CN111328928A - Antibiotic substitute feed additive - Google Patents

Antibiotic substitute feed additive Download PDF

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Publication number
CN111328928A
CN111328928A CN202010214188.5A CN202010214188A CN111328928A CN 111328928 A CN111328928 A CN 111328928A CN 202010214188 A CN202010214188 A CN 202010214188A CN 111328928 A CN111328928 A CN 111328928A
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group
additive
feed
pigs
cinnamaldehyde
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Inventor
于旭华
林海春
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Guangdong Huihai Agricultural And Animal Husbandry Science And Technology Group Co ltd
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Guangdong Huihai Agricultural And Animal Husbandry Science And Technology Group Co ltd
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/105—Aliphatic or alicyclic compounds
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/158—Fatty acids; Fats; Products containing oils or fats
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/189—Enzymes

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Animal Husbandry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Birds (AREA)
  • Fodder In General (AREA)
  • Feed For Specific Animals (AREA)

Abstract

The invention discloses an antibiotic substitute feed additive. The additive comprises the following components in percentage by weight: 2-8% of bacterial xylanase, 3-5% of acid protease, 1-2% of phytase, 0.2-0.5% of thymol, 0.2-0.5% of cinnamaldehyde, 40-50% of citric acid and 35-45% of corn flour. The additive can obviously improve the intestinal tract morphological development of pigs, effectively improve the growth performance, reduce the feed conversion ratio and realize complete substitution of antibiotics by reasonably compounding the enzyme preparation and the plant essential oil under the condition of completely not adding antibiotics, and has important application prospect in the pig breeding industry.

Description

Antibiotic substitute feed additive
Technical Field
The invention belongs to the technical field of feed additives. More particularly, it relates to an antibiotic substitute feed additive.
Background
Antibiotics have been widely used in the livestock industry as traditional feed additives for the prevention or treatment of livestock diseases. The use of antibiotics plays a great promoting role in large-scale breeding, and the growth of animals is promoted mainly by inhibiting or killing pathogenic microorganisms, reducing the occurrence of digestive tract diseases of the animals and improving the digestibility of animal nutrition. However, the side effects of the disordered use and abuse of antibiotics for a long time are more and more obvious, and the problems of drug residues, drug-resistant bacteria and the like caused by the abuse of the antibiotics seriously affect the healthy development of animal husbandry and even threaten the food safety and human health. With the transformation of animal husbandry, the breeding industry faces strict environmental requirements, and the limitation or disablement of antibiotics in feed has become a necessary trend. Particularly, the use of antibiotics in animal breeding industry has been already absolutely prohibited in countries such as European Union member countries and the United states, so that the implementation of antibiotic-free breeding is an effective means for realizing healthy breeding at present.
Many experts and scholars also develop and research substitutes of antibiotics, and currently, probiotics, prebiotics, organic acids, inorganic antibacterial agents, plant extracts, antibacterial peptides and partial combinations thereof are more, for example, patent CN110506864A discloses a pig feed substitute additive which is composed of organic acids and medium-chain fatty acid monoglyceride and can improve the health of pig intestinal tracts and promote the growth and development of pigs, patent CN103865900B provides a feed complex enzyme containing alkaline xylanase to improve the immunity of raised livestock and poultry, patent CN109463531A discloses a silybum marianum pulp microbial feed which replaces antibiotics and can improve the immunity and resistance of fed objects, but the feed components are more and the preparation method is complex, patent CN108835429A discloses a pig feed additive which is composed of humic acid, phytase, Yuanbao plant extracts, β -glucanase, β -mannase, xylanase, acid protease, tannic acid, sodium butyrate and montmorillonite, has a remarkable protection effect on the intestinal tracts of piglets and can effectively promote the development of the intestinal tracts of the piglets, but the use amount of the pig feed additive can not be completely replaced by antibiotics.
Therefore, the pig feed additive which is simple in component and can completely replace antibiotics is found to have important application value for the development of the pig breeding industry.
Disclosure of Invention
The invention aims to provide a pig feed additive capable of completely replacing antibiotics, which can obviously improve the intestinal health and growth performance of pigs by perfectly matching a specific enzyme preparation and plant essential oil under the condition of completely not adding antibiotics.
The invention aims to provide an antibiotic substitute feed additive.
The invention also aims to provide the application of the additive in preparing the feed for pigs.
Still another object of the present invention is to provide a pig feed.
The above purpose of the invention is realized by the following technical scheme:
the invention provides an antibiotic substitute feed additive, which comprises bacterial xylanase, acid protease, phytase, thymol, cinnamaldehyde, citric acid and corn flour.
The pig feed additive provided by the invention can obviously improve the intestinal health and growth performance of pigs by reasonably compounding the enzyme preparation with thymol and cinnamaldehyde plant essential oil, and compared with an antibiotic group, a plant essential oil group is not added, and other essential oil groups are added, the intestinal health and production performance of pigs are obviously improved. The invention can effectively replace antibiotics, can prevent intestinal diseases of pigs under the condition that the antibiotics are not applicable to the feed, ensures the health and the production performance of the pigs, ensures that the pigs do not contain the antibiotics, and improves the food safety of pork food.
In addition, the feed additive for pigs is added with specific bacterial xylanase, acid protease and phytase, and the citric acid is compounded for promoting the elimination of anti-nutritional factors of arabinoxylan and soybean antigenic protein in the feed, so that the anaphylactic reaction of pig intestinal tracts to soybean protein is reduced, the intestinal health of animals is ensured, the growth performance of the animals is improved, the feed additive can replace antibiotics to be used in the feed for improving the growth performance of the pigs through the reasonable compounding of the enzyme preparation and the citric acid, and the feeding group of the citric acid is more favorable for improving the growth performance of the pigs compared with the lactic acid and acetic acid feeding group.
Preferably, the feed additive comprises the following components in percentage by weight: 2-8% of bacterial xylanase, 3-5% of acid protease, 1-2% of phytase, 0.2-0.5% of thymol, 0.2-0.5% of cinnamaldehyde, 40-50% of citric acid and 35-43.2% of corn flour.
Further preferably, the feed additive comprises the following components in percentage by weight: 5-6% of bacterial xylanase, 4-5% of acid protease, 1-1.5% of phytase, 0.2-0.4% of thymol, 0.3-0.4% of cinnamaldehyde, 45-50% of citric acid and 38.5-42.7% of corn flour.
Most preferably, the feed additive comprises the following components in percentage by weight: 5% of bacterial xylanase, 5% of acid protease, 1% of phytase, 0.2% of thymol, 0.3% of cinnamaldehyde, 50% of citric acid and 38.5% of corn flour.
Preferably, the activity content of the bacterial xylanase is more than or equal to 4 × 105U/kg。
Preferably, the content of the acid protease activity is more than or equal to 2 × 105U/kg。
Preferably, the content of the phytase activity is more than or equal to 1 × 104U/kg。
In addition, the application of the additive in preparing the feed for pigs is also in the protection scope of the invention.
Preferably, the additive is added into the pig feed in an amount of 1-2%.
Finally, a feed for pigs comprising the above additive is also within the scope of the present invention.
Preferably, the swine feed further comprises a basal diet.
The invention has the following beneficial effects:
according to the invention, through reasonable compounding of the enzyme preparation, the plant essential oil and the citric acid, the pig feed additive capable of completely replacing antibiotics is provided, under the condition of completely not using antibiotics, the intestinal health and the growth performance of pigs can be guaranteed, the feed utilization rate is improved, the pigs are ensured not to contain the antibiotics, and the food safety of pork food is improved.
Detailed Description
The present invention is further illustrated by the following specific examples, which are not intended to limit the invention in any way. Reagents, methods and apparatus used in the present invention are conventional in the art unless otherwise indicated. Unless otherwise indicated, reagents and materials used in the following examples are commercially available.
Example 1
AntibioticThe substitute feed additive comprises (by weight percentage) bacterial xylanase (with enzyme activity content of 4 × 10)5U/kg) 5%, acid protease (enzyme activity content 2 × 10)5U/kg) 5%, phytase (enzyme activity content 1 × 10)4U/kg) 1%, thymol 0.2%, cinnamaldehyde 0.3%, citric acid 50% and corn flour 38.5%.
Example 2
An antibiotic as feed additive is prepared from bacterial xylanase (4 × 10 for its enzyme activity)5U/kg) 6%, acid protease (enzyme activity content 2 × 10)5U/kg) 4%, phytase (enzyme activity content 1 × 10)4U/kg) 1.5%, thymol 0.4%, cinnamaldehyde 0.4%, citric acid 45% and corn flour 42.7%;
example 3
An antibiotic as feed additive is prepared from bacterial xylanase (4 × 10 for its enzyme activity)5U/kg) 2%, acid protease (enzyme activity content 2 × 10)5U/kg) 3%, phytase (enzyme activity content 1 × 10)4U/kg) 2%, thymol 0.5%, cinnamaldehyde 0.5%, citric acid 47% and corn flour 45%.
Example 4
An antibiotic as feed additive is prepared from bacterial xylanase (4 × 10 for its enzyme activity)5U/kg) 7%, acid protease (enzyme activity content 2 × 10)5U/kg) 5%, phytase (enzyme activity content 1 × 10)4U/kg) 2%, thymol 0.5%, cinnamaldehyde 0.5%, citric acid 50% and corn flour 35%.
Example 5
An antibiotic as feed additive is prepared from bacterial xylanase (4 × 10 for its enzyme activity)5U/kg) 8%, acid protease (enzyme activity content 2 × 10)5U/kg) 4%, phytase (enzyme activity content 1 × 10)4U/kg) 2%, thymol 0.4%, cinnamaldehyde 0.4%, citric acid 45%, and corn flour 40.2%.
Example 6
First, test method
1. Test animal
480 healthy 110-day-old ternary hybrid pigs are randomly divided into 8 groups according to the weight consistency principle, each group is provided with 5 repetitions, and each repetition is provided with 12 pigs.
2. Test group feeding schedule is shown in table 1
TABLE 1 test grouping
Test group Feeding scheme
Group A: control group Basal diet
Group B: antibiotic group Basal diet + 2% antibiotics
Group C: additive package Basal diet + 2% additive
Group D: additive package Basal diet + 2% additive
Group E: additive package Basal diet + 2% additive
And F group: additive package Basal diet + 2% additive
Group G: additive package Basal diet + 2% additive
Group H: additive package Basal diet + 2% additive
Note: the basic ration is prepared according to the nutrition requirements of poultry released by the Ministry of agriculture of the people's republic of China in 2004.
The additive component in additive group C is bacterial xylanase (with enzyme activity content of 4 × 10)5U/kg) 5%, acid protease (enzyme activity content 2 × 10)5U/kg) 5%, phytase (enzyme activity content 1 × 10)4U/kg) 1%, thymol 0.2%, cinnamaldehyde 0.3%, citric acid 50% and corn flour 38.5%;
the additive group D comprises the following additive components: removing thymol and cinnamaldehyde and complementing the difference with corn flour compared to group C;
the additive group E comprises the following additive components: replacement of thymol with menthol as compared to group C;
the additive group F comprises the following additive components: in comparison to group C, cinnamaldehyde was replaced with eucalyptol.
The additive group G comprises the following additive components: replacement of thymol and cinnamaldehyde with menthol and citral compared to group C;
the additive group H comprises the following additive components: in comparison to group C, thymol and cinnamaldehyde were replaced with eucalyptol and anethole.
3. Feeding mode
The test pre-test period is 3 days, the test period is 49 days, and the test daily ration is granular materials. During the test period, the test pigs feed freely, drink water freely, the colony house keeps clean and dry, the ventilation is good, and immunization and daily management are carried out according to the pig farm regulations.
4. Index measuring method
(1) Growth Performance test
The finishing pigs were weighed on an empty stomach on test days 1, 49 and feed intake was recorded during the test period. After the test is finished, the average daily gain, the average daily feed intake and the feed consumption and weight gain ratio are calculated respectively in the following manner.
Average Daily Gain (ADG): the weight of the pigs was weighed after the end of the test, fasted for 12h before weighing, and the weight of each duplicate was calculated in duplicate. The number of pigs dead in the middle of the trial was recorded and the dead weight was recorded by weighing. The calculation formula is as follows:
average daily gain (g/head/d) ═ average end weight-average initial weight/days of rearing
Average Daily Feed Intake (ADFI): after the feeding test is started, the daily feeding amount and the daily remaining amount are recorded, the dead pigs are obtained, and the feed consumption of the dead pigs is obtained by calculating the difference value of the feeding amount and the remaining amount. The calculation formula is as follows:
average daily food intake (g/head/d) ═ total feed amount-remainder amount)/(pig number × days of feeding)
The ratio of fuel consumption to weight gain (F/G) is total fuel consumption per total weight gain over the test period.
(2) Intestinal health index testing
When the test is finished, 1 test pig with similar weight is selected from each repetition of each group to be slaughtered, and the pH value of the contents in the gastrointestinal section is directly measured by a pH meter; taking duodenum, jejunum and ileum of intestinal tract about 2cm respectively, processing to obtain slices, selecting typical visual field shooting picture with fluorescence microscope, and observing and analyzing intestinal tract morphology in a Leica Qwin image analysis system; collecting duodenal, jejunum and ileum intestinal chyme samples, and detecting the viscosity of chyme by using an Ostwald viscometer.
Second, experimental results
1. Growth Performance of pigs in different test groups
TABLE 2 growth Performance of pigs from different test groups
Experimental groups Average daily food intake (g) Average daily gain (g) Feed conversion ratio (g/g)
Group A 2417 788 3.07
Group B 2403 829 2.90
Group C 2527 878 2.88
Group D 2397 802 2.99
Group E 2397 807 2.97
Group F 2377 814 2.92
Group G 2365 793 2.98
Group H 2370 792 2.99
As can be seen from Table 2, the average daily gain of pigs can be increased and the feed conversion ratio can be reduced by adding a certain amount of essential oil on the basis of the enzyme preparation. The average daily gain of the group C is higher than that of the group B, and the feed meat tends to be reduced compared with the group B, so that the growth performance of the pigs can be effectively improved by reasonably compounding and using the enzyme preparation, the thymol and the cinnamaldehyde; in addition, it can be seen that the effect of group C was better than that of groups D, E, F, G and H, indicating that the feeding group added with thymol and cinnamaldehyde was more advantageous to improve the growth performance of pigs than the other essence-added groups.
1. Analysis of intestinal health index of pigs in different test groups
TABLE 3 intestinal villus crypt ratio analysis of pigs from different test groups
Figure BDA0002423835560000061
Figure BDA0002423835560000071
As can be seen from Table 3, the group A and the group D have relatively poor effects, and the ratio of the intestinal villus height to the crypt depth tends to decrease after thymol and cinnamaldehyde are used on the basis of the group D, wherein the group C has better effect and the effect is very close to that of the antibiotic group, which shows that the additive combination of the invention can be used for replacing antibiotics to improve the health of the porcine intestinal tract, and the ratio of the intestinal villus height to the crypt depth tends to increase after the group E, F, G and the group H replace thymol and cinnamaldehyde, which shows that the addition of other essential oils has no obvious effect on improving the health of the porcine intestinal tract.
TABLE 4 pH analysis of pig gastrointestinal contents of different test groups
Figure BDA0002423835560000072
As can be seen from Table 4, the pH of the gastrointestinal contents of the experimental pigs in the additive group C of the invention tends to be lower than that of the other groups, and gastric juice in a relatively acidic environment is favorable for improving the activity of pepsin and promoting the utilization rate of feed nutrients.
TABLE 5 analysis of the viscosity of chyme in the pig's intestines for different test groups
Figure BDA0002423835560000073
Figure BDA0002423835560000081
As can be seen from table 5, the viscosity of the intestinal chyme can be improved to some extent by adding the additive of the present invention to the basal diet. From the value, the intestinal chyme viscosity can be effectively improved through reasonable compound use of the enzyme preparation and the plant essential oil, and the effect is more obvious than that of an antibiotic group.
Example 7
First, test method
Specific test group feeding regimens are shown in table 6
TABLE 6 test grouping
Test group Feeding scheme
Group A: control group Basal diet
Group B: antibiotic group Basal diet + 2% antibiotics
Group C: additive package Basal diet + 2% additive
Group D: additive package Basal diet + 2% additive
Group E: additive package Basal diet + 2% additive
And F group: additive package Basal diet + 2% additive
Note: the basic ration is prepared according to the nutrition requirements of poultry released by the Ministry of agriculture of the people's republic of China in 2004.
The additive component in additive group C is bacterial xylanase (with enzyme activity content of 4 × 10)5U/kg) 6%, acid protease (enzyme activity content 2 × 10)5U/kg) 4%, phytase (enzyme activity content 1 × 10)4U/kg) 1.5%, thymol 0.4%, cinnamaldehyde 0.4%, citric acid 45% and corn flour 42.7%;
the additive group D comprises the following additive components: comparing to group C, citric acid was removed and the difference was complemented with corn flour;
the additive group E comprises the following additive components: replacing citric acid with acetic acid compared to group C;
the additive group F comprises the following additive components: citric acid was replaced with lactic acid compared to group C.
The other experimental procedures were the same as in example 6.
Second, experimental results
1. Growth Performance of pigs in different test groups
TABLE 7 growth Performance of pigs from different test groups
Experimental groups Average daily food intake (g) Average daily gain (g) Feed conversion ratio (g/g)
Group A 2417 788 3.07
Group B 2403 829 2.90
Group C 2534 870 2.91
Group D 2297 756 3.04
Group E 2434 827 2.94
Group F 2415 810 2.98
As can be seen from Table 7, the average daily gain of pigs was increased and the feed conversion ratio was decreased by adding a certain amount of edible acid to the enzyme preparation compared to group D. The average daily gain of the group C is higher than that of other groups, the feed conversion ratio is equivalent to that of the antibiotic group, and is lower than that of other experimental groups, so that the feed conversion ratio is higher than that of other experimental groups, the feed conversion ratio is equivalent to that of the antibiotic group, the antibiotic can be replaced by the feed through reasonable compound use of the enzyme preparation and the citric acid, the growth performance of the pigs can be improved, and the feeding group of the citric acid is more favorable for improving the growth performance of the pigs than the lactic acid and.
2. Analysis of intestinal health index of pigs in different test groups
TABLE 8 intestinal villus crypt ratio analysis in pigs of different test groups
Experimental groups Ratio of villi to crypt in duodenum Crypt ratio of jejunum villi Ileal villus crypt ratio
Group A 2.24 2.40 2.31
Group B 1.79 1.85 2.09
Group C 1.80 1.94 2.05
Group D 2.21 2.39 2.34
Group E 1.87 2.04 2.11
Group F 1.91 2.01 2.15
As can be seen from table 8, the group a and group D had relatively poor results, and the ratio of intestinal villus height to crypt depth tended to decrease after the edible acid was used on the basis of group D, with group C having a better effect and a very similar effect to the antibiotic group, indicating that the additive combination of the present invention could be used in place of the antibiotic to improve the health of the porcine intestine, and the ratio of intestinal villus height to crypt depth tended to increase after the group E, F replaced citric acid, indicating that the citric acid addition group had the best effect on maintaining the integrity of the porcine intestine structure.
TABLE 9 pH analysis of pig gastrointestinal contents of different test groups
Figure BDA0002423835560000091
Figure BDA0002423835560000101
As can be seen from Table 9, the pH of the gastrointestinal contents of the experimental pigs in the additive group C of the present invention tended to decrease compared with other groups, and gastric juice was in a more acidic environment, which was favorable for improving the activity of pepsin and promoting the utilization of feed nutrients.
TABLE 10 analysis of the viscosity of chyme in the pig's intestines for different test groups
Experimental groups Viscosity of duodenal chyme Viscosity of jejunal chyme Viscosity of ileal chyme
Group A 1.14 1.20 1.20
Group B 1.08 1.13 1.18
Group C 1.05 1.11 1.12
Group D 1.17 1.24 1.25
Group E 1.10 1.16 1.17
Group F 1.09 1.14 1.13
As can be seen from table 10, the viscosity of the intestinal chyme can be improved to some extent by adding the additive of the present invention to the basal diet. From the value, the viscosity of the intestinal chyme can be effectively improved by reasonably compounding the enzyme preparation and the citric acid, and the effect is better than that of an antibiotic group.
In conclusion, the pig feed additive disclosed by the invention is added with specific bacterial xylanase, acid protease and phytase, and the added citric acid can effectively enhance the activity of the acid protease, so that the additive is favorable for eliminating anti-nutritional factors of arabinoxylan and soybean antigenic protein in the feed and reducing the anaphylactic reaction of pig intestinal tracts to the soybean protein, thereby ensuring the intestinal health of animals, being favorable for the morphological development of the pig intestinal tracts and improving the feed conversion rate.
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.

Claims (10)

1. The antibiotic substitute feed additive is characterized by comprising the following components in percentage by weight: 2-8% of bacterial xylanase, 3-5% of acid protease, 1-2% of phytase, 0.2-0.5% of thymol, 0.2-0.5% of cinnamaldehyde, 40-50% of citric acid and 35-45% of corn flour.
2. The additive according to claim 1, comprising the following components in percentage by weight: 5-6% of bacterial xylanase, 4-5% of acid protease, 1-1.5% of phytase, 0.2-0.4% of thymol, 0.3-0.4% of cinnamaldehyde, 45-50% of citric acid and 38.5-42.7% of corn flour.
3. The additive according to claim 1, comprising the following components in percentage by weight: 5% of bacterial xylanase, 5% of acid protease, 1% of phytase, 0.2% of thymol, 0.3% of cinnamaldehyde, 50% of citric acid and 38.5% of corn flour.
4. Additive according to any one of claims 1 to 3, wherein the bacterial xylanase activity content is not less than 4 × 105U/kg。
5. The additive according to any one of claims 1 to 3, wherein the acid protease activity content is not less than 2 × 105U/kg。
6. The additive according to any one of claims 1 to 3, wherein the phytase activity content is not less than 1 × 104U/kg。
7. Use of the additive of any one of claims 1 to 6 in the preparation of a feed for pigs.
8. The use of claim 7, wherein the additive is added to the feed for pigs in an amount of 1% to 2%.
9. A pig feed comprising the additive according to any one of claims 1 to 6.
10. The swine feed of claim 9 further comprising a basal diet.
CN202010214188.5A 2020-03-24 2020-03-24 Antibiotic substitute feed additive Pending CN111328928A (en)

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CN111631310A (en) * 2020-07-13 2020-09-08 四川大想生物科技有限公司 Anti-substitution additive for animal feed and preparation method thereof
CN112042830A (en) * 2020-09-27 2020-12-08 中国农业大学 Broiler feed additive and application thereof
CN114947000A (en) * 2021-02-25 2022-08-30 正百生物科技(江门)有限公司 Antibacterial growth-promoting composite additive for feed and preparation method and application thereof

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111631310A (en) * 2020-07-13 2020-09-08 四川大想生物科技有限公司 Anti-substitution additive for animal feed and preparation method thereof
CN112042830A (en) * 2020-09-27 2020-12-08 中国农业大学 Broiler feed additive and application thereof
CN114947000A (en) * 2021-02-25 2022-08-30 正百生物科技(江门)有限公司 Antibacterial growth-promoting composite additive for feed and preparation method and application thereof
CN114947000B (en) * 2021-02-25 2024-01-19 广州市正百饲料科技有限公司 Antibacterial growth-promoting composite additive for feed and preparation method and application thereof

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