CN111387221B - 一种天然抗菌抗病毒制剂、制备方法及其用途 - Google Patents
一种天然抗菌抗病毒制剂、制备方法及其用途 Download PDFInfo
- Publication number
- CN111387221B CN111387221B CN202010207341.1A CN202010207341A CN111387221B CN 111387221 B CN111387221 B CN 111387221B CN 202010207341 A CN202010207341 A CN 202010207341A CN 111387221 B CN111387221 B CN 111387221B
- Authority
- CN
- China
- Prior art keywords
- preparation
- mass
- biological enzyme
- accounts
- total mass
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000002360 preparation method Methods 0.000 title claims abstract description 105
- 230000000844 anti-bacterial effect Effects 0.000 title abstract description 20
- 230000000840 anti-viral effect Effects 0.000 title abstract description 12
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims abstract description 57
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 46
- 108090000790 Enzymes Proteins 0.000 claims abstract description 43
- 102000004190 Enzymes Human genes 0.000 claims abstract description 43
- 150000001875 compounds Chemical class 0.000 claims abstract description 32
- 241000894006 Bacteria Species 0.000 claims abstract description 30
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 28
- 241000588694 Erwinia amylovora Species 0.000 claims abstract description 20
- 239000000243 solution Substances 0.000 claims abstract description 20
- 239000001509 sodium citrate Substances 0.000 claims abstract description 19
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims abstract description 19
- 240000000560 Citrus x paradisi Species 0.000 claims abstract description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 10
- 239000000287 crude extract Substances 0.000 claims abstract description 8
- 239000003085 diluting agent Substances 0.000 claims abstract description 8
- 238000001914 filtration Methods 0.000 claims abstract description 7
- 239000011259 mixed solution Substances 0.000 claims abstract description 7
- 239000002245 particle Substances 0.000 claims abstract description 6
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 4
- 239000000706 filtrate Substances 0.000 claims abstract description 4
- 238000012216 screening Methods 0.000 claims abstract description 4
- 238000003756 stirring Methods 0.000 claims abstract description 4
- 238000007865 diluting Methods 0.000 claims abstract description 3
- 238000000034 method Methods 0.000 claims description 17
- 238000006243 chemical reaction Methods 0.000 claims description 14
- 239000007788 liquid Substances 0.000 claims description 14
- 235000014443 Pyrus communis Nutrition 0.000 claims description 12
- 229940107702 grapefruit seed extract Drugs 0.000 claims description 12
- 210000003608 fece Anatomy 0.000 claims description 4
- 230000008014 freezing Effects 0.000 claims description 4
- 238000007710 freezing Methods 0.000 claims description 4
- 239000010871 livestock manure Substances 0.000 claims description 4
- 238000005273 aeration Methods 0.000 claims description 3
- 230000003203 everyday effect Effects 0.000 claims description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 2
- 229920006351 engineering plastic Polymers 0.000 claims description 2
- 239000011152 fibreglass Substances 0.000 claims description 2
- 229910052760 oxygen Inorganic materials 0.000 claims description 2
- 239000001301 oxygen Substances 0.000 claims description 2
- 238000001556 precipitation Methods 0.000 claims description 2
- 239000000469 ethanolic extract Substances 0.000 claims 1
- 239000000284 extract Substances 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 9
- 239000003814 drug Substances 0.000 abstract description 8
- 239000002775 capsule Substances 0.000 abstract description 6
- 229940079593 drug Drugs 0.000 abstract description 4
- 238000004090 dissolution Methods 0.000 abstract description 3
- 229940088598 enzyme Drugs 0.000 description 36
- 235000011187 glycerol Nutrition 0.000 description 14
- 241000220324 Pyrus Species 0.000 description 12
- 239000000203 mixture Substances 0.000 description 12
- 238000009472 formulation Methods 0.000 description 11
- -1 fatty acid flavone Chemical class 0.000 description 10
- 230000005764 inhibitory process Effects 0.000 description 10
- 210000002421 cell wall Anatomy 0.000 description 9
- 239000002131 composite material Substances 0.000 description 9
- 235000014113 dietary fatty acids Nutrition 0.000 description 9
- 229930195729 fatty acid Natural products 0.000 description 9
- 239000000194 fatty acid Substances 0.000 description 9
- 102000016943 Muramidase Human genes 0.000 description 8
- 108010014251 Muramidase Proteins 0.000 description 8
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 8
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 8
- 230000001580 bacterial effect Effects 0.000 description 8
- 229930003944 flavone Natural products 0.000 description 8
- 235000011949 flavones Nutrition 0.000 description 8
- 229960000274 lysozyme Drugs 0.000 description 8
- 239000004325 lysozyme Substances 0.000 description 8
- 235000010335 lysozyme Nutrition 0.000 description 8
- 238000004659 sterilization and disinfection Methods 0.000 description 8
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 8
- 244000052616 bacterial pathogen Species 0.000 description 7
- 238000001514 detection method Methods 0.000 description 7
- 230000002401 inhibitory effect Effects 0.000 description 7
- 230000003385 bacteriostatic effect Effects 0.000 description 6
- 235000013305 food Nutrition 0.000 description 6
- 239000007921 spray Substances 0.000 description 6
- 238000005507 spraying Methods 0.000 description 6
- 241000196324 Embryophyta Species 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- 241000282898 Sus scrofa Species 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 239000011550 stock solution Substances 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 3
- 244000070406 Malus silvestris Species 0.000 description 3
- 240000003768 Solanum lycopersicum Species 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 238000001035 drying Methods 0.000 description 3
- 235000013399 edible fruits Nutrition 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 238000000855 fermentation Methods 0.000 description 3
- 230000004151 fermentation Effects 0.000 description 3
- 230000002265 prevention Effects 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- 206010059866 Drug resistance Diseases 0.000 description 2
- OVRNDRQMDRJTHS-UHFFFAOYSA-N N-acelyl-D-glucosamine Natural products CC(=O)NC1C(O)OC(CO)C(O)C1O OVRNDRQMDRJTHS-UHFFFAOYSA-N 0.000 description 2
- MNLRQHMNZILYPY-MDMHTWEWSA-N N-acetyl-alpha-D-muramic acid Chemical compound OC(=O)[C@@H](C)O[C@H]1[C@H](O)[C@@H](CO)O[C@H](O)[C@@H]1NC(C)=O MNLRQHMNZILYPY-MDMHTWEWSA-N 0.000 description 2
- OVRNDRQMDRJTHS-FMDGEEDCSA-N N-acetyl-beta-D-glucosamine Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O OVRNDRQMDRJTHS-FMDGEEDCSA-N 0.000 description 2
- MBLBDJOUHNCFQT-LXGUWJNJSA-N N-acetylglucosamine Natural products CC(=O)N[C@@H](C=O)[C@@H](O)[C@H](O)[C@H](O)CO MBLBDJOUHNCFQT-LXGUWJNJSA-N 0.000 description 2
- 240000007594 Oryza sativa Species 0.000 description 2
- 235000007164 Oryza sativa Nutrition 0.000 description 2
- 244000000005 bacterial plant pathogen Species 0.000 description 2
- 239000013043 chemical agent Substances 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 229950006780 n-acetylglucosamine Drugs 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 235000009566 rice Nutrition 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 description 1
- 208000035143 Bacterial infection Diseases 0.000 description 1
- 240000007124 Brassica oleracea Species 0.000 description 1
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 description 1
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 description 1
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 description 1
- 241000193417 Brevibacillus laterosporus Species 0.000 description 1
- 244000250392 Cardamine diphylla Species 0.000 description 1
- 235000001250 Cardamine diphylla Nutrition 0.000 description 1
- 244000276331 Citrus maxima Species 0.000 description 1
- 235000001759 Citrus maxima Nutrition 0.000 description 1
- 235000009917 Crataegus X brevipes Nutrition 0.000 description 1
- 235000013204 Crataegus X haemacarpa Nutrition 0.000 description 1
- 235000009685 Crataegus X maligna Nutrition 0.000 description 1
- 235000009444 Crataegus X rubrocarnea Nutrition 0.000 description 1
- 235000009486 Crataegus bullatus Nutrition 0.000 description 1
- 235000017181 Crataegus chrysocarpa Nutrition 0.000 description 1
- 235000009682 Crataegus limnophila Nutrition 0.000 description 1
- 235000004423 Crataegus monogyna Nutrition 0.000 description 1
- 240000000171 Crataegus monogyna Species 0.000 description 1
- 235000002313 Crataegus paludosa Nutrition 0.000 description 1
- 235000007106 Crataegus suborbiculata Nutrition 0.000 description 1
- 241000073432 Crataegus suborbiculata Species 0.000 description 1
- 235000009840 Crataegus x incaedua Nutrition 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 241000223218 Fusarium Species 0.000 description 1
- IAJILQKETJEXLJ-UHFFFAOYSA-N Galacturonsaeure Natural products O=CC(O)C(O)C(O)C(O)C(O)=O IAJILQKETJEXLJ-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 241000282414 Homo sapiens Species 0.000 description 1
- 235000005087 Malus prunifolia Nutrition 0.000 description 1
- 235000011430 Malus pumila Nutrition 0.000 description 1
- 235000015103 Malus silvestris Nutrition 0.000 description 1
- 206010027146 Melanoderma Diseases 0.000 description 1
- 244000179970 Monarda didyma Species 0.000 description 1
- 235000010672 Monarda didyma Nutrition 0.000 description 1
- 241000232299 Ralstonia Species 0.000 description 1
- 241000813090 Rhizoctonia solani Species 0.000 description 1
- 241000607128 Salmonella enterica subsp. enterica serovar Infantis Species 0.000 description 1
- 241000218483 Streptomyces lydicus Species 0.000 description 1
- 208000025865 Ulcer Diseases 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 235000013202 a hawthorn Nutrition 0.000 description 1
- IAJILQKETJEXLJ-QTBDOELSSA-N aldehydo-D-glucuronic acid Chemical compound O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)C(O)=O IAJILQKETJEXLJ-QTBDOELSSA-N 0.000 description 1
- 230000003042 antagnostic effect Effects 0.000 description 1
- 239000003443 antiviral agent Substances 0.000 description 1
- 235000021016 apples Nutrition 0.000 description 1
- 239000003899 bactericide agent Substances 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 239000000490 cosmetic additive Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000001066 destructive effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 230000000857 drug effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 239000002778 food additive Substances 0.000 description 1
- 244000000004 fungal plant pathogen Species 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 229940097043 glucuronic acid Drugs 0.000 description 1
- 230000009036 growth inhibition Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000000415 inactivating effect Effects 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 210000003097 mucus Anatomy 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000007918 pathogenicity Effects 0.000 description 1
- 235000021017 pears Nutrition 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 231100000397 ulcer Toxicity 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/36—Rutaceae [Rue family], e.g. lime, orange, lemon, corktree or pricklyash
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/3463—Organic compounds; Microorganisms; Enzymes
- A23L3/3472—Compounds of undetermined constitution obtained from animals or plants
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/3463—Organic compounds; Microorganisms; Enzymes
- A23L3/3481—Organic compounds containing oxygen
- A23L3/349—Organic compounds containing oxygen with singly-bound oxygen
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/3463—Organic compounds; Microorganisms; Enzymes
- A23L3/3481—Organic compounds containing oxygen
- A23L3/3499—Organic compounds containing oxygen with doubly-bound oxygen
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/3463—Organic compounds; Microorganisms; Enzymes
- A23L3/3571—Microorganisms; Enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2462—Lysozyme (3.2.1.17)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01017—Lysozyme (3.2.1.17)
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Environmental Sciences (AREA)
- Polymers & Plastics (AREA)
- Agronomy & Crop Science (AREA)
- Food Science & Technology (AREA)
- Nutrition Science (AREA)
- General Chemical & Material Sciences (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Mycology (AREA)
- Botany (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Pest Control & Pesticides (AREA)
- Virology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
本发明属于医药技术领域，公开了一种天然抗菌抗病毒制剂、制备方法及其用途，所示制剂的制备方法包括以下步骤：1)筛选柚子类种籽，粉碎至一定粒度、采用乙醇为提取剂通过搅拌的方式提取，得到粗提液，将所述粗提液过滤得到滤液；再通过回收乙醇，得到提取液；2)向步骤1)中的提取液中加入甘油，再添加水进行稀释一定倍数，得到稀释液，向所述稀释液中加入柠檬酸、柠檬酸钠和复合生物酶，得到混合溶液。本发明的制剂利用各组分协同，首先快速溶解其荚膜，再进一步促进细胞溶解，有效抑制梨火疫病病菌，同时能够快速的针对普通细菌起效，具有广谱性的抗菌效果。
Description
技术领域
本发明属于医药技术领域，具体涉及一种天然抗菌抗病毒制剂、制备方法及其用途。
背景技术
梨火疫病病菌是一种具有毁灭性的植物病原细菌，寄主范围很广，能危害梨、苹果、山楂、木旬子、李等40多个属220多种植物，造成严重的经济损失。感染后的主要症状为：花器呈萎蔫状，深褐色，并向下蔓延至花柄，使花柄呈水渍状。叶片发病，先从叶缘开始变黑色，然后沿叶脉发展，最终全叶变黑、凋萎。病果初生水渍状斑，后变暗褐色，并有黄色粘液溢出，最后病果变黑而干缩。枝干被害，初呈水渍状，有明显边缘，后病部凹陷出现溃疡状呈褐色至黑色。梨火疫病病菌被视为最难控制的细菌性病害之一，严重感染可能会杀死一个季节内的全部树木。
目前，梨火疫病的防治主要是采用化学药剂链霉素，化学防治尽管能快速消除病原菌，但易造成植株耐药性、污染环境、破坏生态平衡和威胁人畜的健康和安全。因此，寻求绿色、环保的火疫病防治方法是现代农业发展中的一项重要任务。生物防治作为一种绿色安全的防治方法，受到越来越多的关注。
经检索，现有技术已有相关的申请案公开，如中国专利申请号为2019102668000，公开日期为2019年6月4日的申请案公开了一种利迪链霉菌在防治植物火疫病中的应用，该菌株对火疫病病原菌解淀粉欧文氏菌具有显著的拮抗效果，对蔷薇科植物尤其是梨、苹果和海棠等经济作物火疫病具有良好的防治效果，该申请案以生物菌作为药剂，较化学农药对植物病害的防治相比，效果显著，对环境无污染，有利于绿色、无公害产品的生产，可用于制备防治火疫病的功能性生防菌剂。
再如中国专利申请号为2014107962089，公开日期为2015年4月1日的申请案公开了一株侧孢短芽孢杆菌及其应用，并公开了该菌株具有如下特征：对多种植物病原真菌(番茄枯萎病菌、辣椒根腐茄柄镰刀菌、番茄灰霉病菌、水稻纹枯病菌、甘蓝黑斑病菌、梨炭疽病菌和梨轮纹病菌)及植物病原细菌(番茄青枯病菌、梨火疫病菌和水稻细条斑病菌)均具有较强的室内平板抑制作用；是微生物农药研发的有用资源，具有潜在的应用前景。
上述申请案的方法采用生物菌作为防治药物，相比化学类药物虽然能够避免环境污染，然而其需要的投入量一般较大，而且由于大量菌种的投加同样会有引发菌体泛滥的情况，具有破坏生态平衡的风险。
基于现有技术的缺陷，亟需发明一种既是绿色、无公害的，又可以避免菌体泛滥的防治火疫病的制剂。
发明内容
1.要解决的问题
针对现有技术中防治梨火疫病的方法通常采用化学药剂，易造成植株耐药性、污染环境、破坏生态平衡的缺陷，采用生物菌防治方法容易导致菌体泛滥，同样具有破坏生态平衡的风险，本发明提供了含有柚子类种籽提取液、柠檬酸、柠檬酸钠、甘油和复合生物酶的制剂，利用各组分协同，首先快速溶解其荚膜，再进一步促进细胞溶解，有效抑制梨火疫病病菌，对环境无污染，有利于绿色、无公害产品的生产。
进一步的，本发明的发明目的在于提供一种广谱性的抗菌制剂，不仅能够快速针对特殊细菌的防治，同时对于普通细菌抑制也更加快速有效。
2.技术方案
为了解决上述问题，本发明所采用的技术方案如下：
本发明提供了一种天然抗菌抗病毒制剂，所述制剂包括柚子类种籽提取液、柠檬酸、柠檬酸钠、甘油和复合生物酶。
作为本发明更进一步的改进，所述柠檬酸的质量占制剂总质量的0.1％～5％，柠檬酸钠的质量占制剂总质量的0.05％～1％，所述复合生物酶的质量占制剂总质量的1～30％，所述柚子类种籽提取液的质量占制剂总质量的0.1％～1％，所述甘油的质量占制剂总质量的0.2％～2％。
作为本发明更进一步的改进，所述的天然抗菌抗病毒制剂的制备方法，所述方法包括以下步骤：
1)筛选柚子类种籽，粉碎至一定粒度、采用乙醇为提取剂通过搅拌的方式提取，得到粗提液，将所述粗提液过滤得到滤液；再通过回收乙醇，得到提取液；
2)向步骤1)中的提取液中加入甘油，再添加水稀释一定倍数，得到稀释液，向所述稀释液中加入柠檬酸、柠檬酸钠和复合生物酶，得到混合溶液。
优选的，如果步骤2)中得到的混合溶液pH值较高，可以使用碱性水调节混合溶液pH到5.5～8的范围内。
上述混合溶液经过高温灭菌处理，制备成为抗菌产品。可直接以液体形式使用，可通过干燥处理制备成干燥制品，使用时进行加水稀释。
作为本发明更进一步的改进，所述复合生物酶是将猪粪尿原液进行发酵得到发酵液，再将发酵液过滤得到，所述的复合生物酶中含有溶菌酶。
作为本发明更进一步的改进，所述复合生物酶提取方法包括以下步骤：
步骤a)将猪粪尿原液投入多个反应器中，依次按照好氧-好氧-好氧-兼氧-厌氧-好氧-兼氧-厌氧-好氧-兼氧-厌氧-好氧工艺进行处理，经复合发酵后得到其代谢产物的液体制剂；前三个反应器内分别设有微纳米曝气装置；
步骤b)将得到的液体制剂进行过滤，取上清液作为复合生物酶使用，所述复合生物酶中微生物含量低于1％。
作为本发明更进一步的改进，所述种籽在粉碎前需将其在-25℃～-80℃下进行快速冷冻处理5小时以上。
作为本发明更进一步的改进，所述步骤1)中以乙醇为提取剂时保持料液比为1：(10～60)。
作为本发明更进一步的改进，所述步骤1)中的柚子类种籽含有葡萄柚种籽和/或青柚种籽，和/或所述步骤1)中的粉碎粒度小于5mm。
作为本发明更进一步的改进，所述制剂用于灭活病毒和细菌。
作为本发明更进一步的改进，所述细菌包括梨火疫病病菌、婴儿沙门氏菌和大肠杆菌。优选为梨火疫病病菌。
本发明制剂具有广谱性的抗菌抗病毒作用，且成分均为纯天然成分，非常安全，可以制备成为抗菌抗病毒药物、食品和化妆品添加剂、医用冲洗液或医用喷雾中的任意一种。
制备成为喷雾的推荐使用方法为：使用超细喷雾装置(喷雾颗粒小于10微米)喷出干雾，这种方法可使喷雾与空气接触的比表面积大，作用效率高，运行成本低；不仅用于食品工厂的卫生管理，超市及各类蔬菜水果卖场，各种食品的灭菌消毒，还可用于各种人类的起居空间的灭菌消毒(如家，办公室，学校，会议场所，飞机，火车，高铁，汽车，轮船，餐厅，宾馆)。
3.有益效果
相比于现有技术，本发明的有益效果为：
(1)本发明的天然抗菌抗病毒制剂，制备过程中，首先从柚子类种籽中提取得到主要成分为脂肪酸类黄酮的提取液，然后在提取液中加入以利于主成分(脂肪酸类黄酮)稳定的甘油，最后加入柠檬酸、柠檬酸钠和复合生物酶(含有溶菌酶)进行复配制备而成，本发明制剂中各成分进行组合，能够协同作用于梨火疫病致病菌的外层荚膜，首先快速溶解致病菌的荚膜，再进一步促进细胞溶解，有效抑制梨火疫病病菌。本发明的制剂对环境无污染，有利于绿色、无公害产品的生产，可用于制备防治火疫病的功能性制剂。
(2)本发明的天然抗菌抗病毒制剂，具有广谱性的抗菌功能，其主要作用原理如下：1)该制剂含有能够抑制一般细菌的复合生物酶(其含有溶菌酶和多种复合酶)，通过溶菌酶可以破坏一般细菌的细胞壁中的N-乙酰胞壁酸和N-乙酰氨基葡萄糖之间的β-1,4糖苷键，使细胞壁破裂、细菌溶解，因此复合生物酶针对普通细菌本身即可起到有效抑制作用，而在脂肪酸类黄酮与柠檬酸、柠檬酸钠的协同作用下，能够加速细菌细胞壁的溶解，使其快速被分解，缩短作用起效时间；2)对于自然界还存在的特殊细菌(如梨火疫病致病菌)，其细胞壁被特殊结构-荚膜保护，而荚膜成分因不同菌种而异(主要是由葡萄糖与葡萄糖醛酸组成的聚合物，也有含多肽与脂质)。这成为该类病原菌难以通过溶解细胞壁来抑制的原因之一，本发明的制剂通过从柚子类种籽中提取得到的脂肪酸类黄酮与柠檬酸、柠檬酸钠组合，促进该类病原菌的荚膜被快速破坏，再通过脂肪酸类黄酮进一步灭活外层荚膜被去掉的病原菌，同时制剂中含有的复合生物酶也在该阶段发挥了强化杀菌的功能。因此，本发明的制剂不仅是针对普通的细菌还是外层具有荚膜的特殊细菌均可以发挥有效的杀菌作用，发挥作用快速有效。
(3)本发明的天然抗菌抗病毒制剂，在提取液中加入甘油成分以保证提取得到的主成分脂肪酸类黄酮能够稳定存在于该提取液体系，且使提取液不易挥发，以在最终的提取液中获得更多的有效成分，持续生效时间更长，更有利于杀菌杀毒作用的发挥。另外，本发明的方法在种籽粉碎前需要进行将其在-25℃～-80℃下进行冷冻处理，可以使大量的脂肪酸类黄酮物质聚集于种籽的核心部分，并且在冷冻状态下进行粉碎，易于更多的脂肪酸类黄酮物质乙醇方式提取，保留更多的有效成分。
附图说明
图1为实施例1中各制剂样品对梨火疫病病菌生长的抑制效果对比图；
图2为复合生物酶对梨火疫病病菌生长的抑制效果图；
图中，a代表实施例1中的制剂样品1的抑菌结果；b代表实施例1中的制剂样品2的抑菌结果；c代表实施例1中的制剂样品3的抑菌结果；d代表实施例1中的制剂样品4的抑菌结果。
具体实施方式
下面结合具体实施例对本发明进一步进行描述。
实施例1
本发明的天然抗菌抗病毒制剂，制备方法包括如下步骤：
1)筛选葡萄柚种籽、清洗，晒干后先在-25℃～-80℃下对种籽进行冷冻5小时，再进行粉碎，粉碎粒度小于5mm，将粉碎好的种籽装入提取罐中，加入70％乙醇作为提取剂，25℃搅拌条件下提取，保持料液比在1：(10～60)之间，提取120分钟后得到粗提液，将所述粗提液过滤得到滤液；再通过回收乙醇，得到提取液；
2)向所述提取液中加入甘油，再添加水进行稀释，得到稀释液，在所述稀释液中加入柠檬酸、柠檬酸钠和复合生物酶，得到混合溶液。
所述复合生物酶为酶液形式，具体制备过程如下：采用多个工程塑料或玻璃钢制的大反应桶，多个反应桶依次按照好氧-好氧-好氧-兼氧-厌氧-好氧-兼氧-厌氧-好氧-兼氧-厌氧-好氧(O/O/O-A/A/O-A/A/O-A/A/O)间歇设置并连接，前三个反应桶中均设有微纳米曝气装置，控制反应桶中溶解氧在2mg/L以上；每天从第一个反应桶中导入猪粪尿原液，以自然溢流方式依次进入下一反应桶中，三个月后从最后的反应桶中最终溢流出生物酶液，再经沉淀过滤桶过滤纯化后制得复合生物酶，经检验，上述制备的复合生物酶中含有溶菌酶和多种复合酶，微生物含量低于1％。
依据提取液含量不同，以制备不同浓度的制剂样品进行抑菌实验，本实施例中分别记为制剂样品1～4，所述制剂样品1中，柚子类种籽提取液的质量占制剂总质量的0.8～1％，所述甘油的质量占制剂总质量的1.6％～2％。所述柠檬酸的质量占制剂总质量的0.1％～5％，柠檬酸钠的质量占制剂总质量的0.05％～1％，所述复合生物酶的质量占制剂总质量的1～30％。
所述制剂样品2中，柚子类种籽提取液的质量占制剂总质量的0.5～0.8％，所述甘油的质量占制剂总质量的1％～1.6％，所述柠檬酸的质量占制剂总质量的0.1％～5％，柠檬酸钠的质量占制剂总质量的0.05％～1％，所述复合生物酶的质量占制剂总质量的1～30％。
所述制剂样品3中，柚子类种籽提取液的质量占制剂总质量的0.1～0.5％，所述甘油的质量占制剂总质量的0.2％～1％，所述柠檬酸的质量占制剂总质量的0.1％～5％，柠檬酸钠的质量占制剂总质量的0.05％～1％，所述复合生物酶的质量占制剂总质量的1～30％。
所述制剂样品4中，柚子类种籽提取液的质量占制剂总质量的0.02～0.05％，所述甘油的质量占制剂总质量的0.04％～0.1％，所述柠檬酸的质量占制剂总质量的0.1％～5％，柠檬酸钠的质量占制剂总质量的0.05％～1％，所述复合生物酶的质量占制剂总质量的1～30％。
抑菌实验
通过“滤纸片法”测定复合生物酶以及不同浓度的制剂样品对梨火疫病病菌的生长抑制。采用复合生物酶作为对比实验，目的在于：所述的复合生物酶中含有溶菌酶，可通过溶解大部分的细菌细胞壁使细菌被破坏，针对不同种类的细菌，通常具有抑菌效果。
供试菌株
梨火疫病病菌Erwiniaamylovora，从新疆库尔勒市的香梨产区正在受梨火疫病病菌侵害的山楂样本上分离得到菌株XJSZ0102，经准确鉴定和致病力测定后超低温保存，将其作为实验对象。
供试药剂：制剂样品1，制剂样品2，制剂样品3、制剂样品4，复合生物酶。
试验方法
分别取本发明的制剂1～4与复合生物酶制剂进行抑菌试验。吸取1ml(108CFU/ml)菌悬液在直径为90mm的NA平板培养基上均匀涂开，吸出多余菌液。晾干后，将直径为6mm的灭菌滤纸片分别充分浸泡上述药液，滴落多余药液后置于上述涂菌平板培养基上，每皿均匀放置3片，重复3次，以无菌水作为对照。置于28℃生化培养箱中培养24h后，采用十字交叉法测量抑菌圈直径。
结果与分析
试验结果表明，复合生物酶对梨火疫病病菌的药效与阴性对照无差异，对病原菌的生长无抑制作用。虽然复合生物酶中含有的溶菌酶可以通过破坏一般细菌的细胞壁中的N-乙酰胞壁酸和N-乙酰氨基葡萄糖之间的β-1,4糖苷键，使细胞壁破裂、细菌溶解，针对普通细菌可起到有效抑制作用，然而针对含有荚膜的特殊细菌(梨火疫病病菌)则难以发挥作用，具体见图2。本发明的制剂1～3均对梨火疫病病菌的生长有较好的抑制作用，具体见图1。
表1为不同制剂样品对梨火疫病病菌的抑菌圈直径统计，根据表1可知，制剂样品1的平均抑菌圈直径约为17mm，制剂样品2的平均抑菌圈直径约为9.2mm，制剂样品3的平均抑菌圈直径约为7.8mm。制剂样品4未体现出抑菌作用。
由上述结果可知，柚子类种籽提取液对梨火疫病病菌的荚膜破坏起到重要作用，其浓度越高，抑菌效果越好，加入含量至0.05％以下时无法发挥抑菌作用，本发明通过从柚子类种籽中提取得到的脂肪酸类黄酮与柠檬酸、柠檬酸钠组合，促进该类病原菌的荚膜被快速破坏，再通过脂肪酸类黄酮进一步灭活外层荚膜被去掉的病原菌，同时制剂中含有的复合生物酶也在该阶段发挥了强化杀菌的功能。本发明的制剂不仅是针对普通的细菌还是外层具有荚膜的特殊细菌均可以发挥有效的杀菌作用，发挥作用快速有效。
表1不同制剂样品对梨火疫病病菌抑菌圈直径(含滤纸片直径6mm)
注：平均抑菌圈数值为平均值±标准误，同列数据后不同字母表示经Duncan新复极差法检验在P＜0.05水平差异显著。
实施例2
按照实施例1的制备方法制备本发明的制剂，不同之处在于：所述柚子类种籽提取液的质量占制剂总质量的0.2％，所述甘油的质量占制剂总质量的0.4％。所述柠檬酸的质量占制剂总质量的0.5％，柠檬酸钠的质量占制剂总质量的0.1％，所述复合生物酶的质量占制剂总质量的5％。针对普通的细菌，复合生物酶在脂肪酸类黄酮与柠檬酸、柠檬酸钠的配合下，更能够加速细菌细胞壁的溶解，使其快速被分解。
将本实施例的制剂制备成喷雾，使用普通喷雾器对餐厅厨房(面积约50平方米)进行灭菌处理：喷雾数量每平方米30mL，总用量为1500mL。
检测部位为：食材储藏间、砧板周边区域、菜品制作台；测量仪器：LumitesterPD30；开始时检测值6350RLU，喷雾60秒停止5分钟后降到865RLU，停止10分钟后降至230RLU(1000RLU以下符合日本食品加工及餐饮业的卫生标准中一般杂菌标准)。之后持续观测一周，均可保持在500RLU以下。经过三个月以后，每天打烊前喷雾每平米15mL，持续监测三个月，一般杂菌值一直保持在300RLU以下。
实施例3
本实施例为采用本发明的产品进行家居室内消毒的实施例：
按照实施例1的制备方法制备本发明的制剂，不同之处在于：所述柚子类种籽提取液的质量占制剂总质量的0.1％，所述甘油的质量占制剂总质量的0.2％，所述甘油的质量占制剂总质量的0.4％。所述柠檬酸的质量占制剂总质量的0.5％，柠檬酸钠的质量占制剂总质量的0.1％，所述复合生物酶的质量占制剂总质量的5％。
制备成为普通喷雾，用于家居室内消毒：使用普通喷雾器对家居室内(面积约120平方米)进行灭菌处理：喷雾数量每平方米10mL升，总用量为1200mL。检测部位为：厨房，卫生间，起居室，卧室，储藏间等；测量仪器：Lumitester PD30；检测值开始时检测值最高3560RLU，喷雾60秒停止5分钟后降到751RLU，停止10分钟后降至246RLU(1000RLU以下符合日本食品加工及餐饮业的卫生标准)。之后持续观测一周，均可保持在300RLU以下。三个月之后，每3天喷雾一次每平米10毫升，持续监测一个月，一般杂菌值一直保持在210RLU以下。
Claims (6)
1.一种天然抗梨火疫病病菌制剂，其特征在于：所述制剂包括葡萄柚种籽乙醇提取液、柠檬酸、柠檬酸钠、甘油和复合生物酶，所述柠檬酸的质量占制剂总质量的0.1%~5%，柠檬酸钠的质量占制剂总质量的0.05%~1%，所述复合生物酶的质量占制剂总质量的1~30%，所述葡萄柚类种籽提取液的质量占制剂总质量的0.1%~1%，所述甘油的质量占制剂总质量的0.2%~2%，复合生物酶具体制备过程如下：采用多个工程塑料或玻璃钢制的大反应桶，多个反应桶依次按照好氧-好氧-好氧-兼氧-厌氧-好氧-兼氧-厌氧-好氧-兼氧-厌氧-好氧间歇设置并连接，前三个反应桶中均设有微纳米曝气装置，控制反应桶中溶解氧在2mg/L以上，每天从第一个反应桶中导入猪粪尿原液，以自然溢流方式依次进入下一反应桶中，三个月后从最后的反应桶中最终溢流出生物酶液，再经沉淀过滤桶过滤纯化后制得复合生物酶。
2.权利要求1所述的天然抗梨火疫病病菌制剂的制备方法，其特征在于：所述方法包括以下步骤：
1）筛选葡萄柚类种籽，粉碎至一定粒度、采用乙醇为提取剂通过搅拌的方式提取，得到粗提液，将所述粗提液过滤得到滤液，再通过回收乙醇，得到葡萄柚类种籽的提取液；
2）向所述提取液中加入甘油，再添加水稀释一定倍数，得到稀释液，向所述稀释液中加入柠檬酸、柠檬酸钠和复合生物酶，得到混合溶液。
3.根据权利要求2所述的天然抗梨火疫病病菌制剂的制备方法，其特征在于：所述种籽在粉碎前需将其在-25℃~ -80℃下快速冷冻处理5小时以上。
4.根据权利要求2所述的天然抗梨火疫病病菌制剂的制备方法，其特征在于：所述步骤1）中以乙醇为提取剂时保持料液比为1：（10~60）。
5.根据权利要求2所述的天然抗梨火疫病病菌制剂的制备方法，其特征在于：所述步骤1）中的粉碎粒度小于5mm。
6.权利要求1所述的天然抗梨火疫病病菌制剂在抗梨火疫病病菌中的用途。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010207341.1A CN111387221B (zh) | 2020-03-23 | 2020-03-23 | 一种天然抗菌抗病毒制剂、制备方法及其用途 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010207341.1A CN111387221B (zh) | 2020-03-23 | 2020-03-23 | 一种天然抗菌抗病毒制剂、制备方法及其用途 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN111387221A CN111387221A (zh) | 2020-07-10 |
| CN111387221B true CN111387221B (zh) | 2021-06-15 |
Family
ID=71412199
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010207341.1A Active CN111387221B (zh) | 2020-03-23 | 2020-03-23 | 一种天然抗菌抗病毒制剂、制备方法及其用途 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111387221B (zh) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MX2020014292A (es) * | 2020-12-18 | 2022-06-20 | Jose Luis Escobedo Embriz | Mezcla y pesticida organicos, y metodo para el control de fitopatogenos. |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR910019542A (ko) * | 1990-05-25 | 1991-12-19 | 히로에 오가와 | 감귤 종자 추출물을 함유하는 저온 저장 가공 식품, 감귤 종자 추출물을 함유하는 조성물 및 그의 용도 |
| KR20030070487A (ko) * | 2002-02-25 | 2003-08-30 | 김대승 | 세정항균탈취액의 제조방법 |
| US20120263699A1 (en) * | 2011-04-13 | 2012-10-18 | Nzymsys, Inc. | Compositions and Methods for Preservation of Fresh Produce |
| WO2012139146A1 (de) * | 2011-04-13 | 2012-10-18 | Technische Universität Wien | Verfahren zur bekämpfung von bakteriellen infektionen bei einer pflanze unter verwendung von flavonoiden |
| WO2013050662A1 (fr) * | 2011-10-04 | 2013-04-11 | Xeda International | Procède de traitement fongicide et/ou bactéricide de souches résistantes au moyen d'huile(s) essentielle(s) |
| CN108358692A (zh) * | 2018-02-12 | 2018-08-03 | 浙江亲水园生物科技有限公司 | 一种利用畜禽粪污的液肥及其制备方法及其应用方法 |
| CN108840535A (zh) * | 2018-06-30 | 2018-11-20 | 贵州织金丰金农业科技发展有限公司 | 一种畜禽粪便除臭的生物菌剂 |
| US10285922B1 (en) * | 2019-01-24 | 2019-05-14 | Bruce Wayne Miller | Topical exfoliating formulation |
-
2020
- 2020-03-23 CN CN202010207341.1A patent/CN111387221B/zh active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN111387221A (zh) | 2020-07-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Kilani | Antibacterial assessment of whole stem bark of Vitex doniana against some enterobactriaceae | |
| Abdulkadir et al. | Phytochemical screening and antimicrobial activities of ethanolic extracts of Moringa oleifera Lam on isolates of some pathogens | |
| Henderson et al. | Antimicrobial activity of lemon (Citrus limon) peel extract against Escherichia coli | |
| Li et al. | Antibacterial activity and main action pathway of benzyl isothiocyanate extracted from papaya seeds | |
| Khan et al. | Phytochemical screening, antimicrobial and antioxidant activity determination of citrus maxima peel | |
| Gómez‐Aldapa et al. | Behavior of thirteen foodborne bacteria on whole Hass avocado and potential of roselle calyx extracts as alternative disinfectant agents of avocado | |
| CN111387221B (zh) | 一种天然抗菌抗病毒制剂、制备方法及其用途 | |
| Russell | Microbiological control of raw materials | |
| CN101744042A (zh) | 一种用于水果的天然食品保鲜剂及其制备方法 | |
| Mitsagga et al. | Antimicrobial properties of lyophilized extracts of olive fruit, pomegranate and orange peel extracts against foodborne pathogenic and spoilage bacteria and fungi in vitro and in food matrices | |
| Budaraga et al. | Liquid Smoke Antimicrobial Test of Cocoa Fruit Peel Against Eschericia Coli and Staphylococcus Aureus Bacteria | |
| Yap et al. | Antibacterial activity of polyphenol-rich extract of selected wild honey collected in Sabah, Malaysia | |
| KR20030037094A (ko) | 천연 항균 조성물 | |
| Zaini | Antibacterial effectiveness of Morinda citrifolia L. extract on Salmonella typhi bacteria using serial dilution method with 15-60 minutes contact time | |
| Sheh-Hong et al. | Assessment of anticandidal activity and cytotoxicity of root extract from Curculigo latifolia on pathogenic | |
| KR101340597B1 (ko) | 천연 휴믹 물질로부터 다기능성 추출물 제조방법 | |
| Seghosime et al. | Potential use of Mangifera indica seed kernel and Citrus aurantiifolia seed in water disinfection | |
| Foronda et al. | Anti-Bacterial, Cytotoxicity, and Antioxidant Properties of the Isolated Flavonoids Extract from White Dragon Fruit (Hylocereus undatus) Peels and Flesh | |
| Yu et al. | Study on antibacterial and antioxidant activities of Salicornia herbacea extracts | |
| Kirui et al. | Chemical assessment and antimicrobial activity of solvent extracts from Kalanchoe densiflora | |
| Restuati et al. | Effect of leaf extract buasbuas (Premna pubescens Blume) for against of bacteria growth Staphylococcus aureus and Salmonella sp in vitro | |
| Hasan et al. | The potential of isolates endophytic fungi from the leaves of henna plant (Lawsonia inermis) toward the growth of Candida albicans and Staphylococcus aureus | |
| Nguyen et al. | Comparative study of the antibacterial and anti-inflammatory activities of the seed coat vs. seed kernel extracts from the plant Mangifera indica L. in inflammatory acne treatment | |
| Zeleke et al. | The In Vitro Antibacterial Sensitivity Test of Selected, Medicinal Plants | |
| Frial-McBride | Extraction of resins from Capsicum annuum var. longum (Siling haba) for the study of their potential anti-microbial activities |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |