CN111389478B - Experimental treatment device for tissue slices and application method thereof - Google Patents
Experimental treatment device for tissue slices and application method thereof Download PDFInfo
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- CN111389478B CN111389478B CN202010208341.3A CN202010208341A CN111389478B CN 111389478 B CN111389478 B CN 111389478B CN 202010208341 A CN202010208341 A CN 202010208341A CN 111389478 B CN111389478 B CN 111389478B
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L9/00—Supporting devices; Holding devices
- B01L9/52—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips
- B01L9/523—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips for multisample carriers, e.g. used for microtitration plates
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
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- General Health & Medical Sciences (AREA)
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- Immunology (AREA)
- Pathology (AREA)
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- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses an experimental treatment device for tissue slices, which comprises a box body, wherein the upper end of the box body is connected with a box cover, a plurality of slice frames capable of being placed in a superposition mode are arranged in the box body, and slice frame handles capable of being freely detached can be connected to the slice frames. The invention also discloses a use method of the experimental treatment device for tissue slices, and solves the problem of low efficiency in the prior slicing experiment.
Description
Technical Field
The invention belongs to the technical field of tissue sections, relates to an experimental treatment device for tissue sections, and also relates to a use method of the treatment device.
Background
The existing experiment treatment related to the tissue section mainly comprises operations such as hematoxylin-eosin staining and immunohistochemistry of the tissue section, no corresponding convenient device is available for the treatment of the section in the development process of the experiment, the experiment is usually repeated for a plurality of times in a small amount, and if the experiment is required to be carried out in a large amount, a lot of time is wasted and the efficiency is low.
Disclosure of Invention
The invention aims to provide an experimental treatment device for tissue slices, which solves the problem of low efficiency in the prior slicing experiment.
The invention aims to provide a use method of the experimental treatment device for tissue slices.
The first technical scheme adopted by the invention is that the experimental treatment device for tissue slices comprises a box body, wherein the upper end of the box body is connected with a box cover, a plurality of slice frames capable of being placed in a superposition mode are arranged in the box body, and slice frame handles capable of being freely detached can be connected to the slice frames.
The first technical solution of the invention is also characterized in that,
two ends of the box body are respectively provided with a box body handle.
One end of the box body is hinged with one end of the box cover, a clamping block is arranged at the center of the other end of the box body, a clamping groove is formed in the center of the other end of the box cover, and the clamping block is just clamped at the clamping groove, so that the box body and the box cover are closed.
The slicing frame comprises a frame body, wherein a plurality of slicing placing grooves are regularly divided on the inner side of the frame body, positioning protrusions are respectively arranged at the upper ends of four vertexes of the frame body, positioning grooves are respectively arranged at the lower ends of the four vertexes of the frame body, and the positioning protrusions and the positioning grooves are correspondingly arranged on the frame body up and down; the lower ends of the four vertexes of the frame body are respectively provided with an L-shaped cushion block which is arranged at the periphery of the positioning groove;
a connecting port is arranged at the center of one side of the frame body, and guide sleeves are respectively arranged at the opposite sides of the connecting port.
When a plurality of slice frames are stacked for use, four positioning protrusions on the slice frame of the upper layer are matched with four positioning grooves on the slice frame of the adjacent lower layer.
The sheet frame handle comprises a hollow handle frame A, one end of the handle frame A is connected with a hollow handle frame B, and the handle frame A and the handle frame B are connected to form an L-shaped structure; the opposite sides of the middle part of the handle frame A are respectively provided with a guiding-out hole A, and the opposite sides of one end of the handle frame B away from the handle frame A are respectively provided with a guiding-out hole B;
two brackets A are arranged in the handle frame A in parallel, one ends of the two brackets A are respectively connected with one ends of two brackets B, the two brackets B are arranged in parallel, the brackets B are positioned in the handle frame B, the other ends of the two brackets B are respectively connected with one ends of guide posts, and the other ends of the two guide posts respectively penetrate out of the two guide holes B and are coaxially connected with two guide sleeves at the connecting port;
the middle parts of the two brackets A are respectively provided with movable columns in opposite directions, a spring is arranged between the two movable columns, and the two movable columns respectively penetrate out of the handle frame A from the two guide-out holes A.
The slice placing groove comprises a groove body, one end of the groove body is provided with a guide opening, the opposite sides of the guide opening are respectively provided with a slot, and a slice lifting handle is connected in the slots.
The slice handle comprises a slice bearing plate, one end of the slice bearing plate is horizontally placed in the groove body, the other end of the slice bearing plate is connected with a vertically arranged plugboard, and the plugboard and the slice bearing plate are in an L-shaped structure after being connected;
the center of the outer side of the plugboard is provided with a protruding block;
the width of the slice bearing plate is equal to the width of the protruding block and is smaller than the width of the plugboard;
when the slice handle is matched with the guide opening, two ends of the inserting plate are respectively inserted into the inserting grooves from top to bottom, the protruding blocks are just clamped on the outer side face of the guide opening, and the protruding blocks are flush with the outer side face of the guide opening.
A plurality of flow dividing plates are uniformly distributed around the bottom of the tank body.
The second technical scheme adopted by the invention is that the application method of the experimental treatment device for tissue slices specifically comprises the following steps:
firstly, opening a box cover, inserting a guide column on a handle of a slicing frame into a connecting port of the slicing frame, and placing a tissue slice to be processed on a slice bearing plate of the slicing frame; pinching the two movable columns by hands, putting the slice rack full of tissue slices into the box body, and then taking down the slice rack handles;
when the number of tissue slices to be processed exceeds the bearing capacity of the single-layer slicing frame, a layer of slicing frame can be taken again, the tissue slices are placed above the previous layer of slicing frame through a slicing frame handle, and positioning is carried out between two adjacent layers of slicing frames through the cooperation of positioning grooves and positioning protrusions;
then, dewaxing, cleaning, antigen retrieval, sealing, antibody incubation and staining are sequentially carried out on the tissue slice according to the test requirement;
when tissue slice moving and cleaning operations are performed, the whole slice rack can be taken out through the slice rack handle;
when a certain tissue slice is required to be treated independently, the tissue slice can be taken out of the slice placing groove along with the slice lifting handle;
finally, the processed tissue slice is transferred into a slide box.
The beneficial effects of the invention are as follows:
(1) The device utilizes the principle that the bulges and the grooves are mutually matched to form a stackable slicing frame device, and the slicing frame handle is matched for use, so that the operation of experimenters is simpler and more convenient, and the requirement of batch processing of slices is fully met;
(2) The device ensures that the tissue slice is always in a horizontal state in the experimental process, and the independent operation is not needed in the slice processing process, so that the experimental operation time is saved to the greatest extent, the repeated operation is avoided, and the stability of the slice is ensured;
(3) The device has the advantages that all the slices are in an overall relatively closed environment through the external light-tight closed box body, so that the slices are ensured to be in a relatively stable closed experimental treatment environment, and the light-shielding requirement of partial experimental operation is met;
(4) The device can reduce reagent waste in the tissue slice treatment process, and experimental result errors caused by inconsistent treatment time and intensity during single slice operation, so that the experimental process is more stable.
Drawings
FIG. 1 is a schematic diagram of an experimental treatment apparatus for tissue slices according to the present invention;
FIG. 2 is a schematic view of a section holder in an experimental treatment apparatus for tissue sections according to the present invention;
FIG. 3 is a schematic view showing a structure of a tissue slice experimental treatment apparatus according to the present invention in which a plurality of slice holders are stacked together;
FIG. 4 is a schematic view of the structure of a handle of a section holder in an experimental treatment apparatus for tissue sections according to the present invention;
FIG. 5 is a schematic view showing the internal structure of a handle of a section holder in an experimental treatment apparatus for tissue sections according to the present invention;
FIG. 6 is a schematic view of a slice placement tank in an experimental treatment apparatus for tissue slices according to the present invention;
FIG. 7 is a schematic view showing a state in which a slice handle is engaged with a guide opening in an experimental treatment apparatus for a tissue slice according to the present invention.
In the figure, 1, a box body and 2, a box cover;
3. 3-1 of a slicing frame, namely a frame body;
3-2 parts of slice placing grooves, 3-2-1 parts of groove bodies, 3-2-2 parts of guide openings, 3-2-3 parts of slots, 3-2-4 parts of slice receiving plates, 3-2-5 parts of inserting plates, 3-2-6 parts of protruding blocks and 3-2-7 parts of distributing plates;
3-3 parts of positioning protrusions, 3-4.L parts of cushion blocks, 3-5 parts of connectors and 3-6 parts of guide sleeves;
4. the slicing frame comprises a slicing frame handle, 4-1 parts of a handle frame A,4-2 parts of a handle frame B,4-3 parts of a guiding hole A,4-4 parts of a guiding hole B,4-5 parts of a bracket A,4-6 parts of a bracket B,4-7 parts of a guiding column, 4-8 parts of a movable column and 4-9 parts of a spring;
5. the box body handle, 6, the fixture block, 7, the draw-in groove.
Detailed Description
The invention will be described in detail below with reference to the drawings and the detailed description.
The invention relates to an experimental treatment device for tissue slices, which is shown in fig. 1, and comprises a box body 1, wherein the upper end of the box body 1 is connected with a box cover 2, a plurality of slice frames 3 which can be stacked and placed are arranged in the box body 1, and slice frame handles 4 which can be freely detached can be connected to the slice frames 3. Two ends of the box body 1 are respectively provided with a box body handle 5.
One end of the box body 1 is hinged with one end of the box cover 2, a clamping block 6 is arranged at the center of the other end of the box body 1, a clamping groove 7 is arranged at the center of the other end of the box cover 2, and the clamping block 6 is just clamped at the clamping groove 7, so that the box body 1 and the box cover 2 are closed.
As shown in fig. 2, the slicing frame 3 comprises a frame body 3-1, wherein a plurality of slicing placing grooves 3-2 are regularly divided on the inner side of the frame body 3-1, positioning protrusions 3-3 are respectively arranged at the upper ends of four vertexes of the frame body 3-1, positioning grooves are respectively arranged at the lower ends of four vertexes of the frame body 3-1, and the positioning protrusions 3-1 and the positioning grooves are correspondingly arranged on the frame body 3-1 up and down; the lower ends of the four vertexes of the frame body 3-1 are also respectively provided with an L-shaped cushion block 3-4, and the L-shaped cushion blocks 3-4 are arranged at the periphery of the positioning groove;
a connecting port 3-5 is arranged at the center of one side of the frame body 3-1, and guide sleeves 3-6 are respectively arranged at two opposite sides of the connecting port 3-5.
As shown in fig. 3, when a plurality of slice frames 3 are stacked for use, four positioning projections 3-3 on the slice frame 3 of the upper layer are fitted with four positioning grooves on the slice frame 3 of the adjacent lower layer.
As shown in fig. 4, the slicing frame handle 4 comprises a hollow handle frame A4-1, one end of the handle frame A4-1 is connected with a hollow handle frame B4-2, and the handle frame A4-1 and the handle frame B4-2 are connected to form an L-shaped structure; the opposite sides of the middle part of the handle frame A4-1 are respectively provided with a guiding-out hole A4-3, and the opposite sides of one end of the handle frame B4-2 far away from the handle frame A4-1 are respectively provided with a guiding-out hole B4-4;
as shown in fig. 5, two brackets A4-5 are arranged in parallel in the handle frame A4-1, one ends of the two brackets A4-5 are respectively connected with one ends of the two brackets B4-6, the two brackets B4-6 are arranged in parallel, the brackets B4-6 are positioned in the handle frame B4-2, the other ends of the two brackets B4-6 are respectively connected with one ends of the guide posts 4-7, and the other ends of the two guide posts 4-7 respectively penetrate out of the two guide holes B4-4 and are coaxially connected with the two guide sleeves 3-6 at the connecting port 3-5;
the middle parts of the two brackets A4-5 are respectively provided with a movable column 4-8 in opposite directions, a spring 4-9 is arranged between the two movable columns 4-8, and the two movable columns 4-8 respectively penetrate out of the handle frame A4-1 from the two leading-out holes A4-3.
As shown in FIG. 6, the slice placing groove 3-2 comprises a groove body 3-2-1, one end of the groove body 3-2-1 is provided with a guide opening 3-2-2, two opposite sides of the guide opening 3-2-2 are respectively provided with a slot 3-2-3, and a slice lifting handle is connected in the slot 3-2-3.
The slice handle comprises a slice bearing plate 3-2-4, one end of the slice bearing plate 3-2-4 is horizontally arranged in the groove body 3-2-1, the other end of the slice bearing plate 3-2-4 is connected with a vertically arranged plugboard 3-2-5, and the plugboard 3-2-5 and the slice bearing plate 3-2-4 are connected to form an L-shaped structure;
the center of the outer side of the plugboard 3-2-5 is provided with a protruding block 3-2-6;
the width of the slice bearing plate 3-2-4 is equal to the width of the protruding block 3-2-6 and is smaller than the width of the inserting plate;
when the slice handles are matched with the guide openings 3-2-2, two ends of the inserting plate 3-2-5 are respectively inserted into the inserting grooves 3-2-3 from top to bottom, the protruding blocks 3-2-6 are just clamped on the outer side faces of the guide openings 3-2-2, and the protruding blocks 3-2-6 are flush with the outer side faces of the guide openings 3-2-2, see fig. 7.
A plurality of flow dividing plates 3-2-7 are uniformly distributed around the bottom of the groove body 3-2-1 to prevent the liquid in the tissue slice from accumulating.
The invention also provides a use method of the experimental treatment device for tissue slices, which comprises the following steps:
firstly, the box cover 2 is opened, a guide post 4-7 on a handle 4 of the slicing frame is inserted into a connecting port 3-5 of the slicing frame 3, and a tissue slice to be processed is placed on a slice receiving plate 3-2-4 of the slicing frame 3; the two movable posts 4-8 are pinched by hands, the springs 4-9 are pressed, the slicing frame 3 fully paved with tissue slices is placed in the box body 1, and then the handle 4 of the slicing frame is taken down;
when the number of tissue slices to be processed exceeds the bearing capacity of the single-layer slicing frame 3, a layer of slicing frame 3 can be taken again, the tissue slices are placed above the previous layer of slicing frame 3 through a slicing frame handle 4, and the adjacent two layers of slicing frames 3 are positioned through the cooperation of a positioning groove and a positioning protrusion 3-3;
then, dewaxing, cleaning, antigen retrieval, sealing, antibody incubation and staining are sequentially carried out on the tissue slice according to the test requirement;
when tissue slice moving and cleaning operations are performed, the whole slice rack can be taken out through the slice rack handle;
when a certain tissue slice is required to be treated independently, the tissue slice can be taken out of the slice placing groove along with the slice lifting handle;
finally, the processed tissue slice is transferred into a slide box.
The invention relates to an experimental treatment device for tissue slices, which is characterized by comprising the following steps:
1. the slicing frame adopts a superposition mode. The bottom layer is a cuboid, the size of the bottom layer can accommodate 30 glass slides, four bulges are arranged at the four corners of the bottom layer and can be overlapped with the second layer, the second layer can be overlapped with the third layer again in the same way, and the like, so that the purpose of one-time multiple-volume processing is achieved while the stability of the slices is fully ensured, and the number of layers can be flexibly adjusted according to the number of the processed slices.
2. The slicing handle frame can be connected with each layer of slicing frame, so that slicing layers can be conveniently taken and placed, and the handle can be taken down at any time when slicing operation is not performed, so that the occupied space of the device is reduced;
3. because the slice is always in a horizontal state in the experimental process, the slice is not required to be taken out in sequence in the process of sealing and incubating antibodies in the immunohistochemical experiment, and the slice is directly placed on a single-layer slice frame for operation.
4. If the independent operation of single slice is needed, the slice can be pulled out for processing through the slice handle.
5. The box body provides a closed environment for slicing treatment, and in addition, the box body handle is designed on the outer side of the box body, so that the whole movement is convenient.
6. In the experimental process, some steps need to be operated in a dark place, so the slicing frame is made of opaque ABS plastic material, and the tolerance temperature is-80 ℃.
7. In the operation processes of cleaning, dyeing and the like of the slices, the slices can be simultaneously processed in batches, so that a large amount of operation time is saved, errors of processing time and strength and reagent waste caused by independent operation of the slices are avoided, the slices are cleaned, dyed and the like more uniformly, and the stability of the slices is ensured. The device can be used for experimental operations such as dewaxing, cleaning, sealing, antigen retrieval, antibody incubation, section staining, differentiation and the like of the tissue paraffin sections.
Claims (2)
1. An experimental treatment device for tissue slices, which is characterized in that: the box comprises a box body, wherein the upper end of the box body is connected with a box cover, a plurality of slicing frames capable of being placed in a superposition mode are arranged in the box body, and slicing frames can be connected with slicing frame handles capable of being detached freely;
two ends of the box body are respectively provided with a box body handle;
one end of the box body is hinged with one end of the box cover, a clamping block is arranged at the center of the other end of the box body, a clamping groove is arranged at the center of the other end of the box cover, and the clamping block is just clamped at the clamping groove, so that the box body and the box cover are closed;
the slicing frame comprises a frame body, wherein a plurality of slicing placing grooves are regularly divided on the inner side of the frame body, positioning protrusions are respectively arranged at the upper ends of four vertexes of the frame body, positioning grooves are respectively arranged at the lower ends of the four vertexes of the frame body, and the positioning protrusions and the positioning grooves are correspondingly arranged on the frame body up and down; the lower ends of the four vertexes of the frame body are respectively provided with an L-shaped cushion block which is arranged at the periphery of the positioning groove;
a connecting port is arranged at the center of one side of the frame body, and guide sleeves are respectively arranged at the opposite sides of the connecting port;
when a plurality of slicing frames are overlapped and used, four positioning protrusions on the slicing frame of the upper layer are matched with four positioning grooves on the slicing frame of the adjacent lower layer;
the slicing frame handle comprises a hollow handle frame A, one end of the handle frame A is connected with a hollow handle frame B, and the handle frame A and the handle frame B are connected to form an L-shaped structure; the opposite sides of the middle part of the handle frame A are respectively provided with a guiding-out hole A, and the opposite sides of one end of the handle frame B away from the handle frame A are respectively provided with a guiding-out hole B;
two brackets A are arranged in the handle frame A in parallel, one ends of the two brackets A are respectively connected with one ends of two brackets B, the two brackets B are arranged in parallel, the brackets B are positioned in the handle frame B, the other ends of the two brackets B are respectively connected with one ends of guide posts, and the other ends of the two guide posts respectively penetrate out of the two guide holes B and are coaxially connected with two guide sleeves at the connecting port;
the middle parts of the two brackets A are respectively provided with a movable column in opposite directions, a spring is arranged between the two movable columns, and the two movable columns respectively penetrate out of the handle frame A from the two guide holes A;
the slice placing groove comprises a groove body, one end of the groove body is provided with a guide opening, two opposite sides of the guide opening are respectively provided with a slot, and a slice lifting handle is connected in the slots;
the slice lifting handle comprises a slice bearing plate, one end of the slice bearing plate is horizontally placed in the groove body, the other end of the slice bearing plate is connected with a vertically arranged plugboard, and the plugboard and the slice bearing plate are connected to form an L-shaped structure;
the center of the outer side of the plugboard is provided with a protruding block;
the width of the slice bearing plate is equal to the width of the protruding block and is smaller than the width of the plugboard;
when the slice handles are matched with the guide openings, two ends of the inserting plate are respectively inserted into the slots from top to bottom, the protruding blocks are just clamped on the outer side surfaces of the guide openings, and the protruding blocks are flush with the outer side surfaces of the guide openings;
a plurality of flow dividing plates are uniformly distributed around the bottom of the groove body.
2. The method of claim 1, wherein the step of using the device comprises: the method specifically comprises the following steps:
firstly, opening a box cover, inserting a guide column on a handle of a slicing frame into a connecting port of the slicing frame, and placing a tissue slice to be processed on a slice bearing plate of the slicing frame; pinching the two movable columns by hands, putting the slice rack full of tissue slices into the box body, and then taking down the slice rack handles;
when the number of tissue slices to be processed exceeds the bearing capacity of the single-layer slicing frame, a layer of slicing frame can be taken again, the tissue slices are placed above the previous layer of slicing frame through a slicing frame handle, and positioning is carried out between two adjacent layers of slicing frames through the cooperation of positioning grooves and positioning protrusions;
then, dewaxing, cleaning, antigen retrieval, sealing, antibody incubation and staining are sequentially carried out on the tissue slice according to the test requirement;
when tissue slice moving and cleaning operations are performed, the whole slice rack can be taken out through the slice rack handle;
when a certain tissue slice is required to be treated independently, the tissue slice can be taken out of the slice placing groove along with the slice lifting handle;
finally, the processed tissue slice is transferred into a slide box.
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| CN202010208341.3A CN111389478B (en) | 2020-03-23 | 2020-03-23 | Experimental treatment device for tissue slices and application method thereof |
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| CN107144611A (en) * | 2017-06-30 | 2017-09-08 | 盐城工学院 | A kind of optical electro-chemistry detection method of sulfur dioxide |
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| US5542568A (en) * | 1994-10-26 | 1996-08-06 | Nice-Pak Products, Inc. | Moist tissue package construction and tissue |
| DE10254229B4 (en) * | 2002-11-20 | 2004-10-28 | P.A.L.M. Microlaser Technologies Ag | Positioning device for positioning a collecting device of a laser microdissection system |
| DE10332296B3 (en) * | 2003-07-16 | 2005-04-28 | Fraunhofer Ges Forschung | Substrate stacks, in particular for the cryopreservation of biological samples |
| PL224696B1 (en) * | 2009-03-02 | 2017-01-31 | Imka Spółka Z Ograniczoną Odpowiedzialnością | Method for the handkechief folding |
| GB201613173D0 (en) * | 2016-07-29 | 2016-09-14 | Medical Res Council | Electron microscopy |
| CN207456905U (en) * | 2017-11-17 | 2018-06-05 | 广东医科大学 | It is a kind of can rapid airing pathological section film airing stand |
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| CN107144611A (en) * | 2017-06-30 | 2017-09-08 | 盐城工学院 | A kind of optical electro-chemistry detection method of sulfur dioxide |
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