CN111296641B - Biological organic mineral composite additive for reducing dark spot of eggs and application method thereof - Google Patents
Biological organic mineral composite additive for reducing dark spot of eggs and application method thereof Download PDFInfo
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- CN111296641B CN111296641B CN202010322467.3A CN202010322467A CN111296641B CN 111296641 B CN111296641 B CN 111296641B CN 202010322467 A CN202010322467 A CN 202010322467A CN 111296641 B CN111296641 B CN 111296641B
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- 239000002131 composite material Substances 0.000 title claims abstract description 69
- 235000013601 eggs Nutrition 0.000 title claims abstract description 66
- 239000000654 additive Substances 0.000 title claims abstract description 63
- 230000000996 additive effect Effects 0.000 title claims abstract description 56
- 229910052500 inorganic mineral Inorganic materials 0.000 title claims abstract description 50
- 239000011707 mineral Substances 0.000 title claims abstract description 45
- 230000001603 reducing effect Effects 0.000 title claims abstract description 14
- 238000000034 method Methods 0.000 title abstract description 12
- 235000010755 mineral Nutrition 0.000 claims abstract description 44
- 229940088594 vitamin Drugs 0.000 claims abstract description 33
- 229930003231 vitamin Natural products 0.000 claims abstract description 33
- 235000013343 vitamin Nutrition 0.000 claims abstract description 33
- 239000011782 vitamin Substances 0.000 claims abstract description 33
- 239000000419 plant extract Substances 0.000 claims abstract description 31
- 239000011573 trace mineral Substances 0.000 claims abstract description 30
- 235000013619 trace mineral Nutrition 0.000 claims abstract description 30
- 230000017448 oviposition Effects 0.000 claims description 40
- 239000000284 extract Substances 0.000 claims description 25
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 22
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 claims description 22
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims description 22
- 229910052802 copper Inorganic materials 0.000 claims description 22
- 239000010949 copper Substances 0.000 claims description 22
- 239000011572 manganese Substances 0.000 claims description 22
- 229910052748 manganese Inorganic materials 0.000 claims description 22
- 239000011701 zinc Substances 0.000 claims description 22
- 229910052725 zinc Inorganic materials 0.000 claims description 22
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 claims description 20
- 150000001875 compounds Chemical class 0.000 claims description 18
- 229930003316 Vitamin D Natural products 0.000 claims description 11
- QYSXJUFSXHHAJI-XFEUOLMDSA-N Vitamin D3 Natural products C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@H](C)CCCC(C)C)=C/C=C1\C[C@@H](O)CCC1=C QYSXJUFSXHHAJI-XFEUOLMDSA-N 0.000 claims description 11
- 235000019166 vitamin D Nutrition 0.000 claims description 11
- 239000011710 vitamin D Substances 0.000 claims description 11
- 150000003710 vitamin D derivatives Chemical class 0.000 claims description 11
- 229940046008 vitamin d Drugs 0.000 claims description 11
- FPIPGXGPPPQFEQ-UHFFFAOYSA-N 13-cis retinol Natural products OCC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-UHFFFAOYSA-N 0.000 claims description 10
- FPIPGXGPPPQFEQ-BOOMUCAASA-N Vitamin A Natural products OC/C=C(/C)\C=C\C=C(\C)/C=C/C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-BOOMUCAASA-N 0.000 claims description 10
- 229930003451 Vitamin B1 Natural products 0.000 claims description 10
- 229930003779 Vitamin B12 Natural products 0.000 claims description 10
- 229930003427 Vitamin E Natural products 0.000 claims description 10
- FPIPGXGPPPQFEQ-OVSJKPMPSA-N all-trans-retinol Chemical compound OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-OVSJKPMPSA-N 0.000 claims description 10
- FDJOLVPMNUYSCM-WZHZPDAFSA-L cobalt(3+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+3].N#[C-].N([C@@H]([C@]1(C)[N-]\C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C(\C)/C1=N/C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C\C1=N\C([C@H](C1(C)C)CCC(N)=O)=C/1C)[C@@H]2CC(N)=O)=C\1[C@]2(C)CCC(=O)NC[C@@H](C)OP([O-])(=O)O[C@H]1[C@@H](O)[C@@H](N2C3=CC(C)=C(C)C=C3N=C2)O[C@@H]1CO FDJOLVPMNUYSCM-WZHZPDAFSA-L 0.000 claims description 10
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 claims description 10
- 239000000843 powder Substances 0.000 claims description 10
- 229960003495 thiamine Drugs 0.000 claims description 10
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 claims description 10
- 235000019155 vitamin A Nutrition 0.000 claims description 10
- 239000011719 vitamin A Substances 0.000 claims description 10
- 235000010374 vitamin B1 Nutrition 0.000 claims description 10
- 239000011691 vitamin B1 Substances 0.000 claims description 10
- 235000019163 vitamin B12 Nutrition 0.000 claims description 10
- 239000011715 vitamin B12 Substances 0.000 claims description 10
- 235000019165 vitamin E Nutrition 0.000 claims description 10
- 239000011709 vitamin E Substances 0.000 claims description 10
- 229940046009 vitamin E Drugs 0.000 claims description 10
- 229940045997 vitamin a Drugs 0.000 claims description 10
- 244000005700 microbiome Species 0.000 claims description 8
- 229910021536 Zeolite Inorganic materials 0.000 claims description 5
- -1 compound vitamin Chemical class 0.000 claims description 5
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 claims description 5
- 239000010457 zeolite Substances 0.000 claims description 5
- 241000208688 Eucommia Species 0.000 claims description 4
- 229940047169 astragalus root extract Drugs 0.000 claims description 4
- 229940069445 licorice extract Drugs 0.000 claims description 4
- 238000002360 preparation method Methods 0.000 abstract description 13
- 210000003278 egg shell Anatomy 0.000 abstract description 12
- 230000000694 effects Effects 0.000 abstract description 5
- 239000003674 animal food additive Substances 0.000 abstract description 3
- 239000004615 ingredient Substances 0.000 abstract description 3
- 238000003860 storage Methods 0.000 abstract description 3
- 230000002708 enhancing effect Effects 0.000 abstract 1
- 241000194032 Enterococcus faecalis Species 0.000 description 6
- 239000009636 Huang Qi Substances 0.000 description 6
- 229940032049 enterococcus faecalis Drugs 0.000 description 6
- 102000002322 Egg Proteins Human genes 0.000 description 5
- 108010000912 Egg Proteins Proteins 0.000 description 5
- 240000008042 Zea mays Species 0.000 description 5
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 5
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 5
- 235000005822 corn Nutrition 0.000 description 5
- 210000001595 mastoid Anatomy 0.000 description 5
- 244000046052 Phaseolus vulgaris Species 0.000 description 4
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 4
- 239000004575 stone Substances 0.000 description 4
- 230000000052 comparative effect Effects 0.000 description 3
- 229940012466 egg shell membrane Drugs 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- 150000001413 amino acids Chemical class 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 150000003722 vitamin derivatives Chemical class 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 235000008733 Citrus aurantifolia Nutrition 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 235000019733 Fish meal Nutrition 0.000 description 1
- 235000019764 Soybean Meal Nutrition 0.000 description 1
- 235000011941 Tilia x europaea Nutrition 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 230000002301 combined effect Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000004467 fishmeal Substances 0.000 description 1
- 230000012447 hatching Effects 0.000 description 1
- 239000004571 lime Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000004455 soybean meal Substances 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 238000002834 transmittance Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/28—Silicates, e.g. perlites, zeolites or bentonites
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/30—Oligoelements
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Inorganic Chemistry (AREA)
- Molecular Biology (AREA)
- Mycology (AREA)
- Physiology (AREA)
- Botany (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Birds (AREA)
- Feed For Specific Animals (AREA)
- Fodder In General (AREA)
Abstract
The invention provides a biological organic mineral composite additive for reducing dark spot eggs and a use method thereof. The key technology of the invention mainly comprises a preparation method and a using method of the additive. The additive mainly comprises three functional components, including main active components: biological organic trace elements, auxiliary component 1: plant extract, auxiliary ingredient 2: vitamins. The three functional components are combined into the mixed feed additive according to a certain proportion, and added into daily ration of laying hens according to 0.05% -0.3%, so that dark spot rate of eggs laid by the laying hens in the same day and storage for 3d and 5d can be remarkably reduced. In addition, the additive can improve the surface glossiness of eggshells and can obviously reduce the egg breaking rate of eggs laid by laying hens in the later period of laying. Thereby achieving the effects of improving the quality of egg shells and prolonging the storage life and enhancing the purchase intention of consumers.
Description
Technical Field
The invention belongs to the field of feed additives, and particularly relates to a biological organic mineral composite additive for reducing dark spot eggs of eggs and a use method thereof.
Background
Dark spot eggs, also known as watermark eggs, thin spot eggs or spot eggs. Dark spot eggs are mainly produced on egg shells of eggs and other eggs, but affect the quality of the eggs and commercial eggs, so that the hatching performance of the eggs and the quality of the shells of commercial eggs are further affected. Research on the formation mechanism of the dark spot egg shows that the dark spot egg is closely related to the microstructure of the eggshell, the inner eggshell membrane and the outer eggshell membrane of the dark spot egg are thinner, the macropores among fibers are more, the mastoid layers are not tightly connected, the gaps are large, the fiber structure on the inner surface of the lime layer is disordered, loose and the like. The above defect of microstructure makes moisture easily permeate into eggshells and accumulate at loose portions of eggshells, resulting in a difference in light transmittance, forming so-called dark spots. Research has shown that dark spot eggs are the result of the combined effects of a variety of factors, such as poultry variety, age, nutrition, environment, disease, etc., alone or in combination, can affect the incidence of dark spot eggs to varying degrees.
At present, nutrition regulation is one of important measures for reducing the incidence rate of dark spot eggs. Studies have shown that calcium and vitamin D are properly supplemented 3 Can reduce the incidence rate of dark spot eggs to a certain extent. From the viewpoint of nutrients such as amino acids and vitamins, it has been studied that the occurrence of dark spot eggs can be reduced by properly increasing a part of vitamins or balancing certain amino acids. However, the production practice shows that the factors have uncertainty in improving the effect of the dark spot eggs of the eggs, the improvement effect is achieved in a short time, and the occurrence rate of the dark spot eggs is high after long-term addition.
Disclosure of Invention
In order to solve the problems, the invention provides the biological organic mineral composite additive for reducing the dark spot egg of the egg and the use method thereof, which can improve the quality of the eggshell of the egg and prolong the storage life.
In order to achieve the above purpose, the technical scheme adopted by the invention is as follows:
the biological organic mineral composite additive for reducing dark spot eggs of eggs is added into daily ration of laying hens, and is characterized in that: the biological organic mineral composite additive comprises biological organic trace elements, plant extracts and vitamins, and is added into the daily ration of the laying hen according to the weight ratio of 0.05% -0.3%.
Further, the biological organic trace elements are biological compound organic trace elements, the plant extract is a compound plant extract, and the vitamins are compound vitamins.
Further, the biological composite organic trace elements are composite organic trace elements produced by simultaneously fermenting zinc, manganese and copper through trace element resistant microorganisms; the compound plant extract comprises licorice extract, eucommia bark extract and astragalus root extract; the compound vitamins comprise vitamin A, vitamin E, vitamin D, vitamin B1 and vitamin B12.
Further, the composite organic trace elements comprise organic zinc, organic manganese and organic copper, and the content of the organic zinc, the organic manganese and the organic copper is not less than 65.5%; the purity of the licorice extract, the eucommia bark extract and the astragalus root extract in the composite plant extract is not lower than 95 percent; the purity of vitamin A, vitamin E, vitamin D, vitamin B1 and vitamin B12 in the compound vitamin is not lower than 98 percent.
Further, the trace element tolerant microorganisms are subjected to single element concentration gradient domestication and mixed element concentration gradient domestication of zinc, manganese and copper respectively, the gradient concentration of copper comprises 350mg/L, 450mg/L, 550mg/L and 650mg/L, the gradient concentration of zinc comprises 1500mg/L, 1800mg/L, 2100mg/L and 2500mg/L, and the gradient concentration of manganese comprises 1200mg/L, 1500mg/L, 1800mg/L and 2200mg/L.
Further, the bio-organic ore composite additive comprises, by mass, 20 parts of bio-organic trace elements, 8 parts of plant extracts, 1 part of vitamins and 71 parts of zeolite powder.
Further, the daily ration for the laying hens comprises the following components in parts by mass: 55-65 parts of corn, 22-25 parts of soybean meal, 3.5-9 parts of stone powder and 3-5 parts of premix.
A method for using the bio-organic mineral composite additive for reducing dark spot eggs of eggs is provided, wherein the bio-organic mineral composite additive is added in stages according to the egg laying stage of the laying hen.
Further, the staged addition of the bio-organic mineral composite additive includes three stages: the first stage is an initial egg laying stage from an initial egg laying stage to an egg laying rate of 85%, and the adding amount of the bio-organic mineral composite additive in the daily ration of the laying hen in the initial egg laying stage is 0.05% -0.1% by weight; the second stage is an egg laying peak period with the egg laying rate higher than 85%, and the addition amount of the bio-organic mineral composite additive in the daily ration of the laying hen in the egg laying peak period is 0.15% -0.2% by weight; the third stage is the later stage of laying eggs, the laying rate of which is reduced to below 85 percent, and the adding amount of the bio-organic mineral composite additive in the daily ration of the laying hens in the later stage of laying eggs is 0.25-0.3 percent by weight.
The beneficial effects of the invention are as follows: according to the biological organic mineral composite additive for reducing the dark spot of the eggs and the application method, the occurrence of dark spot rate of the eggs can be reduced to a large extent by adding trace element resistant microorganisms and simultaneously fermenting and enriching the composite organic trace elements, and the simultaneous addition of the two auxiliary component composite plant extracts and the composite vitamins can produce a synergistic effect with the composite organic trace elements, so that the dark spot rate of the eggs can be further reduced.
Drawings
FIG. 1 is an eggshell ultrastructural view of eggs laid using the bio-organic mineral composite additive of the present invention;
FIG. 2 is an ultrastructural view of eggshells of eggs laid by inorganic mineral additives;
FIG. 3 is a bar graph of cracked egg rates for 8 sets of comparative experiments;
fig. 4 is a graph of dark spot rates of eggs for different durations of use of the bio-organic mineral composite additive.
Detailed Description
In order that those skilled in the art will better understand the technical solutions provided by the present invention, the following description is made with reference to specific embodiments.
The invention relates to a biological organic mineral composite additive for reducing dark spot eggs of eggs and a use method thereof, which comprises the steps of mixing main active ingredient biological organic trace elements with auxiliary ingredient 1 plant extracts, preparing auxiliary ingredient 2 vitamins into a feed additive according to a certain proportion, and adding the additive into daily ration of laying hens by 0.05% -0.3%.
Example 1
1. Daily ration for laying hens
Taking 64 parts of corn, 23 parts of bean pulp, 8 parts of stone powder and 5 parts of premix according to the parts by weight, and uniformly mixing to obtain the daily ration for the laying hens.
2. Preparation of biological organic ore composite additive
Firstly, domesticating microelement resistant microorganism enterococcus faecalis, namely firstly, performing single element concentration gradient domestication on zinc, manganese and copper respectively, and then performing domestication by adopting a mixed element culture medium of copper (350, 450, 550, 650 mg/L), zinc (1500, 1800, 2100, 2500 mg/L) and manganese (1200, 1500, 1800, 2200 mg/L);
and (3) fermenting the domesticated enterococcus faecalis to obtain composite organic microelements, wherein the content of the organic zinc, the organic manganese and the organic copper in the obtained biological composite organic zinc, manganese and copper is 65.5%.
3. Preparation of plant extracts
Mixing Glycyrrhrizae radix extract, eucommiae cortex extract and radix astragali extract to obtain compound extract, wherein the purity of Glycyrrhrizae radix extract, eucommiae cortex extract and radix astragali extract in the compound plant extract is 95%.
4. Preparation of vitamins
Mixing vitamin A, vitamin E, vitamin D, vitamin B1 and vitamin B12 to obtain compound vitamin, wherein the purity of the vitamin A, vitamin E, vitamin D, vitamin B1 and vitamin B12 is 98%.
5. Preparation of bio-organic ore composite additive
And mixing 20 parts of biological organic trace elements, 8 parts of plant extracts, 1 part of vitamins and 71 parts of zeolite powder according to the mass parts.
6. Application method of bio-organic ore composite additive
The bio-organic mineral composite additive is also added in stages according to the egg laying stage of the laying hen, and comprises three stages: the first stage is an initial egg laying stage from an initial egg laying stage to an egg laying rate of 85%, and the adding amount of the bio-organic mineral composite additive in the daily ration of the laying hen in the initial egg laying stage is 0.05% by weight; the second stage is an egg laying peak period with the egg laying rate higher than 85%, and the addition amount of the bio-organic mineral composite additive in the daily ration of the laying hen in the egg laying peak period is 0.15% by weight; the third stage is the later stage of laying eggs, the laying rate of which is reduced to below 85 percent, and the adding amount of the bio-organic mineral composite additive in the daily ration of the laying hens in the later stage of laying eggs is 0.25 percent by weight.
Example 2
1. Daily ration for laying hens
Taking 65 parts of corn, 25 parts of bean pulp, 7 parts of stone powder and 3 parts of premix according to parts by weight, and uniformly mixing to obtain the daily ration of the laying hen, wherein the daily ration of the laying hen can also be prepared by adopting bran and secondary powder to replace a certain proportion of corn or adopting vegetable pulp, cotton pulp, fish meal and the like to replace a certain proportion of bean pulp, and the replaced daily ration of the laying hen is equal energy and equal protein.
2. Preparation of biological organic ore composite additive
Firstly, domesticating microelement resistant microorganism enterococcus faecalis, namely firstly, performing single element concentration gradient domestication on zinc, manganese and copper respectively, and then performing domestication by adopting a mixed element culture medium of copper (350, 450, 550, 650 mg/L), zinc (1500, 1800, 2100, 2500 mg/L) and manganese (1200, 1500, 1800, 2200 mg/L);
and (3) fermenting the domesticated enterococcus faecalis to obtain composite organic microelements, wherein the content of the organic zinc, the organic manganese and the organic copper in the obtained biological composite organic zinc, manganese and copper is 66%.
3. Preparation of plant extracts
Mixing Glycyrrhrizae radix extract, eucommiae cortex extract and radix astragali extract to obtain compound extract, wherein the purity of Glycyrrhrizae radix extract, eucommiae cortex extract and radix astragali extract in the compound plant extract is 95%.
4. Preparation of vitamins
Mixing vitamin A, vitamin E, vitamin D, vitamin B1 and vitamin B12 to obtain compound vitamin, wherein the purity of the vitamin A, vitamin E, vitamin D, vitamin B1 and vitamin B12 is 98%.
5. Preparation of bio-organic ore composite additive
Mixing 20 parts of biological organic trace elements, 8 parts of plant extracts, 1 part of vitamins and 71 parts of zeolite powder according to mass parts, and adding the mixture into premix of daily ration of laying hens.
6. Application method of bio-organic ore composite additive
The bio-organic mineral composite additive is also added in stages according to the egg laying stage of the laying hen, and comprises three stages: the first stage is an initial egg laying stage from an initial egg laying stage to an egg laying rate of 85%, and the adding amount of the bio-organic mineral composite additive in the daily ration of the laying hen in the initial egg laying stage is 0.08% by weight; the second stage is an egg laying peak period with the egg laying rate higher than 85%, and the addition amount of the bio-organic mineral composite additive in the daily ration of the laying hen in the egg laying peak period is 0.18% by weight; the third stage is the later stage of laying eggs, the laying rate of which is reduced to below 85 percent, and the adding amount of the bio-organic mineral composite additive in the daily ration of the laying hens in the later stage of laying eggs is 0.28 percent by weight.
Example 3
1. Daily ration for laying hens
62 parts of corn, 25 parts of bean pulp, 9 parts of stone powder and 4 parts of premix are taken according to the parts by weight, and the daily ration of the laying hen is prepared after the components are uniformly mixed.
2. Preparation of biological organic ore composite additive
Domestication of microelement resistant microorganism enterococcus faecalis, firstly, performing single element concentration gradient domestication of zinc, manganese and copper respectively, and then performing domestication by adopting a mixed element culture medium of copper (350, 450, 550, 650 mg/L), zinc (1500, 1800, 2100, 2500 mg/L) and manganese (1200, 1500, 1800, 2200 mg/L);
and (3) fermenting the domesticated enterococcus faecalis to obtain composite organic microelements, wherein the content of the organic zinc, the organic manganese and the organic copper in the obtained biological composite organic zinc, manganese and copper is not less than 65.5%.
3. Preparation of plant extracts
Mixing Glycyrrhrizae radix extract, eucommiae cortex extract and radix astragali extract to obtain compound extract, wherein the purity of Glycyrrhrizae radix extract, eucommiae cortex extract and radix astragali extract in the compound plant extract is not less than 95%.
4. Preparation of vitamins
Mixing vitamin A, vitamin E, vitamin D, vitamin B1 and vitamin B12 to obtain compound vitamin, wherein the purity of the vitamin A, vitamin E, vitamin D, vitamin B1 and vitamin B12 is not lower than 98%.
5. Preparation of bio-organic ore composite additive
The biological organic microelement compound is prepared by mixing 20 parts by weight of biological organic microelement, 8 parts by weight of plant extract, 1 part by weight of vitamin and 71 parts by weight of zeolite powder.
6. Application method of bio-organic ore composite additive
The bio-organic mineral composite additive is also added in stages according to the egg laying stage of the laying hen, and comprises three stages: the first stage is an initial egg laying stage from an initial egg laying stage to an egg laying rate of 85%, and the adding amount of the bio-organic mineral composite additive in the daily ration of the laying hen in the initial egg laying stage is 0.1% by weight; the second stage is an egg laying peak period with the egg laying rate higher than 85%, and the addition amount of the bio-organic mineral composite additive in the daily ration of the laying hen in the egg laying peak period is 0.2% by weight; the third stage is the later stage of laying eggs, the laying rate of which is reduced to below 85 percent, and the adding amount of the bio-organic mineral composite additive in the daily ration of the laying hens in the later stage of laying eggs is 0.3 percent by weight.
Example 4
Comparative tests were carried out with the inorganic mineral additives and the bio-organic mineral additives:
selecting the day age of laying hens: 560 days;
selecting a variety of laying hens: sea blue brown, the feeding amount is 15000.
The comparative tests were grouped according to whether or not the inorganic mineral additives, the bio-organic mineral additives, the plant extracts and the vitamins were added in the composite additives: inorganic ore control group 1: adding inorganic ore; inorganic ore control group 2: adding inorganic ore + plant extract; inorganic ore control group 3: adding inorganic ore and vitamin; inorganic ore control group 4: adding inorganic ore, plant extract and vitamins; bio-organic ore control group 1: adding biological organic trace elements; bio-organic ore control group 2: adding biological organic trace elements and plant extracts; bio-organic ore control group 3: adding biological organic trace elements and vitamins; bio-organic ore control group 4: adding biological organic trace elements, plant extracts and vitamins.
FIG. 3 shows crack egg rate of 8 groups of comparison experiments, and compared with all the control groups added with inorganic ore, the biological organic ore control group 1 (only added with biological organic trace elements) can reduce the occurrence of dark spot rate of eggs to a greater extent; the plant extracts and vitamins with two auxiliary components can improve the effect of biological organic trace elements on reducing the dark spot rate, especially, the plant extracts and vitamins have the best effect when added simultaneously, the dark spot rate is only 7.66 percent (biological organic ore control group 4), and 81.19 percent and 55.23 percent are respectively reduced compared with the inorganic ore control group 1 (single inorganic ore) and the biological organic ore control group 1 (single biological organic trace element).
FIG. 1 is an eggshell ultrastructural view of eggs laid using the bio-organic mineral composite additive of the present invention; FIG. 2 is an ultrastructural view of eggshells of eggs laid by inorganic mineral additives; in fig. 2, egg shell membranes in the ultrastructure of eggs produced without the bio-organic mineral composite additive group (inorganic mineral control group 1) are thinner and are not tightly connected with mastoid; in addition, the mastoid space is large, and the structure is loose; the fence layer structure is loose. In contrast, in fig. 1, the eggshell membrane using the bio-organic mineral composite additive group (bio-organic mineral control group 4) is thicker, and is tightly connected with the mastoid layers, and the mastoid is orderly arranged, and has a compact structure and a compact fence layer structure.
FIG. 4 is a graph of egg dark spot rates for various durations of use of the bio-organic mineral composite additive, which shows a decreasing trend over time after use, with the maximum dark spot reducing effect at day 15.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. The present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (2)
1. The biological organic mineral composite additive for reducing dark spot eggs of eggs is added into daily ration of laying hens, and is characterized in that: the biological organic mineral composite additive comprises biological organic trace elements, plant extracts and vitamins, and is added into laying hen daily ration according to the weight ratio of 0.05% -0.3%;
the biological organic trace elements are biological compound organic trace elements, the plant extract is a compound plant extract, and the vitamins are compound vitamins;
the biological composite organic trace elements are composite organic trace elements produced by simultaneously fermenting zinc, manganese and copper through trace element resistant microorganisms; the compound plant extract comprises licorice extract, eucommia bark extract and astragalus root extract; the compound vitamins comprise vitamin A, vitamin E, vitamin D, vitamin B1 and vitamin B12;
the compound organic microelements comprise organic zinc, organic manganese and organic copper, and the content of the organic zinc, the organic manganese and the organic copper is not less than 65.5%; the purity of the licorice extract, the eucommia bark extract and the astragalus root extract in the composite plant extract is not lower than 95 percent; the purity of vitamin A, vitamin E, vitamin D, vitamin B1 and vitamin B12 in the compound vitamin is not lower than 98%;
the biological organic mineral composite additive comprises, by mass, 20 parts of biological organic trace elements, 8 parts of plant extracts, 1 part of vitamins and 71 parts of zeolite powder;
the bio-organic mineral composite additive is added in stages according to the egg laying stage of the laying hen;
the staged addition of the bio-organic mineral complex additive includes three stages: the first stage is an initial egg laying stage from an initial egg laying stage to an egg laying rate of 85%, and the adding amount of the bio-organic mineral composite additive in the daily ration of the laying hen in the initial egg laying stage is 0.05% -0.1% by weight; the second stage is an egg laying peak period with the egg laying rate higher than 85%, and the addition amount of the bio-organic mineral composite additive in the daily ration of the laying hen in the egg laying peak period is 0.15% -0.2% by weight; the third stage is the later stage of laying eggs, the laying rate of which is reduced to below 85 percent, and the adding amount of the bio-organic mineral composite additive in the daily ration of the laying hens in the later stage of laying eggs is 0.25-0.3 percent by weight.
2. The bio-organic mineral composite additive for reducing dark spot eggs of eggs according to claim 1, wherein: the microelement resistant microorganism is subjected to single element concentration gradient domestication and mixed element concentration gradient domestication of zinc, manganese and copper respectively, wherein the gradient concentration of copper comprises 350mg/L, 450mg/L, 550mg/L and 650mg/L, the gradient concentration of zinc comprises 1500mg/L, 1800mg/L, 2100mg/L and 2500mg/L, and the gradient concentration of manganese comprises 1200mg/L, 1500mg/L, 1800mg/L and 2200mg/L.
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| CN109480124A (en) * | 2019-01-02 | 2019-03-19 | 山东省农业科学院家禽研究所 | A method of reducing egg dark line rate |
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| CN102204629A (en) * | 2011-05-19 | 2011-10-05 | 浙江农林大学 | Feed additive and preparation method thereof |
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