CN111264430A

CN111264430A - Method for breeding rabdosia lophanthide in laboratory

Info

Publication number: CN111264430A
Application number: CN202010140286.9A
Authority: CN
Inventors: 角建林; 郑红; 王文林; 李波
Original assignee: Kunming Medical University
Current assignee: Kunming Medical University
Priority date: 2020-03-03
Filing date: 2020-03-03
Publication date: 2020-06-12

Abstract

The invention relates to a method for breeding creek crabs in a laboratory, wherein the bred crab seeds are 30-50g in weight and are healthy and complete; sterilizing and cleaning with potassium permanganate solution, and placing into a breeding box, wherein the number of the fed 3 male crabs is 6-9 female crabs; the stream crab breeding box is provided with a water pool with the water depth of 5cm, is connected with the aquarium filtering system, keeps water circulation, is provided with a wood board higher than the water pool for the stream crab to live and inhabit, and is placed in the wooden nest; the room temperature is 21-26 ℃, the illumination is carried out for 12 hours, and the illumination intensity is 80-120 Lx; the brook crabs put into the breeding boxes are cultured for about 15 days, the female crabs with the abdominal limbs attached with eggs of the female crabs are gathered into another breeding box to hatch the larvae, and the reproductive capacity of the female crabs and the survival rate of the young crabs are fully ensured. The artificial efficient propagation of the river crabs for experiments under laboratory conditions is realized.

Description

Method for breeding rabdosia lophanthide in laboratory

Technical Field

The invention relates to a wild crab breeding method under laboratory conditions.

Background

The stream crab is about 30g of fresh water crab, the majority of the holes are in the holes on the sides of the stream, river, lake and ditch, and the arthropod of the river crab inhabits on half land. As the individual is too small and has no value of artificial large-scale breeding, breeding and eating, scientific researchers cannot purchase the stream crabs needed for experimental research from the market. At present, few reports of related technologies for breeding the river crabs manually exist, and the river crabs are still in a blank state. The group of the projects applies the creek crabs for long-term research and research of the diseases of the fasciola gigantea and development of animal models of hepatic fibrosis and pulmonary fibrosis, and in order to meet the research requirements, the research on feeding and breeding methods under the condition of a creek crab laboratory is always carried out for many years.

Disclosure of Invention

The invention aims to provide a method for breeding rabbitis in a laboratory.

The technical scheme of the invention is as follows: a method for breeding rabdosia lophanthide in a laboratory comprises the following steps:

1. selection of breeding crabs for laboratory breeding: capturing adult Yunnan river crab in the wild with the weight of 30-50g and the body being healthy and complete;

2. soaking and disinfecting the crab seeds for 15 minutes by using two-thousandth of potassium permanganate aqueous solution, and then soaking the crab seeds for 15 minutes by using clear water, and putting the crab seeds into a brook crab breeding box;

3. putting 3 male crabs and 6-9 female crabs into the stream crab breeding box, wherein the male-female ratio is 1: 2-3;

4. one half of the brook crab breeding box is a water tank, the water depth is kept at 5cm, the water tank is connected with a conventional aquarium filtering system, and the water in the water tank is kept to circulate uninterruptedly;

5. one half of the brook crab breeding box is a wood board for the brook crab to live and inhabit, the wood board is 0.5-1cm higher than the water surface of the pool, and 4 wooden nests with openings at two ends are placed;

6. the method is characterized in that a wire mesh for preventing other animals from entering a brook crab breeding room is required to be installed, the room is kept ventilated, the temperature is controlled at 21-26 ℃, the automatic timing control is carried out within 12 hours, and the control intensity is 80-120Lx (lux);

7. about 15 days after the stream crabs are put into the breeding boxes, whether eggs are attached to the abdominal-limb setae of the female crabs is checked, and the female crabs with the attached eggs are timely gathered into another breeding box to hatch the larvae, so that the reproductive capacity of the female crabs and the survival rate of the young crabs are fully ensured;

8. the crab breeding box is 100 cm long, 60 cm wide and 30cm high, and is made of stainless steel plates with the thickness of 1 mm;

9. the inner space of the rectangular nest is 10 cm in length, 6 cm in width and 3cm in height, and the wood board is used and has no pungent smell, and the thickness of the wood board is 1 cm.

The invention can reach the conditions of comprehensive nutrition of the feed, superior temperature and illumination and the like compared with natural conditions under laboratory conditions, and fully ensures the reproductive capacity of female crabs and the survival rate of young crabs. Can be needed for experiments

To provide a crab stream with reliable quality.

Detailed Description

It will be appreciated by those skilled in the art that the following examples are illustrative of the invention only and should not be taken as limiting the scope of the invention. The examples do not specify particular techniques or conditions, and are performed according to the techniques or conditions described in the literature in the art or according to the product specifications. The reagents or instruments are not indicated by manufacturers, and are conventional products available by purchase

Example 1

1. Selecting the breeding crabs: capturing adult Yunnan river crab in the wild with the weight of 30-50g and the body being healthy and complete;

2. soaking and disinfecting the crab seeds for 15 minutes by using two-thousandth of potassium permanganate aqueous solution, and then soaking the crab seeds for 15 minutes by using clear water, and putting the crab seeds into a brook crab breeding box; the breeding box is made of 1mm stainless steel plates, and each breeding box is 100 cm long, 60 cm wide and 30cm high;

3. putting 3 male crabs and 6 female crabs into a breeding box, wherein the male-female ratio is 1: 2;

4. one half of the brook crab breeding box is a water tank, the water depth is kept about 5cm, the water tank can be connected with a conventional aquarium filtering system, and the water in the water tank is kept to circulate uninterruptedly;

5. one half of the brook crab breeding box is a wood board (0.5-1 cm higher than the water surface of the pool) for the brook crab to live and rest, and 4 wooden nests with openings at two ends are placed, and the internal space of each rectangular nest is 10 cm in length, 6 cm in width and 3cm in height;

6. installing a wire mesh for preventing other animals from entering a brook crab breeding room, keeping ventilation in the room, controlling the temperature at 21-26 ℃, automatically illuminating for 12 hours at regular time, and controlling the illumination intensity at 80-120Lx (lux);

about 7.15 days, whether eggs are attached to the abdominal-limb bristles of the female crabs is checked, the female crabs with the attached eggs are timely gathered into another breeding box to hatch the larvae, and the reproductive capacity of the female crabs and the survival rate of the young crabs are fully ensured.

Example 2

All the steps are the same as example 1, except that 3, 3 male crabs and 9 female crabs are put into the stream crab breeding box in the step, and the male-female ratio is 1: 3.

The foregoing shows and describes the general principles, essential features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims

1. A method for breeding creek crabs in a laboratory is characterized in that: the method comprises the following steps:

1) selecting breeding crabs for laboratory breeding: capturing adult Yunnan river crab in the wild with the weight of 30-50g and the body being healthy and complete;

2) soaking and disinfecting the crab seeds for 15 minutes by using two-thousandth of potassium permanganate aqueous solution, and then soaking the crab seeds for 15 minutes by using clear water, and putting the crab seeds into a brook crab breeding box;

3) 3 male crabs and 6-9 female crabs are put into the stream crab breeding box, and the male-female ratio is 1: 2-3;

4) one half of the brook crab breeding box is a water tank, the water depth is kept at 5cm, the water tank is connected with an aquarium filtering system, and the water in the water tank is kept to circulate uninterruptedly;

5) one half of the brook crab breeding box is a wood board for the brook crab to live and inhabit, the wood board is 0.5-1cm higher than the water surface of the pool, and 4 wooden nests with openings at two ends are placed;

6) installing a wire mesh for preventing other animals from entering a brook crab breeding room, keeping ventilation in the room, controlling the temperature at 21-26 ℃, automatically illuminating for 12 hours at regular time, and controlling the illumination intensity at 80-120 Lx;

7) and (4) checking whether eggs are attached to the bristles of the abdominal limbs of the female crabs after 15 days, and timely collecting the female crabs with the attached eggs into another breeding box to hatch the larvae, thereby fully ensuring the reproductive capacity of the female crabs and the survival rate of the young crabs.

2. The method for breeding rabdosia lophanthide in laboratory according to claim 1, wherein: the crab breeding box is 100 cm long, 60 cm wide and 30cm high.

3. The method for breeding rabdosia lophanthide in laboratory according to claim 1, wherein: the inner space of the rectangular nest is 10 cm long, 6 cm wide and 3cm high.