CN111410303A - Rapid forming method for coupling denitrification anaerobic methane oxidation and anaerobic ammonia oxidation to granular sludge - Google Patents
Rapid forming method for coupling denitrification anaerobic methane oxidation and anaerobic ammonia oxidation to granular sludge Download PDFInfo
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Abstract
A rapid forming method of denitrification anaerobic methane oxidation and anaerobic ammonia oxidation coupled granular sludge is characterized in that anaerobic ammonia oxidation granular sludge rapidly adsorbs flocculent denitrification anaerobic methane oxidation microorganisms, 3-oxo-C6-HS L and DPD are added to promote the field planting of the anaerobic methane oxidation microorganisms on the surface of the anaerobic ammonia oxidation granular sludge, and then sufficient soluble CH is ensured by continuous flow culture4In the existing method, the rapid growth and metabolic activity of denitrifying anaerobic methane-oxidizing microorganisms are enhanced by adopting high-concentration ammonia nitrogen and nitrate concentration, 3-oxo-C6-HS L, C6-HS L, C12-HS L and DPD are added to enhance the rapid growth of anaerobic methane-oxidizing archaea and bacteria, and Ca is added2+And Sr2+The mixed ions strengthen the action of a second messenger of the anaerobic methane oxidation archaea, promote the rapid growth and film formation of the anaerobic methane oxidation archaea, strengthen the stability and the particle strength of the coupling particles, and further promote the rapid formation of the coupling particles.
Description
Technical Field
The invention belongs to the technical field of sewage treatment, and particularly relates to a rapid forming method of granular sludge by coupling denitrification anaerobic methane oxidation and anaerobic ammonia oxidation.
Background
Along with the development of production and life of people, a large amount of nitrogen-containing wastewater is generated, and if the nitrogen-containing wastewater is directly discharged without treatment, serious harm is caused to the environment. The nitrogenous substances can not only cause water eutrophication, but also seriously damage the aquatic ecosystem; after entering the ground water, NO3 -Or NO2 -Excessive intake can also cause "blue baby disease". The existing denitrification technology mainly comprises three methods, namely a physical method, a chemical method and a biological method, and compared with the former two methods, the biological denitrification technology has the advantages of mild conditions, low treatment cost, high denitrification efficiency and the like, so that the biological denitrification technology is favored by people.
Among the biological denitrification technologies, the anaerobic ammonia oxidation technology is praised as one of the most economical denitrification technologies at present because of the advantages of no need of aeration, no need of adding organic matters, low yield of residual sludge and the like. The technology can simultaneously convert nitrite and ammonia nitrogen into N under anaerobic condition2The method has the advantages of low operation cost, economy, high efficiency, no secondary pollutants and the like. However, according to the reaction mechanism of anaerobic ammonia oxidation, 21% of nitrite nitrogen is converted into nitrate nitrogen by anaerobic ammonia oxidation reaction, so that the total nitrogen removal rate is only about 80% theoretically; in practical application, the total nitrogen removal rate can only be maintained at about 70 percent due to the non-ideal proportion of the nitrite and the ammonia nitrogen in the inlet water.
Denitrifying anaerobic methane-oxidizing microorganisms discovered in recent years comprise two main classes of denitrifying anaerobic methane-oxidizing archaea and denitrifying anaerobic methane-oxidizing bacteria; wherein the denitrifying anaerobic methane-oxidizing archaea can be CH4As an electron donor, converting nitrate to nitrite; while the denitrifying anaerobic methane-oxidizing bacteria can react with CH4Nitrite is converted to nitrogen for the electron donor. Coupling of denitrifying anaerobic methane-oxidizing microorganisms and anaerobic ammonium-oxidizing bacteriaBy the synergistic effect, nitrate nitrogen generated by anaerobic ammonia oxidation reaction can be effectively converted, and the total nitrogen removal rate is improved; the denitrifying anaerobic methane-oxidizing bacteria can also effectively remove nitrite nitrogen in the inlet water, adjust the proportion of inlet water ammonia nitrogen and nitrite nitrogen and promote anaerobic ammoxidation; at the same time, the denitrifying anaerobic methane-oxidizing microorganisms can also remove the soluble CH in the influent water4The method has the function of synchronously removing greenhouse gases such as methane and nitrogen-containing pollutants.
However, the denitrifying anaerobic methane-oxidizing archaea, the denitrifying anaerobic methane-oxidizing bacteria and the anaerobic ammonia-oxidizing bacteria are autotrophic bacteria and have the defects of slow growth, long multiplication time and the like; the synergistic action state of denitrifying anaerobic methane-oxidizing microorganisms and anaerobic ammonium-oxidizing bacteria in the existing research mostly exists in the form of flocculent sludge, the sedimentation effect is poor, and functional microorganisms are easy to lose. Compared with the existing form of granular sludge, the existing form of granular sludge has the advantages of high sludge concentration, high sedimentation speed, high stability and the like, and the synergistic effect of the denitrifying anaerobic methane-oxidizing microorganisms and the anaerobic ammonia-oxidizing bacteria in the form has greater application potential.
The patent 201711472212 to Xie Jun et al provides a novel membrane aeration anaerobic granular sludge reactor and a high-efficiency denitrification and greenhouse gas emission reduction method thereof, and solves the problems of low solubility of gas substances in an aqueous solution, large gas-liquid mass transfer resistance and low mass transfer rate. The method takes flocculent sludge and anaerobic granular sludge which are subjected to enrichment culture and contain denitrifying anaerobic methane-oxidizing bacteria, denitrifying anaerobic methane-oxidizing archaea and anaerobic ammonia oxidation functional microorganisms as inoculation sludge, and forms granular sludge rich in denitrifying anaerobic methane-oxidizing bacteria and anaerobic ammonia oxidation bacteria after a novel membrane aeration anaerobic granular sludge reactor operates for 100 days, wherein the granular sludge is circular or ellipsoidal, and the particle size is 500-1000 microns.
CN101058463A discloses a biological wastewater treatment method for realizing anaerobic ammonia oxidation and methanation denitrification coupling, which comprises the steps of inoculating granular sludge with methanation, denitrification and anaerobic ammonia oxidation activities in an Expanded Granular Sludge Bed (EGSB) reactor, inoculating aerobic ammonia oxidizing bacteria in a Biological Aerated Filter (BAF) or SHARON process or SBR reactor, connecting the Expanded Granular Sludge Bed (EGSB) reactor with the Biological Aerated Filter (BAF) or SHARON process or SBR reactor, and successfully realizing biological wastewater treatment of anaerobic ammonia oxidation and methanation denitrification coupling by reasonably controlling pH value, temperature, dissolved oxygen, external reflux ratio, load rate, redox potential, organic matter concentration and ammonia nitrogen concentration. The technology can synchronously remove organic COD and inorganic ammonia components at a high volume load rate, simultaneously save a considerable part of energy and organic carbon source consumption, and can be applied to the biological treatment of common urban sewage and industrial wastewater.
Disclosure of Invention
Based on the method, the invention provides a rapid forming method of the denitrification anaerobic methane oxidation and anaerobic ammonia oxidation coupled granular sludge, which can greatly shorten the culture time of the denitrification anaerobic methane oxidation and anaerobic ammonia oxidation coupled granular sludge, and can effectively solve the problems of poor sludge settling effect, easy loss of functional microorganisms and the like in the existing technology of coupling denitrification anaerobic methane oxidation microorganisms and anaerobic ammonia oxidizing bacteria.
Based on one aspect of the invention, the invention discloses a rapid forming method of denitrification anaerobic methane oxidation and anaerobic ammonia oxidation coupled granular sludge, which comprises the following steps of (1) adopting an upflow anaerobic sludge bed reactor, taking flocculent sludge containing denitrification anaerobic methane oxidation bacteria and denitrification anaerobic methane oxidation archaea which are subjected to enrichment culture and mixed sludge formed by anaerobic ammonia oxidation granular sludge as inoculum, controlling the temperature to be 25-35 ℃, the reactor rising flow rate to be 3-5m/h, intermittently adding nitrate concentrated solution, nitrite concentrated solution and ammonia nitrogen concentrated solution for culture, ensuring that the nitrite concentration in the system is maintained below 20 mgN/L, the ammonia nitrogen concentration and the nitrate concentration are both below 100 mgN/L, and intermittently aerating CH4Gas to ensure the concentration of the dissolved methane in the system to be more than 10 mg/L, simultaneously adding 1-5 mu mol/L of 3-oxo-C6-HS L (N-3-oxo-hexanoyl homoserine lactone) and 10-50 ng/L of DPD (4, 5-dihydroxy-2, 3-pentanedione) to promote the permanent planting of denitrifying anaerobic methane oxidation microorganisms on the surface of anaerobic ammonia oxidation granular sludge, and carrying out the step after 3-14 days of intermittent cultureStep (2); (2) continuous flow influent water containing dissolved CH4Nitrate and ammonia nitrogen, the CH4The concentration of the sodium nitrate is 15-20 mg/L, the concentration of the nitrate is maintained at 100-800 mgN/L, the concentration of the ammonia nitrogen is 100-800 mgN/L, the water conservation retention time is maintained at 4-24 h, 3-oxo-C6-HS L (N-3-oxo-hexanoyl homoserine lactone), C6-HS L (N-hexanoyl-L-homoserine lactone) and C12-HS L (N-dodecanoyl-L-homoserine lactone) with the concentrations of 2-5 mu mol/L and DPD with the concentration of 20-100 ng/L are added, and Ca is added simultaneously2+And Sr2+The molar ratio of the mixed ions to the counter ions is 1:1-2:1, the concentration is 2-5 mu mol/L, so that the activity of the denitrifying anaerobic methanogens is improved, the rapid growth and film formation of the denitrifying anaerobic methanogens are promoted, the rapid formation of the coupling granular sludge is promoted, and the coupling granular sludge culture is completed after the continuous culture for 30-90 days.
The intermittent addition of the nitrate concentrated solution, the nitrite concentrated solution and the ammonia nitrogen concentrated solution in the step (1) is carried out according to the following steps that when the concentration of nitrite in the system is below 5 mg/L and the concentration of nitrate and ammonia nitrogen is below 10 mg/L, the nitrite concentrated solution, the nitrate concentrated solution and the ammonia nitrogen concentrated solution are respectively injected by adopting an injector in a pulse mode.
The mass ratio of the flocculent sludge containing the denitrifying anaerobic methane-oxidizing bacteria and the denitrifying anaerobic methane-oxidizing archaea and the anaerobic ammonia oxidation granular sludge which are subjected to enrichment culture in the step (1) is 1:1-5: 1-3;
the reactor upflow rate in step (1) is preferably 2.5 to 3.0, more preferably 2.8;
the concentrations of C3-oxo-C6-HS L, C6-HS L, C12-HS L0 and DPD in the step (2) are preferably 3-4 mu mol/L, 2-3 mu mol/L, 3-4 mu mol/L and 40-60 ng/L, more preferably 3.5 mu mol/L, 2.5 mu mol/L, 3.5 mu mol/L and 50 ng/L;
ca in step (2)2+And Sr2+The preferred adding mode of the mixed ions is that the continuous flow is started in the initial stage according to Ca2+And Sr2+The molar ratio is 1:1.5-3, and Ca is changed when the EPS increase reaches 40 percent2+And Sr2+The molar ratio is 1: 0.5-1.5.
In another aspect of the invention, the granular sludge cultured by the rapid formation method of the denitrification anaerobic methane oxidation and anaerobic ammonia oxidation coupled granular sludge is disclosed, and the particle size of the granular sludge is 0.4-2.0 mm; the particles are round or ellipsoidal, and extracellular polymers are distributed on the surfaces of the particles; the denitrifying anaerobic methane archaea and the denitrifying anaerobic methane oxidizing bacteria are positioned outside the granular sludge, and the anaerobic ammonia oxidizing bacteria are positioned inside the granular sludge.
The main denitrifying anaerobic methane-oxidizing archaea is Candidatus Methanoperedens; the main denitrifying anaerobic methane-oxidizing bacteria are Candidatus Methylomirabilis; the main anammox bacterium is Candidatus Brocadia;
the final nitrate consumption rate of the granular sludge is 50-150 mgN/L/d, the ammonia nitrogen consumption rate is 200-1000 mgN/L/d, the denitrification load reaches 500-2000 mgN/L/d, and the concentration of the granular sludge in the reactor is 5-50 gVSS/L.
The method adopts mixed sludge of activated sludge and anaerobic ammonium oxidation granular sludge formed by denitrifying anaerobic methane-oxidizing microorganisms as an inoculum, simultaneously carries out intermittent culture, utilizes the ecological compatibility of anaerobic ammonium oxidation bacteria to the denitrifying anaerobic methane-oxidizing microorganisms to quickly adsorb the denitrifying anaerobic methane-oxidizing microorganisms and avoid the loss of functional microorganisms, adds signal molecules 3-oxo-C6-HS L and DPD of the denitrifying anaerobic methane-oxidizing archaea and the denitrifying anaerobic methane-oxidizing bacteria to promote the permanent planting of the anaerobic methane-oxidizing microorganisms on the surface of the anaerobic ammonium oxidation granular sludge so as to form a primary coupled granular sludge embryonic form, and ensures sufficient dissolubility CH during continuous flow operation4During the process, high ammonia nitrogen and nitrate concentration is adopted to strengthen the rapid growth and metabolic activity of denitrifying anaerobic methane oxidation archaea, signal molecules of 3-oxo-C6-HS L, C6-HS L, C12-HS L and DPD are added to strengthen the rapid growth of anaerobic methane oxidation archaea, and Ca is added simultaneously2+And Sr2+The mixed ions strengthen the action of a second messenger of the anaerobic methane oxidation archaea and promote the rapid growth and film formation of the anaerobic methane oxidation archaea; ca2+And Sr2+The addition of (2) can also promote the generation of EPS, and strengthen the stability and the particle strength of the coupling particles, thereby promoting the rapid formation of the coupling particles.
The method has the advantages that the coupling granular sludge can be quickly formed, the formation time of a coupling granular sludge prototype is only about 7 days, the formation time of mature coupling granular sludge is only about 50 days, the obtained coupling granular sludge is high in stability and sludge concentration, the coupling granular sludge is good in treatment effect, the final consumption rate of nitrate and ammonia nitrogen reaches a higher level, the denitrification load can reach 1250 mgN/L/d at most, the particle size is moderate and is uniformly distributed, the coupling granular sludge can fully utilize methane gas generated in a sewage treatment plant, and greenhouse gases such as methane and nitrogen-containing pollutants can be synchronously removed.
Drawings
FIG. 1 is an optical photograph of coupled granular sludge grown in example eight;
FIG. 2 is an optical micrographs of coupled granular sludge cultured in example eight.
Detailed Description
The technical solution of the present invention is not limited to the following specific embodiments, but includes any combination between the specific embodiments.
Example 1
A method for quickly forming granular sludge by coupling denitrification anaerobic methane oxidation and anaerobic ammonia oxidation comprises (1) adopting an upflow anaerobic sludge bed reactor, taking mixed sludge containing denitrification anaerobic methane oxidation bacteria, denitrification anaerobic methane oxidation archaea flocculent sludge and anaerobic ammonia oxidation granular sludge which are subjected to enrichment culture as inoculum, controlling the temperature to be about 30 ℃, controlling the rising flow rate of the reactor to be 3-5m/h, intermittently adding nitrate concentrated solution, nitrite concentrated solution and ammonia nitrogen concentrated solution for culture, ensuring that the concentration of nitrite in the system is kept below 20 mgN/L and the concentration of ammonia nitrogen and nitrate are below 100 mgN/L, and intermittently aerating CH by adopting an aeration head4Gas to ensure the concentration of the dissolved methane in the system to be more than 10 mg/L, simultaneously adding 2 mu mol/L of 3-oxo-C6-HS L and 20 ng/L of DPD to promote the field planting of anaerobic methane oxidation microorganisms on the surface of anaerobic ammonia oxidation granular sludge, finishing the intermittent culture after 7 days, and (2) continuously flowing water, feeding water with soluble CH4The concentration is 15-20 mg/L, and the nitrate is concentratedKeeping the concentration at 530 mgN/L, the ammonia nitrogen concentration at 500 mgN/L, the water conservancy retention time at 4h-24h, adding 3-oxo-C6-HS L, C6-HS L, C12-HS L and DPD with the concentration of 2-5 mu mol/L respectively and 20-100 ng/L, and simultaneously adding Ca2+And Sr2+The molar ratio of the mixed ions to the counter ions is 1:1-2:1, the concentration is 2-5 mu mol/L, so that the activity of the denitrifying anaerobic methanogens is improved, the rapid growth and film formation of the denitrifying anaerobic methanogens are promoted, the rapid formation of the coupling granular sludge is promoted, and the coupling granular sludge culture is completed after continuous culture for 50 days.
Example 2
The difference between the embodiment and the first embodiment is that the reactor upflow speed in the first step is 3.5m/h, the concentrations of C3-oxo-C6-HS L, C6-HS L, C12-HS L and DPD in the second step are 3.5 mu mol/L, 2.5 mu mol/L, 3.5 mu mol/L and 40 ng/L, and other steps and parameters are the same as those in the first embodiment.
Example 3
The first difference between the present embodiment and the specific embodiment is: ca in step two2+And Sr2+The adding mode of the mixed ions is that Ca is adopted at the initial stage of continuous flow starting2+And Sr2+The molar ratio is 1:2, and the ratio is changed to Ca when the EPS increase reaches 40 percent2+And Sr2+The molar ratio is 1:1. Other steps and parameters are the same as those in the first embodiment.
Example 4
The difference between the first embodiment and the second embodiment is that the nitrite concentration in the system in the first step is maintained at 10 mgN/L, the ammonia nitrogen and nitrate concentration are both 100 mgN/L, and the soluble CH in the system in the second step4The concentration is 18 mg/L, the concentration of ammonia nitrogen and nitrate is 700 mgN/L, and other steps and parameters are the same as those of the first embodiment.
Example 5
In the first step, the reactor upflow rate is 3.8m/h, and in the second step, the concentrations of C3-oxo-C6-HS L, C6-HS L, C12-HS L and DPD are 3.8 mu mol/L, 2.8 mu mol/L, 3.5 mu mol/L and 60 ng/L.
Example 6
In the step one, the nitrite concentration in the system is maintained at 15 mgN/L, the concentration of ammonia nitrogen and nitrate is both 80 mgN/L, and the solubility CH in the system in the step two4The concentration was 19 mg/L other steps and parameters were the same as in the first embodiment.
Example 7
The nitrite concentration in the system in the first step is maintained at 14 mgN/L, the ammonia nitrogen and nitrate concentration in the system in the second step are both 800 mgN/L, and other steps and parameters are the same as those in the first embodiment.
Example 8
The method for quickly forming the denitrification anaerobic methane oxidation and anaerobic ammonia oxidation coupled granular sludge comprises the following steps of (1) adopting an up-flow anaerobic sludge bed reactor, taking mixed sludge which is prepared by enrichment culture and contains denitrification anaerobic methane oxidation bacteria, denitrification anaerobic methane oxidation archaea flocculent sludge and anaerobic ammonia oxidation granular sludge as an inoculum, controlling the temperature to be about 30 ℃, controlling the rising flow rate of the reactor to be 4m/h, intermittently adding nitrate concentrated solution, nitrite concentrated solution and ammonia nitrogen concentrated solution for culture, ensuring that the nitrite concentration in the system is maintained at 18 mgN/L, ensuring that the ammonia nitrogen and nitrate concentration are both 90 mgN/L, and intermittently aerating CH4Gas to ensure the concentration of the dissolved methane in the system to be 10 mg/L, 2 mu mol/L of 3-oxo-C6-HS L and 20 ng/L of DPD are added simultaneously to promote the permanent planting of denitrifying anaerobic methane oxidation microorganisms on the surface of anaerobic ammonium oxidation granular sludge, and the intermittent culture is finished after 7 days, (2) continuous flow water inlet is carried out, and water inlet solubility CH is carried out4The concentration is 17 mg/L, the nitrate concentration is kept at 530 mgN/L, the ammonia nitrogen concentration is 500 mgN/L, the water conservancy retention time is kept at 12h, 3-oxo-C6-HS L, C6-HS L, C12-HS L and DPD of 40 ng/L with the concentrations of 3.5 mu mol/L are added respectively, and simultaneously Ca is added2+And Sr2+The molar ratio of the mixed ions to the mixed ions is 2:1, and the concentrations of the mixed ions are 4 mu mol/L and 2 mu mol/L respectively, so that the activity of the denitrifying anaerobic methanogens is improved, the rapid growth and film formation of the denitrifying anaerobic methanogens are promoted, the rapid formation of the coupling granular sludge is promoted, and the coupling granular sludge culture is completed after continuous culture for 50 days.
After 50 days of culture, the concentration of the granular sludge is 15 gVSS/L, the particle size of the coupled granular sludge is 0.4-2.0mm, the particles are circular or ellipsoidal, extracellular polymers are distributed on the surfaces of the particles, denitrifying anaerobic methane archaea and denitrifying anaerobic methane oxidizing bacteria are positioned outside the granular sludge, anaerobic ammonium oxidation bacteria are positioned inside the granular sludge, the main denitrifying anaerobic methane archaea is Candidatus Methanouperdens, the main denitrifying anaerobic methane bacteria is Candidatus Methylornibilis, the main anaerobic ammonium oxidation bacteria is Candidatus Brocadia, the final nitrate consumption rate of the coupled particles is 95.2 mgN/L/d, the ammonia nitrogen consumption rate is 650 mgN/L/d, and the denitrification load is 1250N/L/mgN.
The above examples are illustrative of the present invention and are not intended to limit the scope of the present invention. All other solutions, which can be obtained by a person skilled in the art without any creative effort based on the described embodiments, belong to the protection scope of the present invention.
Claims (9)
1. A method for quickly forming granular sludge by coupling denitrification anaerobic methane oxidation and anaerobic ammonia oxidation comprises (1) adopting an upflow anaerobic sludge bed reactor, taking mixed sludge formed by flocculent sludge containing denitrification anaerobic methane oxidation bacteria and denitrification anaerobic methane oxidation archaea and anaerobic ammonia oxidation granular sludge which are subjected to enrichment culture as an inoculum, controlling the temperature to be 25-35 ℃, controlling the rising flow rate of the reactor to be 3-5m/h, intermittently adding nitrate concentrated solution, nitrite concentrated solution and ammonia nitrogen concentrated solution for culture, ensuring that the concentration of nitrite in the system is kept below 20 mgN/L, the concentration of ammonia nitrogen and nitrate is below 100 mgN/L, and intermittently aerating CH4Gas, ensuring the concentration of the dissolved methane in the system to be more than 10 mg/L, simultaneously adding 1-5 mu mol/L of 3-oxo-C6-HS L (N-3-oxo-hexanoyl homoserine lactone) and 10-50 ng/L of DPD (4, 5-dihydroxy-2, 3-pentanedione) to promote the permanent planting of denitrifying anaerobic methane oxidation microorganisms on the surface of anaerobic ammonia oxidation granular sludge, performing intermittent culture for 3-14 days, entering the step (2), and continuously feeding water in the step (2), wherein the fed water contains the dissolved CH4Nitrate and ammonia nitrogen, the CH4The concentration of the nitrate is 15-20 mg/L, the concentration of the nitrate is maintained at 100-800 mgN/L, the concentration of the ammonia nitrogen is 100-800 mgN/L, the water conservancy residence time is maintained at 4-24 h, and the adding concentration is 2-5 mu mo respectivelyl/L of 3-oxo-C6-HS L (N-3-oxo-hexanoyl homoserine lactone), C6-HS L (N-hexanoyl-L-homoserine lactone) and C12-HS L (N-dodecanoyl-L-homoserine lactone), and DPD of 20-100 ng/L, and adding Ca at the same time2+And Sr2+The molar ratio of the mixed ions to the mixed ions is 1:1-2:1, the concentration is 2-5 mu mol/L, so that the activity of the denitrifying anaerobic methane-oxidizing archaea is improved, the rapid growth and film formation of the denitrifying anaerobic methane-oxidizing archaea are promoted, the rapid formation of the coupled granular sludge is promoted, and the coupled granular sludge is cultured for 30-90 days to finish the culture of the coupled granular sludge.
2. The process according to claim 1, wherein the reactor upflow rate in step (1) is in the range of 2.5 to 3.0, more preferably 2.8.
3. The method according to claim 1, wherein the intermittently fed nitrate concentrated solution, nitrite concentrated solution and ammonia nitrogen concentrated solution in the step (1) are cultured by injecting the nitrite concentrated solution, the nitrate concentrated solution and the ammonia nitrogen concentrated solution respectively by using an injector in a pulse manner when the concentration of the nitrite in the system is below 5 mg/L and the concentration of the nitrate and the ammonia nitrogen is below 10 mg/L.
4. The process according to claim 1, wherein the concentrations of C3-oxo-C6-HS L, C6-HS L, C12-HS L and DPD in step (2) are preferably 3-4. mu. mol/L, 2-3. mu. mol/L, 3-4. mu. mol/L and 40-60 ng/L.
5. The method according to claim 1, wherein Ca is contained in the step (2)2+And Sr2+The adding mode of the mixed ions is that Ca is adopted at the initial stage of continuous flow starting2+And Sr2+The molar ratio is 1:1.5-3, and Ca is changed when the EPS increase reaches 40 percent2+And Sr2+The molar ratio is 1: 0.5-1.5.
6. The granular sludge cultured by the method according to any one of claims 1 to 5, having a particle size of 0.4 to 2.0 mm; the particles are round or ellipsoidal, and extracellular polymers are distributed on the surfaces of the particles; the denitrifying anaerobic methane archaea and the denitrifying anaerobic methane oxidizing bacteria are positioned outside the granular sludge, and the anaerobic ammonia oxidizing bacteria are positioned inside the granular sludge.
7. The granular sludge of claim 6 wherein the predominant denitrifying anaerobic methanogen archaea is Candidatus Methanoperedens; the main denitrifying anaerobic methane-oxidizing bacteria are Candidatus methylomirabilis; the major anammox bacterium is Candidatus Brocadia.
8. The granular sludge as set forth in claim 6, characterized in that the final nitrate consumption rate of the granular sludge is 50-150 mgN/L/d, the ammonia nitrogen consumption rate is 200-1000 mgN/L/d, and the denitrification load is 500-2000 mgN/L/d.
9. The granular sludge as set forth in claim 6, characterized in that the concentration of the granular sludge in the reactor is 5-50 gVSS/L.
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| CN115259378A (en) * | 2022-08-25 | 2022-11-01 | 华南农业大学 | Method for enriching ammonia oxidizing archaea in sewage treatment system |
| CN116354500A (en) * | 2023-04-28 | 2023-06-30 | 北京工业大学 | Device and method for rapidly forming denitrification anaerobic methane oxidation and anaerobic ammonia oxidation symbiotic microorganism |
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