CN111269835B - Automatic artificial blood vessel culture device - Google Patents
Automatic artificial blood vessel culture device Download PDFInfo
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- CN111269835B CN111269835B CN202010131277.3A CN202010131277A CN111269835B CN 111269835 B CN111269835 B CN 111269835B CN 202010131277 A CN202010131277 A CN 202010131277A CN 111269835 B CN111269835 B CN 111269835B
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/10—Perfusion
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Abstract
The automatic artificial blood vessel culturing device comprises a culturing tank, a first winding connector device and a second winding connector device which are oppositely arranged above the culturing tank, a blood vessel placing frame, a first peristaltic pump, a second peristaltic pump, a first liquid storage bottle and a second liquid storage bottle which are respectively arranged around the culturing tank, and a blood vessel transferring device which is used for transferring a blood vessel to be cultured on the blood vessel placing frame to the first winding connector device and the second winding connector device, wherein the first winding connector device, the first peristaltic pump, the first liquid storage bottle and the second winding connector device are sequentially connected through hoses; one end of the culture tank, the second peristaltic pump, the second liquid storage bottle and the other end of the culture tank are sequentially connected through a hose. The invention designs a double-channel perfusion culture device aiming at vascular tissues, which can culture vascular tissues with different diameters, can automatically grasp the blood vessels, realizes automatic production and reduces pollution to artificial blood vessels.
Description
Technical Field
The invention relates to the technical field of artificial blood vessels, in particular to an automatic artificial blood vessel culture device.
Background
The main means for constructing artificial blood vessels is tissue engineering, i.e. the application of biological and engineering principles to construct new tissue engineering artificial blood vessels. The traditional tissue engineering blood vessel construction method comprises three elements of a bracket, seed cells and a culture device, wherein the cells are planted on the bracket in vitro, and are planted in the body after being cultured for a certain time. Among the culture devices, perfusion culture systems are one of the important means.
Different from the static culture in a general laboratory, the perfusion culture system provides a dynamic culture mode, can simulate the real cell growth environment in a living body, and simulates the flowing effect of blood in blood vessels in a human body, so that on one hand, fresh culture solution is continuously brought into the culture of a reactor, and on the other hand, the culture solution continuously flows out, and the effect of updating the culture medium is achieved.
The culture of vascular tissue differs from that of normal cultures in that an important indicator of vascular tissue success is endothelialization of the blood vessel. During the culturing process, the medium flowing through the outside of the blood vessel is different from the medium flowing through the inside of the blood vessel, and some growth factors which are beneficial to the endothelialization of the blood vessel are often added into the medium flowing through the inside of the blood vessel. The existing culture device generally has only a single culture channel, can not culture vascular tissues pertinently, can not culture vascular tissues with different diameters, and is easy to pollute the blood vessels due to the fact that the blood vessels are fixed by manual intervention in the culture process. Therefore, it is necessary to design an automated device with dual channels that can accommodate different diameter vessel cultures.
Disclosure of Invention
In order to overcome the problems that the existing culture device only has a single culture channel, can not culture vascular tissues pertinently and can not culture vascular tissues with different diameters, and the vascular tissues are usually fixed by manual intervention in the culture process, so that the vascular is easy to pollute, the invention provides an automatic artificial vascular culture device. The invention is improved based on the existing culture device, a double-channel perfusion culture device is designed for vascular tissues, and the perfusion culture device can automatically grasp, connect and fix the vascular tissues, can culture vascular tissues with different diameters, improves the culture efficiency of the vascular, realizes automatic production and further reduces the pollution to artificial blood vessels.
In order to solve the technical problems, the invention adopts the following technical scheme: the automatic artificial blood vessel culturing device comprises a culturing groove, a first winding joint device and a second winding joint device which are oppositely arranged above the culturing groove, a blood vessel placing frame, a first peristaltic pump, a second peristaltic pump, a first liquid storage bottle and a second liquid storage bottle which are respectively arranged around the culturing groove, and a blood vessel transferring device which is used for transferring a blood vessel to be cultured on the blood vessel placing frame to the first winding joint device and the second winding joint device, wherein the first winding joint device is connected with an inlet end of the first peristaltic pump through a hose, an outlet end of the first peristaltic pump is connected with a liquid inlet of the first liquid storage bottle through a hose, and a liquid outlet of the first liquid storage bottle is connected with the second winding joint device through a hose; one end of the culture tank is connected with the inlet end of the second peristaltic pump through a hose, the outlet end of the second peristaltic pump is connected with the liquid inlet of the second liquid storage bottle through a hose, and the liquid outlet of the second liquid storage bottle is connected with the other end of the culture tank through a hose. When the blood vessel is cultured, the blood vessel to be cultured is placed on the blood vessel placing frame, the blood vessel to be cultured on the blood vessel placing frame can be transferred between the first winding connector device and the second winding connector device through the blood vessel transfer device, then two ends of the blood vessel to be cultured are respectively connected with the first winding connector device and the second winding connector device, so that the first winding connector device, the first peristaltic pump, the first liquid storage bottle, the second winding connector device and the blood vessel to be cultured form a first circulation passage, the culture tank, the second peristaltic pump and the second liquid storage bottle form a second circulation passage, culture mediums are contained in the first liquid storage bottle and the second liquid storage bottle, culture mediums flowing through the inside of the blood vessel to be cultured are contained in the first liquid storage bottle, and culture mediums flowing through the outside of the blood vessel to be cultured are contained in the second liquid storage bottle. When in culture, the blood vessel to be cultured on the first winding joint device and the second winding joint device stretches into the culture tank, and the first peristaltic pump and the second peristaltic pump are started to enable culture mediums in the two circulation paths to flow, so that the internal and external simultaneous culture of the blood vessel can be realized.
Further, the first wire winding connector device includes first unipolar motion slip table, be equipped with first cylinder on the slider of first unipolar motion slip table, be connected with the mount base on the piston rod of first cylinder, be equipped with the hose connector mount on the mount base, wear to be equipped with the hose connector on the hose connector mount, hose connector one end pass through the hose with first peristaltic pump entry end is connected, the hose connector other end is used for connecting and waits to cultivate the blood vessel, and when hose connector and waiting to cultivate the blood vessel to be connected, only need its end to be followed and wait to cultivate blood vessel one end direct insertion and can be realized connecting.
Further, the first wire winding connector device further comprises a blood vessel fixing device, the blood vessel fixing device comprises a wire winding wheel fixing frame arranged on the fixing frame base and on one side of the hose connector fixing frame, a wire winding wheel assembly is arranged on the wire winding wheel fixing frame, one end of the wire winding wheel assembly, which is used for connecting a blood vessel to be cultivated, is respectively arranged on the same side of the wire winding wheel fixing frame and one side of the hose connector fixing frame, a wire winding fixing groove is formed in one end of the hose connector, which is used for connecting the blood vessel to be cultivated, the hose connector is rotationally connected with the hose connector fixing frame, a stepping motor is further arranged on the wire winding wheel fixing frame, and a rotating shaft of the stepping motor is connected with one end of the hose through a belt and the hose connector. The hose connector is directly inserted into one end of a blood vessel to be cultivated, and although the connection can be realized, the hose connector is unreliable and easy to fall off in the cultivation process, so that the one end of the blood vessel to be cultivated can be firmly fixed on the hose connector by arranging the blood vessel fixing device, and the hose connector is not easy to fall off. The specific theory of operation of vascular fixing device is, the wire is around wired on the reel subassembly to the line on the reel subassembly is flexible, and the one end of the line on the reel subassembly is fixed on the wire winding fixed slot of hose connector, and after the hose connector inserts the blood vessel of waiting to cultivate, starts step motor, step motor can drive the hose connector rotatory through the belt, because the one end of the line on the reel subassembly is fixed on the wire winding fixed slot, so at the rotatory in-process of hose connector, the line on the reel subassembly can be constantly elongated and twine on the outer wall of the one end of waiting to cultivate blood vessel and hose connector connection, with this firm connection that realizes waiting to cultivate blood vessel and hose connector. After the culture is finished, when the cultured blood vessel is required to be loosened, only the stepping motor is required to be operated reversely, the stepping motor can drive the hose connector to rotate reversely, the wire wound on the outer wall of the blood vessel is loosened, and the wire can be retracted by the winding wheel assembly.
Further, the reel assembly comprises a reel connecting block, one end of the reel connecting block is connected with the reel fixing frame through a connecting shaft, one end of the reel connecting block, which is connected with the connecting shaft, is provided with an adjusting groove used for tightness adjustment and enabling the reel connecting block to rotate relative to the connecting shaft, and the relative position of the reel connecting block and the reel fixing frame can be adjusted through the adjusting groove, so that adjustment is convenient according to actual needs. The wire storage device is characterized in that a wire storage wheel, a tensioning wheel, a first wire guide wheel and a second wire guide wheel are sequentially arranged on the wire winding wheel connecting block from one end close to the connecting shaft to the other end, and a wire end fixing groove is formed in the position, close to the wire storage wheel, of the wire winding wheel connecting block. The wire winding on the reel subassembly is stored on the wire storage wheel, and the one end and the end of a thread fixed slot of line are connected, and its other end is walked around take-up pulley, first wire wheel and second wire wheel in proper order and is finally connected with hose connector's wire winding fixed slot. The tensioning wheel can ensure that the wire between the second wire wheel and the winding fixed groove of the hose connector always maintains certain tension and cannot relax, so that the wire between the second wire wheel and the winding fixed groove of the hose connector can be smoothly wound on the outer wall of a blood vessel to be cultured when the stepping motor is started to drive the hose connector to rotate, and certain winding force is ensured, so that the reliability of fixing the blood vessel to be cultured and the hose connector is ensured.
Further, the second wire-wound joint device has the same structure as the first wire-wound joint device. When the blood vessel is cultured, the hose connector of the first winding connector device and the hose connector of the second winding connector device are respectively inserted into two ends of the blood vessel to be cultured and are fixed through the respective blood vessel fixing devices.
Further, the vascular transfer device comprises a second single-shaft movement sliding table, a second air cylinder, a rotary air cylinder and a sucker, wherein the second air cylinder is arranged on a sliding block of the second single-shaft movement sliding table, the rotary air cylinder is connected to a piston rod of the second air cylinder through a connecting block, and the sucker is connected with the rotary air cylinder.
Further, vascular rack, first peristaltic pump and second peristaltic pump are established and are being close to the position of culture tank one end, first stock solution bottle and second stock solution bottle are established and are being close to the position of culture tank other end, the second unipolar motion slip table is established one side of culture tank and with culture tank parallel arrangement, second unipolar motion slip table one end is close to vascular rack, the other end is close to the culture tank is kept away from vascular rack's one end, the top of first stock solution bottle and second stock solution bottle all is equipped with the air inlet.
Furthermore, the automatic artificial blood vessel culturing device further comprises a main support, wherein an upper bottom plate, a middle bottom plate and a lower bottom plate are sequentially arranged on the main support from top to bottom, the first peristaltic pump, the second peristaltic pump and the blood vessel transferring device are arranged on the lower bottom plate, the culturing groove, the blood vessel placing rack, the first liquid storage bottle and the second liquid storage bottle are all arranged on the middle bottom plate, and the first winding joint device and the second winding joint device are arranged on the upper bottom plate.
Further, the lower parts of the first peristaltic pump and the second peristaltic pump, and the second single-axis movement sliding table of the vascular transfer device are arranged on the lower bottom plate, a first long through hole and a slot hole are respectively arranged on the middle bottom plate and correspond to the second single-axis movement sliding table and the first peristaltic pump and the second peristaltic pump, a second cylinder, a rotary cylinder and a sucking disc of the vascular transfer device penetrate through the first long through hole to extend to the upper part of the middle bottom plate, the upper parts of the first peristaltic pump and the second peristaltic pump penetrate through the slot hole to reach the upper part of the middle bottom plate, a first single-axis movement sliding table of the first winding joint device and the second winding joint device is arranged on the upper bottom plate, a second long through hole is arranged on the upper bottom plate and corresponds to the first single-axis movement sliding table, and a first cylinder, a hose joint and a vascular fixing device of the first winding joint device penetrate through the second long through hole to extend to the upper part of the culture groove.
Further, the vessel placement frame comprises a plurality of vessel placement columns with different diameters which are sequentially arranged in parallel. The blood vessel rack can be used for simultaneously placing blood vessels to be cultured with different diameters, continuous culture of the blood vessels with different diameters is carried out, the blood vessels to be cultured on the first winding connector device and the second winding connector are fixed in a mode of winding wires at the connecting end of the blood vessels to be cultured, and therefore the blood vessels to be cultured with different diameters are also suitable for the fixing mode, and fixing reliability can be guaranteed.
Compared with the prior art, the invention has the following beneficial effects:
the automatic artificial blood vessel culturing device can provide a double-channel culturing runner for blood vessel tissues, one channel flows through the outer wall of the blood vessel, the other channel flows through the inner wall of the blood vessel, and the two culturing channels can respectively set different flow rates, so that the automatic artificial blood vessel culturing device can simulate the physiological environment in a human body and realize the simultaneous culture inside and outside the blood vessel.
The automatic artificial blood vessel culturing device can culture artificial blood vessels with different diameters.
The automatic artificial blood vessel culturing device can automatically grasp, connect and fix the cultured blood vessels, automatically put the cultured blood vessels after culturing, is an automatic device, reduces manual intervention in the blood vessel culturing process, and further reduces pollution to the artificial blood vessels.
Drawings
Fig. 1 is a schematic view of the structure of the operation side of the present invention.
Fig. 2 is a schematic diagram of the overall structure of the present invention.
Fig. 3 is a schematic structural view of a first wire-wound joint device and a second wire-wound joint device according to the present invention.
Fig. 4 is a schematic structural view of a vascular delivery device according to the present invention.
Detailed Description
The drawings are for illustrative purposes only and are not to be construed as limiting the present patent; for the purpose of better illustrating the embodiments, certain elements of the drawings may be omitted, enlarged or reduced and do not represent the actual product dimensions; it will be appreciated by those skilled in the art that certain well-known structures in the drawings and descriptions thereof may be omitted. The positional relationship depicted in the drawings is for illustrative purposes only and is not to be construed as limiting the present patent.
As shown in fig. 1 and 2, an automatic artificial blood vessel culturing device comprises a culturing tank 1, a first winding joint device 2 and a second winding joint device 3 which are oppositely arranged above the culturing tank 1, a blood vessel placing frame 4, a first peristaltic pump 5, a second peristaltic pump 6, a first liquid storage bottle 7 and a second liquid storage bottle 8 which are respectively arranged around the culturing tank 1, and a blood vessel transferring device 9 for transferring blood vessels to be cultured on the blood vessel placing frame 4 to the first winding joint device 2 and the second winding joint device 3, wherein the first winding joint device 2 is connected with an inlet end of the first peristaltic pump 5 through a hose, an outlet end of the first peristaltic pump 5 is connected with a liquid inlet of the first liquid storage bottle 7 through a hose, and a liquid outlet of the first liquid storage bottle 7 is connected with the second winding joint device 3 through a hose; one end of the culture tank 1 is connected with the inlet end of the second peristaltic pump 6 through a hose, the outlet end of the second peristaltic pump 6 is connected with the liquid inlet of the second liquid storage bottle 8 through a hose, and the liquid outlet of the second liquid storage bottle 8 is connected with the other end of the culture tank 1 through a hose. When the blood vessel is cultured, the blood vessel to be cultured is placed on the blood vessel placing frame 4, the blood vessel to be cultured on the blood vessel placing frame 4 can be transferred between the first winding joint device 2 and the second winding joint device 3 through the blood vessel transfer device 9, then two ends of the blood vessel to be cultured are respectively connected with the first winding joint device 2 and the second winding joint device 3, so that the first winding joint device 2, the first peristaltic pump 5, the first liquid storage bottle 7, the second winding joint device 3 and the blood vessel to be cultured form a first circulation passage, the culture tank 1, the second peristaltic pump 6 and the second liquid storage bottle 8 form a second circulation passage, culture mediums are contained in the first liquid storage bottle 7 and the second liquid storage bottle 8, the culture mediums flowing through the inside of the blood vessel to be cultured are contained in the first liquid storage bottle 7, and the culture mediums flowing through the outside of the blood vessel to be cultured are contained in the second liquid storage bottle 8. During culture, the blood vessel to be cultured on the first winding joint device 2 and the second winding joint device 3 stretches into the culture tank 1, and the first peristaltic pump 5 and the second peristaltic pump 6 are started to enable culture mediums in two circulation paths to flow, so that the internal and external culture of the blood vessel can be realized.
As shown in fig. 3, the first winding joint device 2 includes a first single-axis moving sliding table 21, a first cylinder 22 is provided on a sliding block of the first single-axis moving sliding table 21, a fixing frame base 23 is connected to a piston rod of the first cylinder 22, a hose joint fixing frame 24 is provided on the fixing frame base 23, a hose joint 25 is provided on the hose joint fixing frame 24 in a penetrating manner, one end of the hose joint 25 is connected with an inlet end of the first peristaltic pump 5 through a hose, the other end of the hose joint 25 is used for connecting a blood vessel to be cultured, and when the hose joint 25 is connected with the blood vessel to be cultured, connection can be achieved only by directly inserting an end of the hose joint into one end of the blood vessel to be cultured.
As shown in fig. 3, the first wire winding connector device 2 further includes a blood vessel fixing device, the blood vessel fixing device includes a wire winding wheel fixing frame 26 disposed on the fixing frame base 23 and on one side of the hose connector fixing frame 24, a wire winding wheel assembly is disposed on the wire winding wheel fixing frame 26, one end of the wire winding wheel assembly, which is used for connecting a blood vessel to be cultured, of the hose connector 25 is disposed on the same side of the wire winding wheel fixing frame 26 and the hose connector fixing frame 24, a wire winding fixing groove 27 is disposed on one end of the hose connector 25, which is used for connecting the blood vessel to be cultured, the hose connector 25 is rotatably connected with the hose connector fixing frame 24, a stepping motor 28 is disposed on the wire winding wheel fixing frame 26, and a rotating shaft of the stepping motor 28 is connected with one end of the hose through a belt 29 in a transmission manner. The hose connector 25 is directly inserted into one end of the blood vessel to be cultured, but the connection can be realized, but the connection is unreliable and easy to fall off in the culturing process, so the one end of the blood vessel to be cultured can be firmly fixed on the hose connector 25 by arranging the blood vessel fixing device, and the connection is not easy to fall off. The specific working principle of the blood vessel fixing device is that the wire is wound on the reel assembly, the wire on the reel assembly can stretch out and draw back, one end of the wire on the reel assembly is fixed on the wire winding fixing groove 27 of the hose connector 25, when the hose connector 25 is inserted into a blood vessel to be cultivated, the stepping motor 28 is started, the stepping motor 28 drives the hose connector 25 to rotate through the belt 29, and the wire on the reel assembly is continuously stretched and wound on the outer wall of one end connected with the hose connector 25 of the blood vessel to be cultivated in the rotating process of the hose connector 25, so that the firm connection between the blood vessel to be cultivated and the hose connector 25 is realized. After the culturing, when the cultured blood vessel is required to be loosened, the stepping motor 28 is only required to be operated reversely, the stepping motor 28 drives the hose connector 25 to rotate reversely, and the wire wound on the outer wall of the blood vessel is loosened and can be retracted by the winding wheel assembly.
As shown in fig. 3, the reel assembly includes a reel connection block 10, one end of the reel connection block 10 is connected with the reel fixing frame 26 through a connection shaft 11, an end of the reel connection block 10 connected with the connection shaft 11 is provided with an adjusting groove 12 for adjusting tightness and enabling the reel connection block 10 to rotate relative to the connection shaft 11, and the relative position of the reel connection block 10 and the reel fixing frame 26 can be adjusted through the adjusting groove 12, so that the adjustment is convenient according to actual needs. The reel connecting block 10 is provided with a wire storage wheel 13, a tension wheel 14, a first wire guide wheel 15 and a second wire guide wheel 16 in sequence from one end close to the connecting shaft 11 to the other end, and the position, close to the wire storage wheel 13, of the reel connecting block 10 is provided with a wire end fixing groove. The wire on the reel assembly is wound and stored on the wire storage wheel 13, one end of the wire is connected to the wire end fixing groove, and the other end thereof sequentially bypasses the tension wheel 14, the first wire guide wheel 15 and the second wire guide wheel 16 and is finally connected to the wire winding fixing groove 27 of the hose connector 25. The tensioning wheel 14 can ensure that the wire between the second wire guide wheel 16 and the winding fixing groove 27 of the hose connector 25 always maintains certain tension and cannot be loosened, so that when the stepping motor 28 is started to drive the hose connector 25 to rotate, the wire between the second wire guide wheel 16 and the winding fixing groove 27 of the hose connector 25 can be smoothly wound on the outer wall of a blood vessel to be cultured, and certain winding force is ensured, so that the reliability of fixing the blood vessel to be cultured and the hose connector 25 is ensured.
In this embodiment, the second wire-wound joint device 3 has the same structure as the first wire-wound joint device 2. When culturing blood vessels, the hose connector 25 of the first winding connector device 2 and the hose connector 25 of the second winding connector device 3 are respectively inserted into two ends of the blood vessels to be cultured and are fixed by respective blood vessel fixing devices.
As shown in fig. 4, the vascular transferring device 9 includes a second single-axis moving sliding table 91, a second air cylinder 92, a rotating air cylinder 93 and a suction cup 94, wherein the second air cylinder 92 is arranged on a sliding block of the second single-axis moving sliding table 91, the rotating air cylinder 93 is connected to a piston rod of the second air cylinder 92 through a connecting block 95, and the suction cup 94 is connected to the rotating air cylinder 93.
As shown in fig. 1 and 2, the vascular placing frame 4, the first peristaltic pump 5 and the second peristaltic pump 6 are arranged at positions close to one end of the culture tank 1, the first liquid storage bottle 7 and the second liquid storage bottle 8 are arranged at positions close to the other end of the culture tank 1, the second single-shaft moving sliding table 91 is arranged at one side of the culture tank 1 and is parallel to the culture tank 1, one end of the second single-shaft moving sliding table 91 is close to the vascular placing frame 4, the other end of the second single-shaft moving sliding table is close to one end of the culture tank 1, which is far away from the vascular placing frame 4, and air inlets are formed in the tops of the first liquid storage bottle 7 and the second liquid storage bottle 8.
As shown in fig. 1 and 2, the automatic artificial blood vessel culturing apparatus of the present invention further comprises a main support 18, wherein the main support 18 is provided with an upper bottom plate 181, a middle bottom plate 182 and a lower bottom plate 183 from top to bottom in sequence, the first wire winding connector device 2 and the second wire winding connector device 3 are provided on the upper bottom plate 181, the culturing tank 1, the blood vessel placing rack 4, the first liquid storage bottle 7 and the second liquid storage bottle 8 are all provided on the middle bottom plate 182, and the first peristaltic pump 5, the second peristaltic pump 6 and the blood vessel transferring device 9 are provided on the lower bottom plate 183.
As shown in fig. 1 and 2, the lower parts of the first peristaltic pump 5 and the second peristaltic pump 6, and the second single-axis movement sliding table 91 of the vascular transfer device 9 are disposed on the lower bottom plate 183, the positions on the middle bottom plate 182 corresponding to the second single-axis movement sliding table 91 and the first peristaltic pump 5 and the second peristaltic pump 6 are respectively provided with a first long through hole and a slot hole, the second air cylinder 92, the rotary air cylinder 93 and the suction cup 94 of the vascular transfer device 9 penetrate through the first long through hole to extend above the middle bottom plate 182, the upper parts of the first peristaltic pump 5 and the second peristaltic pump 6 penetrate through the slot hole to reach above the middle bottom plate 182, the first single-axis movement sliding table 21 of the first wire winding connector device 2 and the second wire winding connector device 3 are disposed on the upper bottom plate 181, the positions on the upper bottom plate 181 corresponding to the first single-axis movement sliding table 21 are respectively provided with a second long through hole, and the first air cylinder 22, the first vascular connector 2 and the second wire winding connector 3, and the hose connector 25 penetrate through the second long through hole to extend above the culture slot 1.
As shown in fig. 1 and 2, the vessel rest 4 comprises a plurality of vessel rest posts with different diameters which are arranged in parallel in sequence. The blood vessels to be cultured with different diameters can be placed on the blood vessel placing frame 4 at the same time so as to perform continuous culture of the blood vessels with different diameters, and the blood vessels to be cultured on the first winding connector device 2 and the second winding connector are fixed in a mode of winding wires at the connecting ends of the blood vessels to be cultured, so that the blood vessels to be cultured with different diameters are also suitable for the fixing mode, and the fixing reliability can be ensured.
The overall working principle of this embodiment is as follows: the first liquid storage bottle 7 and the second liquid storage bottle 8 respectively contain different culture mediums, the machine is started, the first air cylinder 22 and the second air cylinder 92 are in a retraction state, the first single-shaft movement sliding table 21 of the two winding joint devices moves leftwards and rightwards to enable the two winding joint devices to be mutually far away, the second single-shaft movement sliding table 91 moves leftwards to the leftmost end to reach the position of the blood vessel placing frame 4, the sucking disc 94 is ventilated, the sucking disc 94 sucks blood vessels to be cultivated from the blood vessel placing frame 4, the second air cylinder 92 is sprung up to take the blood vessels to be cultivated out of the blood vessel placing frame 4, the second single-shaft movement sliding table 91 moves rightwards to reach the position of the second peristaltic pump 6, the second air cylinder 92 is retracted, the second single-shaft movement sliding table 91 continues to move rightwards to reach the middle of the two winding joint devices, the rotary cylinder 93 is ventilated, the vessel to be cultivated is rotated by 90 degrees clockwise, the vessel to be cultivated is parallel to the cultivation tank 1, the second cylinder 92 is ventilated and ejected, the first single-shaft movement sliding table 21 of the two winding joint devices moves to enable the two winding joint devices to approach, the hose joints 25 of the two winding joint devices are respectively inserted into two ends of the vessel to be cultivated along with the approach of the two winding joint devices, the stepping motor 28 is electrified and rotated clockwise, the stepping motor 28 drives the hose joints 25 to rotate through the belt 29, the wire rotates along with the winding fixed groove 27, the wire is released on the wire storage wheel 13, and then the wire stretches out and winds on the outer wall of the vessel to be cultivated of the hose joints 25 through the tensioning wheel 14, the first wire guide wheel 15 and the second wire guide wheel 16, so that the vessel to be cultivated is fixed. The sucking disc 94 is deflated, the second air cylinder 92 is retracted, the second single-shaft moving sliding table 91 moves leftwards to reach the second peristaltic pump 6, the first air cylinders 22 of the two winding joint devices are ventilated and slowly ejected out, so that the two hose joints 25 stretch into the culture tank 1 with the blood vessels to be cultured, and at the moment, the two winding wheel connecting blocks 10 are far away from the culture tank 1; the first peristaltic pump 5 and the second peristaltic pump 6 are started to set different flow rates, the second peristaltic pump 6 is connected with a liquid inlet of the second liquid storage bottle 8 through a hose, a liquid outlet of the second liquid storage bottle 8 is connected with one end of the culture tank 1 to flow into the culture tank 1, the other end of the culture tank 1 is connected with the second peristaltic pump 6 through a hose to form an outer culture medium runner, the outer culture medium runner flows through the outer wall of a blood vessel to be cultured, one end of the first peristaltic pump 5 is connected with a liquid inlet of the first liquid storage bottle 7 through a hose, a liquid outlet of the first liquid storage bottle 7 is connected with the blood vessel to be cultured through a hose and a hose connector 25, the blood vessel to be cultured is connected with the other end of the first peristaltic pump 5 through another hose connector 25 and a hose to form a closed loop of an inner culture medium, and the outer culture medium runner flows through the inner wall of blood vessel tissue. After the cultivation is completed, the second single-axis movement sliding table 91 moves rightward to come into the middle of the two winding joint devices, the first air cylinders 22 of the two winding joint devices slowly retract, the two hose joints 25 stretch out of the cultivation groove 1 with cultivated blood vessels, the sucking discs 94 ventilate and suck the cultivated blood vessels, the stepping motor 28 rotates anticlockwise, the stepping motor 28 is connected with one side of the hose joints 25 through the belt 29 and drives the hose joints 25 to rotate reversely, the cultivated blood vessels are not fixed any more, the first single-axis movement sliding table 21 of the two winding joint devices moves to enable the two winding joint devices to be far away from each other, the second air cylinders 92 ventilate and pop out, the second single-axis movement sliding table 91 moves leftward, the sucking discs 94 move leftward with the cultivated blood vessels to the second peristaltic pump 6, the rotary air cylinders 93 ventilate, anticlockwise rotate 90 DEG, the second single-axis movement sliding table 91 continues to the left end to come to the blood vessel placing frame 4, the cultivated blood vessels are aligned with the blood vessel placing frame 4, the second air cylinders 92 retract, and the blood vessels after the cultivation are placed back to the blood vessel placing frame 4, and the cultivation process is completed.
The above process is repeated, the blood vessel transfer device 9 moves to different positions of the blood vessel placing frame 4 to select blood vessels to be cultivated with different diameters, the blood vessels to be cultivated with different diameters are cultivated, and then the cultivated blood vessels are placed to different positions of the blood vessel placing frame 4 after the cultivation is finished according to different pipe diameters of the blood vessels to be cultivated.
It is to be understood that the above examples of the present invention are provided for clarity of illustration only and are not limiting of the embodiments of the present invention. Other variations or modifications of the above teachings will be apparent to those of ordinary skill in the art. It is not necessary here nor is it exhaustive of all embodiments. Any modification, equivalent replacement, improvement, etc. which come within the spirit and principles of the invention are desired to be protected by the following claims.
Claims (8)
1. The automatic artificial blood vessel culturing device is characterized by comprising a culturing groove (1), a first winding connector device (2) and a second winding connector device (3) which are oppositely arranged above the culturing groove (1), a blood vessel placing frame (4), a first peristaltic pump (5), a second peristaltic pump (6), a first liquid storage bottle (7) and a second liquid storage bottle (8) which are respectively arranged around the culturing groove (1), and a blood vessel transferring device (9) which is used for transferring blood vessels to be cultured on the blood vessel placing frame (4) to the first winding connector device (2) and the second winding connector device (3), wherein the first winding connector device (2) is connected with an inlet end of the first peristaltic pump (5) through a hose, an outlet end of the first peristaltic pump (5) is connected with a liquid inlet of the first liquid storage bottle (7) through a hose, and a liquid outlet of the first liquid storage bottle (7) is connected with the second winding connector device (3) through a hose; one end of the culture tank (1) is connected with the inlet end of the second peristaltic pump (6) through a hose, the outlet end of the second peristaltic pump (6) is connected with the liquid inlet of the second liquid storage bottle (8) through a hose, and the liquid outlet of the second liquid storage bottle (8) is connected with the other end of the culture tank (1) through a hose;
the first winding joint device (2) and the second winding joint device (3) are respectively provided with a winding wheel assembly and a rotatable hose joint (25); the hose connector (25) is used for being connected with one end of a blood vessel to be cultured, a winding fixing groove (27) is formed in the end of the blood vessel to be cultured, the winding wheel assembly comprises a wire storage wheel (13), a telescopic wire is arranged on the wire storage wheel (13), and one end of the telescopic wire is fixed on the winding fixing groove (27);
the first winding joint device (2) comprises a first single-shaft movement sliding table (21), a first air cylinder (22) is arranged on a sliding block of the first single-shaft movement sliding table (21), a fixing frame base (23) is connected to a piston rod of the first air cylinder (22), a hose joint fixing frame (24) is arranged on the fixing frame base (23), a hose joint (25) is arranged on the hose joint fixing frame (24) in a penetrating mode, one end of the hose joint (25) is connected with an inlet end of the first peristaltic pump (5) through a hose, and the other end of the hose joint (25) is used for connecting a blood vessel to be cultivated;
the first wire winding connector device (2) further comprises a blood vessel fixing device, the blood vessel fixing device comprises a wire winding wheel fixing frame (26) arranged on one side of a hose connector fixing frame (24) on a fixing frame base (23), a wire winding wheel assembly is arranged on the wire winding wheel fixing frame (26), one end of the wire winding wheel assembly, which is used for connecting a blood vessel to be cultivated, is respectively arranged on the same side of the wire winding wheel fixing frame (26) and the hose connector fixing frame (24), the hose connector (25) is rotationally connected with the hose connector fixing frame (24), a stepping motor (28) is further arranged on the wire winding wheel fixing frame (26), and a rotating shaft of the stepping motor (28) is connected with one end of the hose connector (25) through a belt (29) in a transmission mode.
2. The automatic artificial blood vessel culturing apparatus according to claim 1, wherein the reel assembly comprises a reel connecting block (10), one end of the reel connecting block (10) is connected with the reel fixing frame (26) through a connecting shaft (11), one end of the reel connecting block (10) connected with the connecting shaft (11) is provided with an adjusting groove (12) for adjusting tightness and enabling the reel connecting block (10) to rotate relative to the connecting shaft (11), a wire storage wheel (13), a tensioning wheel (14), a first wire guide wheel (15) and a second wire guide wheel (16) are sequentially arranged on the reel connecting block (10) from one end close to the connecting shaft (11) to the other end, and a wire end fixing groove (17) is formed in a position, close to the wire storage wheel (13), of the reel connecting block (10).
3. An artificial vascular automation culture device according to claim 1 or 2, wherein the second wire-wound connector device (3) has the same structure as the first wire-wound connector device (2).
4. An artificial vascular automated culture apparatus according to claim 3, wherein the vascular transfer device (9) comprises a second single-axis movement slipway (91), a second cylinder (92), a rotary cylinder (93) and a suction cup (94), the second cylinder (92) is arranged on the slide block of the second single-axis movement slipway (91), the rotary cylinder (93) is connected to the piston rod of the second cylinder (92) through a connecting block (95), and the suction cup (94) is connected to the rotary cylinder (93).
5. The automatic artificial blood vessel culturing device according to claim 4, wherein the blood vessel placing frame (4), the first peristaltic pump (5) and the second peristaltic pump (6) are arranged at positions close to one end of the culturing groove (1), the first liquid storage bottle (7) and the second liquid storage bottle (8) are arranged at positions close to the other end of the culturing groove (1), the second single-shaft movement sliding table (91) is arranged on one side of the culturing groove (1) and is parallel to the culturing groove (1), one end of the second single-shaft movement sliding table (91) is close to the blood vessel placing frame (4), the other end of the second single-shaft movement sliding table is close to one end of the culturing groove (1) away from the blood vessel placing frame (4), and air inlets are formed in the tops of the first liquid storage bottle (7) and the second liquid storage bottle (8).
6. The automated artificial blood vessel culturing device according to claim 5, further comprising a main support (18), wherein the main support (18) is provided with an upper base plate (181), a middle base plate (182) and a lower base plate (183) from top to bottom in sequence, the first winding joint device (2) and the second winding joint device (3) are arranged on the upper base plate (181), the culturing tank (1), the blood vessel placing rack (4), the first liquid storage bottle (7) and the second liquid storage bottle (8) are all arranged on the middle base plate (182), and the first peristaltic pump (5), the second peristaltic pump (6) and the blood vessel transferring device (9) are arranged on the lower base plate (183).
7. The automated artificial vascular culture device according to claim 6, wherein the first peristaltic pump (5) and the second peristaltic pump (6) are arranged at the lower part of the vascular transfer device (9), the second monoaxial movement sliding table (91) of the vascular transfer device (9) is arranged on the lower bottom plate (183), the first monoaxial movement sliding table (21) of the first wound wire connector device (2) and the second wound wire connector device (3) are respectively provided with a first long through hole and a slot hole at positions corresponding to the second monoaxial movement sliding table (91) and the first peristaltic pump (5) and the second peristaltic pump (6), the second air cylinder (92), the rotary air cylinder (93) and the suction cup (94) of the vascular transfer device (9) penetrate through the first long through hole to extend above the middle bottom plate (182), the upper parts of the first peristaltic pump (5) and the second peristaltic pump (6) penetrate through the slot hole to reach above the middle bottom plate (182), the first monoaxial movement sliding table (21) of the first wound wire connector device (2) and the second wound wire connector device (3) are arranged on the upper bottom plate (182), the first monoaxial movement sliding table (21) of the first wound wire connector device (2) and the second wound wire connector device (3) are respectively provided with a first long through hole and a second winding device (181) corresponding to the first wound wire connector device (2) and the first wound wire connector device (2) The hose connector (25) and the blood vessel fixing device penetrate through the second long through hole and extend to the upper part of the culture tank (1).
8. An automated artificial vascular culture device according to claim 1, wherein the vascular placement frame (4) comprises a plurality of vascular placement columns of different diameters arranged side by side in sequence.
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| US8278101B2 (en) * | 2009-12-07 | 2012-10-02 | Synthecon, Inc. | Stem cell bioprocessing and cell expansion |
| US9932559B2 (en) * | 2012-11-16 | 2018-04-03 | The Johns Hopkins University | Platform for creating an artificial blood brain barrier |
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| US6210591B1 (en) * | 1994-09-16 | 2001-04-03 | Transonic Systems, Inc. | Method to measure blood flow rate in hemodialysis shunts |
| CN101333498A (en) * | 2008-06-13 | 2008-12-31 | 东华大学 | Apparatus for culturing tissue engineered blood |
| JP2016131551A (en) * | 2015-01-22 | 2016-07-25 | 株式会社東海ヒット | Method for perfusing fluid in vascular bed, and apparatus for conducting the method |
| EP3398774A1 (en) * | 2015-12-30 | 2018-11-07 | Revotek Co., Ltd | Printing module for biological printer, and biological printer |
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