilm, and the basic composition of the Biofilm comprises: the biofilm forming process comprises an initial colonization stage, an irreversible adhesion stage, a structural differentiation stage, a development and maturation stage, a final bacterial shedding and replanting stage and the like, and experimental data prove that each process is influenced by a plurality of factors, such as material properties of an attachment carrier, physicochemical properties of a cell surface, nutrition, environmental conditions and the like. The formation of the biofilm can cause the improvement of microbial drug resistance and other phenotypical changes different from the planktonic state of the biofilm, thereby causing a series of health and environmental problems, but at the same time, the biofilm is not a place at all, and can be developed and utilized, and the biofilm is widely applied to the environmental treatment fields of sewage treatment, heavy metal ion adsorption and the like.
In order to better control and utilize various biofilms, scientists all over the world have conducted a lot of researches on the biofilm formation process, gene expression, influencing factors, metabolic regulation and drug resistance mechanism, and during the researches, various electron microscopes (such as scanning electron microscope, projection electron microscope, fluorescence microscope, laser confocal scanning microscope, and the like) are often needed to observe the biofilm morphology so as to grasp the biofilm formation process and the morphology and changes of three-dimensional structure, while the biofilm in-vitro attachment carriers are usually glass sheets (such as laboratory glass slides and cover slips), iron sheets, plastic sheets, and the like, and usually, the attachment carriers such as cover slips are too thin or too small, while the stage configured by the electron microscope cannot support and fix the in a container (or on), because a physical barrier is additionally arranged between a sample and an objective lens, focusing is difficult, and therefore, the invention of the objective table capable of bearing the biofilm attachment sheet is urgently needed, so that the convenience and the accuracy of biofilm electron microscope observation (particularly laser confocal scanning microscope) are improved.
Disclosure of Invention
In view of the above problems, the present invention provides a confocal laser microscope biofilm observation stage with simple structure and convenient assembly.
In order to realize the purpose, the invention adopts the technical scheme that:
a laser confocal microscope biofilm observation objective table comprises an objective table component and a biofilm attachment piece bearing component; the objective table subassembly includes the slide bar that two parallel intervals set up, and biofilm attachment piece bearing assembly includes the mobile station that two parallel intervals set up, the both ends of mobile station respectively with two slide bar sliding connection, the below of every mobile station all is provided with an adjusting block that can reciprocate, the outside one section distance that extends in side bottom that two adjusting blocks are relative forms the brace table that is used for placing biofilm attachment piece.
As an improvement of the invention, the support table is provided with a clamping strip for clamping the biofilm attachment piece, a plurality of springs for adjusting the extending distance of the clamping strip are arranged between the clamping strip and the side surface of the adjusting block, and two ends of the mobile station are also provided with fixing screws for positioning the mobile station on the sliding strip.
Furthermore, the screens strip be soft rubber slab. Thus, the biofilm attaching plate can adapt to biofilm attaching plates with different shapes.
As an improvement of the invention, the mobile station drives the adjusting block to move up and down through a lifting screw in the middle, and limiting cylinders which face upwards and penetrate through the mobile station are arranged on two sides of the adjusting block.
Furthermore, the middle part of the adjusting block is provided with a stepped hole which is small at the top and big at the bottom, the bottom of the lifting screw is provided with a limiting clamp which is matched with the major diameter of the stepped hole, and the bottom end of the lifting screw is provided with a downward buffer spring. Thus, damage to the biofilm adhesion piece or the sample due to erroneous operation can be caused when the biofilm adhesion piece comes into contact with the objective lens of the confocal laser microscope.
As an improvement of the invention, the bottom of the sliding strip is provided with at least two suckers capable of sliding back and forth along the length direction of the sliding strip, and the suckers are used for fixing the sliding strip on an object stage of the laser confocal microscope. So, adopt the sucking disc to connect convenient operation, and through the interval of adjusting the sucking disc, the laser confocal microscope objective table of adaptable different specifications.
As an improvement of the invention, the object stage assembly further comprises two fixed stages which are arranged in parallel at intervals, and the sliding strip is respectively erected between the two fixed stages.
Furthermore, the bottom of the fixed platform is provided with at least two suckers capable of sliding back and forth along the length direction of the fixed platform, and the suckers are used for fixing the fixed platform on the objective table of the laser confocal microscope.
Furthermore, the two ends of the fixed station and the two ends of the mobile station are provided with clamping grooves, and the cross section of the sliding strip is consistent with the shape of the clamping grooves and is respectively arranged on the fixed station and the mobile station in a penetrating mode.
As an improvement of the invention, the sliding strip is provided with scales. In this way, it is convenient to adjust and control the distance between the mobile stations.
Compared with the prior art, the invention has the beneficial effects that:
the observation objective table is fixed on the original objective table of the laser confocal microscope, the position of the observation objective table can be adjusted up and down, back and forth and left and right through the position control knob of the laser confocal microscope, and the up and down, back and forth and left and right positions of the biofilm attachment piece can be adjusted through the biofilm attachment piece bearing assembly, so that the laser confocal microscope can focus quickly and observe the form of a biofilm conveniently.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this application, illustrate embodiments of the application and, together with the description, serve to explain the application and are not intended to limit the application.
FIG. 1 is a schematic view of an assembled view of an observation stage according to the present invention;
FIG. 2 is a schematic view of an exploded view of the viewing stage of the present invention;
FIG. 3 is a schematic structural view of a biofilm carrier assembly;
FIG. 4 is a schematic view of the structure of the fixing table at both ends;
fig. 5 is a schematic view of the structure of the slider.
Description of reference numerals: 1-a mobile station; 2-a regulating block; 3-lifting screws; 4-fixing screws; 5-a limiting cylinder; 6-clamping strip; 7-biofilm attachment sheets; 8-a spring; 9-a buffer spring; 10-a card slot; 11-a stepped hole; 12-a stationary table; 13-a suction cup; 14-a track; 15-a slide bar; 16-graduated scale.
Detailed Description
Technical solutions in one or more embodiments of the present disclosure will be clearly and completely described below with reference to the accompanying drawings in one or more embodiments of the present disclosure, and it is apparent that the described embodiments are only a part of the embodiments of the present disclosure, and not all embodiments. All other embodiments, which can be derived by one of ordinary skill in the art from one or more embodiments of the disclosure without making any creative effort, shall fall within the scope of protection of the disclosure.
As shown in fig. 1 to 5, the present embodiment discloses a confocal laser microscope biofilm observation stage, which includes a stage assembly and a biofilm attachment wafer bearing assembly, wherein the biofilm attachment wafer bearing assembly is used for bearing, positioning and adjusting a biofilm attachment wafer 7, and the stage assembly is used for positioning the biofilm attachment wafer bearing assembly on an original stage of a confocal laser microscope. It is understood that the observation stage of the present invention can be applied to other electron microscope platforms, such as an inverted microscope, a general optical microscope, a fluorescence microscope, etc., besides the confocal laser microscope.
The objective table assembly comprises two fixed tables 12 arranged in parallel at intervals and two sliding strips 15 arranged in parallel at intervals, two ends of each fixed table 1 are provided with grooves 10, the shapes of the grooves 10 are consistent with those of the sliding strips 15, and when the fixed tables 12 and the sliding strips 15 are assembled together, a rectangular frame is formed. In order to realize the disassembly and assembly with the original objective table of the confocal laser microscope, the bottom of the fixed table 12 is provided with a track 14, two suckers 13 capable of sliding along the track 14 are arranged on the track 14, the suckers 13 can realize the sliding connection with the track 14 through sliders, the specific structure is the prior art, and details are not repeated herein. Adopt sucking disc 13 fixed, for connected modes such as screw thread, buckle, have simple structure, easy dismounting's advantage, simultaneously, because sucking disc 13 can slide along track 14, can adjust according to the size of the original objective table of confocal laser microscope, realize the cooperation with the original objective table of confocal laser microscope of unidimensional not.
Biofilm attachment piece carrier assembly includes two parallel mobile station 1 that the interval set up, and the both ends of mobile station 1 all are provided with recess 10 for mobile station 1 makes a round trip to slide on two slide bars 15. In order to position the mobile station 1 on the slide bar 15, a fixing screw 4 can be arranged at the position of the groove 10 of the mobile station 1, and the fixing and the position adjustment of the mobile station 1 can be realized by screwing the fixing screw 4. In order to adjust the position of the mobile stations 1 more accurately, the slide bar 15 is provided with a scale 16, so that the distance between the two mobile stations 1 can be easily adjusted according to the width of the biofilm attachment pieces 7. An adjusting block 2 is arranged below each mobile station 1, and the bottoms of the opposite side surfaces of the two adjusting blocks 2 extend outwards for a certain distance to form a supporting table for placing a biofilm attachment sheet 7. In order to adjust the height of biofilm attachment piece 7, the middle part threaded connection of mobile station 1 has lifting screw 3, the middle part of regulating block 2 is provided with big end down's shoulder hole 11, lifting screw 3's bottom is provided with the spacing card that matches with shoulder hole 11 major diameter, after lifting screw 3 inserts shoulder hole 11, lifting screw 3 can 360 rotatory nevertheless can not extract from shoulder hole 11 again, so, can drive regulating block 2 up-and-down motion through rotating lifting screw 3, thereby adjust biofilm attachment piece 7's height. In order to ensure that the adjusting block 2 integrally moves vertically up and down, two sides of the adjusting block 2 are provided with limiting cylinders 5 which face upwards and penetrate through the mobile station 1.
In order to realize the good location of biofilm attachment piece 7, the screens strip 6 that is provided with on the brace table to interval is provided with a plurality of springs 8 between screens strip 6 and regulating block 2 side, and like this, after biofilm attachment piece 7 was placed on the brace table, screens strip 6 will chucking biofilm attachment piece 7 under the effect of spring 8 elasticity, avoids biofilm attachment piece 7 to produce the removal in the observation process. Preferably, the position-limiting strip 6 is made of soft rubber plate, and can be well attached to the edge of the irregular attachment piece through matching with the spring 8, so that the position-limiting strip can be used for fixing the biofilm attachment pieces 7 with different shapes.
In addition, in the process of adjusting the position of the biofilm attachment piece 7, misoperation (the object lens is lifted too high) that the biofilm attachment piece 7 is in over contact with the objective lens of the laser confocal microscope can occur, in order to avoid damage to the attachment piece or the sample caused by the misoperation, the buffer spring 9 is arranged at the bottom of the lifting screw 3, so that the impact and damage of the objective lens of the laser confocal microscope to the biofilm attachment piece 7 can be buffered, and the sample can be better protected.
It should be noted that although the stage assembly includes the fixed stage 12 and the slide bar 15 in the embodiment, the function of the fixed stage 12 can be integrated into the slide bar 15, for example, the slide bar 15 does not pass through the movable stage 1, but the slide rail is set on the top surface to match with the movable stage 1, and meanwhile, the bottom structure of the slide bar 15 is the same as the fixed stage 12 and is connected with the original stage of the confocal laser microscope through the suction cup 13.
The observation objective table of the invention has the following use process:
the mobile station 1 and the adjusting block 2 and the related connecting elements are assembled, then the sliding strip 15 with the scale 16 is inserted into the slot 10 of the mobile station 1 and then inserted into the slot 10 of the fixed station 12, so that the mobile station 1 and the fixed station 12 are integrated into a whole and are fixed by the fixing screw 4 as required (the fixing screw 4 can also be arranged at the position of the groove 10 of the fixed station 12, although not shown in the figure).
Then, according to the scale 16 and the width of the biofilm attachment piece 7, the component of the two mobile stations 1 is adjusted to a proper distance, the biofilm attachment piece 7 is placed in the middle of the clamping strip 6, and the clamping strip 6 is ensured to apply a certain pressure on the biofilm attachment piece 7.
Finally, the assembled observation objective table and the biofilm attachment piece 7 thereof are fixed on the original objective table of the laser confocal microscope by a sucker 13, the lifting screw 3 is rotated to keep the biofilm attachment piece 7 and the objective lens of the laser confocal microscope at a proper distance or in contact, and then the observation objective table of the invention is controlled by using the front-back, left-right and up-down moving knobs of the laser confocal microscope to carry out the rapid focusing of the laser confocal microscope and the observation of the form of the biofilm.
The above embodiments are only for illustrating the technical concept and features of the present invention, and the purpose thereof is to enable those skilled in the art to understand the contents of the present invention and implement the present invention accordingly, and not to limit the protection scope of the present invention accordingly. All equivalent changes or modifications made in accordance with the spirit of the present disclosure are intended to be covered by the scope of the present disclosure.
Claims (9)
1. A laser confocal microscope biofilm observation objective table is characterized in that: comprises an object stage assembly and a biofilm attachment wafer bearing assembly; objective table subassembly includes the slide bar that two parallel intervals set up, biofilm attachment piece bearing assembly includes the mobile station that two parallel intervals set up, the both ends of two mobile stations respectively with two slide bar sliding connection, the below of every mobile station all is provided with a regulating block that can reciprocate, the outside one section distance that extends in side bottom that two regulating blocks are relative forms the brace table that is used for placing the biofilm attachment piece, be provided with the screens strip that is used for chucking biofilm attachment piece on the brace table, the screens strip is soft rubber plate, be provided with a plurality of springs that are used for adjusting screens strip distance of stretching out between screens strip and the regulating block side.
2. The confocal laser microscopy biofilm observation stage of claim 1, wherein: and fixing screws are further arranged at two ends of the mobile station and used for positioning the mobile station on the sliding strip.
3. The confocal laser microscopy biofilm observation stage of claim 1, wherein: the mobile station drives the adjusting block to move up and down through the lifting screw in the middle, and limiting cylinders which face upwards and penetrate through the mobile station are arranged on two sides of the adjusting block.
4. The confocal laser microscopy biofilm observation stage according to claim 3, wherein: the middle part of regulating block be provided with big end down's shoulder hole, the bottom of lifting screw is provided with the spacing card that matches with the shoulder hole major diameter, the bottom of lifting screw is provided with buffer spring down.
5. The confocal laser microscopy biofilm observation stage of claim 1, wherein: the bottom of the sliding strip is provided with at least two suckers capable of sliding back and forth along the length direction of the sliding strip, and the suckers are used for fixing the sliding strip on an objective table of the laser confocal microscope.
6. The confocal laser microscopy biofilm observation stage of claim 1, wherein: the objective table assembly further comprises two fixing tables which are arranged in parallel at intervals, and the sliding strip is respectively erected between the two fixing tables.
7. The confocal laser microscopy biofilm observation stage of claim 6, wherein: the bottom of the fixed table is provided with at least two suckers capable of sliding back and forth along the length direction of the fixed table and used for fixing the fixed table on an object stage of the laser confocal microscope.
8. The confocal laser microscopy biofilm observation stage of claim 7, wherein: the two ends of the fixed station and the two ends of the mobile station are both provided with clamping grooves, and the cross section of the sliding strip is consistent with the shape of the clamping grooves and is respectively arranged on the fixed station and the mobile station in a penetrating mode.
9. The confocal laser microscopy biofilm observation stage of claim 1, wherein: the sliding strip is provided with scales.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010217455.4A CN111412860B (en) | 2020-03-25 | 2020-03-25 | Observation objective table for laser confocal microscope biofilm |
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| CN202010217455.4A CN111412860B (en) | 2020-03-25 | 2020-03-25 | Observation objective table for laser confocal microscope biofilm |
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| CN111412860B true CN111412860B (en) | 2022-03-22 |
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