CN111328703A - Device and method for keeping high activity of in-vitro pollen of gramineous crops - Google Patents
Device and method for keeping high activity of in-vitro pollen of gramineous crops Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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Abstract
The invention relates to a device and a method for keeping high activity of in-vitro pollen of gramineous crops. The device for keeping the high activity of the in-vitro pollen of the gramineous crop can quantitatively mix the crushed dry ice with the air containing the pollen when the pollen is collected, so that the effect of rapid sublimation and cooling is achieved, the temperature of a pollen collecting chamber is controlled to be 7-15 ℃ all the time, the refrigeration is rapid and uniform, the pollen collecting time is prolonged, the high activity of the collected pollen is kept, and meanwhile, the CO is used for purifying the pollen2The method has the advantages of inhibiting the respiration of the pollen, reducing the consumption of nutrient substances for storing the pollen, being beneficial to keeping the activity of the pollen, greatly improving the pollen collection quality and finally improving the pollen setting rate.
Description
Technical Field
The invention belongs to the technical field of pollen collection, and particularly relates to a device and a method for keeping high activity of isolated pollen of gramineous crops.
Background
Two varieties with different genetic properties are hybridized, the first filial generation shows very strong fertility, vitality, stress resistance and adaptability, and has more advantages than parents in the aspects of yield, quality and the like; the pollen plays an important role in sexual propagation of plants, and male parent genetic information transmitted after the pollen germinates is the key for seed formation; the collection and storage of pollen and the pollen activity are the foundation of seed reproduction and production and are also important links in the breeding of male sterile lines and the hybrid seed production; before carrying out crop hybrid seed production, a large amount of pollen of male parent materials needs to be collected so as to facilitate hybridization; in order to meet the centralized requirement of large-area female parent pollination in sterile lines of different crops, male parent pollen needs to be collected in advance; how to collect pollen and keep the activity of the pollen is the key of hybrid seed production work.
At present, pollen collection is mainly completed manually and mechanically, the manual pollen collection method has the disadvantages of high labor intensity, low efficiency and uneven pollen collection, and the pollen activity can be reduced faster by manual operation, so that the seed production yield is reduced; in the current mechanical pollen collection process, due to the fact that the temperature is difficult to reduce, the collection time is long, and other conditions are not properly controlled, pollen activity is usually obviously reduced, and the pollen fertilization and fructification capability is seriously influenced.
Disclosure of Invention
The invention aims to solve the problems and provide a device which has a simple structure and reasonable design and can keep the high activity of the isolated pollen of the gramineous crops.
The invention realizes the purpose through the following technical scheme:
the utility model provides a keep gramineae crop separation pollen high-activity device, includes pollen collection room and locates glass bead locker room, drier locker room and the suction fan of pollen collection room upper end, the lower extreme of pollen collection room is connected with inhales the powder ware, it is connected with dry ice ration sprayer on the powder ware to inhale.
As a further optimization scheme of the invention, the powder suction device comprises a shell, a pollen conveying pipe connected to the upper end of the shell, a second filter screen arranged on the inner wall of the shell, a connecting plate connected to one end of the shell, a rotating rod movably connected to the connecting plate and a powder driving operating rod connected to the lower end of the rotating rod.
As a further optimization scheme of the invention, the dry ice quantitative sprayer comprises a dry ice storage box, an electric push rod connected to one end of the dry ice storage box, a piston connected to the output end of the electric push rod and a dry ice spraying pipe connected to the other end of the dry ice storage box, wherein the dry ice spraying pipe is connected to a pollen conveying pipe, a third electromagnetic valve is arranged on the dry ice spraying pipe, and the upper end of the pollen conveying pipe is detachably connected with a pollen collecting chamber.
As a further optimization scheme of the invention, a plurality of pollen boxes are arranged in the pollen collecting chamber, a conveying pipe is arranged in the pollen collecting chamber, and the glass bead storage chamber and the drying agent storage chamber are both connected with the conveying pipe.
As a further optimization scheme of the invention, a first electromagnetic valve is arranged at the joint of the glass bead storage chamber and the conveying pipe, a second electromagnetic valve is arranged at the joint of the desiccant storage chamber and the conveying pipe, and the conveying pipe is communicated with the pollen boxes.
As a further optimization scheme of the invention, a temperature and humidity sensor is arranged on the inner wall of the upper end of the pollen collecting chamber, a first filter screen is arranged on the inner wall of the pollen collecting chamber close to the suction fan, the first filter screen is a melt-blown non-woven fabric or a microfiber filter screen, and the aperture of a screen opening is smaller than 10 μm.
As a further optimization scheme of the invention, the upper end of the rotating rod is connected with a limiting block, the rotating rod is connected with a holding rod, the second filter screen is a plastic net or a non-woven fabric net, and the aperture of the net opening is 60-300 mu m.
As a further optimization scheme of the invention, dry ice particles are arranged in the dry ice storage box, the particle size of the dry ice particles is smaller than 2mm, glass beads are arranged in the glass bead storage chamber, a drying agent is arranged in the drying agent storage chamber, and the drying agent is silica gel.
As a further optimized proposal of the invention, the distance between the powder-removing operating rod and the inlet at the lower end of the shell is 20-30 cm.
A method for maintaining high activity of pollen in vitro of gramineous crops by using the device comprises the following steps:
s1, efficiently collecting pollen through a device;
s2, controlling the spraying amount and the spraying speed of the dry ice by the dry ice quantitative sprayer according to the field temperature and the air inlet flow of pollen collected by the device, mixing the crushed dry ice stored in the dry ice quantitative sprayer with the air containing the pollen, allowing the crushed dry ice to enter a pollen collecting chamber after being mixed with the air containing the pollen, and allowing the dry ice to sublime after entering the pollen collecting chamber and play a role in cooling and supplementing CO2The effect of (1);
and S3, sensing the temperature and the humidity in the pollen collecting box through a temperature and humidity sensor, ensuring that the temperature in the pollen collecting box is always kept between 7 and 15 ℃, and adjusting the temperature through a dry ice quantitative sprayer when the temperature value in the pollen collecting box is abnormal.
The invention has the beneficial effects that:
1) the invention can quantitatively mix the crushed dry ice and the air containing pollen when collecting the pollen, achieves the effect of rapid sublimation and cooling, ensures that the temperature of the pollen collecting chamber is always controlled at 7-15 ℃, the refrigeration is rapid and uniform, prolongs the pollen collecting time, maintains the high activity of the collected pollen and passes through CO simultaneously2The respiration of the pollen is inhibited, the consumption of nutrient substances for storing the pollen is reduced, the vitality of the pollen is kept, the pollen collection quality is greatly improved, and the pollen setting rate is finally improved;
2) the invention has simple structure, high stability, reasonable design and convenient realization.
Drawings
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a view of the powder sucker in cooperation with a dry ice dosing sprayer of the present invention;
fig. 3 is a schematic structural diagram of the fan of the present invention.
In the figure: 1. a pollen collection chamber; 101. a pollen box; 102. a first filter screen; 103. a delivery pipe; 2. a glass bead storage chamber; 201. a first solenoid valve; 3. a desiccant storage compartment; 301. a second solenoid valve; 4. a suction fan; 5. a temperature and humidity sensor; 6. a dry ice quantitative sprayer; 601. an electric push rod; 602. a piston; 603. a dry ice storage bin; 604. a dry ice spray tube; 605. a third electromagnetic valve; 7. a powder suction device; 701. a housing; 702. a second filter screen; 703. a pollen conveying pipe; 704. a connecting plate; 705. a holding rod; 706. rotating the rod; 707. and a powder driving operating rod.
Detailed Description
The present application will now be described in further detail with reference to the drawings, it should be noted that the following detailed description is given for illustrative purposes only and is not to be construed as limiting the scope of the present application, as those skilled in the art will be able to make numerous insubstantial modifications and adaptations to the present application based on the above disclosure.
Example 1
As shown in fig. 1-3, a device for maintaining high activity of in vitro pollen of gramineous crops comprises a pollen collecting chamber 1, a glass bead storage chamber 2, a desiccant storage chamber 3 and a suction fan 4, wherein the glass bead storage chamber 2, the desiccant storage chamber 3 and the suction fan 4 are arranged at the upper end of the pollen collecting chamber 1, the lower end of the pollen collecting chamber 1 is connected with a powder suction device 7, and the powder suction device 7 is connected with a dry ice quantitative sprayer 6;
the powder sucker 7 comprises a shell 701, a pollen conveying pipe 703 connected to the upper end of the shell 701, a second filter screen 702 arranged on the inner wall of the shell 701, a connecting plate 704 connected to one end of the shell 701, a rotating rod 706 movably connected to the connecting plate 704 and a powder driving operating rod 707 connected to the lower end of the rotating rod 706;
the dry ice quantitative sprayer 6 comprises a dry ice storage box 603, an electric push rod 601 connected with one end of the dry ice storage box 603, a piston 602 connected with the output end of the electric push rod 601, and a dry ice spraying pipe 604 connected with the other end of the dry ice storage box 603, wherein the dry ice spraying pipe 604 is connected with a pollen conveying pipe 703, a third electromagnetic valve 605 is arranged on the dry ice spraying pipe 604, the upper end of the pollen conveying pipe 703 and the pollen collecting chamber 1The connection between the two parts is detachable; the dry ice storage box 603 is internally provided with dry ice particles, the particle size of the dry ice particles is smaller than 2mm, the dry ice quantitative sprayer 6 can push the piston 602 to convey dry ice through the electric push rod 601 according to the field temperature and the air inlet flow of collected pollen, the dry ice spraying speed can be regulated and controlled through the pushing speed and the third electromagnetic valve 605 when the dry ice is conveyed, and the lower the field temperature is, the slower the dry ice spraying speed is; the higher the field temperature is, the higher the dry ice spraying speed is, so that the collection chamber can quantitatively mix gaseous CO2The temperature is kept between 7 and 15 ℃ all the time, the temperature and the water content of the pollen in vitro are reduced by rapid sublimation and a large amount of heat absorption and temperature reduction, and the high activity of the pollen is kept; at the same time due to the mixed CO2The respiration of the pollen can be inhibited, the consumption of nutrient substances for storing the pollen is reduced, and the pollen vitality is kept;
a plurality of pollen boxes 101 are arranged in the pollen collecting chamber 1, the pollen boxes 101 are used for storing collected pollen, a conveying pipe 103 is arranged in the pollen collecting chamber 1, the glass bead storage chamber 2 and the desiccant storage chamber 3 are both connected with the conveying pipe 103, and the conveying pipe 103 can convey objects stored in the glass bead storage chamber 2 and the desiccant storage chamber 3 to the pollen boxes 101;
a first electromagnetic valve 201 is arranged at the joint of the glass bead storage chamber 2 and the conveying pipe 103, a second electromagnetic valve 301 is arranged at the joint of the desiccant storage chamber 3 and the conveying pipe 103, the conveying pipe 103 is communicated with the plurality of pollen boxes 101, glass beads are arranged in the glass bead storage chamber 2, a desiccant is arranged in the desiccant storage chamber, the desiccant is silica gel, the first electromagnetic valve 201 can be regulated and controlled to quantitatively spray the glass beads to the pollen boxes 101 according to the collected pollen amount so as to prevent the collected pollen from agglomerating and keep the high activity of the pollen, meanwhile, the second electromagnetic valve 301 can be regulated and controlled to quantitatively spray the desiccant to the pollen boxes 101 according to the collected pollen amount so as to keep the humidity in the pollen collection chamber 1 at 10% -20% all the time and keep the high activity of the pollen;
a temperature and humidity sensor 5 is arranged on the inner wall of the upper end of the pollen collecting chamber 1, so that the temperature and the humidity in the pollen collecting chamber 1 can be monitored at any time;
a first filter screen 102 is arranged on the inner wall of the pollen collecting chamber 1 and close to the suction fan 4, the first filter screen 102 is a melt-blown non-woven fabric or microfiber filter screen, the aperture of the mesh opening is less than 10 μm, and the first filter screen is preferably in a honeycomb or folding shape, so that the dissipation of pollen can be prevented;
the second filter screen 702 is a plastic net or a non-woven net, and the aperture of the net opening is 60-300 μm so as to remove impurities such as anther, insects, broken leaves and the like;
the upper end of the rotating rod 706 is connected with a limiting block, and the rotating rod 706 is connected with a holding rod 705; the distance between the powder driving operating rod 707 and the inlet at the lower end of the shell 701 is 20-30cm, the powder driving operating rod 707 can be driven by the holding rod 705 to shake off pollen from the ear heads and disperse the pollen above the ear heads in a centralized manner, so that the powder absorber 7 can absorb a large amount of powder in a centralized manner, and the efficiency of collecting the pollen is obviously improved;
in the process of pollen collection through the device, the suction fan 4 is firstly controlled to start working and generate airflow, the airflow sucks powder from the powder sucking device 7, meanwhile, the pollen is shaken off from the ear head by the powder driving operating rod 707 on the powder sucking device 7 and is dispersed above the ear head in a centralized manner, so that the powder sucking device 7 can suck a large amount of concentrated powder, and when the pollen is conveyed into the pollen collection chamber 1, the dry ice is conveyed into the pollen conveying pipe 703 through the dry ice quantitative sprayer 6, so that the pollen and dry ice mixture enters the pollen collection chamber 1.
The method for maintaining the high activity of the isolated pollen of the gramineous crops by applying the device comprises the following steps:
s1, efficiently collecting pollen by the device;
s2, controlling the dry ice spraying amount and the spraying speed by the dry ice quantitative sprayer 6 according to the field temperature and the air inlet flow of pollen collected by the device, so that the crushed dry ice stored in the dry ice quantitative sprayer 6 is mixed with the air containing the pollen, the crushed dry ice is mixed with the air containing the pollen and then enters the pollen collecting chamber 1, and the dry ice sublimes after entering the pollen collecting chamber 1 and has the effects of cooling and supplementing CO 2;
and S3, sensing the temperature and the humidity in the pollen collecting box 1 through a temperature and humidity sensor 5 to ensure that the temperature in the pollen collecting box 1 is always kept at 7-15 ℃, and adjusting the temperature through a dry ice quantitative sprayer 6 when the temperature value in the pollen collecting box 1 is abnormal, wherein the refrigeration effect of the dry ice quantitative sprayer 6 is rapid and uniform, the pollen collecting time is prolonged, the high activity of the collected pollen is maintained, the respiration of the pollen is inhibited through CO2, the consumption of nutrient substances stored in the pollen is reduced, the pollen activity is favorably maintained, the pollen collecting quality is greatly improved, and the pollen maturing rate is finally improved.
In order to verify that the activity of the pollen collected by the device and the method in the embodiment is compared with the activity of fresh pollen in vitro, the following experiment is carried out and the comparison is carried out according to actual data, wherein the pollen collected by the device and the method in the embodiment takes rice seeds as an example; the male parent is YR0822, and the female parent is 1892S;
planting of materials
Planting male parents and female parents in a combined fertilizer test base of Anhui province in 2019 for 5 months; wherein, the female parent 1892S is planted in 3 field blocks (the specifications of the field block A, the field block B and the field block C are all consistent, the area is 2000 square meters) in a centralized way, so that the initial ear-bearing flowering period of the female parent 1892S is centralized in about 8 months and 17 days; wherein, the field A and the field B are planted in the same field as the male parent and the female parent (1 row of the male parent: 12 rows of the female parent); the field block A is a reference field block, and the artificial flour driving is utilized to carry out seed production according to a conventional hybrid seed production method; before the ear is started, the field B is in the same growth condition as the field A to keep the sterile line, the male parent is cut off in advance, and the pollen collected by the method is adopted for pollination; planting female parents in the whole field of the field C, and pollinating by adopting the pollen collected by the method; in addition, the male parent YR0822 is planted in the field in large scale twenty days ahead of the female parent 1892S.
Secondly, collecting pollen
Pollen is collected by the device and the method in the full-bloom period of the male parent YR0822, and fresh pollen is collected by a conventional method for comparison.
Third, pollen vitality determination and verification
The pollen collected in the second step was taken out of the pollen collection room 1 and three experiments were performed to verify the pollen viability. Firstly, pollen is taken out and directly used for measuring pollen viability; secondly, the collected pollen is awarded to a sterile line 1892S with glume cutting in advance and then sleeved with a kraft paper bag, and the pollen setting rate is counted after 30 days; third, the collected pollen was applied to field a, field B and field C of female parent 1892S field of example two, and the yield was measured after collection.
1. Determination of pollen viability
Pollen viability was measured by tetrazolium staining, and the pollen collected in example 1 was used as the experimental group and fresh pollen was used as the control group.
①, picking a small amount of pollen from the forceps tips, placing on a glass slide, and repeating for 10 times;
②, dropping 1-2 drops of 0.5% tetrazole solution (0.5g tetrazole dissolved in 100ml of PH7.0 phosphate buffer), and covering with a cover glass;
③, dyeing at 35 ℃, and observing by a microscope;
the red dyed pollen is viable pollen, and the colorless or light red pollen is non-viable or weak-viable pollen.
TABLE 1 determination of pollen viability collected in example 1 versus control pollen viability
Pollen viability assay results (table 1): the pollen viability collected in example 1 averaged 89.9%, the control, i.e., fresh pollen viability, averaged 91.7%, and there was no significant difference in pollen viability.
2. Determination of pollen setting percentage
The pollen collected by the invention is awarded to a sterile line 1892S with glume cutting in advance, and then is sleeved with a kraft paper bag for 10 times of repetition, the pollen setting rate is counted after 30 days, and meanwhile, fresh pollen is used as a reference. The results show (table 2): the pollination seed setting rate of the pollen collected in the example 1 is 63.9 percent, the control namely the fresh pollen seed setting rate is 68.0 percent, and the seed setting rate has no obvious difference.
TABLE 2 pollen Collection vs. control pollen set percentage results in example 1
3. Determination of field yield
And (3) harvesting hybrid seeds respectively corresponding to the field rice in the mature period, wherein the yield of the field B is increased by about 5.5 percent compared with the field A, the yield of the field C is increased by about 9.3 percent compared with the field A, and the yield of the field C is increased by about 6.8 percent compared with the field B after harvesting, airing, yield measurement and the like.
The data show that the device and the method can keep the high activity of the collected pollen for a longer time, greatly improve the pollen collection efficiency, finally improve the pollen setting rate and increase the seed production yield.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention.
Claims (10)
1. The utility model provides a keep gramineae crop separation pollen high-activity device, includes pollen collection chamber (1) and locates glass bead locker room (2), drier locker room (3) and suction fan (4) of pollen collection chamber (1) upper end, the lower extreme of pollen collection chamber (1) is connected with inhales whitewashed ware (7), its characterized in that: the powder suction device (7) is connected with a dry ice quantitative spraying device (6).
2. The device for maintaining high viability of isolated pollen of gramineous crops as claimed in claim 1, wherein: the powder suction device (7) comprises a shell (701), a pollen conveying pipe (703) connected to the upper end of the shell (701), a second filter screen (702) arranged on the inner wall of the shell (701), a connecting plate (704) connected to one end of the shell (701), a rotating rod (706) movably connected to the connecting plate (704) and a powder driving operating rod (707) connected to the lower end of the rotating rod (706).
3. The device for maintaining high activity of pollen isolated from gramineous crops as claimed in claim 2, wherein: the dry ice quantitative sprayer (6) comprises a dry ice storage box (603), an electric push rod (601) connected to one end of the dry ice storage box (603), a piston (602) connected to the output end of the electric push rod (601), and a dry ice spraying pipe (604) connected to the other end of the dry ice storage box (603), wherein the dry ice spraying pipe (604) is connected to a pollen conveying pipe (703), a third electromagnetic valve (605) is arranged on the dry ice spraying pipe (604), and the upper end of the pollen conveying pipe (703) is detachably connected with a pollen collecting chamber (1).
4. The device for maintaining high viability of isolated pollen of gramineous crops as claimed in claim 1, wherein: the pollen collecting chamber is characterized in that a plurality of pollen boxes (101) are arranged in the pollen collecting chamber (1), conveying pipes (103) are arranged in the pollen collecting chamber (1), and the glass bead storage chamber (2) and the drying agent storage chamber (3) are connected with the conveying pipes (103).
5. The device for maintaining high viability of isolated pollen of gramineous crops as claimed in claim 4, wherein: the junction of glass bead locker room (2) and conveyer pipe (103) is equipped with first solenoid valve (201), the junction of drier locker room (3) and conveyer pipe (103) is equipped with second solenoid valve (301), conveyer pipe (103) all communicate with a plurality of pollen box (101).
6. The device for maintaining high viability of isolated pollen of gramineous crops as claimed in claim 1, wherein: the pollen collecting device is characterized in that a temperature and humidity sensor (5) is arranged on the inner wall of the upper end of the pollen collecting chamber (1), a first filter screen (102) is arranged at a position, close to the suction fan (4), on the inner wall of the pollen collecting chamber (1), the first filter screen (102) is a melt-blown non-woven fabric or a microfiber filter screen, and the aperture of a screen opening is smaller than 10 microns.
7. The device for maintaining high activity of pollen isolated from gramineous crops as claimed in claim 2, wherein: the upper end of the rotating rod (706) is connected with a limiting block, the rotating rod (706) is connected with a holding rod (705), the second filter screen (702) is a plastic net or a non-woven fabric net, and the aperture of the net opening is 60-300 mu m.
8. The device for maintaining high viability of isolated pollen of gramineous crops as claimed in claim 3, wherein: the dry ice storage box (603) is internally provided with dry ice particles, the particle size of the dry ice particles is smaller than 2mm, glass beads are arranged in the glass bead storage chamber (2), and a drying agent is arranged in the drying agent storage chamber and is silica gel.
9. The device for maintaining high viability of isolated pollen of gramineous crops as claimed in claim 1, wherein: the distance between the powder removing operating rod (707) and the inlet at the lower end of the shell (701) is 20-30 cm.
10. A method for maintaining high viability of isolated pollen of a gramineous crop by applying a device according to any one of claims 1 to 9, comprising the steps of:
s1, efficiently collecting pollen through a device;
s2, controlling the dry ice spraying amount and the spraying speed by the dry ice quantitative sprayer (6) according to the field temperature and the air inlet flow of pollen collected by the device, mixing the crushed dry ice stored in the dry ice quantitative sprayer (6) with the air containing pollen, enabling the crushed dry ice to enter the pollen collecting chamber (1) after being mixed with the air containing pollen, sublimating the dry ice after entering the pollen collecting chamber (1), and cooling and supplementing CO2The effect of (1);
and S3, sensing the temperature and the humidity in the pollen collecting box (1) through a temperature and humidity sensor (5), ensuring that the temperature in the pollen collecting box (1) is always kept between 7 and 15 ℃, and adjusting through a dry ice quantitative sprayer (6) when the temperature value in the pollen collecting box (1) is abnormal.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010293260.8A CN111328703B (en) | 2020-04-15 | 2020-04-15 | Device and method for maintaining high activity of in-vitro pollen of gramineous crops |
| PCT/CN2021/078675 WO2021208618A1 (en) | 2020-04-15 | 2021-03-02 | Apparatus and method for keeping high vitality of in-vitro pollen of gramineous crops |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010293260.8A CN111328703B (en) | 2020-04-15 | 2020-04-15 | Device and method for maintaining high activity of in-vitro pollen of gramineous crops |
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| CN111328703A true CN111328703A (en) | 2020-06-26 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021208618A1 (en) * | 2020-04-15 | 2021-10-21 | 安徽荃银高科种业股份有限公司 | Apparatus and method for keeping high vitality of in-vitro pollen of gramineous crops |
| CN113875576A (en) * | 2021-09-28 | 2022-01-04 | 安徽荃银高科种业股份有限公司 | Air-suction type high-activity pollen collecting and cooling device and method thereof |
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- 2020-04-15 CN CN202010293260.8A patent/CN111328703B/en active Active
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| CN201378990Y (en) * | 2009-03-24 | 2010-01-13 | 中棉种业科技股份有限公司 | Electric pollen collector for cotton |
| JP2017143770A (en) * | 2016-02-16 | 2017-08-24 | 香川県 | Pollen collector |
| CN108684521A (en) * | 2018-08-02 | 2018-10-23 | 湖北谷神科技有限责任公司 | Hybrid seed production methods and pollen apparatus for temporary storage |
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| CN109874663A (en) * | 2019-04-17 | 2019-06-14 | 安徽荃银高科种业股份有限公司 | A kind of method of gramineous crop cenospecies large-scale production |
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| WO2021208618A1 (en) * | 2020-04-15 | 2021-10-21 | 安徽荃银高科种业股份有限公司 | Apparatus and method for keeping high vitality of in-vitro pollen of gramineous crops |
| CN113875576A (en) * | 2021-09-28 | 2022-01-04 | 安徽荃银高科种业股份有限公司 | Air-suction type high-activity pollen collecting and cooling device and method thereof |
Also Published As
| Publication number | Publication date |
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| WO2021208618A1 (en) | 2021-10-21 |
| CN111328703B (en) | 2024-02-02 |
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