CN111304074A - Multifunctional microorganism culture tank and application thereof - Google Patents
Multifunctional microorganism culture tank and application thereof Download PDFInfo
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- CN111304074A CN111304074A CN202010244129.2A CN202010244129A CN111304074A CN 111304074 A CN111304074 A CN 111304074A CN 202010244129 A CN202010244129 A CN 202010244129A CN 111304074 A CN111304074 A CN 111304074A
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- culture tank
- microorganism culture
- sealing ring
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- cover
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/54—Constructional details, e.g. recesses, hinges hand portable
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/04—Seals
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
Abstract
The invention discloses a multifunctional anaerobic culture tank and application thereof, wherein the culture tank comprises: the device comprises a double-cylinder microorganism culture tank, a culture tank cover arranged on the double-cylinder microorganism culture tank, a sealing ring fixed in the culture tank cover, and an outer layer sealing assembly arranged between the double-cylinder microorganism culture tank and the culture tank cover; the sealing ring and the top edge of the double-cylinder microorganism culture tank form a complementary structure; according to the invention, the sealing ring and the top edge of the double-barrel microorganism culture tank form a complementary structure, so that the sealing property is increased, and the gas environment with specific trace oxygen concentration can be accurately controlled by matching with the outer layer sealing assembly.
Description
Technical Field
The invention relates to a detection auxiliary device, in particular to a multifunctional anaerobic culture tank and application thereof.
Background
Campylobacter belongs to the main cause of human food-borne diarrhea diseases, and is the most common bacterium which is pointed out by WHO statistics of world health organization to cause gastroenteritis all over the world. The high incidence of campylobacter diarrhoea, coupled with its course and possible fatal sequelae, makes this disease of great importance from a socioeconomic point of view. According to the national food safety standard of China, the culture and detection of campylobacter need to provide a microaerophilic environment with 5% oxygen concentration for the campylobacter. The food safety standard also covers a plurality of anaerobic bacteria such as shigella, clostridium botulinum, bifidobacterium, clostridium perfringens and the like, and the optimal gas environment for anaerobic bacteria culture is an anaerobic environment with 0% oxygen concentration, so a good microaerophilic and anaerobic bacteria culture tool is needed in a vast food safety detection mechanism. Compared with the conventional aerobic culture tool such as a constant temperature incubator, the tool needs a special sealed and airtight cavity design, and avoids the possibility of gas exchange and air leakage in the culture process; so that the tools can ensure that the tools can provide a constant microaerophilic or anaerobic environment for the culture of campylobacter and anaerobe with the help of the gas pumping replacement tool or the anaerobic catalyst. The anaerobic culture box/culture tank provided in the market at present has the problems of poor tightness, low sealing operation efficiency, low culture flux and the like, so that the overall culture detectable rate of campylobacter and anaerobic bacteria is reduced, and the culture effect is unstable.
The working mode of the anaerobic culture box/anaerobic culture tank is as follows: placing a microorganism culture carrier such as a culture dish containing anaerobic bacteria or microaerophilic bacteria into an opened anaerobic box/anaerobic tank, then placing an anaerobic catalyst, and closing the tank cover; an O-shaped rubber sealing ring is arranged between the tank body and the tank cover, the sealing clamp connected with the tank cover of the tank body is clamped when the tank cover is closed, and the anaerobic or microaerophilic environment is realized in the closed tank body. The problems of the current design are as follows:
the sealing property is not good enough: at present, the anaerobic tank on the market is closed very depending on that an O-shaped rubber sealing ring is in a good state, if the rubber of the sealing ring is aged, cracks can appear, the hidden danger of air leakage occurs, and the sealing property is poor.
The sealing operation efficiency is low: at present, in a sealing mode, after cultures need to be placed into the tank body and the tank cover at each time, the sealing clamps connected with the tank body and the tank cover are manually clamped one by one, usually, 4 sealing clamps are arranged, 4 operations are needed, and the operation efficiency is reduced.
Poor control of microaerophilic gas environment: microaerophilic bacteria such as campylobacter and the like need to be controlled in a gas environment with a specific trace oxygen concentration, and air with 20% oxygen concentration is mixed into the anaerobic tank due to poor sealing performance of the original tank body, so that more oxygen enters the anaerobic tank, and a specific accurate oxygen concentration is not formed in the final tank body.
The market needs an anaerobic culture tank which can accurately control the gas environment with specific trace oxygen concentration and has good tightness, and the invention solves the problems.
Disclosure of Invention
In order to solve the defects of the prior art, the invention aims to provide a multifunctional anaerobic culture tank and application thereof.
In order to achieve the above object, the present invention adopts the following technical solutions:
a multi-functional microbial cultivation tank, comprising: the device comprises a double-cylinder microorganism culture tank, a culture tank cover arranged on the double-cylinder microorganism culture tank, a sealing ring fixed in the culture tank cover, and an outer layer sealing assembly arranged between the double-cylinder microorganism culture tank and the culture tank cover; the sealing ring and the top edge of the double-barrel microorganism culture tank form a complementary structure.
The multifunctional microorganism culture tank comprises a sealing ring: an upper sealing ring and a lower sealing ring; the top edge of the double-cylinder microorganism culture tank comprises: the complementary upper opening of the upper sealing ring and the complementary lower opening of the lower sealing ring.
In the multifunctional microorganism culture tank, the volume of the upper sealing ring is larger than that of the lower sealing ring.
In the multifunctional microorganism culture tank, the section of the sealing ring is trapezoidal, and the section of the top edge of the double-cylinder microorganism culture tank is trapezoidal.
In the multifunctional microorganism culture tank, the sealing ring is arranged in the slit on the inner side of the culture tank cover.
In the multifunctional microorganism culture tank, the outer sealing component comprises: be fixed in the cover layering of cultivateing the cover both sides, will irrigate the fastening pressure that the layering was fixed on cultivateing the cover and collude the subassembly, set up in easy plug interface and the sealing valve on the top of cultivateing the cover.
The fastening pressure hook component of the multifunctional microorganism culture tank is provided with: the fastening pressure hook body is clamped and fixed on the irrigation layering, and the pressure spring is connected in the fastening pressure hook.
According to the multifunctional microorganism culture tank, the tank cover handle is arranged on the culture tank cover.
The application of the multifunctional microorganism culture tank is used for culturing the campylobacter and the anaerobic bacteria.
The invention has the advantages that:
according to the invention, the sealing ring and the top edges of the double-barrel microorganism culture tank form a complementary structure, and the lower edge of the sealing ring and the upper edge of the tank body are both of symmetrical trapezoidal structures, so that the sealing property is improved, the strict sealing between the tank body and the tank cover is ensured, and the possibility of air leakage is avoided;
the complementary structure is matched with an easy-to-plug interface and a sealing valve, so that the gas environment with specific trace oxygen concentration can be accurately controlled, and the culture of micro aerobic bacteria such as campylobacter jejuni and the like is optimized;
the structural design of the outer layer sealing component simplifies the anaerobic sealing operation, thereby improving the efficiency;
due to the sealing design, the anaerobic culture of anaerobic bacteria can be realized when the anaerobic catalyst is built in or the gas pumping and discharging method is adopted for vacuumizing, and the microaerophilic culture of microaerophilic bacteria can be realized when the microaerophilic catalyst is built in, so that the multifunctional application is realized.
Drawings
FIG. 1 is an exploded view of one embodiment of the present invention;
fig. 2 is a schematic structural diagram of an embodiment of the present invention.
The meaning of the reference symbols in the figures:
1 binocular microorganism culture tank, 2 culture tank cover, 3 trapezoidal sealing rings, 4 tank cover layering, 5 tank cover handles, 6 fastening pressure colludes the body, 7 compression spring, 8 easy plug interfaces and sealing valve.
Detailed Description
The invention is described in detail below with reference to the figures and the embodiments.
A multifunctional microorganism culture tank as shown in fig. 1 and 2, comprising: the double-cylinder microorganism culture tank comprises a double-cylinder microorganism culture tank 1, a culture tank cover 2 arranged on the double-cylinder microorganism culture tank 1, a sealing ring fixed in the culture tank cover 2, and an outer layer sealing assembly arranged between the double-cylinder microorganism culture tank 1 and the culture tank cover 2; the sealing ring forms a complementary structure with the top edge of the double-cylinder microorganism culture tank 1, and the sealing ring is installed in the inner side slit of the culture tank cover 2. The anaerobic culture tank body connected with the double tanks is in a double-cylinder shape, and a culture dish can be placed in each cylinder independently.
The sealing washer is formed by: an upper sealing ring and a lower sealing ring; the top edge of the double-cylinder microorganism culture tank 1 comprises: the complementary upper opening of the upper sealing ring and the complementary lower opening of the lower sealing ring. Preferably, the volume of the upper sealing ring is larger than that of the lower sealing ring; as shown in fig. 1, it is still further preferred that the complementary structure is a trapezoidal complementary structure; the section of the sealing ring is trapezoidal, and the section of the top edge of the double-barrel microorganism culture tank 1 is trapezoidal. It should be noted that: the complementary structure is not only a complementary structure of a trapezoid, but also the shape is not limited. However, the invention adopts a trapezoidal sealing design, and the top of the double-cylinder microorganism culture tank 1 adopts a trapezoidal opening with a large upper part and a small lower part to form trapezoidal complementation with the trapezoidal sealing ring 3 with a small upper part and a large lower part; the design of big end up can further improve sealing performance.
The outer seal assembly is composed of: be fixed in the cover layering 4 of cultivateing 2 both sides of cover, will irrigate the layering and fix the fastening pressure on the cultivation cover and collude the subassembly, set up in easy plug interface and the sealing valve 8 on the top of cultivation cover. The fastening pressing hook component comprises: a fastening pressure hook body 6 which is clamped and fixed on the irrigation layering, and a pressure spring 7 which is connected in the fastening pressure hook. For convenient transportation, a tank cover handle 5 is arranged on the culture tank cover 2.
The specific working mode of the culture cavity sealing is as follows: firstly, respectively putting cultures such as culture dishes into the double-tank bodies; then a trapezoidal sealing ring 3 is arranged in a slit on the inner side of the tank cover; the cross section of the sealing ring is trapezoidal, so that the sealing ring is staggered and complementary with the top of the tank body; and finally, after the culture tank cover 2 with 2 fastening pressure hook assemblies is closed on the tank body, the 2 fastening pressure hook assemblies are put down and compressed, the sealing ring and the top edge of the complementary double-cylinder microorganism culture tank 1 form mutual limiting, and the whole anaerobic tank is sealed.
The application of the microorganism culture tank is used for culturing the campylobacter and the anaerobic bacteria; the novel microorganism culture tank can ensure sealing, so the novel microorganism culture tank can be used for anaerobic culture of anaerobic bacteria (such as shigella, bifidobacterium and the like) when an anaerobic catalyst is arranged in the novel microorganism culture tank (the catalyst and oxygen in the air in the cavity are subjected to catalytic reaction to consume all the oxygen in the sealed cavity) or a gas pumping and discharging method is adopted for vacuumizing (the vacuumizing is connected to an easy-plugging interface to realize), and can be used for microaerophilic culture of microaerophilic bacteria (such as campylobacter jejuni, brucella and the like) when the oxygen concentration proportion is reduced to 5-10% from about 20% of normal air by the catalyst in the novel microorganism culture tank.
The sealing ring and the top edge of the double-barrel microorganism culture tank 1 form a complementary structure, so that the strict sealing of the tank body and the tank cover is ensured, and the possibility of air leakage is avoided; because the tightness is good, the application range of the culture tank is expanded, and multiple functions are realized; the traditional anaerobic culture tank sealing mode needs to operate the clamping operation of the sealing clamp for many times, the novel tank cover only needs 2 sealing pressure hooks, and the tank body and the tank cover can be tightly pressed by simply pressing the sealing pressure hooks downwards to realize the closing of the tank body; when the anaerobic culture tank body is closed, the tank body and the tank cover can be closed and sealed by adopting a trapezoidal sealing method; thereby greatly simplifying the sealing operation and greatly improving the efficiency of the sealing operation. A double-tank design is adopted, namely 2 tank bodies share the same tank cover; this design has realized once switching cover operation, can realize that the effect of anaerobic culture dish is put into simultaneously to two jars, improves the flux. Because the sealing performance of the tank body is greatly improved, air containing 20% of oxygen concentration cannot be mixed into the anaerobic tank, the specific certain accurate oxygen concentration can be formed in the final tank body, the optimal oxygen concentration is provided for microaerophilic bacteria such as campylobacter and the like, and the culture effect of microaerophilic bacteria such as campylobacter and the like is improved.
The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It should be understood by those skilled in the art that the above embodiments do not limit the present invention in any way, and all technical solutions obtained by using equivalent alternatives or equivalent variations fall within the scope of the present invention.
Claims (9)
1. A multi-functional microbial cultivation jar, comprising: the double-cylinder microorganism culture tank comprises a double-cylinder microorganism culture tank, a culture tank cover arranged on the double-cylinder microorganism culture tank, a sealing ring fixed in the culture tank cover, and an outer layer sealing assembly arranged between the double-cylinder microorganism culture tank and the culture tank cover; the sealing ring and the top edge of the double-barrel microorganism culture tank form a complementary structure.
2. The multifunctional microorganism culture tank as claimed in claim 1, wherein the sealing ring comprises: an upper sealing ring and a lower sealing ring; the top edge of the double-cylinder microorganism culture tank comprises: the complementary upper opening of the upper sealing ring and the complementary lower opening of the lower sealing ring.
3. The multifunctional microorganism culture tank of claim 2, wherein the upper sealing ring has a larger volume than the lower sealing ring.
4. The multi-functional microorganism culture tank as claimed in claim 3, wherein the cross-section of the packing is trapezoidal, and the cross-section of the top edge of the double-tube microorganism culture tank is trapezoidal.
5. The multifunctional microorganism culture tank as claimed in claim 1, wherein the packing is fitted in the inner slit of the lid of the culture tank.
6. The multifunctional microorganism culture tank as claimed in claim 1, wherein the outer sealing member comprises: be fixed in the cover layering of cultivateing the cover both sides, will irrigate the fastening pressure that the layering was fixed on cultivateing the cover and collude the subassembly, set up in easy plug interface and the sealing valve on the top of cultivateing the cover.
7. The multifunctional microorganism culture tank as claimed in claim 6, wherein the fastening and pressing hook component comprises: the fastening pressure hook body is clamped and fixed on the irrigation layering, and the pressure spring is connected in the fastening pressure hook.
8. The multifunctional microorganism culture tank as claimed in claim 1, wherein the culture tank cover is provided with a tank cover handle.
9. The use of a multifunctional microbial cultivation tank as claimed in claim 1, which is used for cultivating Campylobacter and anaerobe.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010244129.2A CN111304074A (en) | 2020-03-31 | 2020-03-31 | Multifunctional microorganism culture tank and application thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010244129.2A CN111304074A (en) | 2020-03-31 | 2020-03-31 | Multifunctional microorganism culture tank and application thereof |
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| CN111304074A true CN111304074A (en) | 2020-06-19 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115746998A (en) * | 2022-11-08 | 2023-03-07 | 杭州华端生物科技有限公司 | Anaerobic culture tank |
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2020
- 2020-03-31 CN CN202010244129.2A patent/CN111304074A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115746998A (en) * | 2022-11-08 | 2023-03-07 | 杭州华端生物科技有限公司 | Anaerobic culture tank |
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