CN111280053A - Saline-alkali tolerant barley breeding method - Google Patents
Saline-alkali tolerant barley breeding method Download PDFInfo
- Publication number
- CN111280053A CN111280053A CN202010088248.3A CN202010088248A CN111280053A CN 111280053 A CN111280053 A CN 111280053A CN 202010088248 A CN202010088248 A CN 202010088248A CN 111280053 A CN111280053 A CN 111280053A
- Authority
- CN
- China
- Prior art keywords
- alkali
- saline
- salt
- barley
- identification
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
Abstract
The invention provides a method for breeding saline-alkali tolerant barley, which comprises the following steps: (1) drought resistance identification is carried out on the perennial stable high-generation strain barley, and a drought resistance strain is selected from the drought resistance identification; (2) field identification: carrying out salt and alkali tolerance identification on the drought-resistant product system obtained by screening in the step (1), watering saline-alkali water in a growth period, and harvesting barley seeds with high salt and alkali tolerance in a mature period, wherein the standard of the high salt and alkali tolerance is as follows: only a few plants had mild brown spots on the lower leaves and no dead plants. The invention utilizes the positive correlation of plant drought resistance and salt and alkali resistance to select the salt and alkali resistant barley strain. The saline-alkali tolerant barley strain material suitable for local saline-alkali soil planting is easily selected by field selection of the saline-alkali soil instead of laboratory selection, and the saline-alkali tolerance is not easy to lose.
Description
Technical Field
The invention relates to a method for breeding saline-alkali tolerant barley.
Background
The stress resistance of plants has a plurality of common physiological and ecological mechanisms, drought and salt and alkali are abiotic stress factors, when the plants are stressed by the drought stress or the salt and alkali, the plants can respond to the drought stress or the salt and alkali stress through the change of osmotic substances and the activity change of protective enzyme, and most drought-tolerant plants also have salt tolerance. The saline alkali mainly causes the water absorption difficulty of crops, namely, the physiological drought, but the saline alkali and the drought are not completely the same. Meanwhile, many laboratories in the world acquire saline-alkali tolerant barley varieties under laboratory conditions, but lose the salt tolerance when cultivated in field in saline-alkali soil (Nevo and Chen, 2010).
Disclosure of Invention
In order to solve the problems in the prior art, the invention provides a method for breeding saline-alkali tolerant barley. The invention screens the saline-alkali tolerant barley in the saline-alkali soil for many years, and selects the saline-alkali tolerant barley suitable for local planting.
The invention relates to a method for breeding a new variety of salt and alkali tolerant barley by utilizing the correlation of plant drought resistance and salt and alkali tolerance. The drought resistance and saline-alkali tolerance relativity of the plants is as follows: the stress resistance of plants has a plurality of common physiological and ecological mechanisms, and most of drought-resistant germplasms also have salt tolerance. The saline alkali mainly causes the water absorption difficulty of crops, namely, physiological drought.
The invention provides a method for breeding saline-alkali tolerant barley, which comprises the following steps:
(1) drought resistance identification is carried out on the perennial stable high-generation strain barley, and a drought resistance strain is selected from the drought resistance identification;
(2) field identification: carrying out salt and alkali tolerance identification on the drought-resistant product system obtained by screening in the step (1), pouring saline-alkali water in a growth period, judging the salt and alkali tolerance level, and harvesting barley seeds with high salt and alkali tolerance in a mature period; the standard of high saline-alkali resistance is as follows: only a few lower leaves of the plants have slight brown spots in the growth period, and no dead plants exist.
Preferably, in the step (2), the saline-alkali soil is a local representative saline-alkali soil.
Preferably, in the step (2), the saline-alkali soil is saline-alkali soil of Shandan county, wherein the saline-alkali soil comprises 0.38% of total nitrogen, 0.84% of total phosphorus, 22.4% of total potassium, 8.34% of pH value and 0.77% of total salt.
Preferably, in the step (2), when the saline-alkali water is poured in the growth period, the water quality is as follows: 0.39g/L of chloride ions, 0.54g/L of sulfate ions, 0.050g/L of calcium ions, 0.078g/L of magnesium ions, 1.04g/L of potassium and sodium ions in total, and 2.19g/L, pH of total salt of 7.59.
The method has the beneficial effects that:
1. the invention utilizes the positive correlation of plant drought resistance and salt and alkali resistance to select the salt and alkali resistant barley strain.
2. The drought resistance and the saline-alkali tolerance of the plants are not completely consistent, the saline-alkali tolerance screening is carried out on drought-resistant barley strains, the saline-alkali tolerance barley strains are selected, and meanwhile saline-alkali soil, especially inland saline-alkali soil, is mostly mixed by salinization and alkalization, and the degrees are different and very complex, so that researchers still have to talk about the saline-alkali soil as a whole and are commonly called as the saline-alkali soil.
3. The saline-alkali tolerant barley strain material suitable for local saline-alkali soil planting is easily selected by performing field selection instead of laboratory selection on the saline-alkali soil, and the saline-alkali tolerance is not easy to lose: the saline-alkali resistance is shown in the saline-alkali soil for two years after the saline-alkali soil is continuously planted, so that the saline-alkali resistance is not lost.
Detailed Description
The following examples are given to facilitate a better understanding of the invention, but do not limit the invention. The experimental procedures in the following examples are conventional unless otherwise specified. The test materials used in the following examples are commercially available unless otherwise specified.
Example 1
The saline-alkali tolerant barley breeding method comprises the following steps:
1. and continuously carrying out drought resistance identification on more than 1000 stable high-generation strain materials for many years, and selecting 45 drought-resistant strains from the materials.
2. Field identification: and in 2018 and 2019, saline-alkali tolerance identification is carried out on the 45 drought-resistant strain in saline-alkali soil of Shandan county (wherein in the saline-alkali soil, the total nitrogen is 0.38%, the total phosphorus is 0.84%, the total potassium is 22.4%, the pH value is 8.34, and the total salt is 0.77%). Watering for 3 times in the growth period, wherein the water quality is as follows: 0.39g/L of chloride ions, 0.54g/L of sulfate ions, 0.050g/L of calcium ions, 0.078g/L of magnesium ions, 1.04g/L of potassium and sodium ions in total, and 2.19g/L, pH of total salt of 7.59. Meanwhile, seed reproduction is carried out in non-saline and alkaline land, and later-period test seeds are guaranteed.
The reason for selecting saline-alkali soil in Shandan county is as follows: the saline-alkali soil in Shandan county is a local representative saline-alkali soil in Gansu province.
The test adopts a random block design, repeated for three times, the row length is 1.5m, the row spacing is 0.25m, 5 rows of areas are adopted, 160 grains are sowed in each row, and compared with the strip sowing in the middle and the last ten days of 3 months, the strip sowing is carried out by taking the internationally recognized saline-alkali tolerant barley CM72 as a contrast. The local fertilizing amount is taken as the standard. The growth is observed in each growth period, and the saline-alkali tolerance level is judged.
TABLE 1 grading Standard for field identification of saline-alkali hazard symptoms of crops
3. Seeds of barley with high saline-alkali resistance are harvested in the mature period, and 4 saline-alkali resistant barley strains such as 1056-6, Tibet 89, 0929-1, 0821-2 and the like are obtained.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (4)
1. A method for breeding saline-alkali tolerant barley is characterized by comprising the following steps: the method comprises the following steps:
(1) drought resistance identification is carried out on the perennial stable high-generation strain barley, and a drought resistance strain is selected from the drought resistance identification;
(2) field identification: carrying out salt and alkali tolerance identification on the drought-resistant product system obtained by screening in the step (1), pouring saline-alkali water in a growth period, judging the salt and alkali tolerance level, and harvesting barley seeds with high salt and alkali tolerance in a mature period; the standard of high saline-alkali resistance is as follows: only a few lower leaves of the plants have slight brown spots in the growth period, and no dead plants exist.
2. The method of claim 1, wherein: in the step (2), the saline-alkali soil is a local representative saline-alkali soil.
3. The method of claim 2, wherein: in the step (2), the saline-alkali soil is saline-alkali soil of Shandan county, wherein the saline-alkali soil comprises 0.38% of total nitrogen, 0.84% of total phosphorus, 22.4% of total potassium, 8.34% of pH value and 0.77% of total salt.
4. The method of claim 3, wherein: in the step (2), when saline-alkali water is poured in the growth period, the water quality is as follows: 0.39g/L of chloride ions, 0.54g/L of sulfate ions, 0.050g/L of calcium ions, 0.078g/L of magnesium ions, 1.04g/L of potassium and sodium ions in total, and 2.19g/L, pH of total salt of 7.59.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010088248.3A CN111280053A (en) | 2020-02-12 | 2020-02-12 | Saline-alkali tolerant barley breeding method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010088248.3A CN111280053A (en) | 2020-02-12 | 2020-02-12 | Saline-alkali tolerant barley breeding method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111280053A true CN111280053A (en) | 2020-06-16 |
Family
ID=71017641
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010088248.3A Pending CN111280053A (en) | 2020-02-12 | 2020-02-12 | Saline-alkali tolerant barley breeding method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111280053A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1997013843A1 (en) * | 1995-10-12 | 1997-04-17 | Cornell Research Foundation, Inc. | Production of water stress or salt stress tolerant transgenic cereal plants |
| EP1244349A2 (en) * | 1999-12-22 | 2002-10-02 | BASF Plant Science GmbH | Pyrophosphatase stress-related proteins and methods of use in plants |
| CN1769463A (en) * | 2005-09-22 | 2006-05-10 | 山东大学 | Method for promoting salt and drought tolerance of maize and wheat by combining betA,NHX1,PPase gene and transgene technology |
| CN109169059A (en) * | 2018-08-23 | 2019-01-11 | 新疆农业科学院农作物品种资源研究所 | A kind of winter wheat resisting drought, salt and alkali breeding method |
-
2020
- 2020-02-12 CN CN202010088248.3A patent/CN111280053A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1997013843A1 (en) * | 1995-10-12 | 1997-04-17 | Cornell Research Foundation, Inc. | Production of water stress or salt stress tolerant transgenic cereal plants |
| EP1244349A2 (en) * | 1999-12-22 | 2002-10-02 | BASF Plant Science GmbH | Pyrophosphatase stress-related proteins and methods of use in plants |
| CN1769463A (en) * | 2005-09-22 | 2006-05-10 | 山东大学 | Method for promoting salt and drought tolerance of maize and wheat by combining betA,NHX1,PPase gene and transgene technology |
| CN109169059A (en) * | 2018-08-23 | 2019-01-11 | 新疆农业科学院农作物品种资源研究所 | A kind of winter wheat resisting drought, salt and alkali breeding method |
Non-Patent Citations (5)
| Title |
|---|
| CHENGDAO LI等: "Recent Advances in Breeding Barley for Drought and Saline Stress Tolerance", 《ADVANCES IN MOLECULAR BREEDING TOWARD DROUGHT AND SALT TOLERANT CROPS》 * |
| 乔海龙等: "大麦盐害及耐盐机理的研究进展", 《核农学报》 * |
| 包奇军等: "耐盐碱啤酒大麦鉴定筛选", 《大麦与谷类科学》 * |
| 王春娜等: "盐碱地改良的研究进展", 《防护林科技》 * |
| 马小英等: "干旱和盐胁迫中大麦实时定量PCR内参基因的筛选 ", 《分子植物育种》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Aslam et al. | A rapid screening technique for salt tolerance in rice (Oryza sativa L.) | |
| Okhovatian-Ardakani et al. | Salt tolerance evaluation and relative comparison in cuttings of different omegranate cultivar | |
| Guvenc et al. | Increasing productivity with intercropping systems in cabbage production | |
| Alhassan et al. | Influence of planting densities on the performance of intercropped bambara groundnut with cowpea in Makurdi, Benue state, Nigeria | |
| Tamrat et al. | Effect of plant density on yield components and yield of faba bean (Vicia faba L.) varieties at Wolaita Sodo, Southern Ethiopia | |
| MANNAN et al. | Response of soybean to salinity: II. Growth and yield of some selected genotypes | |
| Haefele et al. | Drought-prone rainfed lowland rice in Asia: limitations and management options | |
| Anoumaa et al. | Characterization of potato (Solanum tuberosum L.) genotypes from the western highlands region of cameroon using morphological and agronomic traits | |
| CN106489637A (en) | A kind of screening technique of alkaline japonica rice variety | |
| Reza et al. | Effect of plant density on morphologic characteristics related to lodging and yield components in different rice varieties (Oriza sativa L.) | |
| CN111280053A (en) | Saline-alkali tolerant barley breeding method | |
| Jefferies | Evaluation of seedling selection for salinity tolerance in potato (Solanum tuberosum L.) | |
| Ofosuhene-Sintim et al. | Evaluation of sesame (Sesamum indicum) production in Ghana | |
| Riaz et al. | Interactive effect of salinity and boron application on growth and physiological traits of sunflower (Helianthus annuus L.) genotypes. | |
| Zhou et al. | Growth and potential reproduction of Poa crymophila in response to season precipitation shortage in the Eastern Tibetan Plateau, China | |
| Shiam et al. | Effect of NaCl salt on vegetative growth and yield of sixteen tomato lines | |
| Fituma et al. | Response of soybean to Bradyrhizobium japonicum inoculation and phosphorus application under intercropping in the Central Rift Valley of Ethiopia | |
| Gikonyo et al. | Rice response to potassium fertilization in Mwea, Kenya. | |
| Taťpinar et al. | Sugar Beet: An Overutilized Ancient Crop | |
| Badillo-Feliciano et al. | Effect of four levels of N, P, K and micronutrients on sweetpotato yields in an oxisol | |
| Mishra | Increasing profitability and resource conservation through paired-row planting geometry in potato | |
| Septar et al. | Effect of the radicular and foliar fertilizer on fruit quality in the peach orchard. | |
| Mengistu et al. | Morphological variability revealed genetic diversity in Shallot (Allium cepa var. aggregatum) segregating populations | |
| Alturaifi et al. | Effects of Low and Moderate Salinity on Zinnia marylandica | |
| HAYILE | EFFECT OF ALTERNATE FURROW IRRIGATION UNDER DIFFERENT MOISTURE REGIMES ON WATER USE EFFICIENCY, GROWTH, YIELD AND YIELD COMPONENTS OF COMMON BEAN (Phaseolus vulgaris L.) AT ALAGE, CENTRAL RIFT VALLEY OF |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |