CN111392842A - Method for recycling water recovered by concentrating and condensing yeast extract enzymatic hydrolysate - Google Patents
Method for recycling water recovered by concentrating and condensing yeast extract enzymatic hydrolysate Download PDFInfo
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Abstract
The invention relates to the technical field of yeast extract production processes, in particular to a method for recycling water by concentrating and condensing enzymolysis liquid of yeast extract. The method comprises the following steps: carrying out rotary evaporation on the yeast extract enzymatic hydrolysate, collecting the yeast extract enzymatic hydrolysate, concentrating and condensing to recover water; concentrating and condensing the purified water and the obtained yeast extract enzymatic hydrolysate to recover water according to the weight ratio of (0.5-1.5): 1, and adjusting the pH value of mixed water to 5-6.5; dissolving the dry yeast by the obtained mixed water after the pH value is adjusted, then producing the yeast extract, and collecting the yeast extract enzymatic hydrolysate for detection. The indexes of the water recovered by the concentration and condensation of the yeast extract enzymatic hydrolysate collected in the research are not obviously different from those of purified water, and the yeast extract enzymatic hydrolysate can be used as a recycled water resource. The invention can reduce the production cost of the yeast extract on one hand, and can reduce sewage discharge, relieve the sewage treatment pressure and protect the environment on the other hand.
Description
Technical Field
The invention relates to the technical field of yeast extract production processes, in particular to a method for recycling water by concentrating and condensing enzymolysis liquid of yeast extract.
Background
The Yeast Extract (YE) is a natural seasoning raw material prepared by taking edible baker's Yeast or beer Yeast as a raw material, performing natural enzymolysis on the raw material, and then performing processes such as concentration, blending, drying and the like, contains nutrient substances such as polypeptide, amino acid, vitamin, trace elements and the like, can increase the nutrition of food, can also strengthen the flavor of the food and cover the peculiar smell of the food, and is a natural, pollution-free, delicious and mellow food ingredient.
The production method of the yeast extract mainly comprises an acidolysis method, an enzymolysis method and an autolysis method, wherein the acidolysis method is to decompose yeast cells by using an acidic substance, and the obtained extract has high yield but large production pollution; the extract obtained by the enzymolysis method has good quality, but high cost, and is not commonly used at home at present; the autolysis method has high decomposition efficiency, and the obtained extract has good quality and low cost, and is mostly used in China at present.
The yeast extract can generate a large amount of yeast organic wastewater in the preparation process, and the sources of the wastewater mainly comprise enzymolysis liquid concentrated condensed reclaimed water, equipment cleaning water, workshop floor cleaning water, steam condensate water and the like. At present, little research is done on wastewater in yeast extract production, and almost no condensed water in yeast extract enzymatic hydrolysate concentration exists, but in a recycling system for yeast extract production drainage provided in the patent CN209386273U, the related water resource is steam condensed water used in yeast extract production, but the system is not suitable for recycling the condensed water in yeast extract concentration.
Disclosure of Invention
In view of the above, the present invention provides a method for recycling water recovered by concentrating and condensing the enzymatic hydrolysate of yeast extract. The method can not only ensure the product quality, but also reduce the waste water discharge, reduce the production cost and achieve the purposes of energy conservation and emission reduction.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a method for recycling yeast extract enzymatic hydrolysate after concentration and condensation, which comprises the following steps:
step (1): carrying out rotary evaporation on the yeast extract enzymatic hydrolysate, collecting the yeast extract enzymatic hydrolysate, concentrating and condensing to recover water;
step (2): concentrating and condensing the purified water and the obtained yeast extract enzymatic hydrolysate to recover water according to the weight ratio of (0.5-1.5): 1, and adjusting the pH value of mixed water to 5-6.5;
and (3): dissolving dry yeast with mixed water after pH value adjustment, then producing yeast extract, and collecting yeast extract enzymatic hydrolysate.
Preferably, in the step (2), the purified water and the obtained yeast extract enzymatic hydrolysate are condensed and reclaimed water according to the ratio of 0.5: 1 by weight ratio.
Preferably, in the step (2), the pH value of the mixed water is adjusted to 6-6.5.
Preferably, in the step (1), the temperature of the rotary evaporation is 60-70 ℃, and the vacuum degree is 0.085-0.1 MPa.
In the specific embodiment provided by the invention, in the step (1), the temperature of the rotary evaporation is 60 ℃, and the vacuum degree is 0.09 MPa.
Preferably, the number of times of recycling the yeast extract enzymolysis liquid recycled water is 1-3 times.
Preferably, the reagent for adjusting the pH value of the mixed water is an alkaline solution.
Preferably, the alkaline solution is a baking soda solution.
Preferably, the preparation method of the yeast extract enzymatic hydrolysate comprises the following steps: the active dry yeast is obtained by the steps of adding water for activation, dissolving in salt, carrying out enzymolysis and inactivating enzyme.
Preferably, the preparation method of the yeast extract enzymatic hydrolysate comprises the following steps: preparing 20% mass volume percent active yeast solution, adding 4% mass percent salt, mixing uniformly, performing autolysis wall breaking for 6 hours in a 55 ℃ water bath, then adding 0.4% mass percent yeast extraction enzyme, mixing uniformly, performing enzymolysis for 24 hours in a 55 ℃ water bath, and then performing enzyme deactivation for 20 minutes in a 95 ℃ water bath to obtain the yeast extract enzymatic hydrolysate.
The invention provides a method for recycling yeast extract enzymatic hydrolysate after concentration and condensation. The method comprises the following steps: carrying out rotary evaporation on the yeast extract enzymatic hydrolysate, collecting the yeast extract enzymatic hydrolysate, concentrating and condensing to recover water; concentrating and condensing the purified water and the obtained yeast extract enzymatic hydrolysate to recover water according to the weight ratio of (0.5-1.5): 1, and adjusting the pH value of mixed water to 5-6.5; dissolving dry yeast with mixed water after pH value adjustment, then producing yeast extract, and collecting yeast extract enzymatic hydrolysate. The invention has the technical effects that:
1. the indexes of the yeast extract enzymatic hydrolysate concentrated and condensed reclaimed water collected in the research are not obviously different from the indexes of purified water, the indexes of the yeast extract produced by utilizing the yeast extract enzymatic hydrolysate concentrated and condensed reclaimed water are not obviously different from the indexes of the yeast extract produced by utilizing the purified water, the amino nitrogen content (in terms of desalination) is 4.2 percent, the amino nitrogen conversion rate is 57.3 percent, and the amino nitrogen can be used as a recycled water resource.
2. The collected yeast extract enzymatic hydrolysate is concentrated and condensed to recover water, which belongs to organic wastewater, and the enzymatic hydrolysate of most production enterprises is directly volatilized during concentration and is not collected; in the research, the enzymolysis liquid is concentrated, condensed and recycled, and the water is recycled, so that the production cost of the yeast extract can be reduced, the sewage discharge can be reduced, the sewage treatment pressure can be relieved, and the environment can be protected.
Drawings
FIG. 1 is a graph showing the effect of the concentration, condensation and recovery of water during storage in the case of the enzymatic hydrolysate in test example 3 of the present invention.
Detailed Description
The invention discloses a method for recycling water recovered by concentrating and condensing yeast extract enzymatic hydrolysate, and the method can be realized by appropriately improving process parameters by taking the contents into account by the technical personnel in the field. It is expressly intended that all such similar substitutes and modifications which would be obvious to one skilled in the art are deemed to be included in the invention. While the methods and applications of this invention have been described in terms of preferred embodiments, it will be apparent to those of ordinary skill in the art that variations and modifications in the methods and applications described herein, as well as other suitable variations and combinations, may be made to implement and use the techniques of this invention without departing from the spirit and scope of the invention.
The invention provides a method for recycling condensed recycled water of yeast extract enzymatic hydrolysate for reducing the emission of the condensed recycled water of the yeast extract enzymatic hydrolysate. Realizes the reutilization of the water recovered by the concentration and condensation of the yeast extract enzymatic hydrolysate.
In order to verify the availability of the water recovered by concentrating and condensing the yeast extract enzymatic hydrolysate, the invention adopts the following experimental scheme:
a method for recycling water recovered by concentrating and condensing yeast extract enzymatic hydrolysate comprises the following steps:
(1) preparing yeast extract enzymatic hydrolysate: the active dry yeast is obtained by the steps of adding water for activation, dissolving in salt, carrying out enzymolysis, inactivating enzyme and the like;
(2) collecting the water recovered by concentrating and condensing the yeast extract enzymatic hydrolysate: collecting the concentrated and condensed enzymolysis liquid in the yeast extract concentration process at a certain temperature by using a vacuum rotary evaporator to recover water;
(3) setting mixed water (purified water and enzymolysis liquid concentration and condensation recycled water) in different proportions;
(4) adjusting different pH values by mixing water;
(5) dissolving the dry yeast by using the mixed water in the step (4), and preparing yeast extract enzymatic hydrolysate according to the method in the step (1);
(6) physical and chemical index detection: collecting the enzymolysis liquid in (1), (2) and (5), and detecting each physical and chemical index.
(7) Repeating the operations to ensure that the recycling times of the concentrated and condensed enzymolysis liquid recycled water reach 3 times;
(8) the storage test of the concentrated and condensed reclaimed water of the enzymolysis liquid: and (3) collecting the concentrated condensed recycled water in the step (2), standing for a plurality of days at normal temperature under the condition of heat preservation, and observing whether the recycled water is clear and transparent and whether the index reaches the standard.
In the step (2), the temperature of the rotary evaporator is 60-70 ℃, and the vacuum degree is 0.085-0.1 MPa;
in the step (3), the ratio of the purified water to the concentrated and condensed reclaimed water of the enzymolysis liquid is 0.5: 1. 1: 1; 1.5: 1
In the step (4), the pH (5-5.5, 5.5-6, 6-6.5) of the mixed water in the step (3) is changed;
in the steps (1), (2), (5) and (7), the detection indexes are indexes such as amino nitrogen, total nitrogen, protein and the like;
in the step (8), the storage conditions are set as follows: heating at 37 deg.C and 23 deg.C at 90 deg.C for 15min, and storing at room temperature.
Further, the pH adjusting agent is a sodium bicarbonate solution with a certain concentration.
The invention has the following effects: the method provided by the invention can ensure the product quality, reduce the waste water discharge, reduce the production cost and realize energy conservation and emission reduction.
Reagents or instruments used in the method for concentrating, condensing and recycling the yeast extract enzymatic hydrolysate for recycling are all available in the market.
The invention is further illustrated by the following examples:
example 1
(1) Preparing yeast extract enzymatic hydrolysate: preparing 20% active yeast solution (m/v), adding 4% salt (m/m), mixing, autolyzing and wall breaking for 6h in a 55 ℃ water bath, adding 0.4% yeast extraction enzyme (m/m), mixing, performing enzymolysis for 24h in a 55 ℃ water bath, and inactivating enzyme in a 95 ℃ water bath for 20min to obtain yeast extract enzymatic hydrolysate;
(2) collecting the water recovered by concentrating and condensing the yeast extract enzymatic hydrolysate: collecting yeast extract enzymatic hydrolysate at 60 deg.C under 0.09MPa, concentrating, and condensing to recover water;
(3) mixing the purified water with the concentrated and condensed reclaimed water of the enzymolysis liquid, wherein the mixing ratio is 0.5: 1;
(4) adjusting the pH value of the mixed water in the step (3) by using a 3% sodium bicarbonate solution (m/v), and adjusting the pH value to 5-5.5 (original pH);
(5) dissolving the dry yeast by using the mixed water in the step (4), and producing yeast extract enzymatic hydrolysate according to the method in the step (1);
(6) physical and chemical index detection: collecting the enzymolysis liquid in (1) and (5), and detecting various physical and chemical indexes of the enzymolysis liquid.
Example 2
(1) Preparing yeast extract enzymatic hydrolysate: the active dry yeast is obtained by the steps of adding water for activation, dissolving in salt, carrying out enzymolysis, inactivating enzyme and the like (the same as the embodiment 1);
(2) collecting the water recovered by concentrating and condensing the yeast extract enzymatic hydrolysate: collecting yeast extract enzymatic hydrolysate at 60 ℃ and 0.09MPa vacuum degree by using a vacuum rotary evaporator, concentrating, condensing and recovering water;
(3) mixing the purified water with the concentrated and condensed reclaimed water of the enzymolysis liquid, wherein the mixing ratio is 1: 1;
(4) adjusting the pH value of the mixed water in the step (3) by using a 3% sodium bicarbonate solution (m/v), and adjusting the pH value to 5-5.5 (original pH);
(5) dissolving the dry yeast by using the mixed water in the step (4), and producing yeast extract enzymatic hydrolysate according to the method in the step (1);
(6) physical and chemical index detection: collecting the enzymolysis liquid in (1) and (5), and detecting various physical and chemical indexes of the enzymolysis liquid.
Example 3
(1) Preparing yeast extract enzymatic hydrolysate: the active dry yeast is obtained by the steps of adding water for activation, dissolving in salt, carrying out enzymolysis, inactivating enzyme and the like (the same as the embodiment 1);
(2) collecting the water recovered by concentrating and condensing the yeast extract enzymatic hydrolysate: collecting yeast extract enzymatic hydrolysate at 60 ℃ and 0.09MPa vacuum degree by using a vacuum rotary evaporator, concentrating, condensing and recovering water;
(3) mixing the purified water with the concentrated and condensed reclaimed water of the enzymolysis liquid, wherein the mixing ratio is 1.5: 1;
(4) adjusting the pH value of the mixed water in the step (3) by using a 3% sodium bicarbonate solution (m/v), and adjusting the pH value to 5-5.5 (original pH);
(5) dissolving the dry yeast by using the mixed water in the step (4), and producing yeast extract enzymatic hydrolysate according to the method in the step (1);
(6) physical and chemical index detection: collecting the enzymolysis liquid in (1) and (5), and detecting various physical and chemical indexes of the enzymolysis liquid.
Example 4
(1) Preparing yeast extract enzymatic hydrolysate: the active dry yeast is obtained by the steps of adding water for activation, dissolving in salt, carrying out enzymolysis, inactivating enzyme and the like (the same as the embodiment 1);
(2) collecting the water recovered by concentrating and condensing the yeast extract enzymatic hydrolysate: collecting yeast extract enzymatic hydrolysate at 60 ℃ and 0.09MPa vacuum degree by using a vacuum rotary evaporator, concentrating, condensing and recovering water;
(3) mixing the purified water with the concentrated and condensed reclaimed water of the enzymolysis liquid, wherein the mixing ratio is 0.5: 1;
(4) adjusting the pH value of the mixed water in the step (3) by using a 3% sodium bicarbonate solution (m/v), wherein the pH value is adjusted to be 5.5-6;
(5) dissolving the dry yeast by using the mixed water in the step (4), and producing yeast extract enzymatic hydrolysate according to the method in the step (1);
(6) physical and chemical index detection: collecting the enzymolysis liquid in (1) and (5), and detecting various physical and chemical indexes of the enzymolysis liquid.
Example 5
(1) Preparing yeast extract enzymatic hydrolysate: the active dry yeast is obtained by the steps of adding water for activation, dissolving in salt, carrying out enzymolysis, inactivating enzyme and the like (the same as the embodiment 1);
(2) collecting the water recovered by concentrating and condensing the yeast extract enzymatic hydrolysate: collecting yeast extract enzymatic hydrolysate at 60 ℃ and 0.09MPa vacuum degree by using a vacuum rotary evaporator, concentrating, condensing and recovering water;
(3) mixing the purified water with the concentrated and condensed reclaimed water of the enzymolysis liquid, wherein the mixing ratio is 0.5: 1;
(4) adjusting the pH value of the mixed water in the step (3) by using a 3% sodium bicarbonate solution (m/v), wherein the pH value is adjusted to 6-6.5;
(5) dissolving the dry yeast by using the mixed water in the step (4), and producing yeast extract enzymatic hydrolysate according to the method in the step (1);
(6) physical and chemical index detection: collecting the enzymolysis liquid in (1) and (5), and detecting various physical and chemical indexes of the enzymolysis liquid.
Comparative example 1
(1) Preparing yeast extract enzymatic hydrolysate: the active dry yeast is obtained by the steps of adding water for activation, dissolving in salt, carrying out enzymolysis, inactivating enzyme and the like (the same as the embodiment 1);
(2) collecting the water recovered by concentrating and condensing the yeast extract enzymatic hydrolysate: collecting yeast extract enzymatic hydrolysate at 60 deg.C under 0.09MPa, concentrating, and condensing to recover water;
(3) concentrating and condensing the enzymatic hydrolysate in the step (2) to recover water to dissolve the dry yeast, and producing the enzymatic hydrolysate of the yeast extract according to the process in the step (1);
(4) physical and chemical index detection: and (3) collecting the samples in the steps (1), (2) and (3), and detecting various physical and chemical indexes of the samples.
Comparative example 2
(1) Preparing yeast extract enzymatic hydrolysate: the active dry yeast is obtained by the steps of adding water for activation, dissolving in salt, carrying out enzymolysis, inactivating enzyme and the like (the same as the embodiment 1);
(2) collecting the water recovered by concentrating and condensing the yeast extract enzymatic hydrolysate: collecting yeast extract enzymatic hydrolysate by using a vacuum rotary evaporator at 60 ℃ under the vacuum degree of 0.09MPa, concentrating and condensing to recover water;
(3) dissolving dry yeast by using purified water, and producing yeast extract enzymatic hydrolysate according to the process in the step (1);
(4) physical and chemical index detection: and (3) collecting the samples in the steps (1), (2) and (3), and detecting various physical and chemical indexes of the samples.
Test example 1 index detection of enzymatic hydrolysate
The indexes of the enzymatic hydrolysate in examples 1 to 5 and comparative examples 1 to 2 were measured, and the results are shown in Table 1.
TABLE 1 indexes of enzymatic hydrolysate of yeast extract at different compounding ratios
| Comparative example | Amino nitrogen (%) | Total nitrogen (%) | Protein (%) | Monosodium glutamate (%) |
| Comparative example 1 | 0.56±0.01 | 1.2±0.01 | 8.1±0.01 | 7.4±0.01 |
| Comparative example 2 | 0.58±0.01 | 1.2±0.01 | 8.1±0.01 | 7.4±0.01 |
| Example 1 | 0.60±0.02 | 1.3±0.01 | 8.2±0.02 | 8.0±0.01 |
| Example 2 | 0.56±0.01 | 1.3±0.02 | 8.1±0.01 | 7.7±0.02 |
| Example 3 | 0.56±0.01 | 1.3±0.01 | 8.1±0.01 | 7.5±0.01 |
| Example 4 | 0.59±0.01 | 1.2±0.02 | 7.5±0.01 | 7.8±0.01 |
| Example 5 | 0.61±0.01 | 1.2±0.01 | 7.5±0.01 | 8.2±0.01 |
In the embodiments 1 to 3, the purified water and the concentrated and condensed reclaimed water of the enzymolysis liquid are compounded according to different proportions and screened to obtain: when water purification: concentrating and condensing the enzymolysis liquid to recover water which is 0.5: 1, the prepared yeast extract has slightly higher amino nitrogen content than other mixed water systems although the difference is not obvious, so that the optimal mixed water is 1 part of pure water, and the enzymolysis liquid is concentrated and condensed to recover 2 parts of water.
In examples 4 to 5, the pH of the mixed water (purified water and water recovered by condensing and condensing the enzymatic hydrolysate) was adjusted to obtain: when the pH range of the mixed water (purified water: water recovered by concentrating and condensing enzymolysis liquid) is 6-6.5, the prepared yeast extract has slightly higher amino nitrogen content than other mixed water systems although the difference is small, so the optimal pH range of the mixed water is 6-6.5.
Test example 2 index detection of enzymatic hydrolysate collected for 3 consecutive times
The physical and chemical indexes of the enzymolysis liquid concentrated and condensed recycled water and the enzymolysis liquid of the reproduced yeast extract collected for 3 times in the example 5 are detected, and the results are shown in tables 2 and 3:
TABLE 2 physical and chemical indexes of concentration, condensation and recovery of water for enzymolysis solution of yeast extract
TABLE 3 physical and chemical indexes of yeast extract enzymatic hydrolysate (wastewater reuse)
From the test results, the continuously collected yeast extract enzymatic hydrolysate concentrated and condensed reclaimed water has the same index as that of pure water, and basically does not contain substances such as amino nitrogen, total nitrogen and the like (see table 2), and the yeast extract enzymatic hydrolysate concentrated and condensed reclaimed water has the possibility of being reused; the yeast extract is produced by continuously utilizing the yeast extract concentrated condensed reclaimed water, the index difference between the obtained yeast extract enzymatic hydrolysate and the yeast extract enzymatic hydrolysate produced by purified water is smaller (see table 3), and the yeast extract enzymatic hydrolysate concentrated condensed reclaimed water can be reused; the yeast extract enzymatic hydrolysate is concentrated, condensed and recycled, and the recycled water and purified water are compounded and utilized to obtain the optimal proportion, namely purified water: concentrating and condensing the yeast extract enzymatic hydrolysate to recover water which is 2: 1 (see table 1), the amino nitrogen of the obtained yeast extract enzymatic hydrolysate is 0.61%, the total nitrogen is 1.2%, the protein is 7.5%, and the sodium glutamate is 8.2%, which is slightly superior to the indexes of the yeast extract enzymatic hydrolysate obtained by water purification production.
Test example 3 storage test of concentrated condensed water of enzymatic hydrolysate
The concentrated condensed reclaimed water in the step (2) of example 5 was collected under the following storage conditions: storing at room temperature (23 deg.C), maintaining at 37 deg.C, heating at 90 deg.C for 15min, standing for several days, and observing whether the recovered water is clear and transparent and the index is up to standard. The results are shown in FIG. 1.
As can be seen from FIG. 1, the water recovered by concentration and condensation of the yeast extract hydrolysate generates white floccule (floccule is shown in the square box) when stored at normal temperature and under the condition of heat preservation, and the liquid smells bad, but the phenomenon does not occur when the water recovered by concentration and condensation of the yeast extract hydrolysate is stored for 15 days after being heated, so that the water recovered by concentration and condensation of the yeast extract hydrolysate needs to be used within 5 hours to ensure the product quality, and the water cannot stay overnight.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (10)
1. A method for recycling water recovered by concentrating and condensing yeast extract enzymatic hydrolysate is characterized by comprising the following steps:
step (1): carrying out rotary evaporation on the yeast extract enzymatic hydrolysate, collecting the yeast extract enzymatic hydrolysate, concentrating and condensing to recover water;
step (2): concentrating and condensing the purified water and the obtained yeast extract enzymatic hydrolysate to recover water according to the weight ratio of (0.5-1.5): 1, and adjusting the pH value of mixed water to 5-6.5;
and (3): dissolving dry yeast with mixed water after pH value adjustment, then producing yeast extract, and collecting yeast extract enzymatic hydrolysate.
2. The method as claimed in claim 1, wherein in the step (2), the purified water and the obtained yeast extract enzymatic hydrolysate are condensed to recover water according to a ratio of 0.5: 1 by weight ratio.
3. The method according to claim 1, wherein in the step (2), the pH value of the mixed water is adjusted to 6-6.5.
4. The method according to claim 1, wherein in the step (1), the temperature of the rotary evaporation is 60-70 ℃ and the vacuum degree is 0.085-0.1 MPa.
5. The method according to claim 4, wherein the degree of vacuum is 0.085 to 0.09 MPa.
6. The method according to claim 1, wherein the number of times of reusing the yeast extract enzymatic hydrolysate recycled water is 1 to 3.
7. The method of claim 1, wherein the agent for adjusting the pH of the mixed water is an alkaline solution.
8. The method according to claim 7, characterized in that the alkaline solution is a baking soda solution.
9. The method according to claim 1, wherein the preparation method of the yeast extract enzymatic hydrolysate is as follows: the active dry yeast is obtained by the steps of adding water for activation, dissolving in salt, carrying out enzymolysis and inactivating enzyme.
10. The method according to claim 9, wherein the yeast extract enzymatic hydrolysate is prepared by: preparing 20% mass volume percent active yeast solution, adding 4% mass percent salt, mixing uniformly, performing autolysis wall breaking for 6 hours in a 55 ℃ water bath, then adding 0.4% mass percent yeast extraction enzyme, mixing uniformly, performing enzymolysis for 24 hours in a 55 ℃ water bath, and then performing enzyme deactivation for 20 minutes in a 95 ℃ water bath to obtain the yeast extract enzymatic hydrolysate.
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