CN111227988A - Brain stereotactic injection device - Google Patents
Brain stereotactic injection device Download PDFInfo
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- CN111227988A CN111227988A CN202010187121.7A CN202010187121A CN111227988A CN 111227988 A CN111227988 A CN 111227988A CN 202010187121 A CN202010187121 A CN 202010187121A CN 111227988 A CN111227988 A CN 111227988A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D7/00—Devices or methods for introducing solid, liquid, or gaseous remedies or other materials into or onto the bodies of animals
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D3/00—Appliances for supporting or fettering animals for operative purposes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M5/00—Devices for bringing media into the body in a subcutaneous, intra-vascular or intramuscular way; Accessories therefor, e.g. filling or cleaning devices, arm-rests
- A61M5/008—Racks for supporting syringes or needles
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M5/00—Devices for bringing media into the body in a subcutaneous, intra-vascular or intramuscular way; Accessories therefor, e.g. filling or cleaning devices, arm-rests
- A61M5/46—Devices for bringing media into the body in a subcutaneous, intra-vascular or intramuscular way; Accessories therefor, e.g. filling or cleaning devices, arm-rests having means for controlling depth of insertion
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D3/00—Appliances for supporting or fettering animals for operative purposes
- A61D2003/003—Appliances for supporting or fettering animals for operative purposes with head or neck restraining means
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Abstract
The invention discloses a brain stereotactic injection device which comprises a shell, a positioning component, a fixing component, an injection component and a leveling component, wherein the shell defines a containing cavity for placing an animal to be tested, the shell is provided with upper and lower scales which extend along the vertical direction, the positioning component is slidably arranged on the shell, the positioning component is provided with a first scale and a second scale, the positioning component is provided with a matching hole, the fixing component is arranged in the containing cavity and used for fixing the animal to be tested in the containing cavity, the injection component can penetrate through the matching hole and extend into the containing cavity, the injection component is provided with a pointer pointing to the upper and lower scales, the injection component is used for injecting an experimental reagent to the animal to be tested, the leveling component can penetrate through the matching hole and extend into the containing cavity, and the leveling component is used for adjusting the head of the animal to be tested. The brain stereotactic injection device has simple structure and convenient use.
Description
Technical Field
The invention relates to the technical field of biological experiment articles, in particular to a brain stereotactic injection device.
Background
In the field of brain science basic research, intracerebral injection is an important method for animal experiments, and plays an important role in establishing disease models, intracerebral administration treatment, exploring projection relations among central nuclei and the like. Injection samples such as viruses, cells, protein molecules, drugs, labeled dye probes and the like are injected into the brain and can directly act on a target brain area of a living animal, including neurons, glial cells, blood vessels, microenvironment in the brain area and the like, and the accurate brain area positioning administration does not relate to the drug metabolism of organ tissues and the blood brain barrier, namely, the accurate brain area positioning administration can accurately reflect the direct action of the drugs compared with results obtained by other administration routes.
The operation method of the conventional commercialized stereotactic injection device in the laboratory needs the combination of a laboratory table, a brain stereotactic instrument, a micro-injection pump and a skull drill. However, the method has the disadvantages that the instrument needs a fixed experiment table and a stable facility as supports, the equipment cost is high, the operation method is complicated, the experiment time is long, the time from fixing the mouse to finishing injection is 40-60 minutes, the laboratory mouse is easily damaged and even died by the operation of a novice hand, and the accurate and rapid administration in the brain area under the condition of limited experiment space and equipment cannot be met. The position distribution of each nucleus group in the brain is complicated, and the injection needle can not be used for direct intracranial administration and can not accurately inject a sample into a target brain area, so a set of convenient and practical intracerebral stereotaxic injection tool is needed.
Disclosure of Invention
The invention aims to provide a brain stereotactic injection device which is simple in structure and convenient to use.
In order to achieve the technical effects, the technical scheme of the invention is as follows:
the invention discloses a brain stereotactic injection device, comprising: the device comprises a shell, a positioning device and a control device, wherein the shell defines an accommodating cavity for accommodating an animal to be tested, and the shell is provided with upper and lower scales extending in the vertical direction; the positioning assembly is slidably arranged on the shell, a first scale and a second scale are arranged on the positioning assembly, the extending direction of the first scale is perpendicular to the extending direction of the second scale, and a matching hole is formed in the positioning assembly; the fixing component is arranged in the accommodating cavity and is used for fixing the animal to be tested in the accommodating cavity; the injection assembly can penetrate through the matching hole and extend into the accommodating cavity, a pointer pointing to the upper scale and the lower scale is arranged on the injection assembly, and the injection assembly is used for injecting an experimental reagent to the animal to be tested; the leveling assembly can pass through the matching hole and stretch into the accommodating cavity and is used for adjusting the head of the animal to be tested.
In some embodiments, the positioning assembly comprises: the first scale is provided with the first scale; the second scale is connected with the first scale, the second scale is provided with second scales, and the matching hole is formed in the connecting end of the first scale and the second scale; the slider, the slider is two, two the slider overlaps respectively to be established first scale with on the second scale, be equipped with on the casing with slider complex spout.
In some embodiments, the fixation assembly comprises a head fixation module comprising: the upright post is connected to the bottom wall of the shell; a support for supporting the mandible of the animal to be tested; and one end of the rotating shaft is connected with the supporting piece, and the other end of the rotating shaft is connected with the upright post through threads.
In some embodiments, the fixing component further includes two ear rod modules, two ear rod modules are respectively arranged on two oppositely arranged side walls of the shell in a penetrating manner, and two ear rod modules are used for stopping against the lower edge bone socket outside the ear canal of the animal to be tested.
In some specific embodiments, each of the ear stem modules comprises: the ear fixing piece is arranged on the side wall of the shell in a penetrating way, and ear fixing scales are arranged on the ear fixing piece; an adjustment component that cooperates with the ear mount to adjust a height position of the ear mount; a retaining member that fits over the adjustment member, the retaining member being capable of stopping against the adjustment member to lock the ear mount.
In some more specific embodiments, the adjustment member comprises: the adjusting sleeve is matched on the shell, an adjusting groove is formed in the upper end of the adjusting sleeve, an adjusting threaded hole is formed in the lower end of the adjusting sleeve, and the adjusting groove is communicated with the adjusting threaded hole; the adjusting block is slidably arranged in the adjusting groove along the vertical direction and is sleeved on the ear fixing piece; and the adjusting stud is matched in the adjusting threaded hole, and the upper end of the adjusting stud is stopped against the adjusting block.
In some embodiments, the injection assembly comprises: an injection syringe; the needle cylinder is matched in the connecting hole, the pointer is arranged at the other end of the connecting rod, and the connecting rod is a telescopic rod; a locking piece, the locking piece is matched on the connecting rod, and the locking piece is used for locking the injection syringe on the connecting rod.
In some specific embodiments, the leveling assembly comprises: the leveling device comprises a leveling main body, a leveling mechanism and a leveling mechanism, wherein a leveling groove is arranged on the leveling main body; the level gauge is matched in the leveling groove; the leveling device comprises two branching pieces, wherein the two branching pieces are rotatably arranged below the leveling main body and can be stopped against the skull of the animal to be tested.
In some more specific embodiments, each of the bifurcations includes: the matching part is connected with the leveling assembly through a pin shaft; the forked portion is connected with the matching portion, and one end, far away from the matching portion, of the forked portion is formed into a conical tip shape.
In some more specific embodiments, the leveling body comprises: the leveling rod can be matched in the matching hole, and the forked piece is matched at the lower end of the leveling rod; the leveling block is matched with the upper end of the leveling rod, and the leveling groove is formed in the leveling block.
The brain stereotactic injection device of this embodiment, owing to be equipped with fixed subassembly and the leveling subassembly of fixing and adjusting the animal gesture of waiting to experiment, and have the locating component who is used for the location injection subassembly and fix a position the upper and lower scale and the pointer of injection depth, simplified the operation process of stereotactic injection, realized standardized, the stereotactic injection operation of accuracy, saved experimental space, promoted experimental efficiency. In addition, the whole brain stereotactic injection device has very simple structure and small volume, and better solves the problems of high instrument cost, complex operation and the like in the existing stereotactic injection experiment.
Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention.
Drawings
Fig. 1 is a partial structural schematic diagram of a brain stereotactic injection apparatus according to an embodiment of the present invention.
Fig. 2 is a schematic structural diagram of a leveling component of a brain stereotactic injection apparatus of an embodiment of the present invention fitted in a fitting hole.
Fig. 3 is a schematic structural diagram of the injection assembly of the brain stereotactic injection apparatus of the embodiment of the present invention fitted in the fitting hole.
Fig. 4 is a schematic structural diagram of a head fixing module of a brain stereotactic injection apparatus according to an embodiment of the present invention.
Fig. 5 is a schematic structural diagram of an ear rod module of a brain stereotactic injection apparatus according to an embodiment of the present invention.
Fig. 6 is a schematic structural diagram of an injection assembly of a brain stereotactic injection apparatus of an embodiment of the present invention.
Fig. 7 is a schematic structural diagram of a leveling assembly of a brain stereotactic injection apparatus of an embodiment of the present invention.
FIG. 8 is a schematic view of the skull of an animal to be tested according to an embodiment of the present invention.
Reference numerals:
1. a housing; 11. an accommodating chamber; 12. up and down graduations; 13. a chute;
2. a positioning assembly; 21. a first scale; 211. a first scale; 22. a second scale; 221. a second scale; 23. a slider; 201. a mating hole;
3. a fixing assembly; 31. a head fixing module; 311. a column; 312. a support member; 313. a rotating shaft; 32. an ear stem module; 321. an ear mount; 322. an adjustment member; 3221. an adjustment sleeve; 3222. an adjusting block; 3223. adjusting the stud; 323. a locking member;
4. an injection assembly; 41. an injection syringe; 42. a connecting rod; 421. a pointer; 422. connecting holes; 43. a locking member;
5. a leveling assembly; 51. leveling the main body; 511. a leveling rod; 512. a leveling block; 5121. leveling the groove; 52. a level gauge; 53. a forking member; 531. a fitting portion; 532. a crotch part;
100. and (5) animals to be tested.
Detailed Description
In order to make the technical problems solved, the technical solutions adopted and the technical effects achieved by the present invention clearer, the technical solutions of the present invention are further described below by way of specific embodiments with reference to the accompanying drawings.
In the description of the present invention, it is to be understood that the terms "central," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," "circumferential," and the like are used in the orientations and positional relationships indicated in the drawings for convenience in describing the invention and to simplify the description, and are not intended to indicate or imply that the referenced device or element must have a particular orientation, be constructed and operated in a particular orientation, and are not to be considered limiting of the invention.
Furthermore, features defined as "first" and "second" may explicitly or implicitly include one or more of the features for distinguishing between descriptive features, non-sequential, non-trivial and non-trivial. In the description of the present invention, "a plurality" means two or more unless otherwise specified.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
The specific structure of the brain stereotactic injection apparatus according to the embodiment of the present invention will be described with reference to fig. 1 to 8.
As shown in fig. 1-3, the stereotactic brain injection apparatus of this embodiment includes a housing 1, a positioning component 2, a fixing component 3, an injection component 4 and a leveling component 5, the housing 1 defines a receiving cavity 11, the receiving cavity 11 is used for receiving an animal 100 to be tested, the housing 1 is provided with an upper scale 12 and a lower scale 12 extending in an up-down direction, the positioning component 2 is slidably disposed on the housing 1, the positioning component 2 is provided with a first scale 211 and a second scale 221, an extending direction of the first scale 211 is perpendicular to an extending direction of the second scale 221, the positioning component 2 is provided with a fitting hole 201, the fixing component 3 is disposed in the receiving cavity 11, the fixing component 3 is used for fixing the animal 100 to be tested in the receiving cavity 11, the injection component 4 can pass through the fitting hole 201 and extend into the receiving cavity 11, the injection component 4 is provided with a pointer 421 pointing to the upper scale 12 and the lower scale 12, the injection component 4 is used for, the leveling component 5 can pass through the matching hole 201 and extend into the accommodating cavity 11, and the leveling component 5 is used for adjusting the head of the animal 100 to be tested.
It can be understood that, in the actual use process, the animal 100 to be tested is placed in the accommodating cavity 11, the fixing component 3 is adopted to fix the animal 100 to be tested in the accommodating cavity 11, the leveling component 5 is adopted to detect the skull level degree of the animal 100 to be tested after the animal to be tested is preliminarily fixed, and the skull level of the animal 100 to be tested is enabled to be obtained by continuously adjusting the fixing component 3. Then, a marking pen penetrates through the matching hole 201, a certain biological mark point of the skull of the animal 100 to be tested is marked by the marking pen to serve as a coordinate origin, the coordinates of the first scale 211 and the second scale 221 at the moment are recorded, then the positioning assembly 2 is moved, the position of the target brain area can be found by calculating the offset distance of the matching hole 201 and the XY-axis coordinates of the coordinate origin, and the marking pen is used for making a point to be injected on the skull. Then, injection is carried out, the needle head of the injection assembly 4 is inserted into a point to be injected in the injection process, and the pointer 421 pointing to the upper and lower scales 12 is arranged on the injection assembly 4, so that the extending of the needle head can be judged according to the position of the pointer 421. Therefore, the brain stereotactic injection device of the embodiment ensures that the skull of the animal 100 to be tested can be ensured to be horizontal in the injection process and ensures that the injection can be stably and accurately performed through the interaction of the positioning component 2, the leveling component 5 and the fixing component 3. Meanwhile, the pointer 421 pointing to the upper and lower scales 12 is arranged on the injection assembly 4, so that the injection depth of the injection assembly 4 can be accurately controlled, and the injection precision can be further ensured. In addition, the brain stereotactic injection device of this embodiment's structure is very simple, and it is very convenient to operate, has promoted experimental efficiency, has reduced the experiment cost.
The brain stereotactic injection device of this embodiment, owing to be equipped with fixed subassembly 3 and leveling subassembly 5 of fixing and adjusting the animal 100 gesture of waiting to experimental, and have the locating component 2 that is used for locating injection subassembly 4 and fix a position the upper and lower scale 12 and the pointer 421 of injection depth, simplified the operation process of stereotactic injection, realized standardized, the stereotactic injection operation of accuracy, saved experimental space, promoted experimental efficiency. In addition, the whole brain stereotactic injection device has very simple structure and small volume, and better solves the problems of high instrument cost, complex operation and the like in the existing stereotactic injection experiment.
In some embodiments, as shown in fig. 1, the positioning assembly 2 includes a first scale 21, a second scale 22 and a sliding block 23, a first scale 211 is provided on the first scale 21, the second scale 22 is connected to the first scale 21, and a second scale 221 is provided on the second scale 22, the matching hole 201 is provided at the connecting end of the first scale 21 and the second scale 22, the number of the sliding blocks 23 is two, the two sliding blocks 23 are respectively sleeved on the first scale 21 and the second scale 22, and the housing 1 is provided with a sliding groove 13 matched with the sliding blocks 23. It can be understood that, in practical use, the two sliding blocks 23 are located at different positions of the housing 1 by the sliding blocks 23, and the positions of the fitting holes 201 in the two directions of the X axis and the Y axis in the spatial plane can be adjusted by sliding the first scale 21 and the second scale 22, and the positions of the fitting holes 201 in the two directions of the X axis and the Y axis in the spatial plane can be adjusted by adjusting the positions of the fitting holes 201 in the fitting holes 201, i.e. adjusting the positions of the fitting holes 5 and the injection assembly 4. Therefore, the injection assembly 4 and the leveling assembly 5 can be conveniently adjusted according to the body type of the animal 100 to be tested and the injection requirement of the test, so that the injection test is conveniently carried out, and the test efficiency is improved.
In addition, first scale 21 and second scale 22 adopt the structure of slider 23 cooperation spout 13 to realize with the relative slip of casing 1, have guaranteed the steady motion of first scale 21 and second scale 22 on the one hand, have avoided first scale 21 and second scale 22 to take place to rock betterly, and on the other hand has restricted the slip direction of first scale 21 and second scale 22, has guaranteed the accuracy when adopting first scale 211 and second scale 221 location injection subassembly 4 and leveling subassembly 5. It should be noted that, of course, in some embodiments, the housing 1 may be provided with a protrusion, and the slider 23 may be provided with a groove for matching with the protrusion.
In some embodiments, as shown in fig. 1 and 4, the fixing assembly 3 includes a head fixing module 31, the head fixing module 31 includes a column 311, a support 312 and a rotating shaft 313, the column 311 is connected to the bottom wall of the housing 1, the support 312 is used for supporting the lower jaw of the animal 100 to be tested, one end of the rotating shaft 313 is connected to the support 312, and the other end is connected to the column 311 through a screw thread. It can be understood that, in the actual use process, the rotating support 312 can adjust the head position of the animal 100 to be tested, so that the skull leveling of the animal 100 to be tested can be conveniently realized by matching with the leveling component 5. One end of the rotating shaft 313 is connected with the upright column 311 through a thread, so that the supporting piece 312 cannot rotate after rotating in place, and the skull of the animal 100 to be tested is shaken. It should be added that, in the present embodiment, the shape of the supporting member 312 can be selected from any shape capable of supporting the animal 100 to be tested, such as U-shape, L-shape, etc., according to actual needs. Here, the specific shape of the support 312 is not limited.
In some embodiments, as shown in fig. 1-3, the fixing assembly 3 further includes two ear rod modules 32, two ear rod modules 32 are respectively disposed on two opposite sidewalls of the housing 1, and the two ear rod modules 32 are configured to stop against a lower edge bone socket outside the ear canal of the animal 100 to be tested. It can be understood that, the two ear rod modules 32 cooperate with the head fixing module 31 to realize the triangular positioning, and the animal 100 to be tested can be fixed in the accommodating cavity 11 relatively fixedly, so that the injection experiment is ensured to be accurately carried out.
In some embodiments, as shown in fig. 5, each ear rod module 32 includes an ear fixing member 321, an adjusting part 322 and a locking member 323, the ear fixing member 321 is disposed on the sidewall of the housing 1, the ear fixing member 321 is provided with an ear fixing scale, the adjusting part 322 is matched with the ear fixing member 321 to adjust the height position of the ear fixing member 321, the locking member 323 is matched with the adjusting part 322, and the locking member 323 can abut against the adjusting part 322 to lock the ear fixing member 321. It can be understood that, because the adjusting component 322 can adjust the height position of the ear fixing component 321, the brain stereotactic injection apparatus of the present embodiment can adapt to animals 100 to be tested of various body types, and the application range of the brain stereotactic injection apparatus is improved. Meanwhile, the additionally arranged locking piece 323 can ensure the stability of the ear fixing piece 321 after abutting against the lower edge bone socket outside the ear canal of the animal 100 to be tested, and the phenomenon that the positioning of the animal 100 to be tested is inaccurate due to the fact that the locking ear fixing piece 321 shakes is avoided. In addition, the ear fixing scale can ensure that the animal 100 to be tested is fixed at the right center of the accommodating cavity 11, thereby facilitating subsequent operation.
In some more specific embodiments, as shown in fig. 5, the adjusting component 322 includes an adjusting sleeve 3221, an adjusting block 3222, and an adjusting stud 3223, the adjusting sleeve 3221 is fitted on the housing 1, an adjusting groove is disposed at an upper end of the adjusting sleeve 3221, an adjusting threaded hole is disposed at a lower end of the adjusting sleeve 3221, the adjusting groove is communicated with the adjusting threaded hole, the adjusting block 3222 is slidably disposed in the adjusting groove along an up-down direction, the adjusting block 3222 is sleeved on the ear fixing member 321, the adjusting stud 3223 is fitted in the adjusting threaded hole, and an upper end of the adjusting stud 3223 abuts against the adjusting block 3222. It can be understood that, in the actual use process, the height of the adjusting block 3222 can be adjusted only by rotating the adjusting stud 3223, so as to adjust the height of the ear fixing member 321. The adjusting mode is very simple, the adjustment by a user is convenient, and the experiment operation is simplified. After adjustment is finished, the locking of the ear fixing rod can be realized only by screwing the locking member 323 into the adjusting block 3222 and stopping against the ear fixing rod, so that the locking structure can ensure the stability of the ear fixing rod, and the adjustment and fixing operations of the ear fixing rod are simplified.
Of course, in other embodiments of the present invention, the ear rod module 32 may be formed as a telescopic rod installed on the housing 1, or a detachable fixed rod with different lengths, and the like, and may also perform the function of fixing the animal 100 to be tested.
In some embodiments, as shown in fig. 6, the injection assembly 4 comprises an injection syringe 41, a connecting rod 42 and a locking element 43, one end of the connecting rod 42 is provided with a connecting hole 422, the injection syringe 41 is fitted in the connecting hole 422, the other end of the connecting rod 42 is provided with a pointer 421, the connecting rod 42 is a telescopic rod, the locking element 43 is fitted on the connecting rod 42, and the locking element 43 is used for locking the injection syringe 41 on the connecting rod 42. It will be appreciated that the locking element 43 ensures the stability of the syringe 41 and avoids injection errors caused by tilting of the syringe 41 during injection. And connecting rod 42 can ensure for the telescopic link that pointer 421 stops to on upper and lower scale 12 in the injection process, has made things convenient for the user to the observation of injection depth to guarantee the injection precision.
In some specific embodiments, as shown in fig. 7, the leveling assembly 5 includes a leveling main body 51, a level 52 and two bifurcations 53, the leveling main body 51 is provided with a leveling groove 5121, the level 52 is fitted in the leveling groove 5121, the bifurcations 53 are two, both the bifurcations 53 are rotatably arranged below the leveling main body 51, and the two bifurcations 53 can be stopped on the skull of the animal 100 to be tested. It will be understood that, in actual use, the leveling body 51 is inserted through the matching hole 201 of the positioning member 2, and the positioning member 2 is adjusted in position so that the fork 53 directly acts on the skull of the animal 100 to be tested, as shown in the schematic skull diagram 8 of the animal 100 to be tested, the point a is Bregma point (Bregma point), the point B is Lambda point (Bregma point), and a seam is formed between the point a and the point B. First, the opening and closing angles of the two forks 53 are adjusted by using forceps, so that the needlepoints of the two forks 53 are aligned A, B at two corresponding positions. According to the display condition of the level 52, the supporting part 312 of the head fixing module 31 is adjusted, when the two bifurcations 532 are both contacted with the skull and the level 52 displays the level, the skull reaches the longitudinal horizontal state. Then, the opening and closing angle of the bifurcation 532 is kept unchanged, the bifurcation is twisted by 90 degrees, and the connecting line of the bifurcation 532 and the skull bottom is perpendicular to the line segment AB. When the two bifurcations 532 are again in contact with the skull bone (the contact points are two points CD in fig. 8, AB ═ CD), the height of the ear fixing member 321 is adjusted until the level 52 shows level, and the mouse head can be considered to be in the horizontal zero plane. Therefore, the leveling component 5 with the two forks 53 can conveniently realize skull leveling of the animal 100 to be tested, and the leveling precision can be improved by adopting a four-point two-line leveling mode in the leveling process, so that the injection precision is favorably improved.
It should be additionally noted that in embodiments of the present invention, the level 52 is commercially available, and the specific model parameters of the level 52 are not limited herein.
In some more specific embodiments, as shown in fig. 7, each of the bifurcations 53 includes a fitting portion 531 and a bifurcating portion 532, the fitting portion 531 is connected to the leveling member 5 by a pin, the bifurcating portion 532 is connected to the fitting portion 531, and an end of the bifurcating portion 532 remote from the fitting portion 531 is formed in a tapered shape. It is understood that the smaller the contact area of the bifurcated portion 532 with the skull of the animal 100 to be tested is, the higher the leveling accuracy is, and the formation of the tapered shape at the end of the bifurcated portion 532 remote from the fitting portion 531 enables the bifurcated member 53 to be point-contacted with the skull of the animal 100 to be tested, thereby ensuring the leveling accuracy. In the present embodiment, the specific shape of the bifurcation 532 can be any one or more combination of a cone, a pyramid or an irregular cone according to actual needs.
In some more specific embodiments, as shown in fig. 7, the leveling body 51 includes a leveling bar 511 and a leveling block 512, the leveling bar 511 can be fitted in the fitting hole 201, and the bifurcating piece 53 is fitted at the lower end of the leveling bar 511, the leveling block 512 is fitted at the upper end of the leveling bar 511, and a leveling groove 5121 is provided on the leveling block 512. It is understood that the diameter of the leveling rod 511 is limited by the fitting hole 201 in order to pass through the fitting hole 201, and the level gauge 52 has a relatively large size, and if the level gauge 52 is directly mounted on the leveling rod 511, a phenomenon occurs in which the level gauge 52 is unstable, and in this embodiment, the leveling block 512 having a relatively large volume at the upper end of the leveling rod 511 can ensure the stability of the level gauge 52, thereby ensuring the leveling accuracy.
Example (b):
a brain stereotactic injection device according to one embodiment of the present invention is described below with reference to fig. 1-7.
As shown in fig. 1, the brain stereotactic injection apparatus of the present embodiment includes a housing 1, a positioning component 2, a fixing component 3, an injection component 4 and a leveling component 5, the housing 1 defines a containing cavity 11, the containing cavity 11 is used for placing an animal 100 to be tested, the housing 1 is provided with an upper scale 12 and a lower scale 12 extending along an up-down direction, and the upper scale 12 and the lower scale are accurate to millimeters.
The shell is a cuboid box with the length of 6cm, the width of 3cm and the height of 4cm, and polypropylene with higher transparency and higher acid and alkali resistance and organic reagent degree is used as a main material for manufacturing the shell.
As shown in fig. 1, the positioning assembly 2 includes a first scale 21, a second scale 22, and a slider 23. The first scale 21 is provided with a first scale 211, and the first scale 211 is accurate to millimeters. The second scale 22 is connected to the first scale 21, and the second scale 22 is provided with a second scale 221, and the second scale 221 is accurate to millimeters. The connecting end of first scale 21 and second scale 22 is equipped with mating holes 201, and slider 23 is two, and two sliders 23 overlap respectively and establish on first scale 21 and second scale 22, be equipped with on casing 1 with slider 23 complex spout 13.
As shown in fig. 1, 4 and 5, the fixing assembly 3 is used for fixing the animal 100 to be tested in the accommodating cavity 11, and the fixing assembly 3 includes a head fixing module 31 and an ear rod module 32. The head fixing module 31 comprises a column 311, a support 312 and a rotating shaft 313, the column 311 is connected to the bottom wall of the housing 1, the support 312 is used for supporting the mandible of the animal 100 to be tested, one end of the rotating shaft 313 is connected to the support 312, and the other end is connected to the column 311 through a screw thread. The number of the ear rod modules 32 is two, the two ear rod modules 32 are respectively arranged on the two opposite side walls of the housing 1 in a penetrating manner, and the two ear rod modules 32 are used for stopping the lower edge bone socket outside the ear canal of the animal 100 to be tested. Each ear rod module 32 comprises an ear fixing member 321, an adjusting member 322 and a locking member 323, the ear fixing member 321 is arranged on the side wall of the housing 1 in a penetrating manner, and ear fixing scales are arranged on the ear fixing member 321 and are accurate to millimeters. Adjustment member 322 cooperates with ear mount 321 to adjust the height position of ear mount 321, and retaining member 323 cooperates with adjustment member 322, with retaining member 323 being capable of abutting against adjustment member 322 to retain ear mount 321. The adjusting part 322 comprises an adjusting sleeve 3221, an adjusting block 3222 and an adjusting stud 3223, the adjusting sleeve 3221 is fitted on the housing 1, an adjusting groove is formed at the upper end of the adjusting sleeve 3221, an adjusting threaded hole is formed at the lower end of the adjusting sleeve 3221, the adjusting groove is communicated with the adjusting threaded hole, the adjusting block 3222 is slidably arranged in the adjusting groove along the up-down direction, the adjusting block 3222 is sleeved on the ear fixing member 321, the adjusting stud 3223 is fitted in the adjusting threaded hole, and the upper end of the adjusting stud 3223 is stopped against the adjusting block 3222.
As shown in fig. 6, the injection assembly 4 includes an injection syringe 41, a connecting rod 42 and a locking element 43, one end of the connecting rod 42 is provided with a connecting hole 422, the injection syringe 41 is fitted in the connecting hole 422, the other end of the connecting rod 42 is provided with a pointer 421, the connecting rod 42 is a telescopic rod, the locking element 43 is fitted on the connecting rod 42, and the locking element 43 is used for locking the injection syringe 41 on the connecting rod 42.
As shown in fig. 7, the leveling assembly 5 includes a leveling main body 51, a level 52 and two bifurcations 53, the leveling main body 51 is provided with a leveling groove 5121, the level 52 is fitted in the leveling groove 5121, the number of the bifurcations 53 is two, both the bifurcations 53 are rotatably arranged below the leveling main body 51, and the two bifurcations 53 can be stopped on the skull of the animal 100 to be tested. Each branching member 53 includes a fitting portion 531 and a branching portion 532, the fitting portion 531 is connected to the leveling member 5 by a pin, the branching portion 532 is connected to the fitting portion 531, and one end of the branching portion 532, which is far from the fitting portion 531, is formed in a tapered shape. The leveling body 51 includes a leveling rod 511 and a leveling block 512, the leveling rod 511 can be fitted in the fitting hole 201, and the bifurcating member 53 is fitted at the lower end of the leveling rod 511, the leveling block 512 is fitted at the upper end of the leveling rod 511, and a leveling groove 5121 is provided on the leveling block 512.
The application method of the brain stereotactic injection device of the embodiment is as follows:
the first step is as follows: during the experiment, firstly, the mouse is weighed and anesthetized, the weight of the mouse is weighed by using an electronic balance, and 80mg/kg and 0.5-1% of pentobarbital sodium solution are injected into the abdominal cavity;
the second step is that: placing the anesthetized mouse on the bottom wall of the shell 1, using the ear fixing pieces 321 to prop against the lower marginal fossa outside the auditory canal of the mouse, and adjusting the length of the ear fixing pieces 321 at two sides (judging through ear fixing scales) to enable the head of the mouse to be positioned at the central position of the bottom wall of the shell 1;
the third step: the head fixing module 31 is adjusted to fix the head of the mouse, and the heights of the ear fixing pieces 321 and the supporting piece 312 on the two sides are adjusted to enable the head of the mouse to be in a horizontal zero plane position;
the fourth step: moistening eyeball of mouse with erythromycin eye ointment, preventing cornea from drying, cutting hair off top of head of mouse with curved scissors, sterilizing scalp with iodophor and 75% alcohol cotton ball, cutting scalp with scalpel or ophthalmology scissors along median line, exposing sagittal suture and herringbone suture, wiping with sterile absorbent cotton ball to remove fascia cranial tissue and blood, and making Bregma point (anterior fontanel point) and Lambda point (posterior fontanel point) of skull of mouse clearly visible;
the fifth step: the leveling main body 51 is passed through the matching hole 201 on the positioning component 2, and the position of the positioning component 2 is adjusted, so that the bifurcation 53 directly acts on the skull of the mouse, as shown in the schematic diagram 8 of the skull of the mouse, the point A is the Bregma point, the point B is the Lambda point, and a seam is arranged between the point A and the point B. Firstly, the opening and closing angles of the two bifurcating parts 532 are adjusted by using forceps, so that the needlepoints of the two bifurcating parts 532 are aligned to the corresponding positions of A, B two points, the supporting part 312 of the head fixing module 31 is adjusted according to the display condition of the level gauge 52, and when the two bifurcating parts 532 are both contacted with the skull and the level gauge 52 displays the level, the skull reaches the longitudinal horizontal state. Then, the opening and closing angle of the bifurcation 532 is kept unchanged, the bifurcation 532 is twisted by 90 degrees, the connecting line of the bifurcation 532 and the bottom of the skull is perpendicular to the line segment AB, when the two bifurcations 532 are contacted with the skull again (the contact points are two points of CD in fig. 8, AB is CD), the height of the ear fixing piece 321 is adjusted until the level meter 52 shows the level, and the head of the mouse can be considered to be in the horizontal zero plane;
and a sixth step: taking out the leveling component 5, replacing the leveling component 5 with a marker pen, sliding the positioning component 2, aligning a pen point with a point A, recording real-time coordinates on the first scale 211 and the second scale 221 at the moment, selecting the point as an origin of coordinates, referring to brain map coordinates, moving the positioning component 2, finding the position of a target brain area by calculating the offset distance of the matching hole 201 and the XY-axis coordinates of the point A, and marking the position on the skull by using the marker pen;
the seventh step: a mini skull drill is used for vertically drilling holes at the mark points, and the holes are hollow when drilled through (whether the skull drill is used or not can be selected according to the experimental condition, for example, the skull can be directly punctured by using a needle when the medicine is administered in a ventricle so as to achieve the experimental purpose);
eighth step: the sample was aspirated using syringe 41 and the needle was sterilized with 75% alcohol. The injection needle cylinder 41 penetrates through the matching hole 201, then the needle head is aligned to a skull window, the needle point descends to be tangent to the surface of the skull, the connecting rod 42 is clamped on the injection needle cylinder 41, the length of the connecting rod 42 is adjusted, the pointer 421 is enabled to be tightly attached to the upper scale 12 and the lower scale 12 on the wall, and the corresponding Z-axis coordinate at the moment is recorded;
the ninth step: according to the coordinates provided by the brain map, slowly sinking the needle head to the target area, descending the connecting rod 42 along with the descending of the injection needle cylinder 41, and controlling the needle inserting depth through the distance of the pointer 421 moving on the upper and lower scales 12;
the tenth step: dropping physiological saline on the opening of the skull with a disposable syringe to seal, preventing the tissue from drying, slowly injecting the injection sample into the target spot, stopping the injection for 10-15 min after the injection is finished, fully diffusing the sample, and slowly lifting the injection syringe 41 after the injection is finished to prevent the sample liquid from oozing due to overhigh speed;
the eleventh step: the scalp was sutured with angle needles and sterile sutures and the pain and inflammation were relieved using lidocaine lincomycin gel, and the mice were placed on a heat blanket to maintain body temperature until awakened and then returned to the home cage.
The intracerebral stereotactic injection device of the embodiment can realize standardized and accurate intracranial injection of a mouse, and compared with the intracerebral stereotactic injection operation which can be carried out only by means of a laboratory table and a brain stereotaxic apparatus in the prior art, the intracerebral stereotactic injection device of the embodiment has low requirements on experimental environment, reduces the skull leveling time and the target nucleus positioning time of the mouse, reduces the operation difficulty coefficient of the device, and improves the experimental efficiency. The experimental effect is obvious especially for intracranial injection of large nuclei such as hippocampus, cortex, ventricles and the like. The intracerebral stereotactic injection device of the embodiment is made of polypropylene, is low in production cost, easy to process and capable of being produced in batches, and can meet the experiment requirements of more experimenters.
In addition, it should be noted that, in the description of this embodiment, the stereotactic injection of a mouse is taken as an example, and the embodiment of this embodiment is specifically expressed in detail, and in the actual use process, the device is not limited to the stereotactic injection in the brain of a mouse, but the device can be used in the stereotactic injection in the brain of a relatively small animal.
In the description herein, references to the description of "some embodiments," "other embodiments," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The above description is only a preferred embodiment of the present invention, and for those skilled in the art, the present invention should not be limited by the description of the present invention, which should be interpreted as a limitation.
Claims (10)
1. A brain stereotactic injection device, comprising:
the test device comprises a shell (1), wherein the shell (1) defines an accommodating cavity (11), the accommodating cavity (11) is used for placing an animal (100) to be tested, and the shell (1) is provided with an upper scale (12) and a lower scale (12) which extend along the vertical direction;
the positioning assembly (2) is slidably arranged on the shell (1), a first scale (211) and a second scale (221) are arranged on the positioning assembly (2), the extending direction of the first scale (211) is perpendicular to the extending direction of the second scale (221), and a matching hole (201) is formed in the positioning assembly (2);
the fixing component (3), the fixing component (3) is arranged in the accommodating cavity (11), and the fixing component (3) is used for fixing the animal (100) to be tested in the accommodating cavity (11);
the injection assembly (4) can penetrate through the matching hole (201) and extend into the accommodating cavity (11), a pointer (421) pointing to the upper scale and the lower scale (12) is arranged on the injection assembly (4), and the injection assembly (4) is used for injecting an experimental reagent to the animal (100) to be tested;
leveling subassembly (5), leveling subassembly (5) can pass mating holes (201) and stretch into hold chamber (11), leveling subassembly (5) are used for the adjustment treat the head of experimental animal (100).
2. The brain stereotactic injection apparatus of claim 1, wherein said positioning assembly (2) comprises:
the first scale (21), the first scale (211) is arranged on the first scale (21);
the second scale (22), the second scale (22) is connected with the first scale (21), the second scale (22) is provided with the second scale (221), and the matching hole (201) is formed in the connecting end of the first scale (21) and the second scale (22);
slider (23), slider (23) are two, two slider (23) overlap respectively and establish first scale (21) with on second scale (22), be equipped with on casing (1) with slider (23) complex spout (13).
3. The brain stereotactic injection apparatus according to claim 1, wherein said fixation assembly (3) comprises a head fixation module (31), said head fixation module (31) comprising:
the upright post (311) is connected to the bottom wall of the shell (1);
a support (312), the support (312) being for supporting the mandible of the animal (100) to be tested;
and one end of the rotating shaft (313) is connected with the supporting piece (312), and the other end of the rotating shaft (313) is connected with the upright column (311) through threads.
4. The brain stereotactic injection apparatus of claim 1, wherein said mounting assembly (3) further comprises two ear bar modules (32), said two ear bar modules (32) are respectively disposed through two opposite sidewalls of said housing (1), said two ear bar modules (32) are configured to stop against the lower edge bone socket outside the ear canal of said animal (100) to be tested.
5. The brain stereotactic injection apparatus of claim 4, wherein each of said ear stem modules (32) comprises:
the ear fixing piece (321) is arranged on the side wall of the shell (1) in a penetrating mode, and ear fixing scales are arranged on the ear fixing piece (321);
an adjustment component (322), the adjustment component (322) cooperating with the ear mount (321) to adjust a height position of the ear mount (321);
a locking member (323), the locking member (323) fitting over the adjustment member (322), the locking member (323) being capable of resting against the adjustment member (322) to lock the ear mount (321).
6. The brain stereotactic injection apparatus of claim 5, wherein said adjustment member (322) comprises:
the adjusting sleeve (322) is matched on the shell (1), an adjusting groove is formed in the upper end of the adjusting sleeve (322), an adjusting threaded hole is formed in the lower end of the adjusting sleeve, and the adjusting groove is communicated with the adjusting threaded hole;
the adjusting block (3222) is slidably arranged in the adjusting groove along the up-down direction, and the adjusting block (3222) is sleeved on the ear fixing part (321);
the adjusting stud (3223) is matched in the adjusting threaded hole, and the upper end of the adjusting stud (3223) is stopped against the adjusting block (3222).
7. The brain stereotactic injection apparatus of claim 1, wherein said injection assembly (4) comprises:
an injection cylinder (41);
the needle injection device comprises a connecting rod (42), wherein one end of the connecting rod (42) is provided with a connecting hole (422), the injection needle cylinder (41) is matched in the connecting hole (422), the other end of the connecting rod (42) is provided with a pointer (421), and the connecting rod (42) is a telescopic rod;
a locking piece (43), the locking piece (43) is matched on the connecting rod (42), and the locking piece (43) is used for locking the injection syringe (41) on the connecting rod (42).
8. The brain stereotactic injection apparatus of claim 1, wherein said leveling assembly (5) comprises:
the leveling device comprises a leveling main body (51), wherein a leveling groove (5121) is arranged on the leveling main body (51);
a level (52), the level (52) fitting within the leveling slot (5121);
the leveling device comprises two forking pieces (53), the two forking pieces (53) are both rotatably arranged below the leveling main body (51), and the two forking pieces (53) can be stopped on the skull of the animal (100) to be tested.
9. The brain stereotactic injection apparatus of claim 8, wherein each of said bifurcations (53) comprises:
the matching part (531), the matching part (531) is connected with the leveling component (5) through a pin shaft;
a bifurcated part (532), the bifurcated part (532) is connected with the matching part (531), and one end of the bifurcated part (532) far away from the matching part (531) is formed into a conical tip shape.
10. The brain stereotactic injection apparatus of claim 8, wherein said leveling body (51) comprises:
a leveling lever (511), the leveling lever (511) being capable of fitting within the fitting hole (201), and the bifurcating member (53) fitting at a lower end of the leveling lever (511);
the leveling rod (511) comprises a leveling block (512), the leveling block (512) is matched with the upper end of the leveling rod (511), and the leveling groove (5121) is formed in the leveling block (512).
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| CN202010187121.7A CN111227988A (en) | 2020-03-17 | 2020-03-17 | Brain stereotactic injection device |
| PCT/CN2020/084440 WO2021184462A1 (en) | 2020-03-17 | 2020-04-13 | Brain stereotactic injection device |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010187121.7A CN111227988A (en) | 2020-03-17 | 2020-03-17 | Brain stereotactic injection device |
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| CN111227988A true CN111227988A (en) | 2020-06-05 |
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Family Cites Families (9)
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| CN108294842B (en) * | 2018-01-08 | 2020-03-24 | 合肥龙图腾信息技术有限公司 | Swiftly give dog appurtenance that injects |
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