CN111388609A - Method for extracting functional red yeast rice with high Moraxelin K content - Google Patents
Method for extracting functional red yeast rice with high Moraxelin K content Download PDFInfo
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- CN111388609A CN111388609A CN202010215975.1A CN202010215975A CN111388609A CN 111388609 A CN111388609 A CN 111388609A CN 202010215975 A CN202010215975 A CN 202010215975A CN 111388609 A CN111388609 A CN 111388609A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/062—Ascomycota
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/36—Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/36—Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
- A61K47/40—Cyclodextrins; Derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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Abstract
The invention provides a method for extracting functional red yeast rice with high Moraxelin K content, which comprises the following steps of soaking red yeast rice powder in ethanol, stirring and extracting, carrying out vacuum filtration on an extracting solution, collecting filtrate, soaking residual residues in an ethanol solution, stirring and extracting, carrying out vacuum filtration on a secondary extracting solution, collecting filtrate, combining the filtrates collected twice, extracting an effective component Moraxelin K in the red yeast rice powder, adding an appropriate amount of β cyclodextrin and maltodextrin into the extracted filtrate, carrying out concentration treatment in a rotary evaporator, mixing the concentrated red yeast rice extract with the red yeast rice powder to obtain wet red yeast rice solid, drying and powdering the wet red yeast rice solid, and obtaining the red yeast rice powder with high Moraxelin K content.
Description
[ technical field ] A method for producing a semiconductor device
The invention relates to the technical field of preparation of red yeast rice, in particular to a method for extracting functional red yeast rice with high Moraxelin K content.
[ background of the invention ]
Red yeast rice is also called red yeast rice and red yeast, is a purple red rice yeast which is prepared by fermenting rice serving as a main raw material through monascus, and has medicinal and edible values from ancient times. The compendium of materia medica records that red yeast is mainly used for promoting digestion and activating blood, strengthening spleen and drying stomach, treating red and white dysentery and diarrhea, brewing blood and promoting blood circulation, postpartum lochiorrhea and the like. The red yeast rice can be divided into three types according to the application, the first type is the red yeast rice for coloring, the red yeast rice is divided into specifications by taking different color values as indexes, is mainly used in meat products, is a natural pigment, and is widely used in various countries in the world; the second is red yeast for fermenting and brewing wine; the third is red yeast rice for health care or medicine, also called functional red yeast rice. The functional red yeast rice is a pure natural biological product with the effects of reducing blood cholesterol, reducing blood sugar, reducing blood pressure and preventing cancer, and comprises various bioactive components such as moraxelin K, ergosterol, gamma-aminobutyric acid, natural plant hormones and the like, wherein the content of the moraxelin K determines the quality of the effect of the functional red yeast rice. At present, the content of effective components in the functional red yeast rice prepared by the prior art is low, so that the overall efficacy of the product is influenced, and the extraction method of the functional red yeast rice with high Moraxelin K content is provided.
[ summary of the invention ]
The invention aims to solve the problems in the prior art and provides an extraction method of functional red yeast rice with high Moraxelin K content, which can fully extract the active ingredient Moraxelin K in the functional red yeast rice and improve the drug effect of the product.
In order to realize the purpose, the invention provides a method for extracting functional red yeast rice with high Moraxelin K content, which sequentially comprises the following steps:
step a, adding 500g of red yeast powder into a 5L glass flask, adding 70-80% ethanol solution 4L, soaking for 1.5-3h, stirring and extracting the soaked red yeast powder for 2-3h, performing vacuum filtration on an extracting solution through filter paper, and collecting filtrate;
b, soaking the residue left after the suction filtration in the step a in 3L 70-80% ethanol solution, stirring and extracting for 1-2h, carrying out vacuum filtration on the secondary extracting solution, and collecting filtrate;
step c, combining the two filtrates collected in the step a and the step b;
d, adding β cyclodextrin and maltodextrin which are uniformly mixed with water into a rotary evaporator, transferring the combined collected filtrate obtained in the step c into a vacuum rotary evaporator, and carrying out vacuum concentration in water bath at 60 ℃;
step e, weighing the red yeast rice extract obtained by concentration in the step d, adding red yeast rice powder, and uniformly mixing to obtain red yeast rice solid;
f, putting the mixed red yeast solid into a tray, flattening, putting into an oven, and drying at 60 ℃;
and step g, grinding the dried red yeast solid into fine powder by using a grinding machine, and uniformly mixing to obtain the red yeast powder with high Moraxelin K content.
Preferably, the red rice powder in the step a is soaked in 80% ethanol solution for 2 hours, the temperature of the soaked red rice powder is 50 ℃ when the soaked red rice powder is stirred, and the stirring extraction time is 2 hours.
Preferably, the residue left after suction filtration in the step b is soaked in 80% ethanol solution 3L, and is extracted for 1h at 50 ℃ with stirring.
Preferably, the red rice powder with the content of the moraxelin of 5.0% is selected in the step a, the weight of the β cyclodextrin in the step d is the same as the content of the moraxelin of the red rice powder added in the step a, and the weight of the maltodextrin is 24% of the weight of the red rice powder in the step a.
Preferably, the vacuum concentration process in the step d is divided into 4 times, 0.5L water is added in each time, and the concentrated solution is concentrated until the feed-liquid ratio of the concentrated solution is 1-1.2.
Preferably, the content of the monacolin in the red rice powder added in the step e is 5.0%, and the weight ratio of the red rice extract to the red rice powder is 10: 7.
Preferably, the height of the red yeast solid in the step f after being pressed flat is not more than 0.5 cm.
The method has the beneficial effects that the red yeast powder is soaked in ethanol, the filtrate is collected through secondary filtration, the Moraxelin K serving as an effective component in the red yeast powder can be extracted, a proper amount of β cyclodextrin and maltodextrin are added into the extracted filtrate, concentration treatment is carried out in a rotary evaporator, the concentrated red yeast extract is mixed with the red yeast powder to obtain wet red yeast solid, and the wet red yeast solid is dried and powdered to obtain the red yeast powder with high Moraxelin K content.
The features and advantages of the present invention will be described in detail by way of examples.
[ detailed description ] embodiments
Example one
A method for extracting functional red yeast rice with high Moraxelin K content sequentially comprises the following steps:
step a, taking 500g of monascus powder with the Moraxelin content of 5.0%, adding 80% ethanol solution 4L, soaking for 2h, stirring and extracting for 2h at 50 ℃, carrying out vacuum filtration, and collecting filtrate;
b, extracting the residue after suction filtration with 80% ethanol solution 3L at 50 ℃ for 1h under stirring, carrying out vacuum suction filtration, and collecting the filtrate;
step c, combining the filtrates collected twice in the step a and the step b;
step d, adding 25g of β cyclodextrin and 120g of maltodextrin which are uniformly mixed with water (only a small amount of water is needed to dissolve the dextrin and mix the dextrin into the filtrate) into a rotary evaporator, adding the mixed filtrate obtained in the step c, and carrying out water bath vacuum concentration at 60 ℃;
step e, concentrating for four times, respectively adding 0.5L water, washing off residual solvent, and finally obtaining 136g of red yeast rice extract, wherein the concentration of the red yeast rice extract is 18.2% by fine measurement;
step f, weighing 136g of 5.0% monascus powder, uniformly mixing the monascus powder with the red yeast extract, then putting the mixture into a tray, flattening the mixture until the height is not more than 0.5cm, and drying the mixture at 60 ℃;
and step g, grinding the dried red yeast rice powder into fine powder, and uniformly mixing to obtain 270g of red yeast rice powder, wherein the content of the moraxelin in the red yeast rice powder is 11.5% through detection, and the yield of the red yeast rice is 97%.
Example two
A method for extracting functional red yeast rice with high Moraxelin K content sequentially comprises the following steps:
step a, taking 500g of monascus powder with the Moraxelin content of 5.0%, adding 70% ethanol solution 4L, soaking for 2h, stirring and extracting for 2h at 50 ℃, carrying out vacuum filtration, and collecting filtrate;
b, stirring and extracting the residue after suction filtration for 1h at 50 ℃ by using a 70% ethanol solution 3L, carrying out vacuum suction filtration, and collecting the filtrate;
step c, combining the filtrates collected twice in the step a and the step b;
step d, adding 25g of β cyclodextrin and 120g of maltodextrin which are uniformly mixed with water into a rotary evaporator, adding the collected filtrate combined in the step c, and carrying out vacuum concentration in a water bath at 60 ℃;
step e, concentrating for four times, respectively adding 0.5L water, washing off residual solvent, and finally obtaining 137g of red yeast rice extract, wherein the concentration of the red yeast rice extract is 17.6% by fine measurement;
step f, weighing 137g of 5.0% monascus powder, uniformly mixing the 5.0% monascus powder with the red yeast rice extract, then putting the mixture into a tray, flattening the mixture until the height is not more than 0.5cm, and drying the mixture at 60 ℃;
and step g, grinding the dried red yeast rice powder into fine powder, and uniformly mixing to obtain 273g of red yeast rice powder, wherein the content of the moraxelin in the red yeast rice powder is 11.5% and the yield of the red yeast rice is 97% through detection.
EXAMPLE III
A method for extracting functional red yeast rice with high Moraxelin K content sequentially comprises the following steps:
step a, taking 500g of monascus powder with the Moraxelin content of 5.0%, adding 80% ethanol solution 4L, soaking for 2h, stirring and extracting for 2h at 50 ℃, carrying out vacuum filtration, and collecting filtrate;
b, extracting the residue after suction filtration with 80% ethanol solution 3L at 50 ℃ for 1h under stirring, carrying out vacuum suction filtration, and collecting the filtrate;
step c, combining the filtrates collected twice in the step a and the step b;
d, adding 120g of maltodextrin uniformly mixed with water into a rotary evaporator, adding the collected filtrate combined in the step c, and carrying out vacuum concentration in a water bath at 60 ℃;
step e, concentrating for four times, respectively adding 0.5L water, washing off residual solvent, and finally obtaining 126g of red yeast rice extract, wherein the concentration of the red yeast rice extract is 19.7% by fine measurement;
step f, weighing 126g of 5.0% monascus powder, uniformly mixing the 5.0% monascus powder with the red yeast rice extract, then putting the mixture into a tray, flattening the mixture until the height is not more than 0.5cm, and drying the mixture at 60 ℃;
and step g, grinding the dried red rice powder into fine powder, and uniformly mixing to obtain 249g of red rice powder, wherein detection shows that the content of the moraxelin in the red rice powder is 12.2 percent, and the yield of the red yeast rice is 97 percent.
The samples of example 1 and example 3 were stored in an incubator at 36 ℃ under the same conditions and protected from light, and the contents were measured again on day 14 and day 30, respectively:
after 14 days, the content of moraxelin in the red rice powder of example 1 was 11.1%, the content of moraxelin in the red rice powder of example 3 was 11.5%, and after 30 days, the content of moraxelin in the red rice powder of example 1 was 10.6%, and the content of moraxelin in the red rice powder of example 3 was 10.9%.
The above is only a specific application example of the present invention, and the protection scope of the present invention is not limited in any way. All the technical solutions formed by equivalent transformation or equivalent replacement fall within the protection scope of the present invention.
Claims (7)
1. A method for extracting functional red yeast rice with high Moraxelin K content is characterized by comprising the following steps: the method sequentially comprises the following steps:
step a, adding 500g of red yeast powder into a 5L glass flask, adding 70-80% ethanol solution 4L, soaking for 1.5-3h, stirring and extracting the soaked red yeast powder for 2-3h, performing vacuum filtration on an extracting solution through filter paper, and collecting filtrate;
b, soaking the residue left after the suction filtration in the step a in 3L 70-80% ethanol solution, stirring and extracting for 1-2h, carrying out vacuum filtration on the secondary extracting solution, and collecting filtrate;
step c, combining the two filtrates collected in the step a and the step b;
d, adding β cyclodextrin and maltodextrin which are uniformly mixed with water into a rotary evaporator, transferring the combined collected filtrate obtained in the step c into a vacuum rotary evaporator, and carrying out vacuum concentration in water bath at 60 ℃;
step e, weighing the red yeast rice extract obtained by concentration in the step d, adding red yeast rice powder, and uniformly mixing to obtain red yeast rice solid;
f, putting the mixed red yeast solid into a tray, flattening, putting into an oven, and drying at 60 ℃;
and step g, grinding the dried red yeast solid into fine powder by using a grinding machine, and uniformly mixing to obtain the red yeast powder with high Moraxelin K content.
2. The method for extracting functional red yeast rice with high Moraxelin K content according to claim 1, wherein the method comprises the following steps: and b, soaking the red yeast powder in the 80% ethanol solution for 2 hours in the step a, wherein the temperature of the soaked red yeast powder is 50 ℃ when stirring, and the stirring extraction time is 2 hours.
3. The method for extracting functional red yeast rice with high Moraxelin K content as claimed in claim 1, wherein the residue left after suction filtration in step b is soaked in 80% ethanol solution 3L, and extracted at 50 deg.C under stirring for 1 h.
4. The method for extracting functional red yeast rice with high Moraxelin K content as claimed in claim 1, wherein the red yeast rice powder with Moraxelin content of 5.0% is selected in step a, the weight of the β cyclodextrin in step d is the same as the Moraxelin content of the red yeast rice powder added in step a, and the weight of the maltodextrin is 24% of the weight of the red yeast rice powder in step a.
5. The method for extracting functional red yeast rice with high Moraxelin K content according to claim 1, wherein the vacuum concentration step in step d is divided into 4 times, each time adding 0.5L water, concentrating until the ratio of concentrated solution is 1-1.2.
6. The method for extracting functional red yeast rice with high Moraxelin K content according to claim 1, wherein the method comprises the following steps: the content of the monacolin added in the step e is 5.0 percent, and the weight ratio of the red yeast extract to the red yeast powder is 10: 7.
7. The method for extracting functional red yeast rice with high Moraxelin K content according to claim 1, wherein the method comprises the following steps: and f, the height of the flattened red yeast solid in the step f is not more than 0.5 cm.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114177254A (en) * | 2021-12-10 | 2022-03-15 | 安发（福建）生物科技有限公司 | Method for improving hygroscopicity of red yeast rice extract |
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| CN101407825A (en) * | 2008-10-31 | 2009-04-15 | 江南大学 | Method for producing water-soluble functional red koji powder |
| CN102389535A (en) * | 2011-09-29 | 2012-03-28 | 上海交通大学 | Extraction and separation method and use of active ingredients of red yeast rice |
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- 2020-03-25 CN CN202010215975.1A patent/CN111388609A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101407825A (en) * | 2008-10-31 | 2009-04-15 | 江南大学 | Method for producing water-soluble functional red koji powder |
| CN102389535A (en) * | 2011-09-29 | 2012-03-28 | 上海交通大学 | Extraction and separation method and use of active ingredients of red yeast rice |
Non-Patent Citations (1)
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114177254A (en) * | 2021-12-10 | 2022-03-15 | 安发（福建）生物科技有限公司 | Method for improving hygroscopicity of red yeast rice extract |
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