CN111265547A - Fullerene and fullerene derivative-containing disinfection spray and preparation method thereof - Google Patents
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Abstract
The invention relates to a disinfection spray containing fullerene and fullerene derivatives and a preparation method thereof, belonging to the technical field of medicines. The disinfectant spray comprises at least one of fullerene and fullerene derivatives, and the preparation method comprises the following steps: s100, proportioning, namely weighing the required fullerene and derivatives thereof and water for injection for later use; s200, mixing materials, namely adding the fullerene and the derivatives thereof weighed in the step S100 and water for injection into a dissolving tank; s300, heating, namely heating the dissolving tank mixed in the step S200; s400, preserving heat, namely preserving heat and stirring the dissolving tank heated in the step S300; and S500, cooling, namely cooling the dissolving tank subjected to heat preservation and stirring in the step S400 to obtain the disinfectant spray.
Description
Technical Field
The invention belongs to the technical field of medicines, and particularly relates to a disinfection spray containing fullerene and fullerene derivatives and a preparation method thereof.
Background
With the increase of urban population density and the use of air conditioners, dust collectors and the like, air pollution is becoming more serious, and diseases caused by biological aerosol are increasing.
The pathogenic components in the biological aerosol mainly comprise active virus, active or dormant bacteria and fungi (spores), the particle size of the components is usually small (the particle size of the virus can be less than 100nm, the particle size of the bacteria is mainly 0.25-10 μm, and the particle size of the fungi spores is mainly 1-30 μm), and the components can directly reach the deep lung of a human body through the respiratory action of the human body, so that the occurrence of lung diseases is caused, the risk of related diseases is increased, and even infectious diseases are caused.
In addition, these bioaerosols can be airborne for hundreds of meters or even thousands of meters, thereby spreading to various corners of public places, greatly increasing their hazard range.
SARS virus, once world-abused, is typically an infectious virus that is transmitted in aerosol form. Thereafter, epidemic infectious diseases such as avian influenza, influenza A, hand-foot-and-mouth disease and the like which are transmitted through aerosol appear at home and abroad in succession, and the health of the masses is seriously harmed.
Besides direct source contact with aerosol through mucous membrane or skin of people, the biological aerosol also enters human body mainly through modes of respiratory tract inhalation, digestive tract ingestion and the like, and damages the health of susceptible people.
At present, clinically, the infection caused by the biological aerosol is mainly treated by singly using antibacterial or antiviral drugs, no specific drug which can resist viruses and bacteria exists in the market, and most drugs need to play a treatment role through a blood system and need a certain onset time.
Fullerene (Fullerene) is a hollow molecule composed entirely of carbon and is in the shape of a sphere, ellipsoid, column or tube. Has found C44、C50、C60、C76、C80、C84、C90、C94、C120、C180、C540Etc. of pure carbon. Due to the special structure and properties, fullerenes exhibit excellent properties in superconducting, magnetic, optical, catalytic, material, and biological aspects. In recent years, many biological functions of fullerene have been discovered, and the fullerene has activities of antioxidant activity, cytoprotective activity, antiviral activity, drug-carrying activity, tumor treatment and the like, and has great potential in the field of biomedicine. However, the fullerene used alone for sterilization and virus resistance has not been effective so far, and it is difficult to completely kill bacteria and viruses.
The spray is powered by compressed air, and has a particle size below 60 μm, and can directly act on skin surface and mucosa of cavity and tract, and directly reach deep lung after inhalation, and can directly kill microorganisms.
Disclosure of Invention
1. Technical problem to be solved by the invention
The invention aims to solve the problem that the existing disinfectant spray is difficult to rapidly kill microorganisms.
2. Technical scheme
In order to achieve the purpose, the technical scheme provided by the invention is as follows:
the invention relates to a disinfection spray containing fullerene and fullerene derivatives, which comprises at least one of fullerene and fullerene derivatives.
Preferably, the mass fraction of the fullerene and the fullerene derivative is 0.01-10%.
Preferably, the fullerene-containing compound and the derivative thereof are C60Fullerene and its derivative, C70At least one of fullerene and its derivative.
Preferably, the composition also comprises cetylpyridinium chloride and hydroxyl alcohol compounds.
Preferably, the mass fraction of the cetylpyridinium chloride is 0.01-1.0%, and the mass fraction of the hydroxyl alcohol compound is 0.1-50%.
Preferably, the hydroxyl alcohol compound is at least one selected from the group consisting of ethanol, glycerol, 1, 3-propanediol, 1, 2-propanediol, 1, 3-butanediol, sorbitol, 1, 2-pentanediol, methylpropanediol, 1, 2-hexanediol, 1, 2-octanediol, 1, 2-decanediol, 1, 5-pentanediol, 1, 6-hexanediol, 1, 8-octanediol, and 1, 2-decanediol.
A method for preparing a disinfectant spray containing fullerene and fullerene derivatives comprises the following steps:
s100, proportioning, namely weighing the required fullerene and derivatives thereof and water for injection for later use;
s200, mixing materials, namely adding the fullerene and the derivatives thereof weighed in the step S100 and water for injection into a dissolving tank;
s300, heating, namely heating the dissolving tank mixed in the step S200;
s400, preserving heat, namely preserving heat and stirring the dissolving tank heated in the step S300;
and S500, cooling, namely cooling the dissolving tank subjected to heat preservation and stirring in the step S400 to obtain the disinfectant spray.
Preferably, the heating temperature in the step S300 is 70-80 ℃, the heating rate is 5-8 ℃/min, and the heat preservation time in the step S400 is 20-40 min.
Preferably, the cooling mode in the step S500 is air cooling or water cooling at a temperature of 20-40 ℃ and a cooling rate of 3-5 ℃/min.
Preferably, the step S100 further includes weighing cetylpyridinium chloride and a hydroxyl alcohol compound in a required mass fraction, and the step S200 further includes adding the weighed cetylpyridinium chloride and the hydroxyl alcohol compound.
3. Advantageous effects
Compared with the prior art, the technical scheme provided by the invention has the following beneficial effects:
the invention relates to a disinfection spray containing fullerene and derivatives thereof and a preparation method thereof, wherein the disinfection spray contains at least one of fullerene and derivatives thereof, and the preparation method comprises the following steps: s100, proportioning, namely weighing the required fullerene and derivatives thereof and water for injection for later use; s200, mixing materials, namely adding the fullerene and the derivatives thereof weighed in the step S100 and water for injection into a dissolving tank; s300, heating, namely heating the dissolving tank mixed in the step S200; s400, preserving heat, namely preserving heat and stirring the dissolving tank heated in the step S300; and S500, cooling, namely cooling the dissolving tank subjected to heat preservation and stirring in the step S400 to obtain the disinfectant spray.
Drawings
FIG. 1 is a flow chart of a method of preparing a disinfectant spray containing fullerene and derivatives thereof according to the present invention;
FIG. 2 is a graph showing the results of a sterilization test of Escherichia coli using the disinfectant spray according to the present invention;
FIG. 3 is a graph showing the results of the sterilization test of the disinfectant spray according to the present invention against Candida albicans;
FIG. 4 is a graph showing the results of an antiviral test using the disinfectant spray of the present invention.
Detailed Description
In order to facilitate an understanding of the invention, the invention will now be described more fully hereinafter with reference to the accompanying drawings, in which several embodiments of the invention are shown, but which may be embodied in many different forms and are not limited to the embodiments described herein, but rather are provided for the purpose of providing a more thorough disclosure of the invention.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs; the terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention; as used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.
Example 1
Referring to fig. 1, the disinfectant spray containing fullerene and fullerene derivatives according to the present embodiment is prepared by the following steps,
s100, preparing materials, namely weighing fullerol (n is 24), cetylpyridinium chloride, 1, 3-butanediol and water for injection for later use, wherein the mass fraction of the fullerol is 0.1%, the mass fraction of the cetylpyridinium chloride is 0.1%, the mass fraction of the 1, 3-butanediol is 20%, and the mass fraction of the water for injection is 79.8%;
s200, mixing materials, namely adding the water for injection and the 1, 3-butanediol weighed in the step S100 into a dissolving tank, stirring, and adding the fullerol into the dissolving tank;
s300, heating, namely heating the dissolving tank mixed in the step S200 to 70-80 ℃, wherein the heating rate is 6 ℃/min;
s400, preserving heat, namely preserving heat and stirring the dissolving tank heated in the step S300, stirring and preserving heat for 30 min;
and S500, cooling, namely cooling the dissolving tank subjected to heat preservation and stirring in the step S400 to 40 ℃, wherein the cooling rate is 3 ℃/min, adding the weighed cetylpyridinium chloride, and uniformly stirring to obtain the disinfection spray.
Example 2
Referring to fig. 1, the disinfectant spray containing fullerene and fullerene derivatives according to the present embodiment is prepared by the following steps,
s100, preparing materials, namely weighing fullerol (n is 24), cetylpyridinium chloride, 1, 3-butanediol and water for injection for later use, wherein the mass fraction of the fullerol is 0.5%, the mass fraction of the cetylpyridinium chloride is 0.1%, the mass fraction of the 1, 3-butanediol is 20%, and the mass fraction of the water for injection is 79.4%;
s200, mixing materials, namely adding the water for injection and the 1, 3-butanediol weighed in the step S100 into a dissolving tank, stirring, and adding the fullerol into the dissolving tank;
s300, heating, namely heating the dissolving tank mixed in the step S200 to 70-80 ℃, wherein the heating rate is 6 ℃/min;
s400, preserving heat, namely preserving heat and stirring the dissolving tank heated in the step S300, stirring and preserving heat for 30 min;
and S500, cooling, namely cooling the dissolving tank subjected to heat preservation and stirring in the step S400 to 40 ℃, wherein the cooling rate is 3 ℃/min, adding the weighed cetylpyridinium chloride, and uniformly stirring to obtain the disinfection spray.
Example 3
Referring to fig. 1, the disinfectant spray containing fullerene and fullerene derivatives according to the present embodiment is prepared by the following steps,
s100, preparing materials, namely weighing fullerol (n is 24), cetylpyridinium chloride, 1, 3-butanediol and water for injection for later use, wherein the mass fraction of the fullerol is 1%, the mass fraction of the cetylpyridinium chloride is 0.1%, the mass fraction of the 1, 3-butanediol is 20%, and the mass fraction of the water for injection is 78.9%;
s200, mixing materials, namely adding the water for injection and the 1, 3-butanediol weighed in the step S100 into a dissolving tank, stirring, and adding the fullerol into the dissolving tank;
s300, heating, namely heating the dissolving tank mixed in the step S200 to 70-80 ℃, wherein the heating rate is 6 ℃/min;
s400, preserving heat, namely preserving heat and stirring the dissolving tank heated in the step S300, stirring and preserving heat for 30 min;
and S500, cooling, namely cooling the dissolving tank subjected to heat preservation and stirring in the step S400 to 40 ℃, wherein the cooling rate is 3 ℃/min, adding the weighed cetylpyridinium chloride, and uniformly stirring to obtain the disinfection spray.
Comparative example 1
Referring to fig. 1, a disinfectant spray containing fullerene and fullerene derivatives according to the present comparative example is prepared by the following steps,
s100, preparing materials, namely weighing 0.1 mass percent of fullerol, 20 mass percent of 1, 3-butanediol and 79.9 mass percent of water for injection for later use;
s200, mixing materials, namely adding the water for injection and the 1, 3-butanediol weighed in the step S100 into a dissolving tank, stirring, and adding the fullerol into the dissolving tank;
s300, heating, namely heating the dissolving tank mixed in the step S200 to 70-80 ℃, wherein the heating rate is 6 ℃/min;
s400, preserving heat, namely preserving heat and stirring the dissolving tank heated in the step S300, stirring and preserving heat for 30 min;
and S500, cooling, namely cooling the dissolving tank subjected to heat preservation and stirring in the step S400 to 40 ℃, wherein the cooling rate is 3 ℃/min, and thus the disinfectant spray is obtained.
Comparative example 2
Referring to fig. 1, a disinfectant spray containing fullerene and fullerene derivatives according to the present comparative example is prepared by the following steps,
s100, preparing materials, namely weighing 0.5 mass percent of fullerol, 20 mass percent of 1, 3-butanediol and 79.5 mass percent of water for injection for later use;
s200, mixing materials, namely adding the water for injection and the 1, 3-butanediol weighed in the step S100 into a dissolving tank, stirring, and adding the fullerol into the dissolving tank;
s300, heating, namely heating the dissolving tank mixed in the step S200 to 70-80 ℃, wherein the heating rate is 6 ℃/min;
s400, preserving heat, namely preserving heat and stirring the dissolving tank heated in the step S300, stirring and preserving heat for 30 min;
and S500, cooling, namely cooling the dissolving tank subjected to heat preservation and stirring in the step S400 to 40 ℃, wherein the cooling rate is 3 ℃/min, and thus the disinfectant spray is obtained.
Comparative example 3
Referring to fig. 1, a disinfectant spray containing fullerene and fullerene derivatives according to the present comparative example is prepared by the following steps,
s100, preparing materials, namely weighing fullerol (n is 24), 1, 3-butanediol and water for injection for later use, wherein the mass fraction of the fullerol is 1%, the mass fraction of the 1, 3-butanediol is 20%, and the mass fraction of the water for injection is 79%;
s200, mixing materials, namely adding the water for injection and the 1, 3-butanediol weighed in the step S100 into a dissolving tank, stirring, and adding the fullerol into the dissolving tank;
s300, heating, namely heating the dissolving tank mixed in the step S200 to 70-80 ℃, wherein the heating rate is 6 ℃/min;
s400, preserving heat, namely preserving heat and stirring the dissolving tank heated in the step S300, stirring and preserving heat for 30 min;
and S500, cooling, namely cooling the dissolving tank subjected to heat preservation and stirring in the step S400 to 40 ℃, wherein the cooling rate is 3 ℃/min, and thus the disinfectant spray is obtained.
Comparative example 4
The disinfection spray of the comparative example is prepared by the following steps,
s100, preparing materials, namely cetylpyridinium chloride, 1, 3-butanediol and water for injection for later use, wherein the mass fraction of the cetylpyridinium chloride is 0.1%, the mass fraction of the 1, 3-butanediol is 20%, and the mass fraction of the water for injection is 79.9%;
s200, mixing materials, namely adding the water for injection and the 1, 3-butanediol weighed in the step S100 into a dissolving tank and stirring;
s300, heating, namely heating the dissolving tank mixed in the step S200 to 70-80 ℃, wherein the heating rate is 6 ℃/min;
s400, preserving heat, namely preserving heat and stirring the dissolving tank heated in the step S300, stirring and preserving heat for 30 min;
and S500, cooling, namely cooling the dissolving tank subjected to heat preservation and stirring in the step S400 to 40 ℃, wherein the cooling rate is 3 ℃/min, adding the weighed cetylpyridinium chloride, and uniformly stirring to obtain the disinfection spray.
And (3) sterilization test:
the activity of the test in sterilization is carried out with reference to the 2008 edition of the Disinfection technical Specification of the Ministry of health. The disinfectant spray prepared in the embodiment 1-3 and the disinfectant spray prepared in the comparative example 1-4 are used as test samples, and test strains are escherichia coli and candida albicans. Specifically, a bacterial suspension having a bacterial content of about 2X 108cfu/mL to 1X 109cfu/mL was prepared, and all of the bacterial liquid for the test and the test sample solution were diluted by 10 times, quickly mixed, and immediately measured. And (3) immediately adding 2.4mL of neutralizer to the mixture after the test bacteria and the disinfectant interact for 1 hour, quickly mixing the mixture, sucking 0.5mL of mixed solution, adding the mixed solution into 4.5mL of Letheen neutralizer, and quickly mixing the mixture by using an electric mixer. 0.1mL of the above solution was inoculated into a petri dish as a test group sample. Meanwhile, phosphate buffer solution is used for replacing disinfectant, serial 10-fold dilution is carried out, and a proper dilution is selected for parallel test to be used as a bacteria count control group sample. All test samples are cultured in a constant temperature incubator at 37 ℃, the test is repeated for 3 times, the number of live bacteria of each group is calculated, then the sterilization rate is calculated, the calculation result is shown in attached figures 2 and 3, and the result shows that the sterilization spray containing the fullerene and the derivatives thereof has obvious sterilization effect on Escherichia coli and Candida albicans. Among them, cetylpyridinium chloride belongs to a cationic surfactant containing nitrogen, can inhibit and kill bacteria by reducing surface tension, but has limited killing effect on fungi, and has problems of association, sedimentation and the like with other anionic substances, thus affecting the use. The hydroxyl alcohol compound is an antibacterial agent with a long history, has a synergistic effect when being used with other antibacterial agents, can also be used as a humectant to increase the use feeling of a product, and cannot play an antibacterial role when being used alone at a lower concentration.
And (3) antiviral test:
the activity of the test in the aspect of antivirus is that the activity of the test sample for inhibiting the infection and the replication of the influenza virus at the cellular level is detected by using the disinfection spray prepared in examples 1 to 3 and the disinfection spray prepared in comparative examples 1 to 4 as test samples through an experimental system for infecting host cells (MDCK cells) by the influenza A virus (H3N 2). As an outline of the experiment, MDCK cells were seeded at a density of 1X 105/ml in 100. mu.L in a 96-well cell culture plate and cultured overnight in an incubator at 37 ℃ under 5% CO2 and saturated humidity. The medium in the 96-well plate was aspirated, and the medium was changed to 100. mu.L of a medium containing 100. mu. mol/L of the test sample, and cultured in a 37 ℃ cell culture chamber. The same concentration of DMSO vehicle in which the drug was dissolved was used as the virus control group. After 2 hours of culture, infecting MDCK cells by using virus-containing liquid, continuously culturing for 96 hours, respectively extracting total RNA in the cells, determining the content of RNA in the cells by using RT-PCR, comparing with the RNA level of a virus control group, calculating the inhibition rate of a test sample on viruses, and the calculation result is shown in figure 4.
The above-mentioned embodiments only express a certain implementation mode of the present invention, and the description thereof is specific and detailed, but not construed as limiting the scope of the present invention; it should be noted that, for those skilled in the art, without departing from the concept of the present invention, several variations and modifications can be made, which are within the protection scope of the present invention; therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (10)
1. A disinfectant spray containing fullerene and derivatives thereof is characterized in that: the disinfectant spray comprises at least one of fullerene and derivatives thereof.
2. A nebuliser according to claim 1, characterised in that: the mass fraction of the fullerene and the fullerene derivatives is 0.01-10%.
3. A nebuliser according to claim 1, characterised in that: the fullerene-containing compound and the derivative thereof are C60Fullerene and its derivative, C70At least one of fullerene and its derivative.
4. A nebuliser according to claim 1, characterised in that: also comprises cetylpyridinium chloride and hydroxyl alcohol compounds.
5. A nebuliser according to claim 4, characterised in that: the mass fraction of the cetylpyridinium chloride is 0.01-1.0%, and the mass fraction of the hydroxyl alcohol compound is 0.1-50%.
6. A nebuliser according to claim 4, characterised in that: the hydroxyl alcohol compound is at least one selected from ethanol, glycerol, 1, 3-propanediol, 1, 2-propanediol, 1, 3-butanediol, sorbitol, 1, 2-pentanediol, methyl propanediol, 1, 2-hexanediol, 1, 2-octanediol, 1, 2-decanediol, 1, 5-pentanediol, 1, 6-hexanediol, 1, 8-octanediol and 1, 2-decanediol.
7. A method for preparing a disinfection spray containing fullerene and derivatives thereof is characterized in that: the method comprises the following steps:
s100, proportioning, namely weighing the required fullerene and derivatives thereof and water for injection for later use;
s200, mixing materials, namely adding the fullerene and the derivatives thereof weighed in the step S100 and water for injection into a dissolving tank;
s300, heating, namely heating the dissolving tank mixed in the step S200;
s400, preserving heat, namely preserving heat and stirring the dissolving tank heated in the step S300;
and S500, cooling, namely cooling the dissolving tank subjected to heat preservation and stirring in the step S400 to obtain the disinfectant spray.
8. The method for preparing a disinfectant spray containing fullerene and fullerene derivatives according to claim 7, wherein the method comprises the following steps: the heating temperature in the step S300 is 70-80 ℃, the heating rate is 5-8 ℃/min, and the heat preservation time in the step S400 is 20-40 min.
9. The method for preparing a disinfectant spray containing fullerene and fullerene derivatives according to claim 7, wherein the method comprises the following steps: the cooling mode in the step S500 is air cooling or water cooling, the temperature is 20-40 ℃, and the cooling rate is 3-5 ℃/min.
10. The method for preparing a disinfectant spray containing fullerene and fullerene derivatives according to claim 7, wherein the method comprises the following steps: step S100 also comprises weighing cetylpyridinium chloride and hydroxyl alcohol compounds with required mass fractions, and step S200 also comprises adding the weighed cetylpyridinium chloride and hydroxyl alcohol compounds.
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Application publication date: 20200612 |