CN111256775B - Assimilation box system for rapidly determining effective volume and using method - Google Patents
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- CN111256775B CN111256775B CN202010116604.8A CN202010116604A CN111256775B CN 111256775 B CN111256775 B CN 111256775B CN 202010116604 A CN202010116604 A CN 202010116604A CN 111256775 B CN111256775 B CN 111256775B
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Abstract
The invention discloses a rapid speedAssimilation box system for determining effective volume and use method thereof, wherein the assimilation box system comprises assimilation box and CO2Analyzer, assimilation tank and CO2Passing CO between analyzers2Air inlet line and CO2The gas outlet pipeline is communicated, a sensor module is fixedly arranged in the assimilation box, and a sensor of the sensor module comprises an air pressure sensor, a temperature and humidity sensor and a light intensity sensor; the sensor module is connected with the data acquisition/display module through a small hole sealed on the side surface of the assimilation box, and the assimilation box is also provided with a pressure balancing device and CO2A gas injection device; CO 22The injection device comprises a plate penetrating joint and a gas transmission device, and a sealing gasket is fixedly arranged in the plate penetrating joint; pressure balance device includes atmospheric pressure balancing tubule, atmospheric pressure balancing tubule one end set firmly in assimilation case lateral wall, with assimilation incasement portion intercommunication, balloon or buffer flask are connected to one end in addition. The invention can quickly and accurately determine the effective action volume in the system and eliminate the interference of irregular shape of the measured object on the premise of keeping the normal physiological and ecological processes of the measured object.
Description
Technical Field
The invention relates to a gas flux measuring system, in particular to an assimilation box system capable of rapidly determining effective volume and a using method.
Background
In related researches of zoology, botany and ecology, gas flux of carbon dioxide, methane, water vapor and the like is a basic measurement index, and measurement of the indexes has important significance for researching aspects such as energy metabolism and substance circulation of organisms (animals, plants, microorganisms and the like). The most common method for measuring gas flux at present is a closed-circuit type assimilation box method, gas concentration in the assimilation box is measured through a corresponding gas analyzer, and the gas flux of a measured object is calculated according to a change value of the gas concentration within a certain time. Assimilation box extensively is used for indoor control experiment and open-air normal position measurement experiment: such as insect, avian, and mammalian respiration rate measurements, potted plant photosynthetic/respiratory/transpiration measurements, soil microbial gas flux measurements, and field community greenhouse gas flux in situ measurements.
In the process of applying the assimilation box method by scientific researchers, the assimilation box can be improved or expanded according to special requirements of scientific researches, so that automatic control or multi-path control is achieved, the using efficiency of an instrument is improved, meanwhile, the labor cost is saved, or the assimilation box can adapt to special purpose requirements in specific scientific researches or teaching works. Although the assimilation box method has been widely used by researchers and has achieved a great deal of success, the following problems are still prevalent in the current application of this method: 1. in the use process of the assimilation box, scientific researchers often ignore the volume of a measured object (an animal, a plant, a soil sample, a container thereof and the like), and the actual action volume of the assimilation box system is overestimated after the measured object is placed in the assimilation box system, so that the gas flux measured value is higher than the actual value, and the overestimation depends on the volume ratio of the measured object to the assimilation box system. 2. The actual volume of the assimilation box system is composed of a plurality of components, and accurate calculation of the volume of the assimilation box is difficult or consumes a great deal of time or energy, so that the efficiency of scientific research work and the reliability of measurement results are reduced.
The actual active volume of the assimilation box system, the conventional method, is generally accurately calculated as follows: v is Va+Vc+Vb-VmIn the formula, V: the actual volume of the assimilation tank system; va: an analyzer module volume; vc: the volume of the pipeline; vb: assimilating the box body volume of the box; vm: the volume occupied by the subject. Albeit VbAnd VcCalculation of measurements before measurement, but VaAnd VmIs rather difficult to calculate accurately. For example, the analyzer module needs to be driven by an air pump, and positive pressure or negative pressure exists in front and back pipelines of the air pump and air in the analyzer and is inconsistent with the air pressure of the assimilation box, so that V is formedaDifficult to determine accurately; the shape of the object to be measured is often irregular, and its volume (V) is measured and calculatedm) Time and labor are wasted, and the gas flux of the measured object is easily influenced.
In summary, in the related fields of biology and ecology, there is a need to develop or improve an assimilation box system and method for determining the actual volume rapidly and accurately so as to improve the working efficiency of scientific research and the reliability of the measurement result.
Disclosure of Invention
To solve the problemThe present invention is based on the principle of Moore's law (amount of homothermal homologies) with CO2Flux measurements are an example, and a system and method of use are provided for quickly and accurately determining the volume of an assimilation box system. The invention is realized by the following technical scheme.
An assimilation box system for rapidly determining effective volume comprises an assimilation box and CO2An analyzer, the assimilation tank and CO2Passing CO between analyzers2Air inlet line and CO2The gas outlet pipeline is communicated, a sensor module is fixedly arranged in the assimilation box, and a sensor of the sensor module comprises an air pressure sensor, a temperature and humidity sensor and a light intensity sensor; the sensor module is connected with the data acquisition/display module through a small hole sealed on the side surface of the assimilation box, and the assimilation box is also provided with a pressure balancing device and CO2A gas injection device; the CO is2The gas injection device comprises a plate penetrating joint and a gas transmission device, and a sealing gasket is fixedly arranged in the plate penetrating joint; pressure balance device includes atmospheric pressure balancing tubule, atmospheric pressure balancing tubule one end sets firmly assimilation case lateral wall, with the inside intercommunication of assimilation case, one end is connected with balloon or cushion flask in addition.
Preferably, the assimilation box is a sealed cylindrical or square box body, a top-opening, side-opening or bottom-opening sealing cover is arranged on the assimilation box, and the sealing cover and the box body are sealed through a sealing strip, a sealing glue or a water tank.
Preferably, the assimilation box top plate is provided with two quick connectors which are respectively connected with the CO2Air inlet line and CO2The air outlet pipeline is connected.
Preferably, the gas delivery device is an injection syringe, and the injection needle passes through a sealing gasket in the plate penetrating joint through a thin needle head to complete the quantitative volume of CO2And (5) injecting.
Preferably, the plate penetrating joint is connected with an extension pipe at a short opening in the assimilation box, and the extension pipe extends to the bottom of the assimilation box.
Preferably, the assimilation box inner wall installation mixing fan of wearing the board joint adjacent position guarantees the quick mixing of the gas in the assimilation box.
Preference is given toOf said CO2The analyzer is used for detecting CO by a non-dispersive infrared absorption method2Concentration, a built-in circulating pump or an external serial circulating pump, and the frequency of sampling record is not lower than 1 Hz.
A method of using an assimilation box system including the above rapid determination of effective volume, comprising the steps of:
a) after all the devices are electrified and preheated, putting the object to be tested into the assimilation box, and sealing the assimilation box;
b) to be treated with CO2After the change slope is stable, starting to record data of the analyzer and the sensor;
c) data were recorded until the slope was stable by CO2Gas injection device for accurately injecting pure CO of known volume2；
d) Continue recording data to CO2The change slope tends to be stable;
e) according to the recorded data, the actual volume of the assimilation box system is calculated in real time by a microcomputer or accurately by manual processing, and the CO of the measured object is further calculated2Flux.
More preferably, CO is injected2Assimilation of CO in the front2Concentration of CO close to ambient2Concentration, CO after injection2The concentration increment is between 50 and 500 ppm.
More preferably, CO is injected2Pre CO2Concentration versus time was fitted: y ═ ax + b1(ii) a CO injection2And fitting the data after the slope is stable by using the slope before injection: y ═ ax + b2；CO2The increment of the concentration is (b)2-b1)。
The invention has the beneficial effects that:
the assimilation box system and the use method for quickly determining the effective volume can quickly and accurately determine the effective acting volume in a system on the premise of keeping the normal physiological and ecological processes of a measured object, eliminate interference caused by the irregular shape of the measured object and ensure that a further gas flux calculation result is more scientific and reliable. The invention aims to measure CO2Flux processes (e.g. net photosynthesis in plants, animals and plants andnet respiration of the soil, etc.) or may be used independently, i.e. only for determining the actual volume of action of the assimilation box or the test object within the assimilation box.
The assimilation box system capable of rapidly determining the effective volume and the using method are suitable for an assimilation system with flux characteristics of net respiration, net photosynthesis or zero flux.
Drawings
FIG. 1 is a schematic diagram showing an overall structure of an assimilation box system for rapidly determining an effective volume according to an embodiment of the present invention;
FIG. 2 is an example of a data diagram when the measured object in the assimilation box system is zero flux in accordance with the present invention;
FIG. 3 is an example of a data graph when the measured object in the assimilation box system is net light according to embodiment 1 of the present invention;
fig. 4 is an example of data graph of the measured object in the assimilation box system assimilation box of the present invention in the case of net respiration.
Wherein: 1. CO 22An analyzer; 101. an air filter; 102. CO 22An air intake line; 103. CO 22An air outlet pipeline; 2. an assimilation box; 201. a top plate of the assimilation box; 202. a blending fan; 301. a sensor module; 302. a data acquisition/display module; 401. a pressure balancing tubule; 402. a balloon; 5. a plate penetrating joint; 6. an object to be measured.
Detailed Description
The technical scheme of the invention is more fully explained in detail by combining the attached drawings.
Detailed description of the preferred embodiment 1
Assimilation box system for rapidly determining effective volume, wherein the cylinder comprises assimilation box 2 and CO2The analyzer 1 and the assimilation box top plate 201 are provided with two quick connectors which are respectively connected with CO2 Air intake line 102 and CO2Outlet line 103, inlet line 102 and CO2The end parts of the gas outlet pipelines 103 are respectively connected with CO2The analyzer 1 is fixed. An air filter 101 is also arranged on the air inlet pipe 102 and can filter the air from the assimilation box 2 to the CO2The gas of the analyzer 1 is filtered.
On the top plate 201 of the assimilation boxSetting up CO2Gas injection device, CO2The gas injection device comprises a plate penetrating joint 5 and a gas transmission device, wherein the plate penetrating joint 5 is arranged on the top plate 201, a sealing gasket is fixedly arranged in the plate penetrating joint 5 in the embodiment, the gas transmission device is an injection needle cylinder, and an injection needle penetrates through the sealing gasket in the plate penetrating joint through a thin needle head to finish the quantitative-volume CO2And (5) injecting.
The inside sensor module 301 that has set firmly of 2 lateral walls of assimilation case, sensor module 301's sensor includes atmospheric pressure, humiture, illuminance sensor, and the sensor cable is worn out through the aperture, is connected with the data acquisition/display module 302 who sets firmly at 2 lateral walls of assimilation case, record or demonstration numerical value.
The assimilation box 2 is also provided with a pressure balancing device, the pressure balancing device comprises a pressure balancing tubule 401, one end of the pressure balancing tubule 401 is fixedly arranged on the side wall of the assimilation box 2 and is communicated with the inside of the assimilation box, and the other end of the pressure balancing tubule is connected with a balloon 402.
In this embodiment, the top plate 201 of the assimilation box is a top cover of the assimilation box, and the top cover and the assimilation box are sealed by a sealing strip, a sealant or a water tank.
The inner wall of assimilation roof 201 with wear the board and connect 5 adjacent position installation mixing fan 202, the gaseous quick mixing in the assurance assimilation incasement.
CO in this example2The analyzer is used for detecting CO by a non-dispersive infrared absorption method2And (4) concentration.
The assimilation box is a cylindrical transparent acrylic box with the outer diameter of 20cm m and the outer height of 30cm (comprising a top cover), and the top cover and the box body are sealed by foam cotton; CO adopted2The analyzer comprises a built-in pump, the flow rate is 0.75L/min, and the recording frequency is set to be 2 Hz; the sensor module comprises a temperature and humidity sensor, an air pressure sensor and an illuminance sensor; injected CO2(purity 99.9%, i.e. 0.999 x 10%6ppm) volume was 3.00 ml; the blending fan is a transparent fan with the length of 5 × 1cm, and the fan is fixed by long screws and is about 2cm away from the top cover.
The concrete operation steps of the assimilation box system of the embodiment are as follows:
a) connecting line to CO2The analyzer, the sensor module and the blending fan are electrified and preheated for 10 minutes；
b) Opening a top cover of the assimilation box, putting tested objects (respectively a solid irregular box body, a flowerpot containing plants (simultaneously using a plant growth lamp for light supplement) and a flowerpot without plants) and covering the top cover;
c) observation of CO2Changing the curve, and beginning to record data after balancing for about 30 seconds;
d) after recording the 60s data, CO was injected through the injection hole of the through-plate joint with a syringe2；
e) Recording was continued to approximately 130s data, with visible CO injection2The balance is restored within the last 10 s;
f) performing regression on the data of the first 60s, and using the slope for regression on the balanced data;
g) calculating assimilation Box actual volume and CO2Flux.
The specific calculation steps in this embodiment are as follows:
CO injection2Pre CO2Concentration versus time fit equation: y ═ ax + b1；
CO injection2And the fitting equation after the slope is stable: y ═ ax + b2；
CO2Concentration increment: Δ C ═ b2-b1)；
Assimilation box system actual volume: v ═ V '× C'/[ delta ] C, where CO is injected2Volume V ═ 3.00 ml; CO injection2Volume concentration of (3) C ═ 0.999 x 106ppm；
CO2Flux: flux ═ n × Δ C/. DELTA.t ═ P × V/(R × T) × a, where n is the amount of all gaseous substances in the assimilation box system; v is the actual volume of the assimilation box system; a is the fitted slope (in ppm s)-1) (ii) a Ideal gas constant R-8.314 Pa m3 mol-1K-1(ii) a According to the measurement result, the air pressure P is 84139Pa, and the absolute temperature T is 300.15K;
fig. 2 shows an example of a data diagram when the measured object (solid irregular box) in the assimilation box is zero flux, according to the assimilation box system configuration and measurement parameters used in the present embodiment;
in the figure: v3.00 x 0.999*106/(847.69-502.54)＝8683ml。
Fig. 3 shows an example of a data diagram when measured objects (plant-containing flowerpots and simultaneously supplemented with plant growth lamps) in the assimilation box are net illuminated according to the assimilation box system configuration and measurement parameters used in the present example;
in the figure: v3.00 0.999 106/(1043.09-634.78)＝7340ml＝0.00734m2；
Flux＝84139*0.00734/(8.314*300.15)*(-0.21)＝-0.052μmol CO2 s-1。
FIG. 4 is a view showing an example of a data map when a measured object (plant-free pot) in an assimilation box is net respiration, according to the configuration of the assimilation box system and measurement parameters used in this example;
in the figure: v3.00 0.999 106/(1070.07-656.95)＝7255ml＝0.007255m2；
Flux＝84139*0.007255/(8.314*300.15)*0.14＝0.034μmol CO2 s-1。
It is to be understood that the described embodiments are merely individual embodiments of the invention, rather than all embodiments. All other implementations made by those skilled in the art without any inventive step based on the embodiments of the present invention belong to the protection scope of the present invention.
Claims (10)
1. An assimilation box system for rapidly determining effective volume comprises an assimilation box and CO2An analyzer, the assimilation tank and CO2Passing CO between analyzers2Air inlet line and CO2The gas outlet pipeline communicates, the assimilation incasement sets firmly has sensor module, sensor module connects data acquisition/display module, its characterized in that through the sealed aperture of assimilation case side:
the assimilation box is also provided with a pressure balancing device and CO2A gas injection device;
the CO is2The gas injection device comprises a plate penetrating joint and a gas transmission device, and a sealing gasket is fixedly arranged in the plate penetrating joint;
pressure balance device includes atmospheric pressure balancing tubule, atmospheric pressure balancing tubule one end sets firmly assimilation case lateral wall, with the inside intercommunication of assimilation case, one end is connected with balloon or cushion flask in addition
The sensor of the sensor module comprises an air pressure sensor, a temperature sensor, a humidity sensor and an illuminance sensor.
2. The rapid effective volume determination assimilation box system as claimed in claim 1, wherein: assimilation case is sealed cask shape or square box, establishes the sealed lid that backs down, side opened or under the assimilation case on, seals through sealing strip, sealed glue or basin between sealed lid and the box.
3. The rapid effective volume determination assimilation box system as claimed in claim 1, wherein: the assimilation box top plate is provided with two quick connectors which are respectively connected with the CO2Air inlet line and CO2The air outlet pipeline is connected.
4. The rapid effective volume determination assimilation box system as claimed in claim 1, wherein: the gas transmission device is an injection needle cylinder, and an injection needle passes through a sealing gasket in the plate penetrating joint through a thin needle head to finish the quantitative volume of CO2And (5) injecting.
5. The rapid effective volume determination assimilation box system as claimed in claim 1, wherein: the penetrating plate joint is connected with an extension pipe at a short opening in the assimilation box, and the extension pipe is extended to the bottom of the assimilation box.
6. The rapid effective volume determination assimilation box system as claimed in claim 1, wherein: wear the assimilation incasement wall installation mixing fan of board joint adjacent position, guarantee the quick mixing of the gas in the assimilation incasement.
7. The rapid effective volume determination assimilation box system as claimed in claim 1, wherein the tank system comprises a tank body, a tank cover, a tank bodyIn the following steps: the CO is2The analyzer is used for detecting CO by a non-dispersive infrared absorption method2Concentration, a built-in circulating pump or an external serial circulating pump, and the frequency of sampling record is not lower than 1 Hz.
8. A method of using the rapid effective volume determination assimilation box system of claim 1, wherein: the method comprises the following steps:
a) after all the devices are electrified and preheated, putting the object to be tested into the assimilation box, and sealing the assimilation box;
b) to be treated with CO2After the change slope is stable, starting to record data of the analyzer and the sensor;
c) data were recorded until the slope was stable by CO2Gas injection device for accurately injecting pure CO of known volume2；
d) Continue recording data to CO2The change slope tends to be stable;
e) according to the recorded data, the actual volume of the assimilation box system is calculated in real time by a microcomputer or accurately by manual processing, and the CO of the measured object is further calculated2Flux.
9. The method of using an assimilation box system for rapid determination of effective volume as claimed in claim 8, wherein: CO injection2Assimilation of CO in the front2Concentration of CO close to ambient2Concentration, CO after injection2The concentration increment is between 50 and 500 ppm.
10. The method of using an assimilation box system for rapid determination of effective volume as claimed in claim 8, wherein: CO injection2Pre CO2Concentration versus time was fitted: y ═ ax + b1(ii) a CO injection2And fitting the data after the slope is stable by using the slope before injection: y ═ ax + b2；CO2The increment of the concentration is (b)2-b1)。
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