CN111264716A

CN111264716A - Pet health care composition

Info

Publication number: CN111264716A
Application number: CN201911294440.1A
Authority: CN
Inventors: 包伟平
Original assignee: 包伟平
Priority date: 2019-12-16
Filing date: 2019-12-16
Publication date: 2020-06-12

Abstract

The invention discloses a pet health care composition in the technical field of pet nutrition, which comprises the following components in percentage by weight: 1-30% of carbon particles, 0.5-5% of green mussel, 0.5-5% of beer yeast powder, 0.5-5% of digestive ferment, 0.5-5% of inulin and the balance of auxiliary materials; the invention aims to provide a pet health-care composition which can protect kidney cells, protect the metabolism of the urinary system, avoid the damage of harmful substances, promote the rapid repair and recovery of kidney tissues by hormones and maintain the kidney function.

Description

Pet health care composition

Technical Field

The invention relates to the technical field of pet nutrition, and particularly relates to a pet health-care composition.

Background

With the rapid development of national economy in China, the living standard of people is continuously improved, high-quality life becomes a target pursued by people, more and more attention is paid to dog and cat, dog and cat feeding becomes fashionable, the breeding types of dog and cat in China are increased, but the probability of disease occurrence of dog and cat is increased.

The kidney failure of the pet is a common disease of the pet at present, the disease is not easy to be found in the early stage, but when people start to find the disease, the pet often has severe failure, and the disease cannot be cured, so that the disease is referred to as the pet kidney disease, but the disease cannot be prevented like canine plague or some infectious diseases at present, and the invention improves the defect.

Disclosure of Invention

In order to overcome the defects of the background art, the invention aims to provide a pet health-care composition which can protect kidney cells, protect the metabolism of the urinary system, avoid the damage of harmful substances, promote the rapid repair and recovery of kidney tissues by hormones and maintain the kidney function.

The technical scheme adopted by the invention is as follows:

a pet health composition comprises the following components in percentage by weight: 1-30% of carbon particles, 0.5-5% of green mussel, 0.5-5% of beer yeast powder, 0.5-5% of digestive ferment, 0.5-5% of inulin and the balance of auxiliary materials.

Preferably, the composition comprises the following components in percentage by weight: 10-20% of carbon particles, 1-3% of green mussels, 1-3% of beer yeast powder, 1-3% of digestive ferment, 1-3% of inulin and the balance of auxiliary materials.

Furthermore, the carbon particles are polyacrylonitrile activated carbon fibers, the average diameter of the polyacrylonitrile activated carbon fibers is 5-30 micrometers, the BET specific surface area is greater than or equal to 390 square meters per gram, the total acidic groups are greater than 1.2meq/g or the total basic groups are greater than 1 meq/g.

Further, the polyacrylonitrile active carbon fiber is prepared by the following operation steps: the paint comprises the following components in percentage by weight: 85-90% polyacrylonitrile plus 10-15% rayon or plus 10-15% lime, is treated in aqueous carbon dioxide gas at 700-1000 ℃ for 1-60 minutes.

Preferably, the polyacrylonitrile has an average diameter of 5 to 10um and an average length of 10 to 20 um.

Furthermore, active particles are attached to the surface of the polyacrylonitrile active carbon fiber.

Preferably, the active particles are one or more of silver, gold, aluminum, zinc, copper and titanium dioxide.

Preferably, the particle size of the active particles is 1nm to 500 um.

Further, the auxiliary materials comprise one or more of a binder, a disintegrant, a lubricant, a flavoring agent and a diluent;

the adhesive is one or more of starch, maltodextrin, sodium alginate and polyvinylpyrrolidone;

the disintegrating agent is one or more of sodium carboxymethyl starch, sodium carboxymethyl cellulose and microcrystalline cellulose;

the lubricant is one or more of silicon dioxide, magnesium stearate, sodium stearate and talcum powder;

the flavoring agent is one or more of chicken liver powder, pork liver powder and beef powder;

the diluent is one or more of lactose, glucose and mannitol.

Further, the auxiliary materials comprise the following components in percentage by weight relative to the total weight of the pet health composition: 1 to 15 percent of adhesive, 0.5 to 10 percent of disintegrating agent, 0.5 to 10 percent of lubricant, 0.01 to 1 percent of flavoring agent and the balance of diluent.

The invention has the beneficial effects that:

1. the invention mixes the carbonaceous particles, the green mussels, the beer yeast powder, the digestive ferment and the inulin together to prepare the medical health care composition for pets, which can protect kidney cells, protect the metabolism of the urinary system, avoid the damage of harmful substances, promote the rapid repair and recovery of kidney tissues and maintain the functions of the kidney by hormone, and the carbon material can also promote the absorption of the digestive tract and treat the inflammation of the liver;

2. the invention can be used together with common food to achieve daily maintenance, and because of the respective efficacies of the green mussel, the beer yeast powder, the digestive ferment and the inulin, the invention can improve the kidney function, the digestive system and the liver function of the pet, improve the immunity of the pet and improve the activity of the pet.

Drawings

FIG. 1 is a graph showing the change in the value of Creatine (CREA) fed to experimental mice for five weeks;

FIG. 2 is a graph showing the change of GTP value of experimental mice fed for five weeks;

FIG. 3 is WT-ctrl, 200 ×: normal glomerular (arrow) and tubular (arrow) patterns;

FIG. 4 is Nep-ctrl, 200 ×: tubular degeneration, atrophy and regeneration (arrow);

FIG. 5 is Nep-ctrl, 200 ×: tubular dilation, endothelial cells shed in the lumen (arrows);

FIG. 6 is Nep-ctrl, 100 ×: glomerular enlargement (arrow), interstitial nephritis and fibrosis;

FIG. 7 shows Nep-Spring, 100 ×: tubular dilation, hyaline cylinders (asterisks), glomerular atrophy (arrowheads), renal interstitial fibrosis and renal interstitial inflammation (three corners);

FIG. 8 shows Nep-Spring, 100 ×: tubular degeneration and atrophy, renal adventitial fibrosis and hepatocyte attachment (arrow);

FIG. 9 is Nep-Spring, 200 ×: hyaline-like lesions of the silk spheroid (arrow) and infiltration of inflammatory cells;

fig. 10 is a Nep-MGC, 200 ×: spheroid contraction, tubular dilation and renal interstitial fibrosis;

FIG. 11 shows WT-nor, 200 ×: normal silk spheroid and renal tubule morphology;

fig. 12 is Nep-MGC 1, 100 ×: tubular dilation, endothelial cells shedding into the lumen;

FIG. 13 is Nep-MGC 1, 100 ×: tubular degeneration and shrinkage, interstitial inflammation of the kidney (arrow);

FIG. 14 is Nep-MGC 1, 200 ×: tubulointerstic fibrosis, interstitial fibrosis of the kidney (arrow);

FIG. 15 shows WT-ctrl, PAS, 200 ×: normal silk spheroid and renal tubule morphology;

FIG. 16 is WT-ctrl, PAS, 200 ×: hyaline-like lesions of the silk spheroid are accompanied by thickening of the basement membrane (arrow);

FIG. 17 shows Nep-Spring, PAS, 100 ×: corresponding to fig. 9, the silk spheroid hyaline-like lesion was accompanied by thickening of the basement membrane;

FIG. 18 is Nep-MGC, PAS, 200 ×: renal tubule pathology-thickening of basement membrane (at arrows);

FIG. 19 shows WT-nor, PAS, 100 ×: normal silk spheroid and renal tubule morphology;

FIG. 20 is Nep-MGC 1, PAS, 200 ×: thickening of the basement membrane (left half);

FIG. 21 shows WT-ctrl, TRI, 200 ×: normal distal tubule morphology at the renal pelvis;

FIG. 22 shows Nep-ctrl, TRI, 200 ×: renal interstitial fibrosis at the renal pelvis (arrow);

FIG. 23 shows Nep-Spring, TRI, 200 ×: in comparison to fig. 7, renal interstitial fibrosis at the renal cortex;

FIG. 24 shows Nep-MGC, TRI, 200 ×: adventitia and renal interstitial fibrosis;

FIG. 25 shows WT-nor, TRI, 200 ×: normal filament spheroid and tubular morphology at the cortex;

FIG. 26 is Nep-MGC 1, TRI, 200 ×: renal interstitial fibrosis at the cortex.

Detailed Description

The following further describes embodiments of the present invention with reference to the drawings. It should be noted that the description of the embodiments is provided to help understanding of the present invention, but the present invention is not limited thereto.

Example a 1-6: preparation of polyacrylonitrile active carbon fiber

Example A1

The paint comprises the following components in percentage by weight: 85% of polyacrylonitrile having an average diameter of 5um and an average length of 10um plus 15% of rayon thread, treated in aqueous carbon dioxide gas at 700 ℃ for 1 minute; the prepared polyacrylonitrile active carbon fiber has the average diameter of 5um, the BET specific surface area of more than or equal to 390 square meters per gram, the total acidic groups of more than 1.2meq/g or the total basic groups of more than 1 meq/g; silver with the grain diameter of 1-100 nm is attached to the surface of the polyacrylonitrile active carbon fiber to be used as active particles; the polyacrylonitrile-based activated carbon fiber is ready for use as carbonaceous fine particles.

Example A2

The paint comprises the following components in percentage by weight: 88% of polyacrylonitrile having an average diameter of 8um and an average length of 15um plus 12% of rayon thread, treated in aqueous carbon dioxide gas at 800 ℃ for 30 minutes; the prepared polyacrylonitrile active carbon fiber has the average diameter of 15um, the BET specific surface area of more than or equal to 400 square meters per gram, the total acidic groups of more than 1.3meq/g or the total basic groups of more than 1.1 meq/g; gold with the grain diameter of 1-10 um is attached to the surface of the polyacrylonitrile active carbon fiber to be used as active particles; the polyacrylonitrile-based activated carbon fiber is ready for use as carbonaceous fine particles.

Example A3

The paint comprises the following components in percentage by weight: 90% of polyacrylonitrile having an average diameter of 10um and an average length of 20um plus 10% of rayon thread, treated in aqueous carbon dioxide gas at 1000 ℃ for 60 minutes; the prepared polyacrylonitrile active carbon fiber has the average diameter of 30um, the BET specific surface area of more than or equal to 420 square meters per gram, the total acidic groups of more than 1.5meq/g or the total basic groups of more than 1.3 meq/g; aluminum with the grain diameter of 490-500 um is attached to the surface of the polyacrylonitrile active carbon fiber as active particles; the polyacrylonitrile-based activated carbon fiber is ready for use as carbonaceous fine particles.

Example A4

Rayon in example a1 was replaced with pitch and silver was replaced with zinc, all other conditions and steps being the same as in example a 1.

Example A5

Rayon in example a2 was replaced with pitch and gold was replaced with copper, with the other conditions and steps being the same as in example a 2.

Example A6

Rayon in example A3 was replaced with pitch and aluminum was replaced with titanium dioxide, all other conditions and procedures being the same as in example A3.

Example B1-6: preparation of pet health composition

Example B1:

accurately weighing the following components in percentage by weight: polyacrylonitrile activated carbon fiber prepared in example a1 as carbonaceous particulates 1%, green mussel 0.5%, beer yeast powder 0.5%, digestive ferment 0.5%, inulin 0.5%, 1% starch as binder, 0.5% sodium carboxymethyl starch as disintegrant, 0.5% silicon dioxide as lubricant, 0.01% chicken liver powder as flavoring agent, and the balance of lactose as diluent to make up 100%; mixing completely to obtain the final product.

Example B2:

accurately weighing the following components in percentage by weight: polyacrylonitrile activated carbon fiber prepared in example a2 as carbonaceous fine particles 5%, green mussel 4%, brewer's yeast powder 4%, digestive ferment 4%, inulin 4%, maltodextrin 3% as binder, sodium carboxymethylcellulose 3% as disintegrant, magnesium stearate 2% as lubricant, pork liver powder 0.1% as flavor, and the balance glucose as diluent to make up 100%; mixing completely to obtain the final product.

Example B3:

accurately weighing the following components in percentage by weight: polyacrylonitrile activated carbon fiber prepared in example a3 as carbonaceous particulate 30%, green mussel 5%, brewer's yeast powder 5%, digestive ferment 5%, inulin 5%, 6% sodium alginate as binder, 6% microcrystalline cellulose as disintegrant, 4% sodium stearate as lubricant, 0.3% beef powder as flavor, the balance being mannitol to make up 100%; mixing completely to obtain the final product.

Example B4:

accurately weighing the following components in percentage by weight: polyacrylonitrile activated carbon fiber prepared in example a4 as carbonaceous fine particles 10%, green mussel 1%, brewer's yeast powder 1%, digestive ferment 1%, inulin 1%, 9% polyvinylpyrrolidone as binder, 8% microcrystalline cellulose as disintegrant, 6% talc as lubricant, 0.5% chicken liver powder as flavoring agent, and the balance lactose as diluent to make up 100%; mixing completely to obtain the final product.

Example B5:

accurately weighing the following components in percentage by weight: polyacrylonitrile activated carbon fiber prepared in example a5 as carbonaceous fine particles 15%, green mussel 1.5%, beer yeast powder 1.5%, digestive ferment 1.5%, inulin 1.5%, polyvinylpyrrolidone 12% as binder, sodium carboxymethyl starch 9% as disintegrant, sodium stearate 8% as lubricant, pork liver powder 0.8% as flavoring agent, and the balance being glucose to make up 100%; mixing completely to obtain the final product.

Example B6:

accurately weighing the following components in percentage by weight: polyacrylonitrile activated carbon fiber prepared in example a6 as carbonaceous particulates 20%, green mussel 3%, brewer's yeast powder 3%, digestive ferment 3%, inulin 3%, sodium alginate 15% as binder, microcrystalline cellulose 10% as disintegrant, talc 10% as lubricant, beef powder 1% as flavoring agent, and the balance lactose as diluent to make up 100%; mixing completely to obtain the final product.

The finished granules prepared in example B1-6 were selected to detect clostridium perfringens, pathogenic escherichia coli, listeria monocytogenes, and salmonella according to the bulletin-corrected food microorganism detection method, with the following detection results:

the invention is further illustrated by the following test data, and the effect of oral administration of the invention on the liver and kidney function index in the blood of a kidney-cutting rat is provided.

The detection method comprises the following steps: 3 male rats were selected, divided into normal, control and experimental rats: feeding normal feed to normal rats; control mice 5/6 were renectomized (one full resection, the other 2/3 resection) and fed normal diet; the experimental mice 5/6 were fed with the feed containing 5% of the present invention every day after nephrectomy (one complete resection and 2/3 resection), were freely fed to observe body weight and blood, and were subjected to clinical biochemical analysis after serum separation for 5 weeks.

Glutamate-pyruvate transaminase (GTP), an enzyme involved in amino acid metabolism, is present in hepatocytes. A higher GTP than normal indicates that the liver may be experiencing problems.

Creatinine (CREA) is a metabolic product derived from the muscle activity of the body and is an important factor causing glomerulosclerosis, and if the concentration of CREA in the blood is high, renal dysfunction is reflected, and the worse the renal function is, the higher the concentration in the blood is.

As shown in fig. 1, the CREA concentration of the nephrectomized 5/6 control mice was significantly higher in five weeks, and in the process, the CREA value of the mice fed the present invention daily in the first week was significantly decreased, which proved that the CREA value could be decreased by eating the composition, and the composition had a better therapeutic effect on kidney-related diseases.

As shown in figure 2, the control mice with nephrectomy 5/6 have obviously higher GTP value and abnormal liver function, while the experimental mice fed with the traditional Chinese medicine composition have obviously reduced GTP value which is basically equal to the index of normal mice, thereby proving that the composition can reduce the GTP value when being eaten and has better curative effect on abnormal liver function.

To further demonstrate the efficacy of the present invention, kidney pathological diagnosis was performed by formalin-fixing the 3 SD rat kidney samples. The results for the samples are as follows:

method for diagnosing tissue pathology

1. Control group was divided into two sections (one on each side) and treatment group (kidney-excised mice) was divided into one section

2. Samples were sent for paraffin embedding with a slice thickness of 3 μm and analyzed by hematoxin and eosin (H & E), Periodic Acid Schiff (PAS) and Masson's Trichrome (TRI) staining.

3. The lesion size grading for pathological diagnosis is classified into 5 grades according to the method described by Shackelford et al in clinical Pathology, Vol 30, No1, pp93-96,2002: 1-minimal (< 1); 2-slide slight (1-25); moderate (26-50) 3 ═ moderate; 4- (modetely segment) moderately severe (51-75); severe/high (76-100) was severe.

Histopathological diagnosis results:

the pathological conditions observed by microscope are described in "histopathological incidence table". The sections are shown in FIGS. 3-14, and the special stains (PAS and TRIstatin) are shown in FIGS. 15-26. The change in renal tissue type and the degree of damage are organized as follows:

chronic kidney disease:

different degrees of kidney damage were observed except for control mice, and the diagnostic criteria were as follows:

tubular associated lesions: in addition to control mice, tubular dilation, tubular degeneration/regeneration with multiple tubular alkalinization, nuclear crowding and basement membrane thickening were observed with mild to moderate damage. Tubular dilation is often accompanied by tubular necrosis/degeneration and tubular clearing, resulting in blood flow stasis, severe hemodynamic changes, and electrolyte or water loss. Transparent columns (protein gel deposits), cell debris, and light to moderate endothelial cells (normal or flat) were also observed in the lumen. Tubular degeneration refers to reversible changes or irreversible early necrosis.

Silk spheroid associated lesions: including enlargement or atrophy of the silk spheroid, accompanied by dilatation of the bursa of Fabricius and hyaline-like lesions of the silk spheroid. The above pathological conditions were observed with little to mild lesions, except in the control group. Such damage is induced by changes in fluid pressure within the silk spheroids, causing the silk spheroids and the tubules connected thereto to degenerate. Chronic silk spheroid lesions will eventually lead to atrophy. The accumulation of immunoglobulin during the degeneration of silk spheres causes clear-like lesions of silk spheres, which can be confirmed by PAS staining.

Renal interstitial fibrosis: similarly, with the exception of the control group, renal interstitial fibrosis was observed, with mild to moderate lesions. Such damage is effected by adjacent damaged tubules and may also be associated with other renal tubular pathologies.

In addition to this, other chronic kidney pathologies such as adventitial fibrosis and renal interstitial inflammation were also observed in kidney-resected mice. Adventitial fibrosis is accompanied by hepatocyte adhesion (possibly caused by nephrectomy). The degree of renal interstitial inflammation was mild to moderate. Multifocal inflammatory cell infiltration in the interstitium is dominated by mononuclear spheres, with the degree and severity of infiltration being directly proportional to age, and associated with tubular or filament lesions.

Histopathological incidence table:

x: no obvious damage;

a: merge H & E and PAS staining results b: combining the H & E and TRI staining results;

grading standard of damage degree: 1 ═ minor (< 1); 2-slot (1-25); 3-moderate (26-50); 4- (modetheysevere (51-75); 5 ═ severe/high (76-100);

and (4) conclusion:

except for the control group, the chronic renal disease trait was observed. In group 1, severe compensatory lesions such as tubular and silk spheroid changes, renal interstitial inflammation and fibrosis were observed in kidney-deprived mice (Nep-ctrl) not fed with the present invention. After feeding Spring and autumn bamboo charcoal powder (Nep-Spring), the damage degree of renal tubules and silk balls is observed to be more serious; whereas feeding the present invention (Nep-MGC) was less severe than Nep-ctrl. In group 2, partial renal tissue lesions were observed when fed with the present invention (Nep-MGC 1) compared to the control group (WT-nor), but were milder compared to kidney-excised mice (Nep-ctrl).

In conclusion, the invention has better curative effect on impaired renal function and abnormal liver function, but simultaneously has great function on anti-inflammatory action, especially on inflammatory diseases such as arthritis, arthralgia and the like because of having green and pleasant shellfish components, and can strengthen bones; the beer yeast powder contains rich vitamin B group, multiple vitamins and minerals, more than 50 percent of protein and complete amino acid group, and is the best source for supplementing high-quality protein. The beer yeast powder also contains rich dietary fiber, which is helpful for relieving constipation; digestive ferment is a substance with special biological activity and composed of amino acid, exists in all living animals and plants, and is a substance for maintaining the life activities of normal functions of organisms, digesting food, repairing tissues and the like; the inulin is a natural polysaccharide body, is made from a plurality of plants, is considered to be a soluble fiber, is classified in prebiotics, is helpful for intestines and stomach of pets, and can be taken as a health care device for pets for a long time through a combined preparation of the components, so that the inulin has better medical effect, has the effects of inhibiting glutamic-pyruvic transaminase (GTP) and Creatinine (CREA), preventing the concentration of in vivo ammonia-pyruvic transaminase (GTP) and Creatinine (CREA) from being increased, improving the kidney function and the liver function of the pets, improving the digestive system of the pets and enhancing the immunity and the activity of the pets.

Therefore, the present invention can be used as a medicine for treating the kidney diseases of pets, is used for treating pets suffering from diseases, can also be used for daily maintenance and eating of pets, and is a functional health-care composition for preventing pet diseases. It will be apparent to those skilled in the art that various changes, modifications, substitutions and alterations can be made in the embodiments without departing from the principles and spirit of the invention, and these embodiments are within the scope of the invention.

Claims

1. A pet health composition characterized by: comprises the following components in percentage by weight: 1-30% of carbon particles, 0.5-5% of green mussel, 0.5-5% of beer yeast powder, 0.5-5% of digestive ferment, 0.5-5% of inulin and the balance of auxiliary materials.

2. A pet care composition according to claim 1, wherein: comprises the following components in percentage by weight: 10-20% of carbon particles, 1-3% of green mussels, 1-3% of beer yeast powder, 1-3% of digestive ferment, 1-3% of inulin and the balance of auxiliary materials.

3. A pet care composition according to claim 1 or 2, wherein: the carbon particles are polyacrylonitrile active carbon fibers, the average diameter of the polyacrylonitrile active carbon fibers is 5-30 um, the BET specific surface area is larger than or equal to 390 square meters per gram, the total acidic group is larger than 1.2meq/g or the total basic group is larger than 1 meq/g.

4. A pet care composition according to claim 3, wherein: the polyacrylonitrile active carbon fiber is prepared by the following steps: the paint comprises the following components in percentage by weight: 85-90% polyacrylonitrile plus 10-15% rayon or plus 10-15% lime, is treated in aqueous carbon dioxide gas at 700-1000 ℃ for 1-60 minutes.

5. The pet health composition of claim 4, wherein: the average diameter of polyacrylonitrile is 5-10 um, and the average length is 10-20 um.

6. The pet health composition of claim 4, wherein: active particles are attached to the surface of the polyacrylonitrile active carbon fiber.

7. The pet care composition of claim 6, wherein: the active particles are one or more of silver, gold, aluminum, zinc, copper and titanium dioxide.

8. The pet care composition of claim 6, wherein: the particle size of the active particles is 1 nm-500 um.

9. A pet care composition according to claim 3, wherein: the auxiliary materials comprise one or more of a bonding agent, a disintegrating agent, a lubricating agent, a flavoring agent and a diluting agent;

the diluent is one or more of lactose, glucose and mannitol.

10. A pet care composition according to claim 9, wherein: the auxiliary materials comprise the following components in percentage by weight relative to the total weight of the pet health care composition: 1 to 15 percent of adhesive, 0.5 to 10 percent of disintegrating agent, 0.5 to 10 percent of lubricant, 0.01 to 1 percent of flavoring agent and the balance of diluent.