CN111226936A

CN111226936A - Bactericide composition containing kasugamycin and isotianil

Info

Publication number: CN111226936A
Application number: CN201910390953.6A
Authority: CN
Inventors: 黄宇龙
Original assignee: Jiangxi Zhongxun Agrochemical Co ltd
Current assignee: Jiangxi Zhongxun Agrochemical Co ltd
Priority date: 2018-11-28
Filing date: 2019-05-11
Publication date: 2020-06-05

Abstract

The invention discloses a pesticide composition containing kasugamycin and isotianil, which comprises common auxiliaries, kasugamycin and isotianil, wherein the weight part ratio of the kasugamycin to the isotianil in the pesticide composition is 1: 1-1: 200, and the weight percentage of the total weight of the kasugamycin and the isotianil in the pesticide composition is 0.5-70%. After the kasugamycin and the isotianil are mixed, the composition has obvious synergistic effect on crop diseases such as rice blast, bacterial blight, bacterial rice blight and the like, can reduce the using amount of pesticides, reduce the using cost, relieve the pollution to the environment, delay the generation of drug resistance of pathogenic bacteria and prolong the service life of the pesticides.

Description

Bactericide composition containing kasugamycin and isotianil

Technical Field

The invention belongs to the technical field of pesticides, and particularly relates to a bactericide composition containing kasugamycin and isotianil and application thereof. Is used for preventing and treating crop diseases such as rice blast, bacterial blight, wheat sharp eyespot and the like.

Technical Field

Kasugamycin belongs to an amino glycoside substance, has strong penetrability to plants (rice), can be quickly absorbed by the plants and conducted to the whole plants, has an inhibiting effect on some gram-positive and gram-negative bacteria in vivo, has an action mechanism of mainly interfering the metabolism of amino acid of a thallus esterase system and obviously influencing the synthesis of protein, so that hypha in the rice plant becomes enlarged and deformed after contacting with a medicament, stops growing, branches at the transverse side and granulizes cytoplasm, and has the effects of controlling the extension of lesion spots and the appearance of new lesions.

Isotianil has strong plant induction activity, is applied preventively or is used in the early period of onset, and various biological factors and non-biological factors can activate the defensive resistance reaction of plants, namely system induction resistance, and influence multiple links of the life history of pathogenic bacteria, so that the plants can generate broad-spectrum self-protection effect on various fungi, bacteria, insects and viruses.

The reasonable compounding or mixing of the chemical bactericide has the advantages of improving the control effect, prolonging the pesticide application period, reducing the dosage, reducing the risk of phytotoxicity, reducing residues, delaying the occurrence and development of drug resistance and drug resistance of plant germs and the like, so that the selection of the proper compounding or mixing of the bactericide is one of the most effective and economical methods for solving the problems; however, because the action mechanism between pesticide blending is complex, whether the synergy is influenced by various factors after combination is verified only by depending on experimental results, and the expectation cannot be carried out.

The price of developing a new product of bactericide is high, the period is long, and compared with the price of developing and researching a high-efficiency, low-toxicity and low-residue compound and mixture, the investment is small, the development period is short, the attention at home and abroad is paid to the development, and the development strength is increased at a large margin.

The kasugamycin and the isotianil have different action mechanisms, and a single agent is easy to generate drug resistance, the dosage is larger and larger, the bactericidal spectrum is narrower, and the practical requirements cannot be met.

Disclosure of Invention

The invention relates to a bactericide composition containing kasugamycin and isotianil, which is a pesticide active ingredient composition and application thereof. The invention provides a solution for preventing and controlling crop diseases, provides a solution for managing pathogen resistance, and provides a solution for reducing the use of pesticides.

The technical scheme of the invention is as follows: the effective components of the pesticide composition comprise kasugamycin and isotianil, the weight part ratio of the kasugamycin to the isotianil in the pesticide composition is 1: 1-1: 200, the total weight of the kasugamycin and the isotianil in the pesticide composition is 0.5-70%, and the balance is auxiliary components acceptable and allowable in pesticide.

Further, the preferable weight part ratio of kasugamycin to isotianil is 1: 1-1: 80.

The pesticide composition can be prepared into any dosage form suitable for agricultural production, and the better dosage forms comprise water suspending agent, dispersible oil suspending agent, water dispersible granule, wettable powder and the like.

When the pesticide composition is prepared into various application formulations, the pesticide composition also comprises various acceptable auxiliary components which are allowed to be used in the pesticide besides the effective active components.

The auxiliary components include, but are not limited to, solvents, emulsifiers, wetting agents, dispersants, stabilizers, antifreeze agents, thickeners, antifoaming agents, synergists, preservatives, fillers, and the like, which are commonly used or allowed to be used in pesticide formulations, and are not particularly limited, and specific components and amounts thereof are determined by experiments according to formulation requirements.

The production process of various application formulations of the pesticide composition belongs to the prior known technology, and is not described in detail herein.

The aqueous suspension agent comprises the following components in percentage by weight: 0.01-20% of kasugamycin, 0.1-80% of isotianil, 0.1-18% of a dispersant, 0.1-6% of an antifreezing agent, 0.1-2% of a thickening agent and the balance of a deionized water carrier to 100%. The specific production steps are as follows: mixing the effective components with other auxiliary agents, performing high-speed shearing dispersion and sanding in a sand mill, and forming a high-dispersion stable suspension system in a water system medium to prepare the water suspending agent with corresponding weight percentage content, wherein the total active component content of the water suspending agent is not higher than 60%.

The dispersible oil suspending agent comprises the following components in percentage by weight: 0.01-20% of kasugamycin, 0.1-80% of isotianil, 0.1-15% of a dispersant, 0.1-4% of an antifreezing agent, 0.1-3% of a thickening agent and the balance of a liquid carrier to 100%. The specific production steps are as follows: mixing the effective components with other auxiliary agents, and forming a highly-dispersed and stable suspension system in a non-aqueous medium after high-speed shearing dispersion and sanding in a sand mill to prepare the dispersible oil suspending agent with corresponding weight percentage content, wherein the total active component content of the dispersible oil suspending agent is not higher than 60%.

The dosage form of the water dispersible granule comprises the following components in percentage by weight: 0.01-20% of kasugamycin, 0.1-80% of isotianil, 0.1-17% of a dispersant, 0.1-10% of a wetting agent, 0.1-10% of a disintegrating agent and the balance of a solid carrier to 100%. The specific production steps are as follows: the effective components and other auxiliary agents are uniformly mixed, crushed by an ultramicro jet mill, kneaded, added into a fluidized bed granulation dryer for granulation, drying and screening to prepare the water dispersible granules with corresponding weight percentage content, wherein the total active component content of the water dispersible granules is not higher than 70%.

The application frequency and the application amount of the pesticide composition provided by the invention slightly change along with the changes of crops, germs and weather conditions, and the purpose of effective prevention and treatment can be achieved by using a proper dosage.

The binary combination of the invention can solve the defects of single or binary use of the bactericide, delay the generation of resistance of germs to compounds, and the composition has synergistic response, such as crop diseases of rice blast, bacterial leaf blight, bacterial rice blight, wheat sharp eyespot and the like. Has obvious synergistic response and can provide an excellent solution for preventing and controlling crop germs.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more concise and clear, the present invention is described with reference to the following specific examples, but the present invention is by no means limited to these examples. The following description is only a preferred embodiment of the present invention, and is only for the purpose of describing the present invention, and should not be construed as limiting the scope of the present invention. It should be understood that any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Indoor virulence determination example

The test refers to 'indoor bioassay test criteria for pesticides', a pot inoculation method is adopted, 8-10 seeds are sown in each pot, each concentration is repeated five times, and each treatment is 5 pots in total. When the leaf stage is as long as 5, the medicine is treated, and the blank is treated by clear water. Treating with the preparation for 5 days, washing surface spore/mycelium with sterile water, adding Tween 80, and making into 1 × 10⁶The spore/hypha concentration is evenly sprayed and inoculated on the rice leaves by a throat sprayer. After inoculation, the seedlings were placed in a climatic environment.

Executing agricultural industry standard ' pesticide indoor bioassay test criterion ' of the people's republic of China by grading standard, calculating prevention and treatment effects by disease index, performing statistical analysis by DPS data processing software, and calculating EC of each medicament₅₀Then, the co-toxicity coefficient (CTC) was calculated according to the method of Sun Yunpei Specification, page 2/9, page 4.

The grading method comprises the following steps:

level 0: the whole plant is disease-free;

level 1: brown spot disease is appeared;

and 3, level: typical fusiform disease spots appear, and the area of the disease spots accounts for less than 5 percent of the whole leaf;

and 5, stage: typical scabs, the area of the scab accounts for 6 to 25 percent of the whole leaf;

and 7, stage: typical scabs, the area of the scab accounts for 26% -30% of the whole leaf;

and 9, stage: typical lesion spots, the area of the lesion spots accounts for more than 50% of the whole leaf.

Respectively establishing virulence regression equations by taking the logarithmic value of the medicament concentration (mg/L) as an independent variable x and the probability value of correcting the death rate as a dependent variable y, and calculating the EC of a single dose and the EC of mixed doses in each proportion by adopting DPS software₅₀And the co-toxicity coefficient of the mixed agent. According to the Sun Yunpei method, the co-toxicity coefficient (CTC) is more than or equal to 120 and shows a synergistic effect; the co-toxicity coefficient (CTC) is less than or equal to 80, and the antagonism is shown; 80 < co-toxicity coefficient (CTC) < 120 showed additive effects. Measured virulence index (ATI) ═ standard agent EC₅₀Reagent for test EC₅₀) X 100. Theoretical virulence index (TTI) ═ (virulence index of A. times the percentage of A in the mixture) (virulence index of B. times. percent B in the mixture). Co-toxicity coefficient (CTC) ═ mix measured virulence index (ATI)/mix theoretical virulence index (TTI)]×100。

TABLE 1 indoor toxicity test results of kasugamycin and isotianil mixed for Pyricularia oryzae as rice blast pathogen

Treatment formulations	EC₅₀(mg/L)	Actual ATI	Theoretical ATI	CTC
					Kasugamycin	0.5845	100
Isotianil	4.8425	12.07
					Kasugamycin: isotianil (1: 1)	0.8567	68.23	56.04	121.76
Kasugamycin: isotianil (1: 10)	2.0362	28.71	20.06	143.07
					Kasugamycin: isotianil (1: 20)	2.2941	25.48	16.26	156.72
Kasugamycin: isotianil (1: 40)	2.5214	23.18	14.21	163.08
					Kasugamycin: isotianil (1: 60)	2.3865	24.49	13.51	181.26
Kasugamycin: isotianil (1: 80)	2.2569	25.90	13.16	196.86
					Kasugamycin: isotianil (1:100)	2.5613	22.82	12.94	176.34
kasugamycin: isotianil (1: 150)	3.2941	17.74	12.65	140.24
					Kasugamycin: isotianil (1: 200)	3.6813	15.88	12.51	126.94

As can be seen from Table 1, the kasugamycin and isotianil are mixed and matched in the range of 1: 1-200 with the pyricularia oryzae as a rice blast pathogen, and the co-toxicity coefficient (CTC) is greater than 120, which shows that the synergistic effect is shown.

Table 2: indoor toxicity determination result of kasugamycin and isotianil mixed on xanthomonas oryzae as pathogen of rice bacterial-blight

Treatment formulations	EC₅₀(mg/L)	Actual ATI	Theoretical ATI	CTC
					Kasugamycin	2.5651	100
Isotianil	3.1054	82.60
					Kasugamycin: isotianil (1: 1)	2.3332	109.94	91.30	120.41
Kasugamycin: isotianil (1: 10)	2.2851	112.25	84.18	133.34
					Kasugamycin: isotianil (1: 20)	2.2941	111.81	83.43	134.02
Kasugamycin: isotianil (1: 40)	2.3865	107.48	83.03	129.46
					Kasugamycin: isotianil (1: 60)	2.3865	107.48	82.89	129.68
Kasugamycin: isotianil (1: 80)	2.4629	104.15	82.82	125.76
					Kasugamycin: isotianil (1: 100)	2.5618	100.13	82.77	120.97
Kasugamycin: isotianil (1: 150)	2.5618	100.13	82.72	121.05
					Kasugamycin: isotianil (1: 200)	2.5841	99.26	82.69	120.05

As can be seen from Table 2, the kasugamycin and isotianil are mixed and matched with xanthomonas oryzae, which is a pathogen of rice bacterial blight, in the range of 1: 1-200, and the co-toxicity coefficient (CTC) is greater than 120, which shows that the synergistic effect is shown.

Table 3: indoor toxicity determination result of kasugamycin and isotianil mixed and matched wheat rhizoctonia cerealis as pathogen

As can be seen from Table 3, the kasugamycin and isotianil are mixed and matched with Rhizoctonia cerealis which is a wheat rhizoctonia solani pathogen in the range of 1: 1-200, and the co-toxicity coefficient (CTC) is greater than 120, which shows that the synergistic effect is shown.

Table 4: indoor toxicity determination result of kasugamycin and isotianil mixed on rice bacterial Valley blast

Treatment formulations	EC₅₀(mg/L)	Actual ATI	Theoretical ATI	CTC
					KasugamycinVegetable extract	0.8656	100
Isotianil	1.6515	52.41
					Kasugamycin: isotianil (1: 1)	0.9258	93.50	76.21	122.69
Kasugamycin: isotianil (1: 2)	1.0025	86.34	68.28	126.46
					Kasugamycin: isotianil (1: 4)	1.0692	80.96	61.93	130.72
Kasugamycin: isotianil (1: 6)	1.1495	75.30	59.21	127.18
					Kasugamycin: isotianil (1: 8)	1.2159	71.19	57.70	123.38
Kasugamycin: isotianil (1: 10)	1.2695	68.18	56.74	120.17

As can be seen from Table 4, the kasugamycin and isotianil are mixed and matched in the range of 1: 1-10 with the bacterial rice valepotriates, and the co-toxicity coefficients (CTC) are all larger than 120, which shows that the synergistic effect is shown.

Formulation examples of the formulations

Preparation 1: 15% kasugamycin isotianil water suspension

15% of kasugamycin isotianil water suspending agent, which comprises the following components in percentage by weight: 0.1% of kasugamycin, 14.9% of isotianil, 2% of saponin powder, 3% of sorbitol oleate, 0.5% of sodium alginate, NP-42%, 2% of calcium lignosulfonate, 2% of propylene glycol, 0.1% of xanthan gum and deionized water for supplementing to 100%.

Preparation 2: 10.5% kasugamycin isotianil dispersed oil suspending agent

10.5% of kasugamycin isotianil dispersible oil suspending agent, which comprises the following components in percentage by weight: 0.5% of kasugamycin, 10% of isotianil, 10% of sec-butyl acetate, 5% of cyclohexanone, 5% of polyoxyethylene polyoxypropylene ether block copolymer, 4% of sodium dodecyl benzene sulfonate, 2% of glycerol, 3% of white carbon black and methyl oleate, wherein the balance is up to 100%.

Preparation 3: 55% kasugamycin isotianil water dispersible granule

The 55% kasugamycin isotianil water dispersible granule comprises the following components in percentage by weight: 0.5% of kasugamycin, 54.5% of isotianil, 10% of fatty acid ester sulfate, 5% of sodium polycarboxylate, 6% of starch dextrin, 7% of sodium lignosulfonate, 3% of chitosan and 100% of white carbon black.

(III) field Effect examples

According to the blank disease condition, the seed leaves are graded and investigated, and the following grading method is adopted

Grade 0, no disease;

grade 1, the lesion area accounts for less than 5% of the whole leaf area;

grade 3, the lesion area accounts for 6 to 15 percent of the whole leaf area;

grade 5, the lesion area accounts for 16 to 25 percent of the whole leaf area;

7 grade, the lesion area accounts for 26 to 50 percent of the whole leaf area;

grade 9, the lesion area accounts for more than 50% of the whole leaf area.

Calculating disease index and preventing and treating effect according to the survey data, calculating the disease index according to the formula (1), and reserving the calculation result

The last two decimal places:

wherein X represents disease index; n; representing the number of leaves of each grade of disease; i represents a relative grade value; n represents the total number of leaves investigated.

The prevention and treatment effect is calculated according to the formula (2):

in the formula:

p represents the control effect in percent (%);

CK represents placebo disease index;

PT indicates the index of disease for drug treatment.

TABLE 5 application of the composition of the present invention in prevention and treatment of rice diseases

As can be seen from the table 5, compared with the single use of the composition for preventing and treating rice blast, rice bacterial blight and rice bacterial Valley blast, kasugamycin and isotianil, the composition has the advantages of remarkably improving the effect, reducing the use amount and comprehensively preventing and treating various diseases.

TABLE 6 application of the composition of the present invention in wheat disease control

As can be seen from the table 6, compared with the single use of the composition of kasugamycin and isotianil for preventing and treating the wheat sharp eyespot and the wheat basal rot, the composition has the advantages of obviously improving the effect, reducing the use amount and comprehensively preventing and treating various diseases.

TABLE 7 application of the composition of the present invention in corn disease control

As can be seen from the table 7, compared with the single use of the composition for preventing and treating the corn northern leaf blight and the corn sheath blight, the composition has the advantages that the effect is obviously improved, the use amount is reduced, and meanwhile, the effects of comprehensively preventing and treating various diseases are achieved.

(IV) determination of resistance development

The concentration of the test medicament is 50-60% of the lethal dose of the pathogenic bacteria, the growth bacteria source is continuously adopted to continue the propagation test, and the pathogenic bacteria of 5 generations are repeatedly processed in the way. The liquid medicine only containing the auxiliary agent is treated as a blank control. The respective agents are in the 1 st generation andhalf lethal concentration EC at passage 5₅₀EC against passage 1 and passage 5 pathogens by comparison of treatment Agents₅₀. To evaluate the change of the resistance of the test bacteria to the test agent.

TABLE 8 determination of the development of resistance of the compositions according to the invention to pathogenic bacteria of crop diseases

As can be seen from Table 8, the kasugamycin and the iprovalicarb mixed combination of the invention can effectively delay the generation of drug resistance of pathogenic bacteria.

Claims

1. A pesticide composition containing kasugamycin and isotianil is characterized in that: the effective components comprise kasugamycin and isotianil, and the weight portion ratio of the kasugamycin to the isotianil in the pesticide composition is 1: 1-1: 200.

2. The pesticide composition containing kasugamycin and isotianil according to claim 1, which is characterized in that: the weight portion ratio of kasugamycin to isotianil in the pesticide composition is 1: 1-1: 100.

3. The pesticide composition containing kasugamycin and isotianil according to claim 1, which is characterized in that: the weight parts of the kasugamycin and the isotianil in the pesticide composition are respectively 1:1, 1:2, 1:4, 1:6, 1:8, 1:10, 1:20, 1:40 or 1: 80.

4. The pesticide composition containing kasugamycin and isotianil according to claim 1, which is characterized in that: the weight parts of the kasugamycin and the isotianil in the pesticide composition are respectively 1:1, 1:2, 1:4, 1:6, 1:8, 1:10, 1:20, 1:40 or 1:80, the total weight of the kasugamycin and the isotianil in the pesticide composition is 0.5-70%, and the balance is auxiliary components acceptable and allowed to be used in pesticides.

5. The pesticide composition containing kasugamycin and isotianil according to claim 1, which is characterized in that: the dosage form of the suspension is one of water suspension, dispersible oil suspension and water dispersible granules.

6. The pesticide composition containing kasugamycin and isotianil according to claim 1, which can be processed into a 15% kasugamycin-isotianil water suspension, and comprises the following components in percentage by weight: 0.1% of kasugamycin, 14.9% of isotianil, 2% of saponin powder, 3% of sorbitol oleate, 0.5% of sodium alginate, NP-42%, 2% of calcium lignosulfonate, 2% of propylene glycol, 0.1% of xanthan gum and deionized water for supplementing to 100%.

7. The pesticide composition containing kasugamycin and isotianil according to claim 1, which is capable of being processed into 10.5% of dispersible oil suspending agent of kasugamycin and isotianil, and comprises the following components in percentage by weight: 0.5% of kasugamycin, 10% of isotianil, 10% of sec-butyl acetate, 5% of cyclohexanone, 5% of polyoxyethylene polyoxypropylene ether block copolymer, 4% of sodium dodecyl benzene sulfonate, 2% of glycerol, 3% of white carbon black and methyl oleate, wherein the balance is up to 100%.

8. The pesticide composition containing kasugamycin and isotianil according to claim 1, which is capable of being processed into 55% kasugamycin-isotianil water dispersible granules, and comprises the following components in percentage by weight: 0.5% of kasugamycin, 54.5% of isotianil, 10% of fatty acid ester sulfate, 5% of sodium polycarboxylate, 6% of starch dextrin, 7% of sodium lignosulfonate, 3% of chitosan and 100% of white carbon black.

9. The application of the pesticide composition containing kasugamycin and isotianil as claimed in claim 1 in preventing and treating crop diseases, including rice blast, bacterial blight, bacterial rice blight and wheat sharp eyespot.