CN111876347B - Salt-tolerant bacillus licheniformis A-A2-10, application and application method thereof - Google Patents
Salt-tolerant bacillus licheniformis A-A2-10, application and application method thereof Download PDFInfo
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Abstract
The invention belongs to the technical field of microbial treatment of wastewater, and particularly relates to a salt-tolerant bacterium strain and salt-tolerant bacillus licheniformisBacillus licheniformis The application of A-A2-10 in producing polyglutamic acid and recovering ammonium sulfate from glutamic acid production wastewater. Salt-tolerant bacillus licheniformisBacillus licheniformis A-A2-10, the strain is cultured in China Center for Type Culture Collection (CCTCC) in 6-3-2020, with the preservation number of CCTCC NO: m2020051. Bacillus licheniformisBacillus licheniformis The invention provides an application of A-A2-10 in synthesizing polyglutamic acid by utilizing glutamic acid residual in glutamic acid wastewater under a high-salt environment and degrading residual sugar in the wastewater, which is the content to be protected in particular. The strain used by the invention not only can degrade residual sugar which is difficult to utilize in a high-salt environment, but also can utilize residual glutamic acid to synthesize polyglutamic acid; the strain and the treatment process can reduce the residual sugar to be below 0.2 percent, realize the direct concentration, drying and recovery of ammonium sulfate from the waste liquid, and solve the problems of high residual sugar and incapability of concentration and drying of the traditional glutamic acid mother liquor.
Description
Technical Field
The invention belongs to the technical field of microbial treatment of wastewater, and particularly relates to a salt-tolerant bacillus licheniformisBacillus licheniformisA-A2-10, and the salt-tolerant Bacillus licheniformisBacillus licheniformisThe application of the A-A2-10 strain in the production of polyglutamic acid and the recovery of ammonium sulfate in the wastewater from glutamic acid production, and the salt-tolerant Bacillus licheniformisBacillus licheniformisA-A2-10 application method.
Background
Monosodium glutamate is a flavoring, and the main component of monosodium glutamate is sodium glutamate. The monosodium glutamate can improve the delicate flavor of food, is widely used in Chinese dishes, and is also a large country for monosodium glutamate production in China.
A large amount of waste water is generated in the production process of monosodium glutamate, the production waste water is high-salinity and high-concentration organic waste water, the components are complex, the treatment difficulty is high, the daily discharge causes great harm to the environment and water sources, wherein ammonium sulfate is the main component in the waste water, but the concentration of mother liquor and the recovery of salt are greatly limited because residual sugar is difficult to degrade. With the development of fermentation industry, the waste liquid generated in glutamic acid production is more and more, and becomes one of the main factors for limiting production, so that the method has great significance for treating and recycling the production waste water.
The treatment of the glutamic acid production wastewater at the present stage mainly comprises microbial treatment, physical adsorption treatment and the like. The disadvantages of various ways are gradually exposed during long-term use: 1. the physiological activities of the microorganisms are influenced by the acidic living environment and cannot be normally carried out, so that the processing capacity is influenced. 2. When physical adsorption is carried out, a large amount of adsorbents including active carbon, adsorption resin and the like need to be added, and the cost is high.
CN105132323A discloses a salt-tolerant bacillus and its application in the treatment of high-salt wastewater, wherein the high-salt wastewater disclosed in the patent document refers to wastewater with high salt content in general, and a wastewater sample is from the wastewater of a coal chemical plant in Jilin city, which is seen to have high salt content but not contain residual sugar. However, the monosodium glutamate wastewater not only has high salt content, such as ammonium sulfate, but also has high organic matter content, and the wastewater also contains a large amount of residual sugar.
The method disclosed in the above-mentioned document has the following limitations:
1) the waste water contains a large amount of residual sugar, so that the waste water cannot be highly concentrated, the yield of the ammonium sulfate recovered by crystallization is limited, a large amount of waste mother liquor is still produced, the waste mother liquor accounts for 1/4-1/5 of the total amount of the original waste liquor, and the residual sugar in the mother liquor is difficult to hydrolyze again, so that the waste liquid becomes high-viscosity paste, the addition amount of the waste liquid in the solid fertilizer is very limited, and the market demand and the circulation of the liquid fertilizer are also greatly limited, so that the fundamental problem of large waste liquor output cannot be solved.
2) Although the concentrated waste liquid can be treated in large quantity by adopting the process of preparing the granulated fertilizer by a spraying method, the main component is ammonium sulfate, and the contained amino acid is greatly damaged in the preparation process, so that the fertilizer efficiency is limited.
3) In addition, more importantly, the spraying process has been gradually eliminated in recent years due to environmental pollution caused by the smoke generated by high-temperature carbonization, so that a new green and environment-friendly mode is urgently needed to upgrade and improve the waste liquid treatment mode so as to overcome the defects.
Disclosure of Invention
In order to solve the technical problem, the invention provides salt-tolerant bacillus licheniformisBacillus licheniformisA-A2-10, the adoption of the strain can obviously reduce the treatment cost of the glutamic acid production wastewater, and overcomes the influence of the acidic living environment on the microorganismThe defect that the physiological activity cannot be normally carried out so as to influence the processing capability is overcome, more than 4 percent of agricultural polyglutamic acid can be obtained, and great economic benefit is generated; in addition, the invention also reduces the residual sugar in the wastewater to be within 0.2 percent, avoids the problem that the residual sugar content is high so that the wastewater cannot be highly concentrated, and fully utilizes the wastewater so that the amount of the treated wastewater is 0.
The Bacillus licheniformis A-A2-10 is preserved in China center for type culture Collection 3 month 6 year 2020, and the preservation number is CCTCC NO: m2020051, deposit address: wuhan university Collection No. 202, Wuhan City, Hubei province, China. The following Bacillus licheniformis A2-10 were all the strains deposited as described above.
The length of the 16S sequence fragment of the strain is 1453bp, and the nucleotide sequence of the strain is shown in a sequence table 1.
The salt-tolerant Bacillus licheniformisBacillus licheniformisThe application of A-A2-10 in synthesizing polyglutamic acid by utilizing the residual glutamic acid in the glutamic acid wastewater under the high-salt environment and simultaneously degrading the residual sugar in the wastewater is also within the protection scope of the invention.
The concentrated water is formed by converging the glutamic acid fermentation of the glutamic acid production wastewater and the subsequent ion exchange and neutralization links of the monosodium glutamate production;
or the glutamic acid production wastewater is the unconcentrated wastewater formed by the confluence of glutamic acid fermentation and subsequent ion exchange and neutralization links of monosodium glutamate production;
preferably, the content of ammonium sulfate in the glutamic acid production wastewater is between 5 and 40 percent.
The salt-tolerant Bacillus licheniformisBacillus licheniformisA-A2-10 strain for treating high-salt glutamic acid waste water, which comprises the following steps:
(1) adjusting the salt content and pH of the high-salt glutamic acid wastewater, and sterilizing;
(2) salt-tolerant bacillus licheniformisBacillus licheniformisInoculating the A-A2-10 strain into the wastewater, culturing in a ventilating way, and adding high-valence salt to settle polyglutamic acid;
(3) and (2) drying the precipitate after solid-liquid separation to obtain polyglutamate, and concentrating and drying clear liquid obtained by solid-liquid separation to obtain crude ammonium sulfate.
Preferably, (1) adjusting the salt content of the high-salt glutamic acid wastewater to be less than or equal to 25 percent;
preferably, (1) adjusting the pH value to 5.5-8.0 by using ammonia water;
preferably, (1) sterilizing at 110-120 ℃;
preferably, (1) sterilizing for 10-20 minutes.
(2) Salt-tolerant bacillus licheniformisBacillus licheniformisInoculating the A-A2-10 strain into the wastewater according to the inoculation amount of 0.5-1.0%;
preferably, the culture is carried out in a ventilation way at the temperature of 30-37 ℃ in the step (2);
preferably, the medium is ventilated and cultured for 48 to 96 hours;
preferably, the aeration culture in the step (2) is stopped until the residual sugar in the wastewater is less than or equal to 0.2 percent;
preferably, in the step (2), 0.05-0.1% of high valence ion sedimentary polyglutamic acid is added into the material after the ventilation culture;
(2) the added high-valent salt is any one of soluble copper salt, calcium salt, zinc salt, iron salt and magnesium salt.
(3) The temperature of the medium drying is 60-90 ℃.
And (3) performing multi-effect evaporation or forced circulation evaporation on the clear liquid obtained by solid-liquid separation, and then preparing ammonium sulfate by a fluidized bed drying method at the drying temperature of 100-120 ℃.
The salt-tolerant Bacillus licheniformisBacillus licheniformisA-A2-10 method for treating high-salt glutamic acid wastewater, comprising the following steps:
(1) adjusting the salt content of the high-salt glutamic acid wastewater to be less than or equal to 25%, adjusting the pH value to be 5.5-8.0 by using ammonia water, and sterilizing at 110-120 ℃ for 10-20 minutes;
(2) bacillus licheniformisBacillus licheniformisA-A2-10 is added into the wastewater according to the inoculation amount of 0.5-1.0%, the aeration culture is carried out for 48-96 hours at the temperature of 30-37 ℃, the culture is stopped when the residual sugar in the wastewater is less than or equal to 0.2%, and high-valence salt is added to the wastewater to settle polyglutamic acidAn acid; the high-valence salt is any one of soluble copper salt, calcium salt, zinc salt, iron salt and magnesium salt;
(3) and (3) performing solid-liquid separation on the precipitate obtained in the step (2), drying at 60-90 ℃ to obtain polyglutamate, performing multi-effect evaporation or forced circulation evaporation on clear liquid obtained by the solid-liquid separation, and preparing ammonium sulfate by a fluidized bed drying method at the drying temperature of 100-120 ℃ to obtain crude ammonium sulfate.
Compared with the scheme in the background technology, the invention polymerizes the glutamic acid in the wastewater into the polyglutamic acid with very high fertilizer efficiency, and the product effect is remarkably improved.
In addition, the solution disclosed in the background art is a common process in the industry at present, but as the environmental protection requirement of the industry is higher and higher, the process is gradually eliminated and even shut down. The invention is improved based on the above big background, the invention can achieve the purposes of no waste water discharge, low energy consumption, 100 percent recovery of ammonium sulfate and generation of a polyglutamic acid byproduct with very high price, and the added value of the waste water can be improved by more than 5 times compared with the prior art, thereby having very good prospect.
The invention has the beneficial effects that:
(1) the strain used by the invention not only can degrade residual sugar which is difficult to utilize in a high-salt environment, but also can utilize residual glutamic acid to synthesize polyglutamic acid;
(2) the strain and the treatment process can reduce the residual sugar to be below 0.2 percent, realize the direct concentration, drying and recovery of ammonium sulfate from the waste liquid, and solve the problems of high residual sugar and incapability of concentration and drying of the traditional glutamic acid mother liquor;
(3) the strain and the treatment process used in the invention have low operation cost, low energy consumption and high value of byproducts, can be continuously reused in industrial production, and obviously improves the economic benefit.
Drawings
FIG. 1 shows Bacillus licheniformis in the present inventionBacillus licheniformis A-A2-10 cell morphology;
FIG. 2 shows Bacillus licheniformis in the present inventionBacillus licheniformis A-A2-10;
FIG. 3 is the crude ammonium sulfate after concentration and drying as described in example 1;
FIG. 4 is the polyglutamic acid iron salt after drying by solid-liquid separation as described in example 3.
Detailed Description
Embodiments of the present invention will be described in detail below with reference to examples, but it will be understood by those skilled in the art that the following examples are only illustrative of the present invention and should not be construed as limiting the scope of the present invention. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.
Example 1
Bacillus licheniformisBacillus licheniformisThe application of A-A2-10 in degrading residual sugar in high-salt glutamic acid wastewater comprises the following steps:
step 1, adjusting the salt content of high-salt glutamic acid wastewater to 38%, adding water to dilute the high-salt glutamic acid wastewater to 23%, adjusting the pH to 6.0, and sterilizing at 115 ℃ for 15 minutes;
step 2, bacillus licheniformisBacillus licheniformis Inoculating A-A2-10 into the wastewater according to the inoculation amount of 0.8%, wherein the culture temperature is 32 ℃, and the aeration culture is carried out for 55 hours, and the residual sugar in the wastewater is up to 0.17%;
step 3, adding 0.08 percent of blue vitriol into the solution in the step 2, and settling the polyglutamic acid;
4, performing solid-liquid separation on the precipitate obtained in the step 3, and drying at a low temperature of 80 ℃ to obtain polyglutamic acid copper salt;
and 5, performing forced circulation evaporation on the clear liquid obtained in the step 3 and the solid-liquid separation, and drying the clear liquid at 100 ℃ by using a fluidized bed to obtain a crude product of ammonium sulfate.
Example 2
Bacillus licheniformisBacillus licheniformisThe application of A-A2-10 in degrading residual sugar in high-salt glutamic acid wastewater comprises the following steps:
step 1, adjusting the salt content of the high-salt glutamic acid wastewater to 21%, adjusting the pH to 7.0, sterilizing at 115 ℃ for 15 minutes;
step (ii) of2. Bacillus licheniformisBacillus licheniformis Inoculating the A-A2-10 into the wastewater according to the inoculation amount of 0.5-1.0%, wherein the culture temperature is 37 ℃, the culture time is 81 hours, and the residual sugar content in the wastewater is up to 0.14%;
step 3, adding 0.06% calcium chloride into the solution obtained in the step 2 to settle the polyglutamic acid;
4, performing solid-liquid separation on the precipitate obtained in the step 3, and drying at a low temperature of 70 ℃ to obtain polyglutamic acid calcium salt;
and 5, performing multi-effect evaporation concentration on the clear liquid obtained in the step 3 and obtained by solid-liquid separation, and drying the clear liquid at 120 ℃ by using a fluidized bed to obtain a crude product of ammonium sulfate.
Example 3
Bacillus licheniformisBacillus licheniformisThe application of A-A2-10 in degrading residual sugar in high-salt glutamic acid wastewater comprises the following steps:
step 1, adjusting the salt content of the high-salt glutamic acid wastewater to 12%, adjusting the pH to 5.5, sterilizing at 115 ℃ for 15 minutes;
step 2, bacillus licheniformisBacillus licheniformis Inoculating A-A2-10 into wastewater according to the inoculation amount of 1.0%, culturing at 35 deg.C under ventilation for 72 hr until the residual sugar content in wastewater reaches 0.11%;
step 3, adding 0.07 percent of ferric sulfate into the solution obtained in the step 2 to settle the polyglutamic acid;
4, performing solid-liquid separation on the precipitate obtained in the step 3, and drying at a low temperature of 65 ℃ to obtain poly (ferric glutamate);
and 5, performing multi-effect evaporation concentration on the clear liquid obtained in the step 3 and the solid-liquid separation solution, and drying the clear liquid at the temperature of 110 ℃ by using a fluidized bed to obtain a crude product of ammonium sulfate.
Example 4
Bacillus licheniformisBacillus licheniformisThe application of A-A2-10 in degrading residual sugar in high-salt glutamic acid wastewater comprises the following steps:
step 1, adjusting the salt content of the high-salt glutamic acid wastewater to 8%, adjusting the pH to 5.5, sterilizing at 115 ℃ for 15 minutes;
step 2, bacillus licheniformisBacillus licheniformis Inoculating A-A2-10 to the wastewater at an inoculum size of 0.7%, and treating at 36 deg.CVentilating and culturing for 60 hours, and reducing the residual sugar in the wastewater to 0.14 percent;
step 3, adding 0.1% magnesium sulfate into the solution obtained in the step 2 to settle the polyglutamic acid;
4, performing solid-liquid separation on the precipitate obtained in the step 3, and drying at a low temperature of 90 ℃ to obtain polyglutamic acid magnesium salt;
and 5, performing forced circulation evaporation on the clear liquid obtained in the step 3 and the solid-liquid separation solution, and drying the clear liquid in a fluidized bed at 100 ℃ to obtain a crude product of ammonium sulfate.
Comparative example 1
The commonly used glutamic acid wastewater treatment process (multi-effect evaporation method for concentrating wastewater and crystallizing and recovering ammonium sulfate) in the industry is taken as a comparative example 1.
Compared with the data in the embodiments 1-4 of the invention, the glutamic acid wastewater treatment process (multi-effect evaporation method for concentrating wastewater and crystallizing and recovering ammonium sulfate) commonly used in the prior industry is as follows:
TABLE 1 comparison of ammonium sulfate yields, etc., of examples 1-1 with comparative example 1
As can be seen from the data in Table 1 above, under the scheme of comparative example 1, the yield of ammonium sulfate is only 50-60%, while the recovery rate of ammonium sulfate in examples 1-4 of the invention reaches more than 91%; compared with the aspect of the yield of ammonium sulfate, the mode of treating the glutamic acid wastewater in the invention is obviously superior to the treatment mode in the comparison document 1;
in comparative example 1, no other high value-added by-products were produced, whereas in the present invention, more than 4% of agricultural polyglutamic acid was produced; in addition, the cost of treating each ton of wastewater in the comparative example 1 is about 150 yuan, while each ton of wastewater can generate 500-600 yuan in the invention, and compared with the economic benefit, the invention brings huge economic benefit and is obviously superior to the treatment mode of the comparative document 1;
the ammonium sulfate content of the obtained product is high by the method, and the product is suitable to be used as a raw material of a granular fertilizer or be used for manufacturing the granular fertilizer.
In the comparative example 1, the amount of the treated wastewater still accounts for 20-25%, and the zero emission of the wastewater can be realized in the invention, which shows that the method is more environment-friendly;
the residual sugar in the wastewater in example 1 is reduced to 0.17%; in example 2, the residual sugar in the wastewater reaches 0.14 percent; the residual sugar in the wastewater in example 3 is reduced to 0.11%; the residual sugar in the wastewater in example 4 is reduced to 0.14%; from the view of residual sugar in the wastewater, the wastewater in the invention is obviously low in residual sugar content after treatment, and the problem that the wastewater cannot be highly concentrated is not caused, which is the reason why the yield of the ammonium sulfate recovered by crystallization is high in the invention, and the problem of large waste liquid output is thoroughly solved by the method in the invention.
And compared with the method of comparative example 1 in the background art, the method comprises the following steps of 4) hydrolysis: the method comprises the following steps of drying feed liquid A, crushing the feed liquid A into powder by a crusher, then placing the powder into a reaction kettle, adding 6-8 mol/of hydrochloric acid to the powder, and taking the raw material as the standard, wherein generally the drying temperature is higher, and the temperature of more than 500 ℃ is generally adopted for direct drying, so that organic matters are seriously carbonized, and only inorganic ammonium sulfate is basically left in the product; the glutamic acid wastewater contains a lot of amino acids including glutamic acid, and various amino acids are seriously damaged at 500 ℃. The invention realizes the crystallization and recovery of ammonium sulfate at low temperature, and changes the glutamic acid into the polyglutamic acid with higher market price. (the commercial price of the glutamic acid is 3000-4000 per ton generally, while the pure product of the polyglutamic acid is about 20 ten thousand per ton), obviously, the economic benefit of the invention is higher.
Example 5
Taking glutamic acid mother liquor discharged after ion exchange extraction of glutamic acid in a monosodium glutamate factory in Shandong, wherein the salt content of the glutamic acid mother liquor is about 25.4%; taking the mother liquor as wastewater to be treated for later use;
example 6
Taking a sample in an original waste mother liquor discharge port 1 of another monosodium glutamate manufacturer in Shandong, and taking the mother liquor as wastewater to be treated for later use;
example 7
Taking the wastewater after the bacteria extraction in the monosodium glutamate production from the manufacturer in the embodiment 6 as the wastewater to be treated for later use;
example 8
Wastewater after monosodium glutamate production treatment is taken from the manufacturer in example 6 and is wastewater to be treated;
example 9
Wastewater of a total discharge port in monosodium glutamate production is taken from a manufacturer in example 6 and is wastewater to be treated;
the wastewater from examples 5-9 was sampled and treated by the method of example 1, and the results were as follows:
TABLE 2 comparison of ammonium sulfate yields in examples 5 to 9
As can be seen from the above tables, the invention also obtains excellent treatment effect by treating the wastewater of monosodium glutamate manufacturers, namely, the recovery rate of ammonium sulfate is high and cannot be over 91 percent, the amount of the treated wastewater is 0, and more than 4 percent of agricultural polyglutamic acid is generated; the economic benefit generated by each ton of wastewater is about 500-600 yuan/ton.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Sequence listing
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cgcgctttac gcccaataat tyccgggaca acgcttgcca cctacgtatt accgcggctg 960
stggcacgta gttagccgtg gctttytggt taggtaccgt craggtaccg ccctattcga 1020
acggtacttg ttyttcccta acaacagagt ttwmsrwtcc gaaaaccttc atcacctcac 1080
gcggcgtksk ysyswsaswy tyktswysmk wgmkkmrkay tscykmctsc kkmswsyckk 1140
rrsmgkttsk smskyrkkts wgysmymsyg tksmskmkcy msmtykywgs ytkgskwsrc 1200
rtwsttgcct tggtgagccg ttacctcccc aactaggtaa tgcgccgcgg gtccatttgt 1260
aagtggtagc taaaagccac cttttataat tgaaccatgc ggttcaatca agcatccggt 1320
attagccccg gtttcccgga gttatcccag tcttacaggc aggttaccca cgtgttactc 1380
acccgtccgc cgctaacatc agggagcaag ctcccatctg tccgctcgac tgcatgtata 1440
gctgcgcccc gcg 1453
Claims (18)
1. Salt-tolerant Bacillus licheniformis (Bacillus licheniformis) A-A2-10, wherein the strain is preserved in China center for type culture Collection 3 month 6 year 2020 with the preservation number of CCTCC NO: m2020051.
2. The use of the salt-tolerant Bacillus licheniformis (Bacillus licheniformis) A-A2-10 according to claim 1 for synthesizing polyglutamic acid by utilizing the residual glutamic acid in glutamic acid wastewater under high-salt environment and simultaneously degrading the residual sugar in the wastewater.
3. The use of the salt-tolerant Bacillus licheniformis (Bacillus licheniformis) A-A2-10 according to claim 1 for producing fertilizer from glutamic acid wastewater in high salt environment.
4. The use of claim 2 or 3, wherein the glutamic acid wastewater is concentrated water obtained by the fermentation of glutamic acid and the confluence of each link of ion exchange and neutralization in the subsequent monosodium glutamate production; or the glutamic acid wastewater is not concentrated wastewater formed by converging glutamic acid fermentation and subsequent ion exchange and neutralization links in monosodium glutamate production.
5. The use according to claim 2 or 3, wherein the content of ammonium sulfate in the glutamic acid wastewater is between 5 and 40 percent.
6. Use according to claim 2 or 3, comprising the steps of:
(1) adjusting the salt content and pH of the high-salt glutamic acid wastewater, and sterilizing;
(2) inoculating salt-tolerant Bacillus licheniformis (Bacillus licheniformis) A-A2-10 strain into wastewater, culturing under ventilation, and adding high-valence salt to precipitate polyglutamic acid; the added high-valence salt is any one of soluble copper salt, calcium salt, zinc salt, iron salt and magnesium salt;
(3) and (2) drying the precipitate after solid-liquid separation to obtain polyglutamate, and concentrating and drying clear liquid obtained by solid-liquid separation to obtain crude ammonium sulfate.
7. The use according to claim 6,
(1) regulating the salt content of the high-salt glutamic acid wastewater to be less than or equal to 25 percent.
8. The use according to claim 6, wherein (1) the pH is adjusted to 5.5 to 8.0 with aqueous ammonia.
9. The use according to claim 6, wherein in (1), sterilization is carried out at 110 to 120 ℃.
10. The use according to claim 6, wherein in (1), the sterilization is carried out for 10 to 20 minutes.
11. The use according to claim 6, wherein (2) the salt-tolerant Bacillus licheniformis A-A2-10 strain is inoculated into the wastewater at an inoculum size of 0.5-1.0%.
12. The use according to claim 6, wherein the cultivation in (2) is carried out with aeration at 30-37 ℃.
13. The use according to claim 6, wherein the cultivation in (2) is carried out under aeration for 48 to 96 hours.
14. The use according to claim 6, wherein the aeration culture in (2) is stopped until the residual sugar in the wastewater is less than or equal to 0.2%.
15. The use according to claim 6, wherein in (2), 0.05-0.1% of high-valent salt sedimentary polyglutamic acid is added into the material after the aeration culture; the added high-valence salt is any one of soluble copper salt, calcium salt, zinc salt, iron salt and magnesium salt.
16. The use according to claim 6, wherein the temperature for drying in (3) is 60-90 ℃.
17. The application of claim 6, wherein the clear liquid obtained by solid-liquid separation is subjected to multi-effect evaporation or forced circulation evaporation, and then the ammonium sulfate is prepared by a fluidized bed drying method, wherein the drying temperature is 100-120 ℃.
18. The use of claim 6, comprising the steps of:
(1) adjusting the salt content of the high-salt glutamic acid wastewater to be less than or equal to 25 percent, adjusting the pH value to be 5.5-8.0 by using ammonia water, and sterilizing for 10-20 minutes at 110-120 ℃;
(2) adding a salt-tolerant Bacillus licheniformis (Bacillus licheniformis) A-A2-10 strain into wastewater according to the inoculation amount of 0.5-1.0%, performing ventilation culture at 30-37 ℃ for 48-96 hours, stopping culture until the residual sugar in the wastewater is less than or equal to 0.2%, and adding high-valent salt to precipitate polyglutamic acid; the high-valence salt is any one of soluble copper salt, calcium salt, zinc salt, iron salt and magnesium salt;
(3) and (3) performing solid-liquid separation on the precipitate obtained in the step (2), drying at 60-90 ℃ to obtain polyglutamate, performing multi-effect evaporation or forced circulation evaporation on clear liquid obtained by the solid-liquid separation, and preparing ammonium sulfate by adopting a fluidized bed drying method at the drying temperature of 100-120 ℃ to obtain crude ammonium sulfate.
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