CN111226771A - Deep liquid flow culture method for soilless culture oxygen carrier - Google Patents
Deep liquid flow culture method for soilless culture oxygen carrier Download PDFInfo
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- CN111226771A CN111226771A CN202010162398.4A CN202010162398A CN111226771A CN 111226771 A CN111226771 A CN 111226771A CN 202010162398 A CN202010162398 A CN 202010162398A CN 111226771 A CN111226771 A CN 111226771A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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Abstract
The invention discloses a deep liquid flow culture method for soilless culture oxygen carriers, which comprises the following steps: A. taking an emulsifier and a small amount of water, fully stirring, slowly adding an oxygen carrier while stirring, and stirring to form O/W type emulsion; B. slowly adding the emulsifier and the oxygen carrier emulsion which are mixed and stirred into the conventional culture solution under the condition of stirring; C. adding oxygen carrier into conventional culture solution, wherein the amount of oxygen carrier is 1-20% of that of conventional culture solution, selecting n-hexane, n-dodecane, soybean oil and toluene, and adding 0.1-5% of liposoluble bacteriostatic agent. The invention effectively increases the oxygen supply time by the oxygen supply mode, changes the traditional culture solution flowing for 15 minutes every hour into the culture solution flowing for 15 minutes every 3-6 hours, ensures that the local oxygen supply is more uniform, is beneficial to quickly obtaining nutrient substances from root systems, increases the stress resistance of soilless culture, improves the yield and reduces the energy consumption.
Description
Technical Field
The invention relates to the technical field of soilless culture, in particular to a deep liquid flow culture method for an oxygen carrier in soilless culture.
Background
Soilless culture is divided into four types, namely thin-film flowing water, spray culture, deep liquid flow culture and still water flower culture, in the four modes, the important basis of classification is the supply condition of the root system to oxygen, and compared with soil culture, the most important difference of water culture is the oxygen supply mode of the root system, so that the function supply of meeting the root oxygen becomes important.
The oxygen carrier artificial red blood cells, the modified hemoglobin solution, the perfluorocarbon compound emulsion, the dissolved oxygen organic solvent such as n-hexane, n-dodecane, soybean oil and toluene and the like greatly improve the solubility of oxygen. Oxygen carriers have been used in fermentation, called two-phase liquid fermentation, such as (CN 103820520B) a fermentation method for high yield of natural astaxanthin, in which the oxygen carriers are simply added, and although the oxygen dissolving efficiency is improved, there is still room for improvement.
The oxygen transfer efficiency is determined by the contact area of the two media, the oxygen transfer efficiency into water is related to the contact degree of the gas-liquid interface, and also in the dissolved oxygen transfer process mediated by the oxygen carrier, depending on the transfer efficiency of the two layers, the contact condition of the air and the oxygen carrier or the air and the water and the contact condition of the oxygen carrier and the water, because the oxygen carrier is all water-insoluble substances, the contact with the water is preferably increased by changing the oxygen carrier into emulsion, if the oxygen carrier can be dispersed into small liquid drops with the diameter of about one micron, the contact area of the oxygen carrier to the water is more than 300 times, and the increase of the contact area necessarily increases the oxygen transfer efficiency, but if the emulsion is formed, the density of the oxygen carrier is preferably quite good compared with the water density because the perfluorocarbon compounds have the water density of 1.8-2.5 times, and other media such as oxygen carrier artificial red blood cells, carbon dioxide, The density of the modified hemoglobin solution and dissolved oxygen organic solvents such as n-hexane, n-dodecane, soybean oil, toluene and the like is basically 0.85-1.1 times of the water density, so that the modified hemoglobin solution and the dissolved oxygen organic solvents are suitable to be used as oxygen carriers capable of forming emulsions, the modified hemoglobin solution and the dissolved oxygen organic solvents are used for root system culture of soilless culture, the modified hemoglobin solution and the dissolved oxygen organic solvents can greatly influence the dissolved oxygen of root systems of the soilless culture, and further influence the change of a culture mode.
Disclosure of Invention
The invention aims to provide a deep liquid flow culture method of an oxygen carrier for soilless culture, which aims to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme: the deep liquid flow culture method of soilless culture oxygen carrier includes the following steps:
A. taking an emulsifier and a small amount of water, fully stirring, slowly adding an oxygen carrier while stirring, and stirring to form O/W type emulsion;
B. slowly adding the emulsifier and the oxygen carrier emulsion which are mixed and stirred into the conventional culture solution under the condition of stirring;
C. adding an oxygen carrier into a conventional culture solution, wherein the amount of the oxygen carrier is 1-20% of that of the conventional culture solution, selecting n-hexane, n-dodecane, soybean oil and toluene, and adding 0.1-5% of a fat-soluble bacteriostatic agent;
D. taking emulsifier with the total liquid amount of 0.5-2% and a small amount of water (the water consumption is more than the amount of oxygen carrier), fully stirring, slowly adding oxygen carrier while stirring, wherein the amount of oxygen carrier accounts for 2-20% of the total liquid amount, and stirring to form O/W type emulsion;
E. slowly adding the emulsifier and the oxygen carrier emulsion which are mixed and stirred into the conventional culture solution under the stirring condition, and preventing the precipitation;
F. the vessel was stirred and oxygenated to a dissolved oxygen concentration of 50-80% of the saturated dissolved oxygen concentration, measured with an oxygen saturation concentration meter and controlled to a ventilation of 1.2vvm, and pumped through a line into a fluid culture tank.
Preferably, the step a may also be: the emulsifier and the oxygen carrier are mixed first, stirred and then water is slowly added while stirring until the amount of water exceeds the amount of emulsifier mixed with the oxygen carrier.
Preferably, the step C may also be: adding oxygen carrier into conventional culture solution, wherein the amount of oxygen carrier is 1-20% of that of conventional culture solution, and selecting artificial red blood cells and modified hemoglobin solution.
Preferably, the step D may also be: mixing the emulsifier and the oxygen carrier, stirring, slowly adding water while stirring until the water amount exceeds the mixing amount of the emulsifier and the oxygen carrier, measuring the pH value, and controlling the pH value of the culture solution to be 5.5-7.5 by adding 2mol/L NaOH and 2mol/L HCl in a flowing manner, wherein the pH value is the same as that of the hydroponic solution.
Preferably, the emulsifier in step D is one or more of polyoxyethylene (20) sorbitan tristearate (HLB 11.0), polyoxyethylene (4) monolaurate (HLB 13.3), polyoxyethylene (20) sorbitan monostearate (HLB 14.9), polyoxyethylene (20) sorbitan monooleate (HLB 15.0), polyoxyethylene (20) monolaurate (HLB 16.3), sucrose distearate (HLB 30.0) or sucrose monolaurate (HLB 15.0).
Preferably, the container in step F is a water culture liquid concentration pool or a flower culture tank, and the water culture liquid is filled with oxygen once during normal culture, the water culture liquid can be used for 3 to 12 hours, every 3 to 12 hours, the liquid is pumped into the container to be stirred and oxygenated for 10 to 30 minutes, or the liquid is directly allowed to flow for 10 to 30 minutes.
Compared with the prior art, the invention has the following beneficial effects:
by adopting the oxygen supply mode, the oxygen supply time is effectively prolonged, and the traditional method that the culture solution flows for 15 minutes every hour is changed into the method that the culture solution flows for 15 minutes every 3-6 hours; moreover, the oxygen carrier is changed into the emulsion, so that the oxygen is supplied to local parts more uniformly, the root system can obtain nutrient substances quickly, and the oxygen supply effect is better than that of the two-phase fermentation; especially, under the condition that the liquid where the root system of the soilless culture is in is lack of stirring, the oxygen is fully supplied to the root system in a long distance and a large range, the stress resistance of the soilless culture is greatly increased, the yield is improved, and the energy consumption is reduced.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The first embodiment is as follows:
the deep liquid flow culture method of soilless culture oxygen carrier includes the following steps:
A. taking an emulsifier and a small amount of water, fully stirring, slowly adding an oxygen carrier while stirring, and stirring to form O/W type emulsion;
B. slowly adding the emulsifier and the oxygen carrier emulsion which are mixed and stirred into the conventional culture solution under the condition of stirring;
C. adding an oxygen carrier into a conventional culture solution, wherein the amount of the oxygen carrier is 1-20% of that of the conventional culture solution, selecting normal hexane, n-dodecane, soybean oil and toluene, and adding 0.1-5% of a fat-soluble bacteriostatic agent to prevent microorganisms in the air from decomposing an organic oxygen carrier, so that the organic oxygen carrier can be suitable for a power failure state for a longer time, the stress resistance of soilless culture is increased, and under normal conditions, the improvement of oxygen exchange efficiency plays a good role in promoting the growth of a root system and the absorption of the root system to nutrient substances;
D. taking emulsifier with the total liquid amount of 0.5-2% and a small amount of water (the water consumption is more than the amount of oxygen carrier), fully stirring, slowly adding oxygen carrier while stirring, wherein the amount of oxygen carrier accounts for 2-20% of the total liquid amount, and stirring to form O/W type emulsion;
E. slowly adding the emulsifier and the oxygen carrier emulsion which are mixed and stirred into the conventional culture solution under the stirring condition, and preventing the precipitation;
F. the vessel was stirred and oxygenated to a dissolved oxygen concentration of 50-80% of the saturated dissolved oxygen concentration, measured with an oxygen saturation concentration meter and controlled to a ventilation of 1.2vvm, and pumped through a line into a fluid culture tank.
By the oxygen supply mode, the oxygen supply time is effectively prolonged, the culture solution flows for 15 minutes every hour in the prior art, and the culture solution flows for 15 minutes every 3-6 hours, so that the local oxygen supply is more uniform, the root system can quickly obtain nutrient substances, the stress resistance of the soilless culture is improved, the yield is improved, and the energy consumption is reduced.
The emulsifier in step D is a mixture of four of polyoxyethylene (20) sorbitan tristearate (HLB 11.0), polyoxyethylene (4) monolaurate (HLB 13.3), polyoxyethylene (20) sorbitan monostearate (HLB 14.9) and polyoxyethylene (20) sorbitan monooleate (HLB 15.0), and the ratio between polyoxyethylene (20) sorbitan tristearate (HLB 11.0), polyoxyethylene (4) monolaurate (HLB 13.3), polyoxyethylene (20) sorbitan monostearate (HLB 14.9) and polyoxyethylene (20) sorbitan monooleate (HLB 15.0) is 0.7: 1.2: 1.5: 1.
and F, filling oxygen once in the container in the step F, using for 3-12 hours, pumping the liquid to the water culture liquid concentration pool every 3-12 hours, stirring and oxygenating for 10-30 minutes, pumping the liquid when the pH value of the culture solution deviates from a set value of 0.5-1 and the salt concentration conductivity deviates from a set value of 0.2-1, stirring and oxygenating, adjusting the content of nutrient substances and the pH value, controlling the total salt content to be 0.83-4.2G/L and the conductivity to be 0.83-4.2ms/cm, and replacing the culture solution or supplementing elements when the total salt content exceeds the maximum value.
Example two:
the deep liquid flow culture method of soilless culture oxygen carrier includes the following steps:
A. firstly mixing the emulsifier and the oxygen carrier, stirring, and then slowly adding water during stirring until the water amount exceeds the mixing amount of the emulsifier and the oxygen carrier;
B. slowly adding the emulsifier and the oxygen carrier emulsion which are mixed and stirred into the conventional culture solution under the condition of stirring;
C. adding an oxygen carrier into a conventional culture solution, wherein the amount of the oxygen carrier is 1-20% of that of the conventional culture solution, selecting an artificial erythrocyte and a modified hemoglobin solution, and packaging the oxygen carrier to prevent the decomposition of single microorganisms in the liquid or lightly so as to ensure that the oxygen carrier can be reused for a longer time;
D. firstly mixing an emulsifier and an oxygen carrier, stirring, then slowly adding water during stirring until the water amount exceeds the mixing amount of the emulsifier and the oxygen carrier, measuring the pH value of the mixture, and controlling the pH value of a culture solution to be 5.5-7.5 by adding 2mol/L NaOH and 2mol/L HCl in a flowing manner, wherein the pH value is the same as that of a hydroponic solution;
E. slowly adding the emulsifier and the oxygen carrier emulsion which are mixed and stirred into the conventional culture solution under the stirring condition, and preventing the precipitation;
F. the vessel was stirred and oxygenated to a dissolved oxygen concentration of 50-80% of the saturated dissolved oxygen concentration, measured with an oxygen saturation concentration meter and controlled to a ventilation of 1.2vvm, and pumped through a line into a fluid culture tank.
By the oxygen supply mode, the oxygen supply time is effectively prolonged, the culture solution flows for 15 minutes every hour in the prior art, and the culture solution flows for 15 minutes every 3-6 hours, so that the local oxygen supply is more uniform, the root system can quickly obtain nutrient substances, the stress resistance of the soilless culture is improved, the yield is improved, and the energy consumption is reduced.
The emulsifier in step D is a mixture of four of polyoxyethylene (20) sorbitan monooleate (HLB 15.0), polyoxyethylene (20) monolaurate (HLB 16.3), sucrose distearate (HLB 30.0) and sucrose monolaurate (HLB 15.0), and the ratio between polyoxyethylene (20) sorbitan monooleate (HLB 15.0), polyoxyethylene (20) monolaurate (HLB 16.3), sucrose distearate (HLB 30.0) and sucrose monolaurate (HLB 15.0) is 1: 0.8: 1.2: 0.5.
and F, filling oxygen once, wherein the container in the step F is a flower culture tank, the container can be used for 3-12 hours, the liquid is directly allowed to flow for 10-30 minutes every 3-12 hours, when the pH value of the culture solution deviates from a set value by 0.5-1 and the salt concentration conductivity deviates from a set value by 0.2-1, the liquid is extracted, stirring and oxygenation are carried out, the content and the pH value of nutrient substances are adjusted, the total salt content is controlled to be 0.83-4.2G/L and the conductivity is controlled to be 0.83-4.2ms/cm, and when the maximum value is exceeded, the culture solution is replaced or elements are supplemented.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (6)
1. The deep liquid flow culture method of the soilless culture oxygen carrier is characterized in that: the method comprises the following steps:
A. taking an emulsifier and a small amount of water, fully stirring, slowly adding an oxygen carrier while stirring, and stirring to form O/W type emulsion;
B. slowly adding the emulsifier and the oxygen carrier emulsion which are mixed and stirred into the conventional culture solution under the condition of stirring;
C. adding an oxygen carrier into a conventional culture solution, wherein the amount of the oxygen carrier is 1-20% of that of the conventional culture solution, selecting n-hexane, n-dodecane, soybean oil and toluene, and adding 0.1-5% of a fat-soluble bacteriostatic agent;
D. taking emulsifier with the total liquid amount of 0.5-2% and a small amount of water (the water consumption is more than the amount of oxygen carrier), fully stirring, slowly adding oxygen carrier while stirring, wherein the amount of oxygen carrier accounts for 2-20% of the total liquid amount, and stirring to form O/W type emulsion;
E. slowly adding the emulsifier and the oxygen carrier emulsion which are mixed and stirred into the conventional culture solution under the stirring condition, and preventing the precipitation;
F. stirring and oxygenating the container to reach dissolved oxygen concentration 50-80% of saturated dissolved oxygen concentration, measuring with oxygen saturation concentration meter, controlling ventilation amount, and pumping into fluid culture tank via pipeline.
2. A soilless culture oxygen carrier deep stream culture method as claimed in claim 1, characterized in that: the step A can also be: the emulsifier and the oxygen carrier are mixed first, stirred and then water is slowly added while stirring until the amount of water exceeds the amount of emulsifier mixed with the oxygen carrier.
3. A soilless culture oxygen carrier deep stream culture method as claimed in claim 1, characterized in that: the step C can also be as follows: adding oxygen carrier into conventional culture solution, wherein the amount of oxygen carrier is 1-20% of that of conventional culture solution, and selecting artificial red blood cells and modified hemoglobin solution.
4. A soilless culture oxygen carrier deep stream culture method as claimed in claim 1, characterized in that: the step D may also be: mixing emulsifier and oxygen carrier, stirring, slowly adding water while stirring until the water amount exceeds the amount of emulsifier and oxygen carrier, measuring pH, and controlling pH of the culture solution to 5.5-7.5 by adding acid or alkali, and keeping the pH same as that of the hydroponic culture solution.
5. A soilless culture oxygen carrier deep stream culture method as claimed in claim 1, characterized in that: the emulsifier in step D is preferably one or more of polyoxyethylene (20) sorbitan tristearate (HLB 11.0), polyoxyethylene (4) monolaurate (HLB 13.3), polyoxyethylene (20) sorbitan monostearate (HLB 14.9), polyoxyethylene (20) sorbitan monooleate (HLB 15.0), polyoxyethylene (20) monolaurate (HLB 16.3), sucrose distearate (HLB 30.0) or sucrose monolaurate (HLB 15.0).
6. A soilless culture oxygen carrier deep stream culture method as claimed in claim 1, characterized in that: and F, the container in the step F is a water culture liquid concentration pool or a flower culture tank, and oxygen is filled once during normal culture, the container can be used for 3 to 12 hours, every 3 to 12 hours, the liquid is pumped out to the container and is stirred and oxygenated for 10 to 30 minutes, or the liquid is directly allowed to flow for 10 to 30 minutes.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101253847A (en) * | 2008-04-01 | 2008-09-03 | 中国农业大学 | Tomato tree type cultivation method preventing root system from decaying and preventing leaves from yellowing |
| CN102086445A (en) * | 2009-12-04 | 2011-06-08 | 山东新时代药业有限公司 | Method for increasing Gluconobacter oxydans biomass by fermenting with oxygen-carrier-added culture medium |
| US20180338440A1 (en) * | 2017-05-27 | 2018-11-29 | Robert Dearinger | Cloner |
| JP2019180252A (en) * | 2018-04-02 | 2019-10-24 | パナソニックＩｐマネジメント株式会社 | Plant cultivation device and plant cultivation method |
| CN110818479A (en) * | 2019-10-30 | 2020-02-21 | 通化力神保健品有限公司 | Ginseng culture solution and preparation method and application thereof |
-
2020
- 2020-03-10 CN CN202010162398.4A patent/CN111226771A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101253847A (en) * | 2008-04-01 | 2008-09-03 | 中国农业大学 | Tomato tree type cultivation method preventing root system from decaying and preventing leaves from yellowing |
| CN102086445A (en) * | 2009-12-04 | 2011-06-08 | 山东新时代药业有限公司 | Method for increasing Gluconobacter oxydans biomass by fermenting with oxygen-carrier-added culture medium |
| US20180338440A1 (en) * | 2017-05-27 | 2018-11-29 | Robert Dearinger | Cloner |
| JP2019180252A (en) * | 2018-04-02 | 2019-10-24 | パナソニックＩｐマネジメント株式会社 | Plant cultivation device and plant cultivation method |
| CN110818479A (en) * | 2019-10-30 | 2020-02-21 | 通化力神保健品有限公司 | Ginseng culture solution and preparation method and application thereof |
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Application publication date: 20200605 |