CN111226941B - Botanical insecticide for preventing and controlling crop pests - Google Patents
Botanical insecticide for preventing and controlling crop pests Download PDFInfo
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- CN111226941B CN111226941B CN202010094268.1A CN202010094268A CN111226941B CN 111226941 B CN111226941 B CN 111226941B CN 202010094268 A CN202010094268 A CN 202010094268A CN 111226941 B CN111226941 B CN 111226941B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
Abstract
The invention discloses a botanical pesticide for preventing and controlling crop pests, which comprises nicotine citrate as an active ingredient. The insecticidal composition shows higher biological activity to most of piercing-sucking mouthpart pests, and can be used for development and application of biological insecticides for effectively preventing and controlling crop pests.
Description
Technical Field
The invention belongs to the technical field of biological pesticides, and particularly relates to a botanical pesticide for preventing and controlling crop pests.
Background
The plant-derived pesticide refers to a pesticide whose active ingredient is derived from a plant. They are generally classified as biopesticides or biogenic pesticides along with microbial or biochemical pesticides. Along with the increasing health consciousness and environmental consciousness of the public, chemical pesticides bring a plurality of negative effects on agricultural production due to the problems of environmental pollution, high residue and the like. The biological pesticide has the advantages of easy degradation in environment, safety to human and livestock, strong selectivity and the like, and meets the requirement of sustainable development of green agriculture.
Nicotine (Nicotine) is derived from plants of the genus Nicotiana (Nicotiana) of the family Solanaceae (Solanaceae), and is the major alkaloid of tobacco. The average nicotine content in tobacco is 1-4%, and accounts for about 95% of total alkaloid content in tobacco. The use of nicotine as a botanical pesticide, having fumigant, contact and stomach poisoning effects on crop pests, is registered as a biopesticide. The nicotine is easy to volatilize and oxidize, so that the nicotine has low persistence and low stability in the field use process, and a nicotine preparation cannot be effectively developed and applied. The nicotine is mainly used for electronic cigarette, biological medicine research and the like at present, and our experimental research finds that the citric acid nicotine has stronger and faster insecticidal effect than nicotine and good preparation stability, so that the development of the citric acid nicotine as a biological insecticide has important application prospect and practical value.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention provides the botanical pesticide for preventing and controlling the crop pests, which is environment-friendly, high in safety, high in pesticide effect showing speed and good in pesticide effect.
The technical scheme adopted by the invention for solving the technical problems is as follows: a botanical pesticide for controlling crop pests, which comprises the following active ingredients of nicotine citrate with the structural formula I:
Preferably, n in the structural formula I is 1, 1.5, 2 or 3.
Preferably, the weight of the nicotine citrate is 0.1-99.9% of the total weight of the pesticide.
Preferably, the weight of the nicotine citrate accounts for 1-90% of the total weight of the pesticide.
Preferably, the crop pests are sucking mouth parts pests.
Further, the crop pests are brown planthopper, white-backed planthopper, laodelphax striatellus, bemisia tabaci, aphid, thrips, leaf miner, phyllotreta striolata, Chinese chive maggot and root-knot nematode.
Preferably, the dosage form is conventional preparation, including soluble solution, missible oil, microemulsion, oil suspending agent, dispersible granule, microcapsule suspending agent, seed dressing agent, seed coating agent and granule.
Preferably, the preparation form is a soluble solution, and comprises 1-20 parts of nicotine citrate, 2-5 parts of solvent, 1-5 parts of emulsifier, 1-5 parts of cosolvent and 65-95 parts of water in parts by mass; the solvent is one or more of ethanol, glycol, acetone, n-propanol, isopropanol, ethylene glycol methyl ether, ethylene glycol ethyl ether, ethylene glycol dimethyl ether and diethylene glycol; the emulsifier is one or more of fatty alcohol polyoxyethylene ether, alkylphenol polyoxyethylene polyoxypropylene ether, fatty acid polyoxyethylene ester, castor oil polyoxyethylene ether, fatty amine polyoxyethylene ether, propylene glycol block polyether, random block polyether, sorbitan fatty acid ester polyoxyethylene ether, polyoxyethylene sorbitan fatty acid ester, tristyrylphenol polyoxypropylene polyoxyethylene block polymer, fatty alcohol EO/PO block copolymer, alkyl glycoside, castor oil phosphate, fatty alcohol polyoxyethylene ether organic phosphate, polyoxyethylene polyoxypropylene block copolymer, calcium dodecyl benzene sulfonate, sodium dodecyl sulfate, phenethyl phenol polyoxyethylene ether, phenethyl phenol polyoxyethylene polyoxypropylene ether and alkylphenol formaldehyde resin polyoxyethylene ether; the cosolvent is one or more of ethanol, ethylene glycol, glycerol, isopropanol, n-butanol, isobutanol and polyethylene glycol.
Preferably, the preparation form is a microemulsion and comprises 1-20 parts of nicotine citrate, 1-10 parts of solvent, 2-10 parts of emulsifier, 1-5 parts of cosolvent and 55-95 parts of water in parts by mass; the solvent is one or more of limonene, cyclohexanone, propylene glycol, dipropylene glycol, ethylene glycol methyl ether and ethylene glycol ethyl ether; the emulsifier is one or more of fatty alcohol polyoxyethylene ether, alkylphenol polyoxyethylene polyoxypropylene ether, fatty acid polyoxyethylene ester, castor oil polyoxyethylene ether, fatty amine polyoxyethylene ether, propylene glycol block polyether, random block polyether, sorbitan fatty acid ester polyoxyethylene ether, polyoxyethylene sorbitan fatty acid ester, tristyrylphenol polyoxypropylene polyoxyethylene block polymer, fatty alcohol EO/PO block copolymer, alkyl glycoside, castor oil phosphate, fatty alcohol polyoxyethylene ether organic phosphate, polyoxyethylene polyoxypropylene block copolymer, calcium dodecyl benzene sulfonate, sodium dodecyl sulfate, phenethyl phenol polyoxyethylene ether, phenethyl phenol polyoxyethylene polyoxypropylene ether and alkylphenol formaldehyde resin polyoxyethylene ether; the cosolvent is one or more of ethanol, ethylene glycol, glycerol, isopropanol and polyethylene glycol.
Preferably, the formulation is missible oil, and comprises 1-20 parts of nicotine citrate, 2-10 parts of emulsifier and 70-97 parts of solvent in parts by mass; the emulsifier is one or more of phenylethylphenol formaldehyde resin polyoxyethylene ether, phenylethylphenol polyoxyethylene polyoxypropylene ether, fatty alcohol polyoxyethylene ether, alkylphenol polyoxyethylene polyoxypropylene ether, fatty acid polyoxyethylene ester, castor oil polyoxyethylene ether, fatty amine polyoxyethylene ether, propylene glycol block polyether, random block polyether, sorbitan fatty acid ester, polyoxyethylene sorbitan fatty acid ester, fatty alcohol EO/PO block copolymer, alkyl glycoside, castor oil phosphate ester, fatty alcohol polyoxyethylene ether organic phosphate ester, polyoxyethylene polyoxypropylene block copolymer, calcium dodecylbenzene sulfonate, sodium dodecyl sulfate and alkylphenol formaldehyde resin polyoxyethylene ether; the solvent is one or more of turpentine-based vegetable oil, fatty acid methyl ester, methyl oleate, ethyl oleate, lauryl oleate, rosin methyl ester, hydrogenated rosin methyl ester, rosin polyoxyethylene ester, sec-butyl acetate, gamma-butyrolactone, N-alkyl-pyrrolidone, acetophenone, dimethyl carbonate, dipropyl carbonate, imidazolone solvents, dimethyl octanoamide, dimethyl decanoamide, C8-C12 acid dimethylamide, C12 acid dimethylamide, C18 acid dimethylamide, lactic acid dimethylamide, mixed fatty chain (C6-C12) morpholinamide, isohexyl bisdimethylamide and solvent oil.
Preferably, the formulation is a seed dressing agent and comprises 1-15 parts of nicotine citrate, 1-5 parts of a dispersing agent, 2-8 parts of a wetting agent, 0.5-4 parts of a film forming agent, 0.1-0.5 part of warning color, 0-5 parts of an antifreezing agent and 62.5-95.4 parts of deionized water in parts by mass; the dispersing agent is one or more of polyoxyethylene ether, triethylhexyl phosphoric acid, sodium dodecyl sulfate, alkyl naphthalene naphthenate formaldehyde condensate, cresol sulfonic acid, sodium naphthalene sulfonate formaldehyde condensate, lignosulfonate, alkylphenol polyoxyethylene ether formaldehyde condensate sulfate, fatty alcohol polyoxyethylene ether sulfate, alkyl phosphate, fatty alcohol polyoxyethylene ether organic phosphate and polycarboxylate; the wetting agent is one or more of polyacrylate, nekal, polyoxyethylene alkylphenol ether, polyoxyethylene fatty alcohol ether, polyoxyethylene polyoxypropylene block copolymer, alkyl sulfate, alkyl naphthalene naphthenate, sulfonate, fatty acid ester sulfate and fatty alcohol polyoxyethylene ether organic phosphate; the film forming agent is one or more of sodium alginate, sodium carboxymethyl cellulose, soluble starch, gelatin and polyvinyl alcohol; the warning color is one or more of ponceau, gamboge, alizarin red, indigo, malachite green and cochineal red; the antifreezing agent is one or more of ethanol, ethylene glycol, propylene glycol, diethylene glycol, glycerol, polyethylene glycol and urea.
Preferably, the formulation is granules and comprises, by mass, 0.5-5 parts of nicotine citrate, 1-8 parts of wetting agent, 0.1-5 parts of dispersing agent, 0.1-4 parts of binding agent and 78-98.3 parts of carrier; the wetting agent is one or more of polyacrylate, nekal, polyoxyethylene alkylphenol ether, polyoxyethylene fatty alcohol ether, polyoxyethylene polyoxypropylene block copolymer, alkyl sulfate, sulfonate, fatty acid ester sulfate and fatty alcohol polyoxyethylene ether organic phosphate; the dispersing agent is one or more of fatty alcohol-polyoxyethylene ether, triethylhexyl phosphoric acid, sodium dodecyl sulfate, alkyl naphthalene naphthenate, an alkyl naphthalene naphthenate formaldehyde condensate, cresol sulfonic acid, a naphthol sulfonic acid formaldehyde condensate, lignosulfonate, alkylphenol polyoxyethylene ether formaldehyde condensate sulfate, fatty alcohol-polyoxyethylene ether sulfate, alkyl phosphate, fatty alcohol-polyoxyethylene ether phosphate and polycarboxylate; the binder is one or more of polyvinyl alcohol, polyethylene glycol, soluble starch, beta-cyclodextrin, gum arabic, soybean protein, gelatin and polyvinyl acetate; the carrier is one or more of calcium carbonate, white carbon black, perlite, vermiculite, attapulgite, silica sand, silica, talc, pyrophyllite, pumice and artificial granular carriers.
Preferably, the formulation is an oil suspending agent and comprises 1-20 parts of nicotine citrate, 1-20 parts of emulsifier, 1-10 parts of dispersant, 0.5-2 parts of thickener, 1-10 parts of adsorbent and 38-95.5 parts of vegetable oil or mineral oil in parts by mass; the emulsifier is one or more of polyester compound, phenethyl phenol formaldehyde resin polyoxyethylene ether, phenethyl phenol polyoxyethylene polyoxypropylene ether, fatty alcohol polyoxyethylene ether, alkylphenol polyoxyethylene polyoxypropylene ether, fatty acid polyoxyethylene ester, castor oil polyoxyethylene ether, fatty amine polyoxyethylene ether, propylene glycol block polyether, random block polyether, sorbitan fatty acid ester, polyoxyethylene sorbitan fatty acid ester, fatty alcohol EO/PO block copolymer, polyethylene oxide block copolymer, alkyl glycoside, castor oil phosphate ester, fatty alcohol polyoxyethylene ether organic phosphate ester and polyoxyethylene polyoxypropylene block copolymer; the dispersant is one or more of polycarboxylate, naphthalene sulfonate, sodium lignin sulfonate and organic phosphate; the thickening agent is one or more of organic bentonite, magnesium aluminum silicate, xanthan gum and polyvinyl alcohol; the adsorbent is one or more of white carbon black, pyrophyllite, talcum powder, light calcium carbonate, kaolin and diatomite; the oil is one or more of turpentine, soybean oil, rapeseed oil, castor oil, palm oil, corn oil, epoxidized soybean oil, methyl oleate, ethyl oleate, butyl oleate, mineral oil, rosin-based vegetable oil, agricultural solvent complex ester, coconut oil methyl ester, rapeseed methyl oleate, methyl ester soybean oil and palm methyl oleate.
Reacting nicotine and citric acid according to a certain proportion to synthesize nicotine citrate, carrying out indoor bioassay tests on brown planthopper and bemisia tabaci by using compounds obtained by the reaction, and detecting the biological activities of the compounds on two pests with piercing-sucking mouthparts; and further performing field pesticide effect tests according to indoor bioassay results, verifying the effect of the pesticide composition in the field, and providing technical support for later popularization and application.
The invention can be used for preventing and controlling pests with piercing-sucking mouthparts, and the specific crop pests can be rice brown planthopper, sogatella furcifera, laodelphax striatellus, bemisia tabaci, diaphorina citri, phyllotreta striolata, flea beetle, chive maggot and other pests; the crops acted by the invention are cereal rice, corn and wheat; vegetables including cucumber, pepper, eggplant, cabbage, cauliflower, Chinese cabbage, green vegetable, and Chinese chive; cowpea, pea, broad bean, soybean, peanut, radish; fruits such as apple, pear, peach, orange, strawberry, and bayberry; or cotton, chrysanthemum, tea tree, rape, etc.
The pesticide of the present invention is used in a general conventional formulation form, and is applied by spraying, watering, broadcasting, dressing, etc. depending on the nature of the target pest, and the amount of the effective active ingredient to be applied varies depending on weather conditions, crop conditions, or application methods.
The botanical pesticide provided by the invention has higher biological activity on most piercing-sucking mouthpart pests, has obvious inhibition effect on feeding and growth and development of lepidoptera pests, and can be developed and applied to control various crop pests as a biological pesticide.
Compared with the existing nicotine and nicotine sulfate insecticides, the botanical insecticide provided by the invention has higher plant imbibition and insecticidal activity, faster insecticidal effect and good persistent effect, and is low in toxicity to human and livestock and safe to bees.
Detailed Description
In order to make the technical solutions of the present invention better understood, the technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
A botanical pesticide for preventing and treating crop pests contains the following effective components
Nicotine citrate of (2).
In the embodiment, 5% of nicotine citrate active ingredient is weighed, 3% of ethanol solvent is added, 2.5% of polyoxyethylene sorbitan fatty acid ester serving as an emulsifier, 1.5% of fatty alcohol-polyoxyethylene ether and 2% of glycol serving as a cosolvent are added, and the mixture is stirred until the emulsifier is dissolved and is uniformly mixed with other ingredients; finally adding deionized water to complement 100%, stirring for 10min-20min, and mixing uniformly to obtain 5% nicotine citrate soluble solution.
The preparation method of nicotine citrate is known in the art and is not described in detail.
Example 2
A botanical pesticide for preventing and treating crop pests contains the following effective components
In the embodiment, 5% of nicotine citrate active ingredient is weighed, 3% of fatty alcohol-polyoxyethylene ether serving as an emulsifier and 1% of polyethylene oxide block copolymer are added, 4% of cyclohexanone serving as a solvent and 2% of polyethylene glycol serving as a cosolvent are added, the mixture is stirred until the emulsifier is dissolved and is uniformly mixed with other ingredients, deionized water is added to complement 100%, and the mixture is stirred for 10-20 min and is uniformly mixed to obtain the 5% nicotine citrate microemulsion.
Example 3
A botanical pesticide for preventing and treating crop pests contains the following effective components
In the embodiment, 10% of active ingredients of the nicotine citrate are weighed, 1% of calcium dodecyl benzene sulfonate serving as an emulsifier, 3.5% of phenethyl phenol polyoxyethylene ether and 1.5% of polyethylene oxide block copolymer are added, 4% of ethyl oleate and 100% of solvent oil are added, and the mixture is stirred for 10min to 20min and uniformly mixed to obtain 10% of nicotine citrate emulsifiable solution.
Example 4
A botanical pesticide for preventing and treating crop pests contains the following effective components
In the embodiment, 8% of the active ingredients of the citric acid nicotine are weighed, 3.5% of a dispersant alkyl naphthalene naphthenate formaldehyde condensate, 1.5% of a wetting agent alkyl naphthalene naphthenate, 0.3% of a thickening agent xanthan gum, 1% of an antifreeze glycerol and deionized water are added to make up to 100%, the mixture is stirred and mixed, then the mixture is placed in a high-shear machine, the rotation speed of the shear machine is adjusted to 6000r/min, the mixture is sheared for 15min, and the 8% citric acid nicotine seed dressing agent is obtained.
Example 5
A botanical pesticide for preventing and treating crop pests contains the following effective components
Nicotine citrate of (2).
In the embodiment, 1% of nicotine citrate active ingredient is weighed, 0.5% of nekal wetting agent and 1.5% of fatty alcohol-polyoxyethylene ether serving as a dispersing agent are added and stirred for mixing, 2.5g of 10% polyvinyl alcohol aqueous solution (with the polyvinyl alcohol content being 0.25%) is added and stirred for 10min to form uniform mixed liquid, the artificial granular carrier is weighed to complement the content to 100%, the mixed liquid is slowly added to 15% -25% of the total amount of the carrier under the stirring condition and stirred for 5min to be mixed uniformly, the carrier is continuously added and stirred for 5min to be mixed uniformly, finally the rest carrier is added and stirred for 20min to be mixed uniformly, the mixed liquid is heated to 40 ℃, ventilated and stirred until the moisture is evaporated to dryness, and the 1% nicotine citrate granule is obtained.
Example 6
A botanical pesticide for preventing and treating crop pests contains the following effective components
Nicotine citrate of (2).
In the embodiment, 5% of nicotine citrate active ingredient is weighed, 5% of adsorbent white carbon black is added, and the mixture is stirred and mixed to be dry powder for later use; weighing 2% of emulsifier fatty alcohol-polyoxyethylene ether and 15% of polyester compound, stirring for 10min, uniformly mixing, adding 3% of dispersant polycarboxylate and 1% of thickener organic bentonite, stirring for 10min, uniformly mixing to prepare an emulsified dispersed phase, adding 20% of methyl ester soybean oil into the mixture of nicotine citrate and white carbon black, uniformly stirring and mixing, placing the mixture in a shearing machine for shearing at 6000 rpm for 5min, weighing 100% of methyl ester soybean oil enough-supplementing preparation, slowly adding the methyl ester soybean oil into the preparation being sheared, accelerating the rotating speed of the shearing machine to 10000 rpm, and continuously shearing for 15min to obtain the 5% of citric acid nicotine oil suspending agent.
Indoor bioassay example 1
In the experiment, nicotine with the purity of 99.5 percent, nicotine citrate synthesized in a laboratory (the molar ratio is 1:1) and trinicotine citrate (the molar ratio is 1:3) are respectively adopted; the preparation used in the invention is prepared by the laboratory; the test insect brown planthopper (Nilaparvatalgens (Stal)) was supplied from rice in China.
The test method comprises the following steps: after soil at the root of the rice seedling is washed away, overlong leaves are removed, 2-3 plants are placed in a 250mL disposable plastic cup, and the root is fixed by 1% agar.
Soaking the rice seedlings in the treatment liquid medicine with each concentration for 20 seconds, naturally drying, and pouring agar again to seal the surface of the agar contacting the liquid medicine during soaking. And (3) inoculating brown planthopper third-instar nymphs, inoculating 30 beetles to each cup, and repeating the steps for each treatment.
Then putting the mixture into an illumination incubator for culture at the temperature of 30 ℃, and illuminating: the darkness is 16h:8h, and the mortality rate of the test insects is investigated after 48h of medicament treatment.
The test results are shown in table 1:
TABLE 1 indoor bioactivity assay results (LC) of three nicotinic compounds against Nilaparvata lugens for 48h50)
The test results show that both newly synthesized nicotine citrates have insecticidal activity on the brown planthopper and are higher than the biological activity of nicotine on the brown planthopper. The insecticidal activity of the trinicotine citrate on the brown planthopper is the strongest, and compared with nicotine, the insecticidal activity is improved by 2.6 times. In this experiment, the brown planthopper began to die 5 hours after the brown planthopper was inoculated. The compound is proved to have good quick killing performance on brown planthopper.
Indoor bioassay example 2
The nicotine raw material adopted in the test is provided for production of Hubei and Noo bioengineering GmbH (the purity is 99.5%), and the nicotine citrate is synthesized by the laboratory and respectively comprises nicotine citrate (the molar ratio is 1:1) and trinicotine citrate (the molar ratio is 1: 3); the preparation used in the invention is prepared by the laboratory; the tested insect Bemisia tabaci (Bemisiatabaci (Gennadius)) was collected in the field of Deqing county, Huzhou, and cultured and propagated indoors.
The test method comprises the following steps: adopts a finger-shaped pipe medicine film method. Three medicaments are prepared into 2.5g/L concentration by ethanol to be used as mother liquor, the mother liquor is diluted into 6 liquid medicines with different concentrations according to 2 times of gradient, simultaneously ethanol blank control is set, and each treatment is set for 3 times.
And (3) sucking 500mL of liquid medicine by using a liquid transfer gun, injecting the liquid medicine into the bottom of the finger-shaped pipe, carefully inclining and rotating the finger-shaped pipe, fixing the liquid medicine on the inner wall of the finger-shaped pipe along with the volatilization of ethanol to enable the liquid medicine to uniformly form a medicine film on the pipe wall, and inoculating insects after the medicine film is completely dried.
The plant is tapped to insert the bemisia tabaci adults into the tube, and the cotton plug is pushed into the upper edge of the pesticide film.
After treatment, the finger-shaped tube is placed in an incubator at the temperature of (25 +/-1) DEG C upside down, the death rate of bemisia tabaci is checked after 3h, and the virulence regression equation and the confidence limit of the three kinds of nicotine are calculated.
The test results are shown in table 2:
TABLE 2 statistical results (LC) of the indoor biological activity of three nicotinic compounds against Bemisia tabaci50)
The test results show that the insecticidal activity of two newly synthesized nicotine citrates on the bemisia tabaci and the brown planthopper is higher than that of nicotine, wherein the insecticidal activity of the trinicotinic citrate on the brown planthopper is strongest, and the nicotine citrate is inferior.
Field test example 1
The test is carried out in 9 months in 2018 in Tongyou 9 village of Bailongqiangzhen town in Wutown of Jinhua, Zhejiang, the test variety is Yongyou No. 9, the test is totally 4 treatments, the area of a large area is 400-600 m2, the pesticide is sprayed for 1 time, the base number is investigated before the pesticide, and the control effect is investigated 3 days, 7 days and 13 days after the pesticide.
The test results are shown in table 3:
table 310% nicotine citrate (III) soluble Agent (AS) field efficacy test result for preventing and controlling rice planthopper
Note: the treatment control effect is the average value of each repetition; capital letters indicate 1% significance of difference and lowercase letters indicate 5% significance of difference
The field test shows that: from the overall prevention effect, the test medicament 10% of the nicotine citrate soluble solution (AS) has better effect when the dosage is 600g a.i./hm2, the prevention effect reaches 73.6% 3d after the medicament is applied, the prevention effect is higher than that of the test medicament 10% of the nicotine citrate soluble solution (AS) when the dosage is 300g a.i./hm2, but the two are not obviously different; the control agent, namely 21% thiamethoxam suspending agent (SC), is 31.5g of a.i./hm2, so that the control effect is poor, and is 28.4% which is obviously lower than that of the test agent and the test agent.
7d and 13d after the drug is taken, the test medicament 10% of the nicotine citrate can be treated by solution with the dosage of 300 and 600g of a.i./hm2, and still has relatively good prevention effect, which indicates that the medicament has longer lasting effect; and the control medicament thiamethoxam 31.5g a.i./hm2 has obviously reduced control effect at 7d and 13 d.
Field test example 2
The test was carried out in 2019 in 10 months in Shuqing county, Shuangxi village, Hu, Zhejiang, and the test crop cauliflower was cultivated in open field. The experiment was set up with 4 treatments, 3 replicates per treatment. Cell random block arrangement with a cell area of 22m2(4 m.times.5.5 m). Investigating the population base number of the bemisia tabaci before spraying and applying the pesticide, investigating the residual quantity of the bemisia tabaci in each cell in 3d after the pesticide is applied, and calculating the correction control effect. The test investigation adopts a five-point sampling method, 1 plant is investigated at each point in a fixed point manner, and all leaves are investigated.
The test results are shown in Table 4:
table 410% nicotine citrate (III) soluble Agent (AS) field efficacy test results for controlling bemisia tabaci
Note: the treatment control effect is the average value of each repetition; capital letters indicate 1% differential significance and lowercase letters indicate 5% differential significance.
The field test result shows that: from the overall control effect, the test medicament 10 percent nicotine citrate soluble solution (AS) dosage is 150g a.i./hm2The effect is best, the control effect reaches 74.8 percent after 3 days of the medicine, and the control effect is higher than 37.5g a.i./hm2Treatment and 70g a.i./hm2Treating with 15g of 10% imidacloprid Wettable Powder (WP) and a.i./hm of a control medicament2Compared with the control effect, the control effect is not obviously different.
The foregoing detailed description is intended to illustrate and not limit the invention, which is intended to be within the spirit and scope of the appended claims, and any changes and modifications that fall within the true spirit and scope of the invention are intended to be covered by the following claims.
Claims (9)
1. The application of a botanical pesticide for preventing and controlling agricultural pests is characterized in that the active ingredient of the botanical pesticide is nicotine citrate with the structural formula I:
structural formula I;
in the structural formula I, n is 1, 1.5, 2 or 3, when n is 1, the compound is nicotine citrate, when n is 1.5, the compound is tricine citrate, when n is 2, the compound is bicine citrate, and when n is 3, the compound is tricine citrate;
the weight of the nicotine citrate accounts for 0.1 to 99.9 percent of the total weight of the pesticide;
the agricultural pests are selected from brown planthopper, sogatella furcifera, laodelphax striatellus, bemisia tabaci, aphid and thrips.
2. Use of a botanical pesticide as claimed in claim 1 for controlling an agricultural pest, characterized in that: the weight of the nicotine citrate accounts for 1-90% of the total weight of the pesticide.
3. Use of a botanical pesticide as claimed in claim 1 for controlling an agricultural pest, characterized in that: the dosage form of the botanical insecticide is soluble solution, missible oil, microemulsion, oil suspending agent, dispersible granule, microcapsule suspending agent, seed dressing agent, seed coating agent and granule.
4. Use of a botanical pesticide as claimed in claim 1 for controlling an agricultural pest, characterized in that: the botanical pesticide is in a soluble solution form and comprises, by mass, 1-20 parts of nicotine citrate, 2-5 parts of a solvent, 1-5 parts of an emulsifier, 1-5 parts of a cosolvent and 65-95 parts of water; the solvent is one or more of ethanol, glycol, acetone, n-propanol, isopropanol, ethylene glycol methyl ether, ethylene glycol ethyl ether, ethylene glycol dimethyl ether and diethylene glycol; the emulsifier is one or more of fatty alcohol polyoxyethylene ether, alkylphenol polyoxyethylene polyoxypropylene ether, fatty acid polyoxyethylene ester, castor oil polyoxyethylene ether, fatty amine polyoxyethylene ether, propylene glycol block polyether, random block polyether, sorbitan fatty acid ester polyoxyethylene ether, polyoxyethylene sorbitan fatty acid ester, tristyrylphenol polyoxypropylene polyoxyethylene block polymer, fatty alcohol EO/PO block copolymer, alkyl glycoside, castor oil phosphate, fatty alcohol polyoxyethylene ether organic phosphate, polyoxyethylene polyoxypropylene block copolymer, calcium dodecyl benzene sulfonate, sodium dodecyl sulfate, phenethyl phenol polyoxyethylene ether, phenethyl phenol polyoxyethylene polyoxypropylene ether and alkylphenol formaldehyde resin polyoxyethylene ether; the cosolvent is one or more of ethanol, ethylene glycol, glycerol, isopropanol, n-butanol, isobutanol and polyethylene glycol.
5. Use of a botanical pesticide as claimed in claim 1 for controlling an agricultural pest, characterized in that: the botanical insecticide is a microemulsion and comprises 1-20 parts of nicotine citrate, 1-10 parts of solvent, 2-10 parts of emulsifier, 1-5 parts of cosolvent and 55-95 parts of water in parts by mass; the solvent is one or more of limonene, cyclohexanone, propylene glycol, dipropylene glycol, ethylene glycol methyl ether and ethylene glycol ethyl ether; the emulsifier is one or more of fatty alcohol polyoxyethylene ether, alkylphenol polyoxyethylene polyoxypropylene ether, fatty acid polyoxyethylene ester, castor oil polyoxyethylene ether, fatty amine polyoxyethylene ether, propylene glycol block polyether, random block polyether, sorbitan fatty acid ester polyoxyethylene ether, polyoxyethylene sorbitan fatty acid ester, tristyrylphenol polyoxypropylene polyoxyethylene block polymer, fatty alcohol EO/PO block copolymer, alkyl glycoside, castor oil phosphate, fatty alcohol polyoxyethylene ether organic phosphate, polyoxyethylene polyoxypropylene block copolymer, calcium dodecyl benzene sulfonate, sodium dodecyl sulfate, phenethyl phenol polyoxyethylene ether, phenethyl phenol polyoxyethylene polyoxypropylene ether and alkylphenol formaldehyde resin polyoxyethylene ether; the cosolvent is one or more of ethanol, ethylene glycol, glycerol, isopropanol and polyethylene glycol.
6. Use of a botanical pesticide as claimed in claim 1 for controlling an agricultural pest, characterized in that: the botanical pesticide is missible oil, and comprises 1-20 parts of nicotine citrate, 2-10 parts of emulsifier and 70-97 parts of solvent in parts by mass; the emulsifier is one or more of phenylethylphenol formaldehyde resin polyoxyethylene ether, phenylethylphenol polyoxyethylene polyoxypropylene ether, fatty alcohol polyoxyethylene ether, alkylphenol polyoxyethylene polyoxypropylene ether, fatty acid polyoxyethylene ester, castor oil polyoxyethylene ether, fatty amine polyoxyethylene ether, propylene glycol block polyether, random block polyether, sorbitan fatty acid ester, polyoxyethylene sorbitan fatty acid ester, fatty alcohol EO/PO block copolymer, alkyl glycoside, castor oil phosphate ester, fatty alcohol polyoxyethylene ether organic phosphate ester, polyoxyethylene polyoxypropylene block copolymer, calcium dodecylbenzene sulfonate, sodium dodecyl sulfate and alkylphenol formaldehyde resin polyoxyethylene ether; the solvent is one or more of turpentine-based vegetable oil, fatty acid methyl ester, methyl oleate, ethyl oleate, lauryl oleate, rosin methyl ester, hydrogenated rosin methyl ester, rosin polyoxyethylene ester, sec-butyl acetate, gamma-butyrolactone, N-alkyl-pyrrolidone, acetophenone, dimethyl carbonate, dipropyl carbonate, imidazolone solvents, dimethyl octanoamide, dimethyl decanoamide, C8-C12 acid dimethylamide, C12 acid dimethylamide, C18 acid dimethylamide, lactic acid dimethylamide, mixed fatty chain (C6-C12) morpholinamide, isohexyl bisdimethylamide and solvent oil.
7. Use of a botanical pesticide as claimed in claim 1 for controlling an agricultural pest, characterized in that: the botanical pesticide is a seed dressing agent and comprises 1-15 parts of nicotine citrate, 1-5 parts of dispersing agent, 2-8 parts of wetting agent, 0.5-4 parts of film forming agent, 0.1-0.5 part of warning color, 0-5 parts of antifreezing agent and 62.5-95.4 parts of deionized water in parts by mass; the dispersing agent is one or more of polyoxyethylene ether, triethylhexyl phosphoric acid, sodium dodecyl sulfate, alkyl naphthalene naphthenate formaldehyde condensate, cresol sulfonic acid, sodium naphthalene sulfonate formaldehyde condensate, lignosulfonate, alkylphenol polyoxyethylene ether formaldehyde condensate sulfate, fatty alcohol polyoxyethylene ether sulfate, alkyl phosphate, fatty alcohol polyoxyethylene ether organic phosphate and polycarboxylate; the wetting agent is one or more of polyacrylate, nekal, polyoxyethylene alkylphenol ether, polyoxyethylene fatty alcohol ether, polyoxyethylene polyoxypropylene block copolymer, alkyl sulfate, alkyl naphthalene naphthenate, sulfonate, fatty acid ester sulfate and fatty alcohol polyoxyethylene ether organic phosphate; the film forming agent is one or more of sodium alginate, sodium carboxymethyl cellulose, soluble starch, gelatin and polyvinyl alcohol; the warning color is one or more of ponceau, gamboge, alizarin red, indigo, malachite green and cochineal red; the antifreezing agent is one or more of ethanol, ethylene glycol, propylene glycol, diethylene glycol, glycerol, polyethylene glycol and urea.
8. Use of a botanical pesticide as claimed in claim 1 for controlling an agricultural pest, characterized in that: the botanical insecticide is a granule, and comprises, by mass, 0.5-5 parts of nicotine citrate, 1-8 parts of a wetting agent, 0.1-5 parts of a dispersing agent, 0.1-4 parts of a binder and 78-98.3 parts of a carrier; the wetting agent is one or more of polyacrylate, nekal, polyoxyethylene alkylphenol ether, polyoxyethylene fatty alcohol ether, polyoxyethylene polyoxypropylene block copolymer, alkyl sulfate, sulfonate, fatty acid ester sulfate and fatty alcohol polyoxyethylene ether organic phosphate; the dispersing agent is one or more of fatty alcohol-polyoxyethylene ether, triethylhexyl phosphoric acid, sodium dodecyl sulfate, alkyl naphthalene naphthenate, an alkyl naphthalene naphthenate formaldehyde condensate, cresol sulfonic acid, a naphthol sulfonic acid formaldehyde condensate, lignosulfonate, alkylphenol polyoxyethylene ether formaldehyde condensate sulfate, fatty alcohol-polyoxyethylene ether sulfate, alkyl phosphate, fatty alcohol-polyoxyethylene ether phosphate and polycarboxylate; the binder is one or more of polyvinyl alcohol, polyethylene glycol, soluble starch, beta-cyclodextrin, gum arabic, soybean protein, gelatin and polyvinyl acetate; the carrier is one or more of calcium carbonate, white carbon black, perlite, vermiculite, attapulgite, silica sand, silica, talc, pyrophyllite, pumice and artificial granular carriers.
9. Use of a botanical pesticide as claimed in claim 1 for controlling an agricultural pest, characterized in that: the botanical pesticide is an oil suspending agent and comprises, by mass, 1-20 parts of nicotine citrate, 1-20 parts of an emulsifier, 1-10 parts of a dispersant, 0.5-2 parts of a thickening agent, 1-10 parts of an adsorbent and 38-95.5 parts of vegetable oil or mineral oil; the emulsifier is one or more of polyester compound, phenethyl phenol formaldehyde resin polyoxyethylene ether, phenethyl phenol polyoxyethylene polyoxypropylene ether, fatty alcohol polyoxyethylene ether, alkylphenol polyoxyethylene polyoxypropylene ether, fatty acid polyoxyethylene ester, castor oil polyoxyethylene ether, fatty amine polyoxyethylene ether, propylene glycol block polyether, random block polyether, sorbitan fatty acid ester, polyoxyethylene sorbitan fatty acid ester, fatty alcohol EO/PO block copolymer, polyethylene oxide block copolymer, alkyl glycoside, castor oil phosphate ester, fatty alcohol polyoxyethylene ether organic phosphate ester and polyoxyethylene polyoxypropylene block copolymer; the dispersant is one or more of polycarboxylate, naphthalene sulfonate, sodium lignin sulfonate and organic phosphate; the thickening agent is one or more of organic bentonite, magnesium aluminum silicate, xanthan gum and polyvinyl alcohol; the adsorbent is one or more of white carbon black, pyrophyllite, talcum powder, light calcium carbonate, kaolin and diatomite; the oil is one or more of turpentine, soybean oil, rapeseed oil, castor oil, palm oil, corn oil, epoxidized soybean oil, methyl oleate, ethyl oleate, butyl oleate, mineral oil, rosin-based vegetable oil, agricultural solvent complex ester, coconut oil methyl ester, rapeseed methyl oleate, methyl ester soybean oil and palm methyl oleate.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010094268.1A CN111226941B (en) | 2020-02-15 | 2020-02-15 | Botanical insecticide for preventing and controlling crop pests |
| PCT/CN2021/073001 WO2021159938A1 (en) | 2020-02-15 | 2021-01-21 | Botanical insecticide for preventing and controlling crop pests |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010094268.1A CN111226941B (en) | 2020-02-15 | 2020-02-15 | Botanical insecticide for preventing and controlling crop pests |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN111226941A CN111226941A (en) | 2020-06-05 |
| CN111226941B true CN111226941B (en) | 2021-08-20 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010094268.1A Active CN111226941B (en) | 2020-02-15 | 2020-02-15 | Botanical insecticide for preventing and controlling crop pests |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN111226941B (en) |
| WO (1) | WO2021159938A1 (en) |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| UA112974C2 (en) * | 2010-09-16 | 2016-11-25 | Джеі. Бі. Кемікалс Енд Фармасьютікалс Лімітид | NICOTINE COMPOSITION (OPTIONS) |
| CN103688935A (en) * | 2013-12-13 | 2014-04-02 | 云南省烟草公司红河州公司 | Preparation method of nicotinic type biological pesticide by using waste fresh tobacco leaves |
| CN106305722A (en) * | 2016-08-05 | 2017-01-11 | 刘粟 | Method for preparing pesticide from tobacco leaf extract |
| KR20200037220A (en) * | 2017-06-26 | 2020-04-08 | 누드 니코틴 인코포레이티드 | Nicotine salt and its preparation and use method |
| CN109288115A (en) * | 2018-10-16 | 2019-02-01 | 云南拓宝科技有限公司 | A kind of nicotine salt and preparation method thereof of solventless method preparation |
-
2020
- 2020-02-15 CN CN202010094268.1A patent/CN111226941B/en active Active
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2021
- 2021-01-21 WO PCT/CN2021/073001 patent/WO2021159938A1/en active Application Filing
Also Published As
| Publication number | Publication date |
|---|---|
| WO2021159938A1 (en) | 2021-08-19 |
| CN111226941A (en) | 2020-06-05 |
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