CN111358706A - Preparation method of alcohol-fibroin gel washing-free disinfectant - Google Patents
Preparation method of alcohol-fibroin gel washing-free disinfectant Download PDFInfo
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- CN111358706A CN111358706A CN202010287004.8A CN202010287004A CN111358706A CN 111358706 A CN111358706 A CN 111358706A CN 202010287004 A CN202010287004 A CN 202010287004A CN 111358706 A CN111358706 A CN 111358706A
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- 239000000645 desinfectant Substances 0.000 title claims abstract description 62
- 238000002360 preparation method Methods 0.000 title claims abstract description 27
- 108010022355 Fibroins Proteins 0.000 claims abstract description 95
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 65
- 108010013296 Sericins Proteins 0.000 claims abstract description 32
- 238000003756 stirring Methods 0.000 claims abstract description 23
- 238000002156 mixing Methods 0.000 claims abstract description 20
- 239000000243 solution Substances 0.000 claims description 122
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 35
- 229910001868 water Inorganic materials 0.000 claims description 35
- 239000000835 fiber Substances 0.000 claims description 19
- 238000000034 method Methods 0.000 claims description 11
- 238000005406 washing Methods 0.000 claims description 10
- 238000000502 dialysis Methods 0.000 claims description 9
- 238000004090 dissolution Methods 0.000 claims description 9
- 238000001035 drying Methods 0.000 claims description 9
- 238000011049 filling Methods 0.000 claims description 9
- 150000003384 small molecules Chemical class 0.000 claims description 9
- 239000002904 solvent Substances 0.000 claims description 7
- 238000007605 air drying Methods 0.000 claims description 5
- 238000010790 dilution Methods 0.000 claims description 4
- 239000012895 dilution Substances 0.000 claims description 4
- 230000000694 effects Effects 0.000 description 10
- 239000002537 cosmetic Substances 0.000 description 4
- 239000000839 emulsion Substances 0.000 description 4
- 239000004615 ingredient Substances 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 3
- 241000700605 Viruses Species 0.000 description 3
- 239000002131 composite material Substances 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 108090000765 processed proteins & peptides Proteins 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 description 2
- 230000001476 alcoholic effect Effects 0.000 description 2
- 230000027455 binding Effects 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 238000001879 gelation Methods 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 230000036571 hydration Effects 0.000 description 2
- 238000006703 hydration reaction Methods 0.000 description 2
- 239000001866 hydroxypropyl methyl cellulose Substances 0.000 description 2
- 229920003088 hydroxypropyl methyl cellulose Polymers 0.000 description 2
- UFVKGYZPFZQRLF-UHFFFAOYSA-N hydroxypropyl methyl cellulose Chemical compound OC1C(O)C(OC)OC(CO)C1OC1C(O)C(O)C(OC2C(C(O)C(OC3C(C(O)C(O)C(CO)O3)O)C(CO)O2)O)C(CO)O1 UFVKGYZPFZQRLF-UHFFFAOYSA-N 0.000 description 2
- 235000010979 hydroxypropyl methyl cellulose Nutrition 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 239000003109 Disodium ethylene diamine tetraacetate Substances 0.000 description 1
- XEFQLINVKFYRCS-UHFFFAOYSA-N Triclosan Chemical compound OC1=CC(Cl)=CC=C1OC1=CC=C(Cl)C=C1Cl XEFQLINVKFYRCS-UHFFFAOYSA-N 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000000172 allergic effect Effects 0.000 description 1
- 208000010668 atopic eczema Diseases 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 239000006184 cosolvent Substances 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 235000019301 disodium ethylene diamine tetraacetate Nutrition 0.000 description 1
- 239000008393 encapsulating agent Substances 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 239000010419 fine particle Substances 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
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- 239000003906 humectant Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 230000000622 irritating effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000012567 medical material Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
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- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 230000000979 retarding effect Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000002562 thickening agent Substances 0.000 description 1
- 229960003500 triclosan Drugs 0.000 description 1
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/04—Dispersions; Emulsions
- A61K8/042—Gels
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/10—Washing or bathing preparations
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Dermatology (AREA)
- Dispersion Chemistry (AREA)
- Chemical & Material Sciences (AREA)
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- Medicinal Preparation (AREA)
- Cosmetics (AREA)
Abstract
The invention relates to a preparation method of an alcohol-fibroin gel washing-free disinfectant. At present, no disposable disinfectant which is safe to use, convenient to smear on hands and capable of moistening skin does not exist. The invention is characterized in that: the preparation method comprises the following steps: uniformly mixing 100ml of medical alcohol solution, 5-10ml of silk fibroin solution and 0-2ml of micromolecular sericin solution to prepare alcohol-fibroin gel washing-free disinfectant; the operation of homogeneous mixing is as follows: adding the silk fibroin solution into a container, then slowly adding the small molecular sericin solution, stirring for 2-3 minutes, then quickly adding the medical alcohol solution, stirring and mixing for 20 seconds-1 minute, and finishing the mixing. The alcohol-fibroin gel washing-free disinfectant is safe to human bodies, convenient to use and capable of moistening skin.
Description
Technical Field
The invention relates to a preparation method of a hand-use no-clean disinfectant, in particular to a preparation method of an alcohol-fibroin gel no-clean disinfectant.
Background
The hands usually contact the most bacteria and viruses in daily life, the bacteria and viruses can easily enter the body through food contacted by the hands or the hands touch the face to cause infection, and the bacteria and viruses infected on the hands can be further transmitted to other people through contacting common objects. Therefore, frequent hand washing is necessary to keep the hands clean, which is especially important before catering and when going home.
The hand is generally washed by the hand sanitizer and then is washed by water. But spraying a no-clean disinfectant on the hands is also a method in the case of no water around the body. The no-clean disinfectant with good sterilizing effect and safety has alcohol with the concentration of about 75 percent, but the alcohol also has the defect that the skin is irritated, and the skin on hands is easy to be rough and allergic after the no-clean disinfectant is frequently used. Therefore, some alcohol added with other skin care ingredients is taken as a no-clean disinfectant on the market, and various ingredients are often added into the no-clean disinfectant, and some added ingredients can be used for skin care, but are unsafe to be taken by the careless entrance. For example, in chinese patent publication No. CN101912358A, publication No. 2010, No. 12 and 15, triclosan is used as the main active ingredient, and ethanol, propylene glycol, glycerol, hydroxypropyl methylcellulose (HPMC), disodium ethylenediaminetetraacetate (EDTA-2Na), etc. are used as auxiliary ingredients, so as to provide a hand-washing-free disinfectant having an irritant effect on skin. Also, as disclosed in the chinese patent publication No. CN106265436A, publication No. 01/04 in 2017, a formulation of a leave-on disinfectant with skin care effect and a preparation method thereof are disclosed, wherein the leave-on disinfectant comprises a thickener, a disinfectant, a cosolvent, an encapsulating agent, a neutralizer, a humectant, a skin care agent, an essence and deionized water, and has complex components and poor skin care effect.
Disclosure of Invention
The invention aims to overcome the defects in the prior art and provides a preparation method of the alcohol-fibroin gel no-clean disinfectant which is safe to human bodies, convenient to use and capable of moistening skins.
The technical scheme adopted by the invention for solving the problems is as follows: the preparation method of the alcohol-fibroin gel washing-free disinfectant is characterized in that: the preparation method comprises the following steps: uniformly mixing 100ml of medical alcohol solution, 5-10ml of silk fibroin solution and 0-2ml of micromolecular sericin solution to prepare alcohol-fibroin gel washing-free disinfectant; the operation of homogeneous mixing is as follows: adding the silk fibroin solution into a container, then quickly adding the medical alcohol solution, stirring and mixing for 1-3 minutes, then slowly adding the small-molecular sericin solution, stirring for 2-3 minutes, and finishing mixing.
Preferably, the volume concentration of the medical alcohol solution is 30-70%.
Preferably, the concentration of the silk fibroin solution of the present invention is 0.5-8% w/v.
Preferably, the concentration of the small-molecule sericin solution is 0.5-2% w/v.
Preferably, when the alcohol concentration in the alcohol-fibroin gel no-clean disinfectant needs to be increased, the alcohol with the concentration of 90-100% is continuously added into the prepared alcohol-fibroin gel no-clean disinfectant to adjust the alcohol-fibroin gel no-clean disinfectant to the required alcohol concentration, and the mixture is stirred strongly for 0.5-1 minute by a stirrer with the stirring speed of 400-. Although the alcohol-fibroin gel washing-free disinfectant prepared finally is not in a gel state any more after the treatment, but becomes a semitransparent thin emulsion state. The alcohol-fibroin gel washing-free disinfectant in the emulsion state has little influence on the skin care effect.
Preferably, the rotation speed of the stirring in the invention is 100-200 rpm.
Preferably, the operation of uniformly mixing according to the present invention is performed at a temperature of 0 to 30 ℃.
Preferably, the alcohol-fibroin gel washing-free disinfectant consists of 100ml of medical alcohol solution, 5-10ml of silk fibroin solution and 0-2ml of small molecular sericin solution.
Preferably, the preparation method of the silk fibroin solution provided by the invention comprises the following steps: applying 0.5% (w/v) Na on cocoon shell2CO2Degumming at bath ratio of 1:200 for 30min each time, washing with clear water, repeating the steps, and drying to obtain silk fibroin; with 50% (w/v) of CaC12The silk fibroin fiber is dissolved in the water solution at the temperature of 98 ℃ and the bath ratio of the silk fibroin fiber g to the CaC12The water solution ml is 1:25, and the time is 10 minutes; and filling the solution obtained after dissolution into a dialysis bag, intercepting the solution with the molecular weight of 12000-14000, dialyzing in running water for 3d, and adding water for dilution to obtain the silk fibroin solution.
Preferably, the preparation method of the silk fibroin solution provided by the invention comprises the following steps: applying 0.5% (w/v) Na on cocoon shell2CO2Degumming at bath ratio of 1:200 for 30min each time, washing with clear water, repeating the steps, and drying to obtain silk fibroin; dissolving the silk fibroin fibers by using a ternary solvent, wherein the bath ratio of the silk fibroin fibers to the ternary solution ml is 1:15, the dissolving temperature is 80 ℃, and the time is 10 minutes; and filling the solution obtained after dissolution into a dialysis bag, intercepting the molecular weight of 12000-14000, dialyzing in running water for 3d, and air-drying and concentrating to obtain the silk fibroin solution.
Preferably, the proportion of the ternary solvent is n (CaCl)2)∶n(H2O)∶n(C2H5OH)＝1∶8∶2。
Compared with the prior art, the invention has the following advantages and effects: fibroin protein and sericin extracted from silk are collectively called as fibroin protein, are safe to human bodies, and have application in the aspects of food, medical materials and cosmetics. The silk fibroin and the sericin have various skin care effects of nourishing skin, cleaning, resisting oxidation, moisturizing and the like, and are ideal skin care additive components capable of being used as a washing-free disinfectant.
The alcohol-fibroin gel washing-free disinfectant with alcohol and fibroin in the invention does not contain other components except medical alcohol solution, fibroin solution and micromolecular sericin solution, and is very safe to use. The alcohol-fibroin gel washing-free disinfectant has a flowing gelatinous form, and is convenient to coat on hands; the volatilization of alcohol components can be delayed, and the sterilization effect is enhanced; in addition, it can moisten skin.
The principle of the invention is as follows:
the fibroin solution can generate structural change when meeting alcohol, and weak bonds such as hydrogen bonds and the like are generated between fibroin molecules for combination, because the dehydration effect of the alcohol changes the hydration state of the surfaces of the fibroin molecules in the solution. At alcohol concentrations below 70%, the weak bond binding and hydration state of fibroin reaches an equilibrium, forming a gel. Under the condition that the alcohol concentration is higher than 70%, the weak bond binding action of the fibroin molecules is enhanced, and the molecules are agglomerated and then separated out from the solution into flocculent or solid particulate. Therefore, the concentration of the alcohol solution added in the invention is 30-70%.
If the fibroin solution is added into the alcoholic solution, the fibroin which is firstly contacted with a large amount of alcoholic solution can be firstly gelled and agglomerated, and is not easy to mix uniformly. Therefore, the medical alcohol solution is quickly added into the fibroin solution and stirred and mixed.
When high concentration alcohol is added into the formed alcohol-fibroin gel, the fibroin still has the tendency of being precipitated from the solution, but because the fibroin is combined with weak bonds to form gel at the moment, the gel is in an irreversible state, and the precipitation of the fibroin is more precipitated in the state of gel particles. When the stirring is performed with strong force, the fine particles of the precipitates are relatively uniform and are not easily precipitated. Therefore, when the alcohol concentration of the alcohol-fibroin gel disposable disinfectant is adjusted to be more than 70%, high-concentration alcohol needs to be added on the basis of the prepared alcohol-fibroin gel disposable disinfectant.
Drawings
In order to more clearly illustrate the embodiments and/or technical solutions of the present invention, the drawings used in the description of the embodiments and/or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.
Fig. 1 is a schematic diagram of the effect of the fibroin solution of the present invention in retarding the volatilization of alcohol components.
Detailed Description
The present invention will be described in further detail below by way of examples with reference to the accompanying drawings, which are illustrative of the present invention and are not to be construed as limiting the present invention.
Example 1.
The preparation method of the alcohol-fibroin gel washing-free disinfectant in the embodiment is as follows.
Applying 0.5% (w/v) Na on cocoon shell2CO2Degumming at bath ratio of 1:200 for 30min each time, washing with clear water, repeating the steps, and drying to obtain the silk fibroin fiber. With 50% (w/v) of CaC12The silk fibroin fiber is dissolved in the water solution at 98 deg.C, and the bath ratio (silk fibroin g: CaC 1)2Aqueous ml) was 1:25 for 10 minutes. And filling the solution obtained after dissolution into a dialysis bag (with the molecular weight cut-off of 12000-14000), dialyzing in running water for 3 days, and adding water for dilution to obtain the silk fibroin solution with the concentration of 1% (w/v).
50ml of silk fibroin solution with the concentration of 1% (w/v) is added into a container, then 1L of medical alcohol with the volume percentage concentration of 75% is rapidly added, and the mixture is immediately stirred for 0.5 minute at the stirring speed of 150 revolutions per minute to obtain the thin alcohol-fibroin gel washing-free disinfectant.
Example 2.
The preparation method of the alcohol-fibroin gel washing-free disinfectant in the embodiment is as follows.
Applying 0.5% (w/v) Na on cocoon shell2CO2Degumming at bath ratio of 1:200 for 30min each time, washing with clear water, repeating the steps, and drying to obtain the silk fibroin fiber. Dissolving fibroin fiber with ternary solvent at a ratio of [ n (Ca _ Cl) ]2)∶ n(H2O)∶n(C2H5OH)＝1∶8∶2]The bath ratio (silk fibroin fiber g: ternary solution ml) is 1:15, the dissolving temperature is 80 ℃, and the time is 10 minutes. And filling the solution obtained after dissolution into a dialysis bag (with the molecular weight cut-off of 12000-14000), dialyzing in running water for 3 days, and air-drying and concentrating to obtain a 5% (w/v) silk fibroin solution.
The preparation method of the small-molecule sericin solution can be seen in patent application No. 201810298137.8 with the patent name of "a preparation method of a water-soluble silk fibroin sericin composite cosmetic product", and the sericin peptide solution in patent application No. 201810298137.8 is the small-molecule sericin solution and can be applied to the embodiment.
Adding 50ml of 5% (w/v) silk fibroin solution into a container, then slowly adding 2ml of 1% (w/v) micromolecular sericin solution while stirring, stirring for 3 minutes at the stirring speed of 150 revolutions per minute, then quickly adding 1L of medical alcohol with the volume percentage concentration of 65%, and immediately stirring for 20 seconds to obtain the alcohol-silk protein gel washing-free disinfectant with slightly high consistency.
Example 3.
The preparation method of the alcohol-fibroin gel washing-free disinfectant in the embodiment is as follows.
Applying 0.5% (w/v) Na on cocoon shell2CO2Degumming at bath ratio of 1:200 for 30min each time, washing with clear water, repeating the steps, and drying to obtain the silk fibroin fiber. Dissolving fibroin fiber with ternary solvent at a ratio of [ n (Ca _ Cl) ]2)∶ n(H2O)∶n(C2H5OH)＝1∶8∶2]The bath ratio (silk fibroin fiber g: ternary solution ml is 1:15), the dissolving temperature is 80 ℃, and the time is 10 minutes. And filling the solution obtained after dissolution into a dialysis bag (with the molecular weight cut-off of 12000-14000), dialyzing in running water for 3 days, and air-drying and concentrating to obtain 8% (w/v) silk fibroin solution.
The preparation method of the small-molecule sericin solution can be seen in patent application No. 201810298137.8 with the patent name of "a preparation method of a water-soluble silk fibroin sericin composite cosmetic product", and the sericin peptide solution in patent application No. 201810298137.8 is the small-molecule sericin solution and can be applied to the embodiment.
Adding 60ml of 8% (w/v) silk fibroin solution into a container, then slowly adding 1.5ml of 0.8% (w/v) micromolecular sericin solution while stirring for 3 minutes at the stirring speed of 200 r/min, then quickly adding 1L of medical alcohol with the volume percentage concentration of 70%, and immediately stirring for 1 minute to obtain the alcohol-fibroin gel washing-free disinfectant with higher consistency.
Example 4.
The preparation method of the alcohol-fibroin gel washing-free disinfectant in the embodiment is as follows: uniformly mixing 100ml of medical alcohol solution, 5-10ml of silk fibroin solution and 0-2ml of micromolecular sericin solution to prepare alcohol-fibroin gel washing-free disinfectant; the operation of homogeneous mixing is as follows: adding the silk fibroin solution into a container, adding 70% of the silk fibroin solution, slowly adding the micromolecular sericin solution, stirring for 2-3 minutes at the rotation speed of 150-. The whole operation of homogeneous mixing is carried out at a temperature of 0 to 30 ℃.
The volume percentage concentration of the medical alcohol solution in the embodiment can be 30-70%, the concentration of the silk fibroin solution can be 0.5-8% w/v, and the concentration of the small molecule sericin solution can be 0.5-2% w/v.
The alcohol-fibroin gel washing-free disinfectant in the embodiment preferably only comprises 100ml of medical alcohol solution, 5-10ml of silk fibroin solution and 0-2ml of small molecular sericin solution.
When the alcohol concentration in the alcohol-fibroin gel no-clean disinfectant needs to be increased, continuously adding 90-100% alcohol into the prepared alcohol-fibroin gel no-clean disinfectant to adjust the alcohol-fibroin gel no-clean disinfectant to the required alcohol concentration, and strongly stirring for 0.5-1 minute by using a 400-ion 1000 r/min stirring machine to obtain the finished product. Although the alcohol-fibroin gel washing-free disinfectant prepared finally is not in a gel state any more after the treatment, but becomes a semitransparent thin emulsion state. The alcohol-fibroin gel washing-free disinfectant in the emulsion state has little influence on the skin care effect.
The preparation method of the silk fibroin solution in the embodiment can be as follows: applying 0.5% (w/v) Na on cocoon shell2CO2Degumming at bath ratio of 1:200 for 30min each time, washing with clear water, repeating the steps, and drying to obtain silk fibroin; with 50% (w/v) of CaC12The silk fibroin fiber is dissolved in the water solution at the temperature of 98 ℃ and the bath ratio of the silk fibroin fiber g to the CaC12The water solution ml is 1:25, and the time is 10 minutes; and filling the solution obtained after dissolution into a dialysis bag, intercepting the solution with the molecular weight of 12000-14000, dialyzing in running water for 3d, and adding water for dilution to obtain the silk fibroin solution. The preparation method of the silk fibroin solution can also be as follows: applying 0.5% (w/v) Na on cocoon shell2CO2Degumming at bath ratio of 1:200 for 30min each time, washing with clear water, repeating the steps, and drying to obtain silk fibroin; dissolving silk fibroin fiber with ternary solvent at a ratio of n (CaCl)2)∶n(H2O)∶ n(C2H5OH) is 1: 8: 2, the bath ratio is that the silk fibroin g is added into the ternary solution ml is 1:15, the dissolving temperature is 80 ℃, and the time is 10 minutes; and filling the solution obtained after dissolution into a dialysis bag, intercepting the molecular weight of 12000-14000, dialyzing in running water for 3d, and air-drying and concentrating to obtain the silk fibroin solution.
The preparation method of the small-molecule sericin solution can be seen in patent application No. 201810298137.8 with the patent name of "a preparation method of a water-soluble silk fibroin sericin composite cosmetic product", and the sericin peptide solution in patent application No. 201810298137.8 is the small-molecule sericin solution and can be applied to the embodiment.
As shown in FIG. 1, the evaporation rates of alcohol and the alcohol-fibroin gel disposable disinfectant of the present invention were compared, and "series 1" in FIG. 1 is the initial solution weight and "series 2" is the solution weight after two hours. Bar 1 in fig. 1 represents 5.5ml of alcohol with a concentration of 70% by volume; bar 2 represents 5ml of 70% alcohol by volume + 0.25ml of 4.8% w/v silk fibroin solution; bar 3 represents 5ml of 70% alcohol by volume + 0.4ml of 4.8% w/v silk fibroin solution; bar 4 represents 5ml of 70% alcohol by volume + 0.5ml of silk fibroin solution at 4.8% w/v. After the 4 solutions were added into glass dishes with a diameter of 3.7mm, the glass dishes were placed at a temperature of 20 ℃, RH 40% relative humidity, and volatilized indoors with a fan, comparative experiments were performed, and then the experimental results were prepared as shown in FIG. 1, and it can be seen from FIG. 1 that the fibroin solution can delay the volatilization of alcohol components.
The following experiment was performed by mixing 0.5ml of silk fibroin solutions with different concentrations with 10ml of alcohol with a volume percentage concentration of 60%, to obtain the time required for gelation after mixing the alcohol with the silk fibroin solutions with different concentrations, as shown in the following table.
| Concentration% (w/v) of silk fibroin | 4.8 | 4 | 3 | 2 | 1 | 0.5 |
| Gel time (minutes) | 5 | 8 | 15 | 30 | 60 | 90 |
| Fluidity of gel | Flow of | Flow of | Flow of | Flow of | Flow of | Flow of |
From the above table, it can be seen that the concentration of the fibroin solution can be mixed with alcohol in a wide range to form a gel having fluidity, and the lower the concentration of the fibroin solution, the longer the time required for the gel. The lower the concentration of the fibroin solution, the thinner the gel formed, and the concentration of the fibroin solution can be selected within a certain range according to requirements in production.
An experiment was performed using 0.5ml of 4.8% (w/v) silk fibroin solution mixed with 10ml of different alcohol concentrations to obtain the time required for gelation after mixing the silk fibroin solution with the different alcohol concentrations, as shown in the following table.
| Alcohol concentration% | 30 | 40 | 50 | 60 | 70 |
| Gel time (minutes) | 120 | 30 | 15 | 5 | 3 |
| Fluidity of gel | Flow of | Flow of | Flow of | Flow of | Flow of |
As can be seen from the above table, the alcohol with concentration range of 30-70% can be mixed with the fibroin solution to form a gel with fluidity. Different alcohol concentrations may be selected according to the use requirements.
The alcohol-fibroin gel washing-free disinfectant disclosed by the invention is not added with other components except for the medical alcohol solution, the fibroin solution and the micromolecular sericin solution, is very safe to use, and can moisten the skin. Has flowing gel form, is convenient for smearing on hands, can delay the volatilization of alcohol components, and enhances the sterilization effect.
Although the present invention has been described with reference to the above embodiments, it should be understood that the scope of the present invention is not limited thereto, and that various changes and modifications can be made by those skilled in the art without departing from the spirit and scope of the present invention.
Claims (10)
1. A method for preparing alcohol-fibroin gel washing-free disinfectant is characterized by comprising the following steps: the preparation method comprises the following steps: uniformly mixing 100ml of medical alcohol solution, 5-10ml of silk fibroin solution and 0-2ml of micromolecular sericin solution to prepare alcohol-fibroin gel washing-free disinfectant; the operation of homogeneous mixing is as follows: adding the silk fibroin solution into a container, then slowly adding the small molecular sericin solution, stirring for 2-3 minutes, then quickly adding the medical alcohol solution, stirring and mixing for 20 seconds-1 minute, and finishing the mixing.
2. The method for preparing the alcohol-fibroin gel washing-free disinfectant according to claim 1, characterized in that: the volume concentration of the medical alcohol solution is 30-70%.
3. The method for preparing the alcohol-fibroin gel washing-free disinfectant according to claim 1, characterized in that: the concentration of the silk fibroin solution is 0.5-8% w/v, and/or the concentration of the small molecule sericin solution is 0.5-2% w/v.
4. The method for preparing the alcohol-fibroin gel washing-free disinfectant according to claim 1, characterized in that: when the alcohol concentration in the alcohol-fibroin gel no-clean disinfectant needs to be increased, continuously adding 90-100% alcohol into the prepared alcohol-fibroin gel no-clean disinfectant to adjust the alcohol-fibroin gel no-clean disinfectant to the required alcohol concentration, and strongly stirring for 0.5-1 minute by using a 400-ion 1000 r/min stirring machine to obtain the finished product.
5. The method for preparing the alcohol-fibroin gel washing-free disinfectant according to claim 1, characterized in that: the rotation speed of the stirring is 100-200 rpm.
6. The method for preparing the alcohol-fibroin gel washing-free disinfectant according to claim 1, characterized in that: the operation of said homogeneous mixing is carried out at a temperature of 0-30 ℃.
7. The method for preparing the alcohol-fibroin gel washing-free disinfectant according to claim 1, characterized in that: the alcohol-fibroin gel washing-free disinfectant consists of 100ml of medical alcohol solution, 5-10ml of silk fibroin solution and 0-2ml of micromolecular sericin solution.
8. The method for preparing the alcohol-fibroin gel washing-free disinfectant according to claim 1, characterized in that: the preparation method of the silk fibroin solution comprises the following steps: applying 0.5% (w/v) Na on cocoon shell2CO2Degumming at bath ratio of 1:200 for 30min each time, washing with clear water, repeating the steps, and drying to obtain silk fibroin; with 50% (w/v) of CaC12The silk fibroin fiber is dissolved in the water solution at the temperature of 98 ℃ and the bath ratio of the silk fibroin fiber g to the CaC12Aqueous ml =1:25 for 10 minutes; and filling the solution obtained after dissolution into a dialysis bag, intercepting the solution with the molecular weight of 12000-14000, dialyzing in running water for 3d, and adding water for dilution to obtain the silk fibroin solution.
9. The method for preparing the alcohol-fibroin gel washing-free disinfectant according to claim 1, characterized in that: the preparation method of the silk fibroin solution comprises the following steps: applying 0.5% (w/v) Na on cocoon shell2CO2Degumming at bath ratio of 1:200 for 30min each time, washing with clear water, repeating the steps, and drying to obtain silk fibroin; dissolving the silk fibroin fibers by using a ternary solvent, wherein the bath ratio is that the silk fibroin fibers are g to the ternary solution ml =1:15, the dissolving temperature is 80 ℃, and the time is 10 minutes; and filling the solution obtained after dissolution into a dialysis bag, intercepting the molecular weight of 12000-14000, dialyzing in running water for 3d, and air-drying and concentrating to obtain the silk fibroin solution.
10. The method for preparing the alcohol-fibroin gel washing-free disinfectant according to claim 9, wherein: the proportion of the ternary solvent is n (CaCl)2）∶n（H2O）∶n（C2H5OH）=1∶8∶2。
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