CN111246936A - 用于核酸合成的微流控芯片 - Google Patents

用于核酸合成的微流控芯片 Download PDF

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CN111246936A
CN111246936A CN201880068740.5A CN201880068740A CN111246936A CN 111246936 A CN111246936 A CN 111246936A CN 201880068740 A CN201880068740 A CN 201880068740A CN 111246936 A CN111246936 A CN 111246936A
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reaction chamber
input
microchannel
reaction
microchannels
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CN111246936B (zh
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孙宝策
王勇
黄小罗
陈泰
谭宏东
沈玥
徐讯
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BGI Shenzhen Co Ltd
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BGI Shenzhen Co Ltd
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J14/00—Chemical processes in general for reacting liquids with liquids; Apparatus specially adapted therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical

Abstract

用于合成核酸的微流控芯片，其包括：反应室，与所述反应室的入口连接的一个或多个输入微通道，与所述反应室的出口连接的输出微通道，以及用于控制输入微通道的流体输入的至少一个阀门、用于控制进入和/或流出所述反应室的流体流动的至少一个阀门和用于控制输出微通道的流体输出的至少一个阀门。以及微流控芯片的微流控系统、使用其的方法和用途。

Description

PCT国内申请，说明书已公开。

Claims (12)

  1. PCT国内申请，权利要求书已公开。
CN201880068740.5A 2017-10-25 2018-10-08 用于核酸合成的微流控芯片 Active CN111246936B (zh)

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CN201711015721X 2017-10-25
CN201711015721 2017-10-25
PCT/CN2018/109278 WO2019080704A1 (zh) 2017-10-25 2018-10-08 用于核酸合成的微流控芯片

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CN111246936A true CN111246936A (zh) 2020-06-05
CN111246936B CN111246936B (zh) 2022-05-03

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114621307A (zh) * 2022-04-12 2022-06-14 中国科学院苏州生物医学工程技术研究所 一种寡核苷酸空间坐标编码方法及其微流控装置
CN114904469A (zh) * 2021-02-09 2022-08-16 深圳华大生命科学研究院 合成装置、合成仪及合成方法
CN114985023A (zh) * 2021-10-27 2022-09-02 北京擎科生物科技有限公司 微阵列芯片和包括其的寡核苷酸合成装置
WO2023116712A1 (zh) * 2021-12-20 2023-06-29 英诺维尔智能科技（苏州）有限公司 一种高通量核酸合成芯片及其使用方法

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* Cited by examiner, † Cited by third party
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US11578348B2 (en) * 2019-04-24 2023-02-14 The University Of Massachusetts Enzymatic methods to generate high yields of sequence specific rna with extreme precision

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060160206A1 (en) * 2002-12-02 2006-07-20 Gyros Ab Parallel prosessing of microfluidic devices
CN101611048A (zh) * 2006-12-12 2009-12-23 综合Dna技术公司 含有高浓度鸟嘌呤单体的寡核苷酸
EP2626133A1 (en) * 2012-02-10 2013-08-14 Chemtrix B.V. Micro-fluidic system
CN106065391A (zh) * 2016-07-20 2016-11-02 国家纳米科学中心 用于单细胞分选和单细胞全基因组扩增的微流控芯片

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DE10350484B4 (de) * 2003-12-20 2006-02-02 Ehrfeld Mikrotechnik Bts Gmbh Verfahren und Vorrichtung zur Markierung von Proteinen
CN102533738B (zh) * 2012-03-15 2013-07-31 田敬东 一种基因合成方法、基因芯片及试剂盒
CN104914102B (zh) * 2015-06-15 2017-06-27 江苏大学 一种微流控芯片及其用途
CN205443311U (zh) * 2016-01-28 2016-08-10 上海美吉逾华生物医药科技有限公司 一种进行固相pcr反应的自动化微流工作站

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060160206A1 (en) * 2002-12-02 2006-07-20 Gyros Ab Parallel prosessing of microfluidic devices
CN101611048A (zh) * 2006-12-12 2009-12-23 综合Dna技术公司 含有高浓度鸟嘌呤单体的寡核苷酸
EP2626133A1 (en) * 2012-02-10 2013-08-14 Chemtrix B.V. Micro-fluidic system
CN106065391A (zh) * 2016-07-20 2016-11-02 国家纳米科学中心 用于单细胞分选和单细胞全基因组扩增的微流控芯片

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
赵亮、申洁、周宏伟等: "集成微流控芯片", 《科学通报》 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114904469A (zh) * 2021-02-09 2022-08-16 深圳华大生命科学研究院 合成装置、合成仪及合成方法
CN114985023A (zh) * 2021-10-27 2022-09-02 北京擎科生物科技有限公司 微阵列芯片和包括其的寡核苷酸合成装置
CN114985023B (zh) * 2021-10-27 2024-01-05 北京擎科生物科技股份有限公司 微阵列芯片和包括其的寡核苷酸合成装置
WO2023116712A1 (zh) * 2021-12-20 2023-06-29 英诺维尔智能科技（苏州）有限公司 一种高通量核酸合成芯片及其使用方法
CN114621307A (zh) * 2022-04-12 2022-06-14 中国科学院苏州生物医学工程技术研究所 一种寡核苷酸空间坐标编码方法及其微流控装置

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CN111246936B (zh) 2022-05-03

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