CN111235062B - Compound microbial agent for treating COD (chemical oxygen demand) pollutants in nonmetal organic synthetic tanning agent wastewater and application thereof - Google Patents
Compound microbial agent for treating COD (chemical oxygen demand) pollutants in nonmetal organic synthetic tanning agent wastewater and application thereof Download PDFInfo
- Publication number
- CN111235062B CN111235062B CN202010137051.4A CN202010137051A CN111235062B CN 111235062 B CN111235062 B CN 111235062B CN 202010137051 A CN202010137051 A CN 202010137051A CN 111235062 B CN111235062 B CN 111235062B
- Authority
- CN
- China
- Prior art keywords
- wastewater
- cod
- bacillus
- dichloro
- syntan
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000003795 chemical substances by application Substances 0.000 title claims abstract description 80
- 239000002351 wastewater Substances 0.000 title claims abstract description 62
- 230000000813 microbial effect Effects 0.000 title claims abstract description 44
- 229910052755 nonmetal Inorganic materials 0.000 title claims abstract description 39
- 150000001875 compounds Chemical class 0.000 title claims abstract description 27
- 239000003344 environmental pollutant Substances 0.000 title claims abstract description 15
- 231100000719 pollutant Toxicity 0.000 title claims abstract description 15
- 239000000126 substance Substances 0.000 title claims description 7
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 title claims description 6
- 229910052760 oxygen Inorganic materials 0.000 title claims description 6
- 239000001301 oxygen Substances 0.000 title claims description 6
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 44
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 44
- 238000000855 fermentation Methods 0.000 claims abstract description 41
- 230000004151 fermentation Effects 0.000 claims abstract description 41
- 241000193408 Bacillus badius Species 0.000 claims abstract description 29
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 28
- OMRXVBREYFZQHU-UHFFFAOYSA-N 2,4-dichloro-1,3,5-triazine Chemical compound ClC1=NC=NC(Cl)=N1 OMRXVBREYFZQHU-UHFFFAOYSA-N 0.000 claims abstract description 25
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical group NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims abstract description 11
- 230000015556 catabolic process Effects 0.000 claims abstract description 11
- 238000006731 degradation reaction Methods 0.000 claims abstract description 11
- IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical group O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 claims abstract description 10
- MZSDGDXXBZSFTG-UHFFFAOYSA-M sodium;benzenesulfonate Chemical group [Na+].[O-]S(=O)(=O)C1=CC=CC=C1 MZSDGDXXBZSFTG-UHFFFAOYSA-M 0.000 claims abstract description 9
- 241000193469 Clostridium pasteurianum Species 0.000 claims abstract description 7
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 54
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 claims description 30
- 239000011780 sodium chloride Substances 0.000 claims description 27
- 150000004893 oxazines Chemical class 0.000 claims description 21
- 238000012258 culturing Methods 0.000 claims description 20
- 235000019270 ammonium chloride Nutrition 0.000 claims description 15
- 241000606860 Pasteurella Species 0.000 claims description 11
- 241000193395 Sporosarcina pasteurii Species 0.000 claims description 11
- 239000002054 inoculum Substances 0.000 claims description 11
- 239000000356 contaminant Substances 0.000 claims description 6
- 239000001963 growth medium Substances 0.000 abstract description 51
- 239000002957 persistent organic pollutant Substances 0.000 abstract description 26
- 239000002131 composite material Substances 0.000 abstract description 18
- 244000005700 microbiome Species 0.000 abstract description 4
- 150000003918 triazines Chemical class 0.000 abstract description 3
- 239000001888 Peptone Substances 0.000 description 36
- 108010080698 Peptones Proteins 0.000 description 36
- 235000015278 beef Nutrition 0.000 description 36
- 235000019319 peptone Nutrition 0.000 description 36
- 239000002068 microbial inoculum Substances 0.000 description 30
- 238000009630 liquid culture Methods 0.000 description 22
- 239000002609 medium Substances 0.000 description 21
- 238000000034 method Methods 0.000 description 17
- 239000007787 solid Substances 0.000 description 17
- 239000007788 liquid Substances 0.000 description 12
- 239000010985 leather Substances 0.000 description 11
- 229960002449 glycine Drugs 0.000 description 9
- 238000011081 inoculation Methods 0.000 description 9
- MEVQBEUFWYTMTA-UHFFFAOYSA-N 2-[(4,6-dichloro-1,3,5-triazin-2-yl)amino]-3-[2-[(4,6-dichloro-1,3,5-triazin-2-yl)oxy]phenyl]propanoic acid Chemical compound C1=CC=C(C(=C1)CC(C(=O)O)NC2=NC(=NC(=N2)Cl)Cl)OC3=NC(=NC(=N3)Cl)Cl MEVQBEUFWYTMTA-UHFFFAOYSA-N 0.000 description 8
- 229940077388 benzenesulfonate Drugs 0.000 description 7
- 229920001817 Agar Polymers 0.000 description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 6
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 6
- 239000008272 agar Substances 0.000 description 6
- XBDQKXXYIPTUBI-UHFFFAOYSA-N dimethylselenoniopropionate Natural products CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 description 6
- 239000008103 glucose Substances 0.000 description 6
- 238000011218 seed culture Methods 0.000 description 6
- 229910052708 sodium Inorganic materials 0.000 description 6
- 239000011734 sodium Substances 0.000 description 6
- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 description 5
- 241000193830 Bacillus <bacterium> Species 0.000 description 4
- 239000004471 Glycine Substances 0.000 description 3
- 235000019260 propionic acid Nutrition 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 2
- 229940037003 alum Drugs 0.000 description 1
- 125000003118 aryl group Chemical group 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000013905 glycine and its sodium salt Nutrition 0.000 description 1
- 239000004519 grease Substances 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000005445 natural material Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/22—Nature of the water, waste water, sewage or sludge to be treated from the processing of animals, e.g. poultry, fish, or parts thereof
- C02F2103/24—Nature of the water, waste water, sewage or sludge to be treated from the processing of animals, e.g. poultry, fish, or parts thereof from tanneries
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Water Supply & Treatment (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Environmental & Geological Engineering (AREA)
- Hydrology & Water Resources (AREA)
- Biodiversity & Conservation Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The invention discloses a compound microbial agent for treating COD pollutants in nonmetal organic synthetic tanning agent wastewater and application thereof, belonging to the technical field of microorganisms. The composite microbial agent is prepared by mixing bacillus pasteurianus, bacillus subtilis and bacillus badius, and the volume ratio of the composite microbial agent is (1-3): (1-3): (1-3), the compound microbial agent has specificity on the degradation of high-concentration organic pollutant COD by adjusting the formula of the fermentation culture medium of the bacillus pasteurianus, the bacillus subtilis and the bacillus badius. The invention also discloses application of the compound microbial agent in treating non-metal organic synthetic tanning agent wastewater containing triazine derivatives with 4, 6-dichloro-1, 3, 5-triazine as a main chain of aminoacetic acid groups, sodium benzenesulfonate groups or propionic acid groups, wherein when the compound microbial agent is added in a volume of 1% of the wastewater, the COD removal rates of high-concentration organic pollutants respectively reach 62.2%, 58.0% and 57.9%, and the compound microbial agent has obvious influence on water quality improvement.
Description
Technical Field
The invention belongs to the technical field of microorganisms, and particularly relates to a compound microbial agent for treating COD (chemical oxygen demand) pollutants in nonmetal organic synthetic tanning agent wastewater and application thereof.
Background
The traditional leather industry is one of the oldest industries, and as early as ancient times, humans tanned leather with natural substances such as grease, lipids and alum. With the progress of leather-making technology, leather is gradually tanned by various tanning agents. In the current leather production, the excellent leather-forming performance of chrome tanning plays a leading role in the leather production, but because a tanning agent used in the leather-making process can not be completely absorbed by leather, chrome is not completely absorbed, the chrome-containing waste liquid causes serious pollution to the environment, and simultaneously, due to the shortage of chrome resources, the domination of the chrome tanning method in the leather tanning is influenced. The search for new tanning agents and the exploration of new tanning methods and tanning processes are the necessary way for realizing sustainable development of the leather industry. Therefore, the development of chrome-free tanning technology and materials becomes a hot spot of current research. At present, the more researched chrome-free tanning method is the combined tanning method of a plant tanning agent and other organic tanning agents, and the combined tanning method can generate obvious synergistic effect and has higher shrinkage temperature of finished leather, thus becoming a standard tanning method.
In practical applications, the combination of tanning with certain chrome-free tanning agents can greatly improve the quality of finished leather in order to combine the advantages and disadvantages of each tanning agent. For example, A13+-Ti4+-Zr4+The polymetallic tanning can lead the Ts of finished leather to reach 100 ℃, but the polymetallic tanning has heavy metal pollution; the non-metal organic synthetic tanning agent belongs to a new generation of green chemicals, but the waste water of the non-metal organic synthetic tanning agent usually contains high concentration of organic pollutant COD. In order to solve the problem that the non-metal organic synthetic tanning agent contains high-concentration organic pollutant COD wastewater, the currently common treatment method is to treat the high-concentration organic pollutant COD by using a single microorganism, but the treatment effect is not ideal.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention aims to provide the composite microbial agent and the application thereof in treating high COD pollutants in the nonmetal organic synthetic tanning agent wastewater.
In order to achieve the purpose, the invention adopts the following technical scheme to realize the purpose:
the invention discloses a compound microbial agent for treating COD pollutants in nonmetal organic synthetic tanning agent wastewater, which is prepared by mixing bacillus pasteurii, bacillus subtilis and bacillus badius;
wherein the volume ratio of the pasteurella to the bacillus subtilis to the bacillus badius is (1-3) to (1-3).
Further, a fermentation medium for culturing the bacillus pasteurii comprises the following components in percentage by mass: 0.5 to 1.5 percent of oxazine derivative which takes 4, 6-dichloro-1, 3, 5-triazine as a main chain and contains aminoacetic acid group, 0.5 to 1.0 percent of ammonium chloride, 0.5 to 1.0 percent of sodium chloride and the balance of water; and the pH value of the fermentation medium is 7.0-7.4.
Further, a fermentation medium for culturing the bacillus subtilis comprises the following components in percentage by mass: 0.5 to 1.5 percent of oxazine derivative which takes 4, 6-dichloro-1, 3, 5-triazine as a main chain and contains sodium benzenesulfonate group, 0.5 to 1.0 percent of ammonium chloride, 0.5 to 1.0 percent of sodium chloride and the balance of water; and the pH value of the fermentation medium is 7.0-7.4.
Further, a fermentation medium for culturing the bacillus badius comprises the following components in percentage by mass: 0.5 to 1.5 percent of oxazine derivative which takes 4, 6-dichloro-1, 3, 5-triazine as a main chain and contains propionic acid group, 0.5 to 1.0 percent of ammonium chloride, 0.5 to 1.0 percent of sodium chloride and the balance of water; and the pH value of the fermentation medium is 7.0-7.4.
The invention also discloses application of the compound microbial agent for treating COD pollutants in the non-metal organic synthetic tanning agent wastewater in degradation of high-concentration COD in the non-metal organic synthetic tanning agent wastewater.
Further, the adding amount of the composite microbial agent for treating COD pollutants in the non-metal organic synthetic tanning agent wastewater is 1% of the volume of the non-metal organic synthetic tanning agent wastewater when the non-metal organic synthetic tanning agent wastewater is treated by the composite microbial agent.
Further, the removal rate of the complex microbial agent for treating the COD pollutant in the non-metallic organic syntan wastewater to the high concentration COD in the non-metallic organic syntan wastewater containing the oxazine derivative having 4, 6-dichloro-1, 3, 5-triazine as a main chain and containing an aminoacetic acid group was 62.2%.
Further, the removal rate of the compound microbial agent for treating COD pollutants in the non-metal organic synthetic tanning agent wastewater containing the oxazine derivative which takes 4, 6-dichloro-1, 3, 5-triazine as a main chain and contains sodium benzenesulfonate groups to the high-concentration COD in the non-metal organic synthetic tanning agent wastewater is 58.0%.
Further, the removal rate of the high concentration COD in the non-metallic organic syntan wastewater containing the oxazine derivative having 4, 6-dichloro-1, 3, 5-triazine as the main chain and containing an propionic acid group by the complex microbial agent for treating COD contaminants in the non-metallic organic syntan wastewater was 57.9%.
Compared with the prior art, the invention has the following beneficial effects:
the invention discloses a compound microbial agent for treating non-metal organic synthetic tanning agent COD-containing wastewater, which is prepared by mixing bacillus pasteurianus, bacillus subtilis and bacillus badius according to the volume ratio of (1-3): (1-3): (1-3), the mixture has good stability, and can degrade high-concentration pollutants COD in wastewater of the nonmetal organic synthetic tanning agent of the oxazine derivative which takes 4, 6-dichloro-1, 3, 5-triazine as a main chain and contains aminoacetic acid groups, the oxazine derivative which takes 4, 6-dichloro-1, 3, 5-triazine as a main chain and contains sodium benzenesulfonate groups and the oxazine derivative which takes 4, 6-dichloro-1, 3, 5-triazine as a main chain and contains propionic acid groups, and the removal rate reaches 62.2%, 58.0% and 57.9% respectively.
The reason why the complex microbial inoculant can achieve the removal efficiency is that the fermentation culture medium formulas of the pasteurella, the bacillus subtilis and the bacillus badius are innovatively adjusted during the treatment, and the triazine derivative which takes 4, 6-dichloro-1, 3, 5-triazine as a main chain and contains aminoacetic acid groups, the triazine derivative which takes 4, 6-dichloro-1, 3, 5-triazine as a main chain and contains sodium benzenesulfonate groups and the non-metal organic synthetic tanning agent which takes 4, 6-dichloro-1, 3, 5-triazine as a main chain and contains propionic acid groups are respectively introduced into the culture medium of the strain, so that the compound microbial agent has specificity on the degradation of high-concentration organic pollutants COD, therefore, the method has important application prospect in solving the current situation that the wastewater of the non-metal organic synthetic tanning agent contains high-concentration organic pollutant COD.
The invention also discloses application of the microbial composite microbial inoculum for treating the high-COD wastewater of the non-metal organic synthetic tanning agent in degradation of high-concentration organic pollutant COD.
Furthermore, the microbial composite inoculant can be used after being mixed with non-metal organic synthetic tanning agent wastewater containing high-concentration organic pollutant COD according to the volume ratio of 1%.
Drawings
FIG. 1 is a graph showing the degradation rate of the complex microbial inoculant of example 1 of the present invention to high concentration organic pollutant COD in non-metal organic synthetic tanning agent wastewater containing 4, 6-dichloro-1, 3, 5-triazine-2-amino acetic acid;
FIG. 2 is a graph showing the degradation rate of the complex microbial inoculant of example 2 of the present invention to high concentration organic pollutant COD in wastewater of non-metal organic synthetic tanning agent containing sodium- [4, 6-dichloro-1, 3, 5-triazine-2-amino ] -benzenesulfonate;
FIG. 3 is a graph showing the degradation rate of the complex microbial inoculant of example 3 of the present invention to high-concentration organic pollutant COD in the wastewater of non-metal organic synthetic tanning agent containing 2- ((4, 6-dichloro-1, 3, 5-triazin-2-yl) amino) 3- (2- ((4, 6-dichloro-1, 3, 5-triazin-2-yl) oxy) phenyl) propionic acid.
Detailed Description
In order to make the technical solutions of the present invention better understood, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
It should be noted that the terms "first," "second," and the like in the description and claims of the present invention and in the drawings described above are used for distinguishing between similar elements and not necessarily for describing a particular sequential or chronological order. It is to be understood that the data so used is interchangeable under appropriate circumstances such that the embodiments of the invention described herein are capable of operation in sequences other than those illustrated or described herein. Furthermore, the terms "comprises," "comprising," and "having," and any variations thereof, are intended to cover a non-exclusive inclusion, such that a process, method, system, article, or apparatus that comprises a list of steps or elements is not necessarily limited to those steps or elements expressly listed, but may include other steps or elements not expressly listed or inherent to such process, method, article, or apparatus.
The invention is described in further detail below with reference to the accompanying drawings:
example 1
A composite microbial agent for treating high-concentration organic pollutant COD wastewater in wastewater of non-metal organic synthetic tanning agent containing 4, 6-dichloro-1, 3, 5-triazine-2-glycine (aminoacetic acid-based organic synthetic compound) is prepared by the following method:
1) solid slant culture: respectively inoculating the pasteurella, the bacillus subtilis and the bacillus badius into a beef extract peptone culture medium, a bacillus subtilis solid culture medium and a beef extract peptone culture medium, and culturing at constant temperature of 30 ℃ for 2d to fully activate the strains; the solid culture medium is as follows: the beef extract peptone solid medium formula is as follows: 0.5 wt% of beef extract, 1.0 wt% of peptone, 0.5 wt% of sodium chloride, 2 wt% of agar and the balance of water, wherein the pH value is 7.0-7.4; the bacillus subtilis solid culture medium comprises the following components in percentage by weight: 2 wt% of glucose, 1.5 wt% of peptone, 0.5 wt% of sodium chloride, 0.05 wt% of beef extract, 2 wt% of agar and the balance of water, wherein the pH value is 7.0-7.4.
2) First-order seed culture: respectively inoculating the activated bacillus pasteurii, bacillus subtilis and bacillus badius in the step 1) into a beef extract peptone liquid culture medium, a bacillus subtilis liquid culture medium and a beef extract peptone liquid culture medium, and culturing for 2d at the temperature of 30 ℃ and the speed of 130r/min when OD is reached600Stopping culturing when the value reaches 0.8, and respectively obtaining first-stage seed solutions; the beef extract peptone liquid culture medium comprises the following components in parts by weight: 0.5 wt% of beef extract, 1.0 wt% of peptone, 0.5 wt% of sodium chloride and the balance of water, wherein the pH value is 7.0-7.4; the bacillus subtilis solid culture medium comprises the following components in percentage by weight: 2 wt% of glucose, 1.5 wt% of peptone, 0.5 wt% of sodium chloride, 0.05 wt% of beef extract and the balance of water, wherein the pH value is 7.0-7.4.
3) Secondary seed culture: respectively inoculating the primary seed liquid of the bacillus pasteurianus, the bacillus subtilis and the bacillus badius obtained in the step 2) into a beef extract peptone liquid culture medium, a bacillus subtilis liquid culture medium and a beef extract peptone liquid culture medium, and culturing for 2d at 30 ℃ and 130r/min to respectively obtain secondary seed liquid; the formula of the culture medium is the same as that in step 2).
Inoculating the secondary seed liquid of the bacillus pasteurii obtained in the step 3) into a bacillus pasteurii fermentation culture medium in an inoculation amount of 3% (volume ratio), and performing fermentation culture at 30 ℃ and 120r/min for 7d to obtain a microbial inoculum A; the fermentation medium of the pasteurella comprises: 0.5 wt% of 4, 6-dichloro-1, 3, 5-triazine-2-amino acetic acid, 1.0 wt% of ammonium chloride, 0.5 wt% of sodium chloride and the balance of water, wherein the pH value is 7.0;
inoculating the bacillus subtilis secondary seed solution obtained in the step 3) into a bacillus subtilis fermentation culture medium in an inoculation amount of 3% (volume ratio), and performing fermentation culture at 30 ℃ and 130r/min for 7d to obtain a microbial inoculum B; the bacillus subtilis fermentation medium comprises: 0.5 wt% of sodium p- [4, 6-dichloro-1, 3, 5-triazine-2-amino ] -benzenesulfonate, 1.0 wt% of ammonium chloride, 0.5 wt% of sodium chloride and the balance of water, wherein the pH is 7.0;
inoculating the bacillus badius secondary seed liquid obtained in the step 3) into a bacillus badius fermentation medium in an inoculation amount of 3% (volume ratio), and performing fermentation culture at 30 ℃ and 140r/min for 7d to obtain a microbial inoculum C; the bacillus badius fermentation medium is as follows: contains 0.5 wt% of 2- ((4, 6-dichloro-1, 3, 5-triazin-2-yl) amino) 3- (2- ((4, 6-dichloro-1, 3, 5-triazin-2-yl) oxy) phenyl) propionic acid, 1.0 wt% of ammonium chloride, 0.5 wt% of sodium chloride and the balance of water, and the pH is 7.0;
and mixing the microbial inoculum A, the microbial inoculum B and the microbial inoculum C according to the volume ratio of 1:1:1 to obtain the compound microbial inoculum.
The composite microbial inoculum prepared in the example is used for treating wastewater containing high concentration organic pollutant COD of the non-metal organic synthetic tanning agent containing 4, 6-dichloro-1, 3, 5-triazine-2-glycine, and is added into wastewater containing high concentration organic pollutant COD of the non-metal organic synthetic tanning agent containing 4, 6-dichloro-1, 3, 5-triazine-2-glycine, wherein the adding proportion of the composite microbial inoculum is 1% of the volume of the wastewater.
Referring to fig. 1, the degradation rate of high concentration organic pollutant COD in the nonmetal organic synthetic tanning agent wastewater containing 4, 6-dichloro-1, 3, 5-triazine-2-amino acetic acid reaches 62.2%.
Example 2
A composite microbial agent for treating high-concentration organic pollutant COD wastewater containing non-metal organic synthetic tanning agent of p- [4, 6-dichloro-1, 3, 5-triazine-2-amino ] -benzene sulfonic acid sodium (aryl organic synthetic compound) is prepared by the following method:
1) solid slant culture: respectively inoculating the pasteurella, the bacillus subtilis and the bacillus badius into a beef extract peptone culture medium, a bacillus subtilis solid culture medium and a beef extract peptone culture medium, and culturing at the constant temperature of 33 ℃ for 2d to fully activate the strains; the solid culture medium is as follows: the beef extract peptone solid medium formula is as follows: 0.5 wt% of beef extract, 1.0 wt% of peptone, 0.5 wt% of sodium chloride, 2 wt% of agar and the balance of water, wherein the pH value is 7.0-7.4; the bacillus subtilis solid culture medium comprises the following components in percentage by weight: 2 wt% of glucose, 1.5 wt% of peptone, 0.5 wt% of sodium chloride, 0.05 wt% of beef extract, 2 wt% of agar and the balance of water, wherein the pH value is 7.0-7.4.
2) First-order seed culture: mixing the activated pasteurella and hay in step 1)Respectively inoculating Bacillus and Bacillus badius into beef extract peptone liquid culture medium, Bacillus subtilis liquid culture medium, and beef extract peptone liquid culture medium, culturing at 33 deg.C and 140r/min for 2d, and culturing at OD600Stopping culturing when the value reaches 0.8, and respectively obtaining first-stage seed solutions; the beef extract peptone liquid culture medium comprises the following components in parts by weight: 0.5 wt% of beef extract, 1.0 wt% of peptone, 0.5 wt% of sodium chloride and the balance of water, wherein the pH value is 7.0-7.4; the bacillus subtilis solid culture medium comprises the following components in percentage by weight: 2 wt% of glucose, 1.5 wt% of peptone, 0.5 wt% of sodium chloride, 0.05 wt% of beef extract and the balance of water, wherein the pH value is 7.0-7.4.
3) Secondary seed culture: respectively inoculating the primary seed liquid of the bacillus pasteurianus, the bacillus subtilis and the bacillus badius obtained in the step 2) into a beef extract peptone liquid culture medium, a bacillus subtilis liquid culture medium and a beef extract peptone liquid culture medium, and culturing for 2d at 33 ℃ and 140r/min to respectively obtain secondary seed liquid; the formula of the culture medium is the same as that in step 2).
Inoculating the bacillus secondary seed solution obtained in the step 3) into a bacillus pasteurii fermentation culture medium in an inoculation amount of 10% (volume ratio), and performing fermentation culture at 33 ℃ and 130r/min for 7d to obtain a microbial inoculum A; the fermentation medium of the pasteurella comprises: 0.5 wt% of 4, 6-dichloro-1, 3, 5-triazine-2-amino acetic acid, 1.0 wt% of ammonium chloride, 0.5 wt% of sodium chloride and the balance of water, wherein the pH value is 7.2;
inoculating the bacillus subtilis secondary seed solution obtained in the step 3) into a bacillus subtilis fermentation culture medium in an inoculation amount of 10% (volume ratio), and performing fermentation culture at 33 ℃ and 140r/min for 7d to obtain a microbial inoculum B; the bacillus subtilis fermentation medium comprises: 0.5 wt% of sodium p- [4, 6-dichloro-1, 3, 5-triazine-2-amino ] -benzenesulfonate, 1.0 wt% of ammonium chloride, 0.5 wt% of sodium chloride and the balance of water, wherein the pH is 7.2;
inoculating the bacillus badius secondary seed liquid obtained in the step 3) into a bacillus badius fermentation medium in an inoculation amount of 10% (volume ratio), and performing fermentation culture at 33 ℃ and 150r/min for 7d to obtain a microbial inoculum C; the bacillus badius fermentation medium is as follows: contains 0.5 wt% of 2- ((4, 6-dichloro-1, 3, 5-triazin-2-yl) amino) 3- (2- ((4, 6-dichloro-1, 3, 5-triazin-2-yl) oxy) phenyl) propionic acid, 1.0 wt% of ammonium chloride, 0.5 wt% of sodium chloride and the balance of water, and the pH is 7.2;
and mixing the microbial inoculum A, the microbial inoculum B and the microbial inoculum C according to the volume ratio of 1:3:2 to obtain the compound microbial inoculum.
The composite microbial inoculum prepared in the embodiment is used for treating wastewater containing high-concentration organic pollutant COD of the nonmetal organic synthetic tanning agent containing sodium p- [4, 6-dichloro-1, 3, 5-triazine-2-amino ] -benzene sulfonate, and the composite microbial inoculum prepared in the embodiment is added into the wastewater containing high-concentration organic pollutant COD of the nonmetal organic synthetic tanning agent containing sodium p- [4, 6-dichloro-1, 3, 5-triazine-2-amino ] -benzene sulfonate, wherein the adding proportion of the composite microbial inoculum is 1% of the volume of the wastewater.
Referring to fig. 2, the degradation rate of high concentration organic pollutant COD in the non-metal organic synthetic tanning agent wastewater containing sodium p- [4, 6-dichloro-1, 3, 5-triazine-2-amino ] -benzene sulfonate reaches 58.0%.
Example 3
A composite microbial agent for treating high-concentration organic pollutant COD wastewater in wastewater of non-metal organic synthetic tanning agent containing 2- ((4, 6-dichloro-1, 3, 5-triazine-2-yl) amino) 3- (2- ((4, 6-dichloro-1, 3, 5-triazine-2-yl) oxy) phenyl) propionic acid (propionic acid-based organic synthetic compound) is prepared by the following method:
1) solid slant culture: respectively inoculating the pasteurella, the bacillus subtilis and the bacillus badius into a beef extract peptone culture medium, a bacillus subtilis solid culture medium and a beef extract peptone culture medium, and culturing at the constant temperature of 37 ℃ for 2d to fully activate the strains; the solid culture medium is as follows: the beef extract peptone solid medium formula is as follows: 0.5 wt% of beef extract, 1.0 wt% of peptone, 0.5 wt% of sodium chloride, 2 wt% of agar and the balance of water, wherein the pH value is 7.0-7.4; the bacillus subtilis solid culture medium comprises the following components in percentage by weight: 2 wt% of glucose, 1.5 wt% of peptone, 0.5 wt% of sodium chloride, 0.05 wt% of beef extract, 2 wt% of agar and the balance of water, wherein the pH value is 7.0-7.4.
2) First-order seed culture: the activated pasteurella and bacillus subtilis in the step 1) are treatedRespectively inoculating Bacillus badius and Bacillus badius to beef extract peptone liquid culture medium, Bacillus subtilis liquid culture medium, and beef extract peptone liquid culture medium, culturing at 37 deg.C and 150r/min for 2d, and culturing at OD600Stopping culturing when the value reaches 0.8, and respectively obtaining first-stage seed solutions; the beef extract peptone liquid culture medium comprises the following components in parts by weight: 0.5 wt% of beef extract, 1.0 wt% of peptone, 0.5 wt% of sodium chloride and the balance of water, wherein the pH value is 7.0-7.4; the bacillus subtilis liquid culture medium comprises the following components in percentage by weight: 2 wt% of glucose, 1.5 wt% of peptone, 0.5 wt% of sodium chloride, 0.05 wt% of beef extract and the balance of water, wherein the pH value is 7.0-7.4.
3) Secondary seed culture: respectively inoculating the primary seed liquid of the bacillus pasteurianus, the bacillus subtilis and the bacillus badius obtained in the step 2) into a beef extract peptone liquid culture medium, a bacillus subtilis liquid culture medium and a beef extract peptone liquid culture medium, and culturing for 2d at 37 ℃ and 150r/min to respectively obtain secondary seed liquid; the formula of the culture medium is the same as that in step 2).
Inoculating the secondary seed liquid of the bacillus pasteurii obtained in the step 3) into a bacillus pasteurii fermentation culture medium in an inoculation amount of 15% (volume ratio), and performing fermentation culture at 37 ℃ and 140r/min for 7d to obtain a microbial inoculum A; the fermentation medium of the pasteurella comprises: 0.5 wt% of 4, 6-dichloro-1, 3, 5-triazine-2-amino acetic acid, 1.0 wt% of ammonium chloride, 0.5 wt% of sodium chloride and the balance of water, wherein the pH value is 7.4;
inoculating the bacillus subtilis secondary seed solution obtained in the step 3) into a bacillus subtilis fermentation culture medium in an inoculation amount of 15% (volume ratio), and performing fermentation culture at 37 ℃ and 150r/min for 7d to obtain a microbial inoculum B; the bacillus subtilis fermentation medium comprises: 0.5 wt% of sodium p- [4, 6-dichloro-1, 3, 5-triazine-2-amino ] -benzenesulfonate, 1.0 wt% of ammonium chloride, 0.5 wt% of sodium chloride and the balance of water, wherein the pH is 7.4;
inoculating the bacillus badius secondary seed liquid obtained in the step 3) into a bacillus badius fermentation medium in an inoculation amount of 15% (volume ratio), and performing fermentation culture at 37 ℃ and 160r/min for 7d to obtain a microbial inoculum C; the bacillus badius fermentation medium is as follows: 0.5 wt% of 2- ((4, 6-dichloro-1, 3, 5-triazin-2-yl) amino) 3- (2- ((4, 6-dichloro-1, 3, 5-triazin-2-yl) oxy) phenyl) propanoic acid, 1.0 wt% of ammonium chloride, 0.5 wt% of sodium chloride, and the balance of water, and the pH is 7.4;
and mixing the microbial inoculum A, the microbial inoculum B and the microbial inoculum C according to the volume ratio of 1:2:3 to obtain the compound microbial inoculum.
The non-metallic organic synthetic tanning agent containing 2- ((4, 6-dichloro-1, 3, 5-triazine-2-yl) amino) 3- (2- ((4, 6-dichloro-1, 3, 5-triazine-2-yl) oxy) phenyl) propionic acid containing high concentration organic pollutant COD waste water is treated by the compound microbial agent prepared in the embodiment, the compound microbial agent is added into the non-metallic organic synthetic tanning agent containing 2- ((4, 6-dichloro-1, 3, 5-triazine-2-yl) amino) 3- (2- ((4, 6-dichloro-1, 3, 5-triazine-2-yl) oxy) phenyl) propionic acid containing high concentration organic pollutant COD waste water, the adding proportion of the compound microbial agent is 1 percent of the volume of the wastewater.
Referring to fig. 3, in the non-metal organic synthetic tanning agent wastewater containing 2- ((4, 6-dichloro-1, 3, 5-triazin-2-yl) amino) 3- (2- ((4, 6-dichloro-1, 3, 5-triazin-2-yl) oxy) phenyl) propionic acid, the degradation rate of high-concentration organic pollutant COD reaches 57.9%.
In summary, the composite microbial agent disclosed by the invention comprises bacillus pasteurii, bacillus subtilis and bacillus auburn mixed, wherein the volume ratio of the bacillus pasteurii to the bacillus subtilis to the bacillus auburn is (1-3): (1-3): (1-3). The invention also discloses a preparation method of the microbial inoculum of the composite microorganism and application of the microbial inoculum in degrading high-concentration organic pollutants COD in a non-metal organic synthetic tanning agent (containing oxazine derivatives which take 4, 6-dichloro-1, 3, 5-triazine as a main chain and contain aminoacetic acid groups, oxazine derivatives which take 4, 6-dichloro-1, 3, 5-triazine as a main chain and contain sodium benzenesulfonate groups and oxazine derivatives which take 4, 6-dichloro-1, 3, 5-triazine as a main chain and contain propionic acid groups). The microbial composite microbial inoculum is used for treating high-concentration organic pollutant COD, is easy to culture, is simple to operate and low in price, and can degrade high-concentration COD in wastewater of non-metal organic synthetic tanning agents (containing oxazine derivatives which take 4, 6-dichloro-1, 3, 5-triazine as a main chain and contain aminoacetic acid groups, oxazine derivatives which take 4, 6-dichloro-1, 3, 5-triazine as a main chain and contain sodium benzenesulfonate groups and oxazine derivatives which take 4, 6-dichloro-1, 3, 5-triazine as a main chain and contain propionic acid groups), and the removal rates of the COD in the wastewater respectively reach 62.2%, 58.0% and 57.9%.
The above-mentioned contents are only for illustrating the technical idea of the present invention, and the protection scope of the present invention is not limited thereby, and any modification made on the basis of the technical idea of the present invention falls within the protection scope of the claims of the present invention.
Claims (9)
1. A compound microbial agent for treating COD pollutants in non-metal organic synthetic tanning agent wastewater is characterized in that the compound microbial agent is prepared by mixing bacillus pasteurii, bacillus subtilis and bacillus badius;
wherein the volume ratio of the pasteurella to the bacillus subtilis to the bacillus badius is (1-3) to (1-3).
2. The complex microbial inoculant for treating COD (chemical oxygen demand) pollutants in non-metallic organic syntan wastewater of claim 1, wherein a fermentation medium for culturing the Bacillus pasteurianus comprises the following components in percentage by mass: 0.5 to 1.5 percent of oxazine derivative which takes 4, 6-dichloro-1, 3, 5-triazine as a main chain and contains aminoacetic acid group, 0.5 to 1.0 percent of ammonium chloride, 0.5 to 1.0 percent of sodium chloride and the balance of water; and the pH value of the fermentation medium is 7.0-7.4.
3. The compound microbial inoculant for treating COD (chemical oxygen demand) pollutants in non-metallic organic syntan wastewater of claim 1, wherein a fermentation medium for culturing the bacillus subtilis comprises the following components in percentage by mass: 0.5 to 1.5 percent of oxazine derivative which takes 4, 6-dichloro-1, 3, 5-triazine as a main chain and contains sodium benzenesulfonate group, 0.5 to 1.0 percent of ammonium chloride, 0.5 to 1.0 percent of sodium chloride and the balance of water; and the pH value of the fermentation medium is 7.0-7.4.
4. The compound microbial inoculant for treating COD (chemical oxygen demand) pollutants in non-metallic organic synthetic tanning agent wastewater according to claim 1, wherein a fermentation medium for culturing the Bacillus badius comprises the following components in percentage by mass: 0.5 to 1.5 percent of oxazine derivative which takes 4, 6-dichloro-1, 3, 5-triazine as a main chain and contains propionic acid group, 0.5 to 1.0 percent of ammonium chloride, 0.5 to 1.0 percent of sodium chloride and the balance of water; and the pH value of the fermentation medium is 7.0-7.4.
5. The use of the compound microbial inoculant for treating COD contaminants in non-metallic organic syntan wastewater as defined in any one of claims 1 to 4 in the degradation of high concentration COD in non-metallic organic syntan wastewater.
6. The use according to claim 5, wherein the complex microbial inoculant for treating COD contaminants in the non-metallic organic syntan wastewater is added in an amount of 1% by volume of the non-metallic organic syntan wastewater.
7. The use according to claim 5, wherein the complex microbial agent for treating COD contaminants in non-metallic organic syntan wastewater has a removal rate of 62.2% for high concentration COD in non-metallic organic syntan wastewater containing oxazine derivatives having a backbone of 4, 6-dichloro-1, 3, 5-triazine and containing aminoacetic acid groups.
8. The use according to claim 5, wherein the removal rate of the complex microbial agent for treating COD contaminants in the non-metallic organic syntan wastewater containing oxazine derivatives having a main chain of 4, 6-dichloro-1, 3, 5-triazine and containing sodium benzenesulfonate groups by the high concentration COD in the non-metallic organic syntan wastewater is 58.0%.
9. The use according to claim 5, wherein the complex microbial inoculant used for treating COD contaminants in non-metallic organic syntan wastewater has a removal rate of 57.9% for high concentration COD in non-metallic organic syntan wastewater containing oxazine derivatives having a backbone chain of 4, 6-dichloro-1, 3, 5-triazine and containing propionic acid groups.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010137051.4A CN111235062B (en) | 2020-03-02 | 2020-03-02 | Compound microbial agent for treating COD (chemical oxygen demand) pollutants in nonmetal organic synthetic tanning agent wastewater and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010137051.4A CN111235062B (en) | 2020-03-02 | 2020-03-02 | Compound microbial agent for treating COD (chemical oxygen demand) pollutants in nonmetal organic synthetic tanning agent wastewater and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN111235062A CN111235062A (en) | 2020-06-05 |
| CN111235062B true CN111235062B (en) | 2021-07-27 |
Family
ID=70875179
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010137051.4A Active CN111235062B (en) | 2020-03-02 | 2020-03-02 | Compound microbial agent for treating COD (chemical oxygen demand) pollutants in nonmetal organic synthetic tanning agent wastewater and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111235062B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112481151A (en) * | 2020-10-14 | 2021-03-12 | 耿澄环保科技(上海)有限公司 | Biological bacterium agent for treating paint debonding wastewater and debonding treatment process thereof |
| CN115851532A (en) * | 2022-12-05 | 2023-03-28 | 中国科学院天津工业生物技术研究所 | Compound microbial agent and application thereof in leather wastewater treatment |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105400722A (en) * | 2015-12-11 | 2016-03-16 | 北京沃土天地生物科技股份有限公司 | Composite microbial agent, and preparation method and application thereof |
| CN106047772A (en) * | 2016-08-09 | 2016-10-26 | 湖北凌卓生物工程有限公司 | Composite microbial agent for treating high-ammonia-nitrogen sewage and preparation method and application thereof |
-
2020
- 2020-03-02 CN CN202010137051.4A patent/CN111235062B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105400722A (en) * | 2015-12-11 | 2016-03-16 | 北京沃土天地生物科技股份有限公司 | Composite microbial agent, and preparation method and application thereof |
| CN106047772A (en) * | 2016-08-09 | 2016-10-26 | 湖北凌卓生物工程有限公司 | Composite microbial agent for treating high-ammonia-nitrogen sewage and preparation method and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| ２株枯草芽孢杆菌的分离鉴定及特性研究;巴翠玉等;《华南农业大学学报》;20171231;第38卷(第3期);第46-51页 * |
| Aerobic nitrification-denitrification by heterotrophic Bacillus strains;JOONG,K.K.等;《Bioresour. Technol.》;20051231;第96卷(第17期);第1897-1906页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN111235062A (en) | 2020-06-05 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US11168012B2 (en) | Method for realizing rapid startup of denitrification biofilter | |
| CN111235062B (en) | Compound microbial agent for treating COD (chemical oxygen demand) pollutants in nonmetal organic synthetic tanning agent wastewater and application thereof | |
| CN109626599B (en) | Composite microbial inoculum for enhancing denitrification effect of low-temperature low-carbon-nitrogen-ratio sewage and preparation method and application thereof | |
| Salimi et al. | Characterizing metabolic interactions in a clostridial co-culture for consolidated bioprocessing | |
| CN108866115B (en) | Method for efficiently producing volatile fatty acid by using vinasse and sludge in combined anaerobic fermentation manner | |
| CN106434469B (en) | Low-temperature-resistant nitrifying bacteria agent and preparation method and application thereof | |
| WO2021077453A1 (en) | Pseudomonas stutzeri, composite microbial inoculum prepared using the pseudomonas stutzeri, and use of the composite microbial inoculum | |
| CN111252902A (en) | Process method for treating dehydroacetic acid mixed wastewater and application | |
| CN104762291A (en) | Preparation method for immobilization particle used for reducing activated sludge | |
| EP0604708A1 (en) | Fermentation method for the fast production of methane | |
| CN103373764A (en) | Method for quickly starting short-cut nitrification and denitrification of ammonia-containing wastewater | |
| CN106746156B (en) | Method for treating epichlorohydrin production wastewater | |
| 吕永康 et al. | Isolation and characterization of heterotrophic nitrifying strain W1 | |
| CN105948243A (en) | Method for rapidly culturing anaerobic granular sludge applicable to pharmaceutical wastewater treatment | |
| CN111139198A (en) | Lactobacillus parvum GBW-HB1903 and application thereof | |
| CN114620900B (en) | Denitrification treatment method for kitchen anaerobic wastewater | |
| CN110305914A (en) | A kind of method that reinforcement sludge produces sour mixed fermentation | |
| CN109593791A (en) | A method of improving excess sludge anaerobic fermentation hydrogen output | |
| CN107325982B (en) | Process for advanced treatment of wastewater from production of hydroxymethylcellulose by using microbial preparation | |
| CN109761461B (en) | Method for treating excess sludge by using microorganisms | |
| CN109775919B (en) | Biochemical environment-friendly process for effectively treating xanthan gum industrial wastewater | |
| CN111099722B (en) | Composition for promoting denitrification and application thereof | |
| CN112592853A (en) | Microbial agent containing alcaligenes faecalis and application thereof | |
| CN113151141B (en) | Culture method of strain for sewage treatment and culture medium thereof | |
| CN111763645A (en) | Combined fermentation method of nitrifying-denitrifying bacteria composite microecological preparation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20231120 Address after: 710000, Room B-48, 5th Floor, Building 1, Amazon AWS Joint Innovation Center, 3639 Eurasia Avenue, Chanba Ecological Zone, Xi'an City, Shaanxi Province [Cluster] Patentee after: Xi'an Xiuchuan Environmental Protection Technology Co.,Ltd. Address before: 710021 Shaanxi province Xi'an Weiyang University Park Patentee before: SHAANXI University OF SCIENCE & TECHNOLOGY |