CN1114050A - Low-light-level cellular micro beam instrument - Google Patents
Low-light-level cellular micro beam instrument Download PDFInfo
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- CN1114050A CN1114050A CN 94111760 CN94111760A CN1114050A CN 1114050 A CN1114050 A CN 1114050A CN 94111760 CN94111760 CN 94111760 CN 94111760 A CN94111760 A CN 94111760A CN 1114050 A CN1114050 A CN 1114050A
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Abstract
The present invention features that the pulse Q tuning laser and continuous laser are installed simultaneously in the instrument, at laser irradiated optical path the collimated beam extender lens is installed, at the observing optical path, the interference optical filter and differentiation board are installed, in the resonance cavity of the laser the colour core YAG tuning Q crystal is installed, there are two functions of biosample microprocessing and optical clamp operation. The advantages are small thermal damage, good observing result, simple structure, stable, and capable of measuring the geometric size and moving speed of the biosample.
Description
The present invention relates to the instrument that a kind of bioengineering is used, specifically a kind of laser cell microbeam instrument belongs to the laser biology technical field.
Existing laser cell microbeam instrument is mainly by pulsed laser, He-Ne(helium-neon) formation such as laser instrument, microscope, optical match element, video camera.Existing laser cell microbeam instrument still has following weak point: 1, because the pulsed laser that is used for micro-manufactured only is housed, this kind instrument only has the function of biological sample (as cell, chromosome) being carried out micro-manufactured (as punching, cutting), and use is restricted.2, in micro-illumination path, the Gaussian beam of utilizing matched lenses that laser instrument is sent is converted to the centre of transition and girdles the waist, and focuses on the method that obtains little optically focused spot by microscope then, and design is complicated, to the stability and the coherence request height of laser instrument.3, make the indication light source of laser irradiation with He-Ne laser, make and adjust and indication light path complexity.4, the parasitic light of laser is more in the observation optical path, and observing effect is bad.5, make the Q switched element of laser instrument with dyestuff or electro-optic crystal, the complex structure of laser instrument, poor stability, pulse broad, laser during to biological sample irradiation fire damage big, therefore, certain defective is arranged.Some reports with the laser treatment cell have appearred recently, Jap.P. JP03-297385 and JP63-59875, disclose the Laser Processing cell and utilized the method for laser beam fused cell, the accommodation of its work is comparatively single, can not be used for (animal, plant, microorganism) transgenosis simultaneously, chromosome cutting, Fusion of Cells, also there are certain deficiency in the seizure of biological sample (as cell, chromosome etc.), extraction, separation etc.
The object of the present invention is to provide a kind of novel laser cell microbeam instrument, overcome the single deficiency of work of prior art, make it to have simultaneously and can carry out micro-manufactured (as cell punching, chromosome cutting) and biological sample be carried out micromanipulation (as the seizure of cell, separate, extraction) two big functions, enlarge its range of application biological sample; The objective of the invention is to improve the matching process of laser beam to the micro objective transmission, simplified design is simplified the indication light path, reduces parasitic light, improves observing effect; The present invention also aims to reduce the fire damage of laser, simplify the structure of laser instrument, improve the stability of laser instrument biological sample.
To achieve these goals, the present invention has taked following method:
This laser cell microbeam instrument is by laser instrument, optical match element, microscope, video camera, camera are formed, it is characterized in that in instrument, being equipped with simultaneously continuous wave laser (1) and pulsed laser (2), continuous wave laser (1) is used for the light pincers, pulsed laser (2) is used for that biological sample (as cell, chromosome) is carried out micro-manufactured (punching, cutting etc.) so this instrument and has micro-manufactured and light pincers operation two big functions, has enlarged range of application.
In the laser irradiation light path of continuous wave laser (1) and pulsed laser (2), be provided with collimator and extender lens (7), the Gaussian beam of laser instrument (1) or (2) output is transformed into parallel beam, handle by geometrical optics, simplified design, reduced requirement to laser instrument, in observation optical path, be provided with interference filter (14) and reduced the parasitic light that irradiation laser produces in observation optical path, improved observing effect, in observation optical path, be provided with simultaneously graticule (19), graticule (19) be used to indicate laser after object lens (11) focus on the position and can be used for the physical dimension and the movement velocity of biological sample are measured, adopt graticule (19) to simplify the indication light path.
In the resonator cavity of pulsed laser (2), be provided with colour center YAG crystal (2-3) and make Q switched element, simplified the structure of laser instrument, improved reliability.
Continuous wave laser (1) and pulsed laser (2), available krypton lamp (1-6) is powered by continuous impulse double-purpose electric source (27).
The present invention has following advantage compared with the prior art:
The present invention is owing to be equipped with continuous wave laser (1) and pulsed laser (2) simultaneously, make it to have and to carry out micro-manufactured and light such as biological sample is caught, extracts, separated pincers operation two big functions to biological sample (as cell, chromosome), handle by geometrical optics after in the irradiation light path, having adopted laser that the collimator and extender lens send laser instrument (1) or (2), collimator and extender, simplified design, reduced requirement laser instrument.Colour center YAG crystal with transfer the Q dyestuff to compare with electro-optic crystal, advantage such as it is narrow to have a Q impulse, simple and reliable.Colour center YAG crystal is placed in the resonator cavity of laser instrument (2) and make Q switched element, thereby reduced the fire damage that laser causes biological sample irradiation, and simplified the structure of laser instrument, increased reliability.In observation optical path, be provided with interference filter and graticule, reduced parasitic light, improved observing effect, simplified the indication light path, and can be used for measuring the physical dimension and the movement velocity of biological sample.
Description of drawings:
Fig. 1, laser cell microbeam instrument structural representation.
Fig. 2, another example structure synoptic diagram of laser cell microbeam instrument.
Fig. 3, continuously, the dual-purpose Laser Power Devices of pulsed laser.
Among the figure, 1, continuous wave laser (1-1 full anti-film, 1-2 aperture, 1-3YAG crystal, the 1-4 polarizer, the 1-5 output film, 1-6 krypton lamp, 1-7 uninterruptable power) 2, pulsed laser (2-1 full anti-film, the 2-2 aperture, 2-3 colour center YAG adjusting Q crystal, 2-4YAG crystal, the 2-5 polarizer, the 2-6 output film, the 2-7 xenon lamp, the 2-8 pulse laser power supply) 3, frequency-doubling crystal, 4,45 ° of interference filters, 5, frequency-doubling crystal, 6, interference filter, 7, the collimator and extender lens, 8, neutral filter, 9,45 ° of interference filters, 10, micro objective, 11, sample, 12, microscope illumination system, 13, laser energy, power monitor, 14, interference filter, 15, prism, 16, interference filter, 17, matched lenses, 18, camera, 19, graticule, 20, matched lenses, 21, video camera, 22, video recorder, 23, monitor, 24, lens, 25, the microscope lens body, 26, laser instrument, 27, continuously, the pulse double-purpose Laser Power Devices, 28, high voltage charging power supply, 29, storage capacitor, 30, controllable silicon, 31, diode, 32, inductance, 33, contactor, 34, stabilized current supply, 35, diode, 36, contactor, 37, the high pressure simmering circuit, 38, diode.
Following the present invention embodiment in conjunction with the accompanying drawings is further described:
As shown in Figure 1, continuous wave laser (1) is continuous YAG(yttrium aluminum garnet) laser instrument, (1-1 full anti-film, the 1-2 aperture, the 1-3YAG crystal, the 1-4 polarizer, 1-5 output film, the 1-6 krypton lamp, the 1-7 continuous laser power supply) laser that sends is through frequency-doubling crystal (3), 45 ° of interference filters (4,6), collimator and extender lens (7), neutral colour filter (8) is mapped to 45 ° of interference filters (9), and interference filter (9) places in the microscope body (25).By interfering filter disc (9) laser light reflected to focus on the sample (11) through micro objective (10), the gradient force that utilizes this laser beam to produce on biological sample is realized light pincers operations such as the seizure, extraction, separation to biological sample (as cell, chromosome).Pulsed laser (2) is the pulsed YAG Q-switched laser, (2-1 full anti-film, the 2-2 aperture, 2-3 colour center YAG adjusting Q crystal, the 2-4YAG crystal, the 2-5 polarizer, the 2-6 output film, the 2-7 pulse xenon lamp, the 2-8 pulse power) through frequency-doubling crystal (5), 45 ° of interference filters (6), collimator and extender lens (7), neutral colour filter (8), interference filter (9) and object lens (10) focus on biological sample (11), realize micro-manufactured such as biological sample punching, cutting (as cell punching, Fusion of Cells, chromosome cutting).The present invention makes it to have and can carry out micro-manufactured and light such as biological sample is caught, extracts, separated pincers operation two big functions to biological sample owing to simultaneously continuous wave laser (1) and pulsed laser (2) are housed.The present invention has adopted collimator and extender lens (7) in the irradiation light path, with the laser that laser instrument (1) or laser instrument (2) send, handle by geometrical optics behind the collimator and extender, has simplified design, has reduced the requirement to laser instrument.Colour center YAG crystal is compared with electro-optic crystal with accent Q dyestuff, it is narrow to have Q impulse, advantage such as simple and reliable, the present invention places colour center YAG crystal 2-3 in the resonator cavity of laser instrument (2) and makes Q switched element, thereby reduced the fire damage that laser causes biological sample irradiation, and simplified the structure of laser instrument, increased reliability.The picture of biological sample is through 45 ° of interference filters (9), interference filter (14), prism (15) is to microscopical observation eyepiece or through prism (15), 45 ° of interference filters (16), lens (17) are to camera (18), and camera (18) is in order to photograph required image.The picture of biological sample also can be through interference filter (16), graticule (19), and lens (20) are to video camera (21), and the image that video camera obtains is delivered to video recorder (22) and monitor (23).The present invention is provided with interference filter (14) in observation optical path, its reflection wavelength is the same with incident wavelength, reduced parasitic light, improved observing effect, in observation optical path, be provided with graticule (19), in order to the light spot position of indication laser irradiation on biological sample, simplified the indication light path behind the accepted scale, and can be used for measuring the physical dimension and the movement velocity of biological sample.Interference filter (14) and (16) all place in the microscope body (25), and laser energy, power monitor (13) are used to monitor the energy and the power of laser.
Fig. 2 is the another kind of structure of continuous wave laser (1) and pulsed laser (2) and corresponding to microscopical coupling scheme, laser instrument (26) can be realized continuously or pulse output, as another embodiment, as shown in Figure 2, continuous wave laser among Fig. 1 (1) and pulsed laser (2), the structure of available laser instrument (26) shown in Figure 2, promptly laser instrument (26) can be realized the function of continuous wave laser (1) and pulsed laser (2).Among the figure, 2-1 is a full anti-film, the 2-2 aperture, 2-3 colour center YAG crystal, the 2-4YAG crystal, the 2-5 polarizer, 2-6 output film, 1-6 is a krypton lamp, (27) be continuous, pulse double-purpose Laser Power Devices, krypton lamp 1-6 is powered with power supply by continuous, pulse double-purpose, by the continuous or pulsed operation of krypton lamp 1-6, laser instrument (26) is correspondingly exported continuously or pulse laser, has realized two kinds of functions of laser instrument (1) and laser instrument (2).Continuously, the pulse double-purpose electric source structure as shown in Figure 3, (28) are high voltage charging power supply, it charges to storage capacitor (29).When need laser instrument (26) when making pulsed operation, contactor (33) closed (contactor this moment (36) disconnects), trigger controllable silicon (30), storage capacitor (29) is by krypton lamp 1-6 discharge, pulse laser of laser instrument (26) output, when need laser instrument (26) when exporting continuously, contactor (36) closed (contactor this moment (33) disconnects), laser instrument (26) output continuous laser.When laser instrument (26) needed the output continuous laser, 2-3 shifted out outside the laserresonator with the colour center adjusting Q crystal.(37) are the high pressure simmering circuit among Fig. 3, as krypton lamp pre-burning.The laser of laser instrument (26) output is through collimator and extender lens (7), and neutral filter (8) is coupled to micro objective (10), finishes micro-manufactured and light pincers operation two big functions.Adopt laser instrument (26) shown in Figure 2, simplify the structure, reduced cost.
Claims (4)
1, a kind of laser cell microbeam instrument, by laser instrument, the optical match element, microscope, video camera, camera is formed, and it is characterized in that, continuous wave laser (1) and pulsed laser (2) are installed in instrument simultaneously.
2, microbeam instrument according to claim 1, it is characterized in that, in the laser irradiation light path of continuous laser (1) and pulsed laser (2), be provided with collimator and extender lens (7), in observation optical path, be provided with interference filter (14) and graticule (19).
3, microbeam instrument according to claim 1 is characterized in that, is provided with colour center YAG crystal (2-3) in the resonator cavity of pulsed laser (2).
4, microbeam instrument according to claim 1 is characterized in that, continuous wave laser (1) and the available krypton lamp of pulsed laser (2) (1-6) are powered by continuous impulse double-purpose electric source (27).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 94111760 CN1114050A (en) | 1994-05-17 | 1994-05-17 | Low-light-level cellular micro beam instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 94111760 CN1114050A (en) | 1994-05-17 | 1994-05-17 | Low-light-level cellular micro beam instrument |
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| Publication Number | Publication Date |
|---|---|
| CN1114050A true CN1114050A (en) | 1995-12-27 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 94111760 Pending CN1114050A (en) | 1994-05-17 | 1994-05-17 | Low-light-level cellular micro beam instrument |
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| CN (1) | CN1114050A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1842724B (en) * | 2003-08-25 | 2010-04-28 | 皇家飞利浦电子股份有限公司 | Transmit apodization control for microbeamformers |
-
1994
- 1994-05-17 CN CN 94111760 patent/CN1114050A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1842724B (en) * | 2003-08-25 | 2010-04-28 | 皇家飞利浦电子股份有限公司 | Transmit apodization control for microbeamformers |
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