CN1113515A - Bifidobacterium microcapsules and their manufacture - Google Patents
Bifidobacterium microcapsules and their manufacture Download PDFInfo
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- CN1113515A CN1113515A CN 95102072 CN95102072A CN1113515A CN 1113515 A CN1113515 A CN 1113515A CN 95102072 CN95102072 CN 95102072 CN 95102072 A CN95102072 A CN 95102072A CN 1113515 A CN1113515 A CN 1113515A
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Abstract
The Bifidodacterium microcapsule (0.5-1mm in dimeter) is prepared with two kinds of edible gum as raw material of capsule, plasticizer, living Bifidodacterium bacteria and solidifying agent and contains the number of living Bifidodacterium bacteria of greater than 10 to the power 9/g. It features low cost, simple process and keeping the living bacteria alive for a period that is elongated by 50-190 days.
Description
The present invention relates to a kind of microcapsule and manufacture method thereof that contains bifidus bacillus (Bifidobacteria) viable bacteria.
Kind surplus the product of bifidus bacillus has reached 70 both at home and abroad, they comprise sour milk, buttermilk, yogurt fat, milk powder, quick-frozen dessert, tablet and lozenge etc.But the preservation period of bifidobacteria viable bacteria is all very short, shorter in liquid product, and general low temperature (4-10 ℃) is preserved 3-5 days viable counts down and promptly is lower than 10
5Individual/ml, so the viable bacteria preservation period that how to prolong bifidus bacillus in the liquid product is to fall over each other the difficult point that solves both at home and abroad, adopts microcapsule embedded method not to be reported.
Purpose of the present invention, adopt microcapsulary exactly, bifidus bacillus is wrapped in the capsule material, isolate with acid and oxygen in the environment, add and agent in the microcapsule, in and the acid that in preservation term, produced of bifidus bacillus, to reach the purpose that in bifido-containing product especially liquid product, keeps higher viable count.Preparation microcapsule material therefor all has no side effect to human body.
The present invention includes following steps
Microcapsule prescriptions (consumption in the 100ml mixed glue solution): the capsule material is made up of edible seaweed acid sodium and gelatin, and the ratio of the two is 1-3: the 1(weight ratio), the two (sum) add-on is 2-5g; Lime carbonate (CaCO
3), sorbyl alcohol or glycerine is auxilliary material, wherein the add-on of lime carbonate is 0.2-0.5g, the add-on of sorbyl alcohol or glycerine is 2-5g; Bifidus bacillus (can at each kind of bifidus bacillus of human intestinal field planting) viable bacteria 10
11-10
13Individual, the adding mode is separated the wet bacterium mud that makes, about 2-6g for adding with the 100-300ml bacterial culture fluid; Distilled water 83.5-93.3ml.Calcium chloride (CaCl
2) be solidifying agent, concentration of aqueous solution 0.1-0.3mol/l, consumption are 400-600ml.
Microcapsule preparation technology:
1. the cultivation of bifidus bacillus
Bacterial classification through 2-3 switching activation, is seeded in the liquid nutrient medium in solid medium or liquid nutrient medium then, 37 ± 1 ℃ constant temperature culture 18-24 hour, when viable count reaches 10
8-10
10Individual/as to finish during ml to cultivate cooling, aseptic condition following 4000 rev/mins (or high speed centrifugations) 15-20 minute, centrifugal collection thalline; abandoning supernatant adds stroke-physiological saline solution and suspends, and is centrifugal, washing thalline 2 times; obtain wet bacterium mud, charge into aseptic nitrogen protection, standby.
2. the preparation of capsule material solution and sterilization
Get the distilled water of desiring compounding rubber alloy liquid total amount 80%, add the jelly powder that weighs up, stir heating down (about 80 ℃, down together) to dissolving fully, under constantly stirring, add sodium alginate then in batches, continue heated and stirred, add building paper wrapping rim of a cup with the interlayer gauze to becoming transparent glue, sterilized 15-20 minute down in 8-15 pound pressure, standby.
3. the preparation of auxiliary material and solidifying agent and sterilization (purity is analytical pure)
CaCO
3: contain the 0.2-0.5g amount by per hundred milliliters of mixed glue solutions and take by weighing CaCO
3Powder is wrapped with building paper.
Sorbyl alcohol: the 60g sorbyl alcohol adds the 50ml dissolved in distilled water, is settled to 100ml then, gets 60% aqueous solution.
Glycerine: 98% stoste.
CaCl
2: be made into 0.1-0.3mol/l solution with distilled water.
More than sterilized 15-20 minute CaCO in the 8-15 pound with preceding
3The paper bag oven dry, standby.
4. microcapsule preparation
Press formula ratio adds sterilization respectively in aseptic sack material solution CaCO
3, sorbyl alcohol or glycerine, under aseptic nitrogen protection, stir evenly, add bacterium mud then, mend sterile distilled water to final volume, mixed glue solution.After fully stirring evenly, draw mixed glue solution,, spray into the CaCl that places on the magnetic stirring apparatus and stirring with 5 or No. 6 syringe needles with syringe
2In the solution (aseptic nitrogen protection).After having sprayed, stir at a slow speed down and solidified 5-30 minute,, promptly get the microcapsule that wet with sterilized water washing 3 times.Capsule diameter is 0.5-1mm, viable count 10
9-10
11Individual/g.
The advantage of microcapsule:
1. can prolong the viable bacteria preservation period of bifidus bacillus greatly, the preservation effect the best in liquid product.As, with the Radix Dauci Sativae juice of bifidobacterium fermentation, initial viable count is 10
9Individual/ml, to preserve 7-10 days down for 4 ℃, 16-20 ℃ of following 3-5 days viable count is with regard to＜10
5Individual/ml, preservation period then prolongs greatly behind the interpolation bifid bacterium microcapsule, and preservation period is with viable count〉10
5Individual/ml exceeds, embedding bifidus longum bb (B.longum), and its preservation effect sees Table 1:
Table 1: the preservation effect table of bifidus bacillus in the microcapsule
| Storage temperature | Bifidobacterium fermentation juice pH4.2 | Nonfermented juice pH6.4 |
| 4℃ 16－20℃ | 90-120 days 30-60 days | 150-220 days 60-100 days |
2. the processing units requirement is low, technology is simple, cost is low.All devices does not have particular requirement except that granulating equipment, all the course of processing is all carried out at normal temperatures and pressures, and the cost of the wet microcapsule of every 100g about 2.80 yuan (calculating in February nineteen ninety-five) is if by increasing only 0.7 yuan of cost in the 10% add-on adding 250ml beverage.
3. microcapsule diameter 0.5-1mm only adds no tangible bad mouthfeel in the liquid product, then can promote product appearance and kind if making colored capsule is suspended in the transparent liquid goods, the enhancing product competitiveness on the contrary.
These microcapsule also can add in the food such as yogurt, ice cream, dessert, candy.
The present invention has following accompanying drawing:
Fig. 1. bifid bacterium microcapsule preparation technology schema
Description of drawings:
1. capsule material solution preparation whole process (2) needs heating, and about 80 ℃ of temperature are noted fully stirring.
2. aseptic sack material solution will fully be mixed thoroughly after adding auxiliary material, bacterium mud, and wants aseptic nitrogen protection.
3. granulation process (4) carries out on magnetic stirring apparatus, aseptic nitrogen protection.
4. all operating process needs to carry out under aseptic condition.
Most preferred embodiment of the present invention is as follows:
1. product embodiments
Prepare bifid bacterium microcapsule with the 100ml mixed glue solution.Contain in the used mixed glue solution: sodium alginate and gelatin 2-5g, sodium alginate: gelatin=1-3: 1, lime carbonate 0.2-0.5g, glycerine 2-5g, bifidus longum bb (B.longum) viable bacteria 10
11-10
13Individual.
Prepare bifid bacterium microcapsule with the 100ml mixed glue solution.Contain in the used mixed glue solution: sodium alginate and gelatin 2-5g, sodium alginate: gelatin=1-3: 1, lime carbonate 0.2-0.5g, glycerine 2-5g, bifidus longum bb (B.longum) viable bacteria 10
11-10
13Individual.
2. process implementing example
If desiring compounding rubber alloy liquid cumulative volume is 100ml.
A. the clay bacterial classification that is equipped with of wet bacterium activates through 2-3 switching in solid medium or liquid nutrient medium, be seeded in then in the 100-300ml liquid nutrient medium, 37 ± 1 ℃ constant temperature culture 18-24 hour, when viable count reaches 10
8-10
10Individual/as to finish during ml to cultivate, cooling, aseptic condition following 4000 rev/mins (or at a high speed) centrifugal 15-20 minute, centrifugal collection thalline; abandoning supernatant adds stroke-physiological saline solution and suspends, and is centrifugal, washing thalline 2 times; obtain wet bacterium mud, charge into aseptic nitrogen protection, standby.
B. the preparation of capsule material solution and sterilization take by weighing in the 1-2g gelatin adding 80ml distilled water, and 80 ℃ of following heating for dissolving add the 2-3g sodium alginate under stirring in batches, stir and be heated to dissolving, the interlayer gauze adds building paper wrapping rim of a cup, and 8-15 pound autoclaving 15-20 minute is standby.
C. the preparation of auxiliary material and solidifying agent and sterilization calcium carbonate powders 0.2-0.5g wrap with building paper; The 60g sorbyl alcohol with the 50ml dissolved in distilled water after adding distil water be settled to 100ml, (glycerine is 98% stoste); With distilled water calcium chloride is made into 0.1-0.3mol/l solution, consumption is 400-600ml.More than in down sterilization 15-20 minute of 8-15 pound, standby.
D. in the preparation aseptic sack material solution of microcapsule, feed aseptic nitrogen, add the CaCO of sterilization
3, add 60% sorbyl alcohol or 98% glycerine 2-4ml, stir evenly, add bacterium mud then, mend sterile distilled water to 100ml, mixed glue solution.After fully stirring evenly, draw mixed glue solution,, spray into the 400-600mlCaCl that places on the magnetic stirring apparatus and stirring with 5 or No. 6 syringe needles with syringe
2In the solution (aseptic nitrogen protection).After having sprayed, stir at a slow speed down and solidified 5-30 minute,, promptly get the microcapsule that wet with sterilized water washing 3 times.Capsule diameter is 0.5-1mm, viable count 10
9-10
11Individual/g.The preparation whole process needs aseptic technique.
Claims (3)
1, a kind of bifid bacterium microcapsule is characterized in that containing sodium alginate, gelatin, lime carbonate, sorbyl alcohol or glycerine and bifidus bacillus (Bifidobacteria) viable bacteria.
2, bifid bacterium microcapsule according to claim 1, it is characterized in that making in per hundred milliliters of the used mixed glue solutions of microcapsule and contain: sodium alginate, gelatin (sum of the two) 2-5g, sodium alginate is 1-3 with the ratio of gelatin: 1, lime carbonate 0.2-0.5g, sorbyl alcohol or glycerine 2-5g, bifidobacteria viable bacteria 10
11-10
13Individual.
3, a kind of preparation technology of bifid bacterium microcapsule, it comprises bifidobacterium species activation, liquid culture, centrifugation, washing, wet bacterium mud and makes the microcapsule that contain bifidobacteria viable bacteria, it is characterized in that:
A. the proportioning of capsule material solution and preparation: the capsule material by edible seaweed acid sodium and gelatin by 1-3: 1 ratio is prepared, and the two (sum) be 2-5g in per hundred milliliters of mixed glue solutions.Take by weighing jelly powder by formula ratio, stir and add down in the distilled water of desiring compounding rubber alloy liquid cumulative volume 80%, heating is (about 80 ℃, be stirred to dissolving fully down together), stir then and add sodium alginate down in batches, continue to stir, be heated to and become transparent glue, in sterilization 15-20 minute down of 8-15 pound pressure, standby.
B. the preparation of auxiliary material and solidifying agent and consumption: the add-on of calcium carbonate powders (per hundred milliliters of mixed glue solutions, down with) is 0.2-0.5g; With distilled water sorbyl alcohol is made into 60% solution, glycerine is 98% stoste, and the add-on of sorbyl alcohol or glycerine is 2-5g; With distilled water calcium chloride is made into 0.1-0.3mol/l solution, consumption is 400-600ml.More than sterilized 15-20 minute down in the 8-15 pound with preceding, standby.
C. microcapsule preparation: add sterilization lime carbonate, Sorbitol Solution USP or glycerine respectively in the sterilization capsule material solution; stir evenly the back under the aseptic nitrogen protection and add bacterium mud; mend sterile distilled water to desiring compounding rubber alloy liquid cumulative volume, after stirring evenly, draw mixed glue solution with syringe; with 5 or No. 6 syringe needles; spray into the calcium chloride solution (aseptic nitrogen protection) that places on the magnetic stirring apparatus and stirring, after having sprayed, stir at a slow speed and solidified 5-30 minute; inclining calcium chloride solution, promptly gets product 3 times with aseptic washing capsule.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN95102072A CN1056877C (en) | 1995-03-06 | 1995-03-06 | Bifidobacterium microcapsules and their manufacture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN95102072A CN1056877C (en) | 1995-03-06 | 1995-03-06 | Bifidobacterium microcapsules and their manufacture |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1113515A true CN1113515A (en) | 1995-12-20 |
| CN1056877C CN1056877C (en) | 2000-09-27 |
Family
ID=5074160
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN95102072A Expired - Fee Related CN1056877C (en) | 1995-03-06 | 1995-03-06 | Bifidobacterium microcapsules and their manufacture |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1063051C (en) * | 1998-03-27 | 2001-03-14 | 内蒙古自治区畜牧科学院 | Composite live bifidobacterium prepn |
| US6841181B2 (en) | 1999-12-22 | 2005-01-11 | Nutrinova Nutrition Specialties & Food Ingredients Gmbh | Encapsulated multifunctional biologically active food component, process for its production and its use |
| CN107279586A (en) * | 2017-08-23 | 2017-10-24 | 合肥市明航养殖有限公司 | A kind of feed addictive for improving egg duck resistance against diseases |
| CN112121067A (en) * | 2020-11-16 | 2020-12-25 | 深圳科兴药业有限公司 | Bifidobacterium infantis microcapsule and preparation method thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1099271A (en) * | 1993-08-23 | 1995-03-01 | 王世荣 | Ecological oral liquid |
-
1995
- 1995-03-06 CN CN95102072A patent/CN1056877C/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1063051C (en) * | 1998-03-27 | 2001-03-14 | 内蒙古自治区畜牧科学院 | Composite live bifidobacterium prepn |
| US6841181B2 (en) | 1999-12-22 | 2005-01-11 | Nutrinova Nutrition Specialties & Food Ingredients Gmbh | Encapsulated multifunctional biologically active food component, process for its production and its use |
| CN107279586A (en) * | 2017-08-23 | 2017-10-24 | 合肥市明航养殖有限公司 | A kind of feed addictive for improving egg duck resistance against diseases |
| CN112121067A (en) * | 2020-11-16 | 2020-12-25 | 深圳科兴药业有限公司 | Bifidobacterium infantis microcapsule and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1056877C (en) | 2000-09-27 |
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