CN111254229A - Carbon dioxide incubator control method and system and carbon dioxide incubator - Google Patents
Carbon dioxide incubator control method and system and carbon dioxide incubator Download PDFInfo
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
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Abstract
The control method of the carbon dioxide incubator comprises the following steps: acquiring the current temperature and the preset temperature of an inner chamber of a carbon dioxide incubator; outputting indirect heating power of the inner chamber wall according to a preset PID control algorithm; judging whether the temperature difference between the preset temperature and the current temperature belongs to a steady-state interval or not; if the temperature difference between the preset temperature and the current temperature belongs to a steady-state interval, acquiring the minimum power of indirect heating of the inner chamber wall in a first preset time period when the temperature difference belongs to the steady-state interval; generating set power according to the minimum power; adjusting the set power to an initial power for indirect heating of the inner chamber wall in a second preset time period continuous with the first preset time period; wherein the set power is greater than or equal to the minimum power. A control system and a carbon dioxide incubator are also disclosed. After the system enters the steady state operation, the system directly performs pre-intervention on the initial power of the next period according to the current operation state, so that the initiative of temperature control is realized, and the control effect is improved.
Description
Technical Field
The invention belongs to the technical field of culture devices, and particularly relates to a control method and a control system of a carbon dioxide incubator, and the carbon dioxide incubator adopting the control method.
Background
The carbon dioxide incubator is an advanced instrument for culturing cells, tissues and bacteria, and is necessary equipment for developing immunology, oncology, genetics and bioengineering. Is widely applied to the research and production fields of microorganisms, agricultural science and pharmacology. The carbon dioxide incubator is used for culturing cells, tissues and bacteria in vitro by simulating and forming a growth environment similar to the cells, tissues and bacteria in an organism in the incubator body. In general, incubators require stable temperature, stable carbon dioxide levels, constant ph, and high relative saturation humidity.
A common carbon dioxide incubator consists of three main parts, namely an outer shell, a working chamber and a controller. When in use, a carbon dioxide steel cylinder and a carbon dioxide pressure reducing valve are attached. The pure carbon dioxide gas enters the box body after being decompressed and stabilized by the decompression valve. The carbon dioxide incubator adopts indirect heating of the inner chamber wall, and has good temperature uniformity. When the carbon dioxide gas inlet valve is used, a user can set the concentration of carbon dioxide and turn on the carbon dioxide gas inlet switch, namely carbon dioxide enters an inner chamber, and when the concentration of the carbon dioxide reaches a set value, the gas inlet electromagnetic valve is cut off; when the concentration is lower than the set value, the electromagnetic valve is automatically opened to supplement carbon dioxide. When the temperature of the inner chamber reaches a set value and the concentration of carbon dioxide also reaches the requirement, the culture of cells, tissues and bacteria can be carried out. Put into a water tray to ensure that the humidity meets the requirement, and the natural evaporation can generally reach 95 percent.
The carbon dioxide incubator in the prior art adopts a PID control algorithm to control the indirect heating of the inner chamber wall, and more specifically, controls the power of the indirect heating of the inner chamber wall. After the indirect heating of the inner chamber wall is started, the detected temperature gradually approaches to the set temperature. When the temperature deviation in the inner chamber is very small and is close to the set temperature, the integral term in the PID control algorithm is reduced due to the very small deviation, and the output power is small. After the heat stored in the heat-insulating layer of the box body is dissipated, the actual temperature deviates from the set temperature due to the smaller actual power of indirect heating of the inner chamber wall, so that the temperature of the carbon dioxide inner chamber fluctuates, and the working stability of the carbon dioxide incubator is reduced.
Disclosure of Invention
The invention provides a brand-new carbon dioxide incubator control method aiming at the problem that the actual power of indirect heating of the inner chamber wall is low when the temperature of the inner chamber reaches a set value and heat stored in heat insulation cotton of a box body is dissipated, so that the actual temperature deviates from the set temperature and the temperature of the inner chamber fluctuates in the carbon dioxide incubator in the prior art.
A carbon dioxide incubator control method comprises the following steps: acquiring the current temperature and the preset temperature of an inner chamber of a carbon dioxide incubator; outputting indirect heating power of the inner chamber wall according to a preset PID control algorithm; judging whether the temperature difference between the preset temperature and the current temperature belongs to a steady-state interval or not; if the temperature difference between the preset temperature and the current temperature belongs to a steady-state interval, acquiring the minimum power of indirect heating of the inner chamber wall in a first preset time period when the temperature difference belongs to the steady-state interval; generating set power according to the minimum power; adjusting the set power to an initial power for indirect heating of the inner chamber wall in a second preset time period continuous with the first preset time period; wherein the set power is greater than or equal to the minimum power.
Another aspect of the present invention provides a carbon dioxide incubator control system, including a first obtaining module, configured to obtain a current temperature and a preset temperature of an inner chamber of a carbon dioxide incubator; the PID power calculation module is used for outputting the indirect heating power of the inner chamber wall according to a preset PID control algorithm; the judging module is used for judging whether the temperature difference between the preset temperature and the current temperature belongs to a steady-state interval or not; the second acquisition module is used for acquiring the minimum power of indirect heating of the inner chamber wall in a first preset time period under the condition that the temperature difference between the preset temperature and the current temperature belongs to a steady-state interval; the generating module is used for generating set power according to minimum power, wherein the set power is greater than or equal to the minimum power; and the adjusting module is used for adjusting the set power to the initial power for indirectly heating the inner chamber wall in a second preset time period continuous to the first preset time period.
The third aspect of the invention provides a carbon dioxide incubator, which adopts a carbon dioxide incubator control method, and the control method comprises the following steps: acquiring the current temperature and the preset temperature of an inner chamber of a carbon dioxide incubator; outputting indirect heating power of the inner chamber wall according to a preset PID control algorithm; judging whether the temperature difference between the preset temperature and the current temperature belongs to a steady-state interval or not; if the temperature difference between the preset temperature and the current temperature belongs to a steady-state interval, acquiring the minimum power of indirect heating of the inner chamber wall in a first preset time period when the temperature difference belongs to the steady-state interval; generating set power according to the minimum power; adjusting the set power to an initial power for indirect heating of the inner chamber wall in a second preset time period continuous with the first preset time period; wherein the set power is greater than or equal to the minimum power.
Compared with the prior art, the invention has the advantages and positive effects that: when the carbon dioxide incubator enters a first stable operation period, active intervention is introduced, the minimum power in a first preset time period is firstly obtained, set power which is larger than or equal to the minimum power is generated according to the minimum power and serves as the initial power of the next period, and output power of a PID algorithm is compensated in a continuous second preset time period. Therefore, even if the power output by the PID is smaller due to smaller deviation and deviation introduced by the heat insulation layer in the second preset time period, the output power cannot be continuously reduced in the first preset time period and the second preset time period due to the preposed intervention compensation, and the time-varying property and the hysteresis property in the temperature control process are overcome; for the second preset time period, the preposed active intervention has the advantage of high control speed, and the preposed intervention is directly carried out on the output parameters according to the current state, so that the initiative of temperature control is really realized, and the control effect of the temperature control of the carbon dioxide incubator is improved.
Other features and advantages of the present invention will become more apparent from the following detailed description of the invention when taken in conjunction with the accompanying drawings.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the embodiments are briefly introduced below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without creative efforts.
FIG. 1 is a flow chart of a first embodiment of a method for controlling a carbon dioxide incubator according to the present invention;
FIG. 2 is a flow chart of a second embodiment of a method for controlling a carbon dioxide incubator according to the present invention;
FIG. 3 is a flow chart of a method of generating a set power in the carbon dioxide incubator control method shown in FIG. 1 or FIG. 2;
FIG. 4 is a block diagram schematically illustrating the structure of a carbon dioxide incubator control system according to the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention will be described in further detail with reference to the accompanying drawings and examples.
The terms "first," "second," "third," and the like in the description and in the claims, and in the drawings, are used for distinguishing between different objects and not necessarily for describing a particular sequential or chronological order. Furthermore, the terms "comprising" and "having," as well as any variations thereof, are intended to cover a non-exclusive inclusion. For example, a process, method, system, article, or apparatus that comprises a list of steps or elements is not limited to only those steps or elements listed, but may alternatively include other steps or elements not listed, or inherent to such process, method, article, or apparatus.
Reference throughout this specification to "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment can be included in at least one embodiment of the present application. The appearances of the phrase in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments. One skilled in the art will appreciate that the embodiments described herein can be combined with other embodiments.
The carbon dioxide incubator is a biochemical experimental instrument for cell tissue and bacteria culture. The carbon dioxide incubator comprises an incubator body, and a door body is arranged on the front side of the incubator body. The door body can be attached to the box body through a magnetic sealing strip, and can also be pivoted with the box body through other connecting structures. An inner chamber is formed in the box body, and the inner chamber wall of the inner chamber and the shelf in the inner chamber are both preferably made of stainless steel and subjected to electrolytic polishing treatment. The carbon dioxide concentration in the inner chamber, the temperature and humidity of the inner chamber are controlled to stabilize the culture environment of the culture. The outer side of the inner chamber is provided with a heat preservation layer.
The heating system of the carbon dioxide incubator, i.e. the indirect heating of the inner chamber wall, is mainly concentrated near the inner chamber, which is used to keep the temperature in the inner chamber within a desired range of values. The inner chamber wall indirect heating mainly comprises a main body heating wire, a door body heating wire and a cabinet opening heating wire, wherein the main body heating wire comprises heating wires attached to the side wall of the box body, and the heating wires are respectively arranged on the outer surface of the bottom wall, the outer surface of the top wall, the outer surface of the rear wall, the outer surface of the left side and the outer surface of the right side of the inner chamber. The heating wire adopts the electric heating principle.
Since the environment of the inner chamber needs to be kept stable in most cases, in order to facilitate the experimenter to observe the state change of the biological tissues in the box body, an operable sealing door is preferably arranged between the door body and the inner chamber, and the sealing door can be made of tempered glass. Because the cabinet opening heating wire and the door body heating wire are arranged, condensed water cannot appear on the sealing door made of toughened glass, and the perspective effect of the sealing door can be kept even if the temperature of the inner chamber is higher. The humidity in the carbon dioxide incubator is relatively high, and under the condition of normal operation, the relative humidity of the inner chamber is more than or equal to 90%. To maintain a higher humidity level, distilled water in a vessel, such as a humidification tray, disposed in the internal chamber evaporates and maintains the internal chamber relative humidity. Because the internal chamber wall indirect heating pastes the outer surface of the bottom wall, the outer surface of the top wall, the outer surface of the rear wall, the outer surface of the left side and the outer surface of the right side of the internal chamber respectively, and is arranged on the door body and the cabinet opening, even in an evaporation state, condensed water can not be formed on the top wall of the internal chamber, thereby improving the stability of the equipment and avoiding the occurrence of pollution.
Optionally, a fan may be provided at the rear of the cabinet to keep the chamber air circulating so that the gas is distributed as evenly as possible. The sealing door is preferably provided with a Hall sensor, when the opening of the sealing door is detected, a sealing door state signal is output to a controller of the carbon dioxide incubator, the controller shuts off the air source and the heating system, and the fan is turned off simultaneously, so that the phenomenon that the gas concentration in the inner chamber is too high or heating is out of control is avoided.
The controller of the carbon dioxide incubator is preferably arranged at the top of the box body, and can also be realized by a processor which is externally arranged on the carbon dioxide incubator. The temperature of the inner chamber is preferably detected by a temperature sensor consisting of a platinum Pt1000 resistor. Specifically, the temperature sensor can directly transmit the sampled inner chamber temperature data to the controller of the carbon dioxide incubator in a serial communication mode, and can also transmit the sampled data to a personal computer, a remote server, a handheld device, a smart phone and/or a wearable device in a wireless communication mode, and the devices further transmit the sampled data to the controller of the carbon dioxide incubator; or the controller of the carbon dioxide incubator transmits the sampled data to a personal computer, remote server, handheld device, smartphone, and/or wearable device. The wireless communication may be in a one-to-one communication mode, or through one or more servers in a local area network, or through a cloud server. In this way, the devices can each obtain temperature detection data. Besides, if the phenomenon out of control appears in inner room temperature or pressure, warning signal can also be received to these equipment, and the form of concrete embodiment includes that there is the sign that exceeds preset critical state to light and twinkle on the display screen, and the mobile phone APP of experimenter receives the warning, and the sign on the carbon dioxide incubator lights and twinkles to and voice broadcast etc..
The temperature in the inner chamber of the carbon dioxide incubator needs to be accurately controlled, so that the success rate and the efficiency of culturing biological cells, tissues and the like are effectively improved. In order to solve the problem that the temperature of the inner chamber fluctuates due to the fact that the output power is low caused by the fact that an integral term in a PID control algorithm is small when a carbon dioxide incubator enters a steady-state operation mode because the PID control algorithm is adopted to control the indirect heating power of the inner chamber wall. A control method of a completely new carbon dioxide incubator is shown in figure 1. This control method specifically includes the following steps.
S101, obtaining the current temperature and the preset temperature of the inner chamber of the carbon dioxide incubator.
And S102, outputting indirect heating power of the inner chamber wall according to a preset PID control algorithm.
S103, judging whether the temperature difference between the preset temperature and the current temperature belongs to a steady-state interval or not.
The current temperature is detected through a platinum Pt1000 resistor, and the preset temperature is set by experimenters according to specific experimental requirements. When the temperature difference between the real-time temperature (namely the current temperature) and the preset temperature is large, the carbon dioxide incubator is heated by the power output by the preset PID control algorithm, so that the speed of the current temperature approaching the preset temperature is accelerated. Meanwhile, the real-time temperature is monitored according to a set sampling period, and if the current temperature reaches a certain threshold value, the steady-state interval is entered. In order to avoid the PID algorithm from outputting heating power according to a small temperature difference in a steady-state interval to cause temperature fluctuation of an inner chamber after heat dissipation of a heat insulation layer, in the method provided by the embodiment, after steady-state operation is performed, active intervention is performed to change a control mode of indirect heating power of an inner chamber wall.
Specifically, the method comprises the following steps:
s104, if the temperature difference between the preset temperature and the current temperature belongs to a steady-state interval, acquiring the minimum power of indirect heating of the inner chamber wall in the first preset time period under the condition that the temperature difference belongs to the steady-state interval.
And S105, generating set power according to the minimum power, wherein the set power is greater than or equal to the minimum power.
S106, adjusting the set power to the initial power for indirectly heating the inner chamber wall in a second preset time period continuous to the first preset time period.
In steps S104 to S106, that is, when the temperature difference between the preset temperature and the current temperature belongs to the steady-state interval, it can be regarded that the carbon dioxide incubator enters the first steady operation period. At the moment, active intervention is introduced, the minimum power in the first preset time period is firstly obtained, and the set power which is larger than or equal to the minimum power is generated according to the minimum power and is used as the initial power of the next cycle, namely the output power of the PID algorithm is compensated in the continuous second preset time period. Therefore, even if the power output by the PID is smaller due to smaller deviation and deviation introduced by the heat insulation layer in the second preset time period, the output power cannot be continuously reduced in the first preset time period and the second preset time period due to the preposed intervention compensation, and the time-varying property and the hysteresis property in the temperature control process are overcome; for the second preset time period, the preposed active intervention has the advantage of high control speed, and the preposed intervention is directly carried out on the output parameters according to the current state, so that the initiative of temperature control is really realized, and the control effect of the temperature control of the carbon dioxide incubator is improved.
It should be noted that, since the integral term in the PID control algorithm is smaller when the temperature difference is smaller, the output power is likely to be smaller, and therefore, in order to improve the control effect, the initial power of the second preset period needs to be greater than the minimum power in the first preset period in most cases. However, if the environmental parameters in the chamber are stable for the first predetermined period of time, it may happen that the power for indirect heating of the chamber wall is exactly the same for the entire first predetermined period of time. If the indirect heating power of the inner chamber wall is completely the same in a complete first preset time period and the temperature difference between the current temperature and the preset temperature is in a steady state interval, the carbon dioxide incubator is in a stable working state, namely the minimum power is directly taken as the set power, and the set power is adjusted to the initial power for indirect heating of the inner chamber wall in a second preset time period continuous with the first preset time period, so that the overshoot of the temperature is avoided.
In most cases, even if the temperature difference belongs to a steady-state interval condition due to the change of heat radiation and the external environment, the power for indirectly heating the inner chamber wall fluctuates due to the adjustment effect of the PID control in the continuous operation for the first preset period, the correction power prestored in advance can be called from the controller in consideration of the adjustment result with a smaller integral term according to the minimum power generation setting power, and the sum of the correction power and the minimum power is taken as the setting power. The generation of the set power from the correction power depends on the accuracy of the correction power, and is prone to fluctuation. Therefore, as shown in fig. 3, generating the set power according to the minimum power preferably includes the steps of:
s1051, obtaining the maximum power of indirect heating of the inner chamber wall in a first preset time period when the temperature difference belongs to a steady state interval condition.
S1052, setting the power equal to the average of the minimum power and the maximum power.
In the mode, the power of the indirect heating of the inner chamber wall in the first preset time period is actually considered, the target optimal control of the indirect heating power of the inner chamber wall is realized by utilizing the average value of the power of the indirect heating of the inner chamber wall in the working state of the steady-state interval, and the control method can effectively avoid the temperature runaway condition. The first preset time period can be regarded as a first stable operation period of the carbon dioxide incubator, the average power calculated by the first stable operation period is used as the initial power of the next period, and circulation is performed in this way to compensate the output power in the PID algorithm, so that the output power cannot be continuously reduced. At the beginning of each second preset period, the initial power of the indirect heating power of the inner chamber wall is maintained at the average power, which is equivalent to "preheating" the carbon dioxide incubator, preventing the temperature in the inner chamber from fluctuating.
Referring to fig. 3, in the second preset period, the control method further includes the steps of:
and S207, judging whether the temperature difference between the preset temperature and the current temperature belongs to a steady-state interval.
S208, if the temperature difference between the preset temperature and the current temperature exceeds the steady-state interval, the indirect heating power of the inner chamber wall is output according to a preset PID control algorithm, the average value is not used as the initial power for the indirect heating of the inner chamber wall in the second preset time period, so that the system has good anti-interference performance, and when the external environment changes rapidly, the system can respond rapidly to avoid further expansion of fluctuation.
It should be noted that the steady-state interval means that the current temperature falls within the range of [ set temperature-0.1 ℃, set temperature +0.1 ℃), and the first preset time period is set to 1 hour.
As shown in fig. 4, another aspect of the present invention provides a carbon dioxide incubator control system, which specifically includes:
the first acquisition module 11 is used for acquiring the current temperature and the preset temperature of the inner chamber of the carbon dioxide incubator. The preset temperature is set by an experimenter according to specific requirements, and the current temperature is detected by a temperature sensor consisting of a platinum Pt1000 resistor.
And the PID power calculation module 12 is used for outputting the internal chamber wall indirect heating power according to a preset PID control algorithm by the PID power calculation module 12.
And the judging module 13 is used for judging whether the temperature difference between the preset temperature and the current temperature belongs to a steady-state interval or not.
When the temperature difference between the real-time temperature (i.e. the current temperature) and the preset temperature is large, the control system outputs the indirect heating power of the inner chamber wall to operate by using the PID power calculation module 12 according to the preset PID control algorithm, so as to accelerate the speed of the current temperature of the inner chamber approaching the preset temperature. And simultaneously monitoring the real-time temperature according to a set sampling period. And if the current temperature reaches a certain threshold value, determining that the temperature of the inner chamber enters a steady-state interval. In order to avoid the fluctuation of the temperature of the inner chamber, after entering a steady-state interval, the control mode of the indirect heating power of the inner chamber wall is changed.
The control system is also specially designed with: and the second obtaining module 14 is configured to obtain, when the temperature difference between the preset temperature and the current temperature belongs to a steady-state interval, a minimum power for indirectly heating the inner chamber wall in a first preset time period when the temperature difference belongs to the steady-state interval. A generating module 15, where the generating module 15 is configured to generate a set power according to a minimum power, and the set power is greater than or equal to the minimum power. An adjusting module 16, wherein the adjusting module 16 is configured to adjust the set power to an initial power for indirect heating of the inner chamber wall in a second predetermined time period consecutive to the first predetermined time period. In order to avoid the fluctuation of the temperature of the inner chamber, when the temperature difference between the preset temperature and the current temperature belongs to a steady-state interval, the control system introduces active intervention, generates set power which is greater than or equal to the minimum power according to the minimum power, and overcomes the time-varying property and the hysteresis property in the possible temperature control process. For the second preset time period after the system is stabilized, the preposed active intervention has the advantage of high control speed, and the preposed intervention is directly carried out on the output parameters according to the current state, so that the initiative of temperature control is really realized, and the control effect of the temperature control of the carbon dioxide incubator is improved.
It should be noted that, since the integral term in the PID control algorithm is smaller when the temperature difference is smaller, the output power is likely to be smaller, and therefore, in order to improve the control effect, the initial power in the second preset period is usually required to be greater than the minimum power in the first preset period. However, if the environmental parameters in the chamber are stable for the first predetermined period of time, it may happen that the power for indirect heating of the chamber wall is exactly the same for the entire first predetermined period of time. If the indirect heating power of the inner chamber wall is completely the same in a complete first preset time period and the temperature difference between the current temperature and the preset temperature is in a steady state interval, the carbon dioxide incubator is in a stable working state, namely the minimum power is directly taken as the set power, and the set power is adjusted to the initial power for indirect heating of the inner chamber wall in a second preset time period continuous with the first preset time period, so that the overshoot of the temperature is avoided.
In most cases, even if the temperature difference belongs to a steady-state interval condition due to the change of heat radiation and the external environment, the power for indirectly heating the inner chamber wall fluctuates due to the adjustment effect of the PID control in the continuous operation for the first preset period, the correction power prestored in advance can be called from the controller in consideration of the adjustment result with a smaller integral term according to the minimum power generation setting power, and the sum of the correction power and the minimum power is taken as the setting power. The generation of the set power from the correction power depends on the accuracy of the correction power, and is prone to fluctuation. Therefore, preferably, the generating module includes: the sampling unit is used for acquiring the maximum power of indirect heating of the inner chamber wall in a first preset time period when the temperature difference belongs to a steady-state interval condition; and a calculation unit for calculating the set power, wherein the set power is equal to an average of the minimum power and the maximum power.
The generation module actually considers the power of the indirect heating of the inner chamber wall in the first preset time period, the target optimization control of the indirect heating power of the inner chamber wall is realized by using the average value of the power of the indirect heating of the inner chamber wall in the working state of the steady-state interval, and the control method can effectively avoid the temperature out-of-control condition. The first preset time period can be regarded as a first stable operation period of the carbon dioxide incubator, the average power calculated by the first stable operation period is used as the initial power of the next period, and circulation is performed in this way to compensate the output power in the PID algorithm, so that the output power cannot be continuously reduced. At the beginning of each second preset period, the initial power of the indirect heating power of the inner chamber wall is maintained at the average power, which is equivalent to "preheating" the carbon dioxide incubator, preventing the temperature in the inner chamber from fluctuating.
The control system is also preferably provided with a correction module, and the correction module is used for judging whether the temperature difference between the preset temperature and the current temperature belongs to a steady-state interval or not in a second preset time period; and if the temperature difference does not belong to the steady-state interval, outputting the indirect heating power of the inner chamber wall according to a preset PID control algorithm. Therefore, the system has good anti-interference performance, and can quickly respond to the external environment when the external environment changes rapidly, so that the fluctuation is prevented from further expanding.
It should be noted that the steady-state interval means that the current temperature falls within the range of [ set temperature-0.1 ℃, set temperature +0.1 ℃), and the first preset time period is set to 1 hour.
The embodiment of the application also provides a carbon dioxide incubator, and the control method of the carbon dioxide incubator is applied. The specific steps of the carbon dioxide incubator control method are described in the detailed description of the above embodiments and the detailed description of the drawings in the specification. The details are not repeated herein, and the carbon dioxide incubator adopting the carbon dioxide incubator control method can achieve the same technical effects. The indirect heating of the inner chamber wall of the carbon dioxide incubator comprises a main body heating wire, a door body heating wire and a cabinet opening heating wire; the main heating wire comprises a heating wire attached to the side wall of the box body, a heating wire attached to the top wall of the box body and a heating wire attached to the bottom wall of the box body.
Embodiments of the present application also provide a computer storage medium, wherein the computer storage medium stores a computer program for electronic data exchange, the computer program causing a carbon dioxide incubator to perform part or all of the steps of any one of the methods as set forth in the above method embodiments.
In the above embodiments, the descriptions of the respective embodiments have respective emphasis, and for parts that are not described in detail in a certain embodiment, reference may be made to related descriptions of other embodiments.
In the embodiments provided in the present application, it should be understood that the disclosed apparatus may be implemented in other manners. For example, the above-described apparatus embodiments are merely illustrative, and for example, the division of the above-described units or modules is only one type of division of logical functions, and there may be other divisions when actually implemented, for example, a plurality of units or components may be combined or integrated into another system, or some features may be omitted, or not executed. In addition, the shown or discussed mutual coupling or direct coupling or communication connection may be an indirect coupling or communication connection through some interfaces, devices or units, and may be an electrical or other form.
The units described as the separate components may or may not be physically separate, and the components displayed as the units may or may not be physical units, that is, may be located in one physical space, or may also be distributed on a plurality of network units, and some or all of the units may be selected according to actual needs to achieve the purpose of the embodiment.
In addition, functional units in the embodiments of the present application may be integrated into one processing unit, or each unit may exist alone physically, or two or more units are integrated into one unit. The integrated unit can be realized in a form of hardware, and can also be realized in a form of a software functional unit.
The above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that various changes may be made and equivalents may be substituted for elements thereof; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions.
Claims (10)
1. A control method of a carbon dioxide incubator is characterized by comprising the following steps:
acquiring the current temperature and the preset temperature of an inner chamber of a carbon dioxide incubator;
outputting indirect heating power of the inner chamber wall according to a preset PID control algorithm;
judging whether the temperature difference between the preset temperature and the current temperature belongs to a steady-state interval or not;
if the temperature difference between the preset temperature and the current temperature belongs to a steady-state interval, acquiring the minimum power of indirect heating of the inner chamber wall in a first preset time period when the temperature difference belongs to the steady-state interval;
generating set power according to the minimum power;
adjusting the set power to an initial power for indirect heating of the inner chamber wall in a second preset time period continuous with the first preset time period;
wherein the set power is greater than or equal to the minimum power.
2. The carbon dioxide incubator control method according to claim 1, characterized by further comprising the steps of:
generating the set power according to the minimum power comprises the following steps:
obtaining the maximum power of indirect heating of the inner chamber wall in a first preset time period when the temperature difference belongs to a steady state interval condition;
the set power is equal to an average of the minimum power and the maximum power.
3. The carbon dioxide incubator control method according to claim 2,
judging whether the temperature difference between the preset temperature and the current temperature belongs to a steady-state interval or not in the second preset time period;
and if the temperature does not belong to the steady-state interval, outputting the inter-chamber wall heating power according to a preset PID control algorithm.
4. The carbon dioxide incubator control method according to claim 3,
the first predetermined period of time is 1 hour.
5. A carbon dioxide incubator control system, comprising:
the first acquisition module is used for acquiring the current temperature and the preset temperature of the inner chamber of the carbon dioxide incubator;
the PID power calculation module is used for outputting the indirect heating power of the inner chamber wall according to a preset PID control algorithm;
the judging module is used for judging whether the temperature difference between the preset temperature and the current temperature belongs to a steady-state interval or not;
the second acquisition module is used for acquiring the minimum power of indirect heating of the inner chamber wall in a first preset time period under the condition that the temperature difference between the preset temperature and the current temperature belongs to a steady-state interval;
the generating module is used for generating set power according to minimum power, wherein the set power is greater than or equal to the minimum power; and
an adjustment module for adjusting the set power to an initial power for indirect heating of the interior chamber wall in a second predetermined time period that is continuous with the first predetermined time period.
6. The carbon dioxide incubator control method according to claim 5,
the generation module comprises:
the sampling unit is used for acquiring the maximum power of indirect heating of the inner chamber wall in a first preset time period when the temperature difference belongs to a steady-state interval condition; and
a calculation unit for calculating the set power, wherein the set power is equal to an average of the minimum power and the maximum power.
7. The carbon dioxide incubator control method according to claim 6, further comprising:
the correction module is used for judging whether the temperature difference between the preset temperature and the current temperature belongs to a steady-state interval or not in a second preset time period; and if the temperature difference does not belong to the steady-state interval, outputting the indirect heating power of the inner chamber wall according to a preset PID control algorithm.
8. The carbon dioxide incubator control method according to claim 7,
the first predetermined period of time is 1 hour.
9. A carbon dioxide incubator, characterized in that the control method according to any one of claims 1 to 4 is employed.
10. The carbon dioxide incubator according to claim 9,
the inner chamber wall indirect heating comprises a main body heating wire, a door body heating wire and a cabinet opening heating wire; the main heating wire comprises a heating wire attached to the side wall of the box body, a heating wire attached to the top wall of the box body and a heating wire attached to the bottom wall of the box body.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113549713A (en) * | 2021-07-05 | 2021-10-26 | 青岛海特生物医疗有限公司 | Control method and device for carbon dioxide incubator and carbon dioxide incubator |
| CN113667600A (en) * | 2021-07-21 | 2021-11-19 | 青岛海特生物医疗有限公司 | Method and device for distributing power of carbon dioxide incubator and carbon dioxide incubator |
| CN113801967A (en) * | 2021-09-09 | 2021-12-17 | 青岛海尔生物医疗科技有限公司 | Method and device for regulating and controlling temperature of incubator and incubator |
| CN114438035A (en) * | 2022-01-20 | 2022-05-06 | 冰山松洋生物科技(大连)有限公司 | CO (carbon monoxide)2Method for controlling culture temperature of IPS cells in incubator |
| WO2022156642A1 (en) * | 2021-01-21 | 2022-07-28 | 青岛海尔生物医疗股份有限公司 | Control method for rewarming of constant temperature incubator, and constant temperature incubator |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040122559A1 (en) * | 1998-03-23 | 2004-06-24 | Cepheid | System and method for temperature control |
| US20140255910A1 (en) * | 2011-10-10 | 2014-09-11 | DASGIP Information and Process Technology GmbH | Method for controlled operation of a biotechnological apparatus and bioreactor system |
| CN104862227A (en) * | 2014-02-20 | 2015-08-26 | 中国科学院广州生物医药与健康研究院 | Biological incubator and parameter control apparatus thereof |
| CN106190835A (en) * | 2016-07-13 | 2016-12-07 | 天津卫凯生物工程有限公司 | A kind of filling type cell culture system and method |
-
2020
- 2020-02-25 CN CN202010116413.1A patent/CN111254229B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040122559A1 (en) * | 1998-03-23 | 2004-06-24 | Cepheid | System and method for temperature control |
| US20140255910A1 (en) * | 2011-10-10 | 2014-09-11 | DASGIP Information and Process Technology GmbH | Method for controlled operation of a biotechnological apparatus and bioreactor system |
| CN104862227A (en) * | 2014-02-20 | 2015-08-26 | 中国科学院广州生物医药与健康研究院 | Biological incubator and parameter control apparatus thereof |
| CN106190835A (en) * | 2016-07-13 | 2016-12-07 | 天津卫凯生物工程有限公司 | A kind of filling type cell culture system and method |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022156642A1 (en) * | 2021-01-21 | 2022-07-28 | 青岛海尔生物医疗股份有限公司 | Control method for rewarming of constant temperature incubator, and constant temperature incubator |
| CN113549713A (en) * | 2021-07-05 | 2021-10-26 | 青岛海特生物医疗有限公司 | Control method and device for carbon dioxide incubator and carbon dioxide incubator |
| WO2023279952A1 (en) * | 2021-07-05 | 2023-01-12 | 青岛海尔生物医疗科技有限公司 | Control method and apparatus for carbon dioxide incubator, and carbon dioxide incubator |
| CN113667600A (en) * | 2021-07-21 | 2021-11-19 | 青岛海特生物医疗有限公司 | Method and device for distributing power of carbon dioxide incubator and carbon dioxide incubator |
| WO2023000901A1 (en) * | 2021-07-21 | 2023-01-26 | 青岛海尔生物医疗科技有限公司 | Method and device for power distribution in carbon dioxide incubator, and carbon dioxide incubator |
| CN113667600B (en) * | 2021-07-21 | 2023-08-11 | 青岛海特生物医疗有限公司 | Method and device for distributing power of carbon dioxide incubator and carbon dioxide incubator |
| CN113801967A (en) * | 2021-09-09 | 2021-12-17 | 青岛海尔生物医疗科技有限公司 | Method and device for regulating and controlling temperature of incubator and incubator |
| WO2023035811A1 (en) * | 2021-09-09 | 2023-03-16 | 青岛海尔生物医疗科技有限公司 | Method and device for regulating temperature of incubator, and incubator |
| CN113801967B (en) * | 2021-09-09 | 2024-03-15 | 青岛海尔生物医疗科技有限公司 | Method and device for regulating temperature of incubator and incubator |
| CN114438035A (en) * | 2022-01-20 | 2022-05-06 | 冰山松洋生物科技(大连)有限公司 | CO (carbon monoxide)2Method for controlling culture temperature of IPS cells in incubator |
| CN114438035B (en) * | 2022-01-20 | 2023-10-20 | 冰山松洋生物科技(大连)有限公司 | CO (carbon monoxide) 2 Culture temperature control method of IPS cells of incubator |
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